LOCUS       DARDHU010000001       162267 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000001 DARDHU010000000
VERSION     DARDHU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 162267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 162267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..162267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.001"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(12..1535)
                     /locus_tag="SDD03_000001"
     CDS             complement(12..1535)
                     /locus_tag="SDD03_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG4302485.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="SDD03_000002"
     CDS             1787..2968
                     /locus_tag="SDD03_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4302486.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SDD03_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SDD03_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG4302487.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="SDD03_000004"
     CDS             complement(5053..7764)
                     /locus_tag="SDD03_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4302488.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="SDD03_000005"
     CDS             complement(7774..8475)
                     /locus_tag="SDD03_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4302489.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="SDD03_000006"
     CDS             8661..9260
                     /locus_tag="SDD03_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4302490.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="SDD03_000007"
     CDS             9341..10354
                     /locus_tag="SDD03_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4302491.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="SDD03_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="SDD03_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG4302492.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="SDD03_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="SDD03_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG4302493.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="SDD03_000010"
     CDS             11802..13175
                     /locus_tag="SDD03_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4302494.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="SDD03_000011"
     CDS             13532..15676
                     /locus_tag="SDD03_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG4302495.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="SDD03_000012"
     CDS             complement(15728..16768)
                     /locus_tag="SDD03_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4302496.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="SDD03_000013"
     CDS             complement(17045..18847)
                     /locus_tag="SDD03_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4302497.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="SDD03_000014"
     CDS             complement(18861..19613)
                     /locus_tag="SDD03_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4302498.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="SDD03_000015"
     CDS             complement(19622..19984)
                     /locus_tag="SDD03_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4302499.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="SDD03_000016"
     CDS             complement(20160..21032)
                     /locus_tag="SDD03_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG4302500.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="SDD03_000017"
     CDS             complement(21058..21570)
                     /locus_tag="SDD03_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG4302501.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="SDD03_000018"
     CDS             complement(21708..22499)
                     /locus_tag="SDD03_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG4302502.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="SDD03_000019"
     CDS             complement(22554..23162)
                     /locus_tag="SDD03_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG4302503.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="SDD03_000020"
     CDS             complement(23155..23472)
                     /locus_tag="SDD03_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4302504.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="SDD03_000021"
     CDS             complement(23628..24356)
                     /locus_tag="SDD03_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG4302505.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="SDD03_000022"
     CDS             24515..25396
                     /locus_tag="SDD03_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4302506.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="SDD03_000023"
     CDS             complement(25633..26676)
                     /locus_tag="SDD03_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4302507.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="SDD03_000024"
     CDS             complement(26679..27347)
                     /locus_tag="SDD03_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4302508.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="SDD03_000025"
     CDS             27489..29825
                     /locus_tag="SDD03_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4302509.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="SDD03_000026"
     CDS             complement(29940..30251)
                     /locus_tag="SDD03_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302510.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="SDD03_000027"
     CDS             30432..31907
                     /locus_tag="SDD03_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG4302511.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="SDD03_000028"
     CDS             31954..32538
                     /locus_tag="SDD03_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG4302512.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="SDD03_000029"
     CDS             32540..32959
                     /locus_tag="SDD03_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG4302513.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="SDD03_000030"
     CDS             complement(33240..33524)
                     /locus_tag="SDD03_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG4302514.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="SDD03_000031"
     CDS             complement(33876..34586)
                     /locus_tag="SDD03_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG4302515.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="SDD03_000032"
     CDS             complement(34583..36055)
                     /locus_tag="SDD03_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4302516.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="SDD03_000033"
     CDS             36193..36666
                     /locus_tag="SDD03_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4302517.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="SDD03_000034"
     CDS             complement(36819..37370)
                     /locus_tag="SDD03_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4302518.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="SDD03_000035"
     CDS             37475..38005
                     /locus_tag="SDD03_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG4302519.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="SDD03_000036"
     CDS             complement(38488..38931)
                     /locus_tag="SDD03_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4302520.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="SDD03_000037"
     CDS             complement(38947..40107)
                     /locus_tag="SDD03_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4302521.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="SDD03_000038"
     CDS             40614..42314
                     /locus_tag="SDD03_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302522.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="SDD03_000039"
     CDS             complement(42416..43249)
                     /locus_tag="SDD03_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG4302523.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="SDD03_000040"
     CDS             complement(43553..45718)
                     /locus_tag="SDD03_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4302524.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="SDD03_000041"
     CDS             45978..48272
                     /locus_tag="SDD03_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG4302525.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="SDD03_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="SDD03_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG4302526.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="SDD03_000043"
     CDS             48923..49930
                     /locus_tag="SDD03_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4302527.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="SDD03_000044"
     CDS             49952..50971
                     /locus_tag="SDD03_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4302528.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SDD03_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SDD03_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG4302529.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="SDD03_000046"
     CDS             complement(52729..54150)
                     /locus_tag="SDD03_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4302530.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SDD03_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SDD03_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG4302531.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="SDD03_000048"
     CDS             complement(56561..57817)
                     /locus_tag="SDD03_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG4302532.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="SDD03_000049"
     CDS             58397..59698
                     /locus_tag="SDD03_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG4302533.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="SDD03_000050"
     CDS             complement(59767..60366)
                     /locus_tag="SDD03_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4302534.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="SDD03_000051"
     CDS             60620..61501
                     /locus_tag="SDD03_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4302535.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="SDD03_000052"
     CDS             61514..63124
                     /locus_tag="SDD03_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG4302536.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="SDD03_000053"
     CDS             complement(63201..63860)
                     /locus_tag="SDD03_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4302537.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="SDD03_000054"
     CDS             complement(64143..64808)
                     /locus_tag="SDD03_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG4302538.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SDD03_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SDD03_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG4302539.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SDD03_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SDD03_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG4302540.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SDD03_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SDD03_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG4302541.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="SDD03_000058"
     CDS             67122..67442
                     /locus_tag="SDD03_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG4302542.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="SDD03_000059"
     CDS             complement(67554..68423)
                     /locus_tag="SDD03_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4302543.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="SDD03_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="SDD03_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="SDD03_000061"
     CDS             complement(69819..70328)
                     /locus_tag="SDD03_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302544.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="SDD03_000062"
     CDS             complement(70503..72638)
                     /locus_tag="SDD03_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4302545.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="SDD03_000063"
     CDS             complement(72721..74376)
                     /locus_tag="SDD03_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4302546.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="SDD03_000064"
     CDS             complement(74378..74692)
                     /locus_tag="SDD03_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4302547.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="SDD03_000065"
     CDS             74862..75122
                     /locus_tag="SDD03_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG4302548.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="SDD03_000066"
     CDS             75136..75450
                     /locus_tag="SDD03_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG4302549.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="SDD03_000067"
     CDS             complement(76180..76734)
                     /locus_tag="SDD03_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG4302550.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="SDD03_000068"
     CDS             complement(76860..78788)
                     /locus_tag="SDD03_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG4302551.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="SDD03_000069"
     CDS             complement(79094..79810)
                     /locus_tag="SDD03_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG4302552.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SDD03_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SDD03_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG4302553.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SDD03_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SDD03_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG4302554.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="SDD03_000072"
     CDS             complement(83280..84740)
                     /locus_tag="SDD03_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302555.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="SDD03_000073"
     CDS             complement(84730..86025)
                     /locus_tag="SDD03_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4302556.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="SDD03_000074"
     CDS             complement(86357..87034)
                     /locus_tag="SDD03_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG4302557.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="SDD03_000075"
     CDS             complement(87064..88155)
                     /locus_tag="SDD03_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG4302558.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="SDD03_000076"
     CDS             complement(88256..88690)
                     /locus_tag="SDD03_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4302559.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SDD03_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SDD03_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4302560.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="SDD03_000078"
     CDS             complement(90728..92623)
                     /locus_tag="SDD03_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4302561.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="SDD03_000079"
     CDS             complement(92681..94456)
                     /locus_tag="SDD03_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG4302562.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="SDD03_000080"
     CDS             complement(94506..96002)
                     /locus_tag="SDD03_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4302563.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="SDD03_000081"
     CDS             complement(96196..97818)
                     /locus_tag="SDD03_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG4302564.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="SDD03_000082"
     CDS             complement(98267..99580)
                     /locus_tag="SDD03_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG4302565.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="SDD03_000083"
     CDS             complement(99825..100049)
                     /locus_tag="SDD03_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302566.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="SDD03_000084"
     CDS             complement(100069..100491)
                     /locus_tag="SDD03_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG4302567.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100634..101344)
                     /locus_tag="SDD03_000085"
     CDS             complement(100634..101344)
                     /locus_tag="SDD03_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302568.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101362..101859)
                     /locus_tag="SDD03_000086"
     CDS             complement(101362..101859)
                     /locus_tag="SDD03_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4302569.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101896..102348)
                     /locus_tag="SDD03_000087"
     CDS             complement(101896..102348)
                     /locus_tag="SDD03_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4302570.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102483..103088)
                     /locus_tag="SDD03_000088"
     CDS             complement(102483..103088)
                     /locus_tag="SDD03_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4302571.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103538..104158)
                     /locus_tag="SDD03_000089"
     CDS             complement(103538..104158)
                     /locus_tag="SDD03_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4302572.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104160..105029)
                     /locus_tag="SDD03_000090"
     CDS             complement(104160..105029)
                     /locus_tag="SDD03_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG4302573.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105052..105459)
                     /locus_tag="SDD03_000091"
     CDS             complement(105052..105459)
                     /locus_tag="SDD03_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG4302574.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105478..106767)
                     /locus_tag="SDD03_000092"
     CDS             complement(105478..106767)
                     /locus_tag="SDD03_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4302575.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106794..107096)
                     /locus_tag="SDD03_000093"
     CDS             complement(106794..107096)
                     /locus_tag="SDD03_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4302576.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107121..109214)
                     /locus_tag="SDD03_000094"
     CDS             complement(107121..109214)
                     /locus_tag="SDD03_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4302577.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109703..110467)
                     /locus_tag="SDD03_000095"
     CDS             complement(109703..110467)
                     /locus_tag="SDD03_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG4302578.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110578..111447)
                     /gene="mdcB"
                     /locus_tag="SDD03_000096"
     CDS             complement(110578..111447)
                     /gene="mdcB"
                     /locus_tag="SDD03_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG4302579.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111472..112548)
                     /locus_tag="SDD03_000097"
     CDS             complement(111472..112548)
                     /locus_tag="SDD03_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG4302580.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112572..113165)
                     /locus_tag="SDD03_000098"
     CDS             complement(112572..113165)
                     /locus_tag="SDD03_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG4302581.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113169..114845)
                     /gene="mdcD"
                     /locus_tag="SDD03_000099"
     CDS             complement(113169..114845)
                     /gene="mdcD"
                     /locus_tag="SDD03_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG4302582.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114835..115134)
                     /locus_tag="SDD03_000100"
     CDS             complement(114835..115134)
                     /locus_tag="SDD03_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG4302583.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115236..116882)
                     /gene="mdcA"
                     /locus_tag="SDD03_000101"
     CDS             complement(115236..116882)
                     /gene="mdcA"
                     /locus_tag="SDD03_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG4302584.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116889..117800)
                     /locus_tag="SDD03_000102"
     CDS             complement(116889..117800)
                     /locus_tag="SDD03_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG4302585.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117920..118777
                     /locus_tag="SDD03_000103"
     CDS             117920..118777
                     /locus_tag="SDD03_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4302586.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118964..119326)
                     /locus_tag="SDD03_000104"
     CDS             complement(118964..119326)
                     /locus_tag="SDD03_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG4302587.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(119515..119697)
                     /locus_tag="SDD03_000105"
     CDS             complement(119515..119697)
                     /locus_tag="SDD03_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302588.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            119672..121111
                     /locus_tag="SDD03_000106"
     CDS             119672..121111
                     /locus_tag="SDD03_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302589.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121118..123670)
                     /locus_tag="SDD03_000107"
     CDS             complement(121118..123670)
                     /locus_tag="SDD03_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG4302590.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123784..125115)
                     /locus_tag="SDD03_000108"
     CDS             complement(123784..125115)
                     /locus_tag="SDD03_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4302591.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125081..126559)
                     /locus_tag="SDD03_000109"
     CDS             complement(125081..126559)
                     /locus_tag="SDD03_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4302592.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126864..128699)
                     /gene="tdc"
                     /locus_tag="SDD03_000110"
     CDS             complement(126864..128699)
                     /gene="tdc"
                     /locus_tag="SDD03_000110"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG4302593.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            129169..130596
                     /locus_tag="SDD03_000111"
     CDS             129169..130596
                     /locus_tag="SDD03_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302594.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(130688..131053)
                     /locus_tag="SDD03_000112"
     CDS             complement(130688..131053)
                     /locus_tag="SDD03_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG4302595.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(131050..131415)
                     /locus_tag="SDD03_000113"
     CDS             complement(131050..131415)
                     /locus_tag="SDD03_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG4302596.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(131607..131855)
                     /locus_tag="SDD03_000114"
     CDS             complement(131607..131855)
                     /locus_tag="SDD03_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302597.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            131854..131982
                     /locus_tag="SDD03_000115"
     CDS             131854..131982
                     /locus_tag="SDD03_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG4302598.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            132039..133733
                     /locus_tag="SDD03_000116"
     CDS             132039..133733
                     /locus_tag="SDD03_000116"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG4302599.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            133737..133856
                     /locus_tag="SDD03_000117"
                     /pseudo
     CDS             133737..133856
                     /locus_tag="SDD03_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            134384..135517
                     /locus_tag="SDD03_000118"
     CDS             134384..135517
                     /locus_tag="SDD03_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG4302600.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            135548..135868
                     /locus_tag="SDD03_000119"
     CDS             135548..135868
                     /locus_tag="SDD03_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG4302601.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(135897..136361)
                     /locus_tag="SDD03_000120"
     CDS             complement(135897..136361)
                     /locus_tag="SDD03_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4302602.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(136493..137746)
                     /locus_tag="SDD03_000121"
     CDS             complement(136493..137746)
                     /locus_tag="SDD03_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4302603.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(137775..138092)
                     /locus_tag="SDD03_000122"
     CDS             complement(137775..138092)
                     /locus_tag="SDD03_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4302604.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(138118..138438)
                     /locus_tag="SDD03_000123"
     CDS             complement(138118..138438)
                     /locus_tag="SDD03_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4302605.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(138902..140317)
                     /locus_tag="SDD03_000124"
     CDS             complement(138902..140317)
                     /locus_tag="SDD03_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4302606.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(140334..141650)
                     /locus_tag="SDD03_000125"
     CDS             complement(140334..141650)
                     /locus_tag="SDD03_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4302607.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(141800..142498)
                     /locus_tag="SDD03_000126"
     CDS             complement(141800..142498)
                     /locus_tag="SDD03_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG4302608.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(142787..143743)
                     /locus_tag="SDD03_000127"
     CDS             complement(142787..143743)
                     /locus_tag="SDD03_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4302609.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(143856..144689)
                     /gene="msrA"
                     /locus_tag="SDD03_000128"
     CDS             complement(143856..144689)
                     /gene="msrA"
                     /locus_tag="SDD03_000128"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4302610.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(144864..146498)
                     /locus_tag="SDD03_000129"
     CDS             complement(144864..146498)
                     /locus_tag="SDD03_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302611.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(146485..148353)
                     /locus_tag="SDD03_000130"
     CDS             complement(146485..148353)
                     /locus_tag="SDD03_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302612.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            148319..149956
                     /locus_tag="SDD03_000131"
     CDS             148319..149956
                     /locus_tag="SDD03_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302613.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(150101..150841)
                     /gene="truA"
                     /locus_tag="SDD03_000132"
     CDS             complement(150101..150841)
                     /gene="truA"
                     /locus_tag="SDD03_000132"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG4302614.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(151032..151667)
                     /locus_tag="SDD03_000133"
     CDS             complement(151032..151667)
                     /locus_tag="SDD03_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG4302615.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(151814..153055)
                     /gene="sstT"
                     /locus_tag="SDD03_000134"
     CDS             complement(151814..153055)
                     /gene="sstT"
                     /locus_tag="SDD03_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG4302616.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            153253..154251
                     /locus_tag="SDD03_000135"
     CDS             153253..154251
                     /locus_tag="SDD03_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302617.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            154264..156282
                     /locus_tag="SDD03_000136"
     CDS             154264..156282
                     /locus_tag="SDD03_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG4302618.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(156342..157058)
                     /locus_tag="SDD03_000137"
     CDS             complement(156342..157058)
                     /locus_tag="SDD03_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG4302619.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(157285..157827)
                     /locus_tag="SDD03_000138"
     CDS             complement(157285..157827)
                     /locus_tag="SDD03_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG4302620.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(157832..158059)
                     /locus_tag="SDD03_000139"
     CDS             complement(157832..158059)
                     /locus_tag="SDD03_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4302621.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(158138..160018)
                     /locus_tag="SDD03_000140"
     CDS             complement(158138..160018)
                     /locus_tag="SDD03_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG4302622.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(160207..160821)
                     /locus_tag="SDD03_000141"
     CDS             complement(160207..160821)
                     /locus_tag="SDD03_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4302623.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            160963..161523
                     /locus_tag="SDD03_000142"
     CDS             160963..161523
                     /locus_tag="SDD03_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4302624.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(161828..162142)
                     /locus_tag="SDD03_000143"
                     /pseudo
     CDS             complement(161828..162142)
                     /locus_tag="SDD03_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT        48249 a        33447 c        27945 g        52626 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgcaacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaacg gcaatgaatg ccgctcttct
   100621 tatttcgatt cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac
   100681 tagttacgcc aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac
   100741 gtgccgcttg ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg
   100801 tcagcattgt tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat
   100861 catagtagat gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga
   100921 atgattcgcc accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt
   100981 ctccgccgca aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat
   101041 tgattgggaa aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac
   101101 gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt
   101161 tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata
   101221 attgatgata cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata
   101281 agatttcgat ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt
   101341 tcatctgttc acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg
   101401 atcatctccc atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa
   101461 agcgagcgca acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc
   101521 ttttgctttt tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt
   101581 ttttcgtaca tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact
   101641 cgttcctgta tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa
   101701 atcggtttct ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa
   101761 gtaatcagct aaatgctccg gtgaattatc ttttccaaat gtatctgtaa atgtttcgat
   101821 actaatttgt tgtagtaagt ctacatctga aggcgtcatt tttcgaattt cgtatttatt
   101881 cacggcttct tcctttcaat agatcctttt attgcctttt ttgacaaaat tccaatcttc
   101941 gctaacattc ttgcatactt tatttaaaag atattccaat tgttttgctt cttcgtcaga
   102001 aagaccattt aatgcgactg cattggagta gttgttttct cgaatgatga aaggatagat
   102061 ttctgctcct tttttagtcg gatagagttt cttgattttt ttattttctt tatcttccaa
   102121 acgttcgatc atcccattgg attctaattt cttgattgct cgggcagtcg ttgtacgatc
   102181 tactttgatc atttctgcta gtttttcttg gatgattcca ggattttcgc aaatacgtac
   102241 taaataaaga tactgtcctc ttgttaaatc gacttctttg aattcgatat tgctaataga
   102301 atctagcgct cgggcaatca ttccgatgtc tcgaaggatt tcagccattt catcccacct
   102361 ttattttgtt tgtaagacta gtttacctct ttttgttgca aatgcaacta ttttcaataa
   102421 aaaaatcaag ttctttttta ataactagta aagtatattc ttttcttctt ttacgaaaaa
   102481 gctcaatcac aatagatctg gtttttgttt tctaaaataa gtaactgttt cttcgttgtt
   102541 cgcttttctg atagctttgg caactcttct atactaaaaa atcccatgtt ggaagtttca
   102601 ttattctcaa taaacttggc ctcttcaccg tggatcgcac aagcaaaaac aagtttataa
   102661 tactgaaaaa gctgtggaat atctttttgt ttattcgtat caaaaactgc tcgcaactga
   102721 gtagtttcta ctgttaatcc tgtttcttca tagacttctt tacgcacatt ttcttcagga
   102781 gacaatccta tttcagcaaa tccaccagga agtgaccatt ctttcgtccg tttatcttcg
   102841 attagtagga ccttgccatc ttttttgata taggcacgaa catctacttt tggtgttgga
   102901 tgtccttctc caatcgttgt gagcgctttt atttcttcga aagtttcttt tcctatttca
   102961 ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac gctctttatc aaatacatct
   103021 tttccataat atagaccagc ttgagaaagt gcgagtagtc ttttgtattg agccaacttc
   103081 ttgttcattc tcttcccttc ttttttagca aatgtatgac tatgtaatca agtatagaag
   103141 actggtttaa ttacggcaaa taaatataat gaaatctgca ataaataaaa gaatgttaaa
   103201 aaactcgaga acaaaagaca ttcgacttct ctggggctgt gaataaatgg cgttcaaaag
   103261 cttgcatctg cggtaataag ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg
   103321 ctgttcttat taattgaggc aggacagctt tttcacaacc tccccactaa acggtgttct
   103381 cctaccaact cctcggtcac aagtcacaat attcatcaaa catgagaagc tgtttttttg
   103441 aaattgctcc ttgtgactcg gagctgaacg acaggctcac gacctctcgg taaacagcgt
   103501 tcaaaagcca atttttcgat attaagccat ttctttgtta cttattgtca ttgatttctt
   103561 tttctaattt atccaatagt ggagtgtaat ccacttgagt atagtctgtt acatcaaatt
   103621 catatagtgt tcctaactgg aatttgtcat attttcggtc ggcgatgatc tttttgaatg
   103681 cttctttcgt aatatgattg gtccctttcg ttcgatcttc caatacgtca ccatagtgat
   103741 aatgatccat gatatagctc aaatgacgat ctgattcacg atcacgtttg taccgacgtt
   103801 gacataacac ttcaaagtct gctgttaggc gaatcgtgat cacctgatat ccgtatctat
   103861 ctgctaattc ttggagtcgt gctttttgct tatcactaaa aggatactcg gacacaatca
   103921 ctttctttcc tgcttccata tacaaatcaa gtgctttgta atagaaagga taaactttcg
   103981 tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc gatggattct gcatagtatt
   104041 ccttgatttc atcgagcgtg atggcataca tatctggaaa tcgttcccgt agtttattca
   104101 taagatatgt ttttcctgta ccaggactac cagccagaag aatgaaatac tttttcatct
   104161 catttatttc ctaagttaat tggactttcc aagttcataa acagtccaat cttatgcttg
   104221 acaatttctt ttactgcttc atttgctgga atcacattat ggcgaagaga cttgttgatt
   104281 tctgtttcac taaaggtttc tttagctact ttcgtccagt tgactaaaag ctccgttcca
   104341 acattgaatt tgcggatccc gttattgatc aattcaggat aatcttcttc tttcacacct
   104401 gttcctccat ggataactag ggggacatca actactgcat cgatttcttt cagcaaggga
   104461 atgtttactt ctgtttttga tttgaattgt ccatgattcg taccgatagc aatagctaat
   104521 gcatccacgc ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt atataccttg
   104581 tcattttcag cgacatggat tccttcttct gtaccgccga ttgttccaag ttctccttct
   104641 acggaaacac cgtgcgcatg ggcatattct actactgctt ttgtcttcag gatattttct
   104701 ttgaaaggca gatgggaccc atcatacatg acagaagtat atccgctgtc gattgcttct
   104761 ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac catttcttct
   104821 tctgcaatag ctgtcattac attgactaac acatgcatgc cgatatattt agcggtatcg
   104881 acacttgttt gaataatgat cggcgaaccc atttcttttg ctgcgcctag catagcagga
   104941 agcatttcta ggttatgcgt attaaaagct ccaactgtaa aattcaactc ttctgctgtt
   105001 tttgttactt ctttcaatgt tgtatacatc ttttattgcc accttatctt tttatttgtt
   105061 tcctttgatc gttacttgtt ttgcaatttg tcgcatctca tccattttgc ccatgtagta
   105121 atcgatccct tgatcatcac cagcacgagc agcatctgca cgcttctggt tatagaggct
   105181 catcagtttt ttatacccat ctccgatcgt ctgtttattt tccaaactct tttctaaata
   105241 ttggatggct tgatcgttca catacaattc agaaaattta agtccaactt gttcataaag
   105301 tttcgctaat ttatgcttat cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc
   105361 tgattgaatc ttttcggtcg cttctggcga tagggctttt tcttcttttg ccagaacttt
   105421 tttcggttct ttttcttttt tcttaaaaaa tccaaacatc attttcccta ctttcaatta
   105481 aaatgctttc aaaactgggc ttaagatcca ggcatataag agtgctgcgg ctactgtatc
   105541 tacatcggtt gctgtagcat tgatgaatcc catcgaatta aagatcgtta ctagcaaagc
   105601 gggcaataat gtaataaaga aaccatgagc aataccgcca atgatcgctc ctcggcgtcc
   105661 gccaactgcg ttgccaaata tccctgctgt ccctccggca aagaaattgg ttagcatacc
   105721 aggtaaaatc atcgctaagc cgaacatggg caaagtaaac atggcaataa tcgtaccaat
   105781 cgttgtcgta atgaaaccta aaatgacagc atttgggcta taaggaaaga aaactggaca
   105841 atctaaggca gggatcgcat caggaactaa tttcatagct atccctcgaa atgccggcac
   105901 gatttcacct aacagtagac gtacaccagc tagcaataca tacacaccca ctacgaattg
   105961 gatggcttgt aaaaaggcaa acatgatata gttttgatct cctgataatt cagaagcgaa
   106021 tggttcgcct gcaaataaaa cagtgatgat atatagcggg accatcacga ccatgacaga
   106081 caaataagta tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt cgatcgactt
   106141 tttattttcg cctttctcac caaaaagctt agcaacaccg gcttcaaata agtacccgat
   106201 cgtacaaaaa tgacctaaag caatatcatt tgatccagtg attttacgaa tgatcggttg
   106261 cgctactgcc ggcatagcaa tggcgaaaca agctccgata aaaccaccga ccaagatcaa
   106321 tacgatccct cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg ccatccataa
   106381 aattgcttga ccagttaaaa aaatgtactt ccattttgtg aaacgagcca aaataatatt
   106441 gaatacaaaa atggctaaga acgtcagtgc aatatctcta cctaaaccca aatcattcat
   106501 tgcttgacca ttgatagatt caatagaagg gatgatccct tgcatatgaa atccttcagt
   106561 aaagattttc ccgaaataag ttaaacttcc aacgatgatg ctcgaaccgg cactcaacac
   106621 ttgaaatcct aggagtgtct ttaacgtgcc agagatgact tgtcctgctg atttcttctg
   106681 cagtagtaga cccaacatag caatcaatgc aatggtgatc gatgcctgag tcaaaatatt
   106741 ttcgatgata aaattgataa cgcccatgtt tttaccctct tttctttttg tatttagata
   106801 actcccttat cttgcagaac tggggtgatt ttttcttcaa tttcattttt tgaaacaata
   106861 tttttcaaat agatcatcgc cgttttagga tcaactgcga acttttcgaa ctgcgaacga
   106921 aaattttctg ctgtgatgat cacatctggt ttcatggaaa ctgcggaaga aatatcacaa
   106981 tggtccagtt tggcttctac tcctaatttg ttcaaaacat cctcagcaga catctgtgct
   107041 gcaaaactac ttcctaaacc tgcaccgcat acgaataaaa tgttcaattt gttcatcttt
   107101 gttctccccc gtatcattta ttatttctca actactgaaa aaagcagttg tttgaaatct
   107161 tcgatatttg tgcattcaat caatcgatcc aacttctctt cgttgttgat caattcaatt
   107221 agttctttca tgatgttcag atgcgagtag gagtctactg ctgccaaaca aaaaataatc
   107281 tttactggat ccatttcagg attaccgaaa tcgattggtt gctcaatcgt agctacgctg
   107341 actcctaatt tattgacccc atcttctggt cgggcatggg ctagtgccat gtgtttgcca
   107401 atcacgatat acggtccgta atcattcact gcacgaatca tcgcttcgac atagcgctct
   107461 tcgacaatgt tttctttaac caatggtcta gcagctagtt caatcgattc ctcccaagta
   107521 gtggcttttt ctttgatcat gatattgctg tccgtcaaaa tatcttttaa cataggctgg
   107581 atctcccttt tgttgatttc taaattgttt gtttcgaata atttttctaa cttgccatag
   107641 atgattcgat ctactcggcc accactttct ttcatgacat ctattatttg agtgaataac
   107701 tccgtcgatt catgactgtt aggctctaac cgctgtagtt cttcatttct tttcaaaaag
   107761 tcttctataa ccaagatatt cgtctcagtt aaaatcggat cgacaactaa aagcggctta
   107821 tttcgataat tcaattgaaa ggtgctgaaa accagattga catccaattt atctactaat
   107881 tcgatttctt ttgaacttaa aactgcagta atctcgatct gaggaaactt ttctttcaga
   107941 ttctctgcta acagatttcc agtagccatc ccatggttac agacaacgac tgatttataa
   108001 acataattca agtctcgctt gattacactg gcgatcgtcg aaaagtggat gacaagaaaa
   108061 gctatctcgt cctccagtat cttgccacca attacttctt cgatcgtagg aacaaaatga
   108121 gtgactgcgc ggtagattgg gaaataattt gttcggatgt tttctttcaa tggattcatg
   108181 acttggatat gattcttgat tcttacaatc agtgcagcca tgtgcttata gagattttcg
   108241 aataaagctt cttcctttag atggaaaggt acgtgtgtct cgttctctac aaattggatc
   108301 aattggatcg ttagtaattg agcttgtacc cactcgattg aattggagat atctctagca
   108361 ttgaacgtct caatagtgaa acgaagataa tttctttcta cttgcgggac attgtccaat
   108421 tgaaattcct cgatcatgtg gtcaataaat gcagaaaagc tactgtcttt tatttctgta
   108481 tttttccaat taatggcaga gataaattca tgtttcctga ttcgctggat ccagatcaat
   108541 gtaaaaagca ggatttgttt tttataaata tcatcttctt gtttcaacaa cttattggaa
   108601 tagatctcct gtattttttt gatttccacc aaaggaaaat aatggagaaa gattttttga
   108661 gccggtgtta attgtgcatc ttgattcata aagaagtcgg tcattcccag attctgattg
   108721 ataaaatcat agatcatcgt acggatagat cgttctgaac ctttataaac cagtccttgc
   108781 tttccataac tgacgatctc tatcccataa ctgaccagtg ttgctcgtaa ccgtctcata
   108841 tcttcatcta atgtactttt agagatttga aattcttctt cttttttgtt tagatggatc
   108901 gggattcggc tgtaagcaac ggctaaaact agatcaaaaa tacgttcgcg ccgattcaaa
   108961 aagccgtctt tcacatcttt tatttcagtt tctaatcgag cttgcttttc cttttccagc
   109021 ttcaacaaaa aaactttgtt gcgaattgta cggatctccg gaagcttttt actgctcaac
   109081 cagatattcg cttcaagaac ttcctgacgg atcgtccgct gactgacatt gaactcactt
   109141 gctagatgtt ttgcttttac tggatattgg gctgtcatga ttctgtgaag tatttcaatt
   109201 gttcttgttt tcatagaaat gatctccttc tgatttcaag tatatggttc cgcttacata
   109261 ataaaaagta gatatttctg ccacttgaag tggaaagaaa gtaagcaaag caaacaaaaa
   109321 ataagccata tgctttttca tctcctcaac gaaaaagaca tacgcctatt attacaaaaa
   109381 ttttgcccat ctatacaaaa aagaggttgt gaaaaagctg tcctgcatca agtaataaga
   109441 acaactaaac taaaatagtg cttcctattt ttcgtttggt tgttcttatt atcgcagatg
   109501 caagctttta aacaccattt agtggagagg ttgtgacaga gatgttcagc cttgagcgat
   109561 aagaagcgat tttgaaaaat cgcttcccat attttacaaa atcgtgcctt attgccgata
   109621 ggctatctct ggcacactgt ttattcagaa aaaggagctg cgacaaggct tttgtcacaa
   109681 ctccgttcat aaatatttcc tgtcaatcca gcataatcgg ctgaataaaa tcggcttgcc
   109741 atcttttttc tttctcagaa aaatcaaaat accatatctg agaaaaatac tgtcgttgtg
   109801 tcttacttcc tgagatcaat cgcccatctt ttcgatcgac tgaataatca cagcatctga
   109861 aatcaattcg gatggcaaaa ctattttcgt ggatctgtcg ataatttcct acttcctgaa
   109921 tagttatttt tttagtatga tttcttacac cgtctgcttt gtttttttgc agaactttct
   109981 gatgttcggc aaacagcttg tctgtataac agcttctggc tttttcagga gtttcttctt
   110041 cccaagcgga ttggatcttc atgaatatct tttggatttc cagaaactgt gctttctttt
   110101 ctttagccgt tcctgacatg cgggacaata tctccgcttt cagccttttc ttttgtcttt
   110161 tattcttgac gatagaaaaa gcagaaaccc ctcctactga caaaacagct aatgcaagaa
   110221 atcccgtttg ctcataacta gaaggacctg tacctggacc ataataccca taagaatctc
   110281 tgccgtaaga acgactgcca aaataactgc ccccatatga acgaccacca aatgaacctc
   110341 tgcccgaact tcgaccgcta gatgtacctc caccatgacc acccgcgaca gcgaatcctt
   110401 gggtcggcag agcaaacaaa cataggccca ctaacaaaat gattgctaaa taattgattt
   110461 ttttcattgt cttcctcgtt atcttttatt acaaaaaact atttttatga tagccttgct
   110521 aaaaggaaga gtcaataacc agatgcataa actgtttttt tagcaatttt gtcttattta
   110581 gtcattgagt aacatcggac aaatgtatgt gtcggcagtc cataattcct ctatcttcag
   110641 catgaacaag cttgcagaca agagatctgc gcttcctcca ggagagattt ggttttcctg
   110701 acaaaagtgc aacagtgctg taaatggacg atttggtaaa gcactttgac tagcagcatg
   110761 agcaagtttt tgcatttcca ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc
   110821 atctagcgaa ctcatcaaaa ctaacaacat atgcatttgt ttctgtacat atgaatcatt
   110881 atctagctcc ttagctgctt ggattgcaag cctgatatgc ggatacccta atatcgcttc
   110941 ttcatatgca ccatttacgc catatttttg cttcacccta tgaccatgag tggttttttt
   111001 atgacgatat tttgtatcag gaaaagcagc aagcttgctg acatcatcaa gtattttcga
   111061 taaagaaaaa ggctgttcca tactgatttt ttgtgcaatg acactgatca atagccccat
   111121 tgcccaaatc gctcctttat gggtattgat gccatttgta gcaatcagca ttgccttttc
   111181 cgcttttcgc ccaatctcag caattctttc tcttaactcc tgatcaatag catgatgcca
   111241 tgctatttga gccatttctt caaaaaaagg agttagcgtc ttagcagact taagaaataa
   111301 agcatagtcc atatcggaat gtgcacccgc atccagtgca tccactaatc caggttttgg
   111361 acttaatgtt acttctgcga tcaatgcttc ctctaccaac gcagcgagtt gctgagtcac
   111421 tgcttttttt cgttcgtcat ttatcatgac actcactcct tcttcctact attacttgct
   111481 gaaacctttt ttgatagcta cccaaatgat atgaagtaac cagcttaaag caaatgcaca
   111541 acttgccgtc attagataag ctacaagaat catgactcct gaaggtaact gcagccaact
   111601 atctttaaac aataataaca gtacttggaa aacaaataca ttggctaaat acgtacgata
   111661 agcataaaca gacaaccatt tgaacaatgg caattttggc gaatcgttta caatcatttt
   111721 ggaagcgcct atgaagacag caaagatgat gactaaactg tataaacttt ggatcgtatt
   111781 caagtaaggt gcattcgcaa agctcaagtc tcctggatgt gcgagcaatg aacggacaga
   111841 aaagaacgcg ataactaagg caacaggtaa gaaaagataa cgtactctgt ccaaatattg
   111901 atagactttt tcgtgataaa caaatgcggc taccccataa atcatgaaac tagcaacaaa
   111961 ccgatctaat aagtaccagc tgtcatgata aggcccagtc aatacttgtg tttgatagaa
   112021 cagataccat gcgacatata aaatcgttga accaatcaat actggccata catatttttt
   112081 attagcaaat actttgtaac cgagccaaac aaaaaacggc atgaacaatt ggatctgcag
   112141 catcataacg gtataccata aatgaggcgc tccatcacct gtgacatatt tcagtaaaaa
   112201 gcttgcagta tccgtatacg gagtttgttg ttggactgct ggaaatagcc aaagataagc
   112261 cgtcgtccat agaagatacg gaacgactag ttcaaaaaac ttttctttca aaaattccca
   112321 ataagacaaa tgctgacttg tttttaccat attataaaca attgcaaaaa taaacattgg
   112381 tgctgtgtat ttaacgatga cgtaaaaggt accaagaaaa gctgtatttg ttgcgctgtg
   112441 gctgtttgcc aaagcaaagc tcaatatcgt ttgcatcata acagccgtta cagctgatac
   112501 tttctggaag tcacttacgt tgattttgat ttttttgttt tcaaacatct ctttctctct
   112561 ctttttcttt attaccaaag ttctttggtt aatttaggtc cttcgactgt tttcaataga
   112621 atttttttct ctggctctct caaatactct tttagtgaaa aaccgccttg ccgagtcact
   112681 acttgggtat cgaattgctc aaaaaaagca ggatgcgatt gaattacctg aattggcaat
   112741 tcaatcggtg aatttgcgta cagcagcaaa tcaaaatcac tggtttcttt tactgccgga
   112801 atcccagtcg ataattcaaa tcctaagctg ccgccgattc cccatttaca tcctgataat
   112861 atgtttctag cagcatgaaa ttgctgataa acaggataat ccatgatttt ctcttgtcta
   112921 tacgattctt gttcgatgat gtcccacgga tgaatgactt gtttgacggc atgttttgag
   112981 atttctgttg accatctctg attgcgggca ggtccacgta aaccaatcgg tagtatttct
   113041 gcgtgatccg tcgacgcacg acgaactgtt ccgaacatct tttcagttag aacgacccat
   113101 tcaggcaagg tgaacccttc tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga
   113161 atcatttttt atgcatccca ttcttcgtcc attttggcac ggactttatt tgtttcgaca
   113221 cgtccgaatg ttttggctgt ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta
   113281 ttgcggacat cttgaatggc ttcattgatt gcttcgatga ctttgctgac attttgttct
   113341 gaagcagctt ggtcggtgat atcttctagg aaatgataga tcgctcctag ttttgtataa
   113401 ttattgatat cgtaagcgat agctggaact ttttcagcag ctgcttcgat ttcagcaacc
   113461 gaacgttttg tcacacgtgc agcactttct ttagacatcg cttggacggt aatttcttta
   113521 ttgtttaaag caatcaagcg gcttgattgt aatccatgtg ctaaaaatgc acctgaaata
   113581 gcatttcctg caatcaatcc aataactgga tgtccagctt gacggagttt tgcataagca
   113641 gcagcactat tagccaaagc cacatggata ccgatcaatt cttcgttata gccgtatgct
   113701 tggcttggta catcgatcac aacgatgatt ggccgtttca cttttttgtc tttatcttct
   113761 tcgtaaacgt gatccaaaat ttgtcccatg cggaaacctt caactaaacc aacttcaccg
   113821 tttcgtacac ggtacatcgg gtttttagga tcaggtacga ttgatgtcac gatggcttct
   113881 tctatgaatt tacttgattt accataatac acagtactaa tagaagaagt tggatttttc
   113941 atgccagtta gtgccgcaaa ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct
   114001 tctgctactt ctggtaagtc aattgatttt gcttgatggt tagcaattgc ttcattgtaa
   114061 tcgtcaatgg ccattggttt tgaaagatct aaagaatcaa gtaatgataa ataaaatgct
   114121 gagctttcac tgcgatgcgt gtcttttcgt tcgtccattg ctgcgatgac agcagctttg
   114181 attgcttcta cactatcagt cactaattca tcaatgattt ttgattctac ccgttgacga
   114241 gatcctaatg tattccaaat caagtctttg tcactagaat caaattctcg aacgcctgct
   114301 tcttgttcaa taacttctgg tccattcaaa ccgactctcg ctttttttgt tgcgattaaa
   114361 taactcatta atgctgaagt aatcgacata ccgccaaatg atcctacacg tcctggaatc
   114421 aaaccgatga ctggtacata ttgttttaga gcaatgatta catttccgat ttctgaaata
   114481 gataacagtc cgtagtttgc ttcttgcaaa cgaacaccac ctgtatctag tacaatgact
   114541 ggaaagatct catttccttt tttattttct tctaacacat gttcaagtgc agcaacgatt
   114601 tttgcaccgg aaacttcacc gataccgcca ccttggaatt ttccttccat agcgattact
   114661 actgcttgtt tgccattgat cgtgccacgg ctgacaacga tcccgtcatc actttcaggg
   114721 acgattcctt gtgctggtaa acttggcgcg atgatttgat cgaatggatc aatcaattca
   114781 cgtccattgt caagtagagc gttcactcgt tctctggcgt ttaattctac aaaactattt
   114841 ttcatctgaa agttcctcca ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc
   114901 accgaagtca ttgatcacga tatctgctga aattggataa cgatcaaaaa atctcgttaa
   114961 tacattgttc catacttcgc cgaagccatc gcttcctgta caaattttta cagtagattc
   115021 tgttttttct gttggaaaca tcaagatttc caaatctcct gaaccgacca ctcctacatg
   115081 gattttctta ttgatcggct gtttcgttgg atatttataa gctaattgtt ccatggatct
   115141 ctcccctttt tgtaaaacag agcatacacg ttctgcttat tcgtctttct tcttccatct
   115201 gggtaaatgg cgacaaaagg tttttctcgc caattttatg accaagttct gaatttcatt
   115261 ggtggttcat acaggccatc tgaccattcg atcaaatcat cgatggtttg tgcagctaat
   115321 aatgaacgtt tcgcttcact acgtttgatg cctagatctt ctggtaattt cacgatgccg
   115381 gctgcacgca agctatctag tgattctttc gtgctagtca ttccaagagg tgtaacaccg
   115441 ccaattgctg caattgctgc ttgtcgttct tcttttgttt ttgctttgta caaataagca
   115501 attccttctt ctgtcacgat atgtgtagta tcttcgctat aaatcatcac tggtgcattg
   115561 gctaagcctg cttgtttttg aacagctagt gcatctaatt cttcgacaaa gactggtttt
   115621 ttatttgaac catatgtttc aaccatttga acaactaatt tttggccttt ccctaatgga
   115681 tcatcattgt cgcgaagtga ttgccatgct ggtgtagaat gacgacgacc gccaggatta
   115741 tgtcccatgt taggcgcacc gccaaaacca gataaacgtc cttttgttac ggtagaagag
   115801 tttccttcgt aatctaattg caaggttgaa ccgataaaca tatcgatggc atattgtcct
   115861 gcagcttgtg ataatgttcg gttactgcgc atcgtgccgt ctttaccgac aaaaaagaca
   115921 tctgggcgag cagcaatata tttttccata ccaacttcgc caccaaaact atggatgctt
   115981 tcaacccaac ctgattcgat ggctggaatc atcgttggat gtgggttcaa tacccagttc
   116041 ttcgcgattt tcccttttaa ccctagtgat tcaccataag ttggcagtaa caattcgata
   116101 gctgctgtat tgaatccaat accgtgattc aaagattgta cgccgtgttc tgcgtagatt
   116161 cctttgattg ccatcattgc cattagaatt tgcaattctg tgatgttttg aggatctcgt
   116221 gtgaataaag cttctaattg ataaggttta tctgctttca cgacaacgtc tacccagtca
   116281 ccagggatat ctacacgtgg taaagaatcg acaacttcgt tgacttgaac gatcacgata
   116341 ccatctttga aagctgcagc ttcgatgatt gttggtgttt cttccgtatt gaaacctgtg
   116401 tacaagttgc cgtctttatc tgctttatct gcagctacta aaacaacatt tggaatcaaa
   116461 tcgatgaata agcgtccata aagttccaaa taagtatgga tatcacctaa tttcattgtg
   116521 ccgtcttcaa tcatttgggc gacacgcaag ctttgtgcac cagcataaga gaaatcgatc
   116581 ttgcttgcga ttcctttttc aaaaatattc aaatgttctg gtcgtgagat actagacatg
   116641 atcaaatgca gatcattcac tttttctgca tctacttttt caagactttc tgaaagaaaa
   116701 cttgcttgtt tttgattgtc gccttctaat acaactttgt cgccgggagc aatcagtttt
   116761 tccattactt ctacgatttt ttcagtttct acatacttgc cattgagcaa gttgctgatt
   116821 ttttccaatc gctgcgcttt tttttcgcgt ttagaagccc agttgcggtt tggtttttcc
   116881 ataattagtc atctccattt ttgatataga aacgtggcgt catcaatacc atgttggtct
   116941 agattgatcg tacggatccc tttatctaca ccaaattttg tatgaatcag ctttgtcaaa
   117001 gtattgccgg ggggaaattc aatcgtcaga tccattccta attcttttgc caagtccatc
   117061 atttggttcc actgcacagg gaaagctagg ttttccaaca aatctttacg aatctcttct
   117121 gcgtcctgag tggctcttcc atccgtattt tttatataca aacatctagc atcagataga
   117181 tggacagact gcatatacgg cttgaatttc ttgaccgtat cattcatcaa gagacagtga
   117241 gatggcacag ggactttcag cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc
   117301 aacgtaacgg ataaaccttc gatactccca gataacgtat gttgcatcgg acaattttca
   117361 tttgacagat aaactggatg gtcaggatca aatgtttttg ctacaatttc ctcagcttcg
   117421 cttctcgtca cacctactac tacgcccatt gcataaccgc ttggataagc ttctttcatt
   117481 aagactgcgc ggtgatagac tagttccgct gcattctcaa atctcaaaga tcgacaagca
   117541 acagctgcac aaaaagcacc aatcgaatgt cctgctgcaa tatcaggctg aataccgttt
   117601 ttttccattt catctaaata aaatactgct tttgtcagta atgctagctg gataaaaacc
   117661 gtatcttgat agttttcttc tttatcaact aaccgatacc ccgtcacatt ttctaaacgt
   117721 tccatgtaca tttcagggat atccgctaac atccctaact tctgactgcc ttgtccagga
   117781 aataagaaac ctgtactcat gagcctcgcc tcctcagcac ttactttttc cactgctatc
   117841 ttagtcaatt tctgctataa taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt
   117901 atggatggga tggataatta tgaaattgct tcatctgcag tattttaaaa aagtattcga
   117961 aacaaaaaat gttacccaag cagccaaaga attatttatt tctcaacctg ccttgagccg
   118021 tgcgatcaaa catttggaaa acgaattagg tgtccctttg ttttaccata gcggcagaaa
   118081 cattgagacg accgtttatg cagaagaatt ttatccatat gcagtcaaga cattagacac
   118141 attagaagaa ggaatcaaat tattgtcttc ggtcaatgag aagatcgtta cttccgtcat
   118201 attgtattta gaggtagcct ctgtttctat tccgaacttg gtccgaattt ttatggaaaa
   118261 gcatcctgat atccaattaa cgatcatgca acatcattta ccagatgata ttaccgatcc
   118321 tgtactgtac atcacttcgg agatgaagcc tggcatgacc aatattccta tcatgaaaga
   118381 acctgtttat gtcgccattc ctaagaaaca tcctcttgct aaaaaagaac atctgcaatt
   118441 agaagatatc caacgcaccc ccatcttgat gctgtcaaag agcaacgcct tccgtcatac
   118501 actggatgca gcacttgaag cgaaaagtat cgaattgacg atcagttcga cgacagatga
   118561 tcctgcgaca ttacgttcta ttgtcaagca aggattagct gttagctttt tcccaaaagt
   118621 ttcttggtct tacgataaaa atgatccttt cgtcttgcgg gaaatcaaag atttgccctt
   118681 gacacgaaca atttatttat cttctagctt cacagaagat catcctttga tcaaaatgat
   118741 tgcaaaaacg atgcgggaat tttatttagg aaaataaaaa aaaaagagct gggacaaaat
   118801 tcttgtcgca gctctttttt cgaacaaacg gtgttccaga agtaatttct tagaaaataa
   118861 gccgaaattt cccaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
   118921 ctaaacattt ctgtcacaac ctcttctcta ttgtcctaga cattcattgc gttgatttaa
   118981 aacttccatc aatcaaatct gctagttcat ctagcttttc tattttacgt agatcaatag
   119041 agatccaatg ttctttattc atgtgatagg ctggaaaata cccctctttt cctcgcaaaa
   119101 ttccgccaag ttcaggatcg accttcaaat caagaatatt gatgatccta tggtcacttg
   119161 catcatcata gattttttct tctggaacag acatcaggac cccaaaccat tttttgcttt
   119221 tttgatgtct gaatacgcaa taatcaggaa attttttaaa aatatattct ggagacaaat
   119281 tgtatgtttc attgatgtga tcgattagtt cttttttcgt tagcatctgc ttccatctct
   119341 ttcttattga ttcgctttat tcaaagatac cacgtaagaa tagaaaaatg aatacaagta
   119401 gttgctaatc aaatacttcg atatttttct tttttatcaa ctcttaaatg ttcaatccgc
   119461 ctgtgttggt cttgctacca tctactatta aaatgtttat tattgtttgc ccacctattt
   119521 tttgatttta gtggtatcat ttttcaagtt catgatgaaa ttcttatcat cagtaaatgc
   119581 tccagaaaaa taaacaatga ttgaaaaatc agttgaaact tgaccaccgt ttattttctc
   119641 cgtattctta gaatagaagg gagtctgatt gatgtattca ctaatgaaaa aaatcattac
   119701 cgaaaaagat attcgacgtc atgtttcttt agtagaacaa ttgctgaatc atacaaagat
   119761 aaccgtaaat gaattagctg aaatcatagg gacaaccgaa cgaacgattt tttctgacct
   119821 tcaatctatt cgctctcagc tgcccgaagg ctgggacatc atcagcgatc aagcaggaat
   119881 cagtttgcaa aaccagcaaa atttattaac aaatgacctt tgggagattt ttttcaaaca
   119941 atcagtcagt gtcgaactgc tgaaaaatct gctttttaca aaaaaagttg ctgtccctga
   120001 ttttctagcc gaccatgggc tttcttatgg gacattgaaa agacatgtga ctaaaatcaa
   120061 tcagcgttta gcaagttacg acttgcagat cgatttgacg aaatatacag cttgtattct
   120121 tggaaaagag cgtgcgattc gtacttttta tcatcgattg ctgattcctt tcactcataa
   120181 caactatttt ttcgaggatt attcgatcca tgaatcccat tattttcaat ttttaagaaa
   120241 cctcagtcaa actgaactag cggttgaaac agaagagatt ttcggaacat gttggttctt
   120301 tatcaatacg atccgtataa aagcgaattg ccgattagat tcttctatcc ataccaacag
   120361 cacactctcc tccctgtatg actctgcatt gaaaaaattg tatttaacgg aaggtattta
   120421 tcttaaagac actgagcttt cttttgcttc tttctgcttt ctggaaagtt ggaattacaa
   120481 caataactat ggacaagaaa ccgctcgctg cctgcatcat tctccatttt tagaggtcct
   120541 cgaaacgttt gtagaggaac tagcttcaga gctgtctctt gatcaactaa aaaaaacatc
   120601 tctgactgat aacttggctc tcctcatatt aaaatatcat gaatcgccga tattgattga
   120661 agcattggac cgtcaatacc atcgtttttt agaaacatat gatcagcaat atgcacactt
   120721 gtatcaacag aaagagaatt tactgaacga tctaaaaaaa gaaatagaaa tcaatgaacc
   120781 aaattacttt ctccaactct tgtctctact gatccaacaa acgatttttt ctatcaaacc
   120841 gaatttgttc aatgtctatt ttattttcca aggagaacct tcatggaagg cgttcttgca
   120901 gcaggaatta aaagattact taggaaaacg tgtctgcttc ttacctatcg aactaaccga
   120961 tctttccact atttcttttg aaaaaacaga tatcttgatt tccaatttcc ctctagattc
   121021 tctagatatc cctattgtat acatttcttc tattccaacg aaaaatgagc tcaatcgttt
   121081 gactgaactc actttcaaaa cttttttata gtaacggcta gcgactgtat tctctagctg
   121141 ttttttcttt tttgcgaaac agtttgttca tgatcccttt ttctatttct acaactaaga
   121201 atagaagaat cacatttgct ccaatcaata actgttgtgt catcgttaaa cttactgtac
   121261 cgatcagctg ctggccgact ggtagaaaaa taaccatccc ttgcaataaa gcaaggatac
   121321 ctaatgaaag aaaaagcgct ttgttttgca aaagaccttg gttcaatgac ggcttggaca
   121381 actctctgca attgatcatg taaacagctt gagctaagac gatattttgc agaagaatgg
   121441 tttgctgtaa gctttgccct tcaaactgca tagccaataa ataggcagga accatgatca
   121501 gtagggagac atagatgatc cgaaacaaat gataaccaga taggattcct tgctttggat
   121561 ctcttggtgg gcgcttcatg atttctggat ctggcgactc gaaccctagt gcataagata
   121621 aagtgattgt cgttaccata ttgacccata aaatctgtac tggggacaaa ggtagcgggc
   121681 gattcagcat aagtgcccaa atcaagatca atccttgagc taatgcagtt ggcaagaaga
   121741 aattgatggt tttttgcaaa ttatccaaaa tccgccggcc ctcttttacg gcttttgcaa
   121801 tcgtatggaa attatcatct cctaaaacca tatcagctgc ctgtttgctt acttcactac
   121861 ctttgattcc catagcaatt cctacatcgg atcttttcaa agcaggtgca tcatttacgc
   121921 cgtctccggt cattccgacg atttctccat ttttctgcaa agctttaaca attcgcagtt
   121981 tatgttctgg tgtcgttcga gcaaagacat cgactattgg cacttgctga atcaactctt
   122041 catcagacat aagatccaaa tcgatccctt ctaaaacttt ggcagtatgt tttaagccaa
   122101 tttgttccgc aatcgctttt gctgtgtctt tgtgatcccc tgtaatcatt ttcactgaga
   122161 tccctgcttg ttggcattcc tttacagctt tgactgcact ttcttttggc ggatcgatca
   122221 atccggcgat ccctgcaaaa gttaattggg ttaaagtctc atgcgtgagt tcctgtttcg
   122281 aatctacttt tttataagca aatcctaaaa cacgctgacc ttttctcgca aattctgcag
   122341 ctgtctgact ccatgcttct tgttctgcag aagaaagagt tgatttagca aataatactt
   122401 ctggtgcacc tttgacgtaa atcaccgctc ctttttcctc cgcatgcatc gttgccatat
   122461 atttgtattc agaactgaac ggaatctttg cttctatttt tttcaaaggt agattggctg
   122521 tttctgtgtg tcgaatcagc gcaacttctg tcggattgcc acttaaattt tctgttttct
   122581 gttcttcttt tgtttttaat tcctgacagt ttttcataat ggacaacacg caggcttctt
   122641 cttttgcagc tacatccaca actgtcattt cattttttgt caatgttcct gtcttatctg
   122701 aacagatcac tgtcatagaa cctaatgttt ctaccgaagg aagtcctttg atgatcgcct
   122761 tttcttttgc catttcatgt actccaacgg atagaatcat ggtcaaaaca gctggcaatc
   122821 cttcgggtac cattgacacc accaaagcaa tcgtagccga aagaaggata tgccaatcca
   122881 taccatatcg aaatgtcgta aaaataacta agaataggct caaacaaaga atgccttgaa
   122941 aaatttgttt gtttaatcgg ttgatttttt taatcagagg cgtcgtttgt tgttcgaccg
   123001 attgcaatgc ttgattgatt ttaccgattt ctgtcgtgtt tcctgtttct acaacgacac
   123061 cgactgcaga accagattgt acaagcgttc ctgaaaaagc catatttttc tggtcgccga
   123121 tcgattcttc ttctgtcaaa acggcatgac ttttttcaga tgcttctgat tctcccgtca
   123181 aaatcgattc ttccactaat aaatcatgtg cttcgattaa tcggatatct gctggaacga
   123241 catctccggc acgtaagaca atgacatctc cttttaccag ttctttcgca gggatttttt
   123301 cttgcatccc gtttgatagt actatcgctt cttgtcccat cagctgcttc aaaccattca
   123361 atgattcttc cgcttttctt tcttgccagt aactaacgag tccattgacg atcactacga
   123421 ataaaataat acttccttca atatattcac tagaagcaaa tttcaaaaaa gaggcaatga
   123481 tcaacacgat catcagcaga tcagtaaaat gctttgctat ttttttccat cgttttgtct
   123541 gctgccttgc ttttatttca ttatatcctt gatcagtcag gcgtttctgt cgttcttctt
   123601 gcgacaagcc ttctgttgat gtttgatagt ttttcaatac atcttctacc gtcaatctgt
   123661 accagttcat cctatcccct cctgtttaat tgatactgtt attatacgca gggaagtttt
   123721 ttcaggcgta tcgattattt caggggggtc tgaaataatt cgttttgcaa aatgtatctg
   123781 ggcctaactg atatttctat tgcttattct tttatataca aaatttaaga atacaaacca
   123841 gataaccaat aaaaaaggaa taaagcgtgt cgtgctttcg aataacaagc tgaaagcaat
   123901 tgaagtaaaa aacaccaaca aagcaagatc tttatatctt tgtgtagtgt tccgtttctt
   123961 ttctttaata aattgaacat gtgcacacaa gatcgaagac cacacaaata gaaaacaact
   124021 cgttgtcatg cctgccaaaa gctgaaaaaa caatcgacta tcagctacaa aaaaatgtag
   124081 acaaagaccg gcaaaaaata acattcctgt aattaaaata ccgtgtaaag gtacgccttt
   124141 cttagaaagc gcacaaaacc aatggggcaa acttttttct tttgctgttt gatataaaat
   124201 cctgctagta ctgtagactg cactatttgc tgaggacaaa gaagcaacca aaatcattag
   124261 attgacaatt gctgctccgc cttggcaatg gatcacttgc agcatagtca ctaaaggact
   124321 ttcttttaaa tctaaacaat gccatggaac aacaaataaa atcgacaaaa tcgttcctat
   124381 atagaaaaag agaatacgcc aaggcagaca gttgattgct tttttcaaat tcttttgcgg
   124441 atcacttgtc tcgccaacgg ttaagccgat catttcaatt ccagcaaaac tgaataaaac
   124501 cataggaaat gagactacca ttccagcaaa gccaaacggt agtaattcat cgatctgtcc
   124561 aaaattttct ttgattccga tagactgggt atgaaaatct gagacactca atcctatacc
   124621 caataagata aaaaggaaaa tccccaagat cttgatgatt gagaaaaaag attccaattc
   124681 accaaaccac ttgactgcaa caaggttgac tgccattaag atcattagtg cgctacctcc
   124741 gcttatcaaa gctggtacat gtacaaacca gaaatggata tacaatccta ttgctgttaa
   124801 ttccgcaagt ccagctccaa gccagcaaag ccaataagtc caattgacaa aaaatgccca
   124861 ctttttgcct aaataatagt caacgaaatc actgatagag ttaaatgtac tatttgctaa
   124921 aaataaatca cccatggccc gcatcaaacc ataaatagcc aaaccgcata tcaaataaac
   124981 gagaataaca gctggccctg ctgatgcaat tgcctttgct gaacctaaaa ataatcctgt
   125041 tccaattgcg ccacctaatg cgattaactg gatatttcga ttagttagag tccgtttcat
   125101 tgccggtttt tccatcaagt tcaccttttt ctttttcaag cagcatggcg acttcctgtt
   125161 cccaagaagg tttctttatt ttttcaatac agagtggtac tacaaacaaa atgacactga
   125221 tggctgtgac tgcaattaga ttgatccatg ttccagcttt catcgtcagt ccgacagaaa
   125281 atccgattgc cgcaaataaa acaataacga cagtccaaac tccaagattg ctctttttct
   125341 ctttttttcc aattctaaat ggtcgattca actttgcatg tgtataacgc aatctcaaaa
   125401 ctgcgatccc catgatgaca tagacgatac agtagataac agtagttgaa tttacgagca
   125461 tcaaaaatgc ttgattgacg ccaggaatca gcaaataaaa aagtgcaaag attgagataa
   125521 tgacagactg agtcaaaata acggatttgg atacgtcaaa ttgattcgtc ttccaaaaat
   125581 tgaattttgg tggatacgca ccttttctag cactttctgt cactgtcttt gctggtcctg
   125641 aggcccaagc agataattgg actgctacac caatgaagac aagtaaacta aacagattca
   125701 caataagatg tggcaaacca agaatttggc aataaatcga aatagattgc gcgatattat
   125761 tcaattccat ttgtcctttt ggtacgacat ttgctacaac taaagcattc aaaatattga
   125821 cgaaaaatat aaaaattaac gcggcaaata ctccgcgaat atacgtttta actggatttt
   125881 ccaatcgagt gatatacaca gaggacattt cgataccagt aaaaataaac atgatcggcg
   125941 caaaataaac aaatgaggat gcagtcgtag tatctggtat taatttgtcc catgaaaaac
   126001 tgcctaatgt tccggttggt acgatcccaa ctttgatcca cgcagcaaac cctaataaga
   126061 gcatcatcgt caaaggcacg tacaatccta accaaacacc gacttttccg ccgatttttg
   126121 ccatatcaaa tttcaaattc aaaagagtga tgatccaata aacgactaaa atgacaacta
   126181 atgtaaattt gctgttcaaa ccaagcggca cattatcgat aatattacca aacaacggag
   126241 cgagtgctga agcgaccatc accatcccag gaaacatttg gacccataac agccatgaga
   126301 caacgaaccc ccattttttt cctagagcat tcgataccca aagttctgga ccaccttttt
   126361 ggggaaacat tccagcaaat tcaccagaga tcaatgagat tggaaaagcg tacaataata
   126421 ctgcaaccag catgtaaaaa atcatcgtcc agctcgttgc tgctacatct ggaatatttc
   126481 gtacacttgc tacaagggcg caggtcattc cgataaaggt catggtcgtg atttgctttg
   126541 atgaaccagt atctttcatc tttattcctt ctttcaatag cgacatatgt ttttgttttc
   126601 ttttattttt gttcaagaac agaaatgaca aggtttcatc gattcagtac aaaacccagt
   126661 atcaccgaaa catccattga acgatcttag tgaactgggt ttgatacatg aaacgaacga
   126721 tccaacttat agaacgtttt ataaaaaaga atatcttggc gaggaatata tgaaaaacag
   126781 ggggaaatac aattgttttg atgaagcaaa taattgtctg ttctccatat gtctcttgtt
   126841 attctttcac acaaaaatca gttctattgg atgatttcgt ttagtttctt ttccattgct
   126901 ttggcgattt tttttgtata aaaattgaaa attcgatcat cattcaaata aggagtcatg
   126961 atagccgcac gtagcagagt gattttctgt tcttttttcc attcttcttt cgttagaccc
   127021 attctttcaa caaaacgaat cggagaatca ccgtaatctt cttgtgtaaa tgtagtatga
   127081 gaagtaataa accgttcacc ataaacatcg ccatccatat aagaagaccg atcgaacatt
   127141 ttttcattta attcattgat agcgttcaaa tctgtacagc cttgttcttt gaatacccaa
   127201 ttgaccatat taaaatcagg atgattcaat ggatagactt cgattgtttt tcctttaaca
   127261 gtaaaagtca gatgatcgag aaattctctg aatctttgag ctgcctcgat tgaagcccca
   127321 atcaattgac catatccggt cacattcaat ggcaatacac gatgtgctgt ccagactgcg
   127381 gcggcggttg ctccagcttt tgatccttcc aaaatataag ctcctaacat atctggagcc
   127441 tttactgatt tttcaaacac atatggcgcg aaataagaaa tgatatttct catattttta
   127501 tgtttgatta cgattcctcc agcagcataa gggacatagc ccatcttatg aggatctatc
   127561 gtgacagaat ccgcttctga aatagctctg aacccttcat atacctcttt gtcaatcgtt
   127621 acgcagtggt ggaaaacatg atgttcttca aagaattcag ctaaggaagc atacggcaca
   127681 aattctcctg cttcgtttag aaacagtgaa cgcgcatagc cgccatatgc ggcatcaaca
   127741 tgtaaataga aatagatccc ttcatctttt aacctttctc ttaattggac gattttatct
   127801 acactgtcta cttgaccttc ttctgtcgtt cctacgacag caaccacacc tagaattggg
   127861 attttttgtc cagctaattc acgaattgtt ttttccaaag cattaatatc cattcgataa
   127921 tcctcttgaa ctgggatagc aaccatctga tctaagccga ctccgcaaat atctaacgcc
   127981 ttcatccaag aatagtgttt tgtttgaggg accagccatt tccctaatct ttggatattt
   128041 tttccgctac gggaagaagc tgctttgacc tcatccattt cttcgtccgt aaagctttcg
   128101 gtcatttcca aaatttcttc aacagataaa ttcaaaagtt cccactcaga catttttttc
   128161 actttttcag gcagcacttc cttgacagct aaaggaatcg atttgatgca acgggcgtac
   128221 cataatcctt caagattagc aatagaacca tctgctgcaa tgtgtcccca tccgtctgtc
   128281 atattgaata atgaagcaaa atcctgtcct acttctgctt ccatttgcga agttgccata
   128341 gaagattcca atgcaacatt atttgggttc cacagcatcg catagttgta agctaataaa
   128401 gcaggcatca atgtttcagc attcatttgt ccccagtaac ggcctgctga atgccatggg
   128461 atcgatcctg cgcgtaatcg ctgactgact tcatccaaga cctctatcat atggtcgcgg
   128521 gtggctagat aatctggagt gagcttatct ccttcgctaa ttgcaggcat atctgaagga
   128581 atataatttt ctctccatcc aagatgttca tctaccatct tgttcaacaa cattttataa
   128641 actggtccat tttcagcttt gtcgcctata aagacggcct tgatatccat atctttcatt
   128701 tttcttccct ccatattttg ttggtcaatg tcttgcagat acatgtgttt atctgtaaca
   128761 cctcttcact atagcgccaa aaatgagaaa ttttttttta taacatcaaa tccacgtaga
   128821 tttttacaaa caaaaataaa taaagtcgga tttttctgat aattcaacgc aaaaaagcga
   128881 acaatttttc aataaatcag tcttctaaaa tagataacct aaaaaaagcg aacaaacaaa
   128941 taaaatgact gtccttcatt cactaaacat atttttattt ttttatattg ttaaaaattc
   129001 acaatttatt ttttaataga attatctgtt ttccaaattt tataacataa aaaacctttt
   129061 ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag aaaacatgtc
   129121 ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat gattgaagaa
   129181 tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta tgtaaataaa
   129241 gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac tttactcaat
   129301 gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca tcgggaaaag
   129361 gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat gcagcaattg
   129421 tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga tgaacctgat
   129481 tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc attaaaaaaa
   129541 caagtattag cttactttaa tgactgcggg atcgaaatcg accgctactc ccctcgattc
   129601 actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat
   129661 tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg
   129721 gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct
   129781 atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa
   129841 atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa
   129901 acctattata aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat
   129961 ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt
   130021 gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa
   130081 ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat
   130141 aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca gacaaattta
   130201 tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg
   130261 ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca
   130321 aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg
   130381 gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt
   130441 atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca
   130501 ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc
   130561 gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca
   130621 cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac acgtcaggct
   130681 cacaacctca cttattttgg acatctaggg gtccgatctc tttatttaaa aaataagaaa
   130741 tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga
   130801 taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc
   130861 ccaacatctt tttaatagca ttgttctcgc ttactcggtc gctttgcact tttgtatgga
   130921 caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga
   130981 tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga aactgcatca
   131041 actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt
   131101 caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt
   131161 ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa
   131221 caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata
   131281 gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat
   131341 taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa
   131401 atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt
   131461 aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa
   131521 atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa
   131581 aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt
   131641 ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt
   131701 ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca
   131761 aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg
   131821 gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc
   131881 atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc
   131941 gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat
   132001 cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa
   132061 tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat
   132121 ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac
   132181 cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag
   132241 gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc
   132301 tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag
   132361 attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa
   132421 tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga
   132481 ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca
   132541 aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt
   132601 tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat
   132661 tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta
   132721 ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc
   132781 gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt
   132841 tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag
   132901 cagttatttt cactaatggg tccattacag attcagctac tttaggagat tacgaaacac
   132961 cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac
   133021 gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg
   133081 tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga
   133141 ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag
   133201 gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca accgcatttt acgtctcaga
   133261 aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg
   133321 tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac
   133381 tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca
   133441 gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg
   133501 ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag
   133561 aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag
   133621 aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt
   133681 acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag
   133741 gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta
   133801 tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa
   133861 gaggttatga aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc
   133921 ttatgttttt gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac
   133981 caagaggtta tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt
   134041 tcctcacgtt tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac
   134101 ctctttttat ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct
   134161 tgccaatcca acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa
   134221 atcggaaaca cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa
   134281 aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac
   134341 taataatgca gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg
   134401 gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac
   134461 agatgcaatc atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca
   134521 tcatggtgaa gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt
   134581 tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt
   134641 taatattagt tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga
   134701 atcaaatcca gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca
   134761 ttggggtggt caagcggaat atctaagagt tccttatgca gactctactt catttgttgt
   134821 accagataat gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc
   134881 ttattggagc gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc
   134941 tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat
   135001 cgcagtagat ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac
   135061 ttatttctta gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg
   135121 cgcagatgtc attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc
   135181 gaaaaattta gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc
   135241 tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta
   135301 cccgctgcaa gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat
   135361 ccatctaatg ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat
   135421 cattactcat tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa
   135481 agaagataaa aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat
   135541 tttctttatg cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga
   135601 tatcgaaata cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc
   135661 actggctgtc gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac
   135721 taaaatcccc gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat
   135781 tcgtcctatt ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa
   135841 gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac
   135901 cctatcgtat cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc
   135961 aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt
   136021 tcgtacccaa gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc
   136081 tgcttacgct tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg
   136141 ttatggaagt cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag
   136201 accacatacc attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca
   136261 tacacaacag caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat
   136321 tctgggtgtt ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa
   136381 aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt
   136441 gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat
   136501 ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca
   136561 aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca
   136621 atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta
   136681 aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg
   136741 gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat
   136801 tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg tttttacgtc
   136861 ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct aagttgacaa
   136921 agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgtccagct gcgattgcat
   136981 taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg accatattcg
   137041 ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt aaggcagatg
   137101 gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa gaacctacga
   137161 tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca gagatagtga
   137221 tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca
   137281 ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt gaaataatcc
   137341 caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg cctaaagcga
   137401 agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag ccgttagcta
   137461 aatgataagc aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag
   137521 cagcagcccc gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga
   137581 acccgggaat cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca
   137641 tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg
   137701 ctacaggtat aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt
   137761 ttctttactg tagattacgc gttcaatcga cgatacagat taatgatctc ttttgctaaa
   137821 tctgtaaaag ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact
   137881 tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg
   137941 actaaagatt cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc
   138001 gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc
   138061 atgatggctt ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta
   138121 tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc
   138181 agccatattg attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt
   138241 ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg
   138301 attatccgca tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc
   138361 tgccttttgc atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat
   138421 gattgttttc ttttccattt taaacacgtt cctttctata cttacacttg tatataagca
   138481 agtagcgtgc caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat
   138541 accaagttgc atacacacta aaaaagaaag cgcacactaa acacacaaat taatttttat
   138601 tacctttttt cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt cacaactttt
   138661 ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa
   138721 cataagatgt tgtttttact ttgctattct tattacttga tgcaggacag ctttttcaca
   138781 acctctcaat aaacggtgtt caatcagctg accctgggta ttagctcaaa acgctggatt
   138841 cacaacctct tttatttcaa ataaccagta aatacaatga acatctacaa aaaacttcta
   138901 tttatttata gatttcgtca ggcaaatcca attctctctt atcaatcaaa gtctgatacc
   138961 acgaagcaga ttttttcaat gttctgcttc gttcatcgtt caattcgatt cgaactaatc
   139021 catatcgatt cttaaatgca ttcattggtg acacacagtc agtgaatgcc cataacatat
   139081 agccttcgca atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga
   139141 taaattcgat acggtaatca tcttgcacga ttcctttctc atccatatac tgttcttcat
   139201 attctcttcc cataccgttt tctgttatca gccaagggat attatgataa ttttctttta
   139261 gatacatggc tatatcatac atgattttcg gatagatttc ccatcccctg gaattattca
   139321 ttttcttccc aggtaaatca aagacttcat aatatcgttc aggatggaaa ggtgtctctt
   139381 cattccattg ataacgaggg gcgcgaacgc gtttaggata atattgattg attccaagga
   139441 aatctactgt atgatgctga atcgtttcta aatcctctgt tgcaggatcg aaataaatcg
   139501 attgtttttg acacaactcc aggaactctt ctgtgtagta tccttttacc atcggatcaa
   139561 aaaatactcg attataaaac aaatcataca tataagcagc tttttggtct tctggagaat
   139621 tggatcgagc atagaccatt tcaggattta aaatacagcc aatccttcca ccataatttc
   139681 cgctgcggta aatctctact gctttagccg ttgctaaaac tttatgataa ttccacatca
   139741 tccacttttt tgtgttttgt tcatgcggcc agcgaacagc atccaaatag caacgcgttt
   139801 ggggaacgat tggttcgttg aatgtaaacc agttttttac cctatcaccg taacgatcaa
   139861 aggcaatttg agcatactta gcataaaaat caactacttt tcttgaagac cagccatcat
   139921 atttttccaa aagaaaagct gggacttcat aatgttccaa acaaatcatc ggttctacgt
   139981 ctgcttcgat caaagagtcg atcatttcac ttatatgctg agcatattct tcatctacta
   140041 tacagttctc ataatcagta aaaaaacgag cccaattgat agaagtccga taatgtgtca
   140101 gatggatgga tttcatcaga tccacatcct gctggtattg ttccataaaa ttagttgcta
   140161 cggctgggcc atacccatta tgccaaacaa aatgttcatt tttataccag ctatctataa
   140221 acgaatcctg tccttctttt tttcctttcc aaccttccgt ttgccaagca gaagatgcag
   140281 caccaagaat aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt tttttagatc
   140341 ccatcatttt ccacacgatt tgctactttt acaaatggca gatagatcaa aatagaaaca
   140401 ataatacaaa caagttgcgt aataactgca ccgatatttc ctgctgttgc cagataagca
   140461 ttgacgatcg gcggcattgg ccaagggacc attacaactg ctttcgccgc aaatcctaaa
   140521 gcggttgcaa aataaccaat cgttccagtg ataagaggcg ttaagataaa cggaatagct
   140581 aaaattggat tcagcatgat cggcatacca aaaataactg gttcattgat gttgaagatc
   140641 ccaggagcaa gggataactt ggtgatttgt ttcatatctt cccgttttcc aactaacaat
   140701 actgcaatca gtaaaccgat cgtgactcca gacccgccca tactcatata catatcccaa
   140761 aatggcatag taataatgtt cggtacttct tttccagctt caaaagcttc cgtatttaca
   140821 gcaattgccg ctaaaagcaa cggttcccgt atcggcttca ccatttgatt tccatggata
   140881 ccaatcaccc aaaagacttg cgcaataaac atcaatagca aaatacccgg taacccttgt
   140941 acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag gtacattcct
   141001 gtaagttctt tgatgataaa tcccgcagcc gcgatacccg atacagtcag aatcgtcggg
   141061 aataaagcag aaaaactagc agaaacattc gttggcacgg tatcaggcat tctcagctgt
   141121 agtttcttct gtttcctcaa ccagcaataa agttcaatag aaaggatgga gaccaacatt
   141181 cccaaaaata accctttcgt atctgtatat tgtttagcca atacgttttc tactataaca
   141241 gtctgatttt cattcactaa ttcataaaca gttggattaa cagataaata agacatcact
   141301 gcgatgatac cagggaatac accatccact ttattcagct tggctaattc gatccctatc
   141361 agaaaaacag catagatggt caagaaactt agtgtgacat agctgatggc agatgcgata
   141421 ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt ttccgagtca
   141481 aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca
   141541 gcaaatgcgt tttttatcac catgatatac cttaaattat tgattctcgt tgccacgttc
   141601 cctaaaacat tggctaattt atccataaaa ctcattccag atcccctcat tcctgcttct
   141661 tctccttgac gtcatctctt tttgtctatt agtcagcgct tacatttcga tgaacagaaa
   141721 ctatttactc ctctttttta ccaaatataa aacaaaaatt catctgatgt atttatcata
   141781 aaatacatca gatgaactgt caattagttt ctttttctcc tattattttc tgtaattctc
   141841 tccgatttat tgccatatgg acagtcattg catcaaacgc agcaagtgca ttccgccttt
   141901 cgatggcctg aacgatctct gtatgaatat cgatcgtctc gattttcaga tgctggatat
   141961 cataattttt attaaacaaa gcaattgatt cattaatgat tgggataata tgcagcaagg
   142021 ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt
   142081 cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc atttggacga
   142141 tttgattctt cgttgcatat tccgctgcca aacctgccat cttaggctcc aataaatatc
   142201 tcagttcaaa taagtctcgt atcatccgat cctgatcttc aatcaaagaa aaaccgagtg
   142261 ggtcattggc aatccctcgc ttatcactga caaatgtccc cgctccttgc cgaacttcaa
   142321 ggatattcct ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc cccacctcta
   142381 aataatcgga caattcatat tcattcggca gtttatctcc tacttgcaac tgcatttcct
   142441 ttaaatatct aaggatcttg tcagtagtta cttctactag ggttttattt tcttccaaac
   142501 cacatacctc cttttcatct gatgtatttt ttcgtaatta ttataacacg tttgaatcat
   142561 ctgatgattt tttgtataca aaattgatta atccgttcat agacatacaa acgaagaaga
   142621 tatttaccat aaaaaagact aggataaaat tcactcctag ccttcgatca tttttatttt
   142681 gaacgacaaa aaatatgatg ttcaataaca gacgatcttc attcgattct tgatgtcatc
   142741 ttttgttttt tttaatatat ggataaaaag tttgattaca tcgtttctat tctagaattt
   142801 ggtcgattct caattcttct tctggtgaga aatctaaatt atccatcatt cgaacacttt
   142861 cttgtaactg acttaaccga cttgctccga ccagtacact tgtaacagat ttttgtcgca
   142921 aattccatgc aagggccatt tgcgctaatg attgtcctcg tgatacggcg attgtttgca
   142981 gtgctttgat tttttccaga gttgatccaa cttgttcttc tgaaaggaat ggtatttcct
   143041 tacgatgtgc tctagaatct tcaggaatac catgtagata acgatttgtc agcaaccctt
   143101 gagcaagcgg actgaacgtg attgccccta gcccttcttc ttctaatact ggagatagat
   143161 cgtcttctag tagtgcacga gaaaacatat tgtagcgcat ctgatgaata atgaacggtg
   143221 cttcctttct ttttaaaatt tcagtcattt tttttgtatc ttctccatta tagttggata
   143281 taccaatata taatgctttt ccttgtctta caagttgcat caaagcttct gctgtttctt
   143341 ccaaaggtgt atcagggtct gggcgatgat gatagaagat gtcaacataa tctagcccca
   143401 ttctttggag actttggtca caacttgaaa tgatggattt acgagagccc cattccccat
   143461 atggcccttc ccacatatga taacctgctt tgctagaaat aatcagttca tcacggtagg
   143521 cttgaaaatc ttctctaaaa atacgcccaa agtttttctc tgcagctcca gcagggcctc
   143581 cataattatt cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct cttaaaatct
   143641 ctctttgatt atggatagga tcataatcac caaaattttg ccataatccg agagataaaa
   143701 tcggtaattt caaacctgaa tgaccggatt tacgatattc cattttttca taacgtgcac
   143761 tatctgcaat atacaatttt tctcctcctc atcaaatctt tttaaaacgc tttctttttt
   143821 ctatttttag tttactacat tttaaagaaa aggagttaat caaaatgatt ttttcgtata
   143881 tttttttgcc atttgttttt tactcgttga tagatcagaa attgattacg tgcttgctgg
   143941 atttttttat aacgtttcgg ttgttttttg taaaattgct gatgatagtt ttctgctggc
   144001 caaaacgtac cggctggttc gatttttgtg acgattggtt ggtggaaagt tcctgaactg
   144061 tctagtttct gttttgattg ttccgctaat tctttttgtc gttcgacttg ataaaaaatc
   144121 actggacgat attgcttccc tctatcttga aattgtccaa atgtatctgt gggatcagta
   144181 atttgccaat agattgtcag caactcgcta taacttattt ctcttgtgtc ataaatgatc
   144241 tccacagctt ccacatgacc tgtgtaacca ccacttactt gatcataatt tggtttttca
   144301 acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc cttttttcga ttcaaacggc
   144361 tcgaccatac accagaaaca tccccctgca aatactgcac gttcttgata gtcagcatct
   144421 tgcatggcat gcttggaaaa gtcatatttt tgctccttat caaatccttc cgtaatcaat
   144481 aaataaaagt ccattacatc aggcgtcaga ttcattcgga tagctaatgg acgtagtgtt
   144541 gcttctagtt ttgataactg ttctttaacg gatatatttt ctcttatgtc attctttgtc
   144601 tgaatcaata cttttcgttc ccaatctcgt gtattcggat tcaaaatcag attataaagt
   144661 gtttgaatga atatttgttc ctcatccata gattctcctc ctttccgaag aagacaagct
   144721 agtgacttta gctgtaattt atcataaagt gaggaaataa gcgattactt catcccccta
   144781 cagatttttc gtataaaaaa gagagaatcc ttatcgacag attctccttt ttaatcaatt
   144841 tattttataa atgaaaagtt aatttacctt ttatggaaaa ctattttaaa aaataaacat
   144901 aaatatgcag ttaatgtaac aagataagaa ataatagtcg ttactgtata acttgtgctt
   144961 actttaatat cagcaactcc tttgactccg cttatttgta ccaatccatt agcattcacc
   145021 tgctccttta caggttgatg atttatttct acattcgttc ctaaataagc atatacagga
   145081 agtgtgatta cttggctctt ctcattagtt actctatata tgatagaact accagttgtt
   145141 ttataaggaa ggtttaccca ttgactgcca atctttgctt cgtgttttat tatatgctcc
   145201 ttattctcca atgaagcgat aggagaataa tcatataaaa aataatccat tcctttagtg
   145261 gtatacgcct ctactgtttc tttgtttaca acaatatgtc ccggctgaga aattattttt
   145321 tgattgaaat tttttttact agaaacagtt aaagtgatta ataacaaaac agtcataaat
   145381 aaataaaata gttctcttgt cttattgtat cttctatttt tgaaaaatat catcgttaat
   145441 atcagagaag agaatattat ttgcaagctt aagatccgcc aaggaaattg taacaatttg
   145501 gctggcgtat tttgcagaag ataccatggc accaaactag ttgtagctag ccaagtcaaa
   145561 cacgtaccat aataaatctt tttttctatg tttgttaatt ttttaaataa aatcagggga
   145621 atacataaag ataacagacc aactagtccc attgagtaag tagttaagtc gttgttcata
   145681 gcttcagtca gactatcgaa cacattagat gcatgagcat ataaatttgt cacgctaggt
   145741 ttgttgattt tttggtagag catttgttca aacatcggat accaaaaagc actcgtcaga
   145801 accaatgtca ttcctgccgc actaaataat tttttaaata tagcaataaa atctgcattc
   145861 ttgaaacctt tagaaaccaa acgaataaaa ataaaaatga taattaatgc tgtactcatt
   145921 aatgttgata gtacatggct gtacactagc aaactcatac tcaatgctag ctttacagac
   145981 ggatactttt cttcttttcc agagatgatt tgataagcat aaaataatac agggataaca
   146041 agtgttattg cgatagcttc ggcaattgcg gaacgatagt agatgtctac tgtccgataa
   146101 gcagagaaaa tatagagaca actaaataaa aatgccgctt ttttttgttt taaaaaccgc
   146161 tcgccataat gataagctac gagtgttgta cacaagttca ataaccaaac gtaaataata
   146221 tagcttacaa tcaaattttt tgtcatccaa tataacatta cagctggaaa aaaagtcaga
   146281 aagggataaa aataatttac accatatcca gtataattga atgtctcata attaatcgga
   146341 ctggtaaaaa tagtcgataa ccccttgatt cgagataagt gaaaagaaag atctccagtg
   146401 gtataaatac tctgtgtatt tttgaaaaag atgaaagaat atatactact aactataatt
   146461 aatccactca atattactac ttttttattt attttcattc ttcttgttta tctccttttt
   146521 agtcacatat cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac ttgaatacac
   146581 cactaaacaa taagtcagaa tactgatcca taaagccatg agagcagttg gagtatattt
   146641 tgttgtaatt tctatcttat tttctccagc atttacatcc acattcacta agccatgttt
   146701 tttataagag tcatttacga ctttaccgtt tacttttacc tgtgtaccta aataagcata
   146761 tactggtact gtcacttttt gctttttgtt gcttgccact gtgtaagtaa tgttattatc
   146821 tgcagcttta tacttccctt tttcccacga ttctcctact tttacttcat gtttttctaa
   146881 gtgaggcctt tcttttatcg cttctgttgg ggcgtaatcg tataatcctc ccatatctga
   146941 tgttgtataa cgtgggagtg tttcttcatt gataacaata tgaccgtgtt ttgattggat
   147001 aacttttgcg taattttctt ttgctgctat agacgtgacg agtagaaaca ccacagtagc
   147061 gccaatgatt aaccatgaat attttttatt catagtcttt ttcataaaaa caagcgttat
   147121 gatcattgaa gaaaaaaaga tttggatacc taagatacgc caaggaaact ggagtaagct
   147181 tatcggtgtt ttttgaaaca aaaaccatgg aaataaatta gttacgcata cccatgtaga
   147241 aactacgcca acatagatga ttttttcttt tttctccatt tctttcagta tgaataaagg
   147301 aaaaatcaac gcaaccaatc ctaataaccc catagtgtat gttgtaatat cgttatttaa
   147361 cgcttgaatg atactatctg aaatagaaag tgcttctagc tgcaaatctg ttttgaaggg
   147421 actgtgtatt tcttgataat tcatctgctg caacatagga taccaaaaat agctagttaa
   147481 tgcacaagtc aaaaaaacag cctttactaa attcacgacc acaaatttta tatcgttttt
   147541 tatttgcttg cttaaaaaca aacgaactat aatcatacaa gcaatcaatg cactacacaa
   147601 tagtgtagtt aagacatgag tatacacaag tagacttata ctgacagata aagcgatgac
   147661 ttttttttct ctattgaata tcaatccata agcataatat aaaacagctg ggataatcgt
   147721 aagagcgatt gcttctgcaa ttgcggatcg ataatagata tcaactgttc gataagttgc
   147781 aaaagtatat aaacaactaa ataaaaaagc agcatctatc tttttaaaaa acttttctcc
   147841 ataataaaaa ctaatcagaa tcgtacaaat atttaataac caaatatata gaacataact
   147901 taatattaag ttatttgaaa ttccataaaa aacgactgca ggaattattg taagaaaagg
   147961 ataaaaatag ttcagtccat ctccataatt gttaaatgtt gtatagttga ttggcccttc
   148021 aaaaatagaa gataaccctt tgattcgtga aagatgaaaa gataaatcac ctgttgtata
   148081 aatttcttga gcatttctca taaaatttac tgaatataaa gtactaagta agataagaac
   148141 tactaaaaga aatgcttttt tacttatttt catagatttg ttgttctcct tttttaattc
   148201 tattttcagt atactagaat tgtctttttt taataagttt tgcgttaaat ttttcatagc
   148261 actagtatga tatactagaa aagaatcaat ttttattact tttaaatagg agaaaaaaat
   148321 gaaaaaaaac aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta
   148381 cgtcatccct ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt
   148441 tcatttatct cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa
   148501 cttcgatcat catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc
   148561 ttttttattt tataaaatga tgggaaatct tgttttaagt tataatatct actatttttt
   148621 tatcactttt ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg
   148681 ttatatttcg ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat
   148741 attccatcgt gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat
   148801 ggggtgttac gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat
   148861 gacgctagta gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg
   148921 aacgttgttt ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt
   148981 aaaagctacc gtcctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca
   149041 atctagagca caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag
   149101 tgatatgctg acacatattt taaataataa ctataacaac tatactattg gattattctt
   149161 atttctcgga ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt
   149221 tattttcttt cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact
   149281 ttttaaccat acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc
   149341 tttatttata gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa
   149401 ttcatggaaa ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag
   149461 caattctatc catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc
   149521 tatagctgaa aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga
   149581 tatgattaat aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt
   149641 tatgagtaac aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac
   149701 gacacctatt tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac
   149761 aaaattatct aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt
   149821 tattacttat cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt
   149881 gattttattt ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt
   149941 acaacattct cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc
   150001 taaaaagtct ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt
   150061 atactgtgaa acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat
   150121 ccaaaaacag accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa
   150181 atatttcatc gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca
   150241 tgataatccg caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt
   150301 ctccctttcg ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt
   150361 tcaatgcaga aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca
   150421 gcttgtccat atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag
   150481 gaatcacgtt gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat
   150541 ggaagcgctc aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat
   150601 tcataaagtc gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt
   150661 gtcctcgcgc atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat
   150721 ttgtcatctg atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa
   150781 gtctttgcca tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca
   150841 tattttagtg gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc
   150901 acaaaatata gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat
   150961 atatttcaga aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga
   151021 cggctcaagt tttactgttt gagatgcaag atactcaacc tggcaatggg cacataatta
   151081 aagaaaaaac tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata
   151141 gaaggaattc ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca
   151201 aggtataagt ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact
   151261 ggaaacaata aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag
   151321 acattccacg aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct
   151381 ataccataga acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca
   151441 aatgcagcat acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa
   151501 ataagaaacg cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc
   151561 atttgatcta attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc
   151621 acgcctaata ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa
   151681 taacaattac ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa
   151741 cctctattta cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact
   151801 aaatatctaa cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt
   151861 ccccacttct tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg
   151921 aatttaaggc agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga
   151981 tttgcatcgc gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca
   152041 gtgaaccgcc cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa
   152101 ggactttcaa aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca
   152161 aggaaatggt aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg
   152221 aataagactc ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg
   152281 cggcagaact tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca
   152341 atggataggg attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga
   152401 caaacagcat cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga
   152461 ttccagtcgt agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa
   152521 taatcacttg tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg
   152581 attctgaacg tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt
   152641 aattcgcatc aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata
   152701 aaacactttc caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa
   152761 cagtaaaaat tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat
   152821 ataagatcaa aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg
   152881 ccatcgtcaa gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg
   152941 ttcccaatat tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta
   153001 aaataccaac aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac
   153061 caccctttca caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa
   153121 ttttctaaaa attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt
   153181 ctacacgttg ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg
   153241 aaacgttttc ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata
   153301 aagaaggaga gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag
   153361 cgtatattga tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac
   153421 atctatacat ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg
   153481 ggttaccaat atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc
   153541 tttatattcc taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc
   153601 ttgaatattc gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat
   153661 gctgtttctt gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa
   153721 ttatcgaaaa caacaattaa atatctacta ttatttttaa caattggatt agttggctgc
   153781 tctactctat ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt
   153841 ttttatttat ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc
   153901 tataagcttc ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg
   153961 acacaaattt cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca
   154021 tattctctct tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct
   154081 gataaattag gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa
   154141 gttatatgta atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt
   154201 ccaaactcag ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta
   154261 gcagtgtccc tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt
   154321 ttcatcgaat acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga
   154381 attcattggc tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt
   154441 accttcgtgc agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt
   154501 gccttaaaaa tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg
   154561 ggatttttct tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc
   154621 ttgatcatta acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag
   154681 cctgtctttc cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt
   154741 agcccagtct tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt
   154801 attgtacaac gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt
   154861 atcagtatcg ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat
   154921 tcgccttctt tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa
   154981 ggcgccgtaa aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc
   155041 atggaagaac ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac
   155101 acaaaggcag atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt
   155161 ctcaatttaa gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca
   155221 gaccctattc cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc
   155281 gtcggttatg gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc
   155341 agtaacctgt ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata
   155401 agtccaaata ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct
   155461 ctttatcgca gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac
   155521 agtccggata aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat
   155581 tcagcataca aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc
   155641 caattatcca ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac
   155701 actgctgaag gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa
   155761 ggccttcact ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat
   155821 aaaccgatta cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat
   155881 tcgaaaaaat atggattaga acagcaccag acagattatt tcatttataa caacaagtat
   155941 agtcgtgaac aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct
   156001 atggctcttg agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta
   156061 tctaatcatc tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat
   156121 ggaaaacagg tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag
   156181 aaacaaaaag aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac
   156241 cagtacagtg caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg
   156301 ggttgtgata aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa
   156361 acgatttgat taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa
   156421 ttatcgggat tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac
   156481 ccgaatagat agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct
   156541 gtgatttgat agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact
   156601 ccaccaatag ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat
   156661 aagtccacac tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat
   156721 ttggtcataa atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact
   156781 tgttcgctgc tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt
   156841 tgagttgttt cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta
   156901 cttgctgcag ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc
   156961 gctgtttctt ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa
   157021 aaagctagac caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac
   157081 tcccacttct ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc
   157141 atcaatcttt caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat
   157201 cagcgggtgc ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat
   157261 ttgtatttga agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg
   157321 gacagttttt ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag
   157381 ttcaacaagc aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg
   157441 tgtgataaag acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc
   157501 tggtaaatat ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa
   157561 agtagacgga acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct
   157621 cgcttcttga tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa
   157681 aacatacaat tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg
   157741 ccccaccatg gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg
   157801 aacaaatttt tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga
   157861 cttcataacc taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat
   157921 caaagtttgc tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct
   157981 gttgagaaac cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt
   158041 gcataataat ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga
   158101 ttacagcttc cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt
   158161 cgtgtccaaa atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt
   158221 gaacgaacat cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata
   158281 agacgacacc aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca
   158341 agcggtaggc atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt
   158401 cgatagcaac atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg
   158461 gagaatcatt gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt
   158521 tcacaaattg aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca
   158581 tatcagctac ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg
   158641 ctcctgcttg tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca
   158701 cacctatgat ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt
   158761 gttgtaattg atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa
   158821 tcaatttggt gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc
   158881 ccttgattgt ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag
   158941 cataatggat cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta
   159001 aagctaagct gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc
   159061 ctttggtcaa tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca
   159121 ttgcttcacc gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat
   159181 tcgaaacagg cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg
   159241 ctaaacgaaa atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag
   159301 tataatactg agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt
   159361 cttctactaa ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa
   159421 tcgtcacgta gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga
   159481 cgacttgtcg tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga
   159541 ctcgtccgtc cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca
   159601 cgtcatcgat ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg
   159661 gggccatctc ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca
   159721 gatagctacc aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca
   159781 taaaggcacc aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca
   159841 ctgcctgata cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag
   159901 caatgttata gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca
   159961 cagctgataa ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct
   160021 ttctccctct tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa
   160081 aattgaccac tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa
   160141 aaaatagtta gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg
   160201 aaattttcat tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa
   160261 gatttttatc ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg
   160321 atacgtacag ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa
   160381 ttctgttagc aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa
   160441 aatgatctct ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc
   160501 tgtctcgtaa gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt
   160561 aacatatatt tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag
   160621 caaaacacct gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa
   160681 ctccatatac aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct
   160741 ctcaaagttc attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa
   160801 gggtttaaaa gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta
   160861 cattataata gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa
   160921 gcgacagctt tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga
   160981 cgcgtcaaaa aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa
   161041 aagattcaag caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact
   161101 ttttatacac attataagga tatctatgat ctatatgatc aattagaaag caactttttt
   161161 cgggatctaa atgaaattct ctcttatgat ccagcccata gctatgagga actatacact
   161221 cgcttgattg actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat
   161281 ggtgatattt cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata
   161341 tggctatatg aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac
   161401 cacgtccaag gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc
   161461 aaaaaagaat tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct
   161521 tgaataaaaa catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga
   161581 atcttattta gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac
   161641 ttattgaaaa gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac
   161701 caatcaaatt cacctcatca ttaataaatt acttttaggc taatttatta caacttatag
   161761 gttgaagctg taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc
   161821 ttctcgttta cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc
   161881 atgctcgctt catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat
   161941 atccttcgta atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg
   162001 gcatatattg cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa
   162061 agacatgaat caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat
   162121 cttcattgct atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc
   162181 aataaagatt tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct
   162241 ttgttttgaa acatcttctt cccctct
//
LOCUS       DARDHU010000002        94264 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000002 DARDHU010000000
VERSION     DARDHU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.002"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SDD03_000144"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SDD03_000144"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG4302625.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SDD03_000145"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SDD03_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG4302626.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SDD03_000146"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SDD03_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG4302627.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SDD03_000147"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SDD03_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG4302628.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SDD03_000148"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SDD03_000148"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG4302629.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SDD03_000149"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SDD03_000149"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG4302630.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SDD03_000150"
     CDS             complement(8053..8523)
                     /locus_tag="SDD03_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG4302631.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SDD03_000151"
     CDS             complement(8569..8994)
                     /locus_tag="SDD03_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG4302632.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SDD03_000152"
     CDS             complement(9061..9330)
                     /locus_tag="SDD03_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG4302633.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="SDD03_000153"
     CDS             complement(9341..10936)
                     /locus_tag="SDD03_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4302634.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SDD03_000154"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SDD03_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG4302635.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="SDD03_000155"
     CDS             complement(14489..15049)
                     /locus_tag="SDD03_000155"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG4302636.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="SDD03_000156"
     CDS             15402..16376
                     /locus_tag="SDD03_000156"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG4302637.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="SDD03_000157"
     CDS             16546..17946
                     /locus_tag="SDD03_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG4302638.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="SDD03_000158"
     CDS             complement(18024..18746)
                     /locus_tag="SDD03_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4302639.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="SDD03_000159"
     CDS             complement(18825..19331)
                     /locus_tag="SDD03_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG4302640.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="SDD03_000160"
     CDS             19454..21253
                     /locus_tag="SDD03_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG4302641.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="SDD03_000161"
     CDS             complement(21321..22895)
                     /locus_tag="SDD03_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG4302642.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SDD03_000162"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SDD03_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG4302643.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SDD03_000163"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SDD03_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG4302644.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="SDD03_000164"
     CDS             complement(24672..25310)
                     /locus_tag="SDD03_000164"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG4302645.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="SDD03_000165"
     CDS             complement(25311..25961)
                     /locus_tag="SDD03_000165"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG4302646.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SDD03_000166"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SDD03_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG4302647.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SDD03_000167"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SDD03_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG4302648.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="SDD03_000168"
     CDS             complement(29104..29844)
                     /locus_tag="SDD03_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG4302649.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SDD03_000169"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SDD03_000169"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG4302650.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="SDD03_000170"
     CDS             complement(31215..32153)
                     /locus_tag="SDD03_000170"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG4302651.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="SDD03_000171"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="SDD03_000171"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG4302652.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SDD03_000172"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SDD03_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG4302653.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="SDD03_000173"
     CDS             complement(35313..35618)
                     /locus_tag="SDD03_000173"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4302654.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SDD03_000174"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SDD03_000174"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG4302655.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="SDD03_000175"
     CDS             complement(36724..37008)
                     /locus_tag="SDD03_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302656.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="SDD03_000176"
     CDS             complement(37026..37916)
                     /locus_tag="SDD03_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG4302657.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="SDD03_000177"
     CDS             complement(37936..38154)
                     /locus_tag="SDD03_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG4302658.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="SDD03_000178"
     CDS             complement(38124..39824)
                     /locus_tag="SDD03_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG4302659.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="SDD03_000179"
     CDS             40091..40864
                     /locus_tag="SDD03_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302660.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SDD03_000180"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SDD03_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG4302661.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="SDD03_000181"
     CDS             complement(41688..42659)
                     /locus_tag="SDD03_000181"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG4302662.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SDD03_000182"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SDD03_000182"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG4302663.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SDD03_000183"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SDD03_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG4302664.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="SDD03_000184"
     CDS             complement(45614..46438)
                     /locus_tag="SDD03_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4302665.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="SDD03_000185"
     CDS             complement(46411..47091)
                     /locus_tag="SDD03_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4302666.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="SDD03_000186"
     CDS             complement(47114..48052)
                     /locus_tag="SDD03_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG4302667.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="SDD03_000187"
     CDS             complement(48297..49160)
                     /locus_tag="SDD03_000187"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG4302668.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="SDD03_000188"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="SDD03_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG4302669.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="SDD03_000189"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="SDD03_000189"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG4302670.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="SDD03_000190"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="SDD03_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG4302671.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="SDD03_000191"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="SDD03_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG4302672.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="SDD03_000192"
     CDS             complement(56026..57267)
                     /locus_tag="SDD03_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG4302673.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="SDD03_000193"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="SDD03_000193"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG4302674.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="SDD03_000194"
     CDS             complement(58294..59217)
                     /locus_tag="SDD03_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302675.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="SDD03_000195"
     CDS             complement(59956..61242)
                     /locus_tag="SDD03_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG4302676.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="SDD03_000196"
     CDS             complement(61235..62503)
                     /locus_tag="SDD03_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG4302677.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="SDD03_000197"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="SDD03_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG4302678.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="SDD03_000198"
     CDS             complement(63276..63722)
                     /locus_tag="SDD03_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4302679.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="SDD03_000199"
     CDS             complement(63815..64060)
                     /locus_tag="SDD03_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG4302680.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64175..64753)
                     /locus_tag="SDD03_000200"
     CDS             complement(64175..64753)
                     /locus_tag="SDD03_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4302681.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64830..65360)
                     /locus_tag="SDD03_000201"
     CDS             complement(64830..65360)
                     /locus_tag="SDD03_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG4302682.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="SDD03_000202"
     CDS             complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="SDD03_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG4302683.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66217..66612)
                     /locus_tag="SDD03_000203"
     CDS             complement(66217..66612)
                     /locus_tag="SDD03_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG4302684.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66609..68021)
                     /locus_tag="SDD03_000204"
     CDS             complement(66609..68021)
                     /locus_tag="SDD03_000204"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG4302685.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68018..68554)
                     /gene="cysE"
                     /locus_tag="SDD03_000205"
     CDS             complement(68018..68554)
                     /gene="cysE"
                     /locus_tag="SDD03_000205"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG4302686.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68640..68879)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(68876..70336)
                     /locus_tag="SDD03_000206"
     CDS             complement(68876..70336)
                     /locus_tag="SDD03_000206"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG4302687.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70506..71663)
                     /locus_tag="SDD03_000207"
     CDS             complement(70506..71663)
                     /locus_tag="SDD03_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG4302688.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71697..73067)
                     /gene="radA"
                     /locus_tag="SDD03_000208"
     CDS             complement(71697..73067)
                     /gene="radA"
                     /locus_tag="SDD03_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG4302689.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73130..73657)
                     /locus_tag="SDD03_000209"
     CDS             complement(73130..73657)
                     /locus_tag="SDD03_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG4302690.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73887..74693
                     /gene="proB"
                     /locus_tag="SDD03_000210"
     CDS             73887..74693
                     /gene="proB"
                     /locus_tag="SDD03_000210"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG4302691.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74690..75937
                     /locus_tag="SDD03_000211"
     CDS             74690..75937
                     /locus_tag="SDD03_000211"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG4302692.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76013..76357
                     /locus_tag="SDD03_000212"
     CDS             76013..76357
                     /locus_tag="SDD03_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302693.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76474..76800)
                     /locus_tag="SDD03_000213"
     CDS             complement(76474..76800)
                     /locus_tag="SDD03_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302694.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77415..78617)
                     /locus_tag="SDD03_000214"
     CDS             complement(77415..78617)
                     /locus_tag="SDD03_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4302695.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78734..79249
                     /locus_tag="SDD03_000215"
     CDS             78734..79249
                     /locus_tag="SDD03_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4302696.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79448..79894)
                     /locus_tag="SDD03_000216"
     CDS             complement(79448..79894)
                     /locus_tag="SDD03_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG4302697.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79941..81419)
                     /gene="msr(C)"
                     /locus_tag="SDD03_000217"
     CDS             complement(79941..81419)
                     /gene="msr(C)"
                     /locus_tag="SDD03_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG4302698.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81773..82402)
                     /locus_tag="SDD03_000218"
     CDS             complement(81773..82402)
                     /locus_tag="SDD03_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4302699.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82386..82850)
                     /locus_tag="SDD03_000219"
     CDS             complement(82386..82850)
                     /locus_tag="SDD03_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4302700.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83337..84605)
                     /locus_tag="SDD03_000220"
     CDS             complement(83337..84605)
                     /locus_tag="SDD03_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG4302701.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84836..85573)
                     /locus_tag="SDD03_000221"
     CDS             complement(84836..85573)
                     /locus_tag="SDD03_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4302702.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85640..86209)
                     /locus_tag="SDD03_000222"
     CDS             complement(85640..86209)
                     /locus_tag="SDD03_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG4302703.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86337..87209
                     /locus_tag="SDD03_000223"
     CDS             86337..87209
                     /locus_tag="SDD03_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4302704.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87551..88423
                     /locus_tag="SDD03_000224"
     CDS             87551..88423
                     /locus_tag="SDD03_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4302705.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88420..90378
                     /locus_tag="SDD03_000225"
     CDS             88420..90378
                     /locus_tag="SDD03_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4302706.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90710..91561)
                     /locus_tag="SDD03_000226"
     CDS             complement(90710..91561)
                     /locus_tag="SDD03_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4302707.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91684..92151
                     /locus_tag="SDD03_000227"
     CDS             91684..92151
                     /locus_tag="SDD03_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG4302708.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92414..92896)
                     /locus_tag="SDD03_000228"
     CDS             complement(92414..92896)
                     /locus_tag="SDD03_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG4302709.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92996..93859)
                     /locus_tag="SDD03_000229"
     CDS             complement(92996..93859)
                     /locus_tag="SDD03_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG4302710.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        26337 a        20305 c        16934 g        30688 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaga aatgcaataa
    64141 aaataaataa gaagctcatt aagatttttc taaattattc ctcattataa aatttcaagt
    64201 gtttttttat tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg
    64261 ctaaacattc agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag
    64321 cttctatttt tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct
    64381 gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta
    64441 ttttttggtc gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc
    64501 tcaacagaga atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac
    64561 ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc
    64621 aatcctcaga aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa
    64681 aatatttttg atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata
    64741 actcctcaga cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa
    64801 tgattcatcg aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat
    64861 ttttgggaca atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata
    64921 aaatgcatat ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat
    64981 aattcataag cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa
    65041 tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg
    65101 atataactgt cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta
    65161 taattttgtt ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct
    65221 tcatatttcg cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg
    65281 tcctgctttt taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca
    65341 atcaacagct gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata
    65401 agcaaaccag cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc
    65461 agcaattcat ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg
    65521 actaaagcaa ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg
    65581 ccaaataccc aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg
    65641 cgtgcaacag ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt
    65701 ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt
    65761 aaaatcgacc caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct
    65821 tcttttttct cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta
    65881 atcgcaagaa cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta
    65941 ggaacccatt tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt
    66001 tctcctcgtg aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct
    66061 actgcggcat ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa
    66121 actgactgat ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca
    66181 aatccacgtt tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt
    66241 ctgaatacac cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata
    66301 gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc
    66361 gctcgtatgg cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa
    66421 aagatgttct tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt
    66481 cgttgccgca cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg
    66541 aacatagatt tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt
    66601 gtaatctctc atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc
    66661 cctttttctt tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt
    66721 gcttgatttc gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta
    66781 aataaaatac cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca
    66841 gcggcagaaa ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc
    66901 actgtgatcc catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct
    66961 aaattttcta acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct
    67021 tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct
    67081 ttcatcgtcg tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa
    67141 cgcaagactt gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga
    67201 gatttactca ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa
    67261 ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga
    67321 aattctaaat cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc
    67381 ataacggaac attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa
    67441 atctcgccag gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc
    67501 tgttcttccc ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt
    67561 aatttcccat aattttcaaa tttacgggta cggtaataca catcacctga agattcataa
    67621 gcatagcctt tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg
    67681 actcttggat gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct
    67741 cggatgaaac gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg
    67801 attttatcgt ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca
    67861 aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa
    67921 ttatacacag tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat
    67981 tcttcttttt ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt
    68041 ctcctttgat tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat
    68101 ctttcaacac aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta
    68161 atatctgcgc attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg
    68221 tttcttttcc tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt
    68281 ctgcggtttc gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg
    68341 ttgctcctgg atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg
    68401 ccaataaaaa gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat
    68461 gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct
    68521 ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt
    68581 ttatgtagga aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat
    68641 caaaagacgc tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc
    68701 taacgcctgt tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc
    68761 tgtgtttcgc caagaccact cccagaggca tttcggcaac cattcattga taccttgcac
    68821 cacccggtat ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt
    68881 tcagtacttg atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag
    68941 gcaattctgg accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt
    69001 ttccttttac gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat
    69061 ctactacttc catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt
    69121 caccagcaag tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg
    69181 tagacagatc aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct
    69241 tcttcaccca ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta
    69301 gataaggcag acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt
    69361 ttacccattg cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat
    69421 cgaacatttt gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc
    69481 ccaataatgc aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt
    69541 cgataaattg caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga
    69601 tgatcaatgt catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca
    69661 gttgttttgg tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca
    69721 aatgatcatc gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga
    69781 caaagtcgcc accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa
    69841 atgcgtattc tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt
    69901 ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt
    69961 ctcctcttgc gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt
    70021 tgtatgctcg attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt
    70081 cagattgacg gtaagggccg tattctccag gattttcagg agattcatcc caattcattc
    70141 ccaaccaagc aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt
    70201 cagtatcctc aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga
    70261 ataatgcagt tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta
    70321 cgcgtacttt cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa
    70381 ttccgtcagt tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac
    70441 cttttctaag gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt
    70501 cttttttatt ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt
    70561 cctgcatgtg caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt
    70621 acaacgactt cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg
    70681 ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca
    70741 ttcatcgttt caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta
    70801 agaacaggta cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg
    70861 acaccatcta aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca
    70921 aagtctccat cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata
    70981 tctaatccgc gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt
    71041 agttcgtaaa gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta
    71101 atatcataaa tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa
    71161 ttttctcctg attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg
    71221 atttttttgc ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt
    71281 ctcaatccca gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat
    71341 ggaatttcca tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc
    71401 gcaccgatcg caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact
    71461 tgtttgataa tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt
    71521 gcgcctaaca ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt
    71581 gcctgccaag ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc
    71641 aaagtaatga cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag
    71701 cggaacacac gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt
    71761 ggcgcttccc agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt
    71821 tggacttctt tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca
    71881 atttcaccga tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata
    71941 ctgacagcga tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt
    72001 aagtaagcat cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc
    72061 agagataccc gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta
    72121 ggtgtgacta atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg
    72181 atagcagagc cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg
    72241 acttcttcca atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat
    72301 cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac
    72361 accgtatcca ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca
    72421 tgaccgacga taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg
    72481 gtttctctta cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt
    72541 tggatggaat caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg
    72601 ttcatatctg tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg
    72661 cgtaatttta tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg
    72721 gctgctaatt gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca
    72781 ccaatcaaga ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct
    72841 aattttgttt tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt
    72901 gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct
    72961 tcgaccatcg tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca
    73021 tacccgcaat tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct
    73081 tttatcctaa tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg
    73141 aagaaccaaa tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg
    73201 gcaaaaagat cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa
    73261 agttggtgaa ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt
    73321 caatcacacc tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt
    73381 tagcaagctg caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg
    73441 ttggtttcaa ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt
    73501 ccaacccctt tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt
    73561 caaaatcata gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt
    73621 tatatttaga aataacttca aatcctcttt ttctcaccgg ttttcctccc taaataaact
    73681 actcctattc taacatatcc gaggctgtaa aagactttct ttacaaattc ttcaagaaat
    73741 tctgctatct taaagtaaac cattaattcg ttctttttcc aaacgactgc aagttgtaca
    73801 aaaaaactat tttttacatg actttactcg tcaaaacaag tagaatagaa cgaaattaat
    73861 gagtggatat ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta
    73921 ttgtcaaagt aggaacaagt acattgattt atcccaatgg aaatatcaat cttgcggcaa
    73981 tcgatcagtt agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag
    74041 tctcatcagg agctgtaggc gttggtatgc ataaattaca tttgtcagaa cgtccgactt
    74101 tgattcctga acagcaagct gcggcggctg ttggtcaagc cgaactgatg aatatctata
    74161 atcaaagatt cagtgtatat aatcaacaga cggcacagat cctcgtgaca agagacgtgg
    74221 tagattatcc tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt ttgattatgg
    74281 acacgatccc tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta gatcatctga
    74341 caaaatttgg cgataatgat cagctgtcag ccatcatggc aaaacttgtc gcggcagacc
    74401 ttttgatcgt cctttctgat atcgacgggt tttattcaga taatccgact acaaatcccg
    74461 aggctcagat gtatcgctat atcgataaaa tcgatgaaaa gattctggca caagcaggtg
    74521 gtgctggtag cacatttgga acaggcggta tgcagagtaa attaaaagcc gcttctagga
    74581 tatttgaagc aaacagcgca atgattttag caaatggaaa aaatccggca attatttttg
    74641 atattcttgc aggaaaagaa gtaggtactt tatttaagga ggacttgaaa tgactgactt
    74701 gatgcaatta ggacaacagg caaaagatag cgcgcaacaa ctagcactta tgcctacaaa
    74761 ccgcaaaaat gagctacttg ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat
    74821 cattgaagca aatcataaag acttagaaaa agcagtcgaa aacaatatca gtgaaacgat
    74881 gcaagaccgc ttgcagctga cacctgagcg aatcaatgcc atggctacag agatcgaaaa
    74941 aattgcttcg ttagacgatc ctattggaaa agtagatgag atgtggacga acaccgacgg
    75001 attgagaatc ggcaaaaagc gcgtgccact aggcgtgatc ggcatcattt acgaatctcg
    75061 tccaaatgtc acgacagatg cagctagtct agcattcaaa tcagggaatg ccgttatcct
    75121 tcgcggtggc aaagaagcat tcttctcgaa tcaattatta gtccaattat tgcagcaagt
    75181 actggtcagt gctggcgaat ctccttatgc catccagttt gtcgatgata cttctcatga
    75241 aactgcagga cagttgatgc gattgacaga atacttagat gtgctgattc ctcgaggcgg
    75301 agcaaaatta atccaacgag tcaaagaaca ggcaacggta cctatcattg aaacaggtac
    75361 aggaaataat cacgtatata tcgataaaga agcacagtta tctatggcag tgaacatcat
    75421 cgtcaatgca aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca
    75481 ttctgagatc gcaccttttt tcttgccagc tattgaaaaa gaactggtag aacacggggt
    75541 ctctttacgt gcggatgctc gtgcgttaga atatctagag acggctgctt tagcagaaga
    75601 cacagattgg gatactgaat atctagatta tatccttgct gtcaaagtgg tcgattcttt
    75661 ggatgaagca attgcacata tcaatcggca taatactaaa cattcagaaa cgatcgtgac
    75721 agatagttat gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt
    75781 caatgcctcc actcgtttta cagacggatc ggtatttggt tttggtgcag agattggtat
    75841 ctctactcaa aagttgcatg cccgtggtcc aatggggtta aatgaattaa cttccacaaa
    75901 atatatcatt tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa acattattcc
    75961 cgccttttgc cttttttctg taaaggttct atccaactag aaaggtgtca atatgtttga
    76021 aattttaatg atttttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt
    76081 cggtatttta tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa
    76141 atggtcgcag tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag
    76201 ttacatggtt gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc
    76261 aatcgcttat acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca
    76321 gctgaataaa aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga
    76381 gtgccttgaa gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt
    76441 cacagcctct ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc
    76501 ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc
    76561 atccatggac aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg
    76621 ttcccgatac acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc
    76681 ttccgcattg attattggca attctttgat atagccacga taaatcaacc gatcatctga
    76741 aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat
    76801 ctattatccc cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca
    76861 gtttcattat tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc
    76921 cgaataaatg gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat
    76981 gagaaactgt tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc
    77041 tcttggtaaa cggcgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata
    77101 tgagaagcta tttttccgtt ttgtttctct aatacttagg ataaaacgct gattttacaa
    77161 cctctgttaa acggtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat
    77221 atgaggaact atttttccgt ttgtttctct aatactcagg acaaaacgct gattttacaa
    77281 cctcctgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    77341 aatatgagaa gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    77401 caacctctga tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata
    77461 caacatacac tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata
    77521 tgttcaccaa tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa
    77581 ctatagctgg acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc
    77641 aacagtgtat tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg
    77701 actggcatag aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct
    77761 gctaaaattg tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa
    77821 gcaatagcca gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag
    77881 ccaatcaata aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat
    77941 gcgattagtc caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg
    78001 cctacttttc tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg
    78061 caaaacatca aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc
    78121 ccaccgacta gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt
    78181 gctttttctg tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat
    78241 aaagcattcc cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat
    78301 aatccgccaa gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc
    78361 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
    78421 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
    78481 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
    78541 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
    78601 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
    78661 caatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
    78721 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
    78781 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
    78841 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
    78901 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
    78961 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
    79021 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
    79081 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
    79141 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
    79201 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
    79261 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
    79321 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
    79381 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
    79441 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
    79501 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
    79561 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
    79621 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
    79681 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
    79741 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
    79801 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
    79861 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
    79921 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
    79981 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
    80041 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
    80101 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
    80161 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
    80221 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
    80281 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
    80341 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
    80401 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
    80461 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
    80521 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
    80581 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
    80641 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
    80701 agtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
    80761 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
    80821 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
    80881 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
    80941 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
    81001 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
    81061 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
    81121 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
    81181 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
    81241 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
    81301 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
    81361 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
    81421 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
    81481 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
    81541 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
    81601 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
    81661 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
    81721 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
    81781 acatagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
    81841 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
    81901 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
    81961 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
    82021 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
    82081 gcatcttccc tgattcgtaa gcttggttgt agatttcttc gccatattcg taacattgca
    82141 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
    82201 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
    82261 tcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
    82321 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
    82381 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
    82441 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
    82501 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctt gattttcttc
    82561 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
    82621 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
    82681 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
    82741 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
    82801 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
    82861 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
    82921 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
    82981 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
    83041 attgattctt atcaaactat agaagatagc tcttctgcag tagataattt tacaccgcca
    83101 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
    83161 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
    83221 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
    83281 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
    83341 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
    83401 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
    83461 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
    83521 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
    83581 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
    83641 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
    83701 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
    83761 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
    83821 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
    83881 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    83941 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    84001 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    84061 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    84121 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    84181 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    84241 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    84301 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    84361 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    84421 attgtatttt ctgagacagt agctgactgc aaagtacttt ctgtatcgtt ttcaattcct
    84481 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    84541 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    84601 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    84661 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttaat aaatttgctt
    84721 tttctgcata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    84781 gtcgtttgaa taactattta acaagtaatt ctctctggat attatccgtt atggattata
    84841 cttcctgtgt cgtcggacgg ataatcatgt tgctgacaga catccggtct ggcgtttcta
    84901 tcgcaaatgc tacagcagaa gctatatctt cagatgttaa tccccattct ttttgcgctt
    84961 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    85021 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    85081 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    85141 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    85201 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    85261 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    85321 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    85381 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    85441 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    85501 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    85561 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    85621 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    85681 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    85741 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    85801 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    85861 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    85921 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    85981 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    86041 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    86101 ctgctaaagg ctcttcgttt tccgcttgga tctgatggat cacttcaaag agcggatccg
    86161 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    86221 cttttatcct tactttataa cggacggctt atgatgtcta atacttataa aacatactta
    86281 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    86341 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    86401 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    86461 aacttggtgt gacacttttc actcgaggct cccgagagat acagttgacg gattctggtc
    86521 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    86581 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    86641 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    86701 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    86761 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    86821 aagatcgttg gggacttttg atacatcgga atcatccttt agcaaagcaa acagtcatcc
    86881 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    86941 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    87001 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    87061 ggatcatcaa tacttatggg acggaaaaag tattcatccc actcgatccg ccattacaag
    87121 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    87181 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    87241 taacaacgaa taagttaagc aaaagatcca atgattaaat aacataagtt tcttaatttg
    87301 ttccttcctt ttatttctga taattccagt aaattactat aattataaaa aaagaattga
    87361 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
    87421 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
    87481 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
    87541 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
    87601 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
    87661 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
    87721 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
    87781 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
    87841 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
    87901 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
    87961 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
    88021 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
    88081 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
    88141 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
    88201 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
    88261 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
    88321 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
    88381 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
    88441 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
    88501 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
    88561 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
    88621 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
    88681 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
    88741 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
    88801 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
    88861 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
    88921 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
    88981 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
    89041 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
    89101 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
    89161 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
    89221 aacaaagcaa aaacagcttc cttgatgtta gcagcagctt ttgcttcatt ctttactggt
    89281 gtaacagaac cacttgaatt ctcatttatg ttcgttgctt ggccattata tgtcttgcat
    89341 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
    89401 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
    89461 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
    89521 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
    89581 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
    89641 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
    89701 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
    89761 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
    89821 gttgcagatg agatggcaaa actacatggt ggtgctgcaa cacgacctgc acaaacaaac
    89881 accgtggtta aaacagaaac atttgccggt ggaacgatcg aacaagatat ttacgctatc
    89941 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
    90001 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
    90061 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
    90121 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
    90181 tatgtaaaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
    90241 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
    90301 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
    90361 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
    90421 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
    90481 aactccgcgg taataagtcc agccaaacgt aacatatgaa gagctatttt ttatttgact
    90541 cttcttatta cttgatgcag aacagctttt ttacaacctc tgataaacgg tgttcaaaaa
    90601 ccagtttatc ggtatgaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat
    90661 ttttcttcat actcaaaact aatcggcttt ttcacaacct ctttatacat tattcctgat
    90721 ccaatatttc ttttaatgct gcttcaagag aagttaatgg ttttcccaat actttttcta
    90781 aatcatcaga ggaaatagct aactgatcct ttttaatatc atgttggatc gctaaaaaca
    90841 tgtcagctgc ttcttgaggg aatccggctt cttttagatt ttcgacaaac gctgcatcac
    90901 ttgcttcaat aatttctaac tttttacctg tcgcacgctc taatgccttg gctaattctt
    90961 catagtcagt tagattgccg gataattcta agatttctgg agagtcactt tctactagta
    91021 cttttgctgc agcttctgca tattcacgtt tcaatgccca acctgttttt ccatttcctg
    91081 cagcataaac gaattgacca gtttttaaag cctgcccaat gataggtaat tcattctcta
    91141 cataccaatt attccgtaaa aatgtatggg aaatacctga atccttaatc attttttcag
    91201 tgaattggtg atctggcgct aatacactga tcgagtgatc ggcatctgca aaacttgtat
    91261 aagcgatata agatacccca gctgcttgtg ctgccttcac tacattctta tgttcggctt
    91321 gacgatttcc tggaacgcca gagacaaaaa gcaatcgatc tattcctttc aacgcttctt
    91381 tcattgagtc taaatcatca taatcaccaa tacgtatatt aaatccagct tctttcagtg
    91441 cttctccttt ttcttcacta cgtactagta catagatttc attttgtgga accatctttt
    91501 ttaagaattc cagcgcataa ccaccaaaac ctccagttgc tcctgttact aaataatgca
    91561 tatttgccac tccctattct ttttatttgt taagatttac agctgtacat ttataaatta
    91621 cacctttatc ataaagagtg tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa
    91681 ctgatgaagt attctctgca atttagtgat gcaatccatg tacttgccta tattgaaatg
    91741 tttcaagata cccgatcact ttctagtgag atgattgcca gtagtatcga aacaaatgca
    91801 gcaaatgtac gtaaaatcat gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc
    91861 ggaaaaccgc agccttccct tgccaaaagt ccagatcaga tttctttact agatgtctat
    91921 cgcagcatcg aaggtaatac acagcttatc caagtcgatc caaaaacaaa tccaaattgc
    91981 gtcgttggtg cgaatatcca agaagcttta gaagaaagct acgctcggct gcagaaaaag
    92041 gcagaagaag aaatggctaa gatcacactt gatagtctga tacataaaat agccgttttg
    92101 gaaaaagaaa aaagaccaga aaacacaggc ctcgtagaga gatttttata attctctcta
    92161 ctgtgcctgc atttttctga tcggaatcgt ctacataaat tcttttattc tccgggggac
    92221 aaaagccttg tcatgcccct ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg
    92281 acgtcttgat aaacggtgtt caagtagctg accctcggca ttagttctga aaaatgcaaa
    92341 atatggaaag ctatttccca tttttcctct ctaatgctca ggtcaaagcg ctactttcac
    92401 gacctctata ttatcatggt ttattgacta ccggtatcca gatttctccc atatatttcc
    92461 cgttggtaac tcccatttcg actacagcat ttggaccccc aacataggcg taatcttcta
    92521 cttctcttaa aacatgccca aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg
    92581 attctgctaa agcgattaca acaagatacg ttccttttgg gaatgggatg attcgggtag
    92641 tgtttggaac atcctgactc gtttgaacac cgacatagta catcattttc ccgttatagg
    92701 gttcgttgac tgtaaagaaa taatgatttt cagcagcttg tttcaatgca tctattgttc
    92761 cattaccgat aactttttgc tcaaatgctt gtttctctgc agccaaaccg acaaaatccg
    92821 tattgctact cttcaattcc acaccaatac ccgtcaccgt aaaatctggt ttttcttcga
    92881 tcctatactt tgccatttct cccacccttt ctattatatt aagttcatct tacataaaat
    92941 taaagggaaa tgacacgatt acgctctttt ttctagggat atttttattt tttaactatt
    93001 tttttgcttt cactgcaatc caaatctcac tgtaaacatt actgaaatcc tctccccatt
    93061 ttgtaaaaga gatctcggga gcttcgacta actcataatc tgaagaagga agccattctg
    93121 catagatttt cccccaagta tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa
    93181 tggcccaagt gtgttcttct atagcaagca tttctaaatc agcgtacgga ttttcttttg
    93241 tcgtagcaaa gccaatcatg tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa
    93301 atccataaga aacgttgatt acttgatttg gatataaatc tcctaactta tgcatctctt
    93361 ccctttgagt ctccgtgatc gtctgcgcta acttttgtat ttcttgattg actccttcaa
    93421 atttgacagg aacttttttc gccacacctg ccacataaaa ctgttctttc ttttcgatac
    93481 gaaattccat cgattttcct cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt
    93541 tttgtatgcc gtatctgctg atttctgaag gcagatatcc tgaccaatca cgaaaagctc
    93601 ttgaaaaacc ttcgactgtt tcatatccat atctaaacgc cgtttctgtc actcttgatc
    93661 catctaggag atcgcgatta gccaaagcca atctacggtt tttgatatag tccattagcg
    93721 acatccctgc aataaacgta aagatgcgtt ttaattgata ttcagagatt cctgtatttt
    93781 cggctattat tttataggaa agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt
    93841 ggttcagatc tttaagcata tttatcttcc tccttgcttg cctttttatc atattatcaa
    93901 agaaagtcaa ttacttgatg aatcaggaac caatttagtc agatgtagaa aatgttgtat
    93961 ggatctaaag aaaattctca agcagcgttt cgtaacggat aattggtatt atctgcctat
    94021 ctatgtttta cctttagaaa aataaatttt ggggaagaca cattctaagt aaacaaagcg
    94081 gtaaaaatat cttttattcc cttagttaat ccgcttacta cctactgtac aaagcaaatt
    94141 gttgttcaaa ctgctgatca gaacgctgct tggatttttc tttgcgatac atcatacctc
    94201 ttttatcaat tggaacaatt atttatttct aacaaaatca cttcattttt gtcagataaa
    94261 taaa
//
LOCUS       DARDHU010000003        85694 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000003 DARDHU010000000
VERSION     DARDHU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.003"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            213..731
                     /locus_tag="SDD03_000230"
     CDS             213..731
                     /locus_tag="SDD03_000230"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4302711.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            859..1542
                     /locus_tag="SDD03_000231"
     CDS             859..1542
                     /locus_tag="SDD03_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG4302712.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1851..1924
                     /locus_tag="SDD03_000232"
     tRNA            1851..1924
                     /locus_tag="SDD03_000232"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1885..1887,aa:Arg,seq:tct)
     gene            2083..2442
                     /locus_tag="SDD03_000233"
     CDS             2083..2442
                     /locus_tag="SDD03_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG4302713.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2565..4196
                     /locus_tag="SDD03_000234"
     CDS             2565..4196
                     /locus_tag="SDD03_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG4302714.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4258..4899)
                     /locus_tag="SDD03_000235"
     CDS             complement(4258..4899)
                     /locus_tag="SDD03_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG4302715.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5085..5161
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5181..5489
                     /gene="rplU"
                     /locus_tag="SDD03_000236"
     CDS             5181..5489
                     /gene="rplU"
                     /locus_tag="SDD03_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG4302716.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5516..5860
                     /locus_tag="SDD03_000237"
     CDS             5516..5860
                     /locus_tag="SDD03_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG4302717.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5873..6166
                     /gene="rpmA"
                     /locus_tag="SDD03_000238"
     CDS             5873..6166
                     /gene="rpmA"
                     /locus_tag="SDD03_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG4302718.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6600..9407
                     /locus_tag="SDD03_000239"
     CDS             6600..9407
                     /locus_tag="SDD03_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4302719.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9598..10089
                     /locus_tag="SDD03_000240"
     CDS             9598..10089
                     /locus_tag="SDD03_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4302720.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10192..11181
                     /gene="manX"
                     /locus_tag="SDD03_000241"
     CDS             10192..11181
                     /gene="manX"
                     /locus_tag="SDD03_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG4302721.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11214..12017
                     /locus_tag="SDD03_000242"
     CDS             11214..12017
                     /locus_tag="SDD03_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG4302722.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12038..12952
                     /locus_tag="SDD03_000243"
     CDS             12038..12952
                     /locus_tag="SDD03_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG4302723.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13035..13409
                     /locus_tag="SDD03_000244"
     CDS             13035..13409
                     /locus_tag="SDD03_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG4302724.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13548..13793
                     /locus_tag="SDD03_000245"
     CDS             13548..13793
                     /locus_tag="SDD03_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4302725.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13965..15029
                     /locus_tag="SDD03_000246"
     CDS             13965..15029
                     /locus_tag="SDD03_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG4302726.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15275..15700
                     /locus_tag="SDD03_000247"
     CDS             15275..15700
                     /locus_tag="SDD03_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4302727.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15693..16142
                     /gene="nusB"
                     /locus_tag="SDD03_000248"
     CDS             15693..16142
                     /gene="nusB"
                     /locus_tag="SDD03_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG4302728.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16239..17093
                     /locus_tag="SDD03_000249"
     CDS             16239..17093
                     /locus_tag="SDD03_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG4302729.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17094..18431
                     /locus_tag="SDD03_000250"
     CDS             17094..18431
                     /locus_tag="SDD03_000250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG4302730.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18436..18660
                     /locus_tag="SDD03_000251"
     CDS             18436..18660
                     /locus_tag="SDD03_000251"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG4302731.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18662..19552
                     /locus_tag="SDD03_000252"
     CDS             18662..19552
                     /locus_tag="SDD03_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG4302732.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19561..20379
                     /locus_tag="SDD03_000253"
     CDS             19561..20379
                     /locus_tag="SDD03_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG4302733.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20486..20935
                     /locus_tag="SDD03_000254"
     CDS             20486..20935
                     /locus_tag="SDD03_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG4302734.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20953..22635
                     /gene="recN"
                     /locus_tag="SDD03_000255"
     CDS             20953..22635
                     /gene="recN"
                     /locus_tag="SDD03_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG4302735.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22690..22806)
                     /locus_tag="SDD03_000256"
     CDS             complement(22690..22806)
                     /locus_tag="SDD03_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG4302736.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22879..23835)
                     /locus_tag="SDD03_000257"
     CDS             complement(22879..23835)
                     /locus_tag="SDD03_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4302737.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            24006..24374
                     /locus_tag="SDD03_000258"
     CDS             24006..24374
                     /locus_tag="SDD03_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG4302738.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24544..24975
                     /gene="mraZ"
                     /locus_tag="SDD03_000259"
     CDS             24544..24975
                     /gene="mraZ"
                     /locus_tag="SDD03_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG4302739.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24996..25955
                     /gene="rsmH"
                     /locus_tag="SDD03_000260"
     CDS             24996..25955
                     /gene="rsmH"
                     /locus_tag="SDD03_000260"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG4302740.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25960..26361
                     /gene="ftsL"
                     /locus_tag="SDD03_000261"
     CDS             25960..26361
                     /gene="ftsL"
                     /locus_tag="SDD03_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG4302741.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26362..28554
                     /locus_tag="SDD03_000262"
     CDS             26362..28554
                     /locus_tag="SDD03_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG4302742.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28580..29542
                     /locus_tag="SDD03_000263"
     CDS             28580..29542
                     /locus_tag="SDD03_000263"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG4302743.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29556..30917
                     /locus_tag="SDD03_000264"
     CDS             29556..30917
                     /locus_tag="SDD03_000264"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG4302744.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30943..32031
                     /gene="murG"
                     /locus_tag="SDD03_000265"
     CDS             30943..32031
                     /gene="murG"
                     /locus_tag="SDD03_000265"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG4302745.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32046..33266
                     /locus_tag="SDD03_000266"
     CDS             32046..33266
                     /locus_tag="SDD03_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG4302746.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33422..34747
                     /gene="ftsA"
                     /locus_tag="SDD03_000267"
     CDS             33422..34747
                     /gene="ftsA"
                     /locus_tag="SDD03_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG4302747.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34770..36011
                     /gene="ftsZ"
                     /locus_tag="SDD03_000268"
     CDS             34770..36011
                     /gene="ftsZ"
                     /locus_tag="SDD03_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG4302748.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36028..36705
                     /locus_tag="SDD03_000269"
     CDS             36028..36705
                     /locus_tag="SDD03_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG4302749.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36719..37318
                     /locus_tag="SDD03_000270"
     CDS             36719..37318
                     /locus_tag="SDD03_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG4302750.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37333..37620
                     /locus_tag="SDD03_000271"
     CDS             37333..37620
                     /locus_tag="SDD03_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG4302751.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37669..38451
                     /locus_tag="SDD03_000272"
     CDS             37669..38451
                     /locus_tag="SDD03_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG4302752.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38472..39179
                     /locus_tag="SDD03_000273"
     CDS             38472..39179
                     /locus_tag="SDD03_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG4302753.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39441..42239
                     /gene="ileS"
                     /locus_tag="SDD03_000274"
     CDS             39441..42239
                     /gene="ileS"
                     /locus_tag="SDD03_000274"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG4302754.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42288..43814)
                     /locus_tag="SDD03_000275"
     CDS             complement(42288..43814)
                     /locus_tag="SDD03_000275"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG4302755.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43829..44476)
                     /locus_tag="SDD03_000276"
     CDS             complement(43829..44476)
                     /locus_tag="SDD03_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG4302756.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44660..44989)
                     /locus_tag="SDD03_000277"
     CDS             complement(44660..44989)
                     /locus_tag="SDD03_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG4302757.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45166..45894
                     /gene="sfsA"
                     /locus_tag="SDD03_000278"
     CDS             45166..45894
                     /gene="sfsA"
                     /locus_tag="SDD03_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG4302758.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45910..46923
                     /locus_tag="SDD03_000279"
     CDS             45910..46923
                     /locus_tag="SDD03_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4302759.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46923..48200
                     /locus_tag="SDD03_000280"
     CDS             46923..48200
                     /locus_tag="SDD03_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4302760.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48263..50965
                     /locus_tag="SDD03_000281"
     CDS             48263..50965
                     /locus_tag="SDD03_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4302761.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51117..51434
                     /locus_tag="SDD03_000282"
     CDS             51117..51434
                     /locus_tag="SDD03_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4302762.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51464..51784
                     /locus_tag="SDD03_000283"
     CDS             51464..51784
                     /locus_tag="SDD03_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4302763.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51892..53352
                     /locus_tag="SDD03_000284"
     CDS             51892..53352
                     /locus_tag="SDD03_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4302764.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53420..53641
                     /locus_tag="SDD03_000285"
     CDS             53420..53641
                     /locus_tag="SDD03_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302765.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53672..53854
                     /locus_tag="SDD03_000286"
     CDS             53672..53854
                     /locus_tag="SDD03_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG4302766.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53854..54267
                     /locus_tag="SDD03_000287"
     CDS             53854..54267
                     /locus_tag="SDD03_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG4302767.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54390..55571
                     /locus_tag="SDD03_000288"
     CDS             54390..55571
                     /locus_tag="SDD03_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4302768.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55642..55806)
                     /locus_tag="SDD03_000289"
     CDS             complement(55642..55806)
                     /locus_tag="SDD03_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302769.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55960..56033
                     /locus_tag="SDD03_000290"
     tRNA            55960..56033
                     /locus_tag="SDD03_000290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55994..55996,aa:Arg,seq:cct)
     gene            complement(56070..56270)
                     /locus_tag="SDD03_000291"
     CDS             complement(56070..56270)
                     /locus_tag="SDD03_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4302770.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56575..57807)
                     /locus_tag="SDD03_000292"
     CDS             complement(56575..57807)
                     /locus_tag="SDD03_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG4302771.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            58061..58630
                     /locus_tag="SDD03_000293"
     CDS             58061..58630
                     /locus_tag="SDD03_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4302772.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58808..59248)
                     /locus_tag="SDD03_000294"
     CDS             complement(58808..59248)
                     /locus_tag="SDD03_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG4302773.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59406..60170
                     /gene="map"
                     /locus_tag="SDD03_000295"
     CDS             59406..60170
                     /gene="map"
                     /locus_tag="SDD03_000295"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG4302774.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60202..61125
                     /locus_tag="SDD03_000296"
     CDS             60202..61125
                     /locus_tag="SDD03_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG4302775.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61201..62340
                     /locus_tag="SDD03_000297"
     CDS             61201..62340
                     /locus_tag="SDD03_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG4302776.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62333..63133
                     /locus_tag="SDD03_000298"
     CDS             62333..63133
                     /locus_tag="SDD03_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4302777.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            63133..63960
                     /locus_tag="SDD03_000299"
     CDS             63133..63960
                     /locus_tag="SDD03_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4302778.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63938..64672
                     /locus_tag="SDD03_000300"
     CDS             63938..64672
                     /locus_tag="SDD03_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4302779.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64772..65638
                     /gene="rfbA"
                     /locus_tag="SDD03_000301"
     CDS             64772..65638
                     /gene="rfbA"
                     /locus_tag="SDD03_000301"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG4302780.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65652..66224
                     /gene="rfbC"
                     /locus_tag="SDD03_000302"
     CDS             65652..66224
                     /gene="rfbC"
                     /locus_tag="SDD03_000302"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG4302781.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66246..67274
                     /gene="rfbB"
                     /locus_tag="SDD03_000303"
     CDS             66246..67274
                     /gene="rfbB"
                     /locus_tag="SDD03_000303"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG4302782.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67372..68223
                     /gene="rfbD"
                     /locus_tag="SDD03_000304"
     CDS             67372..68223
                     /gene="rfbD"
                     /locus_tag="SDD03_000304"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG4302783.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68258..70291
                     /locus_tag="SDD03_000305"
     CDS             68258..70291
                     /locus_tag="SDD03_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302784.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70335..71615
                     /locus_tag="SDD03_000306"
     CDS             70335..71615
                     /locus_tag="SDD03_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG4302785.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71825..72631
                     /locus_tag="SDD03_000307"
     CDS             71825..72631
                     /locus_tag="SDD03_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4302786.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72643..73863
                     /locus_tag="SDD03_000308"
     CDS             72643..73863
                     /locus_tag="SDD03_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4302787.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73853..75439
                     /locus_tag="SDD03_000309"
     CDS             73853..75439
                     /locus_tag="SDD03_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4302788.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75478..77616
                     /locus_tag="SDD03_000310"
     CDS             75478..77616
                     /locus_tag="SDD03_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4302789.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77984..78967)
                     /locus_tag="SDD03_000311"
     CDS             complement(77984..78967)
                     /locus_tag="SDD03_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG4302790.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79345..80736
                     /locus_tag="SDD03_000312"
     CDS             79345..80736
                     /locus_tag="SDD03_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG4302791.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80752..81792
                     /locus_tag="SDD03_000313"
     CDS             80752..81792
                     /locus_tag="SDD03_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4302792.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81811..82896
                     /locus_tag="SDD03_000314"
     CDS             81811..82896
                     /locus_tag="SDD03_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG4302793.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            82929..83891
                     /locus_tag="SDD03_000315"
     CDS             82929..83891
                     /locus_tag="SDD03_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG4302794.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            83884..85311
                     /locus_tag="SDD03_000316"
     CDS             83884..85311
                     /locus_tag="SDD03_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG4302795.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            85313..>85694
                     /locus_tag="SDD03_000317"
     CDS             85313..>85694
                     /locus_tag="SDD03_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302796.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTY"
BASE COUNT        28117 a        14260 c        17987 g        25330 t
ORIGIN      
        1 taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa actaacgtca
       61 attatttata ataaaaaatg agataaagat gaggaaatag aattagaaac atataattat
      121 taaagggaaa catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa
      181 gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta
      241 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
      301 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
      361 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
      421 ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa
      481 acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag
      541 taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc
      601 tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa
      661 caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga
      721 aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa
      781 catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt
      841 gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa
      901 ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa
      961 gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa
     1021 tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt
     1081 tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt
     1141 ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa
     1201 gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac
     1261 tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt
     1321 ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg
     1381 ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt
     1441 cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt
     1501 gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc
     1561 tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt
     1621 gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta
     1681 gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga
     1741 ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat
     1801 tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag
     1861 ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt
     1921 gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca
     1981 atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata
     2041 aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga
     2101 attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga
     2161 actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa
     2221 tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta
     2281 ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct
     2341 attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa
     2401 ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa
     2461 tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta
     2521 atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg
     2581 tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt
     2641 tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa
     2701 gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg
     2761 gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga
     2821 tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc
     2881 tatttttact tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac
     2941 ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata
     3001 atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa
     3061 cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga
     3121 tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt
     3181 tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac
     3241 aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac
     3301 aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg
     3361 gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc
     3421 aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg
     3481 cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg
     3541 cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc
     3601 cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca
     3661 aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt
     3721 gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat
     3781 tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa
     3841 tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac
     3901 tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg
     3961 gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg
     4021 atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc
     4081 cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa
     4141 cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca
     4201 atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta
     4261 atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga
     4321 gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga
     4381 ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc
     4441 atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag
     4501 tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc
     4561 aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg
     4621 tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac
     4681 ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc
     4741 gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag
     4801 tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct
     4861 taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt
     4921 tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag
     4981 aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta
     5041 acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc
     5101 tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc
     5161 agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag
     5221 ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg
     5281 tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg
     5341 caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt
     5401 tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa
     5461 aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat
     5521 caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca
     5581 tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat
     5641 cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga
     5701 agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa
     5761 tattgctgag attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa
     5821 ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt
     5881 atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg
     5941 tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg
     6001 atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg
     6061 tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg
     6121 taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat
     6181 taaagaggtt gtgacaagac ttttgtcaca gccttttttg tttataaaaa taaaagttgt
     6241 agtggaggct acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga
     6301 atcaggatgt tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag
     6361 tgaaataatt atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg
     6421 ggatttttcg ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa
     6481 aatcaggtaa atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa
     6541 gtacaatagt ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata
     6601 tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa
     6661 cacaggaaat tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc
     6721 agctagtgcg cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc
     6781 aaacactcgt tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc
     6841 cggtctctga agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca
     6901 ttggagctaa cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc
     6961 cgccaagagg attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg
     7021 cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga
     7081 ttgttttcaa ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg
     7141 ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag
     7201 cggatggcgg tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa
     7261 tgatctttta ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc
     7321 atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag
     7381 aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg
     7441 caaatgagaa agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc
     7501 gaaagatttc tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa
     7561 atataggaca attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata
     7621 tgcaatcacc cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat
     7681 tgattcaatt agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa
     7741 agataactgt agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac
     7801 cttataatct atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg
     7861 atcaagaagc aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct
     7921 ataaagacca tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga
     7981 aaatcataga agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt
     8041 ttcagcaaaa ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca
     8101 acttaggaga agaacggcat acgaatgata acatccgtga gatggctttt gattttccag
     8161 atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga
     8221 ttccagaaag tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag
     8281 caagcggcag aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg
     8341 tccaagttgt ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg
     8401 atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag
     8461 gaagcggggt catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc
     8521 aaagagacac tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg
     8581 aaactgtacg aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga
     8641 aaaattttcg aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta
     8701 ttcataagaa aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa
     8761 tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag
     8821 ctttgtcgat tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca
     8881 tagctactac tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt
     8941 ttattgatca aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg
     9001 aaactgaatt catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct
     9061 ctgataactt catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta
     9121 cacaaatctc tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag
     9181 tgatgcacac agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag
     9241 aaaacgaatt gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg
     9301 ttctgttgga agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac
     9361 gtctagtcca taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg
     9421 atacactttt atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag
     9481 tgtatcgtaa aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg
     9541 ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg
     9601 gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg
     9661 tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa
     9721 ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc
     9781 gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt
     9841 ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg
     9901 ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca
     9961 gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag
    10021 attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa
    10081 aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa
    10141 ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg
    10201 attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc
    10261 tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac
    10321 ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc
    10381 ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac
    10441 aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca
    10501 tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa
    10561 gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt
    10621 gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc
    10681 aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg
    10741 acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat
    10801 ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct
    10861 attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt
    10921 ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa
    10981 ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct
    11041 atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat
    11101 gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa
    11161 gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta
    11221 ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg
    11281 atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta
    11341 acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa
    11401 atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt
    11461 tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt
    11521 tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc
    11581 atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca
    11641 ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc
    11701 cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg
    11761 ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta
    11821 caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa
    11881 cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta
    11941 aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca
    12001 ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt
    12061 atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa
    12121 ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt
    12181 atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac
    12241 acacccatac attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc
    12301 gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc
    12361 tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact
    12421 aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg
    12481 gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc
    12541 taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat
    12601 tttagggatg ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattcttacc
    12661 agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc
    12721 aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc
    12781 tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt
    12841 aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat
    12901 ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa
    12961 aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata
    13021 tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta
    13081 cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtgataat ggttttgagt
    13141 tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg
    13201 tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa
    13261 aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag
    13321 aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg
    13381 gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt
    13441 ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct
    13501 ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat
    13561 ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa
    13621 attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag
    13681 cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag
    13741 gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta
    13801 caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat
    13861 ttaaaataaa aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt
    13921 tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag
    13981 aaaaattgcg caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt
    14041 acaatcttcg ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg
    14101 aaaaagcatt ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag
    14161 ggtttgaaat tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca
    14221 aagaagaact gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg
    14281 ttttagaaga aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca
    14341 gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata
    14401 tggcatatga tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa
    14461 atcaactaga cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg
    14521 ttgctagcgg attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac
    14581 aaggtgactt gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga
    14641 cacgtacatt cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct
    14701 tagatgctca attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag
    14761 atgcagttgc tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta
    14821 caggacatgg gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa
    14881 agcaatttgt accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg
    14941 gtggagtacg tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc
    15001 gttcaccaaa agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat
    15061 cagtgttcac agactttgtt atagaaaatc actcgattta atgatacact atagaagaac
    15121 ttttaagctg taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc
    15181 tgatcggcgg cagttttctt accacgtgat cactatgccg catcatagag aattcggatg
    15241 tatctcgtgc ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt
    15301 aaacgcaaac caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat
    15361 cgggattgca gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa
    15421 tgtcactgaa ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga
    15481 agatggcttg aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt
    15541 agcaatggaa atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt
    15601 atctgaagtc aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat
    15661 cgaagaactt tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga
    15721 ttcgggaaat ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag
    15781 aagatgcgat tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat
    15841 cagagtttgt tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg
    15901 aattggataa agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa
    15961 aaatggattt ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac
    16021 cagcggctgt tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt
    16081 cacgtaaatt cgtcaacggt gttttatcca atgtattgaa agaattagaa gctggggcgt
    16141 aagtcctagc ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg
    16201 gtagaatgaa aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa
    16261 aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata
    16321 atggtatcca tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct
    16381 atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt
    16441 atcctgagac gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc
    16501 ctagttttca tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga
    16561 tcctgttagc tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca
    16621 gactccttgc gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt
    16681 ttgaagctta ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata
    16741 tcgtcggtaa accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc
    16801 attctaaaac cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa
    16861 taggacgtgg acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg
    16921 tagggatgaa tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg
    16981 agcctattgc aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga
    17041 tgctgatgta tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac
    17101 aagaatattt aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc
    17161 cttatttaga acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg
    17221 cccatcaata tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag
    17281 gagcattcca aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc
    17341 gtatttcatt atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg
    17401 atggaatagg tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag
    17461 gcctttttca agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta
    17521 ccagtccaag cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa
    17581 ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg
    17641 tcaggaatat ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg
    17701 ggggtggttc gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt
    17761 atcaagctaa tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg
    17821 atttagtagc agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg
    17881 ttttgtcaga agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg
    17941 tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc
    18001 ggcagcctga aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat
    18061 tatatcaagc tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc
    18121 accaattgga acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt
    18181 atctagaaaa acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt
    18241 ttgctcaagc tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct
    18301 acagctatac gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg
    18361 atttgctgac gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca
    18421 aaggagaata aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa
    18481 attgtcatgc agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa
    18541 cgaggaatgg aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg
    18601 acgaaaatga tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga
    18661 gatgagatta actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc
    18721 ttttttagat gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa
    18781 agcaggcggg aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga
    18841 gcctattgat gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc
    18901 cttgatccac gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac
    18961 caatcataaa gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg
    19021 agcattccag ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca
    19081 gcaattagct gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga
    19141 gggcgaatcg aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg
    19201 gcatttgatt cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat
    19261 cttattgcta cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga
    19321 tctactcgat gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg
    19381 tatggaaaaa aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga
    19441 gattgagctg ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt
    19501 tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga
    19561 atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa
    19621 caagccaaac gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat
    19681 aaaccgggag aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct
    19741 tatgtttctc gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt
    19801 gaaggaaaaa cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg
    19861 caaaatggcg caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa
    19921 ataagacaag atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc
    19981 gaagacttta ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta
    20041 aaactgatgc tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta
    20101 atcaaacccc aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac
    20161 cctcagaccc atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat
    20221 gatgtgctgg aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg
    20281 gctcacttga ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa
    20341 gtggtttcaa atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag
    20401 tgagaagtat gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa
    20461 gaatatgcta aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg
    20521 attgttgagt gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa
    20581 aggaatatct gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa
    20641 ggtaccggct tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt
    20701 aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa
    20761 atttgttatt ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa
    20821 acgttacaag aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac
    20881 acgaactgaa gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga
    20941 ggagcgaatg tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg
    21001 cggctttctt ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc
    21061 attattatcg atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt
    21121 caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc
    21181 tctgaactga tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg
    21241 gatatgtcct taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct
    21301 aatctgagaa gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa
    21361 ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa
    21421 cagaagaaaa agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta
    21481 cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag
    21541 caagaagaaa tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa
    21601 agagaaaaac ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca
    21661 ctgacgaata atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa
    21721 gggatcgccc atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac
    21781 tatttgctgc aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat
    21841 gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa
    21901 tacggcgaat ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct
    21961 tcttctgatt tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag
    22021 cttctacttc agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa
    22081 ttggaaaaat ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag
    22141 gtacgtttct taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa
    22201 ttttacatta cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt
    22261 ggcgaactgt ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg
    22321 acaagtatta tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc
    22381 gcagataaaa tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct
    22441 caagttgctg cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt
    22501 acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg
    22561 ctggctggaa gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc
    22621 gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat
    22681 attttatttt tacatgacac tcattacagc agtcaataca acagatccaa tcgttaaaat
    22741 cagcattgtc caaatgacaa attttgttac tttagcaaaa gtacttgttt gttttttttc
    22801 acgcattgtt ttcaacctcg tcattaatta gttgattcaa gtgtacaatc ttttttccat
    22861 ttccacaaga gggctttttt ataaaaactt cccttttttt aaataatagg tagttacgat
    22921 acaaataaat aatgttaata gaactagcca tataaaggca tcatcccttt cagcaatagg
    22981 taacttaatg ttcattccat agatactgcc gacgattgtc ggtatagtta aaataatcgt
    23041 taaagaagtt aaaattttca taatattatt cagattattc gatacgattg ctgaaaaagt
    23101 ctgattcatt tgttctacta gttttaattg aatgcgagaa gtagtcattg cctgctctgt
    23161 ttccaccaat acatcacgta aatgattttt caaagcatga ttttcttgga aaagtttatt
    23221 ttgcgctagt tctttcaatg catctaaatt agcagaagtc gcttctctga aataaacgag
    23281 gcttttttgg atatccatga tttgataaag ttgtttgttt tcagtggata cttgaagttt
    23341 ggcttccaaa gtatcgcatt gagattgaag cgtagctaaa taactgttgt aagagagtgt
    23401 gacctgccaa atgaattgaa agaagatatt gagattaggc gaaatatcac tttttggaaa
    23461 ggtctggcta aagactttct ttaaaaacag tggttcatga ttcgtaattg tgataagctt
    23521 ctttttgggc gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat aaccgctagg
    23581 acttgtttta gcaaaaggat attgtaacaa taataacgct ggctttgtca actttgattg
    23641 attgaattct tctgtacggg aattttctgc atcgtccaat atggaagtga tgtaatctga
    23701 cggaatttca aattgatgtg tcagctgatg aatctcttct tgattaggac gttctacaca
    23761 ataccagatc gtttggtcag tttcctttcc ggtattacgc agtttctcat tatctaatgt
    23821 aaaatatttg atcatgattt tcctcctgtt gcctgtttct tttattataa cggataggga
    23881 attggaatac atggaatgtg tttgtaagat atagaatata actgcataaa tttaaataaa
    23941 atgaaacagt tgtgtaaagt atatacagta tacttgaagc aacgataaaa aaggagttta
    24001 cgttcatggg cacattcaca ccacttatct tgttttggta tatttacccg gtaatcgttt
    24061 tgtttggttg ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag
    24121 cccctgattt ggcgattcct tttttgtggg gcggtatgca tgcattgtct agaaatacag
    24181 ggacaatttc tatcttaccg tttttcttta tttctgtttt gctaatggga atttgtattg
    24241 ctgttttcca agcatattat tatgaagaaa ttatttatcc gcgttatttc aagatgactt
    24301 ggcgtttggt ttttctgttg acaatcgtct tgtacgctgt cttgatcatt gtgaacatac
    24361 tttcttacct atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag
    24421 cttgattttt aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt cccagaaaag
    24481 gtggtagaaa gtgggggatt gtggtagact aattttagtt agtggaggaa tggggggctt
    24541 tcaatgttga tgggtgaatt ccaacataat attgatgcaa aaggacgttt aatcgtgccc
    24601 tcaaaacttc gtgaagaact tggcgaaaag tttgttctga cacgcggatt ggatggctgt
    24661 cttttcggct atccgatgag cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg
    24721 ttggctaaaa aagacgcacg tacttttgtg cgattcttct attcggctgc cactgaatgc
    24781 gaacttgata aacaaggacg aatcaatata cccagcacgt taagaaatta tgcagctttg
    24841 acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg aaatttggga tgagacacga
    24901 tggcaagatt tttctgcagc agcagaagag aactttgacg aaattgctga aagtatgatt
    24961 gatttcggac tataagaata ggaagaggct gactaatgac tgaaacattt caacactata
    25021 cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa accggatggc gtttatgtag
    25081 actgtacact cggcggtgcc ggacatagtg aatatttatt aagccaacta agttctaaag
    25141 ggcatctgta tgcgtttgac caagatcaaa aagcaatcga tcacgccaag ctgcgtttag
    25201 cttcttatgt cgaacaaggt atggtcacat ttatcaaagc gaacttcaga gaattagaag
    25261 aacgcttgag tgagcatgta gataaggtag acggcatttt gtatgatcta ggggtttcct
    25321 ctcctcagct agatgaagca gaacgtggat ttagttatca tcaagatgca ccgttggata
    25381 tgcgcatgga tcagtcagct cctttatccg cttatcacgt aattaatgaa tattcctacc
    25441 atgaattagt aaagattttc ttccgctatg gcgaggagaa attttccaaa caaattgctc
    25501 gtgaaatcga acgagtacga aaaacaaaac caatccaaac aactggtgag cttgtcgaaa
    25561 tcattaaaca agtcatccca gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa
    25621 tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag gcctctttag
    25681 aacaggcaat ccggttattg aaagtcaatg gaagaatcag cgtaatcaca ttccattcat
    25741 tggaagacag aattgtcaaa agtatgttta aagaatatag tacggtacaa gatctaccac
    25801 cgggacttcc tgtcatacct gacgaattcc aaccagaact gaaagtcatt actcgtaaac
    25861 caatccttcc ttcagaggaa gagctaacag aaaacaatcg atcacgcagt gcaaagttac
    25921 gtattgcaga aaaaataaga atgaaagagg agtagaatga tggcagaatt aaagaaaatg
    25981 caagattacg aatatgattt acctgaattg gtggatcagc cggaacaaca aatgcctgat
    26041 tcttcttcga atcaagaaat cattcttcca caatcaccgg caaaacgatt aaagcagatt
    26101 tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt
    26161 ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa
    26221 acagaaatca atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt
    26281 tctcgtactg aacggattaa acaaatcgca gaagaaaaag gattgtctat taatgacgac
    26341 aatttaagga aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag
    26401 aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta tcattttatt tgctacgagt
    26461 attggattgt tctttttatt tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt
    26521 gctggtacct cattagctga aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa
    26581 gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct
    26641 tattcaatta aagcgatact ctcaaaaaca tatacctctg gggataaaaa actgtatgtt
    26701 gaagaaaaga attttgataa aatagcagaa atcctgcata agaatctttc tatagacaaa
    26761 aaagatgcac tgaatatttt agaggacggc gcaaaaaaag agctgtatca agttgaattt
    26821 ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa
    26881 aaagaaggtg tcgctggtct ttattttgtc gaccatcagg ctcggatgta tccaaatggc
    26941 gtcttttctt ctcattttat tggatacgca gttccagaca aagatgagaa tggcttagtt
    27001 ggtaaactag gtttggagtc tgcttacaat gatatcttga gcggtaaaga tggtaagatt
    27061 atttatcaaa aagataattt tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa
    27121 gctgtcgacg gacaagatat ctacacgact ttagacagca gactacaaag ttatttagaa
    27181 acactgatgg atcaagtaaa tgaagaatat cagccagaag aattgacagc agtcctgatg
    27241 aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac ctactttcaa tcctgaaaca
    27301 atggaaggat tgactggaaa agatgctatc tggcgtaatt tcttggtaca agatagttat
    27361 gaaccaggat caaccatgaa agttttcact actgcagctg ctatcgaaga aggagaattc
    27421 aacgagaatg aaaccttcca atctggaaaa atccaagtag aagatgctac gatcaatgac
    27481 catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac
    27541 gtcgggatgg tcatactgga acagcgatta ggcggacgat ggtataacta tttacaaaaa
    27601 ctgggatttg gacaaagtac tcattctgga ttagatgatg aagtaaacgg tgctttacct
    27661 acattaaata ttgtcgaccg agcaatgagt gcttatggac aagcggtagg tgtgacgaat
    27721 ttccaaatga tgaaagcttt cacttctatt gcgaataacg gtacaatgat tcaacctcga
    27781 tatatcagca aagtggtgga tccccaaact ggagaagagc gaacaactca aacagaagtt
    27841 ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt
    27901 gtcgaaagcg aaaactacgg tagtgcttat ggtgtatata gtgtacctgg atacaatgta
    27961 tcggcaaaaa acggaacagc ccaaatcgct tctgatacag ggggatatca aacaggagat
    28021 acagcctacc tgtattcgat tgtggagatg gttccttctg aagatccaga ctatgtcttg
    28081 tatctaacaa tgaaacaccc taagacatat gatcgaatgg cattggctaa aatcgcaaat
    28141 ccattgatga aacgggcaat ggattttaaa gaaactgaag aagattcaga tacagaaaca
    28201 aaaacagaaa aagtatccgt agccgattat cgtaatcttg aagcggacgt tgcagcagct
    28261 gatgctcaaa aaagtggact gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa
    28321 caatctactg ccaacggtga tcagttgatc tctggagaaa aactgatcct atatactggt
    28381 ggtgataagc tgatgcctga cgtaacagga tggtcaaaag cagatatcat gaagctgggg
    28441 aaaatcctcg gtattgaagt tagttttgat ggagatggtt attgtgtcaa acaagaactt
    28501 gctccatatg aaaaaatcac tgaagacaaa cttagtttta ctttagaaga ataagaagag
    28561 aaataggaga gaaaagagca tggaatggac acaagcattg attcccattg tcagcagttg
    28621 tgcaatgaca attgccgcaa tgccgctatt tattggttat tttcaaatga aaaagcaagg
    28681 tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa ctcctacaat
    28741 gggcggctta gtgtttctga tcggctctat tttgacagga atttgggttg gagcttggca
    28801 aaaacagcta actcctactc ttttcatctt gctttttgta ttagcgttat acggagtaat
    28861 cggctttctt gatgatttta taaaaatatt caagaaaaga aacatgggac tgaactcgaa
    28921 gcagaaattg attggacaga ttctaggggg aatcatcttt tatcttgttt accgttctga
    28981 aggttatcca ggtgtattga atttcttcgg actagatcta cctttaggaa tcatttatgg
    29041 tattttcgct ctcttctggc ttgttggatt ttcaaatgca gtaaatctga cagatggcat
    29101 cgatggatta gttgctggct taggaagtat ctcttttgca gcttacggtc taattgcttg
    29161 gcatcagcat cagtatgatg tattaatcat ttgcttaagt gttttaggag gtttactggg
    29221 tttcttcgtc tacaacagaa aacctgccaa gatttttatg ggagatgtcg gttcattagc
    29281 gttgggcgga cttttagcgg caatttctat aatgctaaac caagaatgga cattgctttt
    29341 gattggtttg atctatgtta tggaaacagc aagtgtgatg cttcaagtta cttcctttaa
    29401 gcttacagga aaacgaattt ttaaaatgtc tccaattcat catcactttg aaatgtgcgg
    29461 atggtcggag tggaagattg atacagtttt ttggttgatc agcattgtga cttcgttgat
    29521 cacattatgg atcgtttggt aggagatgac gggaaatgaa aaaaatatca acatatgaaa
    29581 ataagaaagt gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc aaattattac
    29641 atgaattggg cgcacttgtc accgttaatg atggaaaacc ttttgacgaa aatccggaag
    29701 cacaagagct gctttcttta gggataaaag taataacagg tagtcatcca atcgaattgt
    29761 tggatgaaga attttcattg atggtcaaaa atccagggat tccctacagt catcctttag
    29821 tagcaaaagc ccaagaaatg ggtattccgg taattacgga agttgagcta gcttacgaag
    29881 tagcggaatg cccaattatt gggattacag gaacgaacgg aaaaacaacg acaacaacga
    29941 tgacaggact actactgaat gcaggagcag atcaaggaat tgcacgttta gcgggtaata
    30001 ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc agaagataaa attgtgatgg
    30061 aattgtctag cttccaactc atgggaatca cagatttccg ccctcatata gcggtgatta
    30121 ccaatatcta tgaagcacat attgattatc atggtacaag aaaagaatat gtcaaagcta
    30181 aatggaacct tcaaaaaaac atgacagaaa aagattatct gatccttaac tggaatcagt
    30241 cagaactaca agaattagcg caaagaacaa aagcacgcgt actgcctttc tcgacaaaag
    30301 aagtgttgga agacggtgtg tatgctgacg attactcaat atactataaa aaagaaaaaa
    30361 taatggaaat atctgaactt ggcgtaccag gtaagcataa tgtagaaaat gcactagctg
    30421 ctatttctgt agcaaaattg tatggtatat caaatgaagc tatacgagaa acgttgcatt
    30481 ttttccatgg cgtaccacat cgtacgcaat atgtaggaga aatccaaggc agaaaatttt
    30541 ataatgattc aaaagcaacc aatattttag cgactaaaat ggctctaagt ggatttgaaa
    30601 cgtctaaggt cgtgttattg gctggcggat tagacagagg aaatacattt gatgaattaa
    30661 ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga
    30721 tggatgctgg aaaaaaagca ggcatcgaga caatagtaac agccgattct gtagaacaag
    30781 cagttccttt agcattagaa aacagtacag atggtgatgt agttttatta tcaccagcga
    30841 atgccagctg ggatcaatac ccaaattttg aaactagagg aaacagattc atggaagctg
    30901 tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat tttggttaca
    30961 ggcggaggaa cagggggaca tatttatcca gctcttgcct ttgtcaatta tgtaaagact
    31021 caagaaccga atgctgaatt catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc
    31081 gtccctgata caggaatgcc tttccatacc ttggagattc aaggatttaa acgaaaaatt
    31141 tccatgcata acatcaagac ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa
    31201 atcctcagtg attttcaacc agatatcgtt atcggtactg gaggatatgt ttccggagcg
    31261 gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta tccatgaaca aaacagtgta
    31321 ccaggtgtta ccaataaatt tttaagccga tatgtggatc ggatcgctct ttcatttaaa
    31381 gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa
    31441 gaagtagcag atactaaaaa atcagatatt ttgaaaacat ttgatttgga tccagaaaag
    31501 aaaacagtct tgatttttgg cggtagtcaa ggagcactta aaatcaatca agcagtatca
    31561 gtttttttaa gtgaatatat tccttcttct taccaagttt tgtatgcttc aggagataga
    31621 tattatgaag agatcctaaa taaaataaat cctgaaatac aaaatattag tgtccagcct
    31681 tatatcaaaa atatggcaga ggtcatggct tccagtgatc tgctggtggg gcgtgctggt
    31741 gcaacttcga tcgcagaatt cacagctctg gggttaccgg cagtgttggt tcccagccca
    31801 tatgttacga atgatcatca gactaaaaat gcaatgagtc ttgttcacgc aggagcagca
    31861 aaaatgattg cagataatga actaacaggt gaatcactaa gtcagacagt caacgaaatc
    31921 atgggtgatg aagaattgca aaagcaaatg tgccgagctt ctaaagaaca aggaatccct
    31981 gatgcttcaa aacgtcttta tgacttagta aaacaaataa tcaaaaaata aaggcggtgg
    32041 tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca
    32101 atttgactcc ttggcaaaag gctaaccgaa aatatctggc agaacaagag atgaaaaacc
    32161 aagagccaaa aaaagaagac ataacattag atacacccct ttcagattct gaagaagaaa
    32221 aaacggttca agaggaacag caagaagaaa gccagtccga gaaaaaaatc gaattttttg
    32281 aagagatcaa gcaaaagaaa aacggtggac catataacgg ttcttttttg aaccggctgc
    32341 ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg gttagctttg atcatcactg
    32401 tattgggtat accgctgatt tttttgatct attatgtttc tccttatagc aaattacaag
    32461 cagttgcagt atcaggcaat aagacagtca acagccaaga aatcatctct gatacaaaat
    32521 tgtcattagg agaaaatgtt tggggacagt attttcatcg atctacttat atcgatcgcc
    32581 ttaaaaaagc acagccaaga atcgaaacag caaatataca tttcaaaggg atgaatgaat
    32641 tcgatttgga tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac aatcaatact
    32701 acccagtgat tgaaaatgga acagtattag atgaaaaagt agcaaatcct acaaaaaatc
    32761 tccctatctt ggaagagttc aaagactctg caaaaatcaa agaattaaca aagcaataca
    32821 atcagttatc atcagaatta caaaaagcaa tttctgaaat caagtacacg ccaagagcaa
    32881 gcaataaaaa tctgatccaa ttgaatatga atgacggcaa tcaagttatt gtaaatatca
    32941 ataatcttgc gaaccaaatg aaatattatt cgcaagtagc caaagatatg gatgaaaaag
    33001 gtgtgatcga catggaagtt ggtatcttct cgcatcctta cggtagcact caagaaacgg
    33061 aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat caaacaaacg
    33121 aatcaagtga agaggcagaa acgcaaaaag cacaaacacc tggatcgtct agcacaaacg
    33181 gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat agaagataat ccaagtacaa
    33241 ctccgtcaag tttaaatgaa aattgataaa acaactttaa aaagtaagaa tgtactttct
    33301 aaaaaccagt aatttatggt agaattggaa gcagtgaata tatctattaa taagtatatt
    33361 ttaaaacgga cgctagttgt tcgcttgtat atggaatagt aagtaggagg gaatcccatt
    33421 catggcaaaa acaggaatgt atgtaggcct tgatattgga acgacatctg ttaaagttgt
    33481 cgtggctgaa tatatcgaca gccaaatgaa tattattgga gtaggaaacg caaaatcaga
    33541 agggattaac cgaggcatta tcgttgatat cgataaaacg gtccaagcga tacaacgagc
    33601 agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc
    33661 agcaaacatg cttgaagtag aaaattgcca gggaatgatt gcagttaacg gagattcaaa
    33721 ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct gcccttgttc gttcaatacc
    33781 tcctgaacgt caaatcgtgt caatattgcc acaagatttt actgtcgatg ggttcgaggg
    33841 aatcaaagat cctcgtggaa tgatcggcgt aagattagaa atgtacggtc tgttgtttac
    33901 aggacctaaa acgatcatcc ataacatacg taaatgcgtg gaaaatgccg gattaatcgt
    33961 caacgaaatg gtgattactc ctttagcgct gactgaatca attctttctg acggagaaaa
    34021 agattttggt acgatcgtca ttgatatggg tggcggccaa acaacaacgg ctgttatgca
    34081 tgataagcaa ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga
    34141 tatctctatt gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg
    34201 ggatgcatac ccagaacgga catcaccaga tgaagaattt ccagtagatg tgattggtca
    34261 gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa attatttcag ctcgaatgga
    34321 acagattttc aacaaagcaa aagatgcatt ggaccaaatc gaagcattag agttgccagg
    34381 tggcgtcgta ttgacaggcg gagctgctag tcttcctggt gttgtggact tggcacaaga
    34441 aatcttcggc gtaaatgtaa aattgtatgt tccaaatcac atggggctga gaaacccggt
    34501 atttacaaat gtaataagta tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt
    34561 ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt caaatggtag ttgaacaaga
    34621 aacaacaaac acatatgaga gctatgaagc acctgaagaa acaatctacg atgagccgga
    34681 accaaaagag tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta caaatatttt
    34741 tgattaattc atcctgggag gaataagata tggaattctc aattgataat aatatcaatg
    34801 acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa
    34861 tgattgaaga aaacgttaaa ggtgttgaat tcattacagc caacacagac gtgcaagcat
    34921 taaaaaattc aaaagctgaa acagttatcc aattaggccc caaatatact cgtggtttag
    34981 gtgctggttc tcagccagaa gttggacaaa aagcggcaga agaaagtgaa caatccttac
    35041 gcgaagcttt agacggtgca gatatgattt tcatcactgc aggaatgggt ggaggaaccg
    35101 gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca ttgactgttg
    35161 gtgtcgtgac acgtccattc acttttgaag gaccgaaacg tggccgtttt gctgcagaag
    35221 gtatcgctcg tttgaaagaa aatgtggata cattattaat tatctcaaat aaccgtttat
    35281 tagaagttgt agataaaaag acaccgatgc ttgaagcttt ccgtgaagca gataatgttt
    35341 tacgacaagg tgtacaaggt atctctgatt tgatcacagc gccaggttac gttaaccttg
    35401 actttgctga cgtgaaaaca gtgatggaaa accaaggtac agcattgatg gggatcggtg
    35461 tggctagtgg agaagatcgc gtagtagaag caacgaaaaa agcaatctcc tctccattgc
    35521 ttgaaacatc tattgatgga gctgaacaag tacttcttaa catcacaggc ggtttggata
    35581 tgacattgtt cgaagcacaa gatgcttcag atatcgtagc aaatgcagca actggcgatg
    35641 taaatatcat tctaggtaca tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg
    35701 tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga ccagctagac
    35761 aagcacaaat gcaatcacct gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa
    35821 cttcttcaga agaagaaaac agttttggcg attgggatat cagacgtgaa caaaatgttc
    35881 gtcctagagt tgatgattcc aattttgaca atatcgaaaa aaaagaattt gatacattca
    35941 accgcgaaga aacaaaatct catggcgatg atgaattaaa tacgccaccg tttttccgtc
    36001 gtaaaaaata aaggagagga agagcacatg attgctgaca acttgcagaa aatcaagcaa
    36061 gaaattcagg attcgtgtgc tcttgttcat cgtgatccga aagaagttac attagtagct
    36121 gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg ttagagctgg cgttatgaat
    36181 tgtgcagaaa accgagtaga taaattatta gaaaagaaac aagatttatt tgattttcct
    36241 gaaataaagt ggcatttaat tggtaattta cagcgcagaa aggtaaaatt gattgtaaat
    36301 gaaattgatt actttcatgc gttggacagt ttaagattag cagaagaaat ccagaagcgg
    36361 ttagagaaag aattatcctg ttttattgaa gtaaatgttt ctggtgagga atcaaaacat
    36421 ggaatttcac cagatgagct tttaccgttt gtggaatctt tagctgcctg cacaaaaatc
    36481 aaggttgttg ggttgatgac tatggcgcct aaagaagctt ctgatgagca aatccataaa
    36541 gtgtttggta cgctgaaaga attgcgtgat cttgttcaag caaaaggtta tgcccatgca
    36601 ccatgtagtg aattaagtat ggggatgagt caagactttc aaattgcgat tgaagaaggt
    36661 gcaacattta tccgtgtagg aacagcgcta ttcaaagacg aataaaggag gaacaaaaat
    36721 gccgctttta aacagaggag caattgctaa ttttttcgga ttagctgatg aggaagagta
    36781 cgaataccaa gattatcctg aaacaacaaa aaaggctgta cagccaacta aaaaaaatac
    36841 aacacagaat caatcaactc attctgtatc tgctgcgaat actcatgaaa aacaaaaaac
    36901 ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat
    36961 tgaactacat caatcaccta caaaaactgt agcgaaagaa agaacagctt caagaacctc
    37021 tacacaacct aaagcaaatc ttgctggaaa aatcactgta atggaaccaa gagcttattc
    37081 tgaagctatg gcgattgcta aacgaatcat tgctggagaa gcaaccttag tgaattttca
    37141 tttagtagaa gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa cagtttatgc
    37201 actagatgga gatatcaaac gtgttggaga tgagattttt ctttgtacac cgactgggat
    37261 ggagattgac agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga
    37321 ggaggaacta cgatttatac tattgcacta ttacttcatt tacttcaacg ggcagcctat
    37381 ttctatagca ttatcttgat ggtatatgca ttgctctctt ggtttccagg cgggtatcaa
    37441 tcagggctag gacgattatt agctaagatc tgcgaaccgt atatcagttt gttcgatcgt
    37501 ctgcctttga ggattggacc cttggatttc acaattttag gggcaatcct gatcttgaac
    37561 ttagccgtac aaggtttagc acagatcgtt atatggataa ctgtccattt agtttattga
    37621 ttgaagcaac aaaatcttta ttctcagaat agataggagg ggaaaatttt ggatgcaaat
    37681 gtataccagc actttcgggc agatgaacgt acatttatcg attcggtagg cgattggatt
    37741 gaacaggttc aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc
    37801 tatattttag aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga
    37861 tatgaacagg cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag
    37921 gaagattttg aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt
    37981 catgggaaaa tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat
    38041 atcatctcgg acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt
    38101 gctgcacaag taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca
    38161 gaaatgatta tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga
    38221 ctagacagtg tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc
    38281 gaagctggta aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa
    38341 ctattagata ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg
    38401 aagacgaaaa aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag
    38461 gtggtttact catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa
    38521 tgcggggata taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg
    38581 aggaattgac tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac
    38641 ttgagtactt caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata
    38701 cggctgataa agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac
    38761 aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg
    38821 atgcagaaga aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg
    38881 cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga
    38941 tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat
    39001 tctccagcta cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg
    39061 acaatgaaaa tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg
    39121 catttgatac acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga
    39181 acagaaaagc actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca
    39241 ctgcatggtg aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag
    39301 cggctgacct cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg
    39361 tggtaccacg gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa
    39421 ataaaggagt gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat
    39481 tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa
    39541 atcgaatcta ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg
    39601 atggacctcc atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta
    39661 aagacattat cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat
    39721 gggatacaca tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag
    39781 aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata
    39841 agcaacgagc agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta
    39901 cattagatcc ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag
    39961 gctatattta caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag
    40021 cggaagctga aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg
    40081 tcaaagacgg caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac
    40141 catggacatt gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg
    40201 tggaagccga tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag
    40261 aaatcggttg ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata
    40321 tgactgcaag tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta
    40381 ctttagacgc tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata
    40441 tcgctggtag caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta
    40501 cagaagaagc acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg
    40561 atctattaaa agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc
    40621 acgattggcg gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta
    40681 tcgataaatt ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat
    40741 ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc
    40801 agcgtgcatg gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca
    40861 caccagaaac aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt
    40921 ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg
    40981 gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg
    41041 aagctgtttt acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat
    41101 cagaccaata tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg
    41161 tagcaccata caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga
    41221 tgagtaaatc tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag
    41281 atatccttcg cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg
    41341 atatcttaag tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat
    41401 tagcgaatac agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt
    41461 ctgtagacaa atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag
    41521 ggtacgacaa atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg
    41581 atctatcgtc attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg
    41641 attatcaacg tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat
    41701 tattgactcc tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag
    41761 aggagtacgt tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat
    41821 tgatggatac ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag
    41881 aagcaagaaa cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa
    41941 aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg
    42001 tttcaccaga ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg
    42061 tatttgatga tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc
    42121 gccaagttcg taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg
    42181 caaaaatcgt tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa
    42241 agggaaaagg gggtgtgaca tgttctttgt cacaccccct tttgttatca atcgtttaat
    42301 tttccacgtt cacgatacca gttatctggt tgccagatcc attcgaatcc gtctttttca
    42361 agtagttcga aagctgcttt aggtcccatt gatccagctt tgtagttggg aaaatccggt
    42421 tgtgtttggt cccaagcttt tcgaatgaca tccacgatat gccaagattg agcgacttct
    42481 tcccaatgag agaagttcgt tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa
    42541 gcttctgggc tattttcaac catttctgca ctatttctaa attccaattt gattggttcc
    42601 gtttcaaatc cttgccctac ttctttccca ttcaaggata aagaaaatcc ttcagttggc
    42661 tgaatataga tcgttagaac attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca
    42721 gatgttttga atacattaac aggaacttgt ttgaatacga tgttgatccg agtccctttt
    42781 tcagtcaaac gtttgcctgt acgtacatag aaaggaacac ctgaccaacg gaagttatcg
    42841 atagtgaatt tgcctgcgac gaatgtttca gtctctgatt gagcatccac tttgtcttct
    42901 tcgcgatagc ccttgaattg tacaccatcc aattcacctt gagcatattg cccgcgaacg
    42961 aagttttcta acacttcttc ttcgttataa atgcggatag ctttcaatgc tttgatcttt
    43021 tctgtacgga tttctttttc ggaaaatgct actggcggtt ccattgcaag taatgctaca
    43081 acctgtaaaa tatgattttg caccatgtct ttaagtgcgc cactatgatc gtagtagccc
    43141 ccgcgatctt ctacaccgag gttttcgcca aaagtaattt ggatattgtc gatatagcga
    43201 ttgttccatt gagcctcaaa aatattattg gcaaaacgga tagctgagat gttttggatc
    43261 atttcttttc ctaaataatg atcaatcctg aaaatatctt gttctgggaa tacttctctg
    43321 atttgctggt tcaattcaaa agcagactgg taatcagaac cgaacggttt ttctatgatc
    43381 aaacgattga agccattttt tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt
    43441 ccaaaaaatt gaggtgccat agccaaataa tagacacggt taccgtttaa ttgatagtgt
    43501 ccatctaatc ggtcagacaa ttcttttaat gtctgataat gcgttgaatc atttacatca
    43561 tgggattgat agtaaaaatg gcttgcaaag gtagttgctt cttcatctga aggattcaat
    43621 gcacggattg tttctttgac aacttcacgg tagtgctcat cgctccaggg acggcgagcg
    43681 gttccgatta cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac
    43741 gggtacaatt tacgctgggc taggtcacct gtaccgccga atatcgtaaa caatacattt
    43801 ttttcattag acatgtggat acactccttt atattttatc gacaaaaatc gtgctggccg
    43861 ccttaaagct gacggggatt ttctttctat cattttggat agtgatcggt ccttcataag
    43921 ctgcgatgtc aacgatctca tattcttcgt tgatattcag gtcaatagtg actaagtaat
    43981 ccaacaattc tttttcatca agcactcgtg cgatacgcac ttttgtaccg acaggatagg
    44041 actccaatgt ttgtcggcgt tcttcatgga caacttcctc ttttttagga atcgatcctc
    44101 catgaggaca atgttcagga tgattcaaat agttttctaa atgatctgca agatgttccg
    44161 aagtcacatg ttccaatact tcagcatcat catggacttc attccagcta tagtctaaat
    44221 gttcaactaa aaaaacttcc cataagcgat gtttgcgaat cagactgcta gctttttgca
    44281 gtccgcttgc agtcaattgt acaccttgat aaggggaatg ttctacaagc tcttctttca
    44341 caagtttaga aatcatttca ctgacagatg cagctgagac atctagacta gatacgattt
    44401 gtttattgtt gactttcgtt gtatctccac ctagttctaa aatgattttc aaataatctt
    44461 ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt
    44521 aacaaaaaaa acgttaattg actatttttc taaataaaaa acaaaggatt tttagacgtt
    44581 tttacaacaa aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata
    44641 tatatacatt gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt
    44701 cgtcataaag agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta
    44761 cgactaaacg atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca
    44821 aaaacgtgta atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc
    44881 atgtaccttg atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt
    44941 aaataaaact tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca
    45001 ccaaatcact cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat
    45061 tgtaccgaat aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc
    45121 tacaaattat gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa
    45181 gtggaaatag ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa
    45241 gacggagaaa taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata
    45301 ccaggcgctg aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat
    45361 ctgattgctg taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg
    45421 ctggcaattg atggtatttt agacggcacg atccttttac caggattaac tggtccattt
    45481 acggtataca agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg
    45541 gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt
    45601 ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat
    45661 gccgcacaag atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat
    45721 ctggcaacga tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag
    45781 caaagcggag ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc
    45841 aagcaggcca ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa
    45901 ggtaaaataa tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc
    45961 caagcagctt taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa
    46021 aaagcacagg aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag
    46081 acttttttca atattactca tatggacacc atttatattg cttctccgaa tagtcttcac
    46141 tttagtcaag ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct
    46201 ttttcgacgc caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat
    46261 ttttttgaag cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta
    46321 ccattaaaag acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat
    46381 gaccaagtat tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca
    46441 ttgatggatt taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa
    46501 gaagtccatt atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc
    46561 ttacgttatg agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta
    46621 ccgtcagaga tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa
    46681 caagcacaat ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca
    46741 gaaaatccaa tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat
    46801 ccagcttggg gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg
    46861 atttatgatt taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt
    46921 taatgaacga atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag
    46981 ggtttatctc accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa
    47041 gtatagtagc ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac
    47101 tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac
    47161 tagctattca agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc
    47221 aatctttggt gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag
    47281 aaatcttgat tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag
    47341 cacatcaagt gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca
    47401 gccaaatcat tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt
    47461 cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga
    47521 caattgacgt gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg
    47581 taccgatacg aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg
    47641 gtctgatttt ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata
    47701 gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc
    47761 tagaacaatt caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag
    47821 gtttagatat tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag
    47881 cttatttgca tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac
    47941 tcgtacaaga taataatcta agagatttaa aacggctttt acgtccatca aatataacac
    48001 ttgaagagat ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg
    48061 cagctcctgc agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag
    48121 aatataacaa agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata
    48181 aaggagcaag gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat
    48241 tggttgccag aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac
    48301 agaaaaccaa caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag
    48361 caatgcaagc cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg
    48421 ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga
    48481 ctcttctacg caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag
    48541 catacagcag gcaaaagctg cgattcttta tccaccgcgg gggttgcata cgattatttt
    48601 tggagaaact ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga
    48661 ttcagagatg ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgctca
    48721 aaatccgcaa ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc
    48781 agctcaagat agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga
    48841 gatccaccgt ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat
    48901 ttaccgtccg cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc
    48961 gacgactgaa tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat
    49021 caccttgccg aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt
    49081 tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct
    49141 tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact
    49201 agtctgtgca aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg
    49261 aaaagaagaa ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga
    49321 acgtctcgac cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc
    49381 tgatgttgtc tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga
    49441 aaaagtcact tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc
    49501 aaaagatgtg gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc
    49561 caaagatctt ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga
    49621 aaaacggtta gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca
    49681 aagtacattg gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt
    49741 acggaaaaat tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga
    49801 agaagaacaa gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat
    49861 caatctagga gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca
    49921 cggcagacag actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt
    49981 agggaaagct tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt
    50041 acaatatgtc aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat
    50101 gggatcatta aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc
    50161 tatcacgatg acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag
    50221 aagcttagaa gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca
    50281 gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt
    50341 tactggtgaa ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa
    50401 taaggtagaa ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga
    50461 tgccttattg gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca
    50521 aaatatccca tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa
    50581 gcgtatagct gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt
    50641 aagcgatgtc ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat
    50701 ccagaccgat ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt
    50761 agccttcatg ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc
    50821 tattttctca aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact
    50881 agaacgaaaa tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt
    50941 tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct
    51001 gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt
    51061 attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg
    51121 caaaaaaaac aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa
    51181 aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct
    51241 cagatgcaga taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag
    51301 tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg
    51361 tcatcaatat gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa
    51421 atctaatgaa ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa
    51481 tctagaagcc atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga
    51541 agcgattgcg gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc
    51601 agaagaatca cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca
    51661 aggaaatcat atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc
    51721 tattgcgttt actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga
    51781 ataaatacaa cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt
    51841 gttataagga gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga
    51901 ctatcaagtt ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa
    51961 catttagttg ctttgcgtga tgcttttatc ggcacactgc ccgcaacaat ggcgggatca
    52021 gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat
    52081 gctgacagtt tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc
    52141 aatggttttg tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg
    52201 gggtataatc ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta
    52261 gctaccttga ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat
    52321 gttccttcaa atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat
    52381 ggcacactgt cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct
    52441 tattttacag ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt
    52501 gccaacatta cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca
    52561 gccattttac cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt
    52621 cgtgtagctg atgggcaact gatcattgat ttggtgcaaa aatatattgc agagccattc
    52681 ctaggattat ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc
    52741 tggttctttg gtattcatgg accaaatgta ttggcaccag tgctagaggg aatctgggga
    52801 caagcacaac taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca
    52861 ccagccgtgt tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc
    52921 gatgcttttg catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg
    52981 attttctcta aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc
    53041 ttcaatatca acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt
    53101 atcccattct tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt
    53161 ggcctagtcg ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca
    53221 ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg
    53281 actttcttga tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc
    53341 gaacaacaat aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa
    53401 ttttcgagga ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa
    53461 agaattgtta gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg
    53521 aatcaagctg ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa
    53581 ggaactaatc aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta
    53641 gaaaatggtt taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag
    53701 tattggttct ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta
    53761 tagtatggtc acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa
    53821 aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt
    53881 ccagcatcgt ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg
    53941 accggcaaat cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg
    54001 caaaataacc gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt
    54061 cgtacatcga caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat
    54121 tcgacctgtg aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg
    54181 aatgatatgc ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg
    54241 ctagatatga tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa
    54301 aaatgaagta agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca
    54361 ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat
    54421 cttcgattga ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg
    54481 aaaggcgaga acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg
    54541 aggaggatcg gctaacaagt caagtcatgg cgaccccttt tccagtgaat ttttacggac
    54601 aagagtacct aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag
    54661 gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct
    54721 tatcttatct ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat
    54781 ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg
    54841 gaaaaataaa gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac
    54901 agatgccgga aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat
    54961 tcaagcaaaa aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg
    55021 gatatatcgt ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg
    55081 atcatgatgc tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa
    55141 catttgaata ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat
    55201 ttgcttctgt aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt
    55261 tggaaaatta tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc
    55321 attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc
    55381 tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc
    55441 agctattatt agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga
    55501 caaaagaata tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg
    55561 actatttttg ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca
    55621 tttggcattt gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt
    55681 gttttctttt gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt
    55741 ttcccgattt tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc
    55801 attcattgga aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa
    55861 ggactagtgg aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca
    55921 agtaaaaaac aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat
    55981 agagcaatcg cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtacaaat
    56041 aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct
    56101 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
    56161 atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa
    56221 ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag
    56281 attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt
    56341 tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga
    56401 tgacttttta ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa
    56461 agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct
    56521 tagatccttt attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc
    56581 ccaatcgcca tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc
    56641 catcttgtca gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc
    56701 tgctaattct tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc
    56761 tagtgctaag tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga
    56821 ttgagaaata ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc
    56881 atctgtatca agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaacaac
    56941 ttttccatct ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg
    57001 ctttgtactt gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc
    57061 cgtcggcata tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca
    57121 aagatggttt ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgaagggc
    57181 agaaaattgt tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt
    57241 ccatgctaag acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa
    57301 tgcctcaact tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc
    57361 agccgcagcc acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat
    57421 tagtgctttg ccattcgctg attgataaga cgctactcga tggctatcta caccagcaaa
    57481 tgcatcggga tcggcagaaa caacgctgat cctgcttgct cctttttcaa gccattcatc
    57541 cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag
    57601 aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt
    57661 ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac
    57721 aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt
    57781 tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta
    57841 actattagca tgataccatt taattatgat tcaaacaatg aggctttaaa taaaaaaagg
    57901 aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt
    57961 gaagaaaatc aaaaactaat tttgcatatc aaaaaaacat gttacaatga aattaatcaa
    58021 tggtggaagc gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat
    58081 tactagagaa cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg
    58141 atttactgcg agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga
    58201 gtttaaaaat atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca
    58261 atatgaagtg ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta
    58321 tatccatttt gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg
    58381 tggaggaaaa gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga
    58441 tccagcttat gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat
    58501 catagctact gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca
    58561 gattgaaaag atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga
    58621 aatcgattga atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa
    58681 taaggaaaag ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca
    58741 tgaatgcaat gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc
    58801 aagtatatta aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt
    58861 cttcttcagc attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg
    58921 caaaagcagc atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag
    58981 atccgcagac accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt
    59041 ctacgatctc atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat
    59101 ctgcctcctc gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat
    59161 ccagattttc aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc
    59221 tagcatatac gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa
    59281 ttatgaatta taccaaaaca tcattgaaaa gaaaataaaa cctaaagaca cctggggaat
    59341 gtcatttggc taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg
    59401 aaaatatgat tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt
    59461 tattagcaga tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg
    59521 atattgaggt atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg
    59581 gctttgaagg gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat
    59641 tccctcgtaa aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt
    59701 tgaaaggcgg catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga
    59761 tcgaccgatt gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag
    59821 tagggaatcg gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc
    59881 tagcgatcgt tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag
    59941 cagttcctca ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca
    60001 cgatcgaacc tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga
    60061 ctgcgtatac aagagacggc ggactttctt gccaatatga acatacattg gctattacta
    60121 aagatggtcc aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt
    60181 caataggtac gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg
    60241 atgcgcttta ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct
    60301 gtcgtcgttg cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat
    60361 attttaccgt ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt
    60421 cctgagaccg tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca
    60481 ggaggcttgt tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat
    60541 gctttgcaaa ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc
    60601 gtccgtttct tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact
    60661 atcgttcttg ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc
    60721 gtgaacttta tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt
    60781 gtcatcatgt tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc
    60841 attccgggca cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc
    60901 tatgctaggt attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta
    60961 ctcatgcttt ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca
    61021 gttgttcaag aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa
    61081 tggttcaata aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta
    61141 ttgtaaattt atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat
    61201 atgcttgttt cttttagtat ggttataaga ttttttctga caattttatt atcactgata
    61261 ttaacacctg tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa
    61321 agaagaataa ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt
    61381 gttatatcga ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc
    61441 ttgattgctt ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca
    61501 cctaaacaaa aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat
    61561 atacgtattg attccttcac gcttcctttt attggatata tccaactagg ctggcttagt
    61621 tttcccgtaa cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt
    61681 ttggatgggc tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga
    61741 tattttttcc tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca
    61801 agtattatcg gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca
    61861 ggagctctat tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg
    61921 acttttgttt catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt
    61981 tttgctatca ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat
    62041 cttcatcacc gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat
    62101 gcgatggctt tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga
    62161 acgatcgtat tgattattgc catgttgttt gctgcaattt tattatttga attgattggt
    62221 ctgatcaatg agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag
    62281 taccgtacgc aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa
    62341 caaattaaac cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg
    62401 gacactagat aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt
    62461 tgacaacaat tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta
    62521 catcgatatc tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct
    62581 gatagctagc gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga
    62641 gacagttaca gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc
    62701 taagattctt aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt
    62761 tgattacgtt ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact
    62821 ggctgtgttt gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc
    62881 aggtagtttt atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg
    62941 tttcttcatg tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa
    63001 gttattgtac acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa
    63061 tgcaaaactg tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg
    63121 gcgttttttc tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc
    63181 agtcaatctc cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt
    63241 attatttacg ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc
    63301 gtttatcatc atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct
    63361 caagcagatg aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac
    63421 gattattttg aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg
    63481 atgatttttt ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt
    63541 acagctcaag cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca
    63601 ggtgttgcct ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct
    63661 ttagattttt tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag
    63721 atccttcctg tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt
    63781 aatcgatacc gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg
    63841 catttatcca gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc
    63901 aaaaaccggc atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga
    63961 attcgtgcgt tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt
    64021 agatagtatt ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc
    64081 agtagatgat acatgggata tattaactga atacgcagaa aaagataagc gaatccagct
    64141 tttttcgggg cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa
    64201 aggagatcta atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa
    64261 gatcactgac tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat
    64321 tgtggacagc gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga
    64381 aggatggctt aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc
    64441 ttctttaaaa cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat
    64501 tggtttgctg ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag
    64561 acatgattta aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg
    64621 gcgtgttcat ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat
    64681 gtttaaatcc ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga
    64741 aatttgaaat caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag
    64801 cggaacacgt ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga
    64861 caaaccaatg atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct
    64921 gattatttca acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga
    64981 tttaggactg cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt
    65041 tatcattgga gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat
    65101 ttactatggt ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc
    65161 gacagtattt ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga
    65221 gaatatgcaa gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt
    65281 aactggcctt tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc
    65341 agaacgtgga gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact
    65401 atccgtagaa cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt
    65461 gcttgaagca tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg
    65521 tttggaagaa attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc
    65581 acagccattg aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg
    65641 agtccttaca gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg
    65701 acgtctttgg cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag
    65761 aacatggatt gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg
    65821 tccttcgggg gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag
    65881 tcactggagc agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc
    65941 aatgggacgg atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat
    66001 atgcccatgg attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt
    66061 actataatgc agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt
    66121 ggccaattga tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa
    66181 aagaatttga agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt
    66241 ttcttatgaa aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc
    66301 attacgtggt aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg
    66361 caggaaacaa agagaacttg gccggacttc ctagcgatcg cgtagaattg gtcgtaggag
    66421 atgttgcaga tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt
    66481 acgcggcaga atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga
    66541 atatcattgg tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc
    66601 acgtatctac tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg
    66661 gggaaggcga aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt
    66721 cttctacaaa agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac
    66781 gtgcaaccat ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta
    66841 ttcctcgcca agtaacgagt atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa
    66901 agaatgttcg tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga
    66961 cgaaaggcag aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga
    67021 ccgtgatgga attgatctta gaaatgatgg gccaaccaaa agacgcatac gaccatgtaa
    67081 acgaccgcgc agggcacgat ttgcgttatg caatcgattc gacaaaattg cgagaagagc
    67141 taggttggca gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt
    67201 acgaagaaca tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca
    67261 aaaatggaca ataatacaga ggaaatttca ctgtattaat ggaaaaacac atgtgaggca
    67321 ggcgctcgcc tgtctctttt ttaaaataaa aatcaaggag cggataacca tatgttttta
    67381 ttaactggag gaaatggaca attaggtaca gaattgcgcc atttattaga tgaaaaaaat
    67441 ctgccatatg tttcaacaga tgcaaaagac ttggatatca cagatgctga gaaaacaatg
    67501 gcttatatta cagaattaaa acccgatgtt attttccatt gcgcagctta cacagcagta
    67561 gataaagcag aagaagaagc aaaagaatta gatgagaaaa tcaatgtcga tggcacaaga
    67621 aatgtagcac ttgcagcaaa agctgtgaat gcacaattcg tctatatcag tacagactat
    67681 gttttcgatg gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta
    67741 aatgaatatg gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac
    67801 tattatatca tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg
    67861 atgcaacgcc tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga
    67921 ccaacttgga cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca
    67981 tttggtattt atcatttgtc gaacgaaaat agctgttctt ggtatgagtt cgcaaaagaa
    68041 atattgaaag atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa
    68101 gcaacacgtc cgcagtattc agtcatgagc ctgaaaaaga cagaagcgtt aggttttgtc
    68161 ataccaactt ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag
    68221 taatttcctt tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc
    68281 acgacgaaaa gaaaagccaa aagcgaatca ggcagattcg aatttggtgc ttgacctgtt
    68341 tctaaaactc cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt
    68401 aaacggaagt tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa
    68461 aactgatgtg atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac
    68521 acctttttac ctctcacaag ctgaagagga cttttcgttg gtgaacgagg attattcatt
    68581 aaacggacaa aacatgatca ttgcgtataa ttctcctgta aaggatatta cggttatcgg
    68641 aaaaccattc aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg
    68701 ggcgatgaaa ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa
    68761 gaggaaaaaa gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca
    68821 gtggtggttc atgcaacatg tgggcgattt gattttcttt actttgggac tgatgattgc
    68881 tttttatcat tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt
    68941 ggtggttatt ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct
    69001 ggcgtacttg ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc
    69061 atgggatctg ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat
    69121 ggttcatttc tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc
    69181 aggaaaacgg gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac
    69241 aaattggaaa attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt
    69301 ctatcacttt tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga
    69361 tagcgaacaa aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg
    69421 gattaccgtt gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc
    69481 attaagagcc atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc
    69541 ttatatttgg agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa
    69601 tgcatggtct tatttctatg cgattacgca ttcgaaaatg gataattatt tttcggattt
    69661 tgaaaccatc gcagttgctg tgttgggaac agtgatttta gcttttgcat tatttggctg
    69721 gagacgcttg ttttacctcg cttttgtcgc cttagttatc gtatctggct ttttcgtcaa
    69781 tccagtcgta gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa
    69841 aatcgatcaa caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac
    69901 cccaacattg ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg
    69961 gaagaaaatc gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag
    70021 agcttttatc gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt
    70081 cacgttgaat tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc
    70141 attaactgat tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa
    70201 agacggttat atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca
    70261 aactcaggaa aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat
    70321 tagtagaaag gagagtgaag ataattcaag caatcgtaca aaaatttgtc aacaaaatga
    70381 gtgtcggttt cttgcttttg ttgctgacgc tagtcttttt tgtttggaca gcaggggtag
    70441 gttttccagc aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg
    70501 ctattttgct cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg
    70561 ttgcgcttgc ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat
    70621 ttggagactt actgtttcct ttggttattt tattttgttt agtaattaaa tggatcgagt
    70681 ggacaaaatg ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt
    70741 tatatcgtgt atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca
    70801 attcgctttc tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg
    70861 ctttattgat cagtggattt gccagttctt ttgaacgatg gtatgttcga atcatcggtg
    70921 ggatagcagt cgttgcagcc tttttaggta tttgggtctg ccaatctaga ggtgcattag
    70981 tcgcgttgat tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc
    71041 gtgcgccatt tattgcctac acggttacga ttcttttagc tttgccgatt tcttatctgg
    71101 cggctgtctc tgaaaaagtc aatttgttta ccgatcgaga agatatttgg cataaattct
    71161 atcagacgct aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc
    71221 agcgaggaaa ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg
    71281 gattgatagg atttgggatt gctgctttgc tgcttatcct ttttattggt cgcttaacgt
    71341 tgaaagcaga tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga
    71401 tgcagtcatt tatggaggat acattgactt catacccatg ggttccgatt atttatatcc
    71461 tgttggctat ggccgttcat cgatatgatg aaccagaagt tgcggaggaa ttctatgtta
    71521 tggaagaggc atatgacgat atgacttatg atgatacatc ttatgaggaa caaccagata
    71581 gcgctcattc ttccaggatc caacgctatc attgataggc acaaaatacg agagaggctg
    71641 tgacaaccgt cgcagccttt tttggtttat tgcaattgtc tagcatcttc tgtaaaatag
    71701 aaacgttaga gaaaatcttg tatcatagaa aattaagaaa gaaataagaa cgcagctcgg
    71761 taccaaaaat atggtaaaat ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac
    71821 acaaatgttt aaagatttgt atttatttat gaaggacatt tatcagaaca gaaaattatt
    71881 gctacagttt tcgatcaatg acttcaaatc aagatatgca ggatcgtttt taggaatctt
    71941 atgggcgttt atcaatccgc tatttactgt attgatttat tggctggtat ttggtgttgg
    72001 actgaaagca caaatgatcg atgcaaaata cccatttatc gtatatttag ttaccgggat
    72061 gattccctgg ttcttcttct cagatgcatt tgcatcaact acggttgttt ttcgggaata
    72121 tacttattta gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct
    72181 atccaatctg tacacccatt tattcttcat tctgattggc atagctgtcg cgcttataaa
    72241 tggtatatat ccttcagcaa tgtctttaca gctgatttat tatttatttt gcatgtgtgc
    72301 tttcttgaca ggattgactt ggctgacggc atcgatccaa ccatttttac ctgatatcat
    72361 gcaattaatt acgatcgtgc tgcagttgat catgtggaca ttaccgatat tatggagccc
    72421 taatcagttt gattcaggtc ttgtaagcat attgaaatta aatccgctgt actatgttgt
    72481 tcaaggttat cgtgagtcat ttttaagtga agcttggttc tgggagcatg gaacatatac
    72541 tttgtatttc tgggttttca cgttagtaat gttcgtaatc ggctctgcag tgttccgtag
    72601 attgaaacca catttttcag atgtgctgta aggagaatag ctatgacaga atatgcaatt
    72661 agactaaaag atatcacaaa atcttataat atgtacgcaa aaccttcgga tcgatttcgt
    72721 gaagccctca atccttttaa gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg
    72781 aatgtagaaa taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa
    72841 tctacaatgt tgaaaatcat tacgggagta ctgacaccaa catccggcac gatggaaatc
    72901 gaaggaaaaa tctcagcttt gctagaacta ggatcaggat tcaatcctga gtattctggc
    72961 tatgaaaata tctatttaaa tggaatggtt ctaggatttt caagagaaga agtcgatgaa
    73021 atggtcgatg atatcattga atttgctgac atcggagacc atatttatca acctgtaaaa
    73081 acttattcaa gcgggatgtt cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc
    73141 gatattttga ttgtggatga agcattggca gtaggcgatc ttgaatttca actaaaatgt
    73201 atggaaaaat ttacagaaat caagaactcg ggaaagacga ttttattcgt ttctcatgat
    73261 atcagttcga ttcgtcgttt ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc
    73321 gaatatggcg atacgatgga agtaacgacg aattatgaaa actttttaaa gaaaaaatca
    73381 gttaaaacag tggatcggaa taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc
    73441 gacgtgacga aagcggaatt gttggataaa gacaaaaatc cggtggatat gctggaacaa
    73501 ggagaaaaac tatacgttaa agtaacttat gaagtaaaag atgatacaat caaaaatccg
    73561 gttttaggaa ttgcgctacg aacagtcgac aatttttatg tatgcggact taatactcta
    73621 ctagatggta tcaagattcc ttggaaaaaa ggagagaata tcttttatct ggagtatgaa
    73681 aagttaggtt tgttagctgg tgaatattat tttgacgttg cggtctttga agaaaatgca
    73741 acggttcctt tggtatataa gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc
    73801 ggagaaggca tcgtggtctt agaccataaa tgggaagaag ggactcatag caatgaaata
    73861 tgattttgaa atggacctag atgaacaaag cagtgtggga aaaattgccg cacagatcaa
    73921 accaggaagt aaagttttag agtttggtcc cggaaacggc cgattaacga aacatttaat
    73981 cggagcaaaa caatgtgaag tgagcattgt tgagttagat aaggaattgt tcgattttgt
    74041 cagcgaattt gctcaagatg gattttatgg tgatatcgaa agttttgagt gggcaaacta
    74101 ttatgctgga cagacatttg attacgtctt gtttgcggat gttttagaac atttggtaga
    74161 tcctggaaaa acattgaaga aagtcagaga atttttgaat gaagaaggag agatcctgat
    74221 tacattccct aacttggcgc ataattctgt catgatcgat ttgttcaata accaattgcc
    74281 gtgggcttct tatggtctcc tagatgaaac ccataattct ttttatactc atgatggctt
    74341 ccaaaaagtt tttgaaaaag cgggattatt cattaatatt gaagattatt tgtacttagc
    74401 tgtaggtgat acagaattaa aatcaactta tgaagaattg ccggaagctg tacgctatga
    74461 tttcaaaatg cgaccatttg gtgaagtcta ccaatacttt ttctcattga tgaaacatcc
    74521 agtagcacaa agttccattg cagagccaca aaattctaat tatgtaaaag tgttagaagt
    74581 aacccagcaa acgaaacaag atgaaacaat ccaacaaata ccattcaata atttcacagg
    74641 ggaaaatgag acactaagtt tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt
    74701 cgcgcaacaa cctagcttta tcgaattcag cgcagaagct gccggagaaa aactaacatt
    74761 tatcgattcg aatgctgtag tcaaaacagt aaatgattgt tatttgtttg atgggaaaga
    74821 attaccagag ttcgttctaa cggatatttc tgggaaagaa gtgactatcc attgtcacta
    74881 tcgctttatc ggggaattga caccaacaat gaaagaattg ttggaaacga ttcgtcccat
    74941 ggctgaagtc acaaagcagc tttctgagaa aaatgacgaa ttagaaagag aaaatcatca
    75001 tcttcttgag gaaaatacaa gactagacca tacgttaaaa acaaccacaa atcgctattg
    75061 cacattgttg gaaagtgatg aatggacaat aaaacataga ctaggacgac gtaagaaaga
    75121 aacttcgaaa aaaatccaag aaaaagaact ttctatatgt attgacgaga aaatctggga
    75181 tgcagaaaca aaaatattaa aaatcatcgg atggggaatc gctaattcgg atcgccagcc
    75241 cttgtcctat aagttatcag ctgaccaatc gccatttttc gaagcaattc ctgtttcacg
    75301 agaagaagtc aatcaagcag aacagctcgc aaaaggaaca gaagcgggat ttgagttacg
    75361 aatcctttgc gagcaagaac gttcattctt agtggaagca gtagctcaaa atggccaatc
    75421 atggatcatt gaaatgtaaa taatgattaa cttataaaga taaggagtaa acgaatcgtg
    75481 caaaaaaaag aaattgccgt atttatcgat caaatcactc gtgaacgggc cactggcgat
    75541 ttgcttattg tgggctgggc aattgatgaa gtgaccaaag aaatccctac gataaaggta
    75601 gaaaaagaaa atgtgattgc tgaagcaaca catgtcgtaa gattggatat caatcattta
    75661 tataatcttg atgttaagac ccaaagcggt ttcaaggtac gtttatcagg gaaaatgaga
    75721 ggaaaagcca tcttagactt ccaaacagcg agacatcaaa atggcatagc tgtgaaatta
    75781 aacggtaaat acccttatga tgacggcatt gaatcttcat gggaaagaaa aaaaagatta
    75841 ctgaaaaaag ggataaatta tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc
    75901 aaattggaat tgaagccagg atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa
    75961 ccagatttga aagagcaaag gaaagaagca caaggatttg cgtaccgtcc attgatttcc
    76021 atacttatgc ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat tgattcggta
    76081 ctggatcaaa cgtatgatca ttgggaacta tgtatcagtg atgatgcttc tactgatcca
    76141 gctatccgaa agtgtctgga atcctatcaa gcaaaagatg atagaatcaa agtcgttttc
    76201 cgaaaagaaa atggacacat cagtctggca actaattctg cattggaaat agcagaagga
    76261 gaatttatag cactcttaga taatgatgat gaattaccgc catttgcatt atatgaagtg
    76321 gcaaaagtgc taaacgtaca tccagaactg gatgtgatct acagtgacga agataaaatc
    76381 gatgcagatg gcaatcgctt tgatccgcat ttcaaagcag actggtcgcc tgatacattg
    76441 atgggaaata attatatctc tcacttaggc gtatatcgaa caagcattgt taaagaactt
    76501 ggcggtttcc gtaaaggcta tgaaggttct caagattatg atttggtcct tcgtgtgaca
    76561 gaacaaatcc ctgcagatca tatttaccac attgaccgag tcttgtatca ttggcggacg
    76621 atcccaggat caactgcaag taatggcgaa gccaaaagtt atatctatga ttccggagtg
    76681 aaagcattga cagatgccct gtctagaaga aacatcaaag gaagcgtacg tcctggcaga
    76741 atttctggat tttatgagat tgcttatgac gtcttgcaag aggatttggt cagtgtgatc
    76801 attccaacga agaatggcta tgaagatcta aaaacttgtg tggattcgat catcgaaaaa
    76861 acgtcttatt caaattatga aatcattatt gcggataacg gaagtacaga tccaaaaatg
    76921 caagaattat ttgcagaata caagcatcag ctgaaagacc gctttatcgt agaattgatt
    76981 gacattccat tcaattattc tcgaattaac aatttagcag cagaaaaagc aaacggcaaa
    77041 tactttttat tcttgaataa cgatacagaa gtgattgagc cagattggat gacggtgatg
    77101 gtttcttatg cacagtttga tcgaatcggc tgtgtaggag caaaactgtt ttatccagat
    77161 gatacaacgc agcatgcagg agtattatta ggaatcggtg gtgttgcagg gcatgcattg
    77221 aataactatg atcgaacaca ttgtggttat ttcggccgat tggtcattga cgtcaattac
    77281 ttagcagtca ctgcagcttg tatgatggtc aaggcagctg acttcaatgc tgttaacggg
    77341 tttgatgaaa cattagaagt agcattcaac gatgttgact tgtgcttgaa agtttatgaa
    77401 ctgggtcgtt acaatgtcta tgcacatcaa gcagaattgt accatttcga atctaaatca
    77461 agaggttatg aagatactcc agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg
    77521 caagataaat ggccagcata tattgcgcat gatccatttt acaatgataa tttaacaaaa
    77581 gacggaatcg gcgacttttc gttgagacca gattaaacta taataaaaag ctgttatgtt
    77641 gtgattcgct cgcacataac ggctttttat agttttttgt caaaaaaagg cagaaggtgc
    77701 cgactcaaaa aaataagtcg aggttgggac agaagtgttt aactccgaga aataagaaga
    77761 aattcacgaa aattgctttt caaatttttg tgaatttcgg cttatttaag aagaagttgc
    77821 ttctgctccc accgtttatt cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt
    77881 cccacgctct tattcatgct cagagcgcca atagatacaa aatcatagcc aaagtttcac
    77941 gaaccctagc tgtcaaagaa tcgatatttt attttaatat aggctatttt aaaataaatg
    78001 gtttaaggta ttcctctaca aaagccactc tatcaattat catgggttta aaataagaag
    78061 tgatggagat aaccagattg ttcttagcgt caatataaag aacatttcca ctattaccaa
    78121 ttgctgaata tatttcgttc tcagaatcta atacccacca taaatagcca tatctcatat
    78181 tttgtaagtc ttctaaagca ctggttctag gtttgaacat ctcgtttatc cacatgggtg
    78241 gtacgatttg ttttccttta tacatccctt tatttaggca taatacacca atttttgcca
    78301 gatcacttgc cgataaacac aaaccatatc ctgccgtccc gatatttagt ggatcacaaa
    78361 accagacatt ggttttagga gacttatcca ttgtaaactg cttatgttct tcagctgttt
    78421 cagcataata attgacatgg tttggtatcc ctataggatc aaatagatac ttattggcaa
    78481 aatcaacagt tgttagctta cttgtctgat acaacaagcc agttaatatg tgtaaacaga
    78541 cagtttgata gttgaattcg ccggttatac cttttcttcc tcccaataaa tctagtgatg
    78601 tttttgtcca atcttcactt gaacagactt ttgtccatgg atcgccttta catttgtagg
    78661 gtgctttcat tgttagcaag tgcttgattg taatagcgta aatagttttc tccccgcgtt
    78721 tcactttata ctctggaaaa taatctaata ctttatcatc gatacttttg attaatcctt
    78781 gctctaaagc aatgccaatc agcaaagata caatactttt cgttgctgac atgatatgac
    78841 aagtatcgtc ttttttataa ccattccatt catctgtata aacttctacc ccatccttat
    78901 atgcgacaat ttgacatata tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt
    78961 tattcataag atattacctc actttcttct ataagcataa tgtcattaaa aatcgatttc
    79021 aattttttcg attcttcacg taatacaggt attcttatgg actagacagc gctttgggaa
    79081 gatggattct ctgaaaaata taaaggttca ctttatgagt ggataaaaaa agcagcaaat
    79141 acttcgactt ctttaattta ccaacattag tgaaaatcga ttcatctgga gatttgtaat
    79201 ttagagtcct tgtcttgatt agaggtgtat aaagcttttt attagacatt tttaaattcc
    79261 attacaattt gcttataaac taggttttta aaggtgacta tggtaagata atagagatat
    79321 tattttgtga ttaggagtgt atatatgaat aaaaatggag aatggagtgc tgcacgacgg
    79381 atattcatta tattaatgga tgttattgta tacaatgcag caatatatag tagtttctta
    79441 ctgaagtttc gtggagagat cccattacga aatttagaaa ctttccaaca ctcagcaatt
    79501 attatttcag tgatttttat tgcgttgaac attttgttgg gtgcatatgt tttttataat
    79561 cgaatgatca gtgacatcgt gtttgtcaca gtcatcggtc aggtattgat gacactaggg
    79621 atcatggtag tgacttttgc gggccgctgg tttgcttttc cacgtcttgt tatcttactt
    79681 tcatttgttc tcggtacgct tttcttaagt atctatcgta ttctcgtata taaactttat
    79741 ttacgcgtaa gtcgagataa aaaggtcgtc attattgggt tagaaaagga tgtcgcacca
    79801 gcaatccaaa attttggttc aaagaaaaac aacaaacata aagtcgaagc agtcattctt
    79861 gataactatt atgcaaacat caaaaatatg attaaagaca tcgatattgt ctatcttgct
    79921 tctcatatcg aagaaactga aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa
    79981 aaattgtttt tgaatacaac atttgaaaac ttgacattga tcaatccaaa tattatgaat
    80041 ttcgaagatg aaagtattat tgaagcatct ggatttagaa tccaacctga atatgaacta
    80101 ttcaaacgtt tgtttgatat catcgtttca cttgttttat tagttattgc ttcaccattt
    80161 atgttattca cagcaatttt agtcaaagtg acatcaccgg gaccaatcat ctataaacaa
    80221 gtgcggatca caaaaaatca aaaggaattt tcaatctata aattccgctc gatgaccgcc
    80281 acagccgaag caaaatctgg accagtttta gctaagagca atgatgcacg agtaacgcct
    80341 gtaggtaaat ttattcgagc tgtccgcttt gatgaattac cacaaatctt caatgtgctg
    80401 aaaggcgata tgtcgatcgt aggaccacgt cctgaacgtt ctttcttcgt cgatcagttc
    80461 aatgcagaaa acccttatta ctacttacgg cataatgttc gtgctggtat cactgggtat
    80521 gcacaagtct atggaaaata tgtctctgat tacaacagta aattacgttt tgatttactt
    80581 tatatcaaaa aatattcatt gatgcttgat ttaaagatct tgctgcaaac catcaagatc
    80641 ctatttgata aaatgtcctc tcaaggactt gaagaagacg aaaatgtgat ctcattggca
    80701 gaaattgctt ttccgaatga aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat
    80761 ccgaaattca gcataatcgt accagtatac aatgcagaag caactattgg tcgaacgatt
    80821 gatattctaa aagaattgga ttatgaagag tacgaagtcg ttttagtaaa tgatggatca
    80881 acagatcaaa cacaggaaat cattcaagaa ctgattgaaa atgatgaacg ttttgaattg
    80941 attactacga ccaatcaagg gcccggatta gcaagaaacg aaggaataag acagtcaaaa
    81001 ggagaatatc tcttattctt cgatgcagat gacacgccag taaaaacgat cttgtcagat
    81061 tatgatcatt tgctagaaaa gagtccaaaa gcggatcttg ttgttagttc gtttgttttt
    81121 cgtacaatgg acgagggaag aatcgtttcc gaaaaagaaa acttagttac agaatgtcac
    81181 tatctaactc acgaagagtt tataaaagac atgtatcagt taatgaatga acagttgatg
    81241 tatgttgtat ggaacaaatg ctatcgtagc gatattctga aaaataacca aattctattt
    81301 aaaacttata atagttgtga ggatcgaata tttaacttac attattatga atactgtcat
    81361 caagtgatca tgaacccaaa agtagaatat atttatgaat ttgaaggtgg gaaggggatc
    81421 actaatcaat accgacctaa caagttcgaa acatttaaag aattctattc attagcaaat
    81481 actgtaacag acgatattaa taaagacggt atggcggcgc ttcttttaaa agggacaact
    81541 tcagttattt tttctattta tagtacctct gcaagaactt caaaagagaa gaaagacgaa
    81601 gcaaagcaga tattgagtga tccagtgatc cgagaagcca agcaaattgc taagacggat
    81661 tcttcagcaa aaaaaataac aaagttgctt tacaatatgc cagctccact atttttatct
    81721 gctgtaaaag ttggaagctt cgttgaagtg aaaatgccag ggttaatggc tgctttgaag
    81781 cgagtatatt agttaatgga ggaaaatata atgaaaagaa ttttgcattt tcagggacga
    81841 atgggtttag gtggagcaga gtctttcatg atgaatttat atcgtaaaat tgatcgtaca
    81901 aactatcaat ttgatttttt gatttatgaa gattatgcag atgttcaaga ctatcattca
    81961 gagattgaaa gattaggagg ccgtattttt gttgtaccta atcctaagaa aaatattttt
    82021 aaatatttaa tagaagtgaa taagttacta aaaaaagaat ctttttctat agttcacaat
    82081 caggtttatt ttggcggggg aattaattta tggttagcca aaaagaatgg aatcagacag
    82141 agaattgctc atagccatgc aactgaagat ggaaaatctc aaaatattgt aatgaatgta
    82201 ttgcgtaaat ttttaaccaa attgttgtta caaaatgcaa cagactacct tgccgttagt
    82261 caagaagctg gtgaaagtct gtttcaaaac catccctttg agatagttca taatggtatt
    82321 gatttagagc tttatagtaa aaattcagaa gctaaggtta ataagagaaa agaattggat
    82381 atctcaatga gtacttttgt tgtgggaaac attggacgat tagagaaaca aaaaaatcaa
    82441 tcttatttgt tagagatttt tttagaaata ctaaaaatga attctgattc ttacttgatt
    82501 ttagtaggtg aagggtcact tagaaataaa ctgaaagcca aagcagtaga attggatata
    82561 gagaaaagag taaaattttt aggagaaaga aacgatattc ccgagttatt atctgtgatg
    82621 gatgtttttg taatgccttc tctttatgaa ggtctgccaa tggttggatt agaagcacaa
    82681 gcagcaaaat taaaattagt cttagcagat actatttcca aagatacaaa gttaacaaat
    82741 aatgtggatt ttgtatcatt agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa
    82801 gagccgtatg ctaataaaat aacacaagaa ctaaagagtt atgatgtttc gtacactact
    82861 gaacaaatgg agagtatata tagtagagat cattaaaaat gttcaagata ttttaggagt
    82921 gattattttt gaaaatatgt tatttgatat tggcatacaa tgatattggc aacttaaaga
    82981 gattggtcaa tcgattgaac gaaaatgcag tttttattat tcatatagat aaaaaaactg
    83041 atatagaacc atttattgaa gcatttaaat catatgataa tgttgaattt ttatgtaata
    83101 aaaaccgtgt taaaatttat tggggtggtt tttccattgt acaagcagaa atcaatttag
    83161 taaagagagc attacaaaat gagaaatatt taaaatatgt attattatct ggtgctgatt
    83221 atccaatcaa agataatgag tatatttata attattttaa aaagaatagt tcagtggagt
    83281 ttattcgtgg aatagatcta gatcaaatta aacataaaga attttattat aaacatatag
    83341 atgtatatca aaaacatgat tatcctagaa ttaataggaa taacacaact gcatttaaaa
    83401 tttttagagc tataattaat cgttgtctga ggatgattaa acttccccca aaaattagac
    83461 accataaatt tgatttgtat catggttctc aatggtgggc tttgtcgaaa gaatgcctaa
    83521 cagaattaat acaaatgtat gaacaaaatc aacaagacta cctgaatttt aaaataggta
    83581 tgtttgcccc agatgaaaaa ttttttcata cacttttttt taattcctcg ttcaaaaata
    83641 aaaatgttat tggaggacca gatttaccac ttgaattgaa aaatattgaa gaaactactt
    83701 tacaaacttc gaaattagca aatattcata ttattgatcc ttccatgaac aaatggtttg
    83761 atgaaaggga tatagatcaa ataaaagaaa gtgaaaaatt atttgtaaga aaagttaaat
    83821 cggattactc tgataaacta ttaaatagaa ttgataaaga aattttgaaa gttggaaata
    83881 tagatgaata gaatagtacc tgttttagag aagagtttat taagtcgaga tgcgtttcga
    83941 gtaataaatt taatcatttg tctgctttat tttatgccta gtacttttaa tgtaactaat
    84001 atcccgatga aaatagcttt tgcttggggt ggaattatag ttttgtatga ggtttttata
    84061 aagaaaaatt tttttaaaat gcgtttttct tggctactga ttggagcaat gattagtttt
    84121 atcttaacta taatcattaa cagagaaaat tttttaattc catctattta taattttgga
    84181 tatttgctga ttactttgat agtaattttt cctgcagact tttcacaaag tgaagaggaa
    84241 aaaaagaaaa aaatggtgta ttttaataat gttttcatta tcataatttt tttagctgcg
    84301 ctaatatcta tctatcaatt tatttttttg atttcttatc atgtcccaac aggaacgcca
    84361 ggattacttg caagacaagg ttttattgaa catcggttat ttggtgtcta tacaagtcca
    84421 aatgttggag ctatgtttgg atatacttct attattttaa gtttaattag tgtattaatt
    84481 tcaaaaataa caaaaacgat tcgtgttttt tatatgatga attttatcgt acaaatattt
    84541 tatattacac tttcttcgtc tagaggtgcc caggtagtaa tttgttcttt tttagtatta
    84601 tttcttttgt tgtttgccaa ttcaaaattt agaaagagca ttagagaagt actacctgta
    84661 aaaatcaaat ggatggtaag catattatta gttctattgg tatatttttc ttttggtact
    84721 aattatgcta aggatttttt agcaacaatt cctgtatcga ttgatagaaa agtagaagtt
    84781 atagagtcaa atgaatcaga taaaactcca cttccagaaa aagaagtaat attacagcat
    84841 tctagtgata actccgaaat ttcagctggt cgttttacga tttggaaagc tgcgcttaaa
    84901 gttataagac caactttgtt atttggttat ggagagaccg atttctatgg gaatagcgat
    84961 gcaaaatttt tgaaaaaagt agatcttgat actacagatg tgaatgaact aaaaagagca
    85021 catggaaaca tgcataatgg atttcttcaa gtacttgtat caagtggtat aattgctttt
    85081 atatgtatct atgctttcta tgtgttaaat atctttagtt tactcaaaac attttttaga
    85141 agtaaagtga attatgcgtt tcttggtatt gttttaattt ttatactatc tatttttata
    85201 gatgaaatgg tggaggctca tgtacttttc aataagcgcg atgtcatttc aattgttttc
    85261 tggtattatt tgggaagcat ttcttacttg tatttaaaaa aggagaatta atatgaagac
    85321 agctttcatt gcttggactc ctttacattt gattaatata ttaaatgttt atcaaaattt
    85381 ttaccgagaa tcaaaaggag atttatttat ttattctgaa tttgaaaacg ctagaagata
    85441 tagtgagcgt ataaaagaat tagggctatt taatgatgtt gtttttgtga atcaccggaa
    85501 aatcggttct tcctttaaac gtaaaattaa cttgttaact aaccataatc aatttttctc
    85561 ggatgattta tccgtttatg atgaaatttt tatacagggg gaaaattatt tcacaaaaat
    85621 acttttctcc aatgtttaca aaaaaaatcc agatgtaaaa ctaaattata tagaagatgg
    85681 tttgggtact tatt
//
LOCUS       DARDHU010000004        65020 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000004 DARDHU010000000
VERSION     DARDHU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.004"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            315..635
                     /locus_tag="SDD03_000318"
     CDS             315..635
                     /locus_tag="SDD03_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG4302797.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            789..1694
                     /locus_tag="SDD03_000319"
     CDS             789..1694
                     /locus_tag="SDD03_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4302798.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1691..2464
                     /locus_tag="SDD03_000320"
     CDS             1691..2464
                     /locus_tag="SDD03_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG4302799.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2645..4069)
                     /locus_tag="SDD03_000321"
     CDS             complement(2645..4069)
                     /locus_tag="SDD03_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4302800.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4280..4726
                     /gene="nrdI"
                     /locus_tag="SDD03_000322"
     CDS             4280..4726
                     /gene="nrdI"
                     /locus_tag="SDD03_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG4302801.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4791..6773)
                     /locus_tag="SDD03_000323"
     CDS             complement(4791..6773)
                     /locus_tag="SDD03_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4302802.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6787..7683)
                     /gene="murQ"
                     /locus_tag="SDD03_000324"
     CDS             complement(6787..7683)
                     /gene="murQ"
                     /locus_tag="SDD03_000324"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG4302803.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7686..8747)
                     /locus_tag="SDD03_000325"
     CDS             complement(7686..8747)
                     /locus_tag="SDD03_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG4302804.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8958..9524)
                     /locus_tag="SDD03_000326"
     CDS             complement(8958..9524)
                     /locus_tag="SDD03_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4302805.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9552..11054)
                     /gene="eat(A)"
                     /locus_tag="SDD03_000327"
     CDS             complement(9552..11054)
                     /gene="eat(A)"
                     /locus_tag="SDD03_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG4302806.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11312..12433)
                     /locus_tag="SDD03_000328"
     CDS             complement(11312..12433)
                     /locus_tag="SDD03_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4302807.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12559..13500
                     /locus_tag="SDD03_000329"
     CDS             12559..13500
                     /locus_tag="SDD03_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4302808.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13650..14546)
                     /locus_tag="SDD03_000330"
     CDS             complement(13650..14546)
                     /locus_tag="SDD03_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG4302809.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14815..16155
                     /locus_tag="SDD03_000331"
     CDS             14815..16155
                     /locus_tag="SDD03_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4302810.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16373..16816
                     /locus_tag="SDD03_000332"
     CDS             16373..16816
                     /locus_tag="SDD03_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG4302811.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17320..18738)
                     /gene="nhaC"
                     /locus_tag="SDD03_000333"
     CDS             complement(17320..18738)
                     /gene="nhaC"
                     /locus_tag="SDD03_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG4302812.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19141..20364)
                     /locus_tag="SDD03_000334"
     CDS             complement(19141..20364)
                     /locus_tag="SDD03_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG4302813.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20395..21234)
                     /locus_tag="SDD03_000335"
     CDS             complement(20395..21234)
                     /locus_tag="SDD03_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG4302814.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21251..21931)
                     /locus_tag="SDD03_000336"
     CDS             complement(21251..21931)
                     /locus_tag="SDD03_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4302815.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21933..23006)
                     /locus_tag="SDD03_000337"
     CDS             complement(21933..23006)
                     /locus_tag="SDD03_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4302816.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23062..23300)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23343..23576)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23653..24324)
                     /locus_tag="SDD03_000338"
     CDS             complement(23653..24324)
                     /locus_tag="SDD03_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4302817.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24411..27089)
                     /gene="mgtA"
                     /locus_tag="SDD03_000339"
     CDS             complement(24411..27089)
                     /gene="mgtA"
                     /locus_tag="SDD03_000339"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG4302818.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27748..28185
                     /gene="ndk"
                     /locus_tag="SDD03_000340"
     CDS             27748..28185
                     /gene="ndk"
                     /locus_tag="SDD03_000340"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG4302819.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28207..29073)
                     /locus_tag="SDD03_000341"
     CDS             complement(28207..29073)
                     /locus_tag="SDD03_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4302820.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29233..31326)
                     /locus_tag="SDD03_000342"
     CDS             complement(29233..31326)
                     /locus_tag="SDD03_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4302821.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32075..32698)
                     /locus_tag="SDD03_000343"
     CDS             complement(32075..32698)
                     /locus_tag="SDD03_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302822.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32857..34254)
                     /locus_tag="SDD03_000344"
     CDS             complement(32857..34254)
                     /locus_tag="SDD03_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4302823.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34286..35257)
                     /locus_tag="SDD03_000345"
     CDS             complement(34286..35257)
                     /locus_tag="SDD03_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4302824.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35631..36056
                     /locus_tag="SDD03_000346"
     CDS             35631..36056
                     /locus_tag="SDD03_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302825.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36544..37245)
                     /locus_tag="SDD03_000347"
     CDS             complement(36544..37245)
                     /locus_tag="SDD03_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4302826.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37261..37701)
                     /locus_tag="SDD03_000348"
     CDS             complement(37261..37701)
                     /locus_tag="SDD03_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG4302827.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37701..38276)
                     /locus_tag="SDD03_000349"
     CDS             complement(37701..38276)
                     /locus_tag="SDD03_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG4302828.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39124..39603)
                     /locus_tag="SDD03_000350"
                     /pseudo
     CDS             complement(39124..39603)
                     /locus_tag="SDD03_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39762..40463)
                     /locus_tag="SDD03_000351"
     CDS             complement(39762..40463)
                     /locus_tag="SDD03_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4302829.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40571..41980)
                     /locus_tag="SDD03_000352"
     CDS             complement(40571..41980)
                     /locus_tag="SDD03_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG4302830.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(42028..43893)
                     /locus_tag="SDD03_000353"
     CDS             complement(42028..43893)
                     /locus_tag="SDD03_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4302831.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(44014..44865)
                     /locus_tag="SDD03_000354"
     CDS             complement(44014..44865)
                     /locus_tag="SDD03_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4302832.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45175..45888)
                     /locus_tag="SDD03_000355"
     CDS             complement(45175..45888)
                     /locus_tag="SDD03_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG4302833.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45956..46282)
                     /locus_tag="SDD03_000356"
     CDS             complement(45956..46282)
                     /locus_tag="SDD03_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG4302834.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46405..46632)
                     /locus_tag="SDD03_000357"
     CDS             complement(46405..46632)
                     /locus_tag="SDD03_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG4302835.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46954..48207)
                     /locus_tag="SDD03_000358"
     CDS             complement(46954..48207)
                     /locus_tag="SDD03_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4302836.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48268..49008
                     /locus_tag="SDD03_000359"
     CDS             48268..49008
                     /locus_tag="SDD03_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4302837.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49094..49852)
                     /locus_tag="SDD03_000360"
     CDS             complement(49094..49852)
                     /locus_tag="SDD03_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4302838.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49849..50877)
                     /locus_tag="SDD03_000361"
                     /pseudo
     CDS             complement(49849..50877)
                     /locus_tag="SDD03_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50897..54064)
                     /locus_tag="SDD03_000362"
     CDS             complement(50897..54064)
                     /locus_tag="SDD03_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302839.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54288..55172)
                     /locus_tag="SDD03_000363"
     CDS             complement(54288..55172)
                     /locus_tag="SDD03_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG4302840.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55267..55704)
                     /locus_tag="SDD03_000364"
     CDS             complement(55267..55704)
                     /locus_tag="SDD03_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302841.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(55921..56832)
                     /locus_tag="SDD03_000365"
     CDS             complement(55921..56832)
                     /locus_tag="SDD03_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG4302842.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(57289..57717)
                     /locus_tag="SDD03_000366"
     CDS             complement(57289..57717)
                     /locus_tag="SDD03_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302843.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(57745..58383)
                     /locus_tag="SDD03_000367"
     CDS             complement(57745..58383)
                     /locus_tag="SDD03_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4302844.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(58387..60369)
                     /locus_tag="SDD03_000368"
     CDS             complement(58387..60369)
                     /locus_tag="SDD03_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG4302845.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            60549..61214
                     /locus_tag="SDD03_000369"
     CDS             60549..61214
                     /locus_tag="SDD03_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302846.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(63149..>65020)
                     /locus_tag="SDD03_000370"
     CDS             complement(63149..>65020)
                     /locus_tag="SDD03_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4302847.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT        18915 a        13469 c        11016 g        21620 t
ORIGIN      
        1 aatttattgc ccgataatat atagaaataa ggtctagtta ctgttttttt taaatgatta
       61 ataaataaca acttctttct atgaaattgc aaagcattca tcttcttttc cgctaacgat
      121 atatccgtca gtgcttggta cactttacta aaaagtatga gattgccgat atctggatag
      181 gtgtgctata ctacagaaaa agatatacaa tctgccaccg ggtaggataa agaggatgat
      241 cattccatta ggtctttgaa gggatgattt tcctcttttt gtgtattttt ttatagttaa
      301 ggaggaatgt aagtatggca tggatggaat taatcatagc aggaattttg gaagtattct
      361 ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat caattattct ttttacacag
      421 tgattggcat gattgctagt ttttactttt tgtcaaaggc aatcaaaagt ttgccgatga
      481 gtctctctta tcctatctgg acagggattg gcgcagttgg ttcggtcata attggtgtgg
      541 tcttgtttca tgataagctg aatctttcca cttggttctt tgtggggatg ctactcatca
      601 gtatcattgg catcaagatt actagcggcc actagacact tggaataagt actgatcaga
      661 aaaaataagc ctttttattt aatattcaat ttttttatga aaaaataaga aataagtcca
      721 atgacctagc gacttctcca ctcatcaggt gtatgcttgt actatctata gaaaaggagt
      781 gaatattttt gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag
      841 ccctcagcga tgtcagtttt agcttatccc ctggcaaaat cgttggatta gtaggtccta
      901 atggcgctgg aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagatg
      961 gaacaatctg tattggtgaa aatgagattt ctccctctag tcataaaggg ttggaacaag
     1021 taggagcatt gatcgaatac ccgggtatct atccattctt aacaggctat gatcatctga
     1081 agttattctc tgaaaccaac gatgtatctg ctatcgacac tatcgtgaat cagctcaaga
     1141 tggaaaagta cattcacaaa aaagcgaaat cctactcgtt gggaatgaaa caaaaattgg
     1201 gaattgctct tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg
     1261 gtttggatcc acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg
     1321 gcacctcttt cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc
     1381 tgatcctgat caatcaagga aagatcatca aacaatgtac aatggctgaa ttgaaccaaa
     1441 cagcgcaaga tttcatcgtt atctctacaa caaatgatct agctgctcga acgatcttga
     1501 cagaagctgg atatactttg gaagatcctg ataaaatcaa gcttttaaaa gaaaacgaag
     1561 aattattagg aaccgtgatc cagctattgt ctgcacaaaa tatccatgtg acagacgtcc
     1621 agcatctgac gaatgatttg gaatcttctc tattggaaat tctggattca gtaaaggggg
     1681 aagaagaaaa atgacattgc ttgtaaaaca agaattattt aaattgatca agaaaaagtc
     1741 aactgctgtt ttatctgttc tattagttgt tttgctcata ggaacagcac ttctagccaa
     1801 aaaatacacg acgatcattg atccagttga aatgactgct cagcttttta gtgccacatc
     1861 ttggattgtc tttatcatga ttgcagctgc aagcacgatc atttcgatgg aagcacaata
     1921 tggtacattg aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagcaa
     1981 atggatcaca ttagttattt attctgttta tttatacctt ttagctatta ttgtcactgt
     2041 gttgatgaaa ctgattcttt tcccttcgat ttccttcacg gagaaggttt ctactggtca
     2101 aacactgatc caaagcttgt tcacttatac tttgggtagt tatatcggtt tatggctgat
     2161 tttaagttta gtgttgatga ttgcttgctt tatcaatagt tccggcgcat ccatctcagc
     2221 aggaatcgtc ttttattttg cttcgtcaat catatctggt atccttattg cactcatcca
     2281 aaaatgggag tggatcaaat ggaatccgat cagtatgttg aatctgcaaa accaaattgg
     2341 aaacgaagag atcatgaagc aattgactca tctttctact aatcaaatgc tattcggtaa
     2401 tttagcttac atcgttttgt tcttagcatt aggatatctc gtatttaagc gaaaaaatgt
     2461 atgatgactc atcaaaaaag aggctgtgaa agagacgttc agccttattg ccgattggct
     2521 gtctcttaaa caccgtttat tctgaaaaag gagctgtgac aacatttgtg tcacagctcc
     2581 tttcctagta tgtttttgaa taaacgacag gctcacaacc tctctttata cttcttttat
     2641 acttctatac gtgaagcatc ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg
     2701 tcacacctat aagtaaaatc ttatctaagt ggatatgttg tacaggctcg ttttgctcct
     2761 tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag aacaagaaca agcattactt
     2821 cattaaaggc tacgactagc atagcccata cttgaaaggc acttgctaaa agccctacta
     2881 aaaattggac ccattctttc cgctgataag aaagtttcat ttggtacaat cctaagaaaa
     2941 gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc aaagttataa gcactgtcag
     3001 tgaataataa tgaaagtaaa aagacattcg tacataacgt cgtcaatacg atagcataag
     3061 ttggtgcccc atttttgttg agttttcccc atgctttagg caaaagtccg tcttcagcca
     3121 tgtttcccat tgtttcaatc ggtaagatcg tccatgagag ccaaacacca actaaagaaa
     3181 tgatcaaacc gccgttgatc acgactgctc cccatttacc aaccatcatt tcaaacacat
     3241 agcccatagc aggctgcgcc ttgatcgttt gcagctgatt catcgtcatt gtcccatatg
     3301 gaagcatgga aaccaagacg taaataataa tcaagccgaa tatccctaga atcgttgctt
     3361 tcccaacatc tgattgtttt tttgctcgtg aacctacgac agaggctcct tcaataccaa
     3421 caaataccca aagcatcacc atgatgcaac tcaatacttg actggaaaac gggatcggct
     3481 gtccattcgc attcaatata atgtcgctag aaaagttatc aatgaattgt tggatcgaaa
     3541 acgtgctgat agctactaca ataaataaaa ataatggcac taatttacag aatgtgacga
     3601 tagcattgac gaaactagca ttttcgactc cccgattcac tagccatgca tatccccata
     3661 aaaagactgt actcatcacg atcgatgcca gattttgccc accttcaaat actgggaaga
     3721 aatatcctaa tgcgctcatc aacaatgttg caaaagctac gttgcctaac cagcatgtga
     3781 gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac
     3841 taaagatccc gccttttaaa tctggacgtt ttttattgat attattgatc gacaaggaga
     3901 gcatcaacat tgctgttcct acgagtaccc aactgaaaat agctgggccg ccagccgatc
     3961 cggagatgtc cgtcataata ccaaatacgc cacctccaat cgctgaacca attactaagg
     4021 cagcaagtgg aattaaacca attccttttt tctgttgctc tgtatccatg taagcatcct
     4081 ccaaaataaa accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta
     4141 ataaaaactt taatagaaat ataattctac cggctttttt ctcattatac aagcaaagcg
     4201 cctagtccct ttccaaatta caccaccaaa aaacttaaaa aagatgctat actaaaagga
     4261 aagaattgga ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc
     4321 ctttgccaaa catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc
     4381 tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt
     4441 ttttactttc gttcctactt atttagacgg cggaaatggt ttagataacg gtgatacaga
     4501 aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa gacaatcata aactttgtct
     4561 tggtgtagtc ggtagcggaa ataaaaactt caataatcag tattgtctaa cagccaaaca
     4621 atatgcacaa acatttggct ttcctttctt ggcagattat gaattacggg gaacaccatc
     4681 tgatgtgaca cgaatttatc agattttagc agaaaacaat aaataataaa aaagaagaag
     4741 ctgaaaaata gatttcagtt tcttctttcg tttctattgt ttctatcata ttaagctttg
     4801 acatccatca caggtgcttt tgcttttgct ttacctgttt tttccaagac aaagtttttt
     4861 acacgatcca tattggttac caagacgatc atggatgttt ctttgcctgc tgctttgatc
     4921 gcttctaagt cagcttctgc tacaagcgta tcaggtgtca cttgctgccc ttctgttact
     4981 ttcacatcaa atggcgtacc gcctaagtca actgtgttga ttcccatatg aagcaagatt
     5041 tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg ttgggaagac agagctgact
     5101 accccagtaa ctggagaata gatatgtccg ttttctggtt ctacaaagta gccttcaccc
     5161 atcatctttt gtgcaaaaac tggatcactt gctttatcta aagaaacaac tgttccgtca
     5221 gcaactgatg agaggatagt cacactttca gctgaagttt gaggtactac tttttcaggc
     5281 acttcattta ctgtggctgt ttcagtcgct aattcttctt tttctaaacg gtctttagga
     5341 ataccgaaga agaatgtagc aacgaagcca ccagcataag ctgccaataa tccaaggatg
     5401 tagcctaacc atttaccgtc agagatcaat gggatcaaag cggctcctga aggtccaata
     5461 gcgatagccc cgacatttcc gatcattccg atcactgccc cgccgatacc tccaccgata
     5521 caagcagtaa taaatgggcg tcctagaggt aatgttactc cgtaaatcaa tggttcgccg
     5581 attcctagga tcccgactgg taaagctcct ttgatcattt ctgttaattc tttatctttt
     5641 ttgcagcgaa tccataatgc caatgctgca cccacttgtc ctgcaccagc catcgcaaga
     5701 attggaagca atagcgtacg accagtttca tcaatcattt gcacatggat tggagtcaaa
     5761 atttgatgca gtccgaacat aaccatcggc aagaataatg tgcctagaac gaagccggca
     5821 aatgctccac caacgccaag tacccagttg atcccaccga ccatgccatc agaaatgaaa
     5881 cctgctaaag gcatgatgaa gaaaatttca atcagaccga tggcaatcaa tgaaagagtt
     5941 ggtgtaacga tgatatctac cgcatcaggt actactttat gcagtttttt ctcaacgatt
     6001 gacaatagcc aaacagcgaa gatgacacct aggatcccac cttgtccagc tgcaagagct
     6061 tcaccagtaa ataagttttt cagtggtgct tccggattca tccctgtcag catcacaacg
     6121 gccccgataa ctccaccaag agttggtgtt gcaccaaaaa cttgtgcagc attgatacct
     6181 gtatagataa ctaggtagct aaacatcccg tttttcaaga tgttcatcac atcgatatat
     6241 tgttgccatg ttgctgcatc aagtgttccg gctgtgacta agtttgctaa aactgcggca
     6301 atccctgcca cgatcccagt accgacaaat gcaggaatca ttggcacaaa gatattagaa
     6361 atgtctttta ataccgattt gattttagat ggtttttgtt tcgatttttg tgccgctttc
     6421 atttcttgtg cccgttcgtt tacacggtct tttccagatg ctgatgaatt tgctgtagct
     6481 gtacttacag aagaaagttt ttctcctaat ttcacgcctg ccaaatcaac catttgctgt
     6541 gctactttgt tcactttccc aggtccgatg atgacttgaa gctgttcatc ttctactaca
     6601 ccaagcactc ctggaatcgc ttttaattca gagacattta caagatcatc attgatgatc
     6661 ttcatacgca cacgagtcat gcagtggaca atgctttcta cattgcccat gccgcctgcc
     6721 cctttataaa tttgttttgc cagttcttcc aaagtcagtt cgttttttgc catggtcttt
     6781 tccccctcat aaggtccctt ttacaaatcc gtttgcttca gctaattttg cttgagcttc
     6841 ctctttatca ctatttgtca aaatcatgac gatagccagc ttaacatttt gatctgcttc
     6901 ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc gcttgcatga tgattcgttt
     6961 tgagcgttcg attagttttt catttgttgg tttgacatcg accatcaagt tattgaatac
     7021 tttacctata ccaatcatac tgatagtaga aatcatattc aagataagtt tttgtgctgt
     7081 tcctgacttc aatcgagtag agcctgtcaa aaattctgga cctgcatcga cttcgattgg
     7141 gaaatccgcg tgtttactga tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc
     7201 accgatcgat tttgcgtaat ctaatccacc gatcacatat ggtgtacgac cgctagctgc
     7261 gataccaata actatatcat tttttgtcaa attcagcttg attagatctt cttgccctag
     7321 ctcataagaa tcttcggccc cttcgacagc tacagtcatt gcttcttctc caccagcaat
     7381 tagacctatg accatttccg gttctacacc gaatgtcggt acgcattcag cagcatctaa
     7441 cacacctagt cttccacttg ttccggctcc catataaatc agacgtccac cttttttaaa
     7501 ggactcaatc gtgttagcaa tcactggttc gatacttgtc agttcttttt caaccgcagc
     7561 ggccactttc tgatcttctt tgttcattag ttgaacggct tgagaaatac tcatctcatc
     7621 taatccaaaa gtttcttggt ttctgcgttc cgttgttagg ttctctaaat tgattttgct
     7681 catttttatc ttccttcttt tcttataaat tgatagttca taccagcttt gatttggttg
     7741 atcaatggta gctcttcttt gataacttta gctactacat tcactttttc atccgctggc
     7801 aaatcatatt tggttaattg gatctcaccc atatagcgca aatacttttc gttatctaaa
     7861 gtaatagacc ctttcatacg cgtagctgta tttcttgctg ggataggcgg aatctctcga
     7921 aaacgtgcat ctacgcttcg tatgacatct cttgcatcgt cttgccgatt cgtatgcttc
     7981 cctaatatat attcgtaaaa ttcttcatct acaggttccg tatggagcaa gactgtctgt
     8041 tctttttgga aagaagcaaa ttgctcctga acgttttctg acagacctgc atctccgatg
     8101 taaacaatat ccgtgttact tgctaataga tctagagcgc ctgcaagagg atggatccct
     8161 cgatgttctt ccaaagttgg caatccttga tatagtgggc ctctcaactc tgtatctcct
     8221 ggtacaaaac cttggacaag gagaccttga attttgagcc attgattttt tttctgatac
     8281 cattctttgt ccagccctgt ttcgggacgt ggataataat tatgccaagc ttcaatctgt
     8341 gagaaatcag cttctgcttc tttcaattca tcgatatctt taggcgtgat cgtgctagca
     8401 ttcaaactga ttttcaattg atgtgaccag tcggcaatct gctgattgga aatatgataa
     8461 tccattctta aacctgttac accaattgct ttcaaaggcg ttagatcttc taatgaaaaa
     8521 cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca attctagttt attctttttc
     8581 gcttcgcttc ctagttcgat caatcgcttt tgatatgccg aaacatcatc ttctggtata
     8641 tgcatggaag tgaaaatacc gacaaagcca ttggatgcca tttggcggat atactgtttc
     8701 ttctcttctg acaaagaatc gttcataaat attgaaaagc caaacatatt atccctcttt
     8761 ttctgtatcc gtcttcattt ctatgactag agtatatatc taaagaaaca aaatttcaat
     8821 acttttatta aaataattaa atttcatttc taaaggttga gtttatatta tcaaaagaat
     8881 tgtcataacc taaaaaaggc actgctaagc aaaagcagtg ccttgaaata ttttatttaa
     8941 ataacacaaa aacataatta ctcataagaa accaattctg tattatctac catgaatgta
     9001 aagaacccct cattaggcat cggatgaaaa taagcattga tcactcttat caaggcatta
     9061 tggcaaacta acagatagac attttcttcg tcttcgatgc attctttcaa taaaggaacg
     9121 atccgtgcat acacatctaa gactgactcc ccatttggga atcgtacagc gaacgctaaa
     9181 cgtgcttttt gaaagttctc atctttgctt ggcagtccat cataatcccc aaaatccatt
     9241 tctaccaatc gttcatcaac ggtcagcggc aagctgagac gatcagcaac tgcttgtgct
     9301 gtatctcttg ctcgttgaag aggggaatgg atgattttcg tgattggaac aggcaaatct
     9361 actacctttt ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag cggcacatct
     9421 gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct cgttacataa
     9481 agcatgtgat tctctccttc gttggcaaag ttccccttaa ccataccttg ttgttcttga
     9541 gattacaagg gttagattct ctcaagagaa ataatctcag tagatacttt atccaggaag
     9601 gtttgatcat gttccactag taacatggca ggtttcacgt tcaagatcag ttgttctaat
     9661 tgttcttgat tgaagacatc caaataattc aatggttcat cccatgtata tagttcagct
     9721 ggctgtgata aagatttagc caattccact tttttccgtt ggcccatact catctgctcg
     9781 atcttgttat gaaaaacatc tctttccatc ccaagctttc ggaggttgtt caaaaatgct
     9841 tggtagtcta cttggttttt ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa
     9901 ttttggcttg cataactaat gctcagatgt tttggctggt atttttctcc tataaccttg
     9961 ccgttgaatg cccccagaag ataatggata atggatgact ttcctgcacc gttaggacct
    10021 gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa taacagattt
    10081 tcatacccta attgaagatc ttctacgctt aaaagtcgct tatggtgaga cgcttggcta
    10141 ttcatcgtca aagaatcgat atattcgata tcttttaata gtttttcttt ctcgctgatc
    10201 tgggtctcca tccgattcac gattgctttg gatcgtttca tcgtccgtgc agcatcagca
    10261 cccactgctc ctttattcac tcgtctagat tcagtatcga tgaatccgac ttgtttcttt
    10321 gttttatctc cttctcggga acgagaccat tcggcttttt cagctgctgt tttctttagc
    10381 ctactgactt cttttttcaa tttttcattt tgagccattt caaattcatc acgaagtttt
    10441 ttctgttctt catagataga gaaattccct tgataaagtt ccagttgact tttttcgatt
    10501 gctaaaacat ggtccactac ttcatcgata aatccccggt catggctgac cacgatgaag
    10561 ccttgttttt tctttttcaa ataagccgct acttgttttc taccagagat atccaaatga
    10621 ttcgttggtt catcgattaa cgggaaatga gtgtcatcca caaataaaag tgccagtagg
    10681 acttttgtct tctctccccc cgatagtgtg ctgaattctc tccataagat ttcaggatct
    10741 gtttgcatca attggagctc tctttcgatt tcccatatct caaaatccgt aatatcattt
    10801 aacacgtaat aggttaaacg ttctttatct tttgtctgtt gcgggaaata ggcaaattct
    10861 tgctgatgga ttacttgccc ttgataaggt agtttgtttt gtagaatatt cagtaaagtt
    10921 gtctttcctc gaccgtttcg tccgataagt cctagtttcc attgtgtgtc aaaatttaga
    10981 tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt
    11041 tcgattttag acattttcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg
    11101 ctaaagagga tgcaagggtt ggatacacac aaaaagaccc atacatcgaa aaaaccaagc
    11161 caatcccgaa aatctgcaca ctgcttcctt gtctccacaa ggtacagtct gaatgcagct
    11221 ttcatcaatc gacttaattt ttcaatgcac ggatccctca ctttgtccta tatttgaaat
    11281 tacgatagca taaagaaaat gattttgtca actacttcgg gaggatggtg aaaaatctgc
    11341 taagtgtctg tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc attgcttctt
    11401 ctaaacctat tggacccaaa tgaatactta agacaattgg gagaaaatca gaaagtcctt
    11461 ctagctcttg gtctctactt ccgcaaatag cgattattgg ttttcgatat ttttttgcaa
    11521 gcactgctac accgtatgga acttttccgt ggatcgtttg ctgatcgatc cgcccttctc
    11581 ctgtaatgat gacatctgct tgttcgatct ttttttccaa tccagtgatt tggctgacta
    11641 aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag ggctccacca ataccgcctg
    11701 ctgctccact tcctggaata tcatctaaat ccacctccca gaaatctttt atttgctgag
    11761 caatctttcc tgcttgttta tctaagaaag ctagcgtcgt atctattcct cctttttgtc
    11821 ttccaaattg ccaagcagct ccttgttgac ctgtatatgg attggtaaca tctgcagcga
    11881 taatcaattt tactttagaa acaagaacgt ttcttcgatc aaaatgaagt tttttagtag
    11941 ataataaagg atttccttct gtgtaccctt ctaaagaagc acccaaggat tggagtaatc
    12001 ctagaccacc atcgctacac ccgcttcctc ctaaagtgac aataatttgt tctacttctt
    12061 gaagcagtgc atctttaata agttctccta aggcataact ggaagcaagt cggatagttt
    12121 gatccgttgg tttatcaagt agatctaacc ccaatgcttg tgcagattcg atgactgcgg
    12181 tgttttttcc ttgccaagtt gtcaataaat agctgatttt tcgctgacgg aataagagat
    12241 ccatcccatc aatggtacgc catgttcccc tgagcgcgta atagattgct tctgcagtat
    12301 tttctccgcc atctgcaatc ggcacattga tttttcttcc tttacgatct tcccagccat
    12361 ttagaactgc ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg
    12421 ccaaaatatc catctgtatc cctcactttc ttcttaagta agacactttt tttcattttt
    12481 ttcaaacaaa acaatgctat tttcttccat ttttctatat actaaaaggg aactcattga
    12541 ataaaggaga gaaaaaacat gaaaatcgca atagctgggg ccggagcaat gggcagccga
    12601 atcggattga tgctacatca aagcggaaat gaggtcctct tgatcgatcg ttggcctgct
    12661 cacattgaag ccattcgtac aaatgggttg attgctgact ttaacggtaa agaagtggtc
    12721 gcaaaactgc caatctattc accagaagaa atcatcgaaa gcaacgaaca cgtggacttg
    12781 atcgtagcat taacaaaagc caatcaatta gatgatatgt tttgttcgat ccaatccatt
    12841 attactgaca atacttatgt tttgtgttta ctaaatggat taggtcatga agatgtactg
    12901 gaaaaatatg tgccaaagaa aaatatctta tttgggatca cgatgtggac agctggtcta
    12961 gctggtcccg gaaaagtcac attgttaggt gatggagaaa tcgaactaga gaacttggaa
    13021 ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat
    13081 ccgatctata gccataacgt tcgttattcc atttggcgaa aagcttgtgt aaatggaaca
    13141 ttgaatggtt tatgcacgat tttggattgt aatattgctg aactaggcgc gcaaaaagcg
    13201 gcagagagca tggttcgaac aatcgtatct gaatttgctt ctatcgcagc aaaagaagga
    13261 atcatactag atcaagaaga agtgtaccaa catattgctt ctacttatga tcctgacaac
    13321 atcgggttgc attacccatc tatgtaccaa gacctgatca aaaaccatcg tttgactgaa
    13381 atcgactaca tcaatggtgc catttggcga aaaggacaaa aatatgatat tgctacacca
    13441 tattgtgcct ttttgaccca attagtccat gcaaaagaag gaatccttgg cgcagaataa
    13501 aaggctctct gatgagaatc agctgaatca gcagctggtt ctcttttttt aagcccaata
    13561 aaagatcaaa aagaaaacac aactctcatg atgccataag aattgtgctt gtttgtttca
    13621 gtacataatc tcttcaaaat gtaggttttt tatttgtttt caacagagga gaggtcatat
    13681 tcgtattgct cataaatagt ggaatcgtta gtgataaatc gttttgccca attttctgca
    13741 tcataaaatt ccaatagctc tgataggatt ggcttaaatg gtgtatgcag aaattccggt
    13801 aattcatcta aatcacgttc tcgaagcaaa aaaatatttt caggacgata aaaatgataa
    13861 tgagcaatcg cttgattatc cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc
    13921 cgcaaagtca ctccttgctg accctcccgc aacagttcca gaatcccgcg cgattcccct
    13981 gtttttttca ggtactcttc ataagtaaga tatccaggtt gttcattccc tcgatgtgga
    14041 agaatgaaat agttggtcgt aacatctctt tttataaatt cctgcttata gctttctgct
    14101 cgttcttttc tcccattatt cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa
    14161 gaaaaattca tatgtttcga ataaaatgaa ctattatgtt tcagcccaag ttcttttgct
    14221 tccaaaaaat caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc
    14281 atcgcctcgt gttctgcttt gaagtgattc caaaaaaata acacaatttt tgcattggta
    14341 aatgggcgca cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc
    14401 tgataatttg ccaactgttg tctagctgat ttaatgaatt ccgactgtag taaatctttt
    14461 ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat aaagtgattt attttttgca
    14521 actagcagta cgtcattttt gttcatcttt tccgcctcac tttctctgtc actttttcct
    14581 atgatagcct gtttaaatag taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat
    14641 ttaacatttg tatgaaaata tgatgaaata aatcattctt ctattaaaat tcgcctaaaa
    14701 gttttctgaa tagttgtaat tgtctgaaaa tttgttatca ttagtcattg ttaaatattg
    14761 ctatttattc ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa
    14821 gataaaaaat tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga
    14881 gcaatcggtg caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct
    14941 gtattgattg cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga
    15001 aaaatcgttt taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat
    15061 ctaggtgctc attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat
    15121 atcatcgctg aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca
    15181 gcctgggtat ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta
    15241 cggttattcg cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta
    15301 ctgatcattt ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct
    15361 acccttcagc aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc
    15421 agttctttgc ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta
    15481 agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga
    15541 atcatttcct tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact
    15601 ttagcaggca cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt
    15661 gcagcggata tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt
    15721 gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga
    15781 aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt
    15841 gttctttatc ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg
    15901 gcaggttccc tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt
    15961 gtactggcac taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct
    16021 gtcttaggat tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg
    16081 actggcctgc tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta
    16141 ttgacgaatg aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct
    16201 attgaagaaa ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc
    16261 tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc
    16321 catgctcatg acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa
    16381 gtattggaag tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta
    16441 cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt
    16501 gatgatgggt gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa
    16561 caaaaagata aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt
    16621 tatggtacga agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca
    16681 tcattctatc gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa
    16741 attagcggaa gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat
    16801 caaagaaatg gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat
    16861 cagcttcaag aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa
    16921 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt
    16981 ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa
    17041 aatcagcgtt ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg
    17101 ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg
    17161 aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt
    17221 gtataaattt ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg
    17281 attgtgacaa aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt
    17341 cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc
    17401 actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag
    17461 tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta
    17521 gttgattact gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc
    17581 tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc
    17641 aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt
    17701 cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa
    17761 tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc
    17821 tccgcggcta agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc
    17881 gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac
    17941 tgctacagcg atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag
    18001 taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac
    18061 atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat
    18121 gaataaaaag aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg
    18181 tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga
    18241 catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa
    18301 gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc
    18361 actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg
    18421 gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca
    18481 tacagcaatc aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat
    18541 gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa
    18601 catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac
    18661 gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg
    18721 tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag
    18781 ttaagatatc ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa
    18841 agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc
    18901 tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta
    18961 ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg
    19021 tgaaaagttg acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa
    19081 atattttttc atttttctga aaattatttt tataactttc acacacaagt ttatttttta
    19141 ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat
    19201 tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc
    19261 tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt
    19321 gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg
    19381 gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc
    19441 atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac
    19501 gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat
    19561 cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt
    19621 tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc
    19681 tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt
    19741 gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc
    19801 tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc
    19861 tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa
    19921 aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg
    19981 ggtgccatat acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc
    20041 ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa
    20101 agcatcgaat tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc
    20161 atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa
    20221 ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg
    20281 gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg
    20341 gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt
    20401 tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt
    20461 gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc
    20521 ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa
    20581 gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac
    20641 tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc
    20701 tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc
    20761 cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc
    20821 attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga
    20881 ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc
    20941 tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc
    21001 tgctaaagcc acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt
    21061 ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc
    21121 tagttttacg atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc
    21181 caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc
    21241 tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa
    21301 aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat
    21361 accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag
    21421 tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt
    21481 ctaacaaata cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt
    21541 cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa
    21601 caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga
    21661 aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat
    21721 tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg
    21781 tcactactaa cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa
    21841 aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat
    21901 tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata
    21961 cgactatcta aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa
    22021 tccaatgctt ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac
    22081 ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg
    22141 ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact taattccacc
    22201 agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct
    22261 tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg
    22321 aagatctgga tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg
    22381 ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac
    22441 tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca
    22501 ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct
    22561 actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa
    22621 ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa
    22681 tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc
    22741 tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc
    22801 agattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca
    22861 cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt
    22921 tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat
    22981 acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca
    23041 agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca
    23101 agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg
    23161 cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata
    23221 gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact
    23281 taccatttca atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc
    23341 agtaaaagga cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc
    23401 aacatctcaa aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct
    23461 acttatcgac aaattattct ccaaggccat tttcagcttt tcttacttga ctcttctcac
    23521 cagccagagc ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt
    23581 ttgttgttgg gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta
    23641 ttttttctat atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc
    23701 ttttccttcc gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa
    23761 actggaatca ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac
    23821 taaagaatct tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt
    23881 cagataagga atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc
    23941 caagtaatca gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt
    24001 cgtagcaatg cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata
    24061 tggttttaag tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc
    24121 tcggacttcc tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg
    24181 tttccagttt tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg
    24241 ctgaaatccc caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc
    24301 taaatcaaat attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt
    24361 ttagcacgac tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt
    24421 tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg
    24481 actgtagcaa ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca
    24541 ccaaaacttg taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac
    24601 atggcaaatg ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc
    24661 actaaggttt gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa
    24721 atcactcgct gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata
    24781 acaaaataca tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa
    24841 accatgaatt ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta
    24901 tccatccgat cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa
    24961 agttgtaaag gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac
    25021 atattgccaa aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt
    25081 tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg
    25141 tctgcagatt ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact
    25201 ttcattgcag gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta
    25261 cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg
    25321 tcttcagcta cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc
    25381 aattcattga tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc
    25441 ccagtcagaa cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt
    25501 gtttcttttg gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct
    25561 ttgactgaaa attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt
    25621 aagccatctt cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt
    25681 aatggtgtaa cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc
    25741 gcgccttttg tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga
    25801 cgacgttcaa aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct
    25861 aaagtttctg ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga
    25921 tagtagctat ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc
    25981 atatgatatt caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac
    26041 acatcgattg caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg
    26101 gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt
    26161 gttagaccaa ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta
    26221 agcccattga tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg
    26281 gatgttttat ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct
    26341 gctacatgac caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca
    26401 ctgatgacat tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg
    26461 tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg
    26521 gaaatgaata aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct
    26581 gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca
    26641 ttacgtttta cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg
    26701 gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg
    26761 gctacaccga aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa
    26821 ataattccta atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct
    26881 acaatgaatg gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa
    26941 cgttcgattt gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct
    27001 tgtttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc
    27061 tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt
    27121 catcggatcg atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc
    27181 ctttcctcta gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac
    27241 taagtaattt taattcatga cttccaatca tttttcgaat agttattttt ccaattagac
    27301 cacctccttt ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa
    27361 tactcagcga aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag
    27421 ttttgacact gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct
    27481 tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta
    27541 tgtttgtata ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa
    27601 ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca
    27661 cgttatattc ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag
    27721 aaatatagta ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac
    27781 ggcgtgagac gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc
    27841 attgctgaaa tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac
    27901 ttgactgagc gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt
    27961 tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca
    28021 aaagctgccg atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa
    28081 aacatcattc acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc
    28141 ccgccattcg atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact
    28201 gctggtctag ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg
    28261 attctttcct aacatatatt cgagcactgt gaagaattct tggtagtctt tcgtcttttc
    28321 ttcccctaaa gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat
    28381 cactgtactt ctttctttat gtgctaagct ccgtttcata tcttcattca cactacgtgc
    28441 ctttttcaac cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag
    28501 atactgctca tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata
    28561 attgttctca atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc
    28621 aaattgacca tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg
    28681 taaaaattca aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac
    28741 caaataatac agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata
    28801 aggatcaaag ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat
    28861 tggttcatcc agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc
    28921 taaaaatgat ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac
    28981 tagagaagtc ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac
    29041 tcgaagtttc ttgatcaatt caccatcata cataatagtt ctccttttta atttaaattt
    29101 ttccacattc aataaataga tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat
    29161 gtttttttca taaaaaatac caaccttcaa gcattagaat gttgtctaac tactgaaggt
    29221 tggtttgatt gtttattgat tatttttttg ctgttcaacg tctgttcttg ttactggata
    29281 taagttggtt gttgttttat taccattttt tgaaaagaca cttgtcgaat gttcgccttt
    29341 ttgcttttcg attgcttcat tacgttccgc ttcattttta tagtcatatt ttttcggatc
    29401 aatcgtctca aatccgtttg gcacataaaa ccgcaagaga ttttcctgat tcagcttatc
    29461 tgaaagactt aacgcagtct tgactttttc ctgatctttg gagatttcct catcgattcc
    29521 tgctgctttc gtatcgatga tttctcctgt ctgattattg taagcttttc ctcctaggac
    29581 ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca actacttgat catgctgaga
    29641 agataataag tctgtaccaa aatgaagata atctttatca tcgattccca atagatgtag
    29701 taatgtcggc aaaacatcga tttctccacc gtattcatga ttgactgtcc ctttagtata
    29761 accaggcata tggatcatca atggcactcg ttgcatctgt gcgttgtcaa attcatccca
    29821 tgtatctgga tctttaccaa gagcgcttgc taaatcttta ttatctgtat tcgagatccc
    29881 aaaatgatcc ccgtaaatca caaagattga attttggtaa ataccagatt ttttcaaata
    29941 cgtaaaaaat tgttccaatg attgatccag ataatgtgcc gttcgaacat aattatctac
    30001 tgtgctattg ccagtattca aagatggaaa gtcgaaattc ttgtcatccg tataataagg
    30061 cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat gctcaagata
    30121 ttgggcagat tctctgaata aatctttatc taatatccca taacctaacg tttcgtcgga
    30181 ttcatcaaaa taagatcgat caaagaaatt gtcataacct aagtttttat atacatgatc
    30241 tctattccaa aaactagcta cattcccatg gaaaacagcg cttgtatagc cttgcttttg
    30301 tccaaggatc gctggtgcgg cttgaaagac attgtctgac ccaagttctg tgaataatga
    30361 gccttgaggt aaaccaaatg tgccagtttc caacatattt tcagcatcac ttgtctttcc
    30421 ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt gcttgattct ggaaaatact
    30481 atttaaaaac ggtgtgactt cttgtccgtc taccttcata ttaatcaaaa actgctggaa
    30541 actttctaaa tggagaacga tcacattttt ccctttagcg atcccaaatg tttcgggatt
    30601 tggctctgca taatgtttct ttgtataatc taaaacttca tcaatcccat tactgcttgc
    30661 atgcgcgcga acactgctag tcattcctgt tttgattccg tcataaatag tatacgcatc
    30721 gattcctaga tatttgacaa tatatgaacg gtcaaatgtt cgctgcagca attgtggacg
    30781 gttggcttcg ctcagcgcta gatttcctga aaaaataagc cctgaaagac aggtaacagc
    30841 gatagccatc ggtttgccga cagggttgct tttgataggt attttcttga ccttcaacca
    30901 aaccagtagc ccaatgaaca cagcgatatc cagccagtag atgacatcat ggggcttcat
    30961 caaagaaaag gagcttcctg acaatccttg cgacactttc gaaaagccta atacagattt
    31021 gactgtaatg aaatctgtga attcccgata aaaaataata ttcaagtaca acaaaagcgt
    31081 atttaaaata tcaatgatca aaagaatagc taacgctggc tttggctttt taatatacaa
    31141 agaaaggcca aaaaacaata aggtagtcgc aattgggttg atccacaaga taaagtactg
    31201 gatcgtacct tccacaccta atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca
    31261 gaaaaagaaa acagctgtca aaacgattgc taaacgattt tggcacaatt ttttaagttg
    31321 tgtcacgtat atcacttctc tctattcaca ttttttctta ttttgctcct gaaaaatgaa
    31381 aatttcctaa aaaagttgtt atcttaagat ttttttattc tttctaaaga ttattccgtc
    31441 cacttttcac agcataaaac cgctgcgatg taagcgatta aactaatgaa gagataaaag
    31501 tcttgtggtg accgcttcac tccacggtat tcaaaagcca gtttctcggt attcgtacaa
    31561 aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat ttcttgatgc aggacagctt
    31621 tttcacaact tcttccttaa acggtgttct cctgccaact cctcggccac aagtcacaat
    31681 atttataaaa catgagaagc tgttttttga aattgttcct tgtggctcag agttaaacgg
    31741 caggctcaag acctcttgat acacggtgtt caatcagctg accctcggta ttagattatg
    31801 tcaggacgct aattttacaa cctcttggta aacggtgttc aaaagcttgc atctgcggta
    31861 ataagcccaa ccaaacgaaa aatataagga gctatttttt gaaattgctc cttgtggctc
    31921 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccttcgg
    31981 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
    32041 caggtcaaaa cgctgatttc acaacctctg tttttcattg tccataggat tgatattgtc
    32101 cttgtgatga ctcattcgta ataattgagg cttgattttc ttcacttccc gttatcgttt
    32161 gtccactgcc agaatctgaa ggacttgttc ctgtcgactc gtttttttga gaagagacag
    32221 ttgttttctt gacttgttca tttgtctttt tccattccgc taaacgatcg gcgaccactt
    32281 ttttgtctac tttttcagaa taagcggtga tcacttttcc atttttcaaa cgggctactt
    32341 tgtcgccatc atgagtcaaa agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc
    32401 tgagagaaat cgcactaagt acgtcatcga tattaataaa ttgattttct tcttttcctt
    32461 ttggcaaata aatgtccaat tcgccttttt cgtaattggc agttatttta tactccgccc
    32521 accaataagt tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt
    32581 tttctgtttc tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa
    32641 taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc
    32701 tttcctccac tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag
    32761 acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt tttcacaaaa tggtaagaat
    32821 ggagaatttc acgcatttgc tctcaactat tctttcttaa gcctcaaatc cgtttttatc
    32881 tgatacgtct ttgaaccaat gacccgattt tttgattgtt ctttttccat cttgtgctaa
    32941 gtctactgaa ataaagccat atcgattctt atatgcattg gtccacgacc agttatccat
    33001 acatgtccac atatgatagc ctaaacaatt ggcaccttct tgaatggctt gatggacata
    33061 tttcagatga gattggacaa attcgatccg ataatcatcg tggatcatgc catcttcttt
    33121 gataaatcgt gcttcgtttt ctacacccat accgttttca gaaataaagc agtcgatatt
    33181 tccataatga tctcgtacat tgatcaaaat atcatagatt ccttgttcat agatctccca
    33241 tccgcgataa ggattcattt tacgaccagg catttcataa ttgtcgaaat aatcctcagg
    33301 cattggaccg tttttagtat cgattggtgt ttcttttgct tttacccggc gtggctggta
    33361 ataattgata cctaaaagat ctactgtatt ttctttgata accgtcaaat cctctgcatg
    33421 gatcacaggc atgagatcaa gttcttttac tatctcaatc aattcacttg gaaactcgcc
    33481 ttttacagct ggatcaagga atgaacgatt gaagaacgca tctgctattt gcgctgcttt
    33541 aacatcttca gggtttgatt cgtctcttgg atagcttggt gtcagattca agatgatacc
    33601 aataacacca ccttgctcca tttcatggta agatttgatg gctaacgctg aagccaacgc
    33661 ttcatgatag ccaacttgga tcgctttttt catatctgac tcttctggat aatgccatcg
    33721 atataaataa cctgcttcga ctggaacgat tggttcatta tgagtaaacc atttttttac
    33781 tttcgaacca aacaattcaa aacaactttt ggcatatttt tcataggctt caaccgtttc
    33841 tcggttcaac catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt
    33901 cacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac
    33961 ggcttcttcg ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagatgt
    34021 tcgaaatgaa ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata
    34081 aacttgcgag gttttttcag gtcctacttg atcaaagaac ttttgcggtt cttttttgta
    34141 ccaataatca aaaatgttct ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg
    34201 gccacttgct gcagaacccc accaaaaatt tgctggaaat tgatattttt tcatcaaagc
    34261 acctcttttt cttttttagt tcgttttagt tgattccatg tatgatattt tccatcacaa
    34321 tcactttttc attttcttcc tcttcttttt tgattcgttt taacagctgt tctgctgctc
    34381 gttccccgat tttatctgtt ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt
    34441 ttttatctaa ataatcatag tgactgaacg caatatcctt cccgattctg acaccttctt
    34501 gattgaaaca ttcgatggca ccaatcgaca tattgtaatt gctgacaaaa atagcagttg
    34561 cccctttttc taataatgct tttgccccgt aatagccgct ttttctagaa tagtctcctg
    34621 taaagacctc taattggatc gatggatcat tttggaccgc ttctttggct cctgctaaac
    34681 gttctcttgc tacgtagtct gtttgagtgg cagcaataaa tccgattttt ttatgtcctt
    34741 gttcaccatg atgagcgatc acttttgcta cactttctcg atcattcact aatattgtat
    34801 caatacctgc tggtccttct ggacaattca atgcgataac tggaatactc atttttgtga
    34861 gcatttcaaa tccagaccac tcttcttcgg ataagaagac aatcagccca tcgatcacat
    34921 gcttcatcag gtattcaatt ttttgttcaa ttttttctgg ctgatcggca aacccgctta
    34981 ataataagct atacccattt tcttcagcta ctctttcgat ccctgagaca acttccgacc
    35041 caaaacggct ggaaatcccg tttatcaaaa gtccaataga gaagcttcga ttattcttca
    35101 accctttagc aatgatgttt tctttatagt ccaacgctga aatcgcttct gctatttttt
    35161 tcatgttctt ttccttcagt tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa
    35221 caccagccat cttagccaca tcacggatcg atgccataca ttctcactcc tgccagttac
    35281 atttgatttc tctttcttgt tgttcgatta tgatgaccgc attctttgca tagtgttgct
    35341 tgtcatcaat atccatataa ctaaagtctt cagtagagca acttcctctc actattaatt
    35401 atacttaaac aagtatttga accggttcat ttttaatata gcattttttt aaaagattgc
    35461 aacacttttt taaaacgctt tctgaaagtg gcggattttt tgccttttta aaattttatt
    35521 ctaaaattta ctctatttgt gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt
    35581 ttacattcca atggtataat ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat
    35641 taatcgtact atttattttg tctgcgggac ttttgacatt gactggctgt gatttgtttg
    35701 gcggctctga ccaatcctcg gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag
    35761 cagataccac caacagtgta tatacgctaa aagtacgtaa agatatggat ttatccgcgg
    35821 gacagttgac atttacagat aaaaaaatgg aatggacacg cacgtatcag acacaaggaa
    35881 ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac agatatcaag atcactacca
    35941 aagatgacga atatattatt tcaggaaaag aaaataaaaa agaaacaaca gtcatattta
    36001 caaaaatcgg taattaccgt ttgaaggatg aggatggcaa catctacagt ctatagtgcc
    36061 gtctgttgga tcttcttttg tttctcccct tcttttctgc tatagtaaag aagcaagatg
    36121 attgtaaagg agacgaaatg gatgatcgat agtcctgatt attgtacatg taataacaac
    36181 aaaaatatcc atacagaatt taatgaccaa ggaagcttga gcatatgcga caactgtcac
    36241 aaactagtca aagattcatt taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa
    36301 agtagaagaa gataaacaaa tccaagcaaa actcactatg gctgattttt atgaacttca
    36361 aactcagctt cttcctttga tcaagcttca tcagtaaaac aaacagtgga tcatttcagt
    36421 ttacaaatca aagagtaaag atttatttat ttcaaatatt tgaatataaa ataaccaaac
    36481 gatagaaagg cttattctac atcacgtagt aatttttctt tcccgtcgtt tggtttttta
    36541 tttttagcct aattcttgac cttttgcatg ctggttatcc gtaaaatatt ttccagcagg
    36601 ctttccttcc aattcagcta atttttcttg gatctgacgg atcacctttt ttgcatcttc
    36661 tggattttct acaaaatcga atttatcccc atccacttgt attttaggac atacatcaaa
    36721 atcttcatac cattgattat agcgggtgtt caacgtttca taataggtat ataacgtctc
    36781 atcgctttct agctgttcat agtcacgccc acgttttttg atacgttcta gcatcgtgtc
    36841 caaagaaact ttgatatgga ccatcaaatc aggccgtttt tttggagcaa catcatctaa
    36901 ctcgtttagc atcgtatcta ataagctatc gtactgctgt acttcgatat ccgttacccg
    36961 acctaaatca gcgtttagat ggaacaacag gctatcttca tagatggatc ggtctagcac
    37021 attgtcatcg ttcactaaag catttttcat tgctaaaaat cgcttgttca aaaagaagat
    37081 ttgcaataaa aaagcgtact gctcaggatt tgaataaaag agcggcaata cttcgttatc
    37141 ttcgacattt tcataaaatg gtttagaatc aatctctttt gcaagcatat ccgtcaaact
    37201 gctcttacct gcaccaatcg ttcctgaaat caaaatcact gacatacaac ccttcctctt
    37261 ctataagtac tcgcctacgt aagtttcaac aggcaatgtt tcaaaatcat attcacgtac
    37321 agcttggtct gattttaaat acgtatggag actttgtgta atcatcaagt tcgtaggtac
    37381 cgtctcttct tgtacaatga tcatcggttt tttcaacata gttgccacac ctaattcgta
    37441 agccgttcct ggatcgattg tttgatgctc aaagtcaagg atagcaacaa caaattctgc
    37501 atttgtcagt tcttccatat ctttttcgta tacttcctgc gcccatccag cgctgaatag
    37561 ttcataattg ctttcttggt gatgacgtgg agagtagaaa cttgtgactg ttttgttttc
    37621 ctccaacgct ttttcgatac gacttactcg atcgatttgc tcctctgaaa aaaatggtcc
    37681 tgctaaatat atttgactca ttattttgcc tccttcaata ataactgatt tttaatagat
    37741 actgctcgta attcattgac cagttccaat ggattgttgg aaaagattac ttgatccaac
    37801 cattttttat ccataaaatc ctcttttgca aaagtttcta ataattgttg caagccgctg
    37861 taataaccgc caatattcaa tattccgatt ggcttgtcgg gcgtcagacc gattgctatc
    37921 caagaaagaa gttgtgagaa ttcctccaat gtacctagac ctccagggaa aacgaggaca
    37981 gcatctccct tttcaaataa taataccttt ctttcatcga tcgtttctgt aggaataaat
    38041 gtagtaacat cttttccagg caattcagtt tcaaaaagcc cattcggata gatacctgtg
    38101 acttttccgt tattttccag tactgtttta ctaatcactc ccataatccc ggagtatgaa
    38161 ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata
    38221 aatttgtaac tttctttatt accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa
    38281 aaacactccc taactcaaaa gtctatcttc attctacacg tgttttttta aaataaaaga
    38341 ctagattttg tagtttttta gtggtatata ggatagacgc cactatatat agtatttaga
    38401 aaaaaatcat ttttcagcca ttttatagat aaaacaaaaa taaataacgt gttttcatcg
    38461 atatgaaatg acttacttat agaagatact gggatttcgt cctatatgcc aataacgtgt
    38521 attattttaa acaacaaata cttgtttctt ttattaacga aaaaatcatt ttgttctcgt
    38581 gttatgtttc actgataaat gatgtttctc taaataaaaa agccgacgaa ctaaacgatc
    38641 aaaatttgat catttttgtt catcagcttc tcctaaaaat ctttttgtta acgataaata
    38701 gctggatttg taaaaatatc acttgaatcg tcactgctgg aagaaaattc agtgataacc
    38761 gcacctgttg cgcctgcctg gaactagtgt ttgatattgg gctcgaacgt gtactgatct
    38821 ctagcatgaa gaataatttc tttttttact gtataataac tttctttcct ttttggaata
    38881 gtacaggaaa gtttttttgt ttcctttcct tcgacataag gtataccaga ccctttcgac
    38941 aacgaaaggt tctcattttt ccttcatcgt catttcaagg agggtgccga tgctctggac
    39001 agtttcgatc gctcctaaac caaaatctgt atattcgatt ctttctaact ctgattcttt
    39061 cataccattc ctgtctcaag gtacatctat tgtacttttt tcttgtaatc cattctgcct
    39121 ctcctaacta attgtcaaat catcccaaga tatgtcttct tcctcttttt cttcttggat
    39181 gatttctgca ttcggatcta ctggtttttt caataataaa atggttattc ctgtgacaaa
    39241 agaaccagct aacaatgcac caatgaatgc ccatggtctg gtcatcgtag cgactgcaaa
    39301 cattcccgat gccggaatac ggctatctgc gccccaaaac atgcttaatc cgccacctac
    39361 tgtcccaccg actcccgttg ctatgacatt gcgtgccaag tcgttcaata cgatagggaa
    39421 acatccctct gtgatctgac agatccccat aggaaaagcg gtcttcaatg tatcgatttc
    39481 ttgacgattg agtatatttt tacgaatgat tttccctaag aaataagcga tcgtcaagcc
    39541 gaatggggtt accatcgaag ctaaaatcaa tacagtgatt ggttcataga ttccttctga
    39601 aaatcgtgcc acaccccctt atccagaata aatggtaatc cagaaacagc cgtgtatctg
    39661 cggtaataag cctaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat
    39721 tacttgatgc agggcagctt tttcacaacc tcttttttta tttatgtttc ataatcaata
    39781 tggtaaggat gcagctccgt atggtagacc gcctccgaat attcaatcat ttccccaatt
    39841 tgcgtagacg atttccgtat ccgtttgatg ccgatctgtg cgtctgtttt cagaattcct
    39901 tgttcttctt ctgttaaaaa tactgccgag aaatcatcag aaaatgagtc aatagtatat
    39961 ccttttttcg tcagcaaacg atacaaagat tctttgccaa attcttctct gctgaattgc
    40021 atcatttctt gtggaagata atgaacaaaa taaatataag gctgattgtt caaataatat
    40081 aagcgcttaa aacaaacagc ttgctcaccg aaaaaggaaa acaaaggatc ttccttcttt
    40141 atatggacca attgcaaatc cagcgtttct ttccggactt catatcggga gttctccaag
    40201 atttgcgtaa agcttgccgc tttggataat cgattgtaag gacgattgct tagaaccgtc
    40261 gtcccacgac cacttttctt tgcgacatat tcatctgatg ccagcagttc gattgccttt
    40321 cggacagtga ttttgctgac ttgaaacagt tcttctaatt cactttctgt cggcaacatc
    40381 gaaccgactg gatattttcc tgagaaaata tcttctttaa ttttatctgc gacttccaag
    40441 tatagtacac gtgtcctttt cataacgtca ccctttgctt tactattact aaaagaatag
    40501 cacataaaaa ataaaaaaag aacacaaatc acatgtgaga agtgtgttct ttttcattca
    40561 gctgctttaa ttacaagttt acgccatttg aggcaattac ttctttatac caatcaaaag
    40621 attttttctt tgatcgattc agtgtcccat ttccttcatc gtctagatcc acatagataa
    40681 agccgtaacg tttactcatt tctcctgttg atgcactgac aagatcgata catccccaag
    40741 gcgtatagcc catcaaatct acaccgtctg ctactgcatt agccatcgct tcgatatgac
    40801 gacgtaaata atcgatccga taatcatcaa taatcgtgcc atcctcttcc atatgatcaa
    40861 tggctccaag accgttttca acaatgaata atggtttctg gtaacgattg tataattcat
    40921 tcaatgcgat tctcagccct tctggatcga tctgccagcc ccattcactt gcctcaagaa
    40981 acggattttt tactcctccc agcaagttcc cttctgaagt tgctgcttgt ggatcagtct
    41041 cattgattgc cgtagacata taataactaa atccgatgta gtccactgga tattctttta
    41101 gcaactggaa gtctttttcc gtttgctcta atttttccgg atcgactcca tattttttat
    41161 gggtacgcgc tgtgtatgca gggtattctc cacgtacttg gacatccgtg cagaaaaaat
    41221 tgaattcttg gttttggatc attgttgctg cctgattgac tggattggca tcgatgctgt
    41281 aagttgttgc atagataatc atgcagccga tttggatatc cgaacgcaat tcgtggccga
    41341 tttttacggc tttgctactt gcaacgaatt gattatgcca tgcttggaaa atattttggt
    41401 actctcctgc tccattgctt tttactaatc cttgacttaa agctggaaag tgaaaggccg
    41461 aattgatttc attgaaagtc atccaatatt tgactttact gctgtatctc tctaatactg
    41521 tctgagcaaa tcgttcatag aagtcgatca gttttcgatt tttccagcca ccgtattctt
    41581 ttgccaaatg aagaggcatt tcgtaatgag aaatcgtgat cacaggttca atatcatatt
    41641 ttaagcactc atcaatcagc tgatcataaa attctaatcc cgcttcgtta ggagtacctt
    41701 cgtctccatt tggaaaaatt cgtgtccagg caatcgaaaa acgataacat ttgaatccca
    41761 tttccgcaaa caaggcaaga tcttctttga aacgatcgta atgatcgatt cctcgatgat
    41821 tcgggtaaat atatttttct tgatcaatcg tccagtcaaa ttcttcactg ccgataatcg
    41881 caaagcgctg cttacctcca ggcatagcat ctgcaacgga tagtcctttt ccgtctcgat
    41941 cataagcccc ttctaattga tttggcagca gttgctccac cccataaaaa tttttctgaa
    42001 aaacgtgatt gcatcctgat ttcctcctta ttttacaatt gccagaacat cttcgtttgc
    42061 aataatctct ttttggtcta attcaagtat atcggcaaat gcatcagtat tcgtaatgac
    42121 aactggagtg attacgctat agccagctgc tctaattgct tcgatgtcaa aacgaatcaa
    42181 taaatctcct gctttgacga gatccccttg tttgactaag atctcaaagc cattcccatt
    42241 taattctact gtatccatcc cgatatgaat caaaatctcc gctccgtcag tcgttgtgat
    42301 ccctacagca tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag gtgcaactag
    42361 ttctcctact tctggatcaa tagcgatacc ttttcccatc acgccagaag aaaaaacttg
    42421 gtctggtact tcactcaatg gcagtacttt acccgttaaa ggacttttta aggtatgccg
    42481 tgctgaagta atcggttttc ctgcattcgc atgtttgttt tctaattgat tgtcttgtgt
    42541 ttgttcgtca aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag caattcctgt
    42601 agctattact gctactgtca catttgactc tacaccatcc accgtattaa tgaaagtcgg
    42661 aatcgtcaat aagctgacca aactgctgga gaaagctttt actccggaaa atccgataat
    42721 agctccacca atacctccag aaatacaagc ggcaatgaat ggtttcttca aaggtaatgt
    42781 cacaccatac acagttggtt cagtgattcc aaataacgaa gtcactgtag aagaaatccc
    42841 taatgctcga agtttcgtat cttttgttcg caatgctaca gctaaagcag ccccaccttg
    42901 tgccaagatg gctggaagaa gcattggcat caatacatca aacccatatt gttcgatatt
    42961 caaaaacatg atcggtacga atccccaatg catgccgaac atcacgaata cttgccataa
    43021 acttcccatg attagacctg ctacgattgg actaaatcca taaatcgagt tgaagagacc
    43081 gcctaaagca ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg ttaatggaac
    43141 catcactact aaaacgacaa gcggaactaa aatcatttgc agaaattgcg gaatcacttt
    43201 ttttacgaat ggctctaatt tactttgaac ccatacggct agaatgattg gtatgacaga
    43261 agaagtataa ccagatggtg aaagaatcac tgggatccca aagaaactga tagcttctcc
    43321 cgcaccagca actgctgtga tagctggata cacaagtgcc atagcaattg ctactgctaa
    43381 aaactggttg gctttgaact ttttagctgc tgtaaaagca agcatgacag gtaagaaggt
    43441 aaagacacca tctgctgcag caaacaatat ttgataagta ccgctatcag ctgtcagcca
    43501 gcccatcacc gttgccaatg ataagaatcc tttcaatacc ccggcggctg ccatcgcccc
    43561 tagaaaaggt gtaaatatac tagagataat atcgatcaga cgattcaaaa tatttttgga
    43621 ctcttctttt ccatcctccg aaactgcaag accttcctca tccacgatcg attgataaac
    43681 atctgctaca ttactgccaa tcactacttg atactgaccg ccactttgta cgacttggat
    43741 cacgtccggg tcttctttta acttttgcgt atctgctaaa gattcatctt ttaatctgaa
    43801 tcgcaaccgc gtagcacaat gtactaaact tttgacattc ttttgtcctc ctaccgcttc
    43861 ccatacacga cgaccaactg cttgattatc cattgttttt tccttctttc ttttttattt
    43921 aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc tattttttca
    43981 atccaggttt tgcctgccat acagtaacaa tccttatgat tggatgcttt ttttctcact
    44041 tactactctt tgaatatgaa tcgttaaata aatctgctct gcgatcccca tttcctgttt
    44101 ttgcgtttgt cgaagatact cattgatccg ttcgacacaa tgaaacgctt ttggatactt
    44161 tccttgaact aatgcgtaga gaaattcatc ttgctcgtca gatccttccc ctttgagata
    44221 acgctgggca aaaaactgca gatgagtaat gatacgctga taattcaaag attcttcatc
    44281 gaacacgatg ccgaagaatt tactgatgat actcagtata tctcttacca ttgtggtagc
    44341 ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat gaaaggcaat
    44401 aaaaccggct tcatcctcag aaagttccac acccgtcttt tgtttcacga acaaaagagc
    44461 ctctttcgct gcttgatatt ctttcggata aaattttttt gtttcaaata ataatggatt
    44521 aggcagttgg atattttctt ttgctcgtgt gatcgcataa tgaagatgat cggtcaatgt
    44581 cagataaata ttaggcgaaa attcaacttt tagtttatct tcagccagtt cgactacttg
    44641 tttgacccat tcgatctcct ctgccggaat atcagccatc agctgctgga attggttttt
    44701 agcaaaagaa tctcttaaaa caaattcttt ttggaccgct tctggcgaaa tctcgtcccc
    44761 aatctttttt tgaaaagcta gtccttttcc catacaaacg acttcttccc cgaatccatt
    44821 tttcgaaatg acgacattgt tattcaaaat tttatcaatt ttcattttgc ttcgccctct
    44881 caagaaaaat aaaaaaacct agaattcgcc tactgtttct tagctgaatt ctggttttgc
    44941 ctgccgtaca gtaacaatcc atttcttatt caaaatcatc ataccatcga atttttattt
    45001 atgcaagcct tttcatataa aaaataaaaa aactggaaca caagtctatc tagaaaacaa
    45061 ccaaatcaga gatgcacctc tttgcgtttc tctttttttc agcaaattgg tttttcctta
    45121 tcgattcata tcctttggct gtttttaatc tgtaacgact tatgtcccag tatcctaaaa
    45181 tgtttttgtc tctagattga aagcagatct catatcatct gtcgcatctt ttatcgatgt
    45241 aacactttcc agttctcctt gtacaactag tcgcgtgacg gcgtccatgt cgccacaagt
    45301 aattagtgtg acaatttctt ttccctcgac ttcattcagt aattgggtat ctgtaggagc
    45361 aactttttct ttaaaaaatg ttttgtatgt atacacattt tctaaatctg tcaaatagat
    45421 cttggcaccc atctctaaat tttctagcgg tgtaaacagc aattcagggt tcgttgcacg
    45481 atggcttgct aaggcataat ttccttttcc catttcttga tctggagata atgttcctgc
    45541 tccgtaaagt agtgacgtat tatccaagcc tttgaaaacc ggtaaattga ttgaaactga
    45601 agggactgcg atactagcaa tcactggtag atctgttcct ctcatttgcg acctcatgac
    45661 cccttctgtc gtcattggtt ctaccgcatc aaagtcaaaa ctgacatctt tatcgttatt
    45721 ctttttcaga tcttcttttg ttacattcgc tatagcatat ttatctcctg tctctctgac
    45781 aaaataatcc ttgatctgtt cattgaaaat caaagctagt cctactaata acaagaggaa
    45841 caagaggata ttgactagcc atctttttgc ttttcctttt tttttcattt tctctccctc
    45901 cagactcatt ttactgtttt tcttttgctc tttccaacag ccgaatgaca catttctaca
    45961 actctgtgta tttttttcgt acacctgcgc ttttttctac aggatatttc aaattatttt
    46021 tgatcaattt ttcttcaatg atctcatcta gatccaggtg aaggttgtct gccatcatgt
    46081 atgaatagat caatacatct gccagttctt ctttgattcg ttctaattgt gtttctttta
    46141 cttcttccgg cgttttccat tgaaaaagtt ctaacaattc agatgcttcc aaagaaatag
    46201 agatagccaa atctttttcg ttatgaaatt gtcgccaatt acgttcatct ctaaactggt
    46261 tgactttttc catactattc atttgcttgc cttcttccaa tcacttctta cttgattaat
    46321 cataccattt ttttacataa aaaatagatg atgcttttaa aaaaactcct gttttctttt
    46381 atttaaaata aatgtcatag aaaatcaata atttaggtag ccaccatgtg tatgcagtag
    46441 cgtgtcttct tcccttgagg ctttttgttt ttcgatacgt tcttcttccc aaacagacaa
    46501 cgctactaga atctctctta atgattttcc ttcttcggtg agcgagtact ctactttagg
    46561 cgggacttgg ttataggctt ttcgatggat actttcatct tgttctaact ctcttaactg
    46621 ctgggtcagc attttttttg tgatcgtagg gatttgcttg cttctccacg cctaaaataa
    46681 gcgatattgc tcttcccttt atctggtgta agcaaagcag ttagcatttt gacgatcgtg
    46741 ggttccgctt ttcacttctt atctacttag tatcatgaag tatactttcg tactaatact
    46801 aagtagcgga agtgtcaact actttgttta actacatagc aaaagaaaca gaagggtgac
    46861 aacggtcatc acgcttctgt ttcttttgaa tatgctgtgt tgttgtctct atctccgtgt
    46921 agactttcct caaacttatc ctttgacagt aagtcagtag gctgatttgc tctttctaga
    46981 tgccagatac atttctacaa aaaaatagtg attctgtttt gaaaattcga tttttagttc
    47041 tgcattttcg taaacatcca acattttttt cgcactgtat agacctaacc cgccattttt
    47101 tttagaggtt tttcctcttt tgaccatctc cgatagcgtc aaattcgttg gatttgcctt
    47161 gttcgctata ctaaaacgtg atccctcttt ttcttccagc agattgatcg taatcggact
    47221 ctgatcggct acacgatgat cgaatgcgtt agaaataaaa atagataaac agcgaataaa
    47281 aacaaccaga tcaatcggga ttgttttcac ttcgacagga ataactactt ggatcgggat
    47341 tttctccgat tctgcttttt ctagaaattt taaaaagaca cccctgacag ctggggatac
    47401 aatatttgtt agctgttgta tttgcggttt ttcaaaaata gctgcagaat gatcttctaa
    47461 tatttctaaa aattccaacg tttctttttt attttcctga agagtaattt ttaatgaaaa
    47521 cagtagactt ttataatcat gtttgaattc ctctagatga tggtaatatt cttcatttat
    47581 ttgatacagt tgaagcgatt cctgatgttg tctttctttt acttggaaac tgatcaacat
    47641 aatggaaaaa aacaaagaaa aaaaactgat gactatcgac aaaagagata gaagcagata
    47701 attagatgag cggaagtgaa cacttcccaa taccaagtaa atcacgcccc agcttaataa
    47761 aatacaaata cctccaaaat agtgttcagt tggataagta aaaagcaagt tgagaaatct
    47821 cgtctgtttg tctatacttt ttactaatag atagaaaata aatgcgataa gtagtgcaaa
    47881 aaaacttgct gttccaagat gtctctttcc taaataatcc atcagatcaa aaaccagaaa
    47941 aaagctgagt ccagtgatag caaaaaaaag attactgatc aaacagctga taaacaggtt
    48001 tttcgtagta aaataaaaaa gaaacatcaa taataagcca actaggtaag gaaagctcac
    48061 tttagggata ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa agctgttttt
    48121 tactagcagt tgtttcgaaa agaataaata gatacctaaa tctaaaatca aagtaaaggt
    48181 caaataaagt gtcactgttg cgaccatctt attcctccat tgttttgttt caaaatgtac
    48241 acatattttt ttaataggag cctgcatatg aaaaagtatt ttatcataga agataaccag
    48301 tttcacagcg aatggatcat cagagaattg gtcaatcatc atatcgagaa tatcgtgcag
    48361 ctcacttcta tctctcaaat caatcagttg atccagttgg gcactattga taatcacgat
    48421 gttttcttta tcgatatcga cttgaagcaa tcactcacag gtattgatgt cgccgaacaa
    48481 atacgtaaaa aaaatgcaca tagtaatatt gtttttttca ccgcttatac gaattttgca
    48541 gtagagatca tcaatcggaa aatccttccg attggatata tcagaaaaga caggaattta
    48601 tctgaacaaa ttcgtcaagt gctagaacag atcaattgga aagaacagca gatcgtaaat
    48661 gtcgataaaa tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc tcgggaaatt
    48721 ttatatattg aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac aactacagaa
    48781 gataaattag tgaatacttc tttaaaacaa ttgaagacag agttagaaaa gtatgcttac
    48841 tttttacttt taaaatctta ctgtatcaac ttatcgcaat tgcagaaaat tgattctgct
    48901 cattatgtgt tagaattttt caacggtgac tccttactcg ttggtcgaaa gatctttgaa
    48961 aagacaaagg aacgtttcca tgacttccaa aaaactgctt cgagttgatg aataagagct
    49021 gagacgtaag catagcagat ttcagggagt gggccttgaa tcggctccac tccctgaaat
    49081 cgttgatatg attttacgct tcctcttctg taggagcatc tgttaacgac catgtgaatg
    49141 ttgaaacgta gttgtctaca gcataattgg atacaggaac aaataattcc actgattgtc
    49201 tcccttgttc cacattctct cctaatgaat aaatccatgt gccaatccct tcatttgctc
    49261 ctgcagttac tagatctact gcctgatctg ctgggatttc gattccttca gcgccagttc
    49321 cagaaacggt agatggcatt ccactgctat tagttgcgct atttcgaatc acggcgtttt
    49381 tgaatttcac ctttgaacca gttagtttat tcgtatcaga tgttgtacca ataaattctg
    49441 ttcgtttgac tgataatttc caaccggcat aatttccgct tttatccgtt acttgtacga
    49501 aattctgtac ttgtgtttgt gctccagtac tgtccgttcc gctttgtaat gcagcatata
    49561 ctgtttttgc tgtacctaac ttgattgttc caaaatcaag atctggagct tggtcaatcg
    49621 tcagtaatcc ggatgttccc tgatttggtt cgtcaggtgt aaaaggagta tcaggatcat
    49681 ttggatcaac aggaggaact ggaacaacgt cttcaggatt tgcagcatct ggtttggtca
    49741 cagaaatcgt tccttctgta gtggctgagc tcttattctc agcaaaaaca ctttgtgtac
    49801 ataataatag acttccagct agaacggtac ttaatgcaat tgtttttttc atttcttttt
    49861 ccttctttct tttaaaataa ataatccagc aaataagcca ataagaatga ctccaacaag
    49921 tatcagccat cgccagtcaa atgtttctgg ctgatttttt aaatcaatat ccttttcatt
    49981 caattggtcc gctgcatcat cagttatttc aaaatcggta tggagattcc agcgtttttc
    50041 tttccaagca cccttcacaa ccgcttgata atgtcccgga gctaattttt ttccttctaa
    50101 tgggacaagt aaatcaaaat ttgtattggg tgctacatct attttttctt tttttattga
    50161 aaggtaaggc tcttttgttt cttttttgta gatttcaacc gatgtagtca agccgaacat
    50221 ataagaagca tttggatttt gaagattcgc cttaatcaca tttcttccat cgctttgatc
    50281 cgcataagct ttttttaatt tcaaatctgg ggaagactct ttttcgctat ttcaaatcaa
    50341 gattgctttc acttgagaaa actgattgac gacaccaact gtacttttgt tagaaacgtt
    50401 ctcttttttt tcttgaaacg tgatccctcc tgcgaccaca ccagccagct ctttttctgg
    50461 cgctttcagt gaaagtgtct cagtgaccgc actttgagga ggaacagtta tttctcccac
    50521 tgtttttgct aagtttttca tatcataact taatgtttca tcaaatttac tttttgattc
    50581 tccatattcg atcacaccgt ttgaattcgt cgtagcggag tggatcaatg tctcaactgt
    50641 tactgattta tctgttcggt taaagaccgt gtaggggatt tcctgtgttt tcccaggctc
    50701 catccttaca tttatatatg ttttgttctt atcaatctga ttagccggta attcaaaatc
    50761 taccgcaaaa ttgaattcgg aggcatggac aggtgtctgc caaaggatga atccaaggag
    50821 aaggatcata agtaaagagc agttgtttcg atgactttta ttccatattc gattcatcaa
    50881 tcatcctcct tgatctttat tctgtcggcg catctgctaa tgtccatgtt atctctgctg
    50941 agtaattacc agctatctga ttttctttgg ttaagacaag tttaaacgcc cgaccgttat
    51001 ttaaatattc tgaataatct gcttgcacat tcgttgaatt ttgtcctctg acaatttcca
    51061 gttcttcttc attcactaat tgatactcct ctcctttctt gaaatatagg acatcctgca
    51121 tagaaaatcc tgcatcattt gtgaaaggct gagataattt tatcgttaag atccacggat
    51181 ttttcgtttc tcgctgatca taaatccata atttttcctg tatatccatt tggccaaatg
    51241 cttcttgtgt caaaccagtt gctttgatct gaccaaaatc gagtttggaa ggggcaagaa
    51301 atgagagacc accttctact acgatcttcg tagcaggtgc aaaaggcggg gcatcatgat
    51361 aaacaactgg cgtatcactt ggattgtgat tcccaatttc atcgttggta cttggcttgt
    51421 tctgcacacc cttagcagta gcttctcctg cttggtcgtt taatacaatt tctaatacat
    51481 ctcctgcaaa aagttgattt tctgggatag aaagaacaaa ttcccctgta tcatcaatag
    51541 ttgtttctga caaaacagct tgattttttt tcaatgaaac caaggctcct gcttcacctg
    51601 tacctgtgat cgtgcgagaa gctgcattga tcaatgtggt acttaaatta gcaggttcag
    51661 gaggaatcac atcgactgtc gttttggcag agtccagttc tgtcacttct tggccacctc
    51721 ttactttttt agcagcaaca acttgcactt gatcctccgt ttttaatctc tcatcattgg
    51781 gtacttgaac ttcgaacaag ccgccttgtt cgccttctcc ccatgggtta tatcctgccc
    51841 cttcattttc atctgtttgt ccttgcgtga gagcagtaat ttctacagat gatttcgtcg
    51901 tatctgcgta agtaatacgt aaagtcactt ctacttcgcc tgtccaagca tctcttggct
    51961 ttgcctcgac accttcggga acaacgacat gcccatagat ttttttatct gcattcgttg
    52021 gcactcttaa ggattcaatc atcgggagct gattattggc actgattcga ttataatttt
    52081 tcattccggc acctgtctga gcaaattcat ttgttaaacc tgcactaggg cttgatacga
    52141 aattgcctaa gtcggtccca ttgatcgata agtctgtcat atcagaaaaa taatccgggt
    52201 ctttttcaaa gttatcgaat gttgcgcctg tttttcgcca taaagcgatt tctgaatggg
    52261 cgatctttaa agaagaatct ccatttcctt ggaataccca gccgcctatg ccattggtct
    52321 cgcttccaat ccgtttgttt tggaaatcaa aataggtagg agattctaag tccacattca
    52381 acacatcccc actgatgatg ccggtcttat cttcccctgt tttcccgacc atgactaaat
    52441 aagttttttc acctgccgta aaatttaaat caatatctcc agttgtatct acagttgctc
    52501 cggaatctgc gcgtatatcg atatttgatt tttctccagt gactgtaaaa tcagctgaac
    52561 ctggactttc tagaaaacca gaagcgtcat aaaattggat cgcttgattt ccttgattga
    52621 gtctgttcac gtaagatttg ccatcacctg tatttcttac cctcaatgag cctccattgg
    52681 ttactgtcag cttttgcgta ccattctcaa aacgaactgc gctggaacgc ccactctgct
    52741 tatcgatgtc gataactgat ccggctcctt ctacgatcaa agaaaggcct tttcctacaa
    52801 aacgcatccc attgttcccg ggttgatcgt catctccagc cattttgacc gagatttgtc
    52861 caccacttga tacatggatt tgactagaat ctccttgcaa gcgaaatccg cccgtagttg
    52921 ctgtattgat ggtgattttc ccaccattcg atacagtgac tttcgatcct gaacctctca
    52981 tttggatgat tccgcgattg ttgtcattgg taatattgat ctttgtattt accgaaaatt
    53041 cgcttcccga accactgact tcaaacatga cattaccagg tagcgtatcg actgcctctc
    53101 cttcatcgct aacgccatct gcccgatcat aatcaagaga gacctttgaa ccgcttaaga
    53161 tatggattcc tggcgctgtt tttccactac cagtctcatc tcctgaatct agaaaaccgt
    53221 agaatttccc tagatacggt ccataaagtt gactaccatt gatcacattc aattgatagc
    53281 ttctacaaaa ataggcttta cccggagcgg cattcagtcc ttccttcact ggttggacat
    53341 ccaacacacc ggaaacacca tctaatgtga cagttgcacg aggagcgtaa atcagtccta
    53401 atttggaccc atcatacaaa tcaagtgtcc cggtaaattt tatttctccc gtaaaatgat
    53461 atctgcttcc ccaaaaatcg gtcatataag caatgataat ggcatttcca tcgatatttg
    53521 gtgtcaatcc acctggatgt gcagctgttc ctttaaaatt ctggaactcg atcgactgag
    53581 gtatctcatt gatataaata tggtgtgtgc cgaaattgtg agcgaatcca ccaaaatcaa
    53641 taacgatatc tcgtttgatc tctaattccc tgtctaaagt caaaggctga atcagtcgga
    53701 tcttagttac tgattcgtct gcgagaagct gcgccagttc ttctgttgtg gcagcttcgc
    53761 tgtacccatc cgctgaaaca gctcttgcca gtgccgcttc actgctatca gacgtttgtt
    53821 cagctacttc tgaggtctca cttgtcttat tttcttcttg ccttttatta gtagaagcct
    53881 cactttgaga ttctgtagtt tcagaagtaa tcgattcttc tgaaaaagta gtcttgtcaa
    53941 aactttgatt ctctgattgc gttgattcgt ttgttttctc tacatcggca aaaacggatg
    54001 tcaccatcaa gagagggaag acaaacaaaa tcgacgcaat cagctttttt acacattgat
    54061 tcattctcca ccatcctctc tgacgttttg acaattttat tttatcgttt taatctattt
    54121 ccaattgtac tttcatattt aaaatatgta tttttatgga aaaatttttc tattaggtgt
    54181 aaatttcttt tttgtacaaa aaaaaggctg tgacaaaagt cttgtcacag tcccttatcc
    54241 cgaacaagcg gtgttctccc gccaactcct cggtcacaac ctccatttta acaataaacc
    54301 tgtaaatgtg agggcaatac tttgattgtc acaggtaatt tatccccttc atctccatca
    54361 acattcgtcg tcaattcttc ctcatcagct aatgacacaa ctaattgctc acaagtaagg
    54421 taggctagat ttttcgtaga ttcatctact ccttttaata ggtctggcaa tgttttaagc
    54481 gtatcgatca aagaagaatc ttttaaataa accaaatgga gcttaccatc atccactttt
    54541 gcatttggaa gtaatgtttc aaaaccgccg actgagttag ttagtcctat cagcaaggta
    54601 ctgctctcaa tttcttcctt ctttccatca acttctacgt gaaacgagta ggattgtgtg
    54661 gaagctaact gtttgattcc tgacatgaaa taagctagtt ttccaaattt cgtcttcttc
    54721 tccgcatcta catcattgat tgattcaggg atagagccaa ttgcgacgac attcataaaa
    54781 tagtcgtcat ttatttttcc aatatccaat ggcttgactg attcaatcga aaaatgattg
    54841 atcgcttcct caggttctag cggtatccct aatgctcgag ccaagtcatt caccgtaccc
    54901 aatgggaaaa atccgaaatt tggccgatgt tcttgttcag cgattccact gattccttcg
    54961 ttgactgttc catctccgcc catgacaaac acactatgat acccttccgt agccgcttcg
    55021 cgagtaaagt tttttgcatc ccctgctttt tttgtatgca agacgaccac ttcatcaaac
    55081 accgattcca attttgctat cgctaattgc tcaaattcct ttgcttgttc accgcctgaa
    55141 ctggggttta ccactaataa aacttttttc atcactttct ttcccttctg attgatttct
    55201 ttaatataac gatagcataa agaaaaaaat taggctgtga tgaagtactg ttcatgaaac
    55261 attcatttat tttttgacaa gataaaacat ccatactatg aaactgagga aaattaaaac
    55321 aatgatatag gaaataaaag aaaatgatag gcattgtata cctaataaag taaaaatctt
    55381 aggtggcaga taaaaacaca aaaatagcaa gagaatgtaa tagcgtttct tccacttttt
    55441 gttatcagac cttatagcga tcagaggaat accaaatgca taactgaaaa actcttgatg
    55501 tactgttaat atgtcacggc cttgaataat aaatgcgaca atccccgcta taattagtgt
    55561 gcctaacacc aaaacggaaa attgatgaat atattttata tacaaatgat ccattgtact
    55621 tcctgccttt ttatttgtga gaaaaacaaa tccgtatacg tgaataactt ctagaaataa
    55681 aagaaatttg caaaaccact ccatttatct acttctacgt gcattttttg atattgtttt
    55741 ctttatttaa gattatacag aaaaatatct agggtcgctg gattctaagt aaaaaaaatt
    55801 tttaaaataa aaagaactaa atcaagtagt aattaaacta ctgttgattt agttctaata
    55861 aaaagattta gatgaacagt tttattgatt taaatagttc atatttttta tttataccgc
    55921 ttatttacta cttgctgtac tagaatctga agtgaatgta ctcaaaacat tttcaaagtc
    55981 tttgtctttg atttttacat tagcgtcttt caatacttcg ccaattactt tattctggaa
    56041 ggtactgtca cttaattttg tgtctgttgc aatttctttc aattcttttt tgtatttatc
    56101 catgtcgttg cctttgtttt ggtttttcac cattttaacc acataatatt ccgtagtata
    56161 agttgaagca ttggtagaag tgatcacatc agaaacttga ccatctttca acttaaaggc
    56221 tgcttctttg acttctgccg gaaccgttgt agtagtggaa tcaaatttca cttttccgcc
    56281 atcttctttt gtcgtagtat cagttgactt gtctttggct aatttagaga aatcgtctcc
    56341 cttatcagca gctttcttca catcttttgc atcatcttca cttgctactt ggataatttg
    56401 agcttccact tctggatgga aagattccca agccgttttt aaatcttcat cggtcaattt
    56461 gatatgtttc tttagacctt cttgaaaggc tagactttgt ttgatactgt ctttgaatgt
    56521 ttgttctgtg taacctgcag atttcaattg tgaatcaaat gaatcaccca attgtttctt
    56581 tgtttgatca tattgagaat cgatttcttt ggttgatacg tcatctccat atttatcttc
    56641 aaatacttga taaacgatca actgttgcac agcggaatct gctccttgca tttttttcaa
    56701 gctatccatt acttcttgtt cgctgattgt accgccttta taactaacgg ctgttttttc
    56761 accggcattc gtacagcctg acaataagcc agtgcctagt acgatggcaa gtgctaaaaa
    56821 tctttttttc atttatcaat tctcctactt ttcgtttttt gcgaattgct aaaaacaaag
    56881 gtgttcaaga tgaaaatcat caagaacacc tttactaatc tgctcgatac gtcctgaata
    56941 gatataaggc tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc
    57001 acaccttaat atactacttc ttttgttacc gtttttcaag tctatcttgc gttccgtatt
    57061 taacgtaact aattcttaga acaaatgtta tcgattttgt ttttcttctt ccgctgctgg
    57121 gatattcaaa tcagacactt ttatgttcga ttttccagac atttttttgt tgtttttatt
    57181 atgatatatt tttatacaac taaaaatgaa tatattaaaa ctaccaaaaa taacttttaa
    57241 cgctgtttca ttatatgttt tttctatttt atataaactt ttggatactt aataccaacc
    57301 ggttggattg taccaacatg agaatgtgtc agttctattt cctgtcgcac ttgcataggc
    57361 ccatactcct tttgttgcat ttgctgatcg aatagagttt ccaactttcg cttttgcacc
    57421 gtgacgatta tagacacgac tatagtggtt agaatgacct gtttttctcg tacaagttgt
    57481 atcactataa taaacccatc ttccagagct atgattaatt gctgttctag aaatttcccg
    57541 gtctactggt aaatttgact taataatatt acctttagca tcaaaatgaa tctctcctgg
    57601 ctttaaagat ttgtaataga tttcaccgtg aactgaattt gcttccactt cagatgacat
    57661 agcgtttcct ccaacaaaac ctatccctag tgcacaaata ataactgctt ttttcataaa
    57721 acgacatcct ttcctttttg atttttaaag catgatcttc ttgtttccta ttttggtcag
    57781 atatggatca tgcgtgacta cgataactaa cgtaccttgg gaagcttgtt gcgataagat
    57841 ttcaactact ttatccttat ttcttttatc caatgaccca gtcggttcat cggctaaaat
    57901 gattttgcta ttcttcaata agactctcgc acaggcgatt ctttgcttct caccaccaga
    57961 taatgtgtag ataggcgtac ttagcttctt gtttatattt acttttttca atgcctcata
    58021 ctttgctgtt cgcttatctg aaacatttaa attcgaaaac ttcaatccaa tatctaaatt
    58081 aaaatcgatt gtttcatatt caatcaaccc aaaatcttga aaaagaaaag atatggtatt
    58141 tctttttatc aagcgcttct ctctcccagt taattgtttc gtctcttgat cgaacaaata
    58201 gaattgtcct gaactgaact gatcattcaa tcctaatata ttcaataacg tacttttacc
    58261 tgctccactt tctccaacaa cagtcacaag atccccagca tccgcagtga aacgatccaa
    58321 atgaagaatc gttttgtttc gaatgatttt tttcgcacct tttatcacta tcaacggctc
    58381 caattgttaa cctccattca tcagcaataa ctgacttttc ttttctgtag acaaaataaa
    58441 aacaactgaa agaataaaat caatgaagct aaagaaaaaa atattaaaaa gatatttagg
    58501 ctctctttct ataagaaaaa ggaagattac acttgtcgtt ccccatttga tgaggttcat
    58561 catgaagaaa tctttatacg tttcaaagag cgaataacca cttgaacgaa agatagcgat
    58621 tttccgacaa ttgtagacaa aatatatgca taacatgaca tagctcaaca agaagaaaga
    58681 aaagaaagtg agtaaaaata tctgtatcaa gtaatgaaat gatctgctta acactttgac
    58741 agctatctct tcagtctgag tatacggaaa tgataactgc aagttatcac gtaactgact
    58801 ttcttcaatc agctcaaata acctttcttt cgacaaagag cctatcttga tctttaacgg
    58861 tggatagatt tctccgctga gaatatttct ttccaaatac atactgtttt tcaatgtcag
    58921 tatttcaaca accggataat gttcgatcca ggggatagaa ggaataaatg tataaatggg
    58981 ttgattgttt tttataaata gaaatttcgg ttctttttca ctgatttctt gataatattc
    59041 tgttatttgc gccaggtcat tttttaaatc tgtatgtcta tatcgttcag ggatcaatat
    59101 aacagggtgc gtttcttttt cggttatttg gatcaacctt ccagagacat caatcaaagg
    59161 aaatttctta agataattca gatttatttt tatccctctg ccaaactgtt cattttcttc
    59221 attcaagtaa tctgatacat tgactaatat acttccgttt ttattcaaag tttcatataa
    59281 ttcctgatct gctgctgatg caaaacgatt ggaataaata aaatccacgt ggtttttccc
    59341 gatggttact ggataaaata catgatagtc ctcaccagcg atttcttttg gtggttcatg
    59401 ggaaacagct aactgtgaag aagaaaaaac gatagttgct aattcgacat tggcaacggt
    59461 caaaagaaga gaaaacaaca atccaaatgt aaaaagaatg tattggatat agttgaacgc
    59521 ttctcttaac gaagcgattt tttcaactaa taaaagagaa atcaaagaga taccatatat
    59581 tccgctgaac aagaaaacgt acatctggaa caaataaaga aattgctcct tataatacat
    59641 aaaaataaaa ccaccaatga ttcctgcaat tatgaatgta ataatgattt tctttttccc
    59701 caataaacac cacaatactt gatgtaatga aaagtcatta ttcaacaata aactgatttc
    59761 tttattcatc aataagaaaa aggatgataa caaaaggaat aaaagaatca atccaatagt
    59821 taaatacact cgataattat atacgtctgt atttgaaata accgaatacg aatagctatt
    59881 ttcaaagtct tcaaaaatat tagtggcata atcagtgtga aatgcttgat tgaatcgatc
    59941 agtgaatagt gaaatgaacc gttcataggc ttctttttct ctcgtcatca agaaaaattc
    60001 accttcaaaa tcttttattt tgatcgattc ttcaatggac gttacgcgaa aggaggtatt
    60061 agaatagggt accttctcgt gagaagattt gacatcccct tgataataca atgtcatttc
    60121 aagctttggc tgtatcttga agttccaaga tgtatctact tcattggatt catttactgt
    60181 ttttttcata taagaaagct cagtttctga tgagacattt tcaaatacca tgattagctg
    60241 ctcatattct tcttgcccaa taaaatgttg tggcaatcga atcggagtat aagtatctgt
    60301 ttgatggaaa aattcatttg tccgatagag tacctgttta tttatactaa ccaaggcaaa
    60361 accactcatg ataccggtca ctatgattgt gagtaaaact gtgaactttt ttttgattat
    60421 caccactctc ccttacttaa atgattcttt gataaaatca taccgttttt catcaaaaaa
    60481 acgtatctat caagtgtata ggtcatgtag aataaatcta tatactgtag aatcgaaagg
    60541 agaagaagat gaacaccgga gaaaaaatac gaacttaccg cagaaattgt aagcttacgc
    60601 aaaaagaatt aggcgaaaaa attggtgttt ccgataaaac gatttctagt tgggaaaatt
    60661 ctcgtacaat gccagacctt gaaatgctgt cacttttaca tcatacttta ggtctgccga
    60721 ttgattttcc ttctattgaa tcagctaccg aatcaataga accaacattg ccagatgaac
    60781 ctcttgttaa caagaaatct tattcatcaa aacaaccctt ctcactcaaa aagacactat
    60841 ctttctttct gctcgtcttt tgtttggtct atctttttct tatcaattat caatacaaaa
    60901 atgaaacgat cgatcgattc aatccttttt tgaaaccaca aatcggctat accacactcc
    60961 ctgtaggata cgtggacaat tccatcgaac gttgggtatg taatgatatc tttggccatg
    61021 gtacatggct gtcttttcac gctggagagg tagaaaaaga gaaaaaccat gctgtaatca
    61081 aacacaaagg aaaacacgtc atgaacgttc gcctgctcac ttgggaagcc attccctcag
    61141 attaccaaga aatcctcagc caagactatg acgacgcctt aaaaagaagc cctcaggagc
    61201 cagataggca ttaagtagga gatgaacgta gagatagaaa aaatcaatgc acatgatctt
    61261 atccaaaaaa caggggtaca aattaattga aaaagccttg agatgaaagt tcctaaataa
    61321 aaaagttcag aatgataggt atttctggct tatagtataa tatttacgcc acagcctccc
    61381 aatgattctt tttggaatca taatcaagga tggacattca caaaaatatt ttgctcattc
    61441 gatccgaata gattaacgac caatctagta agtaatatag attggtacgt agtcaagctg
    61501 cacataattt agatcaattt tagttctttt ttatatttct atgatctgct catttacttt
    61561 tatatgatgg cttacagtaa cataaataaa tatgataatc gctggtagtt tatccaagag
    61621 tactcaataa aatacaaaat ggattgttca agcgcctatc ataatgataa aatgaaacaa
    61681 ataaacgata caaggaggaa gagactttga gtaatggcag aacaattttt tcattgtaaa
    61741 aattgttcag ttgttaatct atgggatttc ggtagcatgg gcattttttt atagaggaat
    61801 cacaccaacc tggccgtttt atctcttgtt agtgggaata ttagtaggaa attttgcccc
    61861 tcttcactat cgtacgaact tttttccaac tgattctggc ctttttctta aaacaaagac
    61921 gcgaaaatta gaaaacatta ttgaaatgac aattacctgt ataatcattt tcttagttat
    61981 attcctaaac gtcttctaat aagaatctct taaaattagc ttgttacagt tagttaccta
    62041 gtttatataa cacagttatc ttatcttcct tatcaacatc ttttgccaac tctttgtctt
    62101 aaattcgaaa catataagca gagctacgaa caccaagact gtttcggctc tgttttttgc
    62161 atcaaattca ctcttatata gctgataatc tcggctcttt aaaagaaatt attttcgctt
    62221 cattttatta taaaaaggct aggaaccttg atgttatcag gttcctagcc tttatttaag
    62281 tattacccca cagacccttc catatagatt tttaaattac acactagttt aaactaaaac
    62341 aaacaaggaa aaacttacca tttaatcaaa aaattagctc atatgaatat attagaatgt
    62401 aaattagact ggtcaaaatc tggtcaaaaa agaggacgaa tacaggtagt cctctttttt
    62461 gttattttga tattctaaat cattttttat tggattattc ccttgttcga tatagaccca
    62521 aattgatcta tcaataatta tcaaactgag tcatatagag acaaaaattt caaaaatgac
    62581 ctgttaaatg gtcatcgaat atcatttaaa atcaatacga cggagtcaga cacggagtca
    62641 aaacaagccc gctgagagta aaaaatatac agtcccatag gtaaagtgcc acaggactgt
    62701 atattttttt atttgagatt acctgaaatg attccattta atttttatct cattttttcc
    62761 tactagttag tatcaaatac tctcctattt tctcaataaa ctattttggg ttacatggta
    62821 tctgaaacag ctaaaacctt gtcattatag caaaaaatca ctttatctcc gtttcatttc
    62881 agatacattt cacttaagcg gattccaaaa aaacaaaaga aaaaggaacc cctagttagc
    62941 ctatgctttt tattggttcc tctcaaaaca cggtcatata acaacggggt ggggtaaatg
    63001 aatgaaaaag gtggaggaaa cattattcaa tagctgtctt aaatacttcg ttataaaatg
    63061 tgaacaaata aaagacatct atccctattt tctacacttc cacttgacta ccatctttga
    63121 atcgtacaat tttcttaact cttctcgttt acctcaattc attctcaagt ggaatcaact
    63181 catcatctaa cattttacgc catgctcgct tcatttcttg tgggtattta aatggcttcg
    63241 cttctggatg tttggcttta tatccttcgt aatccttact gttgataatt gctttcatat
    63301 taggaattgg atcttcacct ggtagatatt gcagattaga agcatgtaat tctttctcag
    63361 aaacaaacca ctcattggaa aagacatgaa tcaaatcatc actcgcttgt gtaaaatagc
    63421 gacgtttaat gacaaagata tcttcattac tatctaaagt ccccacatca aatgcattta
    63481 aaatttgtcg gtaaacttct ttgactcctt ctgttttatt caatttagcc aaagcttttt
    63541 caatttcttc tcgtgcatct gaactattcg cagcataagt tcctagtaat tgatcaatat
    63601 aagttgaatc aatatcatac aacttagtgg tattttcact ataaaattcg atcgttgaaa
    63661 aatcttctcc ttctttttct ggttcttcct ctattaatga acccttcacg gtctcgtaca
    63721 cgccaatttg ttcttccaaa agttgaattt gattgttcaa agttggagat ttcaccatgt
    63781 catcattgta atcgtcataa gtcactaacg attcataagc acgattcaat tcttgaaatg
    63841 ctttgacgta ctcgatacgg atttcaagtg gcgtatgttc atcaatggat tccatggatg
    63901 aaacctcttt taacttacca tatgcttgtt tgaatctttt tttggattcc tgataacttg
    63961 gataaatcaa acctgattct tcttttgctt ttgaatacag ttttgtcgca tcctcgacat
    64021 tcttctccat tgtcgctggc ttacgaaacg tcacaatcaa tccttttgtc ttatcaggat
    64081 acgtacgatt cgtacgtgaa aaagcttgga tcaatcctgc atactctaga ttacgatcca
    64141 caaatagggt ttgaatcgta ggggcgtcaa aacctgttaa tagacgatcc actacaatcg
    64201 ctaaatcgat ttgtttccca aattgtttga attccgctct ttttcgtgcc aaacgattat
    64261 tgatgtctcc gttataacgc tcaatgtctg ctaatgacca ggaagtatca taatactgat
    64321 tatattcctc aataattttt tgcatttctt cttgttgggc acttgaatct cggctatttt
    64381 cttctaatga ataggtaata gcgattcgtg gaaaatcagg atcctccatc gttcgtcctt
    64441 cacgaatgag ctgattggga aactcttttg ttaaccaatc aggctctttc gtcatctctt
    64501 tgatggcccg ataatacttt ttggccatgt caatggaact cgtggttaag attgcagatt
    64561 tcgttggcct gccatttcga aaatcaaatt ttgtataggc attatctgga cggaacattt
    64621 tatgaatgac tttttggata tgttcatctg cttcaaaaac agctgggtcc aaataacttt
    64681 cttttttgac aggttccatt tgatcaatca tacgattgat ctgttctgat gaatactctg
    64741 catatgtttc tacttctttt aatttccgat agattttatt ctctagtgaa gtcggctcca
    64801 cggtgttttc atgctctact tgaaatccta agacagagcc atcttctagc gcattcttaa
    64861 tggtataggt atgcaagacc tctccatact gatcatgggt tgtacgtgcc aattgccctt
    64921 tggcttgttt ccgattctca ggaaaaatcg gtgtacctgt aaagccaaac caagtggatt
    64981 tcggaaacat ttttttgatt tcttccatat tttcagcact
//
LOCUS       DARDHU010000005        60190 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000005 DARDHU010000000
VERSION     DARDHU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.005"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="SDD03_000371"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="SDD03_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG4302848.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="SDD03_000372"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="SDD03_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG4302849.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="SDD03_000373"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="SDD03_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG4302850.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="SDD03_000374"
     CDS             1640..2272
                     /locus_tag="SDD03_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG4302851.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="SDD03_000375"
     CDS             complement(2274..3179)
                     /locus_tag="SDD03_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4302852.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="SDD03_000376"
     CDS             complement(3264..3923)
                     /locus_tag="SDD03_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4302853.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="SDD03_000377"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="SDD03_000377"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG4302854.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="SDD03_000378"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="SDD03_000378"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG4302855.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="SDD03_000379"
     CDS             6166..7251
                     /locus_tag="SDD03_000379"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG4302856.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="SDD03_000380"
     CDS             7248..8312
                     /locus_tag="SDD03_000380"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG4302857.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="SDD03_000381"
     CDS             complement(8561..9364)
                     /locus_tag="SDD03_000381"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG4302858.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="SDD03_000382"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="SDD03_000382"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG4302859.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="SDD03_000383"
     CDS             complement(10242..11249)
                     /locus_tag="SDD03_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG4302860.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="SDD03_000384"
     CDS             complement(11256..11900)
                     /locus_tag="SDD03_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG4302861.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="SDD03_000385"
     CDS             complement(12000..12758)
                     /locus_tag="SDD03_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG4302862.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="SDD03_000386"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="SDD03_000386"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG4302863.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="SDD03_000387"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="SDD03_000387"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG4302864.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="SDD03_000388"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="SDD03_000388"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG4302865.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="SDD03_000389"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="SDD03_000389"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG4302866.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="SDD03_000390"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="SDD03_000390"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG4302867.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="SDD03_000391"
     CDS             18156..19025
                     /locus_tag="SDD03_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG4302868.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="SDD03_000392"
     CDS             complement(19151..20050)
                     /locus_tag="SDD03_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302869.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="SDD03_000393"
     CDS             complement(20215..21039)
                     /locus_tag="SDD03_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4302870.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="SDD03_000394"
     CDS             complement(21050..22345)
                     /locus_tag="SDD03_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG4302871.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="SDD03_000395"
     CDS             complement(22361..22840)
                     /locus_tag="SDD03_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG4302872.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="SDD03_000396"
     CDS             complement(22846..23874)
                     /locus_tag="SDD03_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG4302873.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="SDD03_000397"
     CDS             complement(23891..24997)
                     /locus_tag="SDD03_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG4302874.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="SDD03_000398"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="SDD03_000398"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG4302875.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="SDD03_000399"
     CDS             complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="SDD03_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG4302876.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="SDD03_000400"
     CDS             complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="SDD03_000400"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG4302877.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29968..30666
                     /locus_tag="SDD03_000401"
     CDS             29968..30666
                     /locus_tag="SDD03_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG4302878.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30834..33473)
                     /locus_tag="SDD03_000402"
     CDS             complement(30834..33473)
                     /locus_tag="SDD03_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4302879.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34092..35123)
                     /gene="queA"
                     /locus_tag="SDD03_000403"
     CDS             complement(34092..35123)
                     /gene="queA"
                     /locus_tag="SDD03_000403"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG4302880.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35316..36491
                     /locus_tag="SDD03_000404"
     CDS             35316..36491
                     /locus_tag="SDD03_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4302881.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36495..37136
                     /locus_tag="SDD03_000405"
     CDS             36495..37136
                     /locus_tag="SDD03_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4302882.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37133..38050
                     /locus_tag="SDD03_000406"
     CDS             37133..38050
                     /locus_tag="SDD03_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4302883.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38047..38718
                     /locus_tag="SDD03_000407"
     CDS             38047..38718
                     /locus_tag="SDD03_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4302884.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39259..39747)
                     /locus_tag="SDD03_000408"
     CDS             complement(39259..39747)
                     /locus_tag="SDD03_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG4302885.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39948..40214)
                     /locus_tag="SDD03_000409"
     CDS             complement(39948..40214)
                     /locus_tag="SDD03_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4302886.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40214..40477)
                     /locus_tag="SDD03_000410"
     CDS             complement(40214..40477)
                     /locus_tag="SDD03_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4302887.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40953..41138
                     /locus_tag="SDD03_000411"
     CDS             40953..41138
                     /locus_tag="SDD03_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4302888.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41280..43103)
                     /locus_tag="SDD03_000412"
     CDS             complement(41280..43103)
                     /locus_tag="SDD03_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG4302889.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43701..46406)
                     /locus_tag="SDD03_000413"
     CDS             complement(43701..46406)
                     /locus_tag="SDD03_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG4302890.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46712..47659)
                     /locus_tag="SDD03_000414"
     CDS             complement(46712..47659)
                     /locus_tag="SDD03_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG4302891.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47849..48118
                     /gene="rpsN"
                     /locus_tag="SDD03_000415"
     CDS             47849..48118
                     /gene="rpsN"
                     /locus_tag="SDD03_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG4302892.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48294..48656)
                     /locus_tag="SDD03_000416"
     CDS             complement(48294..48656)
                     /locus_tag="SDD03_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG4302893.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48667..49788)
                     /locus_tag="SDD03_000417"
     CDS             complement(48667..49788)
                     /locus_tag="SDD03_000417"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG4302894.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49804..50154)
                     /locus_tag="SDD03_000418"
     CDS             complement(49804..50154)
                     /locus_tag="SDD03_000418"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG4302895.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50284..51795)
                     /locus_tag="SDD03_000419"
     CDS             complement(50284..51795)
                     /locus_tag="SDD03_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4302896.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52117..53469)
                     /locus_tag="SDD03_000420"
     CDS             complement(52117..53469)
                     /locus_tag="SDD03_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG4302897.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53622..54698)
                     /locus_tag="SDD03_000421"
     CDS             complement(53622..54698)
                     /locus_tag="SDD03_000421"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG4302898.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54894..56639
                     /locus_tag="SDD03_000422"
     CDS             54894..56639
                     /locus_tag="SDD03_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG4302899.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56919..57356)
                     /locus_tag="SDD03_000423"
     CDS             complement(56919..57356)
                     /locus_tag="SDD03_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG4302900.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57340..58713)
                     /locus_tag="SDD03_000424"
     CDS             complement(57340..58713)
                     /locus_tag="SDD03_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302901.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59035..59523)
                     /locus_tag="SDD03_000425"
     CDS             complement(59035..59523)
                     /locus_tag="SDD03_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4302902.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59545..60147)
                     /locus_tag="SDD03_000426"
     CDS             complement(59545..60147)
                     /locus_tag="SDD03_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4302903.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17103 a        13016 c        10989 g        19082 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    27961 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    28021 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    28081 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    28141 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    28201 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    28261 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    28321 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    28381 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    28441 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    28501 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    28561 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    28621 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    28681 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    28741 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    28801 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    28861 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    28921 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    28981 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    29041 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    29101 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    29161 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    29221 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    29281 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    29341 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    29401 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    29461 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    29521 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    29581 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    29641 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    29701 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    29761 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    29821 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    29881 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    29941 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    30001 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    30061 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    30121 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    30181 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    30241 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    30301 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    30361 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    30421 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    30481 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    30541 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    30601 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    30661 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    30721 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    30781 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    30841 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    30901 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    30961 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    31021 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    31081 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    31141 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    31201 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    31261 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    31321 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    31381 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    31441 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    31501 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    31561 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    31621 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    31681 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    31741 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    31801 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    31861 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    31921 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    31981 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    32041 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    32101 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    32161 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    32221 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    32281 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    32341 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    32401 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    32461 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    32521 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    32581 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    32641 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    32701 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    32761 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    32821 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    32881 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    32941 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    33001 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    33061 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    33121 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    33181 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    33241 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    33301 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    33361 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    33421 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    33481 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    33541 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    33601 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    33661 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    33721 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    33781 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    33841 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    33901 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    33961 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    34021 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    34081 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    34141 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    34201 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    34261 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    34321 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    34381 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    34441 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    34501 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    34561 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    34621 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    34681 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    34741 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    34801 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    34861 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    34921 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    34981 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    35041 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    35101 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    35161 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    35221 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    35281 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    35341 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    35401 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    35461 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    35521 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    35581 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    35641 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    35701 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    35761 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    35821 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    35881 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    35941 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    36001 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    36061 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    36121 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    36181 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    36241 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    36301 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    36361 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    36421 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    36481 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    36541 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    36601 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    36661 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    36721 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    36781 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    36841 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    36901 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    36961 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    37021 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    37081 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    37141 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    37201 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    37261 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    37321 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    37381 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    37441 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    37501 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    37561 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    37621 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    37681 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    37741 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    37801 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    37861 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    37921 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    37981 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    38041 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    38101 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    38161 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    38221 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    38281 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    38341 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    38401 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    38461 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    38521 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    38581 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    38641 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    38701 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    38761 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    38821 cttatgggta aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    38881 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    38941 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    39001 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    39061 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    39121 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    39181 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    39241 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    39301 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    39361 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    39421 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    39481 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    39541 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    39601 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    39661 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    39721 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    39781 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    39841 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    39901 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    39961 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    40021 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    40081 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    40141 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    40201 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    40261 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    40321 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    40381 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    40441 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    40501 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    40561 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    40621 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    40681 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    40741 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    40801 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    40861 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    40921 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    40981 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    41041 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    41101 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    41161 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    41221 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    41281 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    41341 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    41401 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    41461 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    41521 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    41581 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    41641 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    41701 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    41761 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    41821 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    41881 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    41941 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    42001 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    42061 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    42121 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    42181 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    42241 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    42301 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    42361 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    42421 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    42481 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    42541 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    42601 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    42661 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    42721 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    42781 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    42841 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    42901 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    42961 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    43021 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    43081 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    43141 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    43201 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    43261 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    43321 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    43381 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    43441 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    43501 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    43561 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    43621 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    43681 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    43741 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    43801 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    43861 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    43921 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    43981 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    44041 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    44101 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    44161 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    44221 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    44281 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    44341 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    44401 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    44461 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    44521 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    44581 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    44641 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    44701 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    44761 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    44821 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    44881 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    44941 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    45001 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    45061 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    45121 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    45181 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    45241 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    45301 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    45361 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    45421 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    45481 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    45541 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    45601 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    45661 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    45721 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    45781 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    45841 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    45901 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    45961 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    46021 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    46081 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    46141 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    46201 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    46261 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    46321 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    46381 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    46441 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    46501 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    46561 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    46621 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    46681 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    46741 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    46801 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    46861 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    46921 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    46981 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    47041 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    47101 ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    47161 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    47221 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    47281 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    47341 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    47401 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    47461 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    47521 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    47581 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    47641 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    47701 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    47761 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    47821 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    47881 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    47941 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    48001 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    48061 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    48121 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    48181 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    48241 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    48301 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    48361 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    48421 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    48481 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    48541 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    48601 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    48661 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    48721 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    48781 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    48841 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    48901 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    48961 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    49021 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    49081 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    49141 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    49201 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    49261 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    49321 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    49381 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    49441 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    49501 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    49561 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    49621 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    49681 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    49741 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    49801 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    49861 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    49921 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    49981 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    50041 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    50101 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    50161 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    50221 aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta
    50281 tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc
    50341 gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata
    50401 attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta
    50461 tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc
    50521 tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc
    50581 taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc
    50641 gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg
    50701 taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt
    50761 tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg
    50821 gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga
    50881 aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt
    50941 aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc
    51001 acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc
    51061 gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt
    51121 ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc
    51181 tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg
    51241 cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt
    51301 ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt
    51361 ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt
    51421 acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc
    51481 gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg
    51541 tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc
    51601 tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg
    51661 tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg
    51721 cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag
    51781 ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg
    51841 ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg
    51901 tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca
    51961 ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca
    52021 agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct
    52081 aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc
    52141 taccacttca aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc
    52201 aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat agaacacagg
    52261 tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag
    52321 gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa
    52381 agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg
    52441 cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc
    52501 gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga
    52561 aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt
    52621 atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc
    52681 tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc
    52741 gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag
    52801 caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc
    52861 cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat
    52921 cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc
    52981 gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt
    53041 gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc
    53101 agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc
    53161 aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa
    53221 aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca
    53281 aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt
    53341 accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt
    53401 ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt
    53461 tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg
    53521 ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga
    53581 acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa
    53641 aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca
    53701 ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct
    53761 ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac
    53821 cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt
    53881 tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat
    53941 tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga
    54001 acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg
    54061 attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt
    54121 tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa
    54181 agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta
    54241 agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg
    54301 attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat
    54361 gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa
    54421 acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc
    54481 tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct
    54541 gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg
    54601 taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc
    54661 tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta
    54721 aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat
    54781 ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga
    54841 ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc
    54901 aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag
    54961 tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag
    55021 gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg
    55081 ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg
    55141 tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt
    55201 caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg
    55261 gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc
    55321 ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg
    55381 atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa
    55441 aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca
    55501 gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg
    55561 gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa
    55621 ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac
    55681 aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg
    55741 tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag
    55801 agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata
    55861 ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac
    55921 tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc
    55981 ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa
    56041 gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca
    56101 cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt
    56161 tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc
    56221 tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag
    56281 atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc
    56341 ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg
    56401 aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt
    56461 tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg
    56521 tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg
    56581 tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga
    56641 aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt
    56701 ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga
    56761 gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac
    56821 atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa
    56881 taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac
    56941 gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg
    57001 ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct
    57061 tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa
    57121 gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta
    57181 taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct
    57241 ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct
    57301 aattttatga catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt
    57361 tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt
    57421 gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag
    57481 cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt
    57541 catcaataaa aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat
    57601 ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc
    57661 tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa
    57721 gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa
    57781 acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa
    57841 atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa
    57901 taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg
    57961 caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa
    58021 gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa
    58081 aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt
    58141 tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc
    58201 catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa
    58261 ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc
    58321 catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag
    58381 ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac
    58441 gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt
    58501 gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc
    58561 ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa
    58621 taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa
    58681 attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata
    58741 actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat
    58801 tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg
    58861 attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc
    58921 aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa
    58981 accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc
    59041 gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag
    59101 ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg
    59161 attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg
    59221 aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa
    59281 ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt
    59341 taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata
    59401 agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa cttccggcca
    59461 tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc
    59521 catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag
    59581 catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa tctgcataaa
    59641 ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa
    59701 gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa
    59761 ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata
    59821 gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa
    59881 aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa
    59941 aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc
    60001 aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc
    60061 ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt
    60121 ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc atttattaaa
    60181 actgcctcct
//
LOCUS       DARDHU010000006        57233 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000006 DARDHU010000000
VERSION     DARDHU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.006"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            281..973
                     /locus_tag="SDD03_000427"
     CDS             281..973
                     /locus_tag="SDD03_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4302904.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1181..1471
                     /locus_tag="SDD03_000428"
     CDS             1181..1471
                     /locus_tag="SDD03_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302905.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2185..3555
                     /locus_tag="SDD03_000429"
     CDS             2185..3555
                     /locus_tag="SDD03_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302906.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3795..3878)
                     /locus_tag="SDD03_000430"
     tRNA            complement(3795..3878)
                     /locus_tag="SDD03_000430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3842..3844),aa:Leu,seq:gag)
     gene            3933..4112
                     /locus_tag="SDD03_000431"
     CDS             3933..4112
                     /locus_tag="SDD03_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302907.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            4261..5478
                     /locus_tag="SDD03_000432"
     CDS             4261..5478
                     /locus_tag="SDD03_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4302908.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5662..6498
                     /locus_tag="SDD03_000433"
     CDS             5662..6498
                     /locus_tag="SDD03_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4302909.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6574..7722
                     /gene="nagA"
                     /locus_tag="SDD03_000434"
     CDS             6574..7722
                     /gene="nagA"
                     /locus_tag="SDD03_000434"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG4302910.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7942..9000
                     /locus_tag="SDD03_000435"
     CDS             7942..9000
                     /locus_tag="SDD03_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG4302911.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            9004..10038
                     /locus_tag="SDD03_000436"
     CDS             9004..10038
                     /locus_tag="SDD03_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG4302912.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(10140..10856)
                     /locus_tag="SDD03_000437"
     CDS             complement(10140..10856)
                     /locus_tag="SDD03_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4302913.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10825..11610)
                     /locus_tag="SDD03_000438"
     CDS             complement(10825..11610)
                     /locus_tag="SDD03_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG4302914.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11601..12350)
                     /locus_tag="SDD03_000439"
     CDS             complement(11601..12350)
                     /locus_tag="SDD03_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4302915.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12648..13172
                     /locus_tag="SDD03_000440"
     CDS             12648..13172
                     /locus_tag="SDD03_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG4302916.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13188..14687
                     /locus_tag="SDD03_000441"
     CDS             13188..14687
                     /locus_tag="SDD03_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG4302917.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14775..15377)
                     /locus_tag="SDD03_000442"
     CDS             complement(14775..15377)
                     /locus_tag="SDD03_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4302918.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15481..16437
                     /locus_tag="SDD03_000443"
     CDS             15481..16437
                     /locus_tag="SDD03_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG4302919.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16742..17698)
                     /locus_tag="SDD03_000444"
     CDS             complement(16742..17698)
                     /locus_tag="SDD03_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4302920.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17872..18432
                     /locus_tag="SDD03_000445"
     CDS             17872..18432
                     /locus_tag="SDD03_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302921.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18425..19249
                     /locus_tag="SDD03_000446"
     CDS             18425..19249
                     /locus_tag="SDD03_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG4302922.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19259..19876
                     /locus_tag="SDD03_000447"
     CDS             19259..19876
                     /locus_tag="SDD03_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG4302923.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19894..20577
                     /locus_tag="SDD03_000448"
     CDS             19894..20577
                     /locus_tag="SDD03_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG4302924.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21260..22495
                     /locus_tag="SDD03_000449"
                     /pseudo
     CDS             21260..22495
                     /locus_tag="SDD03_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            22503..23390
                     /locus_tag="SDD03_000450"
     CDS             22503..23390
                     /locus_tag="SDD03_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4302925.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23407..24243
                     /locus_tag="SDD03_000451"
     CDS             23407..24243
                     /locus_tag="SDD03_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4302926.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24246..24869
                     /locus_tag="SDD03_000452"
     CDS             24246..24869
                     /locus_tag="SDD03_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302927.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24866..26158
                     /locus_tag="SDD03_000453"
     CDS             24866..26158
                     /locus_tag="SDD03_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4302928.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26517..26614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26730..27311
                     /locus_tag="SDD03_000454"
     CDS             26730..27311
                     /locus_tag="SDD03_000454"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG4302929.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27314..28621
                     /locus_tag="SDD03_000455"
     CDS             27314..28621
                     /locus_tag="SDD03_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG4302930.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28914..29402
                     /gene="purE"
                     /locus_tag="SDD03_000456"
     CDS             28914..29402
                     /gene="purE"
                     /locus_tag="SDD03_000456"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG4302931.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29395..30519
                     /gene="purK"
                     /locus_tag="SDD03_000457"
     CDS             29395..30519
                     /gene="purK"
                     /locus_tag="SDD03_000457"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG4302932.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30532..31827
                     /gene="purB"
                     /locus_tag="SDD03_000458"
     CDS             30532..31827
                     /gene="purB"
                     /locus_tag="SDD03_000458"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG4302933.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(32007..32384)
                     /locus_tag="SDD03_000459"
     CDS             complement(32007..32384)
                     /locus_tag="SDD03_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302934.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32640..33368
                     /locus_tag="SDD03_000460"
     CDS             32640..33368
                     /locus_tag="SDD03_000460"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG4302935.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33368..33622
                     /gene="purS"
                     /locus_tag="SDD03_000461"
     CDS             33368..33622
                     /gene="purS"
                     /locus_tag="SDD03_000461"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG4302936.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33624..34295
                     /gene="purQ"
                     /locus_tag="SDD03_000462"
     CDS             33624..34295
                     /gene="purQ"
                     /locus_tag="SDD03_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG4302937.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34296..36518
                     /gene="purL"
                     /locus_tag="SDD03_000463"
     CDS             34296..36518
                     /gene="purL"
                     /locus_tag="SDD03_000463"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG4302938.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36503..37942
                     /locus_tag="SDD03_000464"
     CDS             36503..37942
                     /locus_tag="SDD03_000464"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG4302939.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37965..39017
                     /locus_tag="SDD03_000465"
     CDS             37965..39017
                     /locus_tag="SDD03_000465"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG4302940.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            39014..39592
                     /gene="purN"
                     /locus_tag="SDD03_000466"
     CDS             39014..39592
                     /gene="purN"
                     /locus_tag="SDD03_000466"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG4302941.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39589..41124
                     /gene="purH"
                     /locus_tag="SDD03_000467"
     CDS             39589..41124
                     /gene="purH"
                     /locus_tag="SDD03_000467"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG4302942.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41140..42414
                     /gene="purD"
                     /locus_tag="SDD03_000468"
     CDS             41140..42414
                     /gene="purD"
                     /locus_tag="SDD03_000468"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG4302943.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42536..43450
                     /locus_tag="SDD03_000469"
     CDS             42536..43450
                     /locus_tag="SDD03_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4302944.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43583..45148)
                     /locus_tag="SDD03_000470"
     CDS             complement(43583..45148)
                     /locus_tag="SDD03_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4302945.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45141..46592)
                     /locus_tag="SDD03_000471"
     CDS             complement(45141..46592)
                     /locus_tag="SDD03_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4302946.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46606..47622)
                     /locus_tag="SDD03_000472"
     CDS             complement(46606..47622)
                     /locus_tag="SDD03_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4302947.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47842..48930
                     /locus_tag="SDD03_000473"
     CDS             47842..48930
                     /locus_tag="SDD03_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG4302948.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48984..50510
                     /locus_tag="SDD03_000474"
     CDS             48984..50510
                     /locus_tag="SDD03_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG4302949.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50512..51717
                     /locus_tag="SDD03_000475"
     CDS             50512..51717
                     /locus_tag="SDD03_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4302950.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51974..52951
                     /gene="guaC"
                     /locus_tag="SDD03_000476"
     CDS             51974..52951
                     /gene="guaC"
                     /locus_tag="SDD03_000476"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG4302951.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53215..54639
                     /locus_tag="SDD03_000477"
     CDS             53215..54639
                     /locus_tag="SDD03_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG4302952.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            55100..56209
                     /gene="pdhA"
                     /locus_tag="SDD03_000478"
     CDS             55100..56209
                     /gene="pdhA"
                     /locus_tag="SDD03_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG4302953.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56212..57189
                     /locus_tag="SDD03_000479"
     CDS             56212..57189
                     /locus_tag="SDD03_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG4302954.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        18451 a         9703 c        12410 g        16669 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     1081 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     1141 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     1201 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1261 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1321 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1381 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1441 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1501 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1561 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1621 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1681 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1741 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1801 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1861 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1921 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1981 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     2041 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     2101 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     2161 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     2221 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2281 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2341 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2401 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2461 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2521 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2581 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2641 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2701 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2761 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2821 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2881 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2941 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     3001 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     3061 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     3121 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     3181 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3241 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3301 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3361 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3421 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3481 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3541 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3601 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3661 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3721 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3781 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3841 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3901 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3961 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     4021 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     4081 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     4141 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     4201 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4261 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4321 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4381 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4441 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4501 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4561 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4621 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4681 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4741 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4801 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4861 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4921 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4981 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     5041 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     5101 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     5161 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     5221 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5281 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5341 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5401 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5461 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5521 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5581 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5641 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5701 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5761 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5821 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5881 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5941 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     6001 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     6061 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     6121 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     6181 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6241 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6301 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6361 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6421 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6481 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6541 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6601 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6661 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6721 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6781 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6841 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6901 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6961 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     7021 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     7081 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     7141 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     7201 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7261 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7321 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7381 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7441 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7501 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7561 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7621 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7681 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7741 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7801 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7861 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7921 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7981 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     8041 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     8101 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     8161 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     8221 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8281 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8341 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8401 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8461 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8521 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8581 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8641 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8701 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8761 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8821 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8881 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8941 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     9001 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     9061 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     9121 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     9181 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9241 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9301 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9361 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9421 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9481 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9541 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9601 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9661 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9721 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9781 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9841 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9901 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9961 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
    10021 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
    10081 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
    10141 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
    10201 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10261 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10321 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10381 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10441 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10501 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10561 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10621 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10681 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10741 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10801 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10861 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10921 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10981 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    11041 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    11101 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    11161 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    11221 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11281 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11341 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11401 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11461 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11521 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11581 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11641 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11701 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11761 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11821 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11881 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11941 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    12001 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    12061 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    12121 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    12181 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12241 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12301 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12361 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12421 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12481 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12541 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12601 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12661 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12721 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12781 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12841 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12901 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12961 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    13021 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    13081 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    13141 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    13201 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13261 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13321 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13381 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13441 tggacgtctc cacccctgaa gtgtataccg aacaaggcgt accggtgatg tgtgatggta
    13501 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13561 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13621 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13681 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13741 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13801 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13861 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13921 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13981 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    14041 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    14101 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    14161 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    14221 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14281 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14341 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14401 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14461 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14521 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14581 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14641 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14701 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14761 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14821 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14881 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14941 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    15001 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    15061 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    15121 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    15181 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15241 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15301 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15361 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15421 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15481 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15541 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15601 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15661 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15721 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15781 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15841 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15901 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15961 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    16021 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    16081 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    16141 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    16201 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16261 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16321 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16381 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16441 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16501 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16561 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16621 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16681 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16741 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16801 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16861 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16921 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16981 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    17041 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    17101 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    17161 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    17221 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17281 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17341 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17401 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17461 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17521 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17581 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17641 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17701 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17761 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17821 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17881 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17941 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    18001 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    18061 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    18121 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    18181 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18241 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18301 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18361 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18421 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18481 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18541 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18601 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18661 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18721 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18781 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18841 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18901 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18961 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    19021 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    19081 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    19141 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    19201 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19261 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19321 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19381 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19441 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19501 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19561 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19621 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19681 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19741 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19801 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19861 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19921 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19981 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    20041 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    20101 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    20161 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    20221 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20281 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20341 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20401 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20461 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20521 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20581 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20641 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20701 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20761 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20821 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20881 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20941 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    21001 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    21061 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    21121 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    21181 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21241 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21301 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21361 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21421 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21481 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21541 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21601 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21661 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21721 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21781 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21841 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21901 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21961 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    22021 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    22081 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    22141 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    22201 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22261 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22321 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22381 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22441 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22501 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22561 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22621 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22681 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22741 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22801 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22861 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22921 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22981 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    23041 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    23101 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    23161 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    23221 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23281 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23341 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23401 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23461 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23521 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23581 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23641 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23701 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23761 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23821 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23881 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23941 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    24001 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    24061 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    24121 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    24181 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24241 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24301 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24361 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24421 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24481 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24541 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24601 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24661 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24721 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24781 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24841 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24901 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24961 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    25021 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    25081 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    25141 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    25201 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25261 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25321 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25381 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25441 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25501 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25561 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25621 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25681 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25741 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25801 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25861 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25921 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25981 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    26041 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    26101 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    26161 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    26221 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26281 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26341 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26401 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26461 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26521 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26581 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26641 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26701 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26761 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26821 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26881 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26941 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    27001 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    27061 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    27121 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    27181 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27241 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27301 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27361 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27421 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27481 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27541 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27601 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27661 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27721 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27781 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27841 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27901 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27961 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    28021 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    28081 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    28141 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    28201 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28261 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28321 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28381 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28441 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28501 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28561 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28621 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28681 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28741 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28801 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28861 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28921 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28981 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    29041 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    29101 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    29161 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    29221 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29281 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29341 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29401 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29461 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29521 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29581 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29641 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29701 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29761 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29821 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29881 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29941 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    30001 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    30061 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    30121 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    30181 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30241 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30301 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30361 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30421 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30481 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30541 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30601 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30661 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30721 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30781 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30841 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30901 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30961 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    31021 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    31081 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    31141 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    31201 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31261 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31321 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31381 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31441 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31501 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31561 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31621 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31681 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31741 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31801 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31861 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31921 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31981 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    32041 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    32101 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    32161 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    32221 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32281 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32341 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32401 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32461 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32521 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32581 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32641 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32701 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32761 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32821 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32881 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32941 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    33001 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    33061 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    33121 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    33181 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33241 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33301 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33361 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33421 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33481 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33541 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33601 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33661 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33721 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33781 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33841 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33901 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33961 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    34021 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    34081 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    34141 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    34201 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34261 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34321 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34381 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34441 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34501 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34561 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34621 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34681 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34741 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34801 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34861 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34921 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34981 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    35041 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    35101 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    35161 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    35221 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35281 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35341 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35401 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35461 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35521 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35581 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35641 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35701 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35761 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35821 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35881 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35941 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    36001 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    36061 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    36121 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    36181 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36241 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36301 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36361 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36421 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36481 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36541 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36601 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36661 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36721 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36781 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36841 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36901 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36961 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    37021 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    37081 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    37141 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    37201 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37261 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37321 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37381 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37441 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37501 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37561 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37621 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37681 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37741 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37801 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37861 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37921 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37981 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    38041 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    38101 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    38161 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    38221 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38281 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38341 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38401 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38461 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38521 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38581 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38641 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38701 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38761 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38821 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38881 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38941 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    39001 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    39061 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    39121 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    39181 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39241 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39301 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39361 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39421 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39481 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39541 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39601 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39661 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39721 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39781 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39841 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39901 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39961 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    40021 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    40081 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    40141 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    40201 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40261 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40321 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40381 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40441 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40501 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40561 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40621 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40681 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40741 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40801 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40861 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40921 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40981 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    41041 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    41101 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    41161 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    41221 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41281 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41341 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41401 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41461 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41521 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41581 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41641 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41701 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41761 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41821 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41881 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41941 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    42001 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    42061 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    42121 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    42181 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42241 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42301 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42361 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42421 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42481 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42541 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42601 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42661 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42721 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42781 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42841 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42901 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42961 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    43021 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    43081 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    43141 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    43201 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43261 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43321 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43381 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43441 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43501 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43561 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43621 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43681 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43741 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43801 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43861 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43921 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43981 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    44041 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    44101 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    44161 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    44221 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44281 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44341 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44401 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44461 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44521 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44581 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44641 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44701 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44761 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44821 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44881 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44941 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    45001 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    45061 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    45121 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    45181 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45241 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45301 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45361 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45421 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45481 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45541 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45601 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45661 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45721 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45781 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45841 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45901 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45961 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    46021 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    46081 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    46141 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    46201 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46261 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46321 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46381 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46441 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46501 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46561 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46621 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46681 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46741 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46801 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46861 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46921 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46981 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    47041 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    47101 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    47161 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    47221 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47281 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47341 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47401 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47461 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47521 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47581 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47641 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47701 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47761 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47821 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47881 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47941 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    48001 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    48061 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    48121 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    48181 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48241 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48301 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48361 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48421 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48481 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48541 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48601 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48661 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48721 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48781 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48841 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48901 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48961 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    49021 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    49081 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    49141 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    49201 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49261 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49321 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49381 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49441 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49501 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49561 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49621 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49681 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49741 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49801 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49861 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49921 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49981 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    50041 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    50101 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    50161 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    50221 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50281 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50341 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50401 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50461 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50521 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50581 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50641 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50701 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50761 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50821 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50881 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50941 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    51001 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    51061 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    51121 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    51181 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51241 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51301 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51361 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51421 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51481 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51541 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51601 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51661 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51721 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51781 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51841 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51901 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51961 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    52021 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    52081 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    52141 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    52201 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52261 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52321 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52381 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52441 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52501 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52561 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52621 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52681 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52741 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52801 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52861 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52921 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52981 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    53041 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    53101 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    53161 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    53221 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53281 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53341 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53401 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53461 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53521 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53581 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53641 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53701 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53761 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53821 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53881 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53941 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    54001 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    54061 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    54121 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    54181 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54241 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54301 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54361 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54421 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54481 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54541 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54601 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54661 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54721 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54781 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54841 taagtttgat tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg
    54901 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54961 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    55021 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    55081 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    55141 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    55201 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55261 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55321 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55381 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55441 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55501 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55561 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55621 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55681 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55741 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55801 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55861 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55921 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55981 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    56041 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    56101 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    56161 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    56221 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56281 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56341 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56401 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56461 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56521 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56581 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56641 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56701 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56761 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56821 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56881 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56941 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    57001 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    57061 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    57121 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    57181 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DARDHU010000007        57220 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000007 DARDHU010000000
VERSION     DARDHU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.007"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..229)
                     /locus_tag="SDD03_000480"
     CDS             complement(<1..229)
                     /locus_tag="SDD03_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG4302955.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFK"
     gene            923..1309
                     /locus_tag="SDD03_000481"
     CDS             923..1309
                     /locus_tag="SDD03_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4302956.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1403..1594
                     /locus_tag="SDD03_000482"
     CDS             1403..1594
                     /locus_tag="SDD03_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302957.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2108..2440
                     /locus_tag="SDD03_000483"
     CDS             2108..2440
                     /locus_tag="SDD03_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4302958.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2736..2891
                     /locus_tag="SDD03_000484"
     CDS             2736..2891
                     /locus_tag="SDD03_000484"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG4302959.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3167..4507
                     /locus_tag="SDD03_000485"
                     /pseudo
     CDS             3167..4507
                     /locus_tag="SDD03_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4748..6364)
                     /locus_tag="SDD03_000486"
     CDS             complement(4748..6364)
                     /locus_tag="SDD03_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG4302960.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6482..6700
                     /locus_tag="SDD03_000487"
     CDS             6482..6700
                     /locus_tag="SDD03_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302961.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6895..7356
                     /locus_tag="SDD03_000488"
     CDS             6895..7356
                     /locus_tag="SDD03_000488"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4302962.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7549..7788)
                     /locus_tag="SDD03_000489"
     CDS             complement(7549..7788)
                     /locus_tag="SDD03_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302963.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7812..9335)
                     /locus_tag="SDD03_000490"
     CDS             complement(7812..9335)
                     /locus_tag="SDD03_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG4302964.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9353..9475)
                     /locus_tag="SDD03_000491"
     CDS             complement(9353..9475)
                     /locus_tag="SDD03_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG4302965.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9505..10488)
                     /locus_tag="SDD03_000492"
     CDS             complement(9505..10488)
                     /locus_tag="SDD03_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG4302966.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10512..10661)
                     /gene="rpmG"
                     /locus_tag="SDD03_000493"
     CDS             complement(10512..10661)
                     /gene="rpmG"
                     /locus_tag="SDD03_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4302967.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10682..11059)
                     /locus_tag="SDD03_000494"
     CDS             complement(10682..11059)
                     /locus_tag="SDD03_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4302968.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11091..11270)
                     /gene="rpmF"
                     /locus_tag="SDD03_000495"
     CDS             complement(11091..11270)
                     /gene="rpmF"
                     /locus_tag="SDD03_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG4302969.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11285..11554)
                     /gene="rpsN"
                     /locus_tag="SDD03_000496"
     CDS             complement(11285..11554)
                     /gene="rpsN"
                     /locus_tag="SDD03_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG4302970.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(12077..13843)
                     /locus_tag="SDD03_000497"
     CDS             complement(12077..13843)
                     /locus_tag="SDD03_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4302971.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13803..15556)
                     /locus_tag="SDD03_000498"
                     /pseudo
     CDS             complement(13803..15556)
                     /locus_tag="SDD03_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(15666..16235)
                     /locus_tag="SDD03_000499"
     CDS             complement(15666..16235)
                     /locus_tag="SDD03_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG4302972.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16253..17668)
                     /locus_tag="SDD03_000500"
     CDS             complement(16253..17668)
                     /locus_tag="SDD03_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4302973.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17679..18347)
                     /locus_tag="SDD03_000501"
     CDS             complement(17679..18347)
                     /locus_tag="SDD03_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG4302974.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18477..19061)
                     /locus_tag="SDD03_000502"
     CDS             complement(18477..19061)
                     /locus_tag="SDD03_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4302975.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19392..19625)
                     /locus_tag="SDD03_000503"
     CDS             complement(19392..19625)
                     /locus_tag="SDD03_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG4302976.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19640..20590)
                     /locus_tag="SDD03_000504"
     CDS             complement(19640..20590)
                     /locus_tag="SDD03_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG4302977.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20587..21429)
                     /locus_tag="SDD03_000505"
     CDS             complement(20587..21429)
                     /locus_tag="SDD03_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4302978.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21431..22150)
                     /locus_tag="SDD03_000506"
     CDS             complement(21431..22150)
                     /locus_tag="SDD03_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4302979.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22842..23000
                     /locus_tag="SDD03_000507"
     CDS             22842..23000
                     /locus_tag="SDD03_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4302980.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            23072..24298
                     /locus_tag="SDD03_000508"
     CDS             23072..24298
                     /locus_tag="SDD03_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4302981.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(24386..24778)
                     /gene="rpsI"
                     /locus_tag="SDD03_000509"
     CDS             complement(24386..24778)
                     /gene="rpsI"
                     /locus_tag="SDD03_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG4302982.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24794..25237)
                     /gene="rplM"
                     /locus_tag="SDD03_000510"
     CDS             complement(24794..25237)
                     /gene="rplM"
                     /locus_tag="SDD03_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG4302983.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25756..26223)
                     /locus_tag="SDD03_000511"
     CDS             complement(25756..26223)
                     /locus_tag="SDD03_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG4302984.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(26343..27881)
                     /locus_tag="SDD03_000512"
     CDS             complement(26343..27881)
                     /locus_tag="SDD03_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG4302985.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27961..28647
                     /locus_tag="SDD03_000513"
     CDS             27961..28647
                     /locus_tag="SDD03_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG4302986.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28848..29375)
                     /gene="msrA"
                     /locus_tag="SDD03_000514"
     CDS             complement(28848..29375)
                     /gene="msrA"
                     /locus_tag="SDD03_000514"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4302987.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29459..30136)
                     /gene="pgmB"
                     /locus_tag="SDD03_000515"
     CDS             complement(29459..30136)
                     /gene="pgmB"
                     /locus_tag="SDD03_000515"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG4302988.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(30133..31263)
                     /locus_tag="SDD03_000516"
     CDS             complement(30133..31263)
                     /locus_tag="SDD03_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4302989.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(31263..33446)
                     /locus_tag="SDD03_000517"
     CDS             complement(31263..33446)
                     /locus_tag="SDD03_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG4302990.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33443..34468)
                     /locus_tag="SDD03_000518"
     CDS             complement(33443..34468)
                     /locus_tag="SDD03_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4302991.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34513..35340)
                     /locus_tag="SDD03_000519"
     CDS             complement(34513..35340)
                     /locus_tag="SDD03_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4302992.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(35337..36404)
                     /locus_tag="SDD03_000520"
     CDS             complement(35337..36404)
                     /locus_tag="SDD03_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG4302993.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(36430..37266)
                     /locus_tag="SDD03_000521"
     CDS             complement(36430..37266)
                     /locus_tag="SDD03_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4302994.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(37263..38132)
                     /locus_tag="SDD03_000522"
     CDS             complement(37263..38132)
                     /locus_tag="SDD03_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4302995.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(38202..39485)
                     /locus_tag="SDD03_000523"
     CDS             complement(38202..39485)
                     /locus_tag="SDD03_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG4302996.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39499..41184)
                     /locus_tag="SDD03_000524"
     CDS             complement(39499..41184)
                     /locus_tag="SDD03_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG4302997.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41459..42484
                     /locus_tag="SDD03_000525"
     CDS             41459..42484
                     /locus_tag="SDD03_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4302998.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(43079..46753)
                     /gene="rpoC"
                     /locus_tag="SDD03_000526"
     CDS             complement(43079..46753)
                     /gene="rpoC"
                     /locus_tag="SDD03_000526"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG4302999.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46811..50428)
                     /gene="rpoB"
                     /locus_tag="SDD03_000527"
     CDS             complement(46811..50428)
                     /gene="rpoB"
                     /locus_tag="SDD03_000527"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG4303000.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            51156..52124
                     /locus_tag="SDD03_000528"
     CDS             51156..52124
                     /locus_tag="SDD03_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4303001.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            52142..53047
                     /locus_tag="SDD03_000529"
     CDS             52142..53047
                     /locus_tag="SDD03_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303002.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            53044..53850
                     /locus_tag="SDD03_000530"
     CDS             53044..53850
                     /locus_tag="SDD03_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4303003.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53986..54975
                     /locus_tag="SDD03_000531"
     CDS             53986..54975
                     /locus_tag="SDD03_000531"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG4303004.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(55292..55816)
                     /locus_tag="SDD03_000532"
     CDS             complement(55292..55816)
                     /locus_tag="SDD03_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG4303005.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            56103..>57220
                     /gene="scm"
                     /locus_tag="SDD03_000533"
     CDS             56103..>57220
                     /gene="scm"
                     /locus_tag="SDD03_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG4303006.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDT"
BASE COUNT        17345 a        11546 c         9710 g        18619 t
ORIGIN      
        1 ttttaaacat gtatagaaga aacatctcat taaaatcacc agtaaagaaa tggaaatctt
       61 tagttttttt aatattatca gttagtaact ttttataatc ataaaaaaaa taagtagttc
      121 tatctgagcg atactctttt ataatactga attcggaatt aaaaaccacg ataggtaact
      181 ttaaacaaac atgaagagac tttaataaga cttctttatc tgaaaacact tttgtttctc
      241 tctttccttt ctacatcatt aaatagtatt ttatacgtta tctaaaatac tattcctctg
      301 tcaaaataag ctaacttact tcattataaa atatccactt atcgctttca tgtatattcc
      361 accgtaaaaa gtacatttta tcaccgagaa ctcatcattt ttcatcatga tgaatactat
      421 ttacatatat ctagttcaaa gacaataatt attatgatcc gttattatct aagcactata
      481 cagataatag gaatttttcg tttctaaata ttccaatttg ggaatccaat aaaaattttc
      541 gttcaaatac gtgtctattc gaactctttt ctagttgtta attatgaagg gggaagaaat
      601 aaaaaataca atctatacac gaataagaat attatccaaa aggaggtttt agaaaagatt
      661 tattatacgt aaaaagctca agacattttc ttagccataa ggacatgttt gatatttaag
      721 aagatatatg aattttcttt tctaagtata aagataacta tccattatca actaaaaaaa
      781 catttttgaa aataaacatc agcagtccta tcaatgcatg tgagcttata gaagttattc
      841 gcagcgcagc ataattgcat tagcttcttt tcgtgttttt tgcctctttt ccctttaaat
      901 cttaatggag gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg cccagttgat
      961 tcgattcttt gaaaaggtca ttttgaacac cgctaatact tattataaaa aacaagaaga
     1021 aattcaaaaa catgaacaac ttgatcaact cccaccatat ttgtttacta aagatactat
     1081 aaaatttaaa caaccgatta atgttttaaa tgtgactctc cttgttgaag atataaaatt
     1141 agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg aaaaatttat
     1201 ctttgataaa actgataaag aaattggaaa ggttctgggg attacaagac agggtgcgac
     1261 aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat atctgcataa
     1321 taactaattt tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat agattataag
     1381 tccatgggaa gaaggtagat ttatgaattc tttaaaagaa gatttcattt tagcaaaggc
     1441 tggaaatgaa gaagctgttg aggcaattct caaaagattt agctctttga ttcataagca
     1501 atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc ttgcaatata
     1561 tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc tgtgacataa
     1621 gtaaaaagat agtttcaaat ttcgaacaat aaatttgaaa ctatcttctt tataattata
     1681 aagtatcgat tttacagcgt tctataatga ttcatagaat ctaaaacgaa tgattttaat
     1741 tatcaacgtt ttttaattcg tttttagctt aaaaataact tttgtcacag tctcactcct
     1801 aaaattactt tatttacgta tgaagtgggc aggaggtcga atccagaact gggctatggt
     1861 acttcaacga ggacttctcc tctagaattg aaaagcatgc gatttacctt ccgtaacgac
     1921 ggtagattta ttcaactatg tgtccaatca aaatttgtct gatttcaaaa atagtcaaga
     1981 gaattttggt tgttaaaaac tcttatctat caacttccac agtttagttg acaaaccctg
     2041 atactaagtt gggctatgat acacttttct tgactatcag ttacgttacc ggaaggaatg
     2101 aactctattg agaaaaacat atgatcgtaa atttaaacta aagatttcac aggatatcct
     2161 tgagaaaaaa gtaactacca agaaaattgc tgaagaatac aatatctctc gtccaacaat
     2221 ttcccgatgg gtttcagaat atcgtcgata cgggaaaaat gcttttgctg gacaaggaaa
     2281 aagattacct gataaagcag atttctacat tcttgaacag gaaaataaac gactaaaaga
     2341 ggaagacgag tgttctacga acacaaagat cgatacggat ctccagggat cactcaaaaa
     2401 ctttacgatg aaggaatcga aatcaataaa cgtgttgtag ctgttcttat gcgtgaaatg
     2461 aatttatgca caagaggatt tcatcgacgc aaatcttctc atggtagaga aaaaacaatc
     2521 aaagaaatgg taaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt gatgctgttt
     2581 gggtgatgga tattacttac attccatgta atgacggtcg cctctatttg tccacctata
     2641 ttgacttagc aactcgtatt ctacgttact atatggtaga ttcacacatg aaaaaagaga
     2701 ttgtcgtccg tccattacaa atttattaag gagaattgcc tacggtaatt cattccgata
     2761 gaggtagcca gcatcggtct tttatttatc aagagctgtt gaacgaccac cacatcacac
     2821 attcagtgag ccaaactggg actcctgttg acaatgcagt aattgaagcg tttcatcgat
     2881 caatcaaatg agaattaatc gactccaaca aatataaaag taaaatagag atgaaggtgt
     2941 taatccaaga ttatttagaa gactactacc caaacaaaag aatccataca aaatttatga
     3001 tgactccttg acaatttgaa gcgaagaaaa taagggatct ataatatcta gtgttggtga
     3061 cgccttctgt tgccaatact ttttcttttt tctaataaac gcaaacagac accttaaaat
     3121 cctttagaat aaagtcgacg aaaaccattc aaaggagtgt ttcaagatgt cctattcaga
     3181 gtttaccaaa gagttactag atattctaga cctaaatctt atctttcatg aagatgcctt
     3241 tcgcaaagag cgaatcaatg acgaaacatg ttttgttttt gatggcacat tgacctatca
     3301 accggaggaa tgctttcatc gccactatca aaatgaacaa acgatcatta aatagggctg
     3361 gaaaaaagta tccatcttat tgaatgatgt cagtaactac aagaccattc tgcggattaa
     3421 caaacagcgc ttcaaatgta aacactgtgg aaagactttt ttagcagaag attcagttag
     3481 tgatcgtcgc tgctccattg ctcgcagagt caaacaagcc attcttgagc tattaagcga
     3541 acctctttct atgtctttga tcgcacgtat gaagcacatt tctccgacca ctgtcattcg
     3601 aatacttcgc agccttcggc ctaaaaccat ttctttgagc cagctgttac cagaagtggt
     3661 gtgtttcgat gaattcaaat cagtgaaaaa tgtctcggga gccatgagct ttgtcatgat
     3721 ggacggcaag acccatcaac tgattgatat tgtggaaaat cggcaactca atcctttacg
     3781 cgactatttc ttgcgctacc ctataaaagt ccgtgaacaa gtgcgtctag tggtttcaga
     3841 tttctataca ccctatcgca cattggtgaa agagtgtttt ccgaatgccc gaatcgtcgc
     3901 cgatcgcttt catattagcc aacacattgg tcgggctttt accaaccacc gaattcaagt
     3961 aatgaagacc tttaaaaaag gggatcgtcg gtcgaagcac ttaaagaaat actggaaact
     4021 acttcaaaag aatgcgtggg aattgaaagg tcagcatcgc tactggcgtc cgtcatttcg
     4081 agatcatctg acagaagcag agatagtcga tcggctatta tcttatgatg attccttaaa
     4141 gcgaggctac gaagtctacc aggattttct ttccgttatc agaagacaag atgttcctga
     4201 attcgtcgcg ctcctaaaag aagactacaa ggagttgcca gagcactacc aaccagtgtt
     4261 cacgaccttc aagaaatacc aaacagaaat caaacgagcg ttgcgcgttc cctattccaa
     4321 cggccctata gaatgtttga ataaccatat caaagtacta aaacgaattg cctatggcta
     4381 tagaaacttt caaaactacc gagaacgaat ctttttatat cgaggaaaat attttaagaa
     4441 aactaagaat actacccaac tgactaaagc cagaacaaca acacggctag acaagatagc
     4501 tgtctagccg tgtgagtatc tcgattattc tattgaattt cttcaccaac actcattgac
     4561 gaagagccaa aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa
     4621 gatacttgtg cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa
     4681 taaagcccct gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat
     4741 ttccagatta gtaaatgtag taatctgtgc tttctttttt ctcaatacta ataactaact
     4801 tatggggtaa acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga
     4861 tttgatcagg actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat
     4921 tatattgtcc cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga
     4981 agcgatccat ttccttgcca tcaattcgaa cgatggcata tttgatagca tcttcagttg
     5041 tttcattctc cttttctata gagaatacaa ataagggaat aaaagaggta ataatcaaaa
     5101 tgccaataat tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg
     5161 atatctggct aaacatatta gactttttcc atggaaatgg tgaaattttt cggaaattat
     5221 ctgcattaag agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca
     5281 tcttcatacc aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt
     5341 ccttcaattc gggatttttt tgtataatca tttgacactc aattttaggt aaactagcta
     5401 cccaataatt taatgattca atacttttat agctacccga tgtttcaaag atcgtcgaaa
     5461 aatcactatg ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat
     5521 ttttatccgt aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa
     5581 aacttgagat accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact
     5641 ctttgaacca gcccactgaa taggaagtct catctatatt aaaaagattt gtatttagaa
     5701 ttttcgataa gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact
     5761 tagcaattag accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta
     5821 aaagtaatat gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt
     5881 cttcaactac attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat
     5941 cccaaaaacg ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca
     6001 aatcaatgtc taaagggttt ttagctaata taattgcttg aataattgga attggtttgc
     6061 tgattcctga agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta
     6121 gttcaacaat caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa
     6181 gtttttctcc agttctttta aaacctttct tgtcgactaa taccccatca acatttactt
     6241 cccctatgtc atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat
     6301 ttagaagtat cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt
     6361 tcatagggaa cctctttcta taccttgaat ttacacttta agtgcaacac ataaaaatga
     6421 gtcataatcg atatccaact agtataagtt tgtcgagaac actcctagaa ggaaaataat
     6481 tatgagccag tatcgtcatc ttactatact cgaacgagaa agaatctttc tgcttcacga
     6541 agaaggattt tctattagta taattggcaa cgacattcag catagcccgt ttactatttt
     6601 acatgaactg agaagaaaca agaaaaaaac tattcacctt ccaaagcgga aaaaaattat
     6661 gcttatcgaa gaaaaaaatg cggacgttat cacattctaa atgatccgtg agtttggtca
     6721 attgttcgtc atttatttgt cgattttcag tggtcgccag aggaaatctc gaatcgtttg
     6781 aagcatgaac atatggactt ttcaattagt tttatcacga tctaacgtgc aatttgcaat
     6841 gatcttttcg atacagaacc cctctcacac ggcaataaag gcctgatccg ttcattgcac
     6901 catcgaggaa aaacaagaca tacaaaaaat catgtcgagt ctcgtggaaa gatttgcctt
     6961 actcattcga tccatggccg tccaaaatcc gcagatgata gaagtgattt tggtcattgg
     7021 aaagccgata ttgtagctgg caaaactggt ggtacttgtt tagtgacttt gacagacaga
     7081 aaatcatgct tccttttagc aggaaagaag gtccccagaa agaaagccgc ttatgtgagc
     7141 gagaaaatga ttgaactttt aagcagtatt ccaaatgagg cggtcaaaac gatcactcca
     7201 gatagaggaa aagaattctc caatcatttc gcagttacag aagcattgaa taatatgccc
     7261 ttttattttc cagatcctca tgcccttggc aacaatgaac aaattaaaat acgaatagac
     7321 tcattcgaga atacttgcca aaatcgaaag agatagctac ggtagataac tcaacgataa
     7381 atcagatgat tgaaaaactg aataacagac ctagaaagtg tttagattgg agaacacctt
     7441 atgaagtctt ctttgataaa gtgttgcgct tgatttgaca attcaagaca taataaaaag
     7501 cagatcaaat ccaaataagg gaaaaatgat ctactctggt attatcaatt aattggccaa
     7561 gctattatgc cgttttgcta aacgtctact tcttccagta ttgaccttga taaaacaaca
     7621 atcaggatcc cgttttaata ctctatgaac tgtgtcaata ctaacatgta aagttgcagc
     7681 aatatcagct aatgtactcc catttaatct catttgcttt gcaaatcgat aacgccgata
     7741 agcatcaata ttcttggaat ccatagacaa taatactttt cttaccatgt atttcctcca
     7801 aaaattgaat tttaatgagc catcatttct tgagcaattt catgtttaga caatgaagct
     7861 ggatagaaag taggccaatt atgcaattca ttatagagtt tttcttgact ttctccacca
     7921 aagaaaatat ggaaatgctc tgccttactt ggtgcaattc catgatcact aaattgaata
     7981 tatttaaaac gaccagcacc tgcatcatca gtttcaaaat taaaacgaac accacggttt
     8041 cctttttcat aattcaaaat tttgtatcca acatatttgt atgtgtattg atgatgttca
     8101 ccgttgacta caaaatcaat ggtgttgtct ttaatgttga tagtatcaat atctgtctta
     8161 tagccagtag tataataatc tttgtactcg gcagctgtca tgtctttatt gatttttgct
     8221 ttgtaatcga aaacttcatc caaaattcca tcttttaaca acggataaac agattgccat
     8281 tcgcctgtat aatcactcaa ttcgcgatcc ttcacatcgg catctttaaa gtacccatta
     8341 gcaactgtct tttcatcctt cgcttctttt tctggaacta cctcttcacc agcaaccata
     8401 gttgtttttt ctaacgcttt tagattgtct tccattactg aaacataatc ttcaccatta
     8461 tccatttgtt cttttgtaag actttcaaga ggattcagta cttccaattt tatcccagct
     8521 tcgttggcca aagtttttgc aattttatca ttagcattct tttcaaagta aatataattg
     8581 atcttatttt ttttaacata ctcttttaat tcagctaatc gaccagaaga tggctcttct
     8641 tcaggactta gtccagcaat aggaacttgt ttcaatccat aatccaaagc taaatatcca
     8701 aaggctgcgt gttgcgtcac aaaacttttt tgtttcgctt ctttaagact agtggtatag
     8761 tcagcatcca accgtttcag tttagtcaaa tacttttcag catttgtctc aaatactttt
     8821 gcttttttcg gatacaattt aactaactga tccttgatat ttgaaacttc cttaattgcc
     8881 attttgggtg aaacccacgt atgaggatct agttcatgat ggtgatcttc ttctccgtga
     8941 tcatgatctt cttcttcact acctggcaat aaaatcatgt cttttgttcc ttctactaca
     9001 ttaggcccag ctttcttcca actttcttta gcttttggta cccatgattc catgttttcg
     9061 ttatgatata caaaaacatc agcatcatga gcttttgcca tatccttagc tgagggctca
     9121 tagtcatgag gttctgatcc cgctggaacc attaaatcca cgttcgcttc atcgccgacg
     9181 atgtttttgg tgaaatcata cattggatag aatgttgtta caatttctaa ttttccatct
     9241 ttatcattcg atacttttgc atcattagtg ctggagcaag ctcccaataa tcctagagct
     9301 gctatcccaa taagtcctac actgattttc ttcattcttt ttccttcctt aattatgcta
     9361 atttcttcat tttatttcgt ttatgttctt tgataatttt taatgccctt tttctagttt
     9421 taatattcgg gcttttaagt cgtcgatatg cactagatat atccgttttc tccattagaa
     9481 attaccccca atcactactt atcattattt gctttccgcc agtaatttat caaaactctg
     9541 tttgatcctt tcttcatcca acatttttcc gattagaata atagtggttt ggtctttttg
     9601 tcgagtggtg tattgaaaat taacctgttc gtttacctct tgcatggcaa ctataaaatc
     9661 ctgctcttgt aaagcaatca atcctttaaa acgatacagg ctcccttggt tggttaaaat
     9721 cagccagtca atccattgga gtaaaaaatc tttttttatg tcttgatctg aagttaagag
     9781 aattgattta aactcatgta cttgatggtg ttgatgatag tatttgccat tatagtgatt
     9841 ttctgatgaa tcactttcaa aaactaaggg atgattaaat tttttcaaat tataaaaatc
     9901 ctttttttga atcgtttcac tatgtgtgaa cattcgaata tcggataaag ggttaatact
     9961 tttgatttct ctctgtaatt tttttagtgc agataaattc aagttttgtt ttctagacac
    10021 aaaaatgcga tctgccatga caagttgctt aagggcttgt ggttctcttt caataagttc
    10081 aaaactgtca ctatcaataa cagtcaataa actatcgata tatactcttc ctcttagttg
    10141 aggcgctgtt aaaagtgttt gaatgattgg ttggggatct gctatacctg tagtttcaat
    10201 gatgatttgt gaaattggtt gttgatgttc ttctattgtt tccagtaaag aaatcaatat
    10261 tgctaataaa tcactgcgaa gactgcaaca catacatcca ttatttagtt gtaacacccg
    10321 ttcctcgcta tgaactaata atttgtgatc aatcccaact tcaccaaact cattttcaat
    10381 tataataatt tcttccctgg ggtagggtga gacttgcaag gcttgattga ttagcgttgt
    10441 ttttccagcc cctaaaaacc cagaaacgat tgtaactggt atgcccattt tgtcaccttt
    10501 ttaccactta gctatttcac ttctttaaaa attgcccgtc tgcgcaattt tggcgaatac
    10561 ttttttaatt ctaaccgttc agggttattc cgtttatttt tgctggtgag atacagacgt
    10621 tctccagttt cgacacattc caaaataata ttttgacgca taaatcttcc tccttcaata
    10681 cttatcgatc ttcaagatat gcttgaattt gttgtgcatc ttcaaaagtt aactcgccag
    10741 attccaattg tgaaactaaa ttttctttca tatcaacagg tatctcccaa taaccatcga
    10801 tacattgaaa taactcctcg tcatactctt caaaaattgg atagattctt aaaaaatcat
    10861 caaatgataa gccttggtcc tcatagtaat ctttgactaa tccattttca attgcctgtt
    10921 gggtcggtaa acccgtttca gtaataattc ctctctctac catcgaacga taaaaagtgc
    10981 atttatctag cgattgctgg gtcgtttcaa gataatactt taatccttct tcaactaaag
    11041 gtgggtaaat aatttccatt ttgattccct cctaaaagtt atatattaaa ctattttgaa
    11101 gctcctttaa ttactttctt tccattatag taccctttta aagatacatg atggctacgt
    11161 ctataatcac cattcgcttc gtcaaatgaa atttctggtt ttactacagt ttgatgagtt
    11221 cttcttaacc gtttcttcgc cttagatgtt tttcttgctg gtactgccat gatatttcct
    11281 ccttttacca acttgctttt tttactccag gaatcaaccc ttgatgggca agttctctaa
    11341 acttaatacg ggacataccg aactttcgca tataccctct tggacgacca tccgtctgat
    11401 cacgaagcct cagacgattc ggatttgaat ctcttggcaa tttagcgaga gcggtgcgat
    11461 ctcctgcggc ttttaattcc tgacgtttat ctgcatactt ttcaatcatc gccatttgtt
    11521 tctttgcttt agcaattttc gacttttttg ccatatacct ttatactcct tttattttga
    11581 tttagacacc tcaaatcgta atcgttacgt tttgacactg atactttacc aacaagagtt
    11641 gctcttgtca acaataaata acatgaaata aaactcatat gaatagataa ctatttgaaa
    11701 acgaatttat cggtacatag agataatcaa atcatttaaa agaatatgca gtttaggctc
    11761 tgaaaaataa gaattcactt ttattagtcc aatcaaacac aaataccgtt ttaagtagct
    11821 tttcctctac ttaaaacggc aatatgaaag aaactataac tttttattca atcttttatg
    11881 tttaactggt ttatcgccaa ttttttaaaa ctttgattat tccttatatt ataagaatag
    11941 acattaagtg aactaattca atcatactga ttctttcgtg tattcaaaat tagatgctgt
    12001 ttaataaaat tattcttttt gaaaaagaat tataactata tcccaaaaat tgtaggttaa
    12061 tttaaagcct atttcatcat cgtgattgag cttctttttc atagacgaat ttctcagata
    12121 gtttagtttt ctttatgaga tttttgtata tttctgacct tcgatacaac tcattatgag
    12181 taccttgatc aactagtttt ccttgatcca tcactacaat tttatttacc ttttcaatag
    12241 acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga tttaaacttt
    12301 cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct aaaattaaaa
    12361 ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct cctccagaaa
    12421 gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt tcaataaagt
    12481 cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct tgactatttc
    12541 caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga aaaacaatag
    12601 cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca tcaattaaaa
    12661 tacagccttc atcataatca tacagtctag acactaattt caagatacta gttttcccgg
    12721 aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa gagatatgat
    12781 ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca atgtcaaaag
    12841 atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct ctaattcgct
    12901 gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa tcgaaggaat
    12961 cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc acttcacccg
    13021 ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat gagaaaatag
    13081 aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc ctttcactct
    13141 cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag ctattaatct
    13201 cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat
    13261 atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca aaactaaata
    13321 acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc atcactgaaa
    13381 taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc atcgcatgct
    13441 caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta tgttttgaaa
    13501 aataggataa tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt
    13561 ctttataagt actattatat aagttttcat actccctatc taacaaccaa tacatgaaaa
    13621 gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg ctttgtattt tctaaaacta
    13681 attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag tatacaaaaa
    13741 aactagaaat agaagcttta gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt
    13801 ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata attagtgacc
    13861 aattgtttat acacttgatg tttagccatt aattcattat gatttccacg ttctactatt
    13921 ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa tcgatgagct
    13981 atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag ttcatactca
    14041 ttatctggat caatagatgc actggcttca tccataataa caatctttgc atcttttaaa
    14101 attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac tcctttacta
    14161 ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca accggctaat
    14221 cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga aacgttatcg
    14281 taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt gttaataatt
    14341 gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc cttatccaca
    14401 ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga ttttcctact
    14461 aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct
    14521 ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc
    14581 ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca
    14641 tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata
    14701 attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga
    14761 gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat
    14821 tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa
    14881 tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata
    14941 acttgaattc cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga
    15001 tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac accaattact
    15061 aacatggcga ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat
    15121 aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt
    15181 gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa
    15241 aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag
    15301 cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc tctaaagtta
    15361 accaagcaac agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta
    15421 gagactgaag aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat
    15481 cagaaaataa agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt
    15541 ttttgtatac tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa
    15601 ctgttaatgc tttccgtaat agcgtaaatt agagatgtac aaacagataa aaggtaaact
    15661 acgttctatt ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat
    15721 aaacttgcta caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt
    15781 gcatccattg ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta
    15841 aagaaccata taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac
    15901 gcaacttgag gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc
    15961 gccatactaa tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat
    16021 aacaataaaa caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata
    16081 taaacaaata tgattccaga taataatcca aaaatcccgg gggatatgct gtgaccaaac
    16141 gccccaccaa tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta
    16201 gctaaaccaa taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg
    16261 ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt
    16321 tttttattcc accatttttt aaataaataa ttctattgca tgttttataa agaaattccg
    16381 gatcatgagt agctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa
    16441 tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag
    16501 tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa
    16561 acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat
    16621 ctttactaac tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt
    16681 ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta
    16741 tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca
    16801 caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt
    16861 ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga
    16921 tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt
    16981 cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt
    17041 gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt
    17101 tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg
    17161 aagacggttc atccaatata attaatttag agtttagcga taaactacat ccaatagaaa
    17221 ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat
    17281 ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt
    17341 tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat
    17401 cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt
    17461 tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat
    17521 tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac
    17581 tgcctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg
    17641 aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa
    17701 aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa
    17761 gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca
    17821 gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc
    17881 tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca
    17941 gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt
    18001 aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac
    18061 catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat
    18121 atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa
    18181 taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta
    18241 gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga
    18301 aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca
    18361 aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag
    18421 tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt ttttaattaa
    18481 ctcagtaatt caatccaccc aggaacagaa aattgataca atcttctggt taatagcata
    18541 gtcttttctt ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa
    18601 atgtagaagt ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc
    18661 tttaacttat ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca
    18721 atatcttgat atttggttcc agctgattgg aataaaatca aatcgaatat acgtttattc
    18781 tcaaaaatat aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct
    18841 atatctatat tttctaagtt taagaggtat ttggtaaact cttcattata catattaata
    18901 agcgcattaa tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt
    18961 ttataaaaag cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc
    19021 tgaaatcctt ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata
    19081 atataatctc tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct
    19141 tacacaggac ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta
    19201 aactaatgag aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac
    19261 tcatctaaat cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc
    19321 agctattagg ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa
    19381 aataaagtct tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca
    19441 gttaattgaa atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt
    19501 acagatgctg gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt
    19561 ctttttgata attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt
    19621 accataccta ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt
    19681 tcatcatcgc atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa
    19741 tcgttgaata gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca
    19801 cactgctttc tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt
    19861 ttatttgatc tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca
    19921 cattatacgc tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat
    19981 cattcggaat tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa
    20041 tatacttatc tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact
    20101 gtttttcaat atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat
    20161 cttccttccc tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg
    20221 caaaatagtc tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat
    20281 ttcctccagt ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt
    20341 gaacatcttc aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt
    20401 gtaaatcaat tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa
    20461 ctactacttt taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac
    20521 atgccccgaa tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt
    20581 ttctcatcat aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa
    20641 actaattaca aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa
    20701 actataacca ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa
    20761 tatcatagtt ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat
    20821 tgcaacaatt aatatagttc ccacactttg catagctgtt actgctacta aagttaatag
    20881 aaccatcaat agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg
    20941 atcaaaagaa gtgattaata attctttaaa aaatagaact actataacca acactaaaac
    21001 cgaaacgcca attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa
    21061 aatatgaaat aaatccgttg agctatttgc aactcctata aggataactc ctagtgctaa
    21121 aaaagaactg aaagtaatac caattgctgt atctcctttt attacactat tgctttttat
    21181 aaaagtaata atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat
    21241 acctaaaata taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat
    21301 aagagacatt cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac
    21361 tacagatgta attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt
    21421 ttgaagcata ttattccccc tctccaataa atatagtatc tccataggct ttttttaaat
    21481 tttcttcatt aaatacagat tctacacttc cgtgagcaat tagatttcga ttcaaaagaa
    21541 taatattatc aaaatacttc tttaccttgc ttaaatcatg atgcacaatt agcactgttt
    21601 ttccctgatg tttcaatttt tttaacgtat ccataattat tcgttcacta actaagtcta
    21661 tccctacaaa tggttcatct aaaaatatga aatccgcatt ttgagcaaga catcttgcta
    21721 aaagtacacg ttgaaattgt cctccagata attctcctat ttgatgatta gaatattttc
    21781 ccatatcgac cttttccaaa gcctttgaaa ctctttgcca ttctgcattt ttaattcttt
    21841 gaaaaacttt cattccggca tatgttccca tagatacaca ttcttttact tttattggaa
    21901 aagtaaaatc aatatcagct ttttgctcta catatgcaat cttcaccagt tttttattga
    21961 tatcttcatt attaatggag acctctccct gatgagggat aatatttaac attgctttaa
    22021 gcaatgtgga ttttccagca ccatttggac caataatacc tgttaaagca ccttcttgta
    22081 tttttaaaga aatctcgttc aatgctaaaa aatcgttata acaaacatta agctttttca
    22141 cttctaacat aactttctcc ttctaaatga taatgattat cacgatcata tggtatacaa
    22201 aaattatatt ttagtcaacc ctaaatttta tagtttagat ttttttctgt tgctgaagat
    22261 tagaatgaca aaactatttg aaactacgcg tatagtaagt agctttcaaa atatataaaa
    22321 aaggagcgtt ctcaaatgag aacatgctcc taaaactact ttattggcgt atggaaaaat
    22381 tatacttgaa gtggatagcg ggttgaataa gttgataagc atcaaaacat ttataaaaat
    22441 tattcaagac tatgtctgtt ccatatacaa atatgaaaat cagtaaactc catttaactt
    22501 ttttcgtagt gtgtcttttt atacatgttt atcgttatta atagtgaata ggaaaaaaac
    22561 tatttttgat cattgacaat taaatacaat ttttgaataa cgatccctgc tattcgagct
    22621 agaaactcaa gtacgccatg acctgattag cgacaaatac ttagagattt ctttttgttc
    22681 tgggaaaaca aaatgccatg acaatagcag caaataatga tacttccgta aggctcgccg
    22741 taaactgggg agaggtgaaa ttcctatgtg cttacccaaa gcaattcaaa aatttcataa
    22801 ctactatttt aggagatgat tttatagaat ccagagtcta cctgaataaa aaacaaacct
    22861 gtgcatttct aggaatcgat aataatactc tagataagtg gattcgtata tataatttcc
    22921 cagtaataag agttggtggt gtagttcgct ttgacgtaga agatattaaa gactttatga
    22981 atacatttaa aataacttga aattttcttc tcgccgcata tactaaaatt agtgaaatat
    23041 actgccttac tttttttaag gagtgaggat tttgcctaca aagctttcaa atgggaaata
    23101 taaaactaat ctgagatatc caaaaaggtt taaagaaata acaggaattg catcagaaaa
    23161 gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa
    23221 gaaaatagaa aaagttttga gagaggagaa tgcaaactca ttagaattaa aaggaaaaat
    23281 aacgtttaaa aaattttacg aatctaaatg gcttcctaga tatgaattag gtcaaacaat
    23341 tagatcaaac aggcctccta gtgatataac cattagtaat acaaaagata tatttcggtt
    23401 gcatatttta cctatgtttg gtgaatacgc aatgaattat ctaaatatca atactgagat
    23461 aatatcagat gaattaacca aaaaatcgaa agaatatgca aatataaaaa tcataaaagg
    23521 atatgttcgt tccatgttcg atattgcaga aatactaaat tatatcgaat ttaatcgaac
    23581 tactaaaatt attcaaagta tcactgcacc taaaaaaaat gctttagaag aaaaaagaat
    23641 acaagaaggt aagcaagcac tatcatcaaa agaacttact aattggatag aagcagtaaa
    23701 tgatgatttt aataatcatt tacttacttt tcatgattac acacttttta tgattaccct
    23761 gtatcttggt gatagaaaaa gtgagactta cgctttgcaa tggaaatata ttgattttga
    23821 aaaacaaacg gtccgtctta aacatacttt agataaatat caaagaaaaa agtttacaaa
    23881 aggtcgaaaa gataccgtca ttcaagtacc agaagttgta atgacattgc taagtgaatg
    23941 gaaatcggta caagctgatc aattgttgaa attaaaaatt aagcaaacgt tagatcagta
    24001 tctctttact tatactaaac catctggaga agtaaattgt cctgttcatg ccgattacct
    24061 aaactatcga atcaattcaa ttaaaagaag gcatcctgac ttagttcatt tatctccaca
    24121 caaactccgt catacttatg caactatagc tagacaaggt ggagcagata tgaatcaaat
    24181 ttcaaatgct ttaacacact cagatatttc tacaacaaag atatatgtga acacaccaga
    24241 tatagttgat aaggctgttt ttgaagcttt tcagagagga ttgaataagt gtgattgata
    24301 tgtgttctta aatgtgttct taaaaacaaa agaagcactt tccaaataat tgaaaagtgc
    24361 cagaaacgtt gatatataaa ggatattaac gttttgagaa ttgtgaagct ttacgtgctt
    24421 tcttaagacc tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac
    24481 gtttaagagc taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac
    24541 ggatagctcc tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg
    24601 cgccttttgt ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg
    24661 gaatgtattc ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac
    24721 gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg
    24781 aaattccctc ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat
    24841 gtggatgttc tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt
    24901 ttttaggaag catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca
    24961 attcgccagc agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt
    25021 tgtcagtcgc ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat
    25081 cgccagtatc caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa
    25141 caactgttga aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt
    25201 ctacttcgcc tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt
    25261 ctgatgtttg acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt
    25321 aacatattac ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat
    25381 atgaaacttt acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa
    25441 aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt
    25501 ctcctgccaa ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt
    25561 aaaattgttt cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg
    25621 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg
    25681 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
    25741 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
    25801 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
    25861 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
    25921 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
    25981 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
    26041 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
    26101 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
    26161 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
    26221 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
    26281 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
    26341 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
    26401 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
    26461 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
    26521 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
    26581 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
    26641 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
    26701 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
    26761 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
    26821 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
    26881 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
    26941 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
    27001 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
    27061 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
    27121 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
    27181 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
    27241 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
    27301 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
    27361 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
    27421 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
    27481 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
    27541 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
    27601 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
    27661 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
    27721 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
    27781 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
    27841 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
    27901 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
    27961 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
    28021 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
    28081 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
    28141 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
    28201 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
    28261 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
    28321 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
    28381 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
    28441 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
    28501 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
    28561 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
    28621 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
    28681 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
    28741 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
    28801 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
    28861 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
    28921 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
    28981 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
    29041 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
    29101 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
    29161 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
    29221 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
    29281 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
    29341 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
    29401 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
    29461 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
    29521 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
    29581 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
    29641 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
    29701 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
    29761 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
    29821 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
    29881 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
    29941 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
    30001 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
    30061 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
    30121 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
    30181 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
    30241 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
    30301 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
    30361 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
    30421 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
    30481 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
    30541 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
    30601 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
    30661 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
    30721 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
    30781 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
    30841 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
    30901 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
    30961 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
    31021 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
    31081 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
    31141 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
    31201 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
    31261 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
    31321 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
    31381 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
    31441 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
    31501 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
    31561 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
    31621 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
    31681 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
    31741 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
    31801 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
    31861 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
    31921 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
    31981 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
    32041 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
    32101 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
    32161 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
    32221 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
    32281 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
    32341 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
    32401 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
    32461 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
    32521 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
    32581 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
    32641 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
    32701 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
    32761 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
    32821 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
    32881 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
    32941 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
    33001 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
    33061 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
    33121 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
    33181 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
    33241 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
    33301 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
    33361 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
    33421 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
    33481 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
    33541 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
    33601 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
    33661 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
    33721 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
    33781 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
    33841 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
    33901 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
    33961 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
    34021 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
    34081 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
    34141 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
    34201 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
    34261 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
    34321 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
    34381 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
    34441 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
    34501 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
    34561 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
    34621 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
    34681 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
    34741 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
    34801 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
    34861 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
    34921 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
    34981 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
    35041 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
    35101 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
    35161 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
    35221 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
    35281 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
    35341 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
    35401 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
    35461 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
    35521 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
    35581 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
    35641 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    35701 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    35761 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    35821 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    35881 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    35941 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    36001 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    36061 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    36121 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    36181 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    36241 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    36301 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    36361 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    36421 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    36481 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    36541 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    36601 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    36661 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    36721 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    36781 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    36841 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    36901 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    36961 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    37021 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    37081 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    37141 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    37201 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    37261 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    37321 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    37381 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    37441 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    37501 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    37561 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    37621 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    37681 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    37741 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    37801 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    37861 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    37921 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    37981 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    38041 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    38101 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    38161 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    38221 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    38281 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    38341 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    38401 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    38461 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    38521 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    38581 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    38641 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    38701 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    38761 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    38821 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    38881 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    38941 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    39001 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    39061 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    39121 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    39181 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    39241 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    39301 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    39361 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    39421 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    39481 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    39541 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    39601 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    39661 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    39721 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    39781 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    39841 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    39901 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    39961 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    40021 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    40081 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    40141 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    40201 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    40261 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    40321 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    40381 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    40441 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    40501 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    40561 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    40621 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    40681 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    40741 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    40801 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    40861 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    40921 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    40981 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    41041 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    41101 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    41161 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    41221 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    41281 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    41341 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    41401 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    41461 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    41521 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    41581 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    41641 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    41701 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    41761 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    41821 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    41881 tcaatataat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    41941 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    42001 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    42061 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    42121 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    42181 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    42241 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    42301 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    42361 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    42421 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    42481 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    42541 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    42601 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    42661 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    42721 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    42781 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    42841 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    42901 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    42961 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    43021 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    43081 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    43141 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    43201 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    43261 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    43321 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    43381 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    43441 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    43501 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    43561 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    43621 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    43681 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    43741 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    43801 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    43861 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    43921 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    43981 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    44041 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    44101 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    44161 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    44221 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    44281 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    44341 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    44401 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    44461 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    44521 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    44581 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    44641 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    44701 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    44761 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    44821 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    44881 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    44941 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    45001 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    45061 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    45121 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    45181 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    45241 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    45301 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    45361 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    45421 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    45481 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    45541 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    45601 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    45661 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    45721 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    45781 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    45841 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    45901 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    45961 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    46021 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    46081 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    46141 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    46201 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    46261 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    46321 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    46381 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    46441 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    46501 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    46561 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcacgttca ggttttaagg
    46621 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    46681 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    46741 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    46801 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    46861 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    46921 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    46981 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    47041 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    47101 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    47161 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    47221 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    47281 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    47341 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    47401 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    47461 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    47521 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    47581 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    47641 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    47701 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    47761 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    47821 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    47881 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    47941 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    48001 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    48061 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    48121 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    48181 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    48241 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    48301 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    48361 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    48421 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    48481 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    48541 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    48601 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    48661 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    48721 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    48781 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    48841 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    48901 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    48961 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    49021 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    49081 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    49141 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    49201 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    49261 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    49321 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    49381 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    49441 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    49501 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    49561 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    49621 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    49681 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    49741 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    49801 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    49861 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    49921 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    49981 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    50041 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    50101 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    50161 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    50221 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    50281 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    50341 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    50401 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    50461 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    50521 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    50581 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    50641 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    50701 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    50761 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    50821 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    50881 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    50941 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    51001 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    51061 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    51121 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    51181 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    51241 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    51301 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    51361 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    51421 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    51481 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    51541 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    51601 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    51661 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    51721 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    51781 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    51841 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    51901 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    51961 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    52021 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    52081 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    52141 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    52201 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    52261 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    52321 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    52381 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    52441 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    52501 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    52561 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    52621 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    52681 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    52741 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    52801 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    52861 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    52921 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    52981 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    53041 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    53101 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    53161 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    53221 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    53281 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    53341 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    53401 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    53461 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    53521 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    53581 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    53641 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    53701 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    53761 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    53821 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    53881 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    53941 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    54001 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    54061 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    54121 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    54181 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    54241 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    54301 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    54361 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    54421 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    54481 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    54541 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    54601 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    54661 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    54721 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    54781 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    54841 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    54901 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    54961 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    55021 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    55081 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    55141 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    55201 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    55261 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    55321 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    55381 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    55441 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    55501 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    55561 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    55621 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    55681 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    55741 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    55801 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    55861 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    55921 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    55981 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    56041 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    56101 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    56161 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    56221 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    56281 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    56341 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    56401 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    56461 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    56521 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    56581 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    56641 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    56701 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    56761 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    56821 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    56881 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    56941 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    57001 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    57061 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    57121 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    57181 gtacagatac tacctctacc acaagtgata gcacggatac
//
LOCUS       DARDHU010000008        54254 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000008 DARDHU010000000
VERSION     DARDHU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.008"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="SDD03_000534"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="SDD03_000534"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG4303007.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="SDD03_000535"
     CDS             complement(937..1518)
                     /locus_tag="SDD03_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4303008.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="SDD03_000536"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="SDD03_000536"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG4303009.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="SDD03_000537"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="SDD03_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG4303010.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="SDD03_000538"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="SDD03_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG4303011.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="SDD03_000539"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="SDD03_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4303012.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="SDD03_000540"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="SDD03_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG4303013.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="SDD03_000541"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="SDD03_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG4303014.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="SDD03_000542"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="SDD03_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG4303015.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="SDD03_000543"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="SDD03_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG4303016.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="SDD03_000544"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="SDD03_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG4303017.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="SDD03_000545"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="SDD03_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG4303018.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="SDD03_000546"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="SDD03_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG4303019.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="SDD03_000547"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="SDD03_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG4303020.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="SDD03_000548"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="SDD03_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG4303021.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="SDD03_000549"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="SDD03_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG4303022.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="SDD03_000550"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="SDD03_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG4303023.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="SDD03_000551"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="SDD03_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG4303024.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="SDD03_000552"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="SDD03_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG4303025.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="SDD03_000553"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="SDD03_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG4303026.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="SDD03_000554"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="SDD03_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG4303027.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="SDD03_000555"
     CDS             13972..14157
                     /locus_tag="SDD03_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG4303028.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="SDD03_000556"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="SDD03_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG4303029.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="SDD03_000557"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="SDD03_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG4303030.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="SDD03_000558"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="SDD03_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG4303031.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="SDD03_000559"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="SDD03_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG4303032.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="SDD03_000560"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="SDD03_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG4303033.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="SDD03_000561"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="SDD03_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG4303034.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="SDD03_000562"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="SDD03_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG4303035.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="SDD03_000563"
     CDS             18232..18879
                     /locus_tag="SDD03_000563"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG4303036.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="SDD03_000564"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="SDD03_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG4303037.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="SDD03_000565"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="SDD03_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG4303038.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="SDD03_000566"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="SDD03_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG4303039.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="SDD03_000567"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="SDD03_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG4303040.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="SDD03_000568"
     CDS             20317..21255
                     /locus_tag="SDD03_000568"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG4303041.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="SDD03_000569"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="SDD03_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG4303042.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="SDD03_000570"
     CDS             21935..22774
                     /locus_tag="SDD03_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4303043.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="SDD03_000571"
     CDS             22750..23619
                     /locus_tag="SDD03_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4303044.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="SDD03_000572"
     CDS             23609..24406
                     /locus_tag="SDD03_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG4303045.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="SDD03_000573"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="SDD03_000573"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG4303046.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="SDD03_000574"
     CDS             complement(25688..28357)
                     /locus_tag="SDD03_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG4303047.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="SDD03_000575"
     CDS             complement(28595..28783)
                     /locus_tag="SDD03_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG4303048.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="SDD03_000576"
     CDS             29053..29415
                     /locus_tag="SDD03_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4303049.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="SDD03_000577"
     CDS             29467..31149
                     /locus_tag="SDD03_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG4303050.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="SDD03_000578"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="SDD03_000578"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG4303051.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="SDD03_000579"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="SDD03_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG4303052.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="SDD03_000580"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="SDD03_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG4303053.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="SDD03_000581"
     CDS             34984..35988
                     /locus_tag="SDD03_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303054.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="SDD03_000582"
     CDS             35989..36933
                     /locus_tag="SDD03_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303055.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="SDD03_000583"
     CDS             36933..37895
                     /locus_tag="SDD03_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303056.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="SDD03_000584"
     CDS             37922..38836
                     /locus_tag="SDD03_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303057.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="SDD03_000585"
     CDS             38862..40643
                     /locus_tag="SDD03_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4303058.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="SDD03_000586"
     CDS             40991..41677
                     /locus_tag="SDD03_000586"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG4303059.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="SDD03_000587"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="SDD03_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG4303060.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="SDD03_000588"
     CDS             45287..46096
                     /locus_tag="SDD03_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4303061.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="SDD03_000589"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="SDD03_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG4303062.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="SDD03_000590"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="SDD03_000590"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG4303063.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="SDD03_000591"
     CDS             complement(49648..50100)
                     /locus_tag="SDD03_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG4303064.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="SDD03_000592"
     CDS             50250..51200
                     /locus_tag="SDD03_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG4303065.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="SDD03_000593"
     CDS             51193..52152
                     /locus_tag="SDD03_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG4303066.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="SDD03_000594"
     CDS             52149..52904
                     /locus_tag="SDD03_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4303067.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="SDD03_000595"
     CDS             52927..53880
                     /locus_tag="SDD03_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4303068.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        17803 a         9832 c        11685 g        14934 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taag
//
LOCUS       DARDHU010000009        51139 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000009 DARDHU010000000
VERSION     DARDHU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.009"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            330..1175
                     /locus_tag="SDD03_000596"
     CDS             330..1175
                     /locus_tag="SDD03_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4303069.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="SDD03_000597"
     CDS             1456..2676
                     /locus_tag="SDD03_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG4303070.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="SDD03_000598"
     CDS             2673..5393
                     /locus_tag="SDD03_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4303071.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="SDD03_000599"
     CDS             5377..6321
                     /locus_tag="SDD03_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4303072.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="SDD03_000600"
     CDS             complement(6390..7400)
                     /locus_tag="SDD03_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4303073.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="SDD03_000601"
     CDS             complement(7580..7924)
                     /locus_tag="SDD03_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG4303074.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="SDD03_000602"
     CDS             complement(7917..8351)
                     /locus_tag="SDD03_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG4303075.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="SDD03_000603"
     CDS             8489..9226
                     /locus_tag="SDD03_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303076.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="SDD03_000604"
     CDS             9219..10430
                     /locus_tag="SDD03_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303077.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="SDD03_000605"
     CDS             10634..11416
                     /locus_tag="SDD03_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG4303078.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="SDD03_000606"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="SDD03_000606"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG4303079.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="SDD03_000607"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="SDD03_000607"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG4303080.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="SDD03_000608"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="SDD03_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG4303081.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="SDD03_000609"
     CDS             15018..16067
                     /locus_tag="SDD03_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG4303082.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="SDD03_000610"
     CDS             16064..16342
                     /locus_tag="SDD03_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG4303083.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="SDD03_000611"
     CDS             16317..16787
                     /locus_tag="SDD03_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG4303084.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="SDD03_000612"
     CDS             16804..17106
                     /locus_tag="SDD03_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303085.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="SDD03_000613"
     CDS             17123..17494
                     /locus_tag="SDD03_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG4303086.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="SDD03_000614"
     CDS             17466..17825
                     /locus_tag="SDD03_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303087.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="SDD03_000615"
     CDS             17997..19004
                     /locus_tag="SDD03_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4303088.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="SDD03_000616"
     CDS             19029..20213
                     /locus_tag="SDD03_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG4303089.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="SDD03_000617"
     CDS             complement(20650..22233)
                     /locus_tag="SDD03_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4303090.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="SDD03_000618"
     CDS             complement(22811..22954)
                     /locus_tag="SDD03_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG4303091.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="SDD03_000619"
     CDS             complement(23176..23655)
                     /locus_tag="SDD03_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4303092.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="SDD03_000620"
     CDS             complement(23728..24012)
                     /locus_tag="SDD03_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303093.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="SDD03_000621"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SDD03_000621"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="SDD03_000622"
     CDS             complement(24356..25642)
                     /locus_tag="SDD03_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG4303094.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="SDD03_000623"
     CDS             25780..26451
                     /locus_tag="SDD03_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG4303095.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="SDD03_000624"
     CDS             26417..26905
                     /locus_tag="SDD03_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG4303096.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="SDD03_000625"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="SDD03_000625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG4303097.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="SDD03_000626"
     CDS             27866..28630
                     /locus_tag="SDD03_000626"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG4303098.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="SDD03_000627"
     CDS             28863..31076
                     /locus_tag="SDD03_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG4303099.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="SDD03_000628"
     CDS             31087..31533
                     /locus_tag="SDD03_000628"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG4303100.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="SDD03_000629"
     CDS             31704..32963
                     /locus_tag="SDD03_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG4303101.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="SDD03_000630"
     CDS             complement(33052..33999)
                     /locus_tag="SDD03_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4303102.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="SDD03_000631"
     CDS             34393..35685
                     /locus_tag="SDD03_000631"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG4303103.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="SDD03_000632"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="SDD03_000632"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG4303104.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="SDD03_000633"
     CDS             37578..38453
                     /locus_tag="SDD03_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4303105.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="SDD03_000634"
     CDS             38719..40602
                     /locus_tag="SDD03_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG4303106.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="SDD03_000635"
     CDS             complement(40677..40961)
                     /locus_tag="SDD03_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG4303107.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="SDD03_000636"
     CDS             complement(40951..41685)
                     /locus_tag="SDD03_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG4303108.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="SDD03_000637"
     CDS             41814..42437
                     /locus_tag="SDD03_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG4303109.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="SDD03_000638"
     CDS             42629..43750
                     /locus_tag="SDD03_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG4303110.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="SDD03_000639"
     CDS             43757..46885
                     /locus_tag="SDD03_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG4303111.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="SDD03_000640"
     CDS             46904..50098
                     /locus_tag="SDD03_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG4303112.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="SDD03_000641"
     CDS             complement(50244..50951)
                     /locus_tag="SDD03_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4303113.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16737 a         8935 c        10624 g        14843 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgat
//
LOCUS       DARDHU010000010        43685 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000010 DARDHU010000000
VERSION     DARDHU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.010"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(326..2707)
                     /locus_tag="SDD03_000642"
     CDS             complement(326..2707)
                     /locus_tag="SDD03_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG4303114.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2697..3344)
                     /gene="recU"
                     /locus_tag="SDD03_000643"
     CDS             complement(2697..3344)
                     /gene="recU"
                     /locus_tag="SDD03_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG4303115.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3408..3947
                     /locus_tag="SDD03_000644"
     CDS             3408..3947
                     /locus_tag="SDD03_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG4303116.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4037..4456
                     /gene="gpsB"
                     /locus_tag="SDD03_000645"
     CDS             4037..4456
                     /gene="gpsB"
                     /locus_tag="SDD03_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG4303117.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4478..4845
                     /gene="rnpB"
                     /locus_tag="SDD03_000646"
     ncRNA           4478..4845
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SDD03_000646"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5054..6220
                     /locus_tag="SDD03_000647"
     CDS             5054..6220
                     /locus_tag="SDD03_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG4303118.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6266..7762
                     /locus_tag="SDD03_000648"
     CDS             6266..7762
                     /locus_tag="SDD03_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG4303119.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7843..8526
                     /locus_tag="SDD03_000649"
     CDS             7843..8526
                     /locus_tag="SDD03_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4303120.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9171..10361
                     /locus_tag="SDD03_000650"
     CDS             9171..10361
                     /locus_tag="SDD03_000650"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG4303121.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10463..11416
                     /locus_tag="SDD03_000651"
     CDS             10463..11416
                     /locus_tag="SDD03_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG4303122.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11485..12834
                     /locus_tag="SDD03_000652"
     CDS             11485..12834
                     /locus_tag="SDD03_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4303123.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13017..13340)
                     /locus_tag="SDD03_000653"
     CDS             complement(13017..13340)
                     /locus_tag="SDD03_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303124.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13469..14632
                     /locus_tag="SDD03_000654"
     CDS             13469..14632
                     /locus_tag="SDD03_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4303125.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14702..16051
                     /locus_tag="SDD03_000655"
     CDS             14702..16051
                     /locus_tag="SDD03_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4303126.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16096..16527)
                     /locus_tag="SDD03_000656"
     CDS             complement(16096..16527)
                     /locus_tag="SDD03_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4303127.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16915..19557
                     /gene="alaS"
                     /locus_tag="SDD03_000657"
     CDS             16915..19557
                     /gene="alaS"
                     /locus_tag="SDD03_000657"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG4303128.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19757..20353
                     /locus_tag="SDD03_000658"
     CDS             19757..20353
                     /locus_tag="SDD03_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4303129.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20475..21623
                     /locus_tag="SDD03_000659"
     CDS             20475..21623
                     /locus_tag="SDD03_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4303130.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21661..22074)
                     /locus_tag="SDD03_000660"
     CDS             complement(21661..22074)
                     /locus_tag="SDD03_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG4303131.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22153..22584
                     /locus_tag="SDD03_000661"
     CDS             22153..22584
                     /locus_tag="SDD03_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG4303132.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22683..22916)
                     /locus_tag="SDD03_000662"
     CDS             complement(22683..22916)
                     /locus_tag="SDD03_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG4303133.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23099..24769
                     /locus_tag="SDD03_000663"
     CDS             23099..24769
                     /locus_tag="SDD03_000663"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG4303134.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24916..25302
                     /locus_tag="SDD03_000664"
     CDS             24916..25302
                     /locus_tag="SDD03_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303135.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25289..25987
                     /locus_tag="SDD03_000665"
     CDS             25289..25987
                     /locus_tag="SDD03_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4303136.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25992..26456
                     /locus_tag="SDD03_000666"
     CDS             25992..26456
                     /locus_tag="SDD03_000666"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG4303137.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26453..27364
                     /locus_tag="SDD03_000667"
     CDS             26453..27364
                     /locus_tag="SDD03_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4303138.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27599..28138
                     /gene="pyrR"
                     /locus_tag="SDD03_000668"
     CDS             27599..28138
                     /gene="pyrR"
                     /locus_tag="SDD03_000668"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG4303139.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28152..29435
                     /locus_tag="SDD03_000669"
     CDS             28152..29435
                     /locus_tag="SDD03_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG4303140.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29448..30374
                     /locus_tag="SDD03_000670"
     CDS             29448..30374
                     /locus_tag="SDD03_000670"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG4303141.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30393..31673
                     /locus_tag="SDD03_000671"
     CDS             30393..31673
                     /locus_tag="SDD03_000671"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG4303142.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31678..32754
                     /gene="carA"
                     /locus_tag="SDD03_000672"
     CDS             31678..32754
                     /gene="carA"
                     /locus_tag="SDD03_000672"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG4303143.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32759..35944
                     /gene="carB"
                     /locus_tag="SDD03_000673"
     CDS             32759..35944
                     /gene="carB"
                     /locus_tag="SDD03_000673"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG4303144.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35998..36774
                     /locus_tag="SDD03_000674"
     CDS             35998..36774
                     /locus_tag="SDD03_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG4303145.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36771..37703
                     /locus_tag="SDD03_000675"
     CDS             36771..37703
                     /locus_tag="SDD03_000675"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG4303146.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37700..38416
                     /gene="pyrF"
                     /locus_tag="SDD03_000676"
     CDS             37700..38416
                     /gene="pyrF"
                     /locus_tag="SDD03_000676"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG4303147.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38423..39112
                     /locus_tag="SDD03_000677"
     CDS             38423..39112
                     /locus_tag="SDD03_000677"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG4303148.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39085..39354
                     /locus_tag="SDD03_000678"
     CDS             39085..39354
                     /locus_tag="SDD03_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303149.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39392..40033
                     /locus_tag="SDD03_000679"
     CDS             39392..40033
                     /locus_tag="SDD03_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4303150.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40076..40768
                     /locus_tag="SDD03_000680"
     CDS             40076..40768
                     /locus_tag="SDD03_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG4303151.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40765..41667
                     /locus_tag="SDD03_000681"
     CDS             40765..41667
                     /locus_tag="SDD03_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4303152.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41825..43531)
                     /gene="efbA"
                     /locus_tag="SDD03_000682"
     CDS             complement(41825..43531)
                     /gene="efbA"
                     /locus_tag="SDD03_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG4303153.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13996 a         7698 c         9488 g        12503 t
ORIGIN      
        1 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
       61 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
      121 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
      181 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
      241 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
      301 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
      361 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
      421 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
      481 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
      541 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
      601 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
      661 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
      721 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
      781 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
      841 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
      901 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
      961 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     1021 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
     1081 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
     1141 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
     1201 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
     1261 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
     1321 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
     1381 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
     1441 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
     1501 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
     1561 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
     1621 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
     1681 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
     1741 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
     1801 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
     1861 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
     1921 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
     1981 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
     2041 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
     2101 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
     2161 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
     2221 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
     2281 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
     2341 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
     2401 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
     2461 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
     2521 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
     2581 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
     2641 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
     2701 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
     2761 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
     2821 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
     2881 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
     2941 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
     3001 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
     3061 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
     3121 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
     3181 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
     3241 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
     3301 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
     3361 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
     3421 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
     3481 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
     3541 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
     3601 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
     3661 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
     3721 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
     3781 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
     3841 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
     3901 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
     3961 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
     4021 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
     4081 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
     4141 catcaaagat tatgaagctt acagcaaaga actccttgct ttacaagaag aaaacgatcg
     4201 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
     4261 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
     4321 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
     4381 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
     4441 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
     4501 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
     4561 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
     4621 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
     4681 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
     4741 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
     4801 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
     4861 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
     4921 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
     4981 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
     5041 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
     5101 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
     5161 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
     5221 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
     5281 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
     5341 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
     5401 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
     5461 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
     5521 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
     5581 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
     5641 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
     5701 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
     5761 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
     5821 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
     5881 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
     5941 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
     6001 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
     6061 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
     6121 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
     6181 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
     6241 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
     6301 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
     6361 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
     6421 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
     6481 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
     6541 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
     6601 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
     6661 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
     6721 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
     6781 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
     6841 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
     6901 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
     6961 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
     7021 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
     7081 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
     7141 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
     7201 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
     7261 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
     7321 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
     7381 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
     7441 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
     7501 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
     7561 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
     7621 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
     7681 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
     7741 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
     7801 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
     7861 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
     7921 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
     7981 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
     8041 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
     8101 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
     8161 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
     8221 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
     8281 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
     8341 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
     8401 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
     8461 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
     8521 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
     8581 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
     8641 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
     8701 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
     8761 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
     8821 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
     8881 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
     8941 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
     9001 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
     9061 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
     9121 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
     9181 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
     9241 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
     9301 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
     9361 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
     9421 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
     9481 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
     9541 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
     9601 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
     9661 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
     9721 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
     9781 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
     9841 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
     9901 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
     9961 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    10021 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    10081 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    10141 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    10201 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    10261 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    10321 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    10381 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    10441 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    10501 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    10561 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    10621 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    10681 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    10741 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    10801 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    10861 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    10921 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    10981 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    11041 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    11101 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    11161 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    11221 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    11281 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    11341 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    11401 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    11461 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    11521 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    11581 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    11641 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    11701 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    11761 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    11821 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    11881 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    11941 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    12001 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    12061 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    12121 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    12181 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    12241 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    12301 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    12361 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    12421 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    12481 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    12541 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    12601 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    12661 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    12721 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    12781 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    12841 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    12901 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    12961 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    13021 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    13081 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    13141 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    13201 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    13261 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    13321 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    13381 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    13441 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    13501 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    13561 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    13621 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    13681 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    13741 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    13801 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    13861 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    13921 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    13981 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    14041 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    14101 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    14161 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    14221 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    14281 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    14341 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    14401 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    14461 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    14521 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    14581 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    14641 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    14701 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    14761 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    14821 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    14881 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    14941 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    15001 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    15061 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    15121 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    15181 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    15241 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    15301 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    15361 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    15421 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    15481 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    15541 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    15601 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    15661 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    15721 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    15781 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    15841 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    15901 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    15961 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    16021 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    16081 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    16141 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    16201 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    16261 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    16321 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    16381 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    16441 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    16501 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    16561 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    16621 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaattaa gtacgaaaag
    16681 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    16741 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    16801 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    16861 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    16921 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    16981 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    17041 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    17101 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    17161 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    17221 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    17281 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    17341 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    17401 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    17461 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    17521 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    17581 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    17641 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    17701 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    17761 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    17821 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    17881 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    17941 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    18001 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    18061 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    18121 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    18181 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    18241 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    18301 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    18361 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    18421 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    18481 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    18541 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    18601 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    18661 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    18721 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    18781 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    18841 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    18901 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    18961 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    19021 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    19081 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    19141 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    19201 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    19261 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    19321 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    19381 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    19441 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    19501 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    19561 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    19621 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    19681 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    19741 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    19801 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    19861 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    19921 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    19981 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    20041 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    20101 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    20161 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    20221 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    20281 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    20341 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    20401 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    20461 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    20521 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    20581 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    20641 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    20701 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    20761 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    20821 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    20881 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    20941 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    21001 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    21061 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    21121 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    21181 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    21241 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    21301 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    21361 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    21421 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    21481 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    21541 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    21601 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    21661 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    21721 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    21781 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    21841 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    21901 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    21961 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    22021 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    22081 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    22141 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    22201 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    22261 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    22321 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    22381 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    22441 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    22501 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    22561 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    22621 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    22681 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    22741 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    22801 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    22861 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    22921 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    22981 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    23041 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    23101 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    23161 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    23221 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    23281 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    23341 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    23401 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    23461 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    23521 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    23581 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    23641 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    23701 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    23761 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    23821 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    23881 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    23941 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    24001 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    24061 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    24121 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    24181 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    24241 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    24301 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    24361 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    24421 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    24481 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    24541 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    24601 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    24661 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    24721 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    24781 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    24841 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    24901 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    24961 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    25021 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    25081 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    25141 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    25201 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    25261 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    25321 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    25381 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    25441 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    25501 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    25561 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    25621 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    25681 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    25741 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    25801 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    25861 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    25921 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    25981 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    26041 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    26101 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    26161 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    26221 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    26281 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    26341 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    26401 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    26461 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    26521 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    26581 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    26641 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    26701 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    26761 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    26821 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    26881 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    26941 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    27001 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    27061 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    27121 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    27181 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    27241 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    27301 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    27361 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    27421 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    27481 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    27541 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    27601 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    27661 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    27721 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    27781 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    27841 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    27901 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    27961 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    28021 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    28081 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    28141 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    28201 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    28261 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    28321 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    28381 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    28441 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    28501 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    28561 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    28621 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    28681 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    28741 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    28801 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    28861 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    28921 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    28981 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    29041 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    29101 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    29161 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    29221 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    29281 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    29341 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    29401 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    29461 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    29521 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    29581 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    29641 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    29701 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    29761 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    29821 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    29881 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    29941 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    30001 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    30061 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    30121 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    30181 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    30241 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    30301 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    30361 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    30421 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    30481 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    30541 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    30601 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    30661 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    30721 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    30781 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    30841 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    30901 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    30961 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    31021 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    31081 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    31141 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    31201 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    31261 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    31321 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    31381 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    31441 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    31501 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    31561 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    31621 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    31681 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    31741 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    31801 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    31861 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    31921 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    31981 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    32041 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    32101 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    32161 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    32221 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    32281 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    32341 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    32401 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    32461 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    32521 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    32581 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    32641 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    32701 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    32761 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    32821 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    32881 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    32941 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    33001 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    33061 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    33121 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    33181 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    33241 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    33301 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    33361 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    33421 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    33481 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    33541 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    33601 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    33661 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    33721 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    33781 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    33841 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    33901 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    33961 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    34021 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    34081 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    34141 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    34201 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    34261 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    34321 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    34381 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    34441 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    34501 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    34561 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    34621 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    34681 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    34741 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    34801 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    34861 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    34921 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    34981 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    35041 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    35101 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    35161 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    35221 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    35281 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    35341 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    35401 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    35461 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    35521 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    35581 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    35641 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    35701 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    35761 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    35821 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    35881 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    35941 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    36001 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    36061 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    36121 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    36181 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    36241 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    36301 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    36361 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    36421 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    36481 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    36541 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    36601 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    36661 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    36721 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    36781 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    36841 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    36901 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    36961 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    37021 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    37081 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    37141 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    37201 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    37261 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    37321 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    37381 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    37441 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    37501 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    37561 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    37621 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    37681 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    37741 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    37801 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    37861 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    37921 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    37981 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    38041 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    38101 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    38161 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    38221 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    38281 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    38341 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    38401 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    38461 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    38521 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    38581 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    38641 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    38701 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    38761 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    38821 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    38881 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    38941 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    39001 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattg aagcgcgctt
    39061 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    39121 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    39181 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    39241 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    39301 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    39361 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    39421 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    39481 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    39541 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    39601 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    39661 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    39721 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    39781 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    39841 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    39901 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    39961 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    40021 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    40081 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    40141 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    40201 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    40261 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    40321 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    40381 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    40441 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    40501 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    40561 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    40621 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    40681 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    40741 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    40801 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    40861 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    40921 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    40981 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    41041 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    41101 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    41161 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    41221 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    41281 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    41341 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    41401 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    41461 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    41521 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    41581 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    41641 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    41701 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    41761 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    41821 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    41881 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    41941 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    42001 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    42061 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    42121 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    42181 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    42241 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    42301 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    42361 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    42421 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    42481 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    42541 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    42601 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    42661 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    42721 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    42781 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    42841 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    42901 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    42961 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    43021 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    43081 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    43141 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    43201 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    43261 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    43321 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    43381 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    43441 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    43501 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    43561 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    43621 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    43681 ttagg
//
LOCUS       DARDHU010000011        42551 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000011 DARDHU010000000
VERSION     DARDHU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.011"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            831..2441
                     /locus_tag="SDD03_000683"
     CDS             831..2441
                     /locus_tag="SDD03_000683"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG4303154.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2733..3602
                     /locus_tag="SDD03_000684"
     CDS             2733..3602
                     /locus_tag="SDD03_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG4303155.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3767..5038
                     /locus_tag="SDD03_000685"
     CDS             3767..5038
                     /locus_tag="SDD03_000685"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG4303156.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            5035..6330
                     /gene="rho"
                     /locus_tag="SDD03_000686"
     CDS             5035..6330
                     /gene="rho"
                     /locus_tag="SDD03_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG4303157.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6429..6692
                     /locus_tag="SDD03_000687"
     CDS             6429..6692
                     /locus_tag="SDD03_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG4303158.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            7137..7586
                     /locus_tag="SDD03_000688"
     CDS             7137..7586
                     /locus_tag="SDD03_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4303159.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7643..9061
                     /locus_tag="SDD03_000689"
     CDS             7643..9061
                     /locus_tag="SDD03_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4303160.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            9061..9921
                     /locus_tag="SDD03_000690"
     CDS             9061..9921
                     /locus_tag="SDD03_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG4303161.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            10095..10868
                     /locus_tag="SDD03_000691"
     CDS             10095..10868
                     /locus_tag="SDD03_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4303162.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            11015..11380
                     /locus_tag="SDD03_000692"
     CDS             11015..11380
                     /locus_tag="SDD03_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG4303163.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11528..12736)
                     /locus_tag="SDD03_000693"
     CDS             complement(11528..12736)
                     /locus_tag="SDD03_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4303164.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12755..13033)
                     /locus_tag="SDD03_000694"
     CDS             complement(12755..13033)
                     /locus_tag="SDD03_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303165.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13187..13882
                     /locus_tag="SDD03_000695"
     CDS             13187..13882
                     /locus_tag="SDD03_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4303166.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(14324..15121)
                     /locus_tag="SDD03_000696"
     CDS             complement(14324..15121)
                     /locus_tag="SDD03_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG4303167.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(15118..16302)
                     /locus_tag="SDD03_000697"
     CDS             complement(15118..16302)
                     /locus_tag="SDD03_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG4303168.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16327..17574)
                     /locus_tag="SDD03_000698"
     CDS             complement(16327..17574)
                     /locus_tag="SDD03_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG4303169.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17789..19534
                     /gene="dnaX"
                     /locus_tag="SDD03_000699"
     CDS             17789..19534
                     /gene="dnaX"
                     /locus_tag="SDD03_000699"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG4303170.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19554..19868
                     /locus_tag="SDD03_000700"
     CDS             19554..19868
                     /locus_tag="SDD03_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG4303171.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19893..20489
                     /gene="recR"
                     /locus_tag="SDD03_000701"
     CDS             19893..20489
                     /gene="recR"
                     /locus_tag="SDD03_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG4303172.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20553..21197
                     /locus_tag="SDD03_000702"
     CDS             20553..21197
                     /locus_tag="SDD03_000702"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG4303173.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21212..21541
                     /locus_tag="SDD03_000703"
     CDS             21212..21541
                     /locus_tag="SDD03_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG4303174.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21555..22493
                     /gene="holB"
                     /locus_tag="SDD03_000704"
     CDS             21555..22493
                     /gene="holB"
                     /locus_tag="SDD03_000704"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG4303175.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22529..23353
                     /locus_tag="SDD03_000705"
     CDS             22529..23353
                     /locus_tag="SDD03_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG4303176.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23346..23693
                     /locus_tag="SDD03_000706"
     CDS             23346..23693
                     /locus_tag="SDD03_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG4303177.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23762..24634
                     /gene="rsmI"
                     /locus_tag="SDD03_000707"
     CDS             23762..24634
                     /gene="rsmI"
                     /locus_tag="SDD03_000707"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG4303178.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24743..25864
                     /gene="dinB"
                     /locus_tag="SDD03_000708"
     CDS             24743..25864
                     /gene="dinB"
                     /locus_tag="SDD03_000708"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG4303179.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25918..26520)
                     /gene="nrdG"
                     /locus_tag="SDD03_000709"
     CDS             complement(25918..26520)
                     /gene="nrdG"
                     /locus_tag="SDD03_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG4303180.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26835..29024)
                     /gene="nrdD"
                     /locus_tag="SDD03_000710"
     CDS             complement(26835..29024)
                     /gene="nrdD"
                     /locus_tag="SDD03_000710"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG4303181.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29323..30117
                     /locus_tag="SDD03_000711"
     CDS             29323..30117
                     /locus_tag="SDD03_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303182.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            30107..32149
                     /locus_tag="SDD03_000712"
     CDS             30107..32149
                     /locus_tag="SDD03_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303183.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32430..32579
                     /locus_tag="SDD03_000713"
     CDS             32430..32579
                     /locus_tag="SDD03_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG4303184.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32590..34104
                     /gene="dltA"
                     /locus_tag="SDD03_000714"
     CDS             32590..34104
                     /gene="dltA"
                     /locus_tag="SDD03_000714"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG4303185.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            34101..35306
                     /gene="dltB"
                     /locus_tag="SDD03_000715"
     CDS             34101..35306
                     /gene="dltB"
                     /locus_tag="SDD03_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG4303186.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35351..35584
                     /gene="dltC"
                     /locus_tag="SDD03_000716"
     CDS             35351..35584
                     /gene="dltC"
                     /locus_tag="SDD03_000716"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG4303187.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35587..36855
                     /gene="dltD"
                     /locus_tag="SDD03_000717"
     CDS             35587..36855
                     /gene="dltD"
                     /locus_tag="SDD03_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG4303188.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36996..38078
                     /locus_tag="SDD03_000718"
     CDS             36996..38078
                     /locus_tag="SDD03_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG4303189.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            38078..38881
                     /locus_tag="SDD03_000719"
     CDS             38078..38881
                     /locus_tag="SDD03_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG4303190.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38951..39691
                     /locus_tag="SDD03_000720"
     CDS             38951..39691
                     /locus_tag="SDD03_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4303191.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39702..40706
                     /locus_tag="SDD03_000721"
     CDS             39702..40706
                     /locus_tag="SDD03_000721"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG4303192.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40751..42514)
                     /locus_tag="SDD03_000722"
     CDS             complement(40751..42514)
                     /locus_tag="SDD03_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303193.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        13669 a         7443 c         8833 g        12606 t
ORIGIN      
        1 ctggcttttg aataccgttt atccagaggt tgtgagccta ccatttaact ccgaatcaca
       61 agaaataatt tcaaaaacag cttctcatgt ttgatggata ttgtgacttg tgaccgagga
      121 gttggcagga gaacaccgtt tatttagaaa taaggaggca cgacttttgt cgcatctcct
      181 tattttttac ttatgtgatt cgttaccttc aatgaatccc tacaaggcac ttttgttgaa
      241 aaaaaggtca tctttgaaaa aattagtgga attgttttgt ttttgatatt atttaataga
      301 aaagatatgc caaaaatccg agaactgtgg actacgtttt ttacgtagag gaggtaaata
      361 agggtgaaag aaaaagattg tttctacgat aagctatata aactgttgct gcaagtagga
      421 aaaagtagtt ctttgttagg tgggatatta tctatcataa tgtttgtttt atttttttat
      481 ctattatttc acttaaaatt tttcagtaac gattttctga tttgcttagg tttaacgtta
      541 gctatgttta tatcaagctt attatgtggg ttaaattttt tttgcactca taaaggactt
      601 aagaaataat ttctcaaagg atatcgaaaa agaaaaaatt gtaagcggat agtattcatt
      661 tgagtacttg actttccctt agtcagcttg tattatacac cttgggcacc cttttagagg
      721 gtcagaagtt tacagaaatt tcggctccct attcttattg aagaataggg agctttgttt
      781 attgtctgag acttttcctg cacataaatt aaaaaggagt ttttgaactt atgacaaaat
      841 atatttttgt gaccggcggt gtcgtttcat caatcggaaa agggattgtt gcagcttctt
      901 taggacgctt attgaaaaac cgcggcttaa aagtaactat ccaaaaattt gatccataca
      961 tcaacgtgga tccggggacg atgagcccat atcagcacgg agaagtattc gtaacagatg
     1021 atggtgctga aacagatttg gatctgggcc actacgaacg ttttattgac atcaatttga
     1081 acaaatactc caacgtgacg acaggaaaaa tctattctga agttttgcgt aaagaacgta
     1141 agggcgaata cttgggagca actgttcaag ttattcctca tattacaaat gagatcaaag
     1201 aaaaaatcat gcgagcagct aaaatgactg attcagatgt gatcattaca gaagtagggg
     1261 gaacagtagg ggatatagaa tctttgccat tccttgaagc acttcgtcaa atgaaagcag
     1321 atgtcggtac agataatgtt atgtatatcc atacaacatt gattccttac ttgaaagctg
     1381 ctggagaaat gaaaactaaa ccaacgcagc acagtgtaaa agagctacgt ggtttaggta
     1441 tccaaccgaa tattttggtg gtacgtacag agcaacctgt ttctcaaagc gtgaaaaaca
     1501 aattggcaca attttgtgat gtcgaaccag aagcagttat cgagtcgcca gatgtagata
     1561 cactttactc catcccatta gttttacaag cacaaggaat ggatcaaatt gtttgcgacc
     1621 atttaaaact agatacacca gaagcagaca tgacagaatg gaaagaatta gaagaacgtg
     1681 tcttgaactt gaagaaaaaa gtacggattg cgcttgtcgg taaatatgtc gagttaccag
     1741 atgcatatat ctcagtagtt gaagcattga aacattctgg tttcgcctat aattcagaca
     1801 tcgagatcga ttggatcaaa gcacaagaat taacgagaga aaatgtagaa gaacgtttga
     1861 aagacgcaga tggaatcttg gttcctggtg gatttggtga tcgcggagta gaagggaaaa
     1921 tcgaagcgat tcgttatgct cgcgaaaatg acgttccgtt tttaggtatt tgtcttggta
     1981 tgcagatggc ttgtgtcgaa tttgcacgta acgtcgttgg attagaagat gcgtcatcag
     2041 ctgaaacgaa tccagataca gcaaacaata ttattgattt aatggctgat caggaaaaca
     2101 tcgagaattt gggtggaact ttacgccttg gattataccc atgtaaaatc aaaaaaggaa
     2161 cgaagacggc tgaagcatat gatggagccg atgttgtgca agaacgtcac cgtcaccgct
     2221 atgagttcaa taacaaatat cgtcaattgt ttgaagaaca agggctagtc ttttcaggtg
     2281 tctcaccaga taaccgttta gtagaaatcg tagagctgcc agagaaaaaa ttctttgtgg
     2341 gatgccaatt ccacccagaa ttgatttctc gtccaaatcg cccgcaaaaa ttaatcaaag
     2401 catttgtcgg agcatcatta gcagatcacg aaacaaaata atttttgaac ttgatagaaa
     2461 gagaaaagta gcaagagaag gggataagca attttcttgt cacttttctc tttttctgat
     2521 ttgttattat ttgtaggaaa aaagatagaa agcaaaagac ttttttcgat taaagcgcta
     2581 ttggatattt tattttttgc aaaaaacttc ttgaaaattt tcattttcat tgattttagt
     2641 agaaaactac cgaaatgttt ggtaaaatag ggtcgttggt taagaattgt cttaacaaat
     2701 ttagttttac aaaacttagg aggaatttta ttatgccagt agtatcagga gctgaatttt
     2761 taaaagctgc tcgtaaaggc ggttacgctg tcggcggatt caacacaaac aacttggaat
     2821 ggactcaagc aatccttgaa gcggctgaag caaaacaagc accagtactt atccaaacat
     2881 caatgggtgc agctaaatac atgggtggtt acaaaatctg taaagattta atcactgatt
     2941 tagttgaagc aatgaacatc actgttccag ttgctatcca cttagaccat ggtgattacg
     3001 aagctgcttt ggaatgtatc gaattaggct atacttcagt aatgttcgat ggttcacatc
     3061 taccatttga agaaaacttg aaattagcta aagacgttgt tgaaaaagca catgcaaaag
     3121 gtatctcagt tgaatgtgaa gttggttcaa tcggcggaga agaagacgga atcatcggta
     3181 acggcgaatt agctgatatt gaagaatgca aacaaatggt tgctacaggt atcgactact
     3241 tagcttgcgg tatcggtaac atccatggtc aatatccaga aaactggaaa ggattagctt
     3301 ttgaccactt acaagcaatt gctgatgctg taggtccaga catgccttta gtattacatg
     3361 gcggatcagg tattcctcaa gatcaaatcg aaaaagctat ctcaatgggt atctcaaaag
     3421 taaatgttaa cactgaattc caattatcat ttgctgcagc tactcgtaaa tatatcgaag
     3481 ctggtaaaga taaagaaggt aaaggattcg accctcgtaa attattagct ccaggtaaag
     3541 cagcaatcgt tgaagatgct aaatctcata tcgactggtt cggttcagct aacaaagcgt
     3601 aagcatccac ataacagata cgatatatag gaatggagaa cgacttttga agtcgctctc
     3661 cattcttttt tacttaaaac ggcagaccat gctcgtttct tttttttatc tatgataaaa
     3721 tacaagcata tccatgaaaa aagattaata aacaaggtgg aaacagatga aaaaaatcgt
     3781 aatcaaagga aaccgacctt tagctggtga agtaacgatc agcggtgcaa aaaatagtgc
     3841 agtcgcattg attccagctg caatcttagc tgattcccca gtgatcttgg aaggtgtgcc
     3901 ggacatacaa gatgttcatt cattaattga aattttagag atcatgggcg caactgtcca
     3961 tttttccaac aacatactag aaatcgatcc tcgagaagtc gtatcgattc cgatgccgca
     4021 cggaaaaatc aatagtttgc gtgcttctta ttattttatg ggaacactgc ttggtaaatt
     4081 tggcgaagcg gtagtaggcc tgccaggtgg ctgttattta ggccctagac cgattgacct
     4141 acacgtcaaa ggatttgaag ctctaggtgc tgaagtgaca aatgaacatg gtgccatgta
     4201 tttacggaca gaaaataaag gattgcgtgg caatcggatt ttcatggacg tggtttcagt
     4261 cggagcaacg atcaatgtga tgttagctgc tgtaaaagca aaaggacaga cagtaattga
     4321 aaacgctgca cgagaaccgg aaatcataga tgtggcaact ttactgaata atatgggggc
     4381 aaaagttcga ggagcgggta cagatgtgat ccgtatcgaa ggagtcgaaa cattacatgg
     4441 ctgtcggcat ttcatgattc ctgaccgcat cgaagcagga acctatctag cattagcagc
     4501 tgcagtagga aatggtatca aagtgaaaaa tgtgatcttt gaacatttgg aaagctttat
     4561 agcaaaattg caggaaattg gtgtccatat gaaaatcagc gaagatgaaa tcgaagttta
     4621 cccctcgaaa gaattaaaac ccgcaaacat catgacctat ccttatccag gatttgccac
     4681 agatctgcag cagccactta ctgcgttact attgatgact acaggcactt cagaaatcat
     4741 cgatacgatc tatgcgaaac gtgtgaacca tgtgccggaa ctagcacgaa tgggcgctga
     4801 tattaccgtg gaaggaaata tgatcatcgt gaatggtcca aataaacttc acggcacaga
     4861 agtcgtggct tctgatcttc gggcaggtgc ttgtttagtg attgctggac tattggcaga
     4921 agggacaacg acgatttaca atgtagatta tatcttacgc ggttatgatc atattatcga
     4981 gaaactaaca gccttagggg catcaattga aatgatagag gaagtgggag ctgaatgagc
     5041 gattatttga caatggcaga actagagaac aaaacgttaa aagaagttta tagttatgcc
     5101 aaagaattta aaattcctta ttacagtaag atgaataaaa aggaattatc tctagctgtg
     5161 attcgtgcac aagcagagaa acaaggattt tattatatgg aaggaatatt ggatatcgtt
     5221 ggtcaagatg gttacggttt tttacggcca atcaattatg gaccaagtgc agaagatatc
     5281 tatatttctg cctcacaaat cagacgattc agtttgagaa atggggacaa agtggcagga
     5341 aaagcccgtc cacctaaaga atcggaacgt tattatggtt tgatgcacgt ggaaagcgtt
     5401 aatggaaaag atccggaaga agcaaaagaa cgtccgcatt ttccagcctt aacacctctt
     5461 tatccgcaaa gacagcttca actggaaaca acaccgcaaa gactatctac ccgaatgatc
     5521 gatatctttt ctccaatcgg ttttggtcag cgtggattga tcgtcgcacc gccaaaagca
     5581 gggaaaactt ctgtattaaa agaaatcgca aatggtatca ccgaaaacta tccggaagta
     5641 gaattaatcg ttctactgat tgatgaacga ccggaagaag taacggatct agaacgaagc
     5701 gtgaaaggcg acgtggtatc ttctacattt gatctgcagc cgcaaaacca tactcgagtt
     5761 tctgaattag tattggaacg cgcaatgcgt ttagtcgaag ataaacgaga tgtcgttatc
     5821 ttgatggata gtattactag acttgctcgg gcctataact tagtcgttcc accaagcgga
     5881 cgtacattga gtggcgggat tgacccagcc gcattgtata aacctaagaa atttttcggg
     5941 gcagcaagaa acatcgaaga aggtggcagt ttgacgattt tagcgaccgc tttagtcgat
     6001 acaggaagcc gtatggatga cgtgatctat gaagaattca aaggaactgg aaatctagaa
     6061 ttacaacttt cacgtgaatt atctgaacgt cggatcttcc cagccatcga tatcaaaaaa
     6121 tcaggtaccc gaaaagaaga attattgatg ccaagtgaag aattagaaga aatttggaaa
     6181 cttcgaaaaa atatgactgg agattcatta gaatatacgg aacaactgat ccgctttttg
     6241 cgtaaaacaa aaaacaatca gcaatttttc aaagaatttc aagatgtctc ctttggtaaa
     6301 aaaaatccga ccagaaatct gaaaagataa ttgcaacctt gactgaaaca tgttaagatg
     6361 ttaatgttgt aaatcgacaa atcaactctg gttcagcaaa tggagcaggg caaaggagcg
     6421 aaaaaatcat gaaagaaaac atccatccag attaccaccc agtagtattc atggattcaa
     6481 caactggttt caaattcttg tcaggttcaa caaaaacttc acaagaaaca gttgaatggg
     6541 aagacggtaa tacataccca gtcatccgtg tcgaagtaac ttctgactca catccattct
     6601 acactggacg tcaaaaattc acacaagcag acggacgtgt ggaccgtttc aacaaaaaat
     6661 acggtctcaa agacgcaaac gctgcggaat aataaaaacg ctgataactc aacgtttagc
     6721 agactatctg atagggatag tctgtttttt tatgtttttt gccaatttaa aacttcgttt
     6781 gatgctgaaa aagtcgtagg atcataatgc acaaaacaat gaaaaactag tatatagttt
     6841 taattatcca cttgataaaa acaccttata ttaacgaaat gaagaggggt tatttcgaag
     6901 taaaaaacca tttacctaaa attagaagct aagctttcct ttcaaggaag gctttttgtt
     6961 ttgttataga cttgcgattg tttgtgagct agatcgttca ttttaatgac ttatacacgc
     7021 ggatgatggg tctttttcct ctgttcattc ataatattga acggtacagc tagcgatgct
     7081 atactgacct cagttccaag aaaacgttaa cattttttgt gggggagagg ttttttatga
     7141 ggtttgatca attgatcgca gaagaactga ttgttttcga tccatcgata gattcaaaag
     7201 gatcattatt tgagcgaata ggggatgtgt tgaaaaaaac aaataaggta agaaatgttc
     7261 aaaaagtcgt taaggcacta ttcaaacgag aggaggagat ctccacagga atagaagatg
     7321 gatttgggat tccccatgca aaaagcaaat atgtgacaaa accagcactg tgtttttttc
     7381 atacagccaa attaacggac tatagcggga tagatgaaat gccaattgaa tatatttttg
     7441 ccatcgtagt tcctgaaaaa tcagcagata tgcatctaga gattctcagc acactctcca
     7501 gacgattgat gaatgcagat tttagaaagg gaataaaaga agccactacg ccagcagata
     7561 tcatcagaca gattagtcaa gaatgaaaat agtagagtct ttgtccgagt atcgatagtg
     7621 aagaaaaagg aggaaaataa tcatgaaaat cgttggtgta actgcttgcc cgactggaat
     7681 tgctcatact tatatgtcgg cagaaaaatt agaagtaaca gcaagaaatc taaaccatca
     7741 agcaaaattc gaaacacaag gtgtaaaaac agaaaatcag ttatctgaac aagaaatcaa
     7801 agaggcagat gcaatcattc ttgcagtaga taaagaaatc gaattggacc gatttgccgg
     7861 gaaaaaagta aagcgtgtct ctacaagccg agcaatcaaa gaaccgcaag tagtaataga
     7921 cgaagcattg agagatattg gcgtattcgt tgtgtctaat gaaaaagaac cagctgcaaa
     7981 tgagaaaaag ccgacaatct ataatcattt catgaatggt gtgaattaca tgcttccatt
     8041 tgtgatcgca ggcggaatca tcatcgcact gagttttgcc tttgggatta cagcggctga
     8101 tcccaactca gcagactata atgtgttagc agctgcattt tcaaggatcg gaggagatac
     8161 agcgtttgcc atgatggttc ctgcgttagg cgcggggatt gctacatcga ttgctggaaa
     8221 agcaggtttt gccccaggaa ttgttgccgg attactggcc tctacaggtg gttcaggttt
     8281 tcttggcgga atgattggtg gcgtgctagc agggtatatc tgtgattttc tggcaaataa
     8341 aattagcgta aaaaaagaat tttctgctat ttatcagctg attgtcgtcc cctttttatc
     8401 gatcttaatt attgggcttt tgatggtatt tgtgattgaa caaccgatca catgggtgtt
     8461 ggatgctttg acaaattggc tgaatagctt gggaaataca tccgggctat tgttcggatt
     8521 acttatcggg atcatgatgg cagctgatat gggtgggccg atcaataaat cgacttcgac
     8581 tttctcgatc ggtttgatgt cagctggagt aaatgcaccg attgctgctt gtatggctgc
     8641 aggaatgaca cctcccctag gtattgcttt agccacttta ttattcaaac ataaatttac
     8701 caaagaagaa cggacttcag gtcaatcatg ctggatttta ggcgcttcgt atattacaga
     8761 aggtgctata ccgtttgcgg tatctgaccc gatccgcgtg atacctagtt tgatgacagg
     8821 ttcagcagtc gctgcaggga tttccatggc agtaggagtt acttcaatgg cacctcatgg
     8881 agggatttgg gtgatgctaa ttccaaatgt tatgaacaat ttacctatgt atgtcttggc
     8941 aattgcatca ggtacagtca ctacagcctt tatgcttgga ctattgaaaa aaacagtgaa
     9001 agagaaaaaa caagacaagt ggacgaaaaa tgaaaatcta acaaatgagg tggaagcgta
     9061 atgttatata caatgaaaga gttattgaaa gtggctaaag agaatcaatt tgcagtacca
     9121 gccttcaata tttgcagcta tgatatgatg aaagcaatca tcgaagaagc agagagatta
     9181 gaagcaccag taatcttaga aattcatcct gatgaaatcg cctatttaag tgatgagttt
     9241 gttgcggcag taagagccta tgcttataca agtaaactgc cgatcgttat ccacttggat
     9301 catggcggta cgatagaaga tgtcttacgg gcgattcgaa acggctattc ttccgtgatg
     9361 atcgatgcgt ctttgaaatc attcgacgac aatgtacaga ttacgaaaaa agtggttgcg
     9421 ttagctcatg cggtaaatgt ctcagtggag gctgagcttg gaacgattgg gaataatggt
     9481 tctgctgaag gaggtgctga aacaatcatt tatacagatc ctgatcaagc gaagattttt
     9541 gtgaaagaaa caggaatcga tacacttgct gtagcaattg gaacagcgca tggcttgtat
     9601 ccgaaaaaca gaactccaaa attaaatatc gacttactga aagaattgaa ccaacaaatc
     9661 gatcttccat ttgttttaca tggcggttca ggtaatccgg acgcagaagt cagcgaagca
     9721 gtaaaatacg gagttgccaa agtgaatttg agctcagatc ttaaaagcgt ctttttcaat
     9781 gctttgagag aaatattgag tgaaaatcca tcgatgtacg agccgaatgc aatttatcca
     9841 gcagctaata aaaaggtaca agaagtggtc aaacataaaa tggaaatttt gaacacagta
     9901 ggcaaggcta gtttatacta aaaataaaaa tgtgtggtca agagggtatg acaatagtct
     9961 tgtcataccc tcttaaccac acacgatgtt cagaagtagt tttttcgaaa aatttcttct
    10021 tatttctcga agccaatcac ttctgtcaca acctcttttt tcctgttact atataaagta
    10081 aatggggtga ttttatgttg aatgaagtgg agaatagaca gcaggagatt gtgaattatc
    10141 taaaaaacaa ccaatttgca agagtcaccg atttgatcga gttagtaaat tatagcgaag
    10201 ccacgattaa acgtgacctt gtttttctgg aaaaaagtgg attgatccgt agagttcgcg
    10261 gtggcgccat gcttattgac aatcgaaaaa ttgacgtgcc ttacctgatg aagatcacca
    10321 aattagaaga agaaacagaa aaacgtaaga ttgcagatac agcttcttct ttgattcgag
    10381 atgatatggt cttattcttg gattccagta caacctcttt acacttagtc agaaacctag
    10441 caagatttga tggtttgcag atcattacga atggcatcgt cacagctgct atgttgagtg
    10501 aatatacaac ggcaaaagta tctgtattgg gaggggcaat tgttccgaaa cgagcaacaa
    10561 tcaacggttc aaaagcctat aacgatgcat tgtcatatta tgcggattta gcgataatga
    10621 gttgtcgcgg gctagatttc agccaaggag taacagaaac tcatgaagga gaggcaatgg
    10681 tcaaaaaagc ttttagaaaa caatcagacc ggcttgccgt tttagttaca cagcaaaaaa
    10741 ttgcacataa attcatgcat caagccattg cttttcatga aatcgattat ctggtgacaa
    10801 gtaaagaact gacaaaagaa cagtatgcga aagtaaagga acaccatatc gaatgtctgt
    10861 acggataaat tattacgtga atgttctaag tggcttcctc atttttagat aggaccttat
    10921 ctttgctcgc ttctctcagc cgatggtata ataatgaaaa ggttatcttg ttagataatc
    10981 ttactttttt atccaggaga aaggagtgca gaaaatgaaa catgtgaagg cgttggtagt
    11041 gaaggcaatc atgatctggg cgattctgtg ggtcgtgctg acaggtttat acggtgttag
    11101 ctttatggat tcaacgattg ttggggtaat tattgtagtc atgatctatg tgctagggga
    11161 tctgttgatc ttacgtaaag tagggaacat cgcagcgacg atcgcagatg ctggttcagc
    11221 tgcagttatc ttgtggctct atctatattt tatgaatgat acagtcgata tttggatgaa
    11281 agtattgatt cctgctctat tgatcggtgt ggcagaatgg ttcttccaca agtggttgct
    11341 aagtcaaggc atcgtaccag atgaacggaa aatggaataa atcaacagtt aaactattct
    11401 tatcgataaa tgaaagcttt aaaaataaat agatgtttaa ttaagaataa tcataaacta
    11461 tttttactca aaaaaccgct cagattattt tcagatctga acggtttttt ttatctattc
    11521 atcaagatca aatttcattt ttcaatgatt caatcaattc gccctctgct tcatgactat
    11581 gttttcggcc attaacaaga tagagtgctg tacatatcaa tggaatgact ccggcaatca
    11641 catacaatcc tctaaatgaa gtaagagatt ttagcatccc catcaaataa ggaccaacgc
    11701 ctaagcctag gtctaaacca ataaagtagg tagataaagc aatactgatt cgatgttcgc
    11761 ggacgatttt taaacaaaca gcctgtccat ttgacataaa cgtaccataa cctaggccga
    11821 ctaatgctcc agagataagc aatgtcacac tattcgtcgt tacactcagt aaaaataatc
    11881 ctgctgttaa gaataaatag ctaggataca tcacgtattg ttctcctttg acatcgaaaa
    11941 tccttccagt caatggacgt gtaaatgtga tgaccaaagc atatacgacg aagaaaaatg
    12001 acccagcagc aactaagtgg attacttttg tataagaaga taaaaatgat aaaacacttg
    12061 aataagaaag acccatcaaa aaggcaattc ctgtaatgaa aagtgctttc ttttcaataa
    12121 aactattgat attccatgag cgaagagata ttttttgttc ttcagataat ggaatatttt
    12181 ttacaggaaa gacaaggcag gcaatcgtaa caaagaagac cagtacaatt gaaaaccaga
    12241 taatgaaacg aaaatcagtc atattcagca aaatcattcc taaaaatggc ccaacagcgg
    12301 cagctaaact agtacttaat ccataataat tgatgccttc ccctttttta gatgcaggga
    12361 tatatgcagt aacgatagca tttgtagctg tagaaactgt accgtagccg aatccattca
    12421 agaatcgaac taaatacaat gccccgatag ttggcgtata aagatatgct aatgttgaaa
    12481 ggagatagaa gatcgcgccg cctcttaagg taaacttccg accaaatagt tctaactgtt
    12541 tccccatgaa taagcgtgcc agcaatgttc cgatgatata gatcccagaa gcaaaacctg
    12601 cttggctcaa gctggcgtgt aaggtgtctt gcgcaatgac tgccatgatg accatcaaca
    12661 gataataaat caggtagaca ataaaattga tcaatgtgat tccaataaaa tatttattaa
    12721 ataatttctt ttccatgtat gttatacatt ccccttaagc tgaatatgct tttaattttt
    12781 gtatttgaac ttctacatcg tgataattgg atggatagcg atcaacaagt tggcgaataa
    12841 ataaatctct tgtagacata aacagattta aaaattcttc gcattcgttt gttggattct
    12901 catccactgc tttgatcaat tcgatcgagt tttgatacgc atcatttaaa aagactaaag
    12961 aagctttcat atcaaatctt gtttgatatg ctgtttctaa tcgattaatg attgtgttca
    13021 tccagtaatt catgtttttt ccctctattc ttaaaaaatt ttacttctgc agtataacga
    13081 ataaaaaaaa ggaaaaaaat cgcataagtg atggaaaaag gtacttaaca aatgttaata
    13141 tcattagtaa aatacctttg gactttaaaa agttttaaag tactgtatga tctttttaga
    13201 tgaaagtttg gatgggggag catcattgga tagtagagtc ttaaaggaat atttggataa
    13261 aaatgatttt ccgattatac gcaaaggata tcataaatat ttgacattcg aagggatgga
    13321 agattcccat acgtatatct tgaaagaggg aacggtaaaa gcaagtgtta tttcaaatga
    13381 tgggagagaa tttaaccttc gctatatcaa agatcttgaa atcgtctctc ttttaaaaga
    13441 cgaatattca caattcatcg attcgccgta taatattcga gttgaatcag aatatgcaga
    13501 gttttatcga atcaatcgtg tgcgcttttg gcaaaatatc aaccaagacc caaagctaca
    13561 acactatgta aaagagtatt accgctatcg tctgctttat tcgatgaaaa aaatgcagca
    13621 aatgctatta aacggaaagc tgggagctgt ttgtacgcaa ctttacgaat tatatgattt
    13681 attcggtgtg cggacagaag aagggatgct gatcgatttt tcgatcacga atgaagaaat
    13741 cgccaaattt tgtggcatct caactgctag cagtgtaaac agaattttaa aacaactgaa
    13801 aaaagatggt attatcaccg ttaaagacca tcatatcgtt attttgaata tggacttgtt
    13861 agaagacaat attatctttt gatgaacaaa gaggttgtga aatcagcgtt ttgacctgag
    13921 tattagagaa acaaacggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
    13981 gagggtcagc tgattgaaca ccgtttacca agaggttgtg agcctgccgt ttaactctga
    14041 gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga
    14101 ccgaggagtt ggtaggagaa caccgtttac caagaggttg tgaaaaagct gttctgcatc
    14161 aagtaataag aagagccaag taaaaacagt ctcttatgtt tttaacttgg ctggacttat
    14221 caccgtagat gtaagctttt gaacgccgtt tatttgcaac aaaaggggct gtgacacgat
    14281 ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc
    14341 caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag
    14401 gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt
    14461 tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca
    14521 attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa
    14581 cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt
    14641 cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca
    14701 ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta
    14761 agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt
    14821 agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg
    14881 cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta
    14941 cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt
    15001 ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag
    15061 tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaac tttttcatca
    15121 tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt
    15181 cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt
    15241 cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac
    15301 cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt
    15361 caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc
    15421 agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc
    15481 ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc
    15541 gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag
    15601 cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg
    15661 ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc
    15721 ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt
    15781 gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc
    15841 gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac
    15901 gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc
    15961 tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc
    16021 cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc
    16081 ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct
    16141 ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc
    16201 tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa
    16261 cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta
    16321 aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg
    16381 gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg
    16441 ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc
    16501 tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg
    16561 tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga
    16621 accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt
    16681 ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct
    16741 aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt
    16801 gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga
    16861 atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga
    16921 aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc
    16981 agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc
    17041 gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa
    17101 gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt
    17161 gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc
    17221 gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat
    17281 ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat
    17341 ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc
    17401 tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt
    17461 gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca
    17521 ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa
    17581 ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata ccataggaag
    17641 atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata
    17701 cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata
    17761 gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg
    17821 ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc
    17881 tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa
    17941 gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga
    18001 gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat
    18061 cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc
    18121 aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct
    18181 ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat
    18241 attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca
    18301 gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt
    18361 gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga
    18421 aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac
    18481 aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag
    18541 ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat
    18601 tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga
    18661 cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag
    18721 tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat
    18781 tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt
    18841 cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt
    18901 tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat
    18961 caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac
    19021 aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct
    19081 gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat
    19141 gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc
    19201 tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga
    19261 agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga
    19321 tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc
    19381 acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt
    19441 gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt
    19501 tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc
    19561 gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg
    19621 cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg
    19681 tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg
    19741 ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga
    19801 tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag
    19861 gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac
    19921 tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct
    19981 tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg
    20041 taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg
    20101 aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag
    20161 atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg
    20221 tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc
    20281 ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag
    20341 aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc
    20401 tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga
    20461 aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat
    20521 ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg
    20581 ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag
    20641 tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga
    20701 tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc
    20761 tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag
    20821 ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc
    20881 gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc
    20941 cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa
    21001 acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac
    21061 gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa
    21121 gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc
    21181 aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga
    21241 caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact
    21301 atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga
    21361 tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt
    21421 tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga
    21481 agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta
    21541 ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc
    21601 agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg
    21661 agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc
    21721 aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag
    21781 aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca
    21841 gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga
    21901 tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag
    21961 agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta
    22021 ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc
    22081 atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa
    22141 acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact
    22201 tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc
    22261 aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct
    22321 tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag
    22381 tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca
    22441 atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg
    22501 caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga
    22561 tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt
    22621 attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat
    22681 tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga
    22741 attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga
    22801 aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg
    22861 aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa
    22921 agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga
    22981 agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt
    23041 aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc
    23101 aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga
    23161 atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg
    23221 taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg
    23281 acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga
    23341 gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa
    23401 atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata
    23461 caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg
    23521 cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa
    23581 aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg
    23641 acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa
    23701 caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca
    23761 gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc
    23821 gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc
    23881 cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat
    23941 ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat
    24001 tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat
    24061 cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc
    24121 tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc
    24181 atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa
    24241 gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac
    24301 attgacgaat tttttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac
    24361 aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga
    24421 acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt
    24481 gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc
    24541 aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca
    24601 agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa
    24661 gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt
    24721 gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc
    24781 aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca
    24841 tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg
    24901 tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag
    24961 tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa
    25021 atacagcgat atttccaaac aagtacggga agtattctat cgttatacag atattgtgga
    25081 accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc
    25141 agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg
    25201 ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc
    25261 tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat
    25321 tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta
    25381 tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat
    25441 gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag
    25501 agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc
    25561 agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca
    25621 aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa
    25681 aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct
    25741 tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac
    25801 gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat
    25861 ataaaaacag ctttgagagt tgaaaccgga aatccgtttt ctctcccaaa gctgttttta
    25921 tatcaagtct tgctttttga tttgttcata ggtttctgtt gcatccgtac atttttccca
    25981 aatcacaact tgattggatt ctaaagattt ttggacgtca atgatccgct ggttgctact
    26041 tccgcgaaat tgtagtttta ggtcacgttt gcttaattca aagcgtccgt caacaaggat
    26101 atcgatttgc gaaaggagtt ctaatttatc ttctgaatct tgtagcaatt catcaaaggc
    26161 atagcctgtc catgaccaga tatcttttgt cgtaccgaac tcttttcgga tacgtcgtac
    26221 gacttgcaga caaacatctg tattcaaaaa aggttcgcca ccaagtaaag ttaatccttg
    26281 cacgtaatcg ttgcgcaagt cagcgatgat ttgttcttct aattcaggtg tgaatggttt
    26341 gccatatcgg aaattttgtg cagctttgtt gaagcagcct tcgcaggcaa ataagcagcc
    26401 actaacgtaa agactgttgc ggacaccttc accatcgaca aaattaaacg gtttgtagtc
    26461 agcaatatag ttttggctgt agtcttttgc taaccattct tttggttttg gatttctcat
    26521 aaaaaaactt ctttcattta aggttcaaag atatagctat tgaagataaa atctactcga
    26581 ccacataaat aagaacaaac tgacctaaat ccattgttca agaaagcttg ttcttatttt
    26641 gatagagggc gttcaaagat acaaagccat cgaaagaggg tggtgaaaaa agacttgtca
    26701 tagcctctta ttctgaataa acggtgttca aaagcttgca tctgcagtaa taagcccaac
    26761 taaacgaaaa atatgaggaa ctatttttgt ttagctattc ttattacttg atgcaggaca
    26821 gctttttcac aacctcattt catgtgtttc acacgtgagg aaatttcttt atggcggcca
    26881 tgtaccatcg gacgagcttg cggatttcct aaatagccac aagtacgttt gactacatca
    26941 catgtttttg gatcatgatt tccacattct ggacattcga accctcgttc tgtcggtttg
    27001 aaatcacctt cgaaaccaca ttgatagcaa tgatcgattg gcgtattcgt accaagatac
    27061 ccgattttgt catatgcaaa atcccataca gcttctaaag ctttcggatt ttgttggagc
    27121 atagggtatt cacagtagtg gatgaatcca cctgaacaat attttggata atccttctca
    27181 aattccaatt tttcaaatgg cgttgggttt ttacgcacat catagtggaa actgtttgta
    27241 taatattctt tatctgtgat attttcgatg atgccgaatt tttctgtatc caaacggcag
    27301 aaacggtcag ttagactttc acttggagtt gaatagacac tgaaatgata accatattca
    27361 tccccccatg catctgcatt agcttttaat tctttgacga tatcaagtgt gaaagctttt
    27421 gcttctgggt ttgtttccca ttcgccaccg aagaaagctg aagcaacttc atacaatcca
    27481 atgtagccta acgatacagt agctcgacgg tttttgaata attcattgac agaatcggta
    27541 cgtgaaagac gtttcccgaa agaaccatac atatataaaa ttggcgcatt tgctggaatg
    27601 gcttctttac aacgttctac acgatagaga agcgcatctt tcatgatttc taagcggtca
    27661 gacagtaact gccagaattt attctgatct ccttttgatt cgatcgcgat tcttggcaaa
    27721 ttcaatgtaa caacacctaa attcattcgt ccgacattta cttcttgacc attttcgtct
    27781 ttccaccctt gcaagaatga acgacagccc ataggtactt tgaaacttcc agttaattca
    27841 acgattttat cgtaattcag aatatcagga tacattcttt tcgttgcaca ttccaatgct
    27901 agttgtttga tgtcgtaatt aggatcttca gggtttaagt tcacaccttt tttcaaagtg
    27961 aagatcagtt tagggaagat agctgtccgc ttttcactcc ctaatccatt gattcgaatc
    28021 tgtaaaattg ctttttgaat ttctctttca aaccagtttt ctcctagtcc aaatcctaat
    28081 gaagtgaaag gagtttgtcc atttgaagta aataaggtat tgatttcata ttccaaactt
    28141 tgcatagcat cgaagatatc ttttttggtt tttgcttttg catattcttt ttggcgttct
    28201 ggcgaatcga tccattcttc cgcgtctttt agatgttttt tatagttcaa ttctgcaaaa
    28261 ggagcaagca attcatctgt tcggtcggca gaacagccac catattgact tgacgctaca
    28321 ttggcaataa tctgtgagat ttgtgctgtg gcggtctgga tagattttgg cgattcgact
    28381 tcggcattgc cgattttgaa cccattgttc agcatgcctt tgaaatcaat caagcagcaa
    28441 ttcgtcatcg gtgtataagg atgataatcc aaatcatggt aatggatatc gcctttttga
    28501 tgcgcatttg ctatatgtgg tggcaacatt ttcagtccga ttgattttcc aacgatcccg
    28561 gctgtcagat ctcgctgtgt attgaaaaca ttactgtctt tatttgcgtt ttcgttcacg
    28621 acagtttggt ccttgttgat tagcttttca atcgaaaagt tgatatctgt tgctttactg
    28681 cgagcaaaat ctcgtttggt acgataattg atgtactctt ctgctaattc atattcgttt
    28741 ttttctagta atatatgttc cacgatgttt tgaatctcat agattttgac attttcagca
    28801 aaccggcttt tgatttcttg gacaaccaag tctgcaatca tttgaatttt ttcataagta
    28861 agtggtgata atgagccgtg aattttcttt tctgctttga tcagtgcttc attgatcttt
    28921 tgttcatcga aatcaacatg gcggccatcg cgtttgatga ctttcatctg ggatgtctct
    28981 ggctgctgag taacgacagt tttttcttca taatttactt tcattatctt caactcctgc
    29041 taaattgttc ttgtctatca taaaacaaaa tcggggaagg agcaactaaa tatcggctat
    29101 atatagtatt caatgtgctg tataattgga tttctatact atatgtagtg tatattttat
    29161 tgtgaaaaaa ggtgaaaact tgtagctggt ggatttgttt tttgcatatt taattttgct
    29221 gaataaaata attgaataaa attttttatt cagtccaagt aaaagtgaca aaaacataag
    29281 agcaaattat tcgtagatat gctactatgc aaataaatag aaatgaaaaa tgaaagagga
    29341 ggagaagaat tggaaaagat gatcgaagtg aaacatctaa gtaaaagcta cggcagcaaa
    29401 ctttctcccg tcaaagcctt agataatatc tcattcacag tagaagaagg cgagtttgtt
    29461 gggattatgg gcccaagtgg tgcaggaaag acaacgctga tgaatatctt gtccacgatc
    29521 aatttgccta cgatgggcag tgtctcgatc caaggaaaag aaatcacaaa aatgaaaaac
    29581 agtgagctca gtgactttcg tcgaaaaaaa ctaggattca tttttcaaga gttcaatttg
    29641 atcgataccc taaatgcaaa agacaatatc ctattgcccc tagcagttga acggatgact
    29701 aaagaggaaa tggaaaagag agtaagacat gtagctcaat tgttgaatat agaagaactg
    29761 ctcaaacggt atcccgatga actttctgtc ggacagagac aacgaattgc agctgctaga
    29821 gcacttgttg ttcagccaca agtgattttt gcagatgaac caactggctc tttggactcg
    29881 aagtcggcta cagaattgtt gaactacttg aagaccatga atcaaaaaga aaaagcaacg
    29941 atactgttag tcacccatga tccatataca gcaagctact gcgatagaat tttatttatt
    30001 aaagatggtg tgattttttc tgaagttgtc agaagaggga caagaagaga gttctttgag
    30061 aaagtaatag acatgcaggc gacgattggc ggaggaggaa aaatgaatgc tgtataaact
    30121 agccgttaaa agtttttttc ggcagtcacg aggctatctt gtttattttt tcagtttgac
    30181 tttatccgta atgatctatt attccttcag cgcaatgacc tacgatcaat ctttagtcag
    30241 aagagcaagt caagatactt cgatagatgg tggattgagt tttggtggat ttatcattac
    30301 tgttgttttg ctttttttcg tattcagtgc caaccgattt ttcttgaata gaaggcaaaa
    30361 ggaaataggc atctatcagc tttttggtat gaataaactg cagatatcag gtatatatgt
    30421 gttagaaatc atgataattg gattgttcgc ctgtatttct gggatattac tcggtattat
    30481 tttttccaaa ctcttttcaa tgattttggt tcgaatgatg gatatggatc ttactagtcc
    30541 attttttatc tccattccat cagtagtaga tactttattt gcttttttca tcatcctttt
    30601 agcagtatct gtatattcaa tatggaaaat atggcgctat ccaatgattc gaagttttgg
    30661 agaaaaagaa caagcagata gttcaacaat gcgcttcagg acaaggcacc gattactcgc
    30721 agtcatcggg ttactacttt taacgagcgg atattttgga gctagtcatt ttagggaaat
    30781 cacttcatgg atgatcagtt atcattatct tgatacatta gttttctttt ttcctttttt
    30841 aatttttttc gtttgtgtgg tcggtactta tttgtttttc tgttatacat ttcggttatt
    30901 tttgatcata ttgagtaaat ggaagattta ttactatggt atcaattttt tgataatggg
    30961 taacacgcaa gttcatttgc tcaaaagttg gcgtacaaat tctctgatta cagtcattct
    31021 cggaatttct ttaatgacaa ttggcggcac ggctagtatc tctactattt tatcctacca
    31081 ggagaaagtt tcctccccga tggactatca gtttgatgtt gagacagaga aaaaaatcaa
    31141 gccgattcta gcagaggaag ggcagaagat aactaaagaa atcaccttgc attataaagt
    31201 cattgggagt atctatagtc aaaatctcga agaacctatg tttcaaactg ctaatctgat
    31261 cacggaaaca caatatcaag ccttcagaaa agttaatcca gatttgccga acatttcttt
    31321 gaaatcacct ggtcatactg ttttgcttga tcaaatgcaa actctttttc ggaatatttc
    31381 attatatgga tctagtattg gactgccaga taatcaagaa ttgaagattc aacaaatgct
    31441 tccaagtgta ttaggtgacg aaacaatggt atatctaagt cctgtactga ttgtagatga
    31501 taccgttttt gaaaaagcaa aaggtgtgga ttatcaagta gtcaatgtag atgtatcagg
    31561 tggaaataat gaaaaaatag atgaacgact gagcaatgac ctgtcgataa agtggctcaa
    31621 tgctgtttat tattcttatg atatcgtaaa tggaagtcta caaggagaaa ttagcacaaa
    31681 gaaacttccg gatgaccaaa tgaaggaagc acaatttagc caagaaagct ctcgtttgaa
    31741 tttctcttcc agctattcga aattgcgtgc agttaatcgg cgaatcggta tcaatatttt
    31801 tgtcacacta tttgtcggga tgatcgtgat tattgctact ggtagtattt tgacagtccg
    31861 gcaattagca gaagcagagg aagaaaaaga aaattatcac ctattgacta agctggggat
    31921 tccacaaaga aggatcagac ggatgatctt cgagcaaaac gcacttacct tctttccacc
    31981 catgatatta gggatcactc acgcagtatt tgcaatcaat gtcttcactc agtatataaa
    32041 aactgccgat tactggttgg cttatcttgt gagtggctta ttgatcctta tctatctttt
    32101 gctttatgtc attacgagtc agttatattg tcggattatc gaggaatgat atacggaata
    32161 agacaagata ataaatagaa ctaaattttg aataccattt atttggaata agaggttgtg
    32221 acacgatttt tgtcacaacc tctttatttt ttatattttt taaaagtacg agaaattata
    32281 aagaaaaaag cagtgatttg atgtgctatt tttataaaag ctatgtgaca aaactgttag
    32341 taaaaaaacg gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt agttacactt
    32401 tgtgtgaggt tatatgagga gggaatccaa tgaacaaatg gcgttctaat ccaaaattga
    32461 attattgggg aaaattcatc attcgaacaa ttttttatag tgcagtactg ctgttcctga
    32521 tttatctgta tcactataaa aatattcaag gaggaacttt tatctataac gaattttagg
    32581 aggaagagta tggaaatcaa aacgattatt gaagcaatcg atgattgggg aataaatgaa
    32641 cctgatcgag ttgcttatca ggcggatgag acgcatactt ttggtgaatt gaaacaagca
    32701 tcagacgcac ttgcttatta tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt
    32761 tttggcaatt tggagtttga aatgattgtc tcatttctag gggttgtcaa atctggccat
    32821 gcctatttgc cgattgaaga gcatacaccc aaggagcgta tcctatcgat ctttcgggtg
    32881 gctaaaccat ctatggtcat cagtatcggg gactggatag aagatattcc ttcggttcct
    32941 gtcataacaa aaaaagaatt tcaagaaatc aagaagatac cagtaggatt ttatcccaga
    33001 aatagcgtaa aaggtacaga aaatttttat attattttta cttctggtac tactggagaa
    33061 ccaaaaggcg tacaaatcag tcatgataat ctagtcagct ttgttcaatg gacgatggaa
    33121 gactttggga tcaagcctgg tatgcatttc ttagcccaag cgccattttc ttttgattta
    33181 tcggtatttt ctatttatcc tgcattagtt tctggcggaa tgctgaagcc gttaaataaa
    33241 gcagttgttc aagactttcg tcaattattc gcgacattgc cagccttgaa gttgaatgtg
    33301 tgggtttcta ctccatcatt tatggatatt tgcttgatgg aacctacatt caatggagaa
    33361 aatgtgccgg aattggacat gtttttattt tgcggagaag aattgacgaa aaaaactgca
    33421 gaaagtctac ttgatcgatt cccaaatgca cggatatata atacttacgg tccaacagaa
    33481 gcaactgtag ctatttcaag tattcagatt gaccagcatg tactagatag ttatgaccga
    33541 ctaccgattg gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga aggtaaacct
    33601 gtttcaaaag gagaaacggg tgaaatcatt attgcaggac caagcgtatc aaaaggttac
    33661 ctgaacaatc ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt agcaagttac
    33721 catacagggg atgcaggtag actagatgaa tatgggcttt tattttatga aggacgtatg
    33781 gatcagcagg tcaaattaca tggataccgg attgaactcg gggatatcga acattatctg
    33841 ctccaagaca accgaatcaa acaagctgtc gtcgtaccaa aataccaagg aacaaaagta
    33901 cagcagctgg ttgcttttgt cgtattagaa aacaaaacga aacagccgga ttttcagttg
    33961 actaaatcta tcaaagaaca attactagaa gttgtaatgg attatatgat tcctcagaag
    34021 ttccattatg tcgatctgct gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta
    34081 acagctgagg tgaatcctga atgatgttac ctcatatgat cccttatgca gagccgtttt
    34141 atttttttct tttagccatt gcgcttgtac cgatcgttct atctttattg atatgggaaa
    34201 aaagattacc agtttatcaa tcagcagtga cacttttctt tttatttgtc agctttggag
    34261 gtaattattg gcaacaggga gcggcgctga ttgggtatgt tttgtggcag acgatacttg
    34321 tatggggcta ttttgtttat cggcaaagga aaaatcagac aggatggttt tttgccgccg
    34381 ttattttagc gataattcca ctgtttttaa cgaaagtgac tccttttttt gaacatggaa
    34441 aagcatcgtt acttggtttt ttaggaatct cttatttaac ctttaaatct gtacaaatga
    34501 tcatggaaat aagagatggc atgatcaaaa catataagcc atttcgttat atccagtttc
    34561 tgctgttctt tccaacaatc tcttctggac caatcgatcg ctataggaga tttgaaaaag
    34621 atcagctcca tccgcctgtt cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa
    34681 atatttttgt cggtttttta tataaattca ttatcggtta ttattttggt caagtactgc
    34741 ttcctgtagt gggacgcatt gccttacagc atggtggtat ttcatgggca ttagtaggtt
    34801 atatgtacgt gtacagtatg tatttgtttt ttgattttgc tggctatagc ttatttgcag
    34861 tcggtacaag ctacatgatg ggatatcaga caccaatcaa tttcaataaa ccttttttat
    34921 catggaatat caaagaattc tggaatcgtt ggcatatgac gctgtccttt tggttccgtg
    34981 attatgtata tatgcgattg atgttcttca tgatgaagaa aaaatttttt aaaagcaaga
    35041 tcgtaatgtc aaatatcggt tattttgctt tgtttcttct aatgggtata tggcatggat
    35101 tgacgtggta ctacatcgta tatggactgt atcatgcttg tctgatctgt ctgacagatg
    35161 catggatccg atttaagaag aagcataaag agcagttgcc ttctaacaaa tttacgcatg
    35221 gcttagccgt atttttgaca tttcaagcag tatgttttag cttcctgatt ttttcaggat
    35281 ttttagataa actttttttt agctaacgaa tagtgacata ttaatttata taacaaagga
    35341 gaaaaggaaa atggaagaac aaattttatc aatcttagaa gaaataacag gaacagatga
    35401 agtaagaaaa aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc
    35461 tgttcaatta ttagtcgagc tagacggaga gctggatatc caagtccctg tatctgaatt
    35521 cgagcgagag gattggagca caccgaataa aatcattcaa caagcaactg cattgaaggg
    35581 ttgagtatga aaaagaaact ttttgctatt ttcggcccgg ttgttttagc tgttggctta
    35641 ctgtttgtgt tttttacctc ctcctatcaa gtagatatct ctaatccaga aacgattaga
    35701 cgtgcctctt cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa aaattatgca
    35761 ttatctgaaa aacaatatat tccttttttt ggctcaagtg aattaagcag aatcagtcca
    35821 tttcatccgt ctgttctggc agaaaaatac cagcgaaact atcgtccatt tttgctggga
    35881 gcacccggga cacaatcgtt aagccaatat atgatgatgc gttctgcagg agatgctatg
    35941 aaaaacaaga aagtcgtctt tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa
    36001 actgattact ttaatacgta ttattctgaa cttcaaacat acgactggtt gttttcaatg
    36061 aagaaagtaa cgcctgctga tcgctattta gctagaagat tattaacttt ctcaaaagta
    36121 aaagagaatg atacgttgac ggcgattctg caaacgatca aaaaagggaa gctaccttta
    36181 cctgaatctt tgaatcagct gcgcagccaa tggaacatgc tgaagagaga ggatgaagta
    36241 ttcagcaata ttgggttgaa agatcgacag caaaaaatcg atcatgagtc aaaacgatta
    36301 ccaaaacaat accaagaaac agaattatcc attttagcaa atcaaattgg agaacgagaa
    36361 acgacgaata atccattcgg gttgaaaaac gatttttata cgcatcgtat ccgagcccac
    36421 gagccagagc tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc agaattttct
    36481 gattttcagc tggtcttgga tcaattagca aaaaaccata acgaagtatt gttcatcatc
    36541 ccaccggtta acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga
    36601 tttgccaaaa agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt
    36661 gtcaatcaag cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc
    36721 tggttggctg cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt
    36781 aagtatcatc ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa
    36841 ctacaattaa aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat
    36901 attctgaata aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat
    36961 aatcacaata tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac
    37021 ttattgattt aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat
    37081 tcagagaata tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga
    37141 ttgccaaaca tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag
    37201 ttggttttat ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag
    37261 gttggtgggg gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa
    37321 tcgtccacgg tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata
    37381 aaccttatga aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca
    37441 aagagtgggg catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga
    37501 atggcagtaa atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc
    37561 tgcgagtatt agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg
    37621 atgtgcaaga agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg
    37681 atattgcttt tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag
    37741 aagcagattc gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc
    37801 cgcataaaaa attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc
    37861 aatatacagt gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg
    37921 gtgttccgtc tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga
    37981 ttcatgaaga tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg
    38041 atagtgaaac agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc
    38101 tgaatatcgg aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct
    38161 tgttttctta tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc
    38221 ttttccggaa gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt
    38281 attcgcattc aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc
    38341 aaaaagtaac cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat
    38401 caatttgatc aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt
    38461 tttaatcagc ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac
    38521 gggtgtcatg ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat
    38581 ttttgtttca gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac
    38641 aaagttcgac tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt
    38701 gaaagataga attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct
    38761 aggagcaatc gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt
    38821 tgcagaattt tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta
    38881 gcatgtaagc agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg
    38941 ggaatgtacg atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca
    39001 agggacaaca ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag
    39061 tgctacgctg atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct
    39121 tagccattat catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact
    39181 tcatcctaag agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat
    39241 tcactttgaa gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga
    39301 agaattaaaa attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg
    39361 ttacgccatg cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc
    39421 aggttatttg gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac
    39481 ctatcttttt gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga
    39541 acttgcgaaa tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga
    39601 tttagaacaa ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact
    39661 tgctgcagtt gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg
    39721 aaaaccacga cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt
    39781 tagatgaacc tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc
    39841 aaaatacgct tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc
    39901 gtcgcttaag tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta
    39961 tcatgcctga tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc
    40021 aagcacttca tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca
    40081 ttgatggcat gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc
    40141 atggaacaat tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa
    40201 aagacaaaat cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac
    40261 catttcttcc agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt
    40321 tgaaaatttt cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag
    40381 attggaaaaa aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg
    40441 ggaagtctcc agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag
    40501 tccatttgaa tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg
    40561 gactaggaga tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat
    40621 ctctatcaaa aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag
    40681 aagaattgct taccttactt tattgaaaat aaaaaatagc ggaatccaca tatatggatt
    40741 ccgctatttt ttatacagga ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa
    40801 tgaaagtatc tttttcctgc gttagtgttc ctgtgtaacg atccgctacg tctttcatca
    40861 tcattaagtc aaaacgttcc tgatcatcta attcttctgt gatgcctcga ctaaataggt
    40921 ttgtatcgta atgtgcacta tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta
    40981 tcgacaaata aatttttcga tacagtggat tttcgatggc atgagtttct ctaaaggaat
    41041 gctctaaagc gtggatcaaa agttgagcca gatcgtcatc gttgatagga agttcttcgg
    41101 gaagatctgc ttggaaggtt atctgaatat tccgtttggc agctaacttc tggtaacgcg
    41161 ctaagataag atttgtcacc tggtggttgc aaaaagaaca cagcatttcg aaattttttg
    41221 cttcttctaa tatcttttcc acatagtttt tagcttttgt ttggtccgta tgcattaaat
    41281 cttgcagatt ctccatctct tggacaaatt ccaggcgaag tgtatttact tcttggatat
    41341 gctgtcgaag ctgctcataa tgttgctcca acaaatcatt tttgattttt aagaaatcta
    41401 cttgtctccg atctttgtcg gtatttaaga tgtgatcgat tagattgtag ctgataataa
    41461 cgattagaat gataatatat aaaatcgtag accaattgat cgttgtttga tagataccta
    41521 atgccgtttg gatgtaaatc aagcaatgga taatggctac caagacgatc caaggtgtac
    41581 aagctacata aaaacggtta ttcttatatg cttcgccaat gcatgcaagt gaagtgacta
    41641 aagtgcttat aacactagca atattagcaa tcggcattaa ttcaggaagt tctgcaggag
    41701 taaaagttgc ataaagcaga acgccgataa aaatcaaaag ttggaacgta caaaaaattc
    41761 cgtaccattt tttatagtaa aacattcggc tccaatagta gcagatgatg aaagctgacg
    41821 ttaagataat aaataaatga gataaaaaag aatgaacgaa tggttcaaaa agaataccac
    41881 ttaaaatatc ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa
    41941 taagtgagta ttcaagacgt ttttgtttat acatcaagta gatcgtatag aaaaaggtcc
    42001 ctatagcgat aaaaacagcg attatcatcg tcaatacttg cggcactgat tgtgaataaa
    42061 taaaactaat caatggtcca gttgttccta gataaacttt cggtggcagt ccagcatagt
    42121 tctgataagg agatgagact gtaagggtca atttttttcc gatataattt gctggtaaat
    42181 ctatcacagc caatgagagt ccgggattgc tttcttcctg ataatctttc cgttggtata
    42241 aaatcttttc gcctagatat acagtaatcc attgatggtt tcctttgatt agtaaatttg
    42301 cttcgtccaa cggtttttca agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg
    42361 taggaatcgt acgagtgtac ttgctgtcaa acttaaaatc aggatcatct tttgtataga
    42421 aaacccacga atcttttatt tctttcattt gattgctgtc cgcaggagaa aaaataaaat
    42481 aaaagaaata agcaattcct gttaaaaata acaatgataa tataatgatt atgctccact
    42541 ttatacgtaa a
//
LOCUS       DARDHU010000012        40414 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000012 DARDHU010000000
VERSION     DARDHU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.012"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SDD03_000723"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SDD03_000723"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG4303194.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SDD03_000724"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SDD03_000724"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG4303195.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SDD03_000725"
     CDS             complement(2303..2965)
                     /locus_tag="SDD03_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4303196.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SDD03_000726"
     CDS             3149..4795
                     /locus_tag="SDD03_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG4303197.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SDD03_000727"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SDD03_000727"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4303198.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="SDD03_000728"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="SDD03_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="SDD03_000729"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="SDD03_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="SDD03_000730"
     CDS             complement(8944..10023)
                     /locus_tag="SDD03_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4303199.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="SDD03_000731"
     CDS             complement(10126..10566)
                     /locus_tag="SDD03_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4303200.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="SDD03_000732"
     CDS             complement(10623..11033)
                     /locus_tag="SDD03_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4303201.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="SDD03_000733"
     CDS             complement(11213..11647)
                     /locus_tag="SDD03_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4303202.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="SDD03_000734"
     CDS             complement(11747..12595)
                     /locus_tag="SDD03_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4303203.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="SDD03_000735"
     CDS             12984..14750
                     /locus_tag="SDD03_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG4303204.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="SDD03_000736"
     CDS             14852..16111
                     /locus_tag="SDD03_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4303205.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="SDD03_000737"
     CDS             16258..17559
                     /locus_tag="SDD03_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4303206.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="SDD03_000738"
     CDS             17562..18413
                     /locus_tag="SDD03_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4303207.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="SDD03_000739"
     CDS             18426..19241
                     /locus_tag="SDD03_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG4303208.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="SDD03_000740"
     CDS             19392..20558
                     /locus_tag="SDD03_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4303209.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="SDD03_000741"
     CDS             20788..21435
                     /locus_tag="SDD03_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG4303210.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="SDD03_000742"
     CDS             21519..22463
                     /locus_tag="SDD03_000742"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG4303211.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="SDD03_000743"
     CDS             22598..24049
                     /locus_tag="SDD03_000743"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG4303212.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="SDD03_000744"
     CDS             24070..24906
                     /locus_tag="SDD03_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG4303213.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="SDD03_000745"
     CDS             24906..25565
                     /locus_tag="SDD03_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4303214.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="SDD03_000746"
     CDS             25691..26380
                     /locus_tag="SDD03_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303215.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="SDD03_000747"
     CDS             26386..27342
                     /locus_tag="SDD03_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG4303216.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="SDD03_000748"
     CDS             27386..27898
                     /locus_tag="SDD03_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4303217.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="SDD03_000749"
     CDS             complement(28154..28702)
                     /locus_tag="SDD03_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4303218.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="SDD03_000750"
     CDS             complement(28791..29972)
                     /locus_tag="SDD03_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEG4303219.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="SDD03_000751"
     CDS             complement(30208..31716)
                     /locus_tag="SDD03_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG4303220.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="SDD03_000752"
     CDS             31875..32066
                     /locus_tag="SDD03_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303221.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="SDD03_000753"
     CDS             32269..33186
                     /locus_tag="SDD03_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG4303222.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="SDD03_000754"
     CDS             33179..34252
                     /locus_tag="SDD03_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303223.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="SDD03_000755"
     CDS             34400..34918
                     /locus_tag="SDD03_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303224.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="SDD03_000756"
     CDS             34899..35291
                     /locus_tag="SDD03_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG4303225.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="SDD03_000757"
     CDS             35367..36302
                     /locus_tag="SDD03_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG4303226.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="SDD03_000758"
     CDS             36435..37214
                     /locus_tag="SDD03_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG4303227.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="SDD03_000759"
     CDS             37207..37524
                     /locus_tag="SDD03_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG4303228.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="SDD03_000760"
     CDS             37768..39378
                     /locus_tag="SDD03_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4303229.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="SDD03_000761"
     CDS             39535..40383
                     /locus_tag="SDD03_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4303230.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DARDHU010000013        39389 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000013 DARDHU010000000
VERSION     DARDHU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.013"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..288
                     /locus_tag="SDD03_000762"
     CDS             4..288
                     /locus_tag="SDD03_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303231.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            448..684
                     /locus_tag="SDD03_000763"
     CDS             448..684
                     /locus_tag="SDD03_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4303232.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            681..1079
                     /locus_tag="SDD03_000764"
     CDS             681..1079
                     /locus_tag="SDD03_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG4303233.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(1746..2690)
                     /locus_tag="SDD03_000765"
     CDS             complement(1746..2690)
                     /locus_tag="SDD03_000765"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG4303234.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(2986..3930)
                     /locus_tag="SDD03_000766"
     CDS             complement(2986..3930)
                     /locus_tag="SDD03_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303235.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            4150..5364
                     /locus_tag="SDD03_000767"
     CDS             4150..5364
                     /locus_tag="SDD03_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4303236.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            5386..7401
                     /locus_tag="SDD03_000768"
     CDS             5386..7401
                     /locus_tag="SDD03_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG4303237.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(7484..8857)
                     /locus_tag="SDD03_000769"
     CDS             complement(7484..8857)
                     /locus_tag="SDD03_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG4303238.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(8883..9167)
                     /locus_tag="SDD03_000770"
     CDS             complement(8883..9167)
                     /locus_tag="SDD03_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4303239.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(9169..11184)
                     /locus_tag="SDD03_000771"
     CDS             complement(9169..11184)
                     /locus_tag="SDD03_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4303240.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(11363..11668)
                     /locus_tag="SDD03_000772"
     CDS             complement(11363..11668)
                     /locus_tag="SDD03_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG4303241.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(11757..12788)
                     /locus_tag="SDD03_000773"
     CDS             complement(11757..12788)
                     /locus_tag="SDD03_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4303242.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            12995..13870
                     /locus_tag="SDD03_000774"
     CDS             12995..13870
                     /locus_tag="SDD03_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4303243.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(14022..14864)
                     /locus_tag="SDD03_000775"
     CDS             complement(14022..14864)
                     /locus_tag="SDD03_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4303244.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(14956..16833)
                     /locus_tag="SDD03_000776"
     CDS             complement(14956..16833)
                     /locus_tag="SDD03_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4303245.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(16830..18251)
                     /locus_tag="SDD03_000777"
     CDS             complement(16830..18251)
                     /locus_tag="SDD03_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4303246.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(18254..21643)
                     /locus_tag="SDD03_000778"
     CDS             complement(18254..21643)
                     /locus_tag="SDD03_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG4303247.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            21900..23291
                     /locus_tag="SDD03_000779"
     CDS             21900..23291
                     /locus_tag="SDD03_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303248.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(23364..24011)
                     /locus_tag="SDD03_000780"
     CDS             complement(23364..24011)
                     /locus_tag="SDD03_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG4303249.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(24019..24705)
                     /locus_tag="SDD03_000781"
     CDS             complement(24019..24705)
                     /locus_tag="SDD03_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303250.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(24711..25784)
                     /locus_tag="SDD03_000782"
     CDS             complement(24711..25784)
                     /locus_tag="SDD03_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303251.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            26006..26680
                     /locus_tag="SDD03_000783"
     CDS             26006..26680
                     /locus_tag="SDD03_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4303252.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            26685..28025
                     /locus_tag="SDD03_000784"
     CDS             26685..28025
                     /locus_tag="SDD03_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4303253.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(28174..29160)
                     /locus_tag="SDD03_000785"
     CDS             complement(28174..29160)
                     /locus_tag="SDD03_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG4303254.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            29884..30915
                     /locus_tag="SDD03_000786"
     CDS             29884..30915
                     /locus_tag="SDD03_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4303255.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            31024..32556
                     /locus_tag="SDD03_000787"
     CDS             31024..32556
                     /locus_tag="SDD03_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG4303256.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            32695..33507
                     /locus_tag="SDD03_000788"
     CDS             32695..33507
                     /locus_tag="SDD03_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG4303257.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            33497..33982
                     /locus_tag="SDD03_000789"
     CDS             33497..33982
                     /locus_tag="SDD03_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4303258.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            34005..34550
                     /locus_tag="SDD03_000790"
     CDS             34005..34550
                     /locus_tag="SDD03_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG4303259.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(34623..36266)
                     /locus_tag="SDD03_000791"
     CDS             complement(34623..36266)
                     /locus_tag="SDD03_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG4303260.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(36395..38623)
                     /locus_tag="SDD03_000792"
     CDS             complement(36395..38623)
                     /locus_tag="SDD03_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4303261.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(38656..39135)
                     /locus_tag="SDD03_000793"
     CDS             complement(38656..39135)
                     /locus_tag="SDD03_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303262.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11573 a         7393 c         7156 g        13267 t
ORIGIN      
        1 cttatgggag caatcacgat tgtggctgtg taccaagcat ttctaaagta tcctgattac
       61 gggacgaagt tgttgtcatt gacgcacaat caaacagcat attcaccaac gttgatttta
      121 tcgactttga ctgcaggagc taaaagcgct acttattttt ttcataaaac gacaagcatg
      181 ctaaagcgaa agaaacagcg aaaacaattg ttggtggtgg tagcggctat gatcattatt
      241 tattttctac cagttattac acaagtattt catcaatatt ttggttaaga atacgagagt
      301 ttgtgacaaa agtcatgtca caaacttttt tctttgtcat tttttcgtaa gaaaaagcaa
      361 aataaaagta tatacattga ttaaaagtat atacttggat ataatgtact tactatagaa
      421 tgagaatggt atagagggtg gcgaaatatg aaaaaaatga aaaagtataa aattagaaaa
      481 tctggcaata gcgatgtaac aactatccca ccagaagtaa aagaattcat gggtgtgcag
      541 acaggtgacg caatcagtta tgtttttcaa tctgatggtt ctgtacgaat gatcaaagca
      601 caagaagaac ctgatattga ttcattagtt gattcgatca tgaatcaata tgaagacgcg
      661 ttaaaagatt tggttgattt atgaggtatc tttcgactaa agaaattatc aaaatcaacg
      721 cgaaagttat tttacgtcac tcgccaggcg aaatgatcgg tgtaaaagat gcaaatgcat
      781 tagatatggc cgtcaaacag ccttcacaag cagtattcaa tcaagaactc tatcctgaaa
      841 tctatgaaaa agcggcgatt ttagcaatta atttagctaa aaaacatcct tttcataatg
      901 gcaataaacg aactgctctt gttgccatgt tgttgtttct tcaattgaat aattgtccag
      961 ttgctttttc tcgtcaagaa gctgttgatt ttatcataat gattaccact agttcattgg
     1021 atttcgattc actaaagtcc aagataacca gttatttacg acaaaccgca ataaaatgaa
     1081 cgaattaaaa actttatttc tcaattagat cagcatgtca ttaaggaata tgctgatttt
     1141 tttatgttta atcatacaaa atagtttttt cataaaaata tggaaaatat ggattaattt
     1201 tgaaataatt gatttaaaag gaatacaaaa gagtgaatag taggaaatag tggtttagaa
     1261 ctagtgggct gattttttat atattttagt ttacataatg tacattatac aaagtagatt
     1321 taaaaaatga taaacctctc tcgaacggtt ttttatcaaa aatcgtcatt tttccatcaa
     1381 aatctgctta tgcaaaatta taaaaaaatt ttgcagtttt tcttactttt tttgtcgttt
     1441 aattgaatga taaaaaccag tttctttatt cagttaatca aatatgaacg aattagatat
     1501 gaatcattgt atttgccgtt gcggaaggat tttttaatta accacagata aacattgata
     1561 acgattcgct tattgtaatc aacagattaa atttcatatg ttcatatgta cggatacatt
     1621 ttcggaccga ttaattttaa ccattctgtt tatttgctta cctattagat atcaataaaa
     1681 aactgcttaa cagctatttc agctgttaag cagttttagg atttttagtt catattcatt
     1741 gtttcttata atacagtgtt caatacttct cttaaagcac ttgttgattt gctgaacatt
     1801 gctttttctt cttcgttgat tgataattca acaacttcac gaacaccatt ttcgtcaacg
     1861 attgatggca cacctgtaaa gatatctttt tcgtcgtatt caccagttaa ataagcagat
     1921 acaggcaata cagcttgttc gttattcaag atagctttta cgatacgtgc agtactcata
     1981 ccaataccat agtaagttgc ttttttacga tcaatgatct cataagcagc attacggact
     2041 ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat ggtctttttc tacaatttca
     2101 aacactggtt taccaccgac agttgtatgt gaccatgcag caacttctga atcaccatgt
     2161 tcacctaaga tatagccgtg gacgctgcgt gggtcaactt ttaatttcaa tgcgatttct
     2221 ttacggaaac gagttgtatc caatgttgtt ccagtaccga taacacgtga tgttggcaga
     2281 ccagattcgt tccaagcgat atatgtcaaa atatctactg ggtttgaagc aacaacgata
     2341 ataccgtcga atcctgatcc catgatttct tttacgattt ggcgcatgat tgacgcattt
     2401 gttttaacta aatctaaacg agattgacca ggtttttggt tgataccagc tgtgataaca
     2461 acgatatcag catctttaca ttcttcatag ccacctgacc atacagccac attttcatcg
     2521 ccccaagcca taccatctaa aaggtccaat gcttcccctt ctgctttttc ttgattcaca
     2581 tcgatcaata ctaattcatt agagattcct tggttgatca ttgcataagc gatacttgtt
     2641 ccaacaaaac ctgtaccgac gattactact ttacgacttg tttttttcat gaataaacag
     2701 ctccttttca taactatttg ttactttcat gaaaagtata gtcttgttta ttcattttat
     2761 caacatgtta attataatag ttgttattat aatttttgat ggaaacgaat tcctgaatac
     2821 gcataacaga taaatgacaa atgtttacac ctatatcccc tgataataca accaaaagtt
     2881 tagaactgaa aataaatcca aaaaaattcg ctgcaacgat ataataagac agcaatcgca
     2941 cctaaaacaa ttttccttca tatgctttag tgaaagattt tcttcttaat gtttatttct
     3001 ccattcattg ggagtacatg ttgaatactt cttgaataat ttgaataaag aagtcgtttg
     3061 tttatatccg atacgattag cgatttcttc caacgaaaaa ctcgtttctt gaatcagttc
     3121 caacgcaaag tcgaattttt tctttagaac cagttcttga aaggaagcat caaaatacat
     3181 tttcaattta ttactaagat agaccggatt gtagccaaaa tgagctgctg tttcttttaa
     3241 cgtacaagaa gtaaaatgct tatcgatata atctaatact ctaagcatct cattttctgc
     3301 gcatccatct gctgatgaaa agctttgttg ttctaataaa tgagtcaatt cgatcagtaa
     3361 tagtgatgta tagagattta acgaacggat cttatgactg tatgtatcaa aataaatcaa
     3421 cagcatattt tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa aatgaatgaa
     3481 ttgtgtatgt tccccttttt cttttgatgc atttatcaaa aaagaagaaa gtagattatt
     3541 tgtgcttgcc aaatgattca atatctctgt agataaggtt tcttctttta ataagatgtt
     3601 gattaaaata tcctcttgcc ctgtgtattc gattctttga gtagcttctt tatcaatgat
     3661 caccacgtca ctttcttcca tctctatcct ttctccatta ataatttgta aacatgtacc
     3721 gctatacata taattgattt caacaaaatc atgtgtatgc tcgggaactt ccgcatatcg
     3781 acggtgttta ctgatatgaa tatttccctg ttgaaataga tcccctcggt ggatttttgg
     3841 cggtgtctga agatggtaag ctaagtttaa atcattgata ttcattcctt gttcacgctg
     3901 cagagtttcg attgattctt cgttacgcaa aaaaatgtct aattcagcta gttccaattg
     3961 actccctcct ttttgtaagc ggtttctttc gcttattagt tcacgctgta ctgctattat
     4021 atgcaaagct atttttggtt tcataaagac tttctatggt tgggacatga acttcctgtg
     4081 attccgctat tgagagaata accacttcat actatatttt tgataagcgc ttacaaaaat
     4141 gaaaggaaca tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc agtctctctt
     4201 attttgactt ctgctggtgc agtcacgtct gcaattccca gtatgtcagc tacttatgcc
     4261 acgcgttctt tatctgctgt tgaattatta acgactgctc cctcttttat ggtcactatt
     4321 tttgttttga ttagtagttt tattgctagg aaaatcggac aaaaacaaac tgtgatcatc
     4381 ggattagtga ttgcatcgat ttgtggtatc ctccccctct tctcacaaag ttacagtatt
     4441 gtcttagctt caagagctgg actaggtatt ggctttggat tgatcaattc tttggcagtc
     4501 agcatgattg ctgcctttta tcacggagat gaacgagcct ctatgattgg ttttcaaagt
     4561 gcatttcaag gatttggtgc agctttaatg acatttgttg caggacagct agtacagttc
     4621 ggttggcaat ataccttttt agtctacgcc atcagcttac ctattcttgt cttgtttgtt
     4681 ttgtttgttc ctacacctga aacaactgaa cttcaatcgc atggcacagc cccaacaaga
     4741 caagtaatca aaacgaatcc taaaatgatt agttatggta tttttctctt gctggtcatc
     4801 gtggtatata atgctgtatc gattaaatta gccacagtcc tgacgactta ttctctagga
     4861 aacgaaacta atgcagcgaa tatcttaaca atcatgcaat tggcaagtat ggctgccgga
     4921 gtttgttttg gctggcttca gacaaaagca catcgctata tgcttacact ttctttacta
     4981 ctgatgggaa tcggctttgt attgattgcc actgtgataa acctgtatgt gattgggttt
     5041 ggtgcgatcc taacaggaat ttctttttcg ttattcattc cttacttatt caaccaagta
     5101 agtatccaag cgcccaagta cgcggagagt ttcaatactt caatcttatt ggttggcgct
     5161 aatttaggta ctttcattgc accatatggt ttgctctttc tctcttttgc ttctcttggg
     5221 agtaaaacac ttgctgtttt cgtcaatggc agtatcttgc tgttgattag cgcgcttatt
     5281 agccttctag tagtacacag gacgaataga aagacagtga taagagtaga tgaagaaata
     5341 aaagaacaat tgacaacaaa ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa
     5401 cataaagata gatggctttg gtatcctgga gactttgaga tccgccatgg actgctacaa
     5461 aatttccaac gggaagaacg aggttttgac tggccagcgt actggtacat ggatgattgc
     5521 catcggaacg tcaagtttaa gagatattac tttcttgatc aaccttccat gtttaaagtg
     5581 acgatccaag gagttgggta tgtagaaatt aatggacaaa aacatccatg tggaaaatgg
     5641 ctgacttgtc cagctggtaa agcaaaaatt cgtatctttg taggacatac atcagggcta
     5701 ccagcgatgt ttatcgaggg agacgaagta aaatcagata ttggctggac agcaagtaat
     5761 tttattgaag aatatccagc tggctggagt ccgctttatg tcgatatcgc aaaagatcca
     5821 aatcaaatct attatcaaaa agaatgcgtc catccagtca ctgaaacgga gattggcgga
     5881 ggaatcttat tcgattttgg gcgagcagtg aatggctgtg tagaactaat atctctggat
     5941 gaccatatcg aatctatcac tctatgttat ggagagtccg atgtagaagc tatggatatc
     6001 gattattgct actataagca aataggaatg acaagaaaaa tgaaagctcg taaacgcgct
     6061 tttcgctatt tgtttatacc agaggtttct actggaatgg ttcaacttca agtttttcat
     6121 gaatttttat ctctttcgaa acaagctgct ttttcaagcg attcttcttt actgaatcag
     6181 atctggtcag tatcagaaga agccttcaag ttatgtagtg atttgttttt tatcgacggt
     6241 atcaaacgag atcgctggat ttggtctgga gatgcgtatc aatcattcat gatcaatcaa
     6301 tatctttttt ttgacgagga aatcaataaa cgaacgatat tagcattaag aggacaaaat
     6361 gaaatcaagc agcatatgaa tactatcgtt gactattcga tcttgtggtt gattggtatc
     6421 gaaaaccact atatgatgac gaaagactat gaatttttac gcatcgtcta ccctaagatg
     6481 caatcattaa tgagatactt gatggagcaa acgaatgaat tagggtttat ttatgggaga
     6541 gcaaaagatt ggatatttat tgattggtct gaaatggata aagaaggaac gcttgctgca
     6601 gaacaagtct tattactgaa agcctatcat tccatgatca cttgcggaga tatcttagct
     6661 gaaacatatg gagagaacga aaattatcat actcatctct atcaagaaaa atatcaacta
     6721 ttgaaacaaa acctatttga ctacttttgg gatgaagaac ttggcgcttt tatcgattgc
     6781 tatgaatctg ggagaagaaa tgtgacgcgt catgcgaata ttttcgctgt tttattcgat
     6841 tttgctaaca aagaccaaca gcaatcgatc ttaaagaatg tcttattgaa tgacactatc
     6901 acacagatta ctactccata tttcaaattc tatgaacaag atgctttatg caaactcgga
     6961 cagacgaata tcgtctacca aacgattctt gattattggg gagggatgtt atctcatgat
     7021 gcggtaactt tctgggaaga atacgaccct actcaaacag gaaatgaaaa gtacagtatg
     7081 tatggagatc cttatggaaa aagcttatgc catgcatggg gagcaagccc aatctatctt
     7141 ttaggacgct attttatcgg tttgtatcca acaaagccag gttatgaaga atttatgata
     7201 caacctaatc tagattcatt tgaaacactg aattgtacgc tacctatcaa agatggaaag
     7261 atttcgcttg aaaaaacaaa agataagatc gtgattactt ctgatagaac aggcggaaca
     7321 gtgaaaatga atggtaaaag ttatcctctt ctagcaaaac agccacttgt cttaacaagc
     7381 tattcaaaaa taaatgcata aaacaaaaaa ctgcttgcga catatcagaa gactttaaat
     7441 cttcttctga tattgtccaa gcaattttta ttttgaagtt tgcttaattc tgttctttag
     7501 aaacaatgtt tgttcttttt ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga
     7561 aagcgatgat tccaactgtc aaggctattt gaccgtattc tcctagtttc ccaagtacaa
     7621 gtcctggtac tagataatca gtatcggcaa atgtagaaga agcgatttta agatctccta
     7681 atactggaag gataaataat ggcagccaac taatcaataa gctattgaaa gcagaaccta
     7741 ataccgcccc tcttctaccg ccagtcgagt tgccgaagac accagaagca gctccgcaga
     7801 agaaatgtcc tactacccca ggaatgataa ctgtagtttg caagcctatc atagcaaaca
     7861 tggatagtgt tcctacaaag aagctaacaa aaaagccaat taatacagca ttaggtgcat
     7921 atggaaaaac gataggaaca tctaatgctg gtttagaatc gggtacgagt tttttagcaa
     7981 tcccttggaa tgctggaacg atttctgcca acaccattcg aaccccttga aggacgataa
     8041 caaacccagc tgtgaatgtt ccagcttgga tcagtgcaaa gattacagcg ttgtctccat
     8101 tgcttagttc tctttcaaca aatccaggac cagctaacaa agcaagtgcg acatatacga
     8161 tcgacatcaa gagcatgatc gatacagtag aatctcgcaa aaagctgaaa cttttaggaa
     8221 tctttatgtc ctctgtagaa cggtctttgt ttccaaaaaa ctttccgatc catccactag
     8281 aagcataacc gatatttccg gtatgcccta acgcaacttg attgtttcca gtgatgttct
     8341 tcataaacgg ttgacaaaga gcaggcgtaa tagttaatag tgttccttcg aagaccccgc
     8401 ctaataagat catccacaaa tttgcagtaa accccgcgct tactaaaatg actgctgtta
     8461 gacaagaaac ataaagcatt gcttgacctg taagaaaaat gaatttatat cttgtgaatc
     8521 tagcaagtaa aatattcacg atcatcccta ctagcatgat caaagcagta ggtgtaccgt
     8581 attgttgcaa agcaatcgcc actactgctt cattgctagg aactactcct tgagtatgca
     8641 gagctgtttg gaacatcgaa gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc
     8701 cgatcagtac taggaatccg acaaatgttt tgatagttcc ttggatcact tctgaaaatt
     8761 tcttcttctg aagaacgaga ccaagcagtg caatcaaaga aacgaggatg gagggctgac
     8821 tgacgattga cacaaacaaa ttcaacaatt tatccattga tagttcctcc cttgaatcat
     8881 ttttattctt ttgacaagat cttcgttact ttttctttta cttcttcgct gtcaatgatc
     8941 gattgcaaag gtacgagctt ttcatcaggt atagaagtta gcgtatcttt caaagaatgc
     9001 tcaatgaaga aataatcagc catatcgcta gatacacttc caacatccgt atgacttact
     9061 tcaaactcgt ccacatctgc tccaagatct gcaagtgctt gtttgatgtt catctctacc
     9121 ataaaacttg ttcctaatcc actttgacat actgcaaaaa ttttcatatc attcgtctcc
     9181 ttttcttatg atcgctaata tttcttcttt tgttgatgcg tgtaataaat cttgaagatt
     9241 ttgtttatta gaaagtagct ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc
     9301 gatagcagct aaacagatga acaaactaat gggatgggct ggattatcaa gcagatccac
     9361 aggttgttga agtttcagca aagacatccc tacttcgttt acgccatctt ctggacgagc
     9421 atgaggtaat gcgatgtgat cgccaatatg gataaacgca ccatactgct ccacgcgctc
     9481 gatcatagct ttgatataac tttcttctat tttattttct gataatagcg gctgtgcggc
     9541 aaatgtgatt gcttcttgcc agttttttgg ttgatccgat aattggatca tttcttctgt
     9601 taataattca gatagcatag gacgttgatc ctccttcttt tctaaaagtt tgttcatttt
     9661 acgatttaat acttgataga gcttttcttc gtctacgcct tcttttaatt cgatataagg
     9721 cagtagcgcc gacaacaatt cttgggcaga aggcattgaa aatcctggta tcagccaatc
     9781 tctttgtact tgattgatca gctgattttt ttccaagtca gtcatgattg gatgcagaac
     9841 atagagagac tttttcttat cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta
     9901 agaagtttcg tcaacctcgt gtaagcggtc agctgaatta gtctctgtaa aaaagatcat
     9961 cgggaataat tgctgaagtt cactttttaa gatcaatgaa gagctaatgc cacttggaca
    10021 gacgatcacg gctcttattt ttctatttct ttcttctgcg tccgcttttc tgatctctcc
    10081 gccaaatagt atagtgaaaa aaccaatctc ttcttcaggg atgctcttcc ctattcgtct
    10141 ttctaacgga agtaatgctt ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat
    10201 ttgatcgatc atcacattag gtaaataaaa agaataggaa atacgaaaat aggcagggac
    10261 caagtgataa aagacattca tcaataattc acgaaacgaa gcaaattcta cggccatcag
    10321 gaattccatg cgatggatga cttctgaaga acattccagc aaaaaatcaa gtgcaggctc
    10381 ctggacatct ccttcaacga tcgatgctaa gcaaagagag aaaaatataa tctccgaatt
    10441 ttgatgttcg gaatagccaa ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag
    10501 agaagccaac agttcagcat cttttaccag ttccatggat gtttttccaa ttcgaatggt
    10561 tgtcaaactc aaataataag ctaattcatc cattcgattt ggtgcgaatc gaagatctga
    10621 tagtgaaatc gttttatcta gcaaatctct aactttggcg taataatcaa tggaaagggt
    10681 tgcggttaaa tacgtgagcc caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg
    10741 attttttgct tctcttctta atattgcttc tgaccctagc agcctaaaac cagttttcct
    10801 agtatacgac agctgacaat cttccacttc taattcattt cttatctttt tgatatctga
    10861 caggactgtc cctttagaaa cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt
    10921 tgcagtcgaa taaaccatca aataaagaag atctcttcgc tcttgttcgg tatacacagc
    10981 ttcaaatcga gtaatatcca aaaatttttc tgcccagtcg attgctcgga atttctttat
    11041 acggatacgg gtatcatcca catattcggc aaaaggagtt aattgtttga tcgcctcgct
    11101 tgtatttact tcgatgatct ctgctgcttt ttctagactg atttctcttg gtgttttcag
    11161 gagataacta attttgtagt tcacagcgct gttcacctcc tacttatatt ctagtatggt
    11221 agcgctttta aatcgagggg tgtttggtac aacttacagt tggatttttt ttacgactgt
    11281 accgaacgat ttctatcgaa tggatacaga aaaaaagcag gtgaccaaat gtctcctgct
    11341 tttatgaatt gatttcgtcc gatcaatgat gatcatgttt attttttgag gtcagttctt
    11401 caaatttttc tttgacatct tcaaccgctt tttcgatatc tttttcaaag tgattgggct
    11461 cattaggctt tgtttcctct tctacagaat ttgaaccaga taaagctgtt tctttatttt
    11521 ctgtttcacc agagtttgtg ccagctaatg attcttcttt gtcttctgtt tcaccagaat
    11581 tagttcctgc taaagccact tcttcttctg ttttaggggc cattgaacct cttaatcgtt
    11641 tgtgtttttc accgtcataa tttgtcatgt tggttcctcc tagtagttct atactttttc
    11701 atcgtacaat aagacagttt atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat
    11761 atttttcctt ctgattcttg aatgcttttg attccttctt ccaaaatttt catttgtaag
    11821 attgtttctt catctttgat gactttttct gcgccattaa ctaatgtctc gtaaatccct
    11881 tcgtagacac ggctgtaatc accaacctcg gaaattactt tttcttcatg ataactccct
    11941 tgttcatcta tataggtcaa tacaccataa tgttcgggca aatcgatacc aaagtcttca
    12001 tgatcaggca tgtaaaacat tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg
    12061 aacatccctt tttttccgta aacgacgaaa cttggacgtt ctttgatgcg gaaataactg
    12121 gattttatcg aaattttcat atttccatag aaaaaatcca gatcgaagta atcattcatt
    12181 cgtcctttcc ctaaaagttg acgtacatca tagctgatat catctggttc gccgaaataa
    12241 gaaagaactt gatctactgt atgacaagca tgtccatata aatagctgtt cgcagtagaa
    12301 aattcatgcg tattttctgg tacttccggt ctgaaataat cataatgcat ttctacttct
    12361 aaaatatccc ctaattttcc gctttcgatt actttttgga ctgtcaagaa gtcagaatcg
    12421 aaccgtctgt tttggtaaca ttggataaat agaccttttt cttttgctaa tgcaaataac
    12481 tcttctgctt cctttgacgt ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt
    12541 tcgagtgcca tttttccata ttcataatga aattgactag gtgtagtgat gacaactaat
    12601 tggatctcag gatcatccca aagatcagac agctgatcag tatattcgat gtcttcgaat
    12661 gttttccaca ctggttcaga tggggaaaaa atttttttga tattgatttt gttcgtaagt
    12721 tttcttgaaa aaggcaaatg atagcgattt gtactctttc cattgccaag ataagcgatt
    12781 gtaagcatga gtggtgcctc cttttattga tgttttcagt atatcctaga aaagcgcaga
    12841 aagtatttat aatagctatt ctggtaaaaa agttcagaaa aacacttgtt atcattcggt
    12901 ttggtgaaat taatgttcaa agtaaggata aaaatcaaaa agatagtagc ggaaccaagt
    12961 gtttatcagt aagatagaat ttggaggtca gatcatgctt attgaagaaa aactaacaaa
    13021 acaagagttg tttaccacga cagagaaacg gattgtcgat tacatccgta ggaatatcga
    13081 agctgcagtc tacatgacga ttgaagaatt agcaaaagct acgtatactt cccattcagc
    13141 tatcattcgt ttatgcaaaa aaatgggatt cagcggattt aaagaattcc gtttcgagct
    13201 ggggcgggaa gttcatcaat tgattgctca gttcaatcaa acgattgatc ctaattttcc
    13261 tttttcaagt gaagatagca ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca
    13321 gtcaataaaa aaagcacaat tgcagattga acatgaagat ttagatcaaa tcggtaaagt
    13381 attgaacaag gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa tcacggcaag
    13441 gaaattccag aataaaatgg tcaagttgaa taagtttttg atcatggctg aagaatacag
    13501 tgattcttct tggaatgcag ccaatttgac tagcgaggat tgcgccattt ttattagcta
    13561 cagcggtcgc atccatcatt atgaacgaat aatgacctat ttgaagcatg tagaggcccc
    13621 taccttgctg attaccggaa atcagcattc agaaatggcc aaacaggctg atatgtgtct
    13681 tgttatatcg caggaggaat atgattttgc taaagtagcg actttctcct ctcaaattgc
    13741 cttcgattac gtattaaata cgttattctc agtgatatat tctcaaaatt ttgaagagaa
    13801 tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta aagaatatcg
    13861 gaaaacttaa gaaaagatcc tcatttagta ataagaaaga tagtgtttga taggctctct
    13921 ttgatatcat cataaaaaca ggctgaagtt ttctttttca aacctcagcc tgtttgttta
    13981 cggaacgatt cgaatagata tattatagaa atgaggaata gctaacttct gtttcttatt
    14041 ttgaacgatt gacgaaaaag atatataaga aggaggaaaa tcagtataca tgcagctaac
    14101 aatccataca ggaaaaatcg tttgttctcg gatactttct gtacttcttt ttgaacttct
    14161 tttgcatgat aaggaacccg gtgccctctg acaagtaagc gatgactgtt gaccatataa
    14221 ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt cgattctcag atcttttgta
    14281 tcagtcggtt ctaccgtctt gatctgatcg acttgataag cgagtgtccg tccattgatt
    14341 tcgatataga aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga
    14401 ggcagtcctc gatggctgga aataacggca tgtgtatttt ctccccccgt aggataagac
    14461 gttccttcca ataatgaggc acctttttct agcagcacag tattcgtttc atcaaaaatt
    14521 ggcaaatgga cattgatctc agggatcgtc aaaataccga tcgtatgttt ctcaaaatag
    14581 gttctatcct ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc tggatttcca
    14641 ccttcttttg ctaattgctg gtttttcttt gtcatgtttt cttggatttt agccatgact
    14701 tcttcatttt ctttgaccgc ttgttgctga taatgagaga tgatctgctg atccagataa
    14761 ttattcagcg cgtcgctaac aaatggataa gctagtatcc ctattcctga gagaagcaag
    14821 aaaagcatca gcagatctag aagtttcctt ttctttggtt tcatttgtgt tctccttgtg
    14881 atgactgttt gataataaaa gaaaaaggag catttgcgta atccgctgct cctttttcca
    14941 attcttgcaa aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat
    15001 cagtagaagg actactcctg caccgatata cacatagatt cccttaccgc ctgttgaagg
    15061 aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc ctgctgttac
    15121 ataagattgt tcatcgatag taaatgctac acggtttgtt aatggaacat atttttctgg
    15181 cgctttcgtt tcttccagat agtacgtgcc atattttaga cctgtcacat cgattaaacc
    15241 gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc tgacggcttt
    15301 tgtggatggg tcgatcgata aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa
    15361 ttctgctcca gcaagtgttt ggtctgatgt aacgtcacca tctactttaa caaatcgttt
    15421 gcccccagta acgacatcga ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac
    15481 atttgcttgg ttagaaaatc ctttggttgg atctgctttt tcgttcaaat gcatatagta
    15541 aacgaatttc aatgtaccgc caggagttaa tgaaggaata taattcggat caactgaaac
    15601 cgtgaatccg tctgtttgct cagtgacaga ataatttact gggtcgattt ctttatttcc
    15661 atcatataag gcataagcat acgttcctga agaatcatta tcaaatgtta aagcagcgtc
    15721 atgagtatcg ataagtttga atgttgtgta cttgttttgc gttccttctt tatcagcgat
    15781 acctaatggg atattgacag aaatctcata ttgaattttt tcaccgattg cgacatcttt
    15841 tccattcaat tgaggtgttg ttttatcaac tttagacgta tcatttttga tggtcttttc
    15901 tactggtgtt tggctatttg ccaggatctc aaaaggcgtg attgtttgct tttcaatcat
    15961 ttccgcatta tcaggagctt ttacttcttc taaattatat tttccaactt ctaaatgatt
    16021 aacgatcaac tggccttttt caatagatgc ctcggcaaag tcattgttgc cgatgtcata
    16081 agaatgacca gtaatgaaat gtttggcttt ggtttgatca gttgtccaag tgtacaatcc
    16141 atctgtgaca ctttggatgt attttttgac gcttggtgtt ccttcttctt tagaaataat
    16201 aaattctgct ccatttagtg cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt
    16261 tccatcatta ccgactgtat ttttagggta gagatggata gtatctagtt cttctgtccc
    16321 gtatttataa gagccatctg cttgtttgat catctcatat acagggaaag ctaaaaccat
    16381 gtttgcggca gctgacactc cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt
    16441 cccattttgt tttttaggta aagataaagt gacatttcca tcagcatctg tcgttcctga
    16501 agcaactggt gtaccaggag tcaaagactg gactgcttgt tttgctgcat ccacggacgc
    16561 tcctttatct cgttgcgcat aaaattcttg agtgacgtta taaactgaaa atgaaatatc
    16621 ggctaatcct tggtattgat cgaattcgct catttctttc ccgctgtttt ggattaaagg
    16681 atcgggtaaa tcagtcattt tctttttatg aaggatgact tgagctgctg tctcctctgc
    16741 aaacactttt tttgcgccga agctgtttgt gagtaatggt aaaataagga aaaggactcc
    16801 taacataacg tttatttttt tatgattttt cattttttca ctcctgttct ttttttgata
    16861 aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc
    16921 gtttcgtttg tttttggaag aaatggacgg ctattttctt ttttattgac tactttcaat
    16981 atactgtccg cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt
    17041 tcacttaaaa tataaccaga aggtgcttcg atctctttta atcgataact tcctgttttc
    17101 aacccggaaa ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc atctgattcg
    17161 ttctttgtcc acttgtatcc gttggctgtt tgctggaggt aatttcctgc tttgttttta
    17221 ataaggaata ctgcgccagc taatcgttta tctggtttat ttgtctcgat tttgacaaat
    17281 tgcttactcc ctgtatatac agagactttc tttgtactga caccaaaacc aaaatccagt
    17341 gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg catctgataa
    17401 gaaatggtca gtttttttcc agcatatttt tccaacgaag ggatcgaaaa taaaacatca
    17461 aatccgtgag gtttcttgtg tatgacaaag ccttctgtca gcttttctcc attcgatgaa
    17521 atcgttaaac tattaggtaa aaacgttaat gcttcatccg cactatctga caattcgaac
    17581 ttttgatagt caaggatatt tacaggaatt gtcgtatgca aggaataagt gattttttca
    17641 ccaatcgtaa aatcatttct aggctcttct aataccttct caaaaggtgg atcagggtag
    17701 tcgttttctt catttttagg atacaaatgg atagttgaaa gtttttgatt gttttgtcca
    17761 tgtacaggaa gaacaactac catattctct gccttttctt tgacgacttc tggtactttg
    17821 gattcaatga aaagatacgc tttatctctt ttttcttgat ctttagcggc cagagaaaaa
    17881 gaagcgatac catcttcgtt gttaagagtt gtagttgttg cttctgcggt aaacataccg
    17941 gaagacgcac cgttttttgc gatctctgct tgtgcttctt ctaccgtttt gcccttttcc
    18001 cgtagatggt aaaaagaatc tgtgacatca taaacttgga atgtgacacc atttaatcct
    18061 cgatacgttt gtaataaagc tttttcttgt ccgtcatttg gatgattttt cggcagttgc
    18121 ccgtttggga aaagcaattt gtgtaaagta atctgaacca tttctgtttc tgtttctgtt
    18181 gctacctgag taaaagctgg tttaaatagt aacaagaaga gacacatact aagccaacca
    18241 agttttttca tatttagctg ccccctcttt cttttttcaa aaaaagatac caaatagaaa
    18301 acgcacaacc aatcatccct actagataga ttcctaaacg gccaattcct cccgtttcag
    18361 gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg
    18421 gtgacagaac agattcatga tcttgtccat ccacttgaat tgacccatct tcgtgtataa
    18481 ctatagtaac tggctctttt agaccttggt atccttctgg tgtaaaagtt tcggtcagcg
    18541 tataagttcc aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg
    18601 gcgattctaa gtccatttct ggtccctgca gtctgaattt tgctcctttt aatgtctgtc
    18661 cgttgtcttc ttttttgtgt accgttagat caaacgcttt tagtcgattt tgatgagtga
    18721 gcgtccatac attatttgtc atctcggtat tttttcccgt gtaaatgatt tttccatatt
    18781 tatcgatttg gaattcataa tttcccggcg aagtatcata tccggcagga ccagctgttt
    18841 caacgactcg atatttacca ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg
    18901 ttgtttggct gtcaattggc tgataagtac catttgcttc ttttttctgt agggaaaatg
    18961 ctgctccttt aagatttatc tctttgtccg tcccttgcat cgcatactta cgtactgcta
    19021 caggaacttc aacaaaagga ttttcaatag tcaactggat cgtatcatcg gaagtagtga
    19081 ctgtttttcc atcaatggtt accgtaccat cagaagcgat cttgatttcc caagtagtct
    19141 ttttgcttaa tccatgccct tctggagctt ttgtttctgt cagcgtatag gtcatttctt
    19201 tttgcaattt gacattttct ggaagagaat aggttccgtc gccatgatct gttagtaaag
    19261 tatcaataga atctcctgtt aatttgaaaa cagcgccaat aagatccttt tcacctgtag
    19321 aggaattttt cgttattttc aagtttaacg gtgtgaattg tttagtattt ttccatgtca
    19381 ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac
    19441 tgaatgcttg accttgatta ttgtattgag gtagtgataa tatctcttga taactttctc
    19501 cgctatttgc agataatttg tcaatgtctg cacgttccca cgtatttgtt gtatctttag
    19561 ctggtttaat gattcgaata tatccgtttt tccaagctgc agcatttgtc gtatgttccc
    19621 gttgaatctc aaaagtaact tgatctggac gatcagctag attgttgtca aattcttccc
    19681 ataacttttt gatgtgaaga ctgactccgg gagctttagc agatggtata ccgaattcag
    19741 ctaattcatt ggtatcaatg tttggctgga aagttgtcct gccgttcatt tgataccaga
    19801 aatttggatg gaagtcctca ttttctgttt ggattctcac ttggtaatgg atttggattt
    19861 cttggtcttt tcctaaatag atctgattgc tcttgatggt atttccttct atggaaatag
    19921 atggagtgac cgttgtagga cttgtcccca cactcttgac tgaaagtgta ccaggctgat
    19981 aaatgaatgg ttctgcgatt ggatcattta tttgtccatt gcttacagtt gctgagatct
    20041 gtacagcttt tttggctagg tattcagaga tatctgttgc atgatcagct gattcatagt
    20101 aaagatcgcc tttttcatct gatgaaacca tttgtcgcat acgagactct acttctgctt
    20161 ttgagagacc agctgccgga tcgctttgaa gttggatgcc aagaccatgt atttcgattc
    20221 ctcgttcttt gagtgccatc gcttctccga tggttgcgat aaacgtactg tcgattcttc
    20281 tggatagatt gttttggtca ggtgcatcat agattcttga gattagagaa gtatttcccg
    20341 gccgatcttg cgtattagaa aactgagttc cgtaataatt gctgcttgat tgtgcgtgta
    20401 cccgctgtac tttataggaa aatgttggta caccatccgt cagcaaaacg atcacttttt
    20461 tatgtccatt tggaacggat agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag
    20521 taccaccatt tgtccgtgaa ggtgtaatgg attttacttg atttttcact gaatcaaatg
    20581 aacccatctg tactgcaccg ttactgtagc tatatccttc gcttgagtag ccgacatatc
    20641 ccatattgat tttgtctgtg ataccgctat ctgctaaagt atcgacaaaa cgatcgacac
    20701 caatctttac ttcaccgatc cgattattgt cgttcatact tcctgaccag tctacgacca
    20761 atacgagatc aagaggcgtg atatcttttt gtacatttcc acgagcattc aaataaacat
    20821 caaacaatcc gggtgtactt gtttcctttg catactttcg tatcgcatag tctgcttgat
    20881 taccggttcc tccatattcg atatacgaat gggtccgatc attaggatct ccattccaag
    20941 aatttatacc atcccattga tttgttccgt ttttattgcc ttgatgatca agaacatttg
    21001 tatttcctgt aggttgccaa ctggctttag gataagtgcc ctgatcatct gttgtatatt
    21061 ctgtaggaat cgtttgatta gatggcacac tcacagctgg caaggataat gtaggcaatg
    21121 taaatgggct gacagaattt tcgttttctt ttgatgattc ttcttctatt tcgtctggct
    21181 ttggtactga cacatctgta tctttcttat tttctgtctc atcaataatg tcatattctt
    21241 tattatcgac gatcagcttg tttgcctggc tgtggtaaag tgctggatcg attggaaaac
    21301 tcagatcgat agtttgttta gtgtttgatg agatttccaa cgtaattttc ccgctattta
    21361 cctgataagc atagccatct ccttcttttc tttccacatc acttaagtaa ttttcgacta
    21421 gaccaatttc tttttctatc ttttcagcac tggtgttatt cgcatttagc ttgatgttca
    21481 gtgctttttt tccagactga tccgaaatga cttcagctga aattgtttct aaaactgttg
    21541 gatcatctgt agtgtctgct attgcagata atggcacaaa gtttgataaa ctcaatatta
    21601 tcagcatgaa aataactttc agtttcttcc ctgtggtcgt catttttccc ctcctttttc
    21661 tttttttctg ttttgacgtg aatagtgaaa attattaaaa taaacaggcg ttacataatt
    21721 ttatttattt taatatgata attattatac gaagggcgct aaagggaatc acaccaattt
    21781 tcataccaat tggtttcatt tatgaagttg attccttcga ttaatgagcg tttcattgaa
    21841 agaaaagggt tgagggagaa aaacatatga tcaggttgtt ggaaggaaac tatagaaaga
    21901 tggaaaaaat cattcgaatt ctgtccattt ctgacaagtg gatgactaaa aaagaacttg
    21961 ccaaacgaat tggtagttca gaatctacat ttgtaaggta tatcgaagag atcaaaatcc
    22021 gctggggaac ggatgtaacg attcaatctt cacaaaaatt ggggtatcgt cttgaacatt
    22081 tgaatgtacc agcttattta aatgttctga tggaaatgtc gcaaagttct acaaacagtc
    22141 gtttattaaa tgagattatt gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa
    22201 tttccatcag ccggtcttct tttacacgta agttgaaaca atgcaatcaa attttagaaa
    22261 gctatttatt aaaagtgatc gttgaccaag gttttcagtt aactagcctt aaaagtgagc
    22321 tccagttgcg tatctttgtt acctttttct ttttaagtta ctacggaaga cgcacgttgc
    22381 catttccaat cgaaaaaaag aagatcaaag aaattatggt caagaataat tgtcagatga
    22441 atttgtttca ggaagaaagc ccatatgagc aatctttctt tattatgtat tttgttgtgc
    22501 atctcatgag agaatcccaa ggaactccat ttgaatggaa agctggagac tcttatgtac
    22561 agttgataaa tatcgataaa aaagacttct taatcttgaa aaatctctta ggaaaattga
    22621 agtacagcag ctatataaaa gtagtgaact attttgtcaa tacgattttt tcagaatttt
    22681 cggagaaaga aaaaaatgaa atcagtgagc agatacgaaa aaatatgtcc tactgttcgt
    22741 tttcatccgc tgattgcaaa gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa
    22801 tttatttttt ctcccgtatt ttcccatacg atacgactca tttgacgatc cgtgcaactt
    22861 actttgcaca gcagatgcaa caccgccaga tacgtctata tgctggattt cgtgcagaac
    22921 ttcaatttta ttccactcta ctgaatcaga atctgattta ttatttatct agctatatct
    22981 tttggatgct tatcggtaat cctgaaattc accattatac ttctgataaa aaaatactga
    23041 ttatcagcga cttaagtctt cgacatagtc aatatattga agaatatatc agtgatattc
    23101 ttgctgtgca taaaattcac tctgagacaa ctgctattac tgaagatcag ctgtcgaaat
    23161 acaatcttct cgagtatgat ctgatcgtta cgaatcaacc cattttgaat gcccatgttc
    23221 ctcatatatt gattgatgat agtgtgtcat tcgcaaatga agaagaactc atccgcttat
    23281 tcgagttatg aataatcaaa aaacagctat gtataaacca ccctgataaa aatttcagga
    23341 caaatacaca tagctgtttc tttttattgt attccttcag caatccaacg agatttatag
    23401 cgaaagtctt ttaacaactc ttcttttgtc caattatttg caatgagatc cgtcaaagtg
    23461 ttcattacga gggtcatgga aaagtatagg aaactaactg cttcttccga cgattgaatg
    23521 gagaaatgat tgattcttaa taattccagc catttttcgt tcattggaga atcagccgat
    23581 agttctctac gtttggttgt ctgctgatca tttcgctggg ttaacaagta taacctttgc
    23641 caatagatac tttcgtgatc taagtcgcag caccactcta aatatttttc gatccccaaa
    23701 aagaaatgtt gtttgttttc gctaatgaat tttaagatat cttgatggac tatgtgagaa
    23761 ctgcttttga tcgtatacaa ataagcatct tccaaatctt gaaaatattt ataaaaagca
    23821 ccgcgcgaca tccccatctt gtcaacgatt tctgagactt tgacttggct gatgtgctgt
    23881 gagcaaaata tctccagtaa taggtcatcg attctctgtt tctttgcgtt cgataaattg
    23941 aaaaaagttt cttttggcat ccacatctct cctttatctc tgattatacg tggatagccc
    24001 ttcattgtca tgaaggattc attccttctt tttctcttct tctgtcaagt acccatcttc
    24061 cattcgataa acgcgatcac tccattgaat catacggaca tcatgagtaa ccataatagt
    24121 cgctttttgt ttctcatgag cttctttaac tagtaatttg acgacttcat atgcatgatc
    24181 tgtatccaaa ctagctgttg gttcgtctgc taagatcaag cttggctcat tatataaagc
    24241 gcgagcaatt gccactctct gtctttctcc accagaaagg tctttaggat aactattttt
    24301 aagtgactgg atatctaaag attgcagcaa ggaatcgatt tttgttgaat cttctttttt
    24361 tgaagtaact tggtcaacta gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt
    24421 agagctttgg agaataaatc ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg
    24481 aagctcagtt agtgagtgac cgttgatcac tatttctccg gaagtcggtg tttgcaatcc
    24541 tcctgaaatg gtaaggaaag tacttttacc tgaaccagaa ggaccaatga tgctgacgaa
    24601 ttctccttcc tttacataaa aatcaattgc tttcaaagct gagacctttg tatgaccaga
    24661 accaaatgtt ttggtaactt ttttcatcgt taatattttt ttcattttta ttaacctcct
    24721 attgctgtaa ttgggtctac tttggttatc gtacgtatcg aaaacaatcc gcctagcatt
    24781 gcgacaatga tcaaaatccc actatatagc aaccattgtg accaaaaaac agcaaaaggc
    24841 atcgcgcttg gtaaaatcag actagtcaaa taagcaaaca aaagagcaag cagaacgcct
    24901 accaccccaa caataaagga ctgtgctact aaagatttag cgatgaagaa atttttgatc
    24961 ccttgtgctt tcattactcc aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa
    25021 ataccgacca cggcagcaac cactaagaac aaaaagtata tcatcgcatt taaggtcata
    25081 ttttgggcag aatagccagg aatactttca atgaatgtcc caatagttaa taactgtaga
    25141 cccttcgatg ctttttcttt cgaaatttta gctgaatcct ttattacgat cccattgatc
    25201 ggctgttctt cttcagaagc aaacggctgt tgtccatact tcaaagcagt ccacgtatct
    25261 agggacgcat agatgacggg agaaactgtg taataagttt caggaaagat accgacaatc
    25321 gtcaaaggat cgtcgtaact gccaattttg atcgtatctc caattttata gcctgaatca
    25381 gccagatttt gagagatgat gatttcattc tttgttttaa attcatgccc tttcgtgagt
    25441 tttggtaata aaaatgcttc tttcgttgtg ccgaaaactg taacattttc tttgtttttt
    25501 cctttgattg ctccgctgta caatccgatt ggtgctttct ctcctccttc aatgtatttc
    25561 agatctcctc ttgttaattg tgaagctgca aatacttgat ttgcatcctc cgacaaaaca
    25621 atctcttgtg cgtcccaatc atcgattgct tttttaaact cttctgaaag tccattcgct
    25681 agtcctgaca acatgaatac gacatacgca atcaagaaca taataccgac gatcaagcca
    25741 tatcgtaatt tagaatagcg aatttctttg atagctagaa acatttgttt ttcctcccta
    25801 tgatagattg atagtgacaa aatgtcactt gttgaatagt gtaactctaa agtgacaatt
    25861 tgtcacttgt gttgtttttt tcttatcaat aaattgtcta agacatcttt cgataaaacc
    25921 ttaacatgag tcataaaaaa tttatttaag ccttatttat gctttccatg ataaactaaa
    25981 gtgaccaaat caatgaggtg ggaaaatgat taaactatta gtagtagaag acgataaagt
    26041 attatccaat aatataaaag aaatactcgc tgcaatcggc gagataacac aagtttatga
    26101 tggtagtgaa ggactgttcg agttggaaag tggagtttat gatcttgcaa tcttggattt
    26161 gatgatgccg gaaatgaatg gatatgaagt gttagcaaaa atacggaaag aaaaaataca
    26221 gactccagta ttgattttga ctgcaaaaga tagtttggaa gataagattg aaggatttga
    26281 acgaggtgca gatgattatc taacaaaacc tttctatcga gaagaactga tcatgcgcgt
    26341 gaaagcatta ttgaaacgct cgctaggtct ctttgatgaa aatgttctga agtataagga
    26401 tatcagttgt gatttaaaaa gcaatgaagt cttgtataaa gaacaagttt tgcccataca
    26461 aggaaaagag tttgaattgc tcgtttattt tttacaaaat aagggaatca ttttgacaaa
    26521 agagcagata tttgaccgga tctggggatt tgattctgat acgacgatta cagttattga
    26581 agtttatatg agcaacttac gaaaacatct gcgaagtact ggattggata aagaaatcaa
    26641 aaccttacgt aatgtcggtt atatactgca aagggtgtga tgcaatgaat ggagatatta
    26701 cacgaaagca acgtagtcgc ttttttgcga caaatgtagc agcatttgct tttatctttt
    26761 ttcttttggg attcattact ttacagatat taaaccaatc tgcatacaga gaaacagatc
    26821 tgtcattaga gaaaataaat tttgattctc gaacagtaca attagagata caaagatatc
    26881 agcaaggcga tcccttttta aacccgactc aaacggaaca aaacagaaca aacatggaac
    26941 agccattaat ggaaccatca aatgaccgtt ttaacactca agtgatcttg tggtctaaaa
    27001 atggtgagat attgaataaa gcagctattg gtgggcgttt caaccaaatc aaagaattaa
    27061 aaatggatac gaaaaatctg aataccatcc aaaatattga attaaatgaa gacagtggct
    27121 catctgccct ctctttccgt tcgattacga aaaaagcgga gacgaatcaa gatggcgttg
    27181 cctacatcca aattttagaa aatacaaatc aaatcaagaa ttcattagcg acttttcgca
    27241 cgattatgat tttgtgtatg attgtttttt ggattctctc tattggtatc agttattact
    27301 tgtctagtct aagcatgcgc cctattattc tttcttggag aagacaaaaa gaatttgttg
    27361 agaatgcttc tcatgaattg cgcacaccgt taacgattat tcaaaatagt ttggaacatc
    27421 tttttaccaa gcctaatcat actattattg aagaatccga aagcatcgct caagcattgt
    27481 cagagacgag gcgcctcact ggattgactt cggatttgct gacaatcgct cggaatgatt
    27541 ctgatcagca gctgctttca aagcaaatga tcaatacaca agaatatatc gaacaacttg
    27601 ttaaaccatt tcaagagatg gccatcatag atggaaaaga gtttattctc cataacgatg
    27661 caacattaga agtaccaatt gatgaaaaaa aaatccatca agttttggtt attttgctgg
    27721 ataatgcatt aaaatatacg aatccagaag ataagatcat tttggattca tctgttaacc
    27781 ataaagaatg gctgattcac gtaaagaata ctggcccttc tatcagtgaa gaagatcaag
    27841 caaggatttt tgatcgattt tatcgggaag atcaatctcg ggcgaaagag actggcggat
    27901 acgggcttgg cttggcaatt gcaaaacagg taatcgaaca gcatcatgga cagatttctg
    27961 tatccaatta tcttccacaa ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa
    28021 aataatgctt atccctaaaa aagggctgtg acaaaaatct tgtcacagcc cttttttgtt
    28081 gcgaataaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tctttaagct
    28141 gaccacttct gtcacaacct ctttagctta cgcttacgat tttttattgc ggtcttcaat
    28201 gtgtacatca ccgcttgtgt caatatccaa ttcttctcgg ctaacatctt ctgtcacttt
    28261 ttcaacatct tcatgttctt ctttatgaat cccaacttct tcacgaataa ctgtatgttt
    28321 gcttacatct acttgctctt ctttgattgg gatagtaatc gtgtcatctt ccaatttttc
    28381 gtctgttccc tgtgaagatt gatctgttac tggtttgcgt tcgatgacga tttcttcacg
    28441 tttgaccggt acttctaccg tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc
    28501 tccagttgtc acatcatgtg tatttacatc taatttttct tcttttagtt gaattgtatc
    28561 atcatctaga tttggtttgc gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt
    28621 ctcgccaggt acgtccatcg gatcagttcc tggtgtcata ttcaatggat ccgttgtatt
    28681 ttgaaagcct gtcgtgccac ctacgtccat tggatctgtt ccagtgccag tttctaatcc
    28741 agtccccatt gtgcttgctg ggctaacagt atcttgtgta tccattgtat cttctgttgt
    28801 ttctggctga cgatagcctt ttactgtgat gacaaatttt ccatcagaaa tatctcctct
    28861 atattgatat aaaggatcat tttctggtgt agccgtctca gaatcatagg tatcaaaaga
    28921 aaaagtatct tttatttttt cccataaaga acggtcttct tcaccatcca tccgattttc
    28981 atctgtttca acatctatac cagacaatcc gtcaaatcct agtctagatg ctgcttcatc
    29041 agtagtataa ataacgatat catctcgttc atacccttcg ttacgcaacc gttcaattac
    29101 tgataatgct tcttccctcg tactatagct tccgatgacc gttaaatttt tctcttccat
    29161 gtaaattcct cctagcaaat agatttatga ttggtatatt gttacttata aatttattct
    29221 ataggagacg attgttttct acaaatattt agattggaat ttgattaaaa caatttaatt
    29281 gaaatttgaa aacatttatg ttaatgaaag tcatattcta ataaaatgtc atttgtttct
    29341 atatgaattt ttatgaaaaa tttcatataa tttccactcg ttgtgatggt ttaatttatc
    29401 caatccatca ataagcaaac aacttatata aaaaaggatt aattctcaat ataattgctc
    29461 ctaccataga ataaatcttc atgtaaagta atgaagtacc aactatttct aatacttctt
    29521 taagaattat cccaacaata tcgttagaca aaccattatt gaataaatgg agagccaatc
    29581 gattctgaaa aaatgttttc taatttttcg gaaataaaag aaagagcctc ctgccattag
    29641 aacgaaccaa atccatttca ttgctcatga agtaaccaca tagctaaaaa gaggataaat
    29701 caattaaaag atcaaaagaa tgactgcact aataaaataa ctaaaaatgt atcttcttac
    29761 ttcatatctt ttagctagta cttagttaaa aatttgcttc ctaagatcaa gacaaactgt
    29821 ctatcttcat ttgatgaaat gcggtaaaat atagaaaatg aacactattg gagggaattt
    29881 gtaatggaca gcaaaactag aaaacaactg caagaaaagt taaagcaacg tacacacttg
    29941 ctcccttccg tctttcaaca gtttttagaa gaaaatcgtt cttccctttc attaggcagt
    30001 cgctatgaat acgcaaaaga ttttcatctg tttactgatt atttgagaaa agtattttct
    30061 gaagaagtgg atgatcagct gattatccat ttagaaaaac aagattatca gaatttctta
    30121 gctaaaatct atcgacatac tcgttcgttc caaactacag cgaaccaaga aacggagcaa
    30181 ttgtttgaaa atagctatta cgggatttca aggaagcaat attcattaaa tcatttttta
    30241 cgatttttgt atcaaaaagg actgattgaa gaagagattt ctcttttggg tacaagttta
    30301 ccggaaacaa ccaaagcagc tccgcgatat atcgatgcag aacttttcaa agattttgag
    30361 catttgttta tcccgattga aggctttcaa acaagcagag aagctagctt tgaagaaaag
    30421 aatagaccga ggaaccttgc aattactgcc attcttctgt attgcggatt aaagatccat
    30481 gaagtagtag aacttaaacg taaagatgta tctttgacca agcagattct taatctgaat
    30541 agaaaagaaa acagactgaa taaagtacca ataccaaaag aagcgatgcc atttctggag
    30601 aactatttta agttaacagc acaaaatacg gacgaaaatt cttttgtttt tcgttcctca
    30661 catgatcagc aaatttctcc tcggactatt cgccaaattt tgagcaaaat tacagtatat
    30721 aaaaacgtat cgccagaact atgcagaaaa acatatcgtt attatgcaga actatattta
    30781 gatgatgctg acgcggtaaa ttatttatgt ggtaatcgat acctctcatc ccattctttt
    30841 caagatatct gggaaaagat ggttgatttt tcttatcacg aattagcggc attcgttcgg
    30901 gtttcttcca tataaaacaa atgtatgatc tttttttcac aataaactat gttattatat
    30961 aaaggattat gttacgatta tcttgaattt gtatattact taacaaaaga taatggagtg
    31021 tgaatgatgg agaccataac gtattcccct ttgaacttat ttacgaattt tcaagaagct
    31081 gcagctaact tttccgagac cccgattatc tttgatgagc cactttcagc tttcccagaa
    31141 ttaggacttg aaacaactta caaaaaatgc tgcgaaagtg tgttgaaacg ggcttatcaa
    31201 ttggctcatc ttggagtgaa atccggagac aaagttatta tttataagag ttccgctttt
    31261 gacacatacc ttctagctgt ttctgtgtct taccttggtg cagtgcctgt catgacttct
    31321 tatcatctac caacaacaac aatggaagta tttatcgatc gtttagaaga ccctttcatt
    31381 ttatttgatg atgagacaaa aaagcgagtg agagaaattt caaatggaac aaaatcaaaa
    31441 cagattccta ttctttattt aatggaacaa cctgctttgt ctgtttctca atcattttta
    31501 gacaaaaatg aaatttctta tatgacccat acttctggta caactggtat ccctaaactt
    31561 atttgtcatt ctgctcattc tatgggatgg cggacgaaat ggcaaaaaac agttttcaca
    31621 aaaatttcgg aaaagaaatt aatcgctttt cacatctctc cagtgcattc cagattcaat
    31681 attggtatct cttctttgat gtccatggga tttcctatga tgcctttagc aaacgcacaa
    31741 agttcaaaag ttgttcatat gttggaagct caccggccaa ttgcactaga gacccatccg
    31801 aataactttg ttcagtggcg ttttactgct aaggagcatc cagaagcttt ttcgggtatc
    31861 cgatactatc actccacttt tgatgctata aacaaccaga caatgaagac atttttgcga
    31921 acgtcgatgg cacaggatgc ggtatttctt caagtatatg gccaaagtga atgcggacca
    31981 atgattctta aggctcatac actagagagt ttgaaaacaa gtgatgcaag agatatgggc
    32041 gttggactgg aagacatgac aagcgctcgg attacagatt cagttgggaa tgtcctacct
    32101 gaaaacacag atggtcatat acaattactg tctaaaggac gggctttgac ttattacaag
    32161 gaagatgcac ggttccagga aaatgtttat ggcgattggt gggatagcgg agattacggt
    32221 tttatggata agcatggcca cctatttttg aaagatcggc aagtggattt gatcgaaacg
    32281 atcaatagta cattagctat tgaagacttt ttattagatt cgttagattt tttggcagaa
    32341 gtagtgattg taagggataa aaatcattct ccacaaccga ttattgcttt agctcctgat
    32401 aaagaaatgg attggaatcg atggtgggag caagtccatg aacttcctcg cttgaacgtc
    32461 cctatcatcc gtgattttga tgcaatcccg cgaacagcta caatgaaagt gcaacggctt
    32521 caaatcgaga aagaattaaa atcacaaaag tcctaactat ttaaggaaag aaagatccat
    32581 tttggtcttt cttttctttt tgttttttta aattggatga gataaaaata tgccaattgg
    32641 ctgtttttct tttttgaaaa tttcgttata ctcttattca tggaggaaaa tgttatgaaa
    32701 agaatcttga caattggcgg ttctgatcca tttgctggtg gaggaatcca aacagattta
    32761 aaaacttttg agaattttgg tctattcgga ctaagcgcct tgacttctat aggggcacta
    32821 gatattaatg agcgatttat gctggaatct atatctttgg aattgcttaa acgacagtta
    32881 gtttcgattg ataaaatgac ttcattagat ggtatcaaaa ttgggttatt gaattcaagt
    32941 gaagcaatca tggttgttcg agacttcata aaaacaaaac aaaatatccc aatcatttta
    33001 gaccctgttt tggcattcaa agaaactgaa gctgtgatca atcaattgta tatttcgcag
    33061 ctcatcgaag aactttttcc acttgttacg atgattacgc ctaatttggc agaagctgct
    33121 ttactttctg gtcaagctct tccctcttcg ttaacagaaa tgatccatct cgctaaagaa
    33181 ctcactgctt caggaacaaa aaacattgtg atcaaaggcg gcacaaggat ggctggagat
    33241 gaagctgtgg atcttctcta ttcaattgat cattatgagg tattttctca taaaaaaatc
    33301 gacactacaa cagtaaacgg ggccggttgt gccttttctt ctgcaataac agcgaatctt
    33361 gtgttagggg cagattttac caaagcagta gcagaaagta aaaaatttgt tgcccattgt
    33421 atttctaatg gcgtgctgat gaacgatcat accggaagtg tctggtctgg cggtattcaa
    33481 aaaggaggaa tgagaaatga aaactaagca attaacgatc tatgctatgc tgattgcgtt
    33541 gacagttgcc ctttcattaa caattttgat ccctgtaccg gctacgaatg gatttgtgac
    33601 attatgcgag gccgggatct ataccgctgc ttttctttat ggtccaacgg gtggattagt
    33661 tgttggtgct tccagtggtc tattgatcga tttgatttca ggatatccgc agtgggcagt
    33721 tttttcatta gtgatccacg ggttacaagg atatgttgct ggttctttca gcaaatcttc
    33781 tactcgtatg tggggattag ggcttttatt aggaagctta gttatgatca tcggctatgg
    33841 tatcgccggt tggattttat atgattggcc ggctggagtg gcttctatac caggaaacat
    33901 cttccaaaat ctgattggaa tgggacttgc ctttcctata actgcaagtc ttcaacgtat
    33961 tcaagcacgg aagcaatatt aattgaaaaa gaaggtttta gaaaatgaag ttgtcagaaa
    34021 aagaattaaa agaccaattg actgaaatag ttaatgacat attggctgag gcacacttga
    34081 aaaaaggtga tctttttgtt ttaggatgca gcacaagcga agtcgttgga ggacatattg
    34141 gaaaaaattc cagtgcggaa gttggtcaat ggatcatccg tacgttaaaa gagttgttgg
    34201 atcctaaaga aattgcgcta gctgtccaag gttgtgaaca tttgaaccga gcattagtag
    34261 tagaacgaac agtggcagaa gcaaaaaatt tcgaaattgt ctctgttgtt cctgcactac
    34321 atgctggagg tgcttgttct gttgctgctt ttgaccagtt caatgatcct gtcgaagtgg
    34381 aacacgtagt tggacaagcc ggtatcgata tcggtgacac atcaatcggt atgcatatca
    34441 agcatgtcca agtacctgtc agacctcgtt tgaaaaccct tggacaagct cacgtgacag
    34501 cccttcgctc tcgtcctaaa tatatcggtg gtcctagagc gaattattga aatgagtaat
    34561 tgacagagca atctgaaaaa cttcctcgca aagtccaact aatgaacttt aacgaggaag
    34621 ttttacttgt ttgttttata taaccgagct tcataaggtg ctagtatgcc tttagcttca
    34681 aattctccgc tagaggaatt tgacaagagt aatgtccagg actgatctgc ttcttttgga
    34741 agttcatagt cagcaagtga ttctgttaag tttaccataa taagatattg ttcatcttct
    34801 aaatatcttt catagacata aagttgtgga tgttcatgaa gatattcttt ataagaaccg
    34861 tatatcagac ctttattggt ttgtctaatg cggatcatgt tcttatagaa ttgcaggacc
    34921 gactctggtt catattcttg ttgttctaca ttgatcgttt tggtatttgg attagtgatc
    34981 agccaaggtt catgtgtaga aaaaccagca tattgttccg aagtccattg catgggtgtc
    35041 ctgctgttgt ctctcgttgt ttcacgaatg atatccaatg cttcttctct tgtcttacct
    35101 tccgcaagca gccttttata aagtcttttc gaatctactg catcaacttg atcgatcgat
    35161 tcaaagggca tattcgtcat tccgatttcc tgtccttgat aaataaatgg cgtaccttgc
    35221 aaaaagaaat acatcatagc gattgctttt gcagacatag cccaaaaatc gggacgagta
    35281 tctccaaaca ctgaaacggc acgaggaaca tcatggtttt ccatatataa agcgttccat
    35341 ccgataccat ccaatgaagt ttgccaagcg cttaatgcat gtttcaattt aaggacatct
    35401 aattgccctt ctttttcttg ctgccagata tggatatgat caaattcgaa tatcatatcg
    35461 aaataaccgt ctttccctac ccattgtggt ccttcttctg cagtgacacc acttgcttct
    35521 ccgacagtca tgatatcgta ctcttcaaaa acatgtttca aatcggtcaa atgttcttcg
    35581 attcctgaga cattttgaaa gggtgagaaa gggttctctg tggcttttac tgaatattca
    35641 tcttttttta catgagaaat cgcatctaaa cgaaatccat ctatcccttt atccaaccac
    35701 catcggatca tattgaatag ttcttctttc aatttctcgc tttcccaatt taagtctggt
    35761 tgttcttttg caaaaacatg cagataatac tgtccagttt catgagaata ctcccaagca
    35821 gaaccgccga aaatcgatgt ccaattattc ggttctttgc catctacccc gtcgacccaa
    35881 atgtaataat cgcgatacgg gctttcttta gattttttcg attcttcaaa ccattgatgc
    35941 tgatccgaac tatggttgat gacaagatcc ataatcactt tgatccctaa ttcatgtgct
    36001 tcttttagca ataaatcaaa atcttccatc gttccgaatc tctcttctat atcttgatag
    36061 tcacttacgt cataaccatt atcgacaaat ggagattgat agatcggatt gatccagata
    36121 aagccgatac caagatcttt caaataatta agtttttcac ggatcccatt gatgtctcct
    36181 tgaccatcgc cattgctatc tttgaaacta gcaggataga tttgatagcc tactgcttct
    36241 ttccaccatt tttgttcttg tatcatatcg tttcactcct ttaacggtcc ctcttgatta
    36301 taaccgattc tattttagaa gtatccttgt taccggtaac tagaaacgaa aagctcaacg
    36361 aatctgctaa gtgcgttcgt tgagctttat tttatcatag atagcgggca atcagttgaa
    36421 tcatttgctt gggaaattca ttcaagtcag taatatctac aaaacgactt tcaccgtaga
    36481 tcgacttgat ttcttctttg tcttggccaa tagctgcgga gacaaatagg attccttgac
    36541 gttcatattc tttcaaaaca gactggatat cttcttttgc ttttttgcct gtataatccg
    36601 gcaatgcttt tggctgaccg tcactaatat taagcaacag tttggttgtt gcggattgtc
    36661 tgtttaattt ttctgctgct aaacgaagtg cagcaccatc acgattgttt tgccttggtt
    36721 tcatagtgac taatttttca tttaaccatt gaaatccgtc ttcaaattct ttataagaaa
    36781 acaaagaagt cttttctctt gacgaacggt ctgctgtatc tccgtaaatc aacaatggga
    36841 tatcgacttt cttagaaaat tcggcaatgg caatcgctgc tttttttgcc gcttcgatcc
    36901 gatcatcccg tatcattgac ccagattcat cgatccgtac agctaccgct aaagaaggtt
    36961 gctcatgtgg cgggttcttt ttgtcaaaat ttctcaaatc accatatgct actttgcttg
    37021 cattgaaccg ctgaccttcg tattttcctt tttggtacgt ctctgtttgt tcattatcca
    37081 gtaactctaa aatttgccgg ctaagtgatt ctactattgg cattacttgc atgtgtagct
    37141 cacgtgcttc cggcgttttt ccttggaaag ctggtcggtg aacgatgaga ccttcttttt
    37201 catgaatcgt ttccttcatg atttgtcgcg cttctttatt cagttgcttc ttgatttcct
    37261 tatcatgtgc ttccatgtca gaagcagaca tttcttgaga atcatctcct gtttgttgtt
    37321 ctttttgatt ttttccttcg atatcgctac tgcgttcttt ttgcatttga ggaggaatct
    37381 cgtttttttc tattgtttct tcttgtttct cttctccctc atttggttct tcagcagaca
    37441 acgatttctt ttcatctaag cgcagaattt cttcagcatt ttcagtgatg tttcgttcat
    37501 ttgcttcttc ggcgctaatg gttctttctt ttgtatgctc agattgagga tgaccccatt
    37561 tttcttttct caatatgtcc tgaagttcca gtagcaatcc actgtcagac aaagcttgtt
    37621 caagaattgc caattcatct gaatcagtgg ataatttata caaaactttt ggataaatcg
    37681 actggagtaa atcttgtcct tgcttatagg tatttgccca ataaaaataa gaacgcattc
    37741 ctgctacacc tttgatggcg ttagcttttg cagtttcatc caacaaacgg ataatctctg
    37801 ccatctttaa gagaagttct tgtttttgga tcatagtttt cgccatcgca cgttctgcca
    37861 tgacttctaa ggcaggaaga tccatttttt ccgcatgttg catccgatct cttaaagatt
    37921 catttaatgg gcgatttcct tggtaatttc gatttgtagt gatcacaatg acgcaatctg
    37981 gatggcgacg aatgattcct gtcggtaaat tcaacatccc atttggttcg agcgctgagt
    38041 taagtgccac aagaacagag gcatctcgga tgacagtcgg ctcttgaatc tctaataaat
    38101 agcctttttc aatcgctcga acgatttcag aagggtaata gctgtaatgg attgcatctg
    38161 gataatcagc ttcgatcctt tgaactagct ctgctaattt ttctttagca gaaaaaggat
    38221 caatgccgta atggttctgg atcagtgtca gtacagcttc aagtgtatct gtttggtaaa
    38281 tagcttctag taattcttgg tcttgttcct tattagaatc tactacaggt aataaggctc
    38341 cgaaaacatc tgatttgtcc atatctgcaa aacaggtcac ttttgtatag ggaagttgca
    38401 gatcagcaga tagtgctttg gctaattgtg tcttccccga cccggcatct ccttccaaaa
    38461 gtacgttact gattttcatt tcaggatctt gccaattccc tttgatttcg gaggcgatcc
    38521 gccattcttc tgcacttgtc cgatggcttt tcggtttctt ccagatcatt tgcttttcta
    38581 gccgtgaaaa ggttcggttt tctgttagtg agaaattctc catgcttctc ttcccctttt
    38641 gatcatctct gtctattaat atcctagctc atctaaaaga cgttttaaaa tacggaatcg
    38701 ttccccaatc agttctccgt ctttttgtag tgtatcgtaa tacaaaatga tgtcttcatc
    38761 gatctttgga tttttggtat ctacttctac cgtcattcgg ttcccttgta atccaatgat
    38821 gtctacgact ggattttctg gatcataaaa tttttcataa cgataagcat cttggaagta
    38881 taaacttcct tgcgcagcta agatcgctaa atctaaaata cgtgtgattg gtaattcttc
    38941 cgactgtcgt gaccattttt ctccagtatg tcgccatacc ttgcctgaga tatcgacttt
    39001 tccacgatca ttccactgag caaggcccag tgaaagccct tttgcatcca tatttaatgc
    39061 atttcttcca tcaactttat catagtcttc tacaacgatt actggtttat gttttaaatt
    39121 tgttggtatt tccattattt taactcctca tccttttatt tttagtaaat aagtaaatta
    39181 gtagtttact catttactaa actatatgcg acattataga ggattgaagt gaggattgca
    39241 aagaaaaaga gcttcattga tagaaataaa aacaccatct cgttctttta cacgctcatt
    39301 agtaacatgt agaaagtcca agatgatgtt ggaatatttt ggctctatac tttttgatgt
    39361 tggtgaactt caccaacatc ttttttgta
//
LOCUS       DARDHU010000014        39243 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000014 DARDHU010000000
VERSION     DARDHU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.014"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..561
                     /locus_tag="SDD03_000794"
     CDS             <1..561
                     /locus_tag="SDD03_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG4303263.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            761..1075
                     /locus_tag="SDD03_000795"
     CDS             761..1075
                     /locus_tag="SDD03_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4303264.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1096..1473
                     /locus_tag="SDD03_000796"
     CDS             1096..1473
                     /locus_tag="SDD03_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4303265.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1483..2256
                     /locus_tag="SDD03_000797"
     CDS             1483..2256
                     /locus_tag="SDD03_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303266.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2278..3681
                     /locus_tag="SDD03_000798"
     CDS             2278..3681
                     /locus_tag="SDD03_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4303267.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3863..5047
                     /locus_tag="SDD03_000799"
     CDS             3863..5047
                     /locus_tag="SDD03_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4303268.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5044..5334
                     /locus_tag="SDD03_000800"
     CDS             5044..5334
                     /locus_tag="SDD03_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303269.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5331..5552
                     /locus_tag="SDD03_000801"
     CDS             5331..5552
                     /locus_tag="SDD03_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303270.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5594..6373
                     /locus_tag="SDD03_000802"
     CDS             5594..6373
                     /locus_tag="SDD03_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG4303271.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6440..6934
                     /locus_tag="SDD03_000803"
     CDS             6440..6934
                     /locus_tag="SDD03_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4303272.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6990..7628
                     /locus_tag="SDD03_000804"
     CDS             6990..7628
                     /locus_tag="SDD03_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303273.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7700..8095
                     /locus_tag="SDD03_000805"
     CDS             7700..8095
                     /locus_tag="SDD03_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4303274.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8079..10532
                     /locus_tag="SDD03_000806"
     CDS             8079..10532
                     /locus_tag="SDD03_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4303275.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10529..12556
                     /locus_tag="SDD03_000807"
     CDS             10529..12556
                     /locus_tag="SDD03_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303276.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12553..13575
                     /locus_tag="SDD03_000808"
     CDS             12553..13575
                     /locus_tag="SDD03_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG4303277.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13592..14521
                     /locus_tag="SDD03_000809"
     CDS             13592..14521
                     /locus_tag="SDD03_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4303278.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14766..14882
                     /locus_tag="SDD03_000810"
     CDS             14766..14882
                     /locus_tag="SDD03_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG4303279.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14898..15701
                     /locus_tag="SDD03_000811"
     CDS             14898..15701
                     /locus_tag="SDD03_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG4303280.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15936..16172
                     /locus_tag="SDD03_000812"
     CDS             15936..16172
                     /locus_tag="SDD03_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303281.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16179..18131
                     /locus_tag="SDD03_000813"
     CDS             16179..18131
                     /locus_tag="SDD03_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG4303282.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18203..18700)
                     /gene="dfrG"
                     /locus_tag="SDD03_000814"
     CDS             complement(18203..18700)
                     /gene="dfrG"
                     /locus_tag="SDD03_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG4303283.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19062..20102
                     /locus_tag="SDD03_000815"
     CDS             19062..20102
                     /locus_tag="SDD03_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4303284.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20200..20388
                     /locus_tag="SDD03_000816"
     CDS             20200..20388
                     /locus_tag="SDD03_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG4303285.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20446..20799)
                     /locus_tag="SDD03_000817"
     CDS             complement(20446..20799)
                     /locus_tag="SDD03_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303286.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21330..21752
                     /locus_tag="SDD03_000818"
     CDS             21330..21752
                     /locus_tag="SDD03_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4303287.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21749..21979
                     /locus_tag="SDD03_000819"
     CDS             21749..21979
                     /locus_tag="SDD03_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303288.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22477..22677
                     /locus_tag="SDD03_000820"
     CDS             22477..22677
                     /locus_tag="SDD03_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG4303289.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22704..23897
                     /locus_tag="SDD03_000821"
     CDS             22704..23897
                     /locus_tag="SDD03_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4303290.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23965..25530)
                     /gene="guaA"
                     /locus_tag="SDD03_000822"
     CDS             complement(23965..25530)
                     /gene="guaA"
                     /locus_tag="SDD03_000822"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG4303291.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25589..26512)
                     /locus_tag="SDD03_000823"
     CDS             complement(25589..26512)
                     /locus_tag="SDD03_000823"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG4303292.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26708..27313
                     /locus_tag="SDD03_000824"
     CDS             26708..27313
                     /locus_tag="SDD03_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4303293.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27621..28637
                     /locus_tag="SDD03_000825"
     CDS             27621..28637
                     /locus_tag="SDD03_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4303294.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28778..30079
                     /locus_tag="SDD03_000826"
     CDS             28778..30079
                     /locus_tag="SDD03_000826"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG4303295.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30125..30787
                     /gene="deoC"
                     /locus_tag="SDD03_000827"
     CDS             30125..30787
                     /gene="deoC"
                     /locus_tag="SDD03_000827"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG4303296.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30808..31206
                     /locus_tag="SDD03_000828"
     CDS             30808..31206
                     /locus_tag="SDD03_000828"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG4303297.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31272..32354
                     /locus_tag="SDD03_000829"
     CDS             31272..32354
                     /locus_tag="SDD03_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4303298.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32566..34128
                     /locus_tag="SDD03_000830"
     CDS             32566..34128
                     /locus_tag="SDD03_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303299.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34121..35254
                     /locus_tag="SDD03_000831"
     CDS             34121..35254
                     /locus_tag="SDD03_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303300.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35254..36210
                     /locus_tag="SDD03_000832"
     CDS             35254..36210
                     /locus_tag="SDD03_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303301.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36309..37475
                     /gene="deoB"
                     /locus_tag="SDD03_000833"
     CDS             36309..37475
                     /gene="deoB"
                     /locus_tag="SDD03_000833"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG4303302.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37499..38317
                     /locus_tag="SDD03_000834"
     CDS             37499..38317
                     /locus_tag="SDD03_000834"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG4303303.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38334..39041
                     /gene="deoD"
                     /locus_tag="SDD03_000835"
     CDS             38334..39041
                     /gene="deoD"
                     /locus_tag="SDD03_000835"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG4303304.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        13000 a         6775 c         8023 g        11445 t
ORIGIN      
        1 ttaaaaaatg aatttggaat agaagttaaa gattatttta ttgacttaga tttattacag
       61 ctaataaaca gtgtgaaaag aatgtatagt ggagacgaca acggtgttga tttatacaaa
      121 tttgttacca aacatatatt tgttaatctt aatattgacg agaaaaatta cccgtctatt
      181 tttaagcact atctattttt tattaacgaa gtagaagatc ggataaaaaa taattcatta
      241 tctagtagtg tatctagttt taagaagatg tttaaatctc gagatggtat cacacttact
      301 acagcacata aagttaaagg aaatgagtat gatactgtta tcgctattaa tcttctaaac
      361 gggaaaattc ctcattggaa tgaaattttt aatgtttctg ataaaggaat tagttcagcg
      421 agaaaactac tatacgtaat ttgttcaaga gcaaaaaata atttatttct tttctcagag
      481 gtgggttatt ttacgagtaa aggagacttg ctttatccca ctcaattatt agataaaata
      541 gaatttgatt ataatattta aaatacctag gtagtcgttg ttttaaatat agtaggaatc
      601 ggacgctcag tacggagcgt ctattttttt acccaatttt gaaaggaagt gaacttatga
      661 aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt taaaaccatc acacaaataa
      721 gattcattca aacataaaaa cagaaaggat aggtaaaaat atggaactta aatatgtcat
      781 tcccaatatg gaaaaaacat ttggacaatt agaatatgct ggtgaaggga acattgaaca
      841 acgtcgaata aatggcagac caaccattct ctctcgtagt tacaatttat actcaacgat
      901 tcaacgagca gatgatattg ttgtcattct tccctccgaa gctggcgaga aacattttga
      961 atccgacgaa aaagtcaaac tcatcaaccc acatattacc gcagaaggtt ataaaattgg
     1021 caatcgagga tttaccaatt acattatgca cgcagacgat atggtgaaag cataagaaag
     1081 gaaaggtgaa aaaatatgag attagcagaa ggtattgtga ttgaaaaaga ccagacattt
     1141 ggcacattga aattttccgc cttgcgtcgt gaggttcgta tccagaatga agatggtacg
     1201 gtttccgaag aaattaaaga acgtacctat gacttaaaat caaaaggaca aggacgcatg
     1261 atacaagtca gtatccccgc ttctgttcct ttgaaagaat ttgactacaa cgcagaagtg
     1321 gaattaatca atcctgtggc agataccgta gcgacggcta cttttcgagg ggcagacgtt
     1381 gattggtacg tgaaagcaga cgatcttgta ttaaaaggac agacaaaacc tcatcagaaa
     1441 tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc ttatgacaaa cacaccattg
     1501 attttgaaag aaattccgaa ggatgaagcc atttctttta ttcggcaata ccattattca
     1561 aagatactcc ctagattgtg taaatacttt ctagggattt tttcagaaga aaaattatta
     1621 ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa ccatacgcaa actctttccc
     1681 gacagttccc ttcaaaccac agattattta gaaattggaa agatgtgttt tcttccagaa
     1741 atgaatcaaa ccaattattt tggcagtcaa gctctttcag ctctaattaa gtggttgaaa
     1801 gaacatacgg actgtcattt tttatataca ctggcagatg gaattgaggg aaaatgtggc
     1861 tatgtctatc aagcgtccaa cttcttttac tgcgggtatt tcaagacaag tgtctatcgt
     1921 gataagcagt catgggaaaa gatacaccct cgaagtgctc gccttttatt agaggaaaat
     1981 gctcgctttg aacaggtaga gaaaaagcat tggctttccc aggcattttg cgaatacaaa
     2041 gggattgaga aaatcaatgg acgcatgttt cgctatctct atccgttgac aaaagaagcg
     2101 aaaaaactgt taggacacac tctctatcga cgtcattatt atccgaaaga aaaaaattta
     2161 cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca tttctcaacc aacttttgat
     2221 aaacaggctc gtatctacaa tacacaatta ttttgatagg tggaaaggag gatttttatg
     2281 ccattgcttc atgacagagg agagcgaatc aaagccaacg ataagcactt ggtctatcat
     2341 tttgtactaa tttcattgct ggtagttttt ctagtcgtcc tactcccttt tcattggaga
     2401 gcgttacaat cggtgaactg gcaagcagtt cagttagata catttttcta tcaacttcat
     2461 acgccatact tttggagcag tattctcact gctctttttg tatgcggact tatcagctgg
     2521 ctttattatc actatcgtat agattctata aaacagttag agcaccgtca aaagttagct
     2581 cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa ctcaatcaga gggattcttt
     2641 aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc attttcctaa aatatattat
     2701 cgaatgaaag atggactaat ccatattcat gtagaaatca ccttagggaa atatcaagat
     2761 cagcttttgc acctagaaaa caaactagaa acaggattgt attgtgaatt agtggaaaaa
     2821 acgttgaaag attcttatgt agagtatgtc ctactttatg atactattgg caatcgaatt
     2881 ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat tgatggaatc ggtctattgg
     2941 gagttcgatt ctctccctca catgttaatt gctgggggca ctggtagtgg aaaatcctat
     3001 tttattctca cattgattga atctctcctg catacaaatg caaagcttta tattcttgac
     3061 cctaaaaatg ctgatttggc agacttaggg gcggtcatga ataatgtcta ttaccgtaaa
     3121 gaagatatgt tggcatgtat caatcaattt tacgaagata tgattgatcg cagtgaaacc
     3181 atgaaacaac accctaacta taaaacagga gaaaattatg cttatcttgg tttgtctgcc
     3241 aattttctta tttttgatga atatgtggca atgatggata tgctaggtcg tgaaagtacc
     3301 accgttattc ataaactcaa acaaattgta atgttaggac gacaagcagg cttcttttta
     3361 attcttgcct gtcaacgtcc agacgcaaaa tattttagtg atgggattcg ggacaacttt
     3421 aacttccgtg tggctttggg tcgtatgtct gaaatgggct ttggtatgat gttcggaagc
     3481 gatacacaaa aacaattttt cttaaaaccc atcaagggaa gaggttacgt ggatacagga
     3541 aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa aaaggtacga tttcttagga
     3601 gaaataggaa aagtcattca aaaaaagcaa tctgagcctg taccgcacga agtgaaaggt
     3661 acaggttcag acttgctttg attggtgtgg cgttagccac gccaatcctt tagccccctc
     3721 tatctaacag gggggcacaa atacaactga aaacaggcaa ttctcatcaa ccaatgttga
     3781 tggtacaaag gattgcggaa aatatgaaag gtgtaaactc aagggtcata gtttacatct
     3841 tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt tggattaagg aattgaaaga
     3901 aaagcgactg gcttatggcg tttctcaaaa taaactggcg gttgcttctc atattaccag
     3961 accttaccta tcggatattg aaacaggcaa ggcagtccct actcaacagg tgaaagaaga
     4021 tttactgaac gctttggaac gcttcaatcc cgataaccca ttggagatgt tattcgacta
     4081 tgtacggatt cgattcccta ccaatgatgt ggctcaaatt atcggtgagg ttttacgttt
     4141 gaatatggat tatatgctac acgaagactt tggctattat tcttatcccg aacactatcg
     4201 tttcggtgat attgttgtcc tagtctctca tgatgtaagt aaaggtgttt tattagaact
     4261 gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg gctcaacatc ggagctggta
     4321 tgatttcttt caagattgca tggaacataa aggtattttc aaacgcttgg atttagctat
     4381 caacgataaa acagggattt tgaatattcc agatttggca aagaaatgta aacaggaaga
     4441 atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt ggtgaattgg ttcaccgtga
     4501 tgaaaaacca tacatgggaa acacacttta tattggctca ttaaaaagtg aagtctattt
     4561 ctgtatatat gagaaagatt atgagcaata cgtgaaaaat gatgttccct tagaagacgc
     4621 agaagtaaag aatcggtttg aaattcggtt aaaaaatgac cgagccaccc aagccatgaa
     4681 agatttattg gcacatcaac aggcggaaaa aacagccttt gaaattatca atcgctatat
     4741 ccgctttgca gataaagacg acactaaacg tcggagtgat tggaaaacta atgaacgttg
     4801 ggaatggttt atcggtaaaa atcgtggagc attacgccta accacacagc cagagcctta
     4861 ttcttttgaa cgaacattga actggttaca tcatcaagtc gcccctacat taaaaatcgc
     4921 aagcatttta gatgttctca acggtacaac cattatctcc accatgattc aggaagccaa
     4981 actgacagaa aaacatgaga aattaattga acaacaacat ctagccatgg aggatttgat
     5041 tacatgagcc ttttaacaaa agaattaaag aagttagggt ttcagtgtgg cattgagttt
     5101 caagcgtata tacaaaatac agggaaatat acgtctctca ttattgaggg aaaacggcaa
     5161 gctggtgata ccatctatac ctatgatttt tacaaggtga gattttataa taattatacc
     5221 aatcgagtaa ccgtctatgg agagcattta accccttttc aattattaag gcgtgtaaaa
     5281 agctatatct attatcgaga aaaatatctt aaggaaagga ggacaatcac atgaattttg
     5341 ggcagaattt gtacaactgg ttcttggata atgcacagtc attggtattg ttagcgattg
     5401 ttgtcattgg actgttttta ggttttaaac gagaattttc aaaactcatt ggcttcttga
     5461 ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc tggcgtgaaa gatgtgttat
     5521 taaacttatt taaccgaatc attggtgctt aacagatatt tttcatgata aaataggaag
     5581 tatatatttt gttatgaaag gatttatgaa tatgtttaca aatgatcaat ttaaatctct
     5641 tttaattaaa acattagaaa gaaagagctc ttcaaattat tatgaaggag gtaacgagat
     5701 tgtttctaaa atgaaaatat cagaggtaac tttagatgag actgacgatt ttacttatta
     5761 taaaggatat aaaaagggct ggaatacgct ttgcacatat cttaaaataa gagttccttt
     5821 tgatgattta gacttttttg aaagtcataa agatttgatt acccagacag caagctccat
     5881 atatgataaa caaggtgata atgtattagt ggatactatt ttagtaccac ttccagagaa
     5941 ttacgaagta attaacttta gtcagttgaa aattagtgat gtggtttcac aagctattga
     6001 agacgctgag tcttttatga gtaatggtga atatcaacgt gcctttgaca gagttcacac
     6061 tgcttttcat ggatatttaa ttgaaatact aaaaaaatat gaaattacag ttcctagaga
     6121 cgaaaatcta tctaaattat atagtaggat acaacaatta attgaaaaag aaattcaacc
     6181 tactgaactt gctgatatag tgaagacaac gataaggtca tcaaatggaa tgattagctc
     6241 tcttaatgaa gcaagaaata ggcattcact tgcacaccct aatacaaata ttattggaaa
     6301 gagagaagca aaactaataa taggtatttc aagtacagtt acagattata ttagcggata
     6361 ccttgataag tagcgtcatt aactgattgc tacttttttt atgactattt ttataaaaag
     6421 gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat ttaggacggt ataatgaagg
     6481 tgaattagtc ggagcatggt ttactccacc aattgatgag gaagaaatgg cagaaagaat
     6541 cggtttaaat gaggactacg aagaatatgc aatacatgat tttgagcttc cttttgatgt
     6601 ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt gaagccattc aagaaatcga
     6661 aggtacacct atttataatg aactcaaaga aattcaaggc atgtggttta gtagcttgga
     6721 agaattactg gaaaacaaag aggatattca ttgttatagt gattgtgatt cgatggaaga
     6781 tgtcgcccgt tattacgtgg aagaaacagg acagcttggt gaagtccctt ccaatttaca
     6841 aaattatatt gactaccaat ctttgggacg tgatatggaa atagagggta attacctagt
     6901 tacctctcat ggggtatttg aatattgtca gtaagtttaa caatccgtgt tataattaaa
     6961 ttaataaata gcacggagag gtataaatta tggtaaatgt atttgaaaat cttaaactat
     7021 taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt cgttacaaac
     7081 agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt ccagaatatg
     7141 ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta gctgtctatg
     7201 ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt ggtatcgaat
     7261 acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct cgatttattc
     7321 cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt ttctcattaa
     7381 gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga agaaatcctt
     7441 taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa acgagattgt
     7501 atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc agatattcaa
     7561 tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca aaaaataaaa
     7621 ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt ttctattttt
     7681 acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt ggtcagtaga
     7741 aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct ttacgcaaat
     7801 gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac caccactttc
     7861 tctgattgac ggagcactac tcaaatactt agggattccc attgttctca ctgggttcat
     7921 gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta tcattaccta
     7981 tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa aagaaacaat
     8041 gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg attaaatata
     8101 tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat gaattaattc
     8161 cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat aatttctgtc
     8221 aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca accgaatcaa
     8281 gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta aaagagatag
     8341 cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt gacaatcaag
     8401 tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa gtatccgtaa
     8461 aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt gtgaatcaca
     8521 aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt tctaagatgg
     8581 aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg gagaaagatg
     8641 attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt gatgactacg
     8701 actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa tacgatattc
     8761 tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt gaacaggaag
     8821 ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa ttggatttcc
     8881 ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt gataccagta
     8941 tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat aaaaagaaag
     9001 aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt actaatgtgg
     9061 tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc aaagaatcta
     9121 tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag ctgaaacaac
     9181 gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta cgaccttttg
     9241 gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat atcaatgact
     9301 atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct acacaaatga
     9361 ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga aatatttatc
     9421 tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca ttagcgtccg
     9481 cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg gtctattatt
     9541 cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt ggtgaatgga
     9601 aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg agtgagaacg
     9661 acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat tcagaaagcc
     9721 ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc gagaagtttc
     9781 ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg ttgttattgg
     9841 tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct gaccatatcg
     9901 aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt gtcacccaat
     9961 ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc ttacccgatt
    10021 ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca atgctgattg
    10081 tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt aaaattgttg
    10141 accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta tctatgcgat
    10201 tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa aacgcggacg
    10261 atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt cgttccactg
    10321 acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa gatgaaaaca
    10381 atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac atctatggtc
    10441 gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc tttgatacca
    10501 gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat ttcttcacta
    10561 tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca cgctgggcaa
    10621 tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg aatactccaa
    10681 gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg gctggcttcc
    10741 atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg ccattactaa
    10801 ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg tgaaagaagc
    10861 ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca tgcagacgat
    10921 tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt tcctccttct
    10981 ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc gagaaacgac
    11041 gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg cttcctttat
    11101 tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg tcagtaccgc
    11161 cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag ggaaagacag
    11221 tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat ggctattgct
    11281 tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa gtttattgtc
    11341 taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg aagaaattga
    11401 agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag gaacagtctt
    11461 ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa caggaatcat
    11521 gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca gtttcctatt
    11581 aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt tatttaatgt
    11641 gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta tttccactat
    11701 gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac aaattgtgac
    11761 ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt tacaaagcaa
    11821 cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga tgaatcgaca
    11881 aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag ggtcaacgaa
    11941 gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata ctcgtcaaaa
    12001 tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc aacaaggcgg
    12061 tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa atcgtctagt
    12121 caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg tcaaatatgc
    12181 ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac aatctatggc
    12241 agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa aacgaaagaa
    12301 cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa aagaaaaccc
    12361 atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa ccgtcacgcc
    12421 tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa aagagcaaaa
    12481 acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa cggataagcg
    12541 aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt ctctctcttc
    12601 ttttttctct tattattcct agtcattttc tttgcagatg atacggacag tggcgaaaac
    12661 aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt gttggcacac
    12721 cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt ttcttatatc
    12781 ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca aagttccgaa
    12841 tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca aggggttaaa
    12901 tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga ttctgttatc
    12961 caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg gaaaaaatat
    13021 acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa agcagactat
    13081 cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg aaatcagttt
    13141 tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt tgatgatgaa
    13201 accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta tgtctttgga
    13261 ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt gtatgacaaa
    13321 gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca agaaatctct
    13381 atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa tgcaggcaca
    13441 tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc aggcgaccca
    13501 atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg ggctagacgt
    13561 gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc gaaaagaaaa
    13621 gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc cgatgattaa
    13681 agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg gtagtgtcgg
    13741 atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg aaacagaaat
    13801 cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat cctttgcgaa
    13861 agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg aaaaattgac
    13921 ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta aggatattcc
    13981 tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga atgagaacac
    14041 ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca aggaaactat
    14101 atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg ttattatcaa
    14161 aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac agctagaaag
    14221 tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag aaacgttctt
    14281 ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta atcatgtgtt
    14341 acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct ttaccagaaa
    14401 agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa caaaggcaac
    14461 ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga ttgtaaaata
    14521 acaaatattg gtacatgatt acagatactt tgtaatcatg tactcttttt gataaaaaat
    14581 tggagattcc tttacaaata tgctcttacg tgctattatt taagtgacta tttaaaagga
    14641 gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag cgggaacaaa
    14701 taattagatg tcctttttta ggagggctta gttttttgta cccagtttaa gaataccttt
    14761 atcatgtgat tctaaagtat ccagagaata tctgtatgct ttgtatacct atggttatgc
    14821 ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt ttgggttttt
    14881 gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat gttgatgcag
    14941 gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca gaattaggaa
    15001 gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag agaggaatta
    15061 caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac atcatagaca
    15121 cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt ttagatgggg
    15181 caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata ttatttcatg
    15241 cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac caaaatggaa
    15301 ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca
    15361 aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat
    15421 gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat
    15481 tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt tctctgttcc
    15541 ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata gaagttatta
    15601 ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga aatgttttca
    15661 aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag ttttggagta
    15721 caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt
    15781 ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta
    15841 tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt
    15901 tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata
    15961 agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat
    16021 acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt
    16081 tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg
    16141 aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag
    16201 ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag
    16261 atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa
    16321 tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt
    16381 actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa
    16441 aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta
    16501 tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt
    16561 tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag
    16621 atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat
    16681 tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc
    16741 aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt
    16801 atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt
    16861 cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg
    16921 agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa
    16981 caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg
    17041 tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa
    17101 cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct
    17161 atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc
    17221 atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac
    17281 catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta
    17341 ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact
    17401 attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact
    17461 tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac
    17521 atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg
    17581 gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc
    17641 tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag
    17701 aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc
    17761 cattagccac taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc
    17821 tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct
    17881 ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg
    17941 gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta
    18001 caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt
    18061 tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt
    18121 atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt
    18181 tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat
    18241 agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt
    18301 cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga
    18361 tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa
    18421 aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac
    18481 aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg
    18541 ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat
    18601 ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca
    18661 ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa
    18721 ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt
    18781 tagttgaata agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat
    18841 tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat
    18901 gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa
    18961 aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat ttacgagatt
    19021 cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact tcaataaatg
    19081 gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg caaaatgagt
    19141 ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg
    19201 aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa
    19261 tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg
    19321 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
    19381 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
    19441 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
    19501 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
    19561 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
    19621 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
    19681 gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt cggatgctta
    19741 ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta gagccatatc
    19801 ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac gatgctccca
    19861 aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt attattggag
    19921 aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt acaaatgggc
    19981 ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa cctgtttgcc
    20041 agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat aaaataactt
    20101 agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga aatattcttt
    20161 aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta caagtggata
    20221 ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac tatacttaaa
    20281 aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt tcaaaaaatg
    20341 aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt tgagaaatga
    20401 aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc cttcaatatt
    20461 tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt tgtctgtaaa
    20521 gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg
    20581 taagaaaaat tcatcaatag atacatggag taatgataca agatcataca gcacttgtgt
    20641 acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa tttcaatcat
    20701 tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa tcgctaaacc
    20761 aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc tctatacatt
    20821 ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag tttatgagtt
    20881 cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata gaaaggtcgc
    20941 tgattttatt tacctataca actttataaa atttagtcat actcctagaa agtagataaa
    21001 attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca tctagttctc
    21061 ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat gaccatcaat
    21121 gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga aaacaatctg
    21181 tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc atttatgcaa
    21241 caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca catgccactc
    21301 cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag
    21361 aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa gattacttca
    21421 aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat aactaccttg
    21481 tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt aaattatttg
    21541 gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat ctttcagaga
    21601 aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg gagattgcgg
    21661 agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga ctagccatga
    21721 ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg ttcccttccc
    21781 cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg ttaaacatta
    21841 tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg gaaattctca
    21901 tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct ctaagatttt
    21961 atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg tatccacgct
    22021 tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt aggttttgat
    22081 aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata cgtttcggta
    22141 agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg attcatgtta
    22201 gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg tgataatgat
    22261 actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt acggctacat
    22321 gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga ttgctaagat
    22381 attcttctct tgtcagtgta ttattataaa actatttgat ataattatag gtgaatattg
    22441 gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac aaaaaaggat
    22501 ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa agcaaaagaa
    22561 ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag agtccctcgt
    22621 gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc tgagtagttt
    22681 ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca aagaatggaa
    22741 cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt caaaaatatc
    22801 gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg ctattgatga
    22861 aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat atctttgaaa
    22921 ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat cgcttaccaa
    22981 ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt gagcctattg
    23041 atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat gtaagagctg
    23101 ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata accaccacaa
    23161 atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa ttttggacaa
    23221 aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat caacactttt
    23281 tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa gctactgcca
    23341 ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa acactctatt
    23401 acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc gttcgtcata
    23461 ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta caacaaaatc
    23521 taattcagac agactttgta atgagctata acggtattcc tacccaaaaa catacactat
    23581 catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga atccatgggt
    23641 tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt atcattaaag
    23701 atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat ctttatccta
    23761 acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa gaagcaaaac
    23821 aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga aacaaagaaa
    23881 tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac ccttggtata
    23941 agaggatttc tagctctcct acaactattc ccactccact gttgctggtg gtttagaagt
    24001 gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt
    24061 ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac
    24121 agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg
    24181 gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat
    24241 ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc
    24301 gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc
    24361 aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg
    24421 ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat
    24481 cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag
    24541 gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa
    24601 ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa
    24661 gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc ctagactttc
    24721 cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa
    24781 ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga
    24841 aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc
    24901 gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg
    24961 cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa
    25021 attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc
    25081 aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt
    25141 tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata
    25201 ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc
    25261 atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc
    25321 attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc
    25381 ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg
    25441 aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc
    25501 aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat
    25561 ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga
    25621 tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt
    25681 cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt
    25741 cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca
    25801 gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat
    25861 agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg
    25921 tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa
    25981 tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta
    26041 cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt
    26101 ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca
    26161 gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg
    26221 tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt
    26281 atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat
    26341 aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat
    26401 tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa
    26461 attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct
    26521 cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt
    26581 aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc
    26641 ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat
    26701 ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca
    26761 atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc
    26821 acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt
    26881 acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt
    26941 ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct
    27001 agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat
    27061 ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc
    27121 tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg
    27181 tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga
    27241 agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag
    27301 tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca
    27361 tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt
    27421 tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga
    27481 tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac
    27541 atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta
    27601 gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg
    27661 tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga
    27721 caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt
    27781 caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt
    27841 tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga
    27901 tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta caagagcttt
    27961 cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg
    28021 cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc
    28081 gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc
    28141 tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc
    28201 aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta
    28261 atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa
    28321 aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg
    28381 gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag
    28441 gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc
    28501 caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc
    28561 aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag
    28621 tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt
    28681 agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata
    28741 atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga
    28801 aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta
    28861 tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca
    28921 agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt
    28981 catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg
    29041 tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa
    29101 aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc
    29161 cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt
    29221 agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg
    29281 tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa
    29341 aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat
    29401 gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc
    29461 taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg
    29521 aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct
    29581 agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac
    29641 aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa
    29701 attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg
    29761 aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa
    29821 aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa
    29881 agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt
    29941 gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat
    30001 tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca
    30061 tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa
    30121 agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga
    30181 agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa
    30241 tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac
    30301 agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga
    30361 tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc
    30421 aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg
    30481 tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc
    30541 ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac
    30601 aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg
    30661 tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg
    30721 tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg
    30781 ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca
    30841 gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt
    30901 ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga
    30961 ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca
    31021 tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt
    31081 cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat
    31141 ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat
    31201 ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag
    31261 gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag
    31321 ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct
    31381 catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat
    31441 tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag
    31501 gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg
    31561 accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag
    31621 cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca
    31681 tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg
    31741 caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa
    31801 aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga
    31861 aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag
    31921 ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg
    31981 cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg
    32041 acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat
    32101 taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag
    32161 tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt
    32221 atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa
    32281 aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc
    32341 ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg
    32401 tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg
    32461 aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta
    32521 gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat
    32581 attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac
    32641 atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc
    32701 aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg
    32761 atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg
    32821 atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg
    32881 ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa
    32941 aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca
    33001 gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg
    33061 atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc
    33121 atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa
    33181 atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc
    33241 aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca
    33301 ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta
    33361 gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct
    33421 ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct
    33481 ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact
    33541 aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat
    33601 aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat
    33661 caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga
    33721 gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta
    33781 tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg
    33841 ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa
    33901 cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa
    33961 gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc
    34021 gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta
    34081 gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa
    34141 attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat
    34201 attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc
    34261 ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac
    34321 agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca
    34381 agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa
    34441 agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc
    34501 tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat
    34561 gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta
    34621 tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg
    34681 gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc
    34741 gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt
    34801 tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt
    34861 gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac
    34921 gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt
    34981 ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat
    35041 tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa
    35101 tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat
    35161 ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc
    35221 aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg
    35281 atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact
    35341 ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct
    35401 tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg
    35461 atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca
    35521 acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct
    35581 gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga taacatcacg
    35641 cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat tggaccaatt
    35701 ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt cctagcttgg
    35761 gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga aaatcctcaa
    35821 gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt actgatttct
    35881 ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg taacttttca
    35941 gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt tggtaagtgg
    36001 aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag cttgagtatc
    36061 attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca aattgctcca
    36121 tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg tccaaaagca
    36181 aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg gtgggttacg
    36241 ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag
    36301 gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga attggagaag
    36361 caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac attgcgaaag
    36421 aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc cgtcctcttg
    36481 aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa gaagtttctg
    36541 tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc aaaactccat
    36601 ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa gaattttctg
    36661 gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac gattacggtg
    36721 aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct gtccttcaga
    36781 tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc caattcgttc
    36841 gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg
    36901 gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg aatccattcg
    36961 gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct gttggaaaga
    37021 tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg
    37081 atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc agctttacca
    37141 atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg
    37201 cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa gaagacgact
    37261 tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc gatcatacgc
    37321 gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg gcattgcctc
    37381 aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg
    37441 aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga gaaagcaaat
    37501 gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata aaggagtaaa
    37561 agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag atgaaatcga
    37621 agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga cagtagttgg
    37681 gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag ccatgcaagg
    37741 gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag tacgtgtgat
    37801 ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg tcaacgaatc
    37861 atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg gtgaaaatcc
    37921 tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga gtcaagctta
    37981 tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt tggatcttaa
    38041 agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg agatccgaat
    38101 gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag tcatcgtagc
    38161 agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag cagcaggtat
    38221 gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca aacaatcttt
    38281 taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat aaaatgagtg
    38341 tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg ggagatcctt
    38401 tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac aatcaggtac
    38461 gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt caaggaacag
    38521 gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg tatggtgtaa
    38581 agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat gttcgtgatt
    38641 tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct
    38701 ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag atagctaaag
    38761 aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc tacaaagatg
    38821 atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa atggaagcag
    38881 cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg atgaccgtta
    38941 gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct acgttcaatg
    39001 aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat gaaaaagctg
    39061 ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt ttgacttggc
    39121 tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt tgtgaatctg
    39181 ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat
    39241 att
//
LOCUS       DARDHU010000015        37357 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000015 DARDHU010000000
VERSION     DARDHU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.015"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            75..470
                     /locus_tag="SDD03_000836"
                     /pseudo
     CDS             75..470
                     /locus_tag="SDD03_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="SDD03_000837"
     CDS             complement(465..683)
                     /locus_tag="SDD03_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303305.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="SDD03_000838"
     CDS             790..1104
                     /locus_tag="SDD03_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303306.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="SDD03_000839"
     CDS             1208..2386
                     /locus_tag="SDD03_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4303307.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="SDD03_000840"
     CDS             2789..3082
                     /locus_tag="SDD03_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG4303308.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3283)
                     /locus_tag="SDD03_000841"
     CDS             complement(3170..3283)
                     /locus_tag="SDD03_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HEG4303309.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            3925..6252
                     /locus_tag="SDD03_000842"
     CDS             3925..6252
                     /locus_tag="SDD03_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4303310.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            6378..6707
                     /locus_tag="SDD03_000843"
     CDS             6378..6707
                     /locus_tag="SDD03_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG4303311.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            6747..7751
                     /locus_tag="SDD03_000844"
     CDS             6747..7751
                     /locus_tag="SDD03_000844"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG4303312.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            7748..8194
                     /locus_tag="SDD03_000845"
     CDS             7748..8194
                     /locus_tag="SDD03_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG4303313.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            8344..9159
                     /locus_tag="SDD03_000846"
     CDS             8344..9159
                     /locus_tag="SDD03_000846"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4303314.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            9365..10537
                     /locus_tag="SDD03_000847"
     CDS             9365..10537
                     /locus_tag="SDD03_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4303315.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10699..11193)
                     /locus_tag="SDD03_000848"
     CDS             complement(10699..11193)
                     /locus_tag="SDD03_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303316.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            11776..12198
                     /locus_tag="SDD03_000849"
     CDS             11776..12198
                     /locus_tag="SDD03_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303317.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(12364..13557)
                     /locus_tag="SDD03_000850"
     CDS             complement(12364..13557)
                     /locus_tag="SDD03_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4303318.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            13781..14158
                     /locus_tag="SDD03_000851"
     CDS             13781..14158
                     /locus_tag="SDD03_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4303319.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            14146..14484
                     /locus_tag="SDD03_000852"
                     /pseudo
     CDS             14146..14484
                     /locus_tag="SDD03_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14474..14587
                     /locus_tag="SDD03_000853"
     CDS             14474..14587
                     /locus_tag="SDD03_000853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4303320.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            14618..15061
                     /locus_tag="SDD03_000854"
     CDS             14618..15061
                     /locus_tag="SDD03_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG4303321.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            15322..16137
                     /locus_tag="SDD03_000855"
     CDS             15322..16137
                     /locus_tag="SDD03_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4303322.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            16147..16809
                     /locus_tag="SDD03_000856"
     CDS             16147..16809
                     /locus_tag="SDD03_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4303323.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            17017..18681
                     /gene="alsS"
                     /locus_tag="SDD03_000857"
     CDS             17017..18681
                     /gene="alsS"
                     /locus_tag="SDD03_000857"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG4303324.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            18694..19404
                     /gene="budA"
                     /locus_tag="SDD03_000858"
     CDS             18694..19404
                     /gene="budA"
                     /locus_tag="SDD03_000858"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG4303325.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            19534..20031
                     /locus_tag="SDD03_000859"
     CDS             19534..20031
                     /locus_tag="SDD03_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG4303326.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            20216..20476
                     /locus_tag="SDD03_000860"
     CDS             20216..20476
                     /locus_tag="SDD03_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303327.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(20838..21077)
                     /locus_tag="SDD03_000861"
     CDS             complement(20838..21077)
                     /locus_tag="SDD03_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303328.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            21363..21677
                     /locus_tag="SDD03_000862"
     CDS             21363..21677
                     /locus_tag="SDD03_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303329.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="SDD03_000863"
     tmRNA           complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="SDD03_000863"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(22648..23421)
                     /locus_tag="SDD03_000864"
     CDS             complement(22648..23421)
                     /locus_tag="SDD03_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4303330.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            23565..23915
                     /locus_tag="SDD03_000865"
     CDS             23565..23915
                     /locus_tag="SDD03_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG4303331.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            23896..24612
                     /locus_tag="SDD03_000866"
     CDS             23896..24612
                     /locus_tag="SDD03_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4303332.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            24612..26060
                     /locus_tag="SDD03_000867"
     CDS             24612..26060
                     /locus_tag="SDD03_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4303333.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(26130..26639)
                     /locus_tag="SDD03_000868"
     CDS             complement(26130..26639)
                     /locus_tag="SDD03_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303334.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(26629..27354)
                     /locus_tag="SDD03_000869"
     CDS             complement(26629..27354)
                     /locus_tag="SDD03_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303335.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(27577..29697)
                     /locus_tag="SDD03_000870"
     CDS             complement(27577..29697)
                     /locus_tag="SDD03_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4303336.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            29927..31105
                     /locus_tag="SDD03_000871"
     CDS             29927..31105
                     /locus_tag="SDD03_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG4303337.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            31121..31879
                     /gene="aroD"
                     /locus_tag="SDD03_000872"
     CDS             31121..31879
                     /gene="aroD"
                     /locus_tag="SDD03_000872"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG4303338.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            31902..32387
                     /gene="ybaK"
                     /locus_tag="SDD03_000873"
     CDS             31902..32387
                     /gene="ybaK"
                     /locus_tag="SDD03_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG4303339.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(32458..33711)
                     /locus_tag="SDD03_000874"
     CDS             complement(32458..33711)
                     /locus_tag="SDD03_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4303340.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            33829..35178
                     /gene="gdhA"
                     /locus_tag="SDD03_000875"
     CDS             33829..35178
                     /gene="gdhA"
                     /locus_tag="SDD03_000875"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG4303341.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            35290..36636
                     /locus_tag="SDD03_000876"
     CDS             35290..36636
                     /locus_tag="SDD03_000876"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG4303342.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(36944..37330)
                     /locus_tag="SDD03_000877"
     CDS             complement(36944..37330)
                     /locus_tag="SDD03_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4303343.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT        12481 a         6450 c         7405 g        11021 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa aataaaatct
     3781 ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa agccccaaac
     3841 tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa aggattattt
     3901 gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa taaaatccaa
     3961 cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag aaaatactta
     4021 catcccatta tgatttcgat catcttgatt tttactttct tagggagctt atcaagtatt
     4081 tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga ggagacaaat
     4141 agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag caacaacgag
     4201 acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga aatcacagca
     4261 gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac atttagtact
     4321 gttgcggcca ctgcgaattt caacggacat accataccag atggatatac agtgatatat
     4381 gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa aactgcgtta
     4441 caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca taatctgttt
     4501 attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta tatgggggtg
     4561 gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag ttattctttt
     4621 acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt ttcaatcggt
     4681 atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg gcagatcgtt
     4741 gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt tcaggtcaat
     4801 gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa aatcggaacg
     4861 tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac tccaacagca
     4921 gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga aagtaacgtc
     4981 gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca agactatttt
     5041 ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc tgcacaagac
     5101 ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc agcgatagaa
     5161 acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg aaatgtaatc
     5221 gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa gacaaatgca
     5281 acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt tgtaccgatc
     5341 gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa tgatctcagt
     5401 attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa gggagttgaa
     5461 gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa aagcggacta
     5521 gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc tgatgtctat
     5581 acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata tgatggaaaa
     5641 gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc gcttactcca
     5701 gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc aaaaaatatg
     5761 gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa agtcaaagca
     5821 gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta taccatctat
     5881 aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc ccaaagtcac
     5941 tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc attggattat
     6001 accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat tagtttaggc
     6061 gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa gttgacgatc
     6121 aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt tgtcttttta
     6181 ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa aaaagcaggt
     6241 gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt ctctttttta
     6301 tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca cctattacat
     6361 ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt caaaacgacg
     6421 gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt actttcgcta
     6481 tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt gtggaagacg
     6541 cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa aatgtgacaa
     6601 aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa gatgagcaag
     6661 gtcgtgacgc tgtttaccga tttacggaaa taaagacttc agattaagta aaagaaaaat
     6721 cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa ttgacgacca
     6781 tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta acaaaaacag
     6841 tagagaataa gcagacagat tttgcagatg gggataaagt cccttatctc atcaccgtaa
     6901 caattcctga gaacatcaac gagataggaa cctatacgat caaagataca gctgatcctc
     6961 agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag attcatacat
     7021 ttcacacaca aaaaataaaa catggttttg tcttatcaga ttctggaaaa gatcttagtt
     7081 cgtttgctgg aaaaaaatta acgatccgtt accaaatgac attgaaagaa aacagtgaaa
     7141 aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta gaaacacatc
     7201 ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg aaacaaaaac
     7261 tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa gaaaacggga
     7321 taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc agtaaagatg
     7381 gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag attaaaccgc
     7441 ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa ggcagttatg
     7501 atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat ccgacgaata
     7561 atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa aatggaggaa
     7621 gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg ttattgctag
     7681 tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac aataggaggg
     7741 aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg ctatcatgct
     7801 gcttcctttc gttctcggcc taggaactgc agaggtttct gcagccgtat cacctacacc
     7861 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     7921 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     7981 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     8041 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     8101 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     8161 aacgtcgcca aatatcgtgt taagcttacc ctagaccctg acaaaccttt agacacgatt
     8221 cacctatatg ctaaaaatgt catcaaagaa agcaatatca aaatccagaa agctgggaac
     8281 ttttcagggg tagataagtt agcaggggcg caattccaga tcaaaaattc agagaatcaa
     8341 ttcgtgaccg gttttgatga aaacactggt gtcgctctct atgatcctgc tgagtcagcg
     8401 ttgacattca caactgatac tgaaggatcc atcagtgtca atggattaat agacggcaat
     8461 tatactttag ttgaaacaaa agcaccagat ggttatcaat taaccaatcc acaaaaagaa
     8521 actactttta tagtaaaaaa cggaaaattg caacaaacaa ccggtatcat ccatggcgca
     8581 gatgaagcca atcttgggaa tccaaacggt gtagtcaatc gggaaaatac aactacggat
     8641 aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt tccacttcat caaacaagat
     8701 gtgaacacgc aagaaaaatt agccgatgca gaattttcta ttctaagagc aaaagatcca
     8761 actgttaaat taatgaaaaa aatagcaaac cttgcctcag gagaataccc atatgcttgg
     8821 tcagatgaag tcacaggttc tgactgggaa gaagtgaaat tgatttctga ttcagaaggg
     8881 aaattttcaa tcgaagggct gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca
     8941 gaaggctacg cattacctaa gaatgaagct gcgtacactg aatttacagt agatgatgat
     9001 ccaacaactt ctgatgaaac aacgatcgat aaccaaccta aaggaatctt gccttcaact
     9061 ggtggtatgg ggatcatcgt attcatcttg gtaggtactg ctttagttgg aggagcagtg
     9121 atctatttca aaaaacgcca tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat
     9181 acgataaata aacgatagtg attcaaatgg ggagaaaggt tgtgacaagt ggtcggtctc
     9241 ttggtattcg ctcaacaaac ggcaaaatag gaggaactat tttgccgttt gcttcgctaa
     9301 tactcaggtc aaggcgctga tttcacaacc tcttcatata catattagaa aataggtgga
     9361 catgatgaat aaaagcagaa taagaatact ggacatcttc atggcaatca ttttagtcgt
     9421 tgggatcggg atcttttctt atccttttgt agaagattca ctgaatgatt ttcttgccca
     9481 gcaaatgatc atccattatc aaaagcaagc atcgaataag aattcagagg aaataaaaaa
     9541 acagcaggaa aaaatgacaa agaaaaacca acagttggca gagcaaaacg tatcaccagg
     9601 gataggcagt ttcaaccaag cagttgatgc aaaagcatta aaagatttac ctagtaatgc
     9661 cttttttatg gaacatatgc ttggagtcat cgaaatccca aaaatcaatg tcagcttacc
     9721 tatattcgat cagacaacgg aaatattttt gcaaaaagga acgtcattat tggaaggaag
     9781 ttcttatccg actggcggga aaaacaccca tgccgttctt tctgggcatc gtggactccc
     9841 agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa ggagaccagt ttttcatcca
     9901 gatcaatgga aagacattgg cgtatcaagt agaaaaaatc caagtggtct tacctgacga
     9961 agtagattct ttgggtatcc aaaaaggcag agatctcgtg actttgctta cctgcactcc
    10021 ttatatggtc aatacacatc gactgcttgt gacggggcat cgtattcctt atcaagcaaa
    10081 agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa aaatggaaat tttttatttg
    10141 gtttatcggt atactacttg gatcaatcgg cttagtttgg ctactcatcg cttatttaga
    10201 ttctttggcc atcgctaagc gaaactatcc tttatctttt tatgtaaaga acacaaacgg
    10261 acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc aacggcaaac actatattac
    10321 tagagaaaaa gttccatttg tgaaagcttc tgatgaatac ggactagtga gattttctga
    10381 tttaaaaggc aggaactatc gcttgcaaca tgaggagctc ctgcttaaaa tccatgtaaa
    10441 acataaacat tcgaaacagt tttcaatgaa attgaaaaaa ggaagatata aactgcgaaa
    10501 agaaaaagag gtgtactatt tgattgagaa agaatgaatg aagatgtcca aaaaagcaga
    10561 gggaacagac taaatgaaaa aagagagaag ctgacgaaat ggacgtgtaa actgctattt
    10621 ttggtttact atctggaacg ccagcttcta tttttttatg tttcagaaga attttattat
    10681 tatagtataa cttttgaatt agtttccata acatttggag caaggggtta aaccacgagc
    10741 ttgagcttcg cttaaagtgg caggggtata cgtaccattt ccacatttat gtgtatggta
    10801 ttttgaaccg gtcggtgtga catagacagt ttgttcatta ttttgctcag gagctgcggc
    10861 ttgtgcctgc tgagaagcct gttcagctgc tgcttgctgt cgcgcctgtt ctgcttgttg
    10921 ctgtgcgatt gcttcttgcc tcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg
    10981 cgcttgttct gcttgttgtc gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc
    11041 ttttgctttg attgtggaat caattgcagc aagacggctt gtcaactctt gtttgttttc
    11101 aggtagcgct tggatagctg ctgaagcagt tgcatagttt tcttttgtag gatttgcttc
    11161 agcttgtgac aaagcagatt ccgcttctgt cacttttttt tctagtgcag ctttttccgc
    11221 tttttgtttc tcttcttctt ttttcttttc aatttgttct ttcgatgaat ctgccgtact
    11281 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
    11341 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaacg caactaattt
    11401 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
    11461 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
    11521 tcccgtgttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
    11581 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
    11641 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
    11701 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
    11761 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
    11821 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
    11881 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaacaa
    11941 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
    12001 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
    12061 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
    12121 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
    12181 acggacgtca aagaatagtc gccgccagat agaaggaaat tagcagttga aaaagctgcc
    12241 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
    12301 gtcttattac cgcagatgca agctttttcc acacctcttt cttgtcattt acagactcaa
    12361 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
    12421 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
    12481 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataac
    12541 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
    12601 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
    12661 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
    12721 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
    12781 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
    12841 attttgcata attgaaggta gccaattcat taacccaaaa tagccggcaa tttggacagt
    12901 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
    12961 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
    13021 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
    13081 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
    13141 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
    13201 caaggacata cacgccccat attctcctcc cgctcctaca cctgcgataa agcgaaagag
    13261 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
    13321 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
    13381 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
    13441 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
    13501 ctctaaagca attcccgccg atgtcgatac taataccttt cgctgatacg ttgtcatttt
    13561 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
    13621 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
    13681 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
    13741 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
    13801 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    13861 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    13921 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    13981 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    14041 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    14101 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    14161 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    14221 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    14281 caactgaagg agttagaaga gtaactagga acagaactgt ttgtacgcga aaaccgcaaa
    14341 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    14401 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    14461 tacgatcggc tgtgtggaag ctgatagttc gaatacattg gcactattac tagaaaagtt
    14521 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    14581 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    14641 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    14701 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    14761 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    14821 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    14881 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catcattcaa agcgagtgac
    14941 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    15001 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    15061 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    15121 ttgtattttt tcatttttaa atcaaaaaaa taaagagatt tacataactt gttaaataag
    15181 cgcttacaaa aataagaaga acggaaatga atcagctata ataaccgttg ttctggattg
    15241 taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa
    15301 gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt
    15361 gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca
    15421 aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat
    15481 tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta
    15541 agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct
    15601 aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc
    15661 ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacagat
    15721 acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag
    15781 caatattaca gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca
    15841 gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg
    15901 acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct
    15961 tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca
    16021 gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt
    16081 ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag
    16141 gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg
    16201 tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct
    16261 gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt
    16321 attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaatagt
    16381 ttttcaccag tacgtttgtt cataaagatg tttcgccaat tttggctaaa gggatttttt
    16441 tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata
    16501 cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt
    16561 tcctttagca ccaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa
    16621 gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt
    16681 ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta
    16741 ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg
    16801 gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt tttatgtaaa
    16861 aactgtcatt ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct
    16921 atacttttgt atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta
    16981 ataattaaaa ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt
    17041 tcgaaatctg tagtagaaag tttgattaat catcatgttg attatgtatt cggtatacca
    17101 ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg
    17161 actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag
    17221 ccaggagtag ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta
    17281 acagcgacag cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat
    17341 ctattgaagt tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa
    17401 tacagtgcag aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt
    17461 atggctaaga gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat
    17521 gcacctgtcc aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct
    17581 gcagatgata tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta
    17641 gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag
    17701 acagcattgc ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa
    17761 gcgcatttct ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa
    17821 cgaagtgatc tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg
    17881 aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct
    17941 tttatgcagc ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca
    18001 ggttcattag agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa
    18061 gcaaaattaa cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct
    18121 ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat
    18181 gtaggtagtc attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg
    18241 ttattcagta atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca
    18301 ttggtgcgtc caaatacaca aatcgtatca gtttccggcg atgggggatt tttattctct
    18361 gcccaggatt tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat
    18421 gatggacact acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt
    18481 gtagactttg gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc
    18541 cgagctacaa gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga
    18601 cccgtaatta ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc
    18661 ttaccagatc aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac
    18721 gggacattag gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata
    18781 ttagaaaaag gaactctggg attgggaaca ttgcatgggc ttgatggtga agtcatcttt
    18841 ttagatggcg tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt
    18901 gaacttaccc cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca
    18961 aaaactgcag atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac
    19021 ctatttttgg cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg
    19081 aagcaagaaa aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa
    19141 caaagcaata tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt
    19201 gctgcagcgg gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta
    19261 atggattttg aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag
    19321 cattttccag ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca
    19381 gcagaaatcc aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct
    19441 gacgtatctc gtttttttga ggaggaaacc tagcgtttac aaaaaaactg agctataatc
    19501 ttctccatag atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat
    19561 gcatacgaaa ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta
    19621 gcaaatttag caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca
    19681 ggatattatt tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct
    19741 tgcacacgta tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca
    19801 ttgatcgttg atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa
    19861 tcagaaatcg tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat
    19921 tgcggagtaa cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat
    19981 cttttattag caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta
    20041 ttgaaacaaa aaagaagaga aatcaagaat aaaaagagcc ggaaagacag tggtcatttc
    20101 tgtttttcgg tttttttatt tttttataaa atcacaagtg ttattgattt tcacaatcat
    20161 attttaatgg tattatcatt ttgaataatg aaaaaacatt aaggatggat gaaatatgaa
    20221 taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa
    20281 agtggttgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata
    20341 tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac
    20401 tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca
    20461 tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt
    20521 gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc
    20581 gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact
    20641 gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc
    20701 aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta
    20761 aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc
    20821 tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt
    20881 ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa
    20941 tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt
    21001 atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata
    21061 aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc
    21121 tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta
    21181 gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct
    21241 gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta
    21301 ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta ggaggatagc
    21361 ttttggattt ttacagtaat tttatcttaa taatagcaat attattactt ctaaatattt
    21421 ggttttttga taagagtaga aatgcaggga taggttttag aaccaaacga agtacgtctt
    21481 ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg aggggttatt tcaatcagtt
    21541 tactttcttc aacactttat tcttttaatg ttatagacgt ttccatgtca aattttatat
    21601 ctattattgg aatccttatt tctgcaatta ttacacaact acttcttgtg tttgaagaga
    21661 agtcaaaaaa taattaaagc aattatgcct tttagtacag gaattattac ttcttacaaa
    21721 tgtatagcga atgtgaagcc tgctgtttta attgttctag aaaaactttt gagtgaacaa
    21781 ttattttatg ttaggatgaa ttaatttctc tagagtttat aattaaaaaa cttatttgaa
    21841 ggaacatgct taaaccataa ttgtgttttt ccttccatca gcataaaagt tcacctcaaa
    21901 aagagtgtac gaacaaaagt tctttttgta aaccttaaaa tagattttcc tgaatacgta
    21961 ttggggattt ctaaatgttt gaatgttttt ttgaatgcaa aatcaatgca aaaaattttg
    22021 tatttctcta cttttctctc cttgaccaaa ataaaaaaac gttgatttaa caacgttttt
    22081 catacatgtt tatctgtctc aaccatacat atatggagac ggcgggagtc gaacccgcgt
    22141 ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag gattcacttt
    22201 acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt tttcaactta
    22261 cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag cacatgggcg
    22321 atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag ttgttttcgt
    22381 ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg aaacgcaatt
    22441 caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag ggttacgtgt
    22501 atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa aagcgaatca
    22561 tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg attttcttaa
    22621 gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca atgcgtggac
    22681 aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta atccgtcata
    22741 ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat cattcggacc
    22801 atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca gacgtttgac
    22861 acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac cattccgata
    22921 aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag ataagatcag
    22981 caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa catctgaatg
    23041 aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga ttttctgatc
    23101 gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt tttgacattc
    23161 ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga aagaaccatt
    23221 catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt ctgcttctgt
    23281 acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca tagcttttgc
    23341 cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat caaagaaagt
    23401 gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag tttatcttaa
    23461 caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt ggttcagtca
    23521 acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt tttttcagta
    23581 ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca aaaggaaatc
    23641 tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat tggcggctgg
    23701 ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca ttgatcggct
    23761 attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt ctgctagtat
    23821 ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat gacctggaaa
    23881 ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt ttctgaaatg
    23941 atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga tggaaaagaa
    24001 gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact agacctgaat
    24061 ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc aagtactgtg
    24121 ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg cttagagatg
    24181 ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc tcgaatgaaa
    24241 gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagcagt ttcagatgat
    24301 cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga agcgtatctg
    24361 gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt tactttagca
    24421 aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg ggattatcag
    24481 tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca aaaaatcgaa
    24541 aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt cgatgattct
    24601 ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttttgcgga atggtcgcgt
    24661 tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg attgaagaaa
    24721 acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg caaacctatg
    24781 ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg ctgctgacag
    24841 agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac gaacgtgtca
    24901 tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa tggaaaaatc
    24961 tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga caagcacagg
    25021 cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga gcacttgtag
    25081 ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg aacctgttca
    25141 aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt gcagcattcc
    25201 aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat ggtaattttg
    25261 atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat gatttcaata
    25321 aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa aatcgtcgaa
    25381 gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca atcaacggac
    25441 ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac gcgattaaat
    25501 tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg gattatgaaa
    25561 aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca gaaacattga
    25621 aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca ttgacattga
    25681 aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa atcatggtca
    25741 atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt actttagaaa
    25801 aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg tctgaacaac
    25861 aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa aatacaaaat
    25921 atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg cataaaggaa
    25981 aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt ttgcctgacg
    26041 tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg atcttctata
    26101 ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg atcaatataa
    26161 ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt ttcttcatga
    26221 gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag ttcatcaaat
    26281 gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt
    26341 actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg agagatatca
    26401 agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac atccaacagg
    26461 acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat gatcgcaaaa
    26521 ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt atgctgtttt
    26581 tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt
    26641 acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga atagactaag
    26701 aatgagacag catccactga gcccaataag agtttgggca aatcccttaa gtatatctgt
    26761 gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt
    26821 aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag tctttaagac
    26881 aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta cgatccacaa
    26941 taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa
    27001 taatttggtt ttagaaagac cagtcaggcg ccaattgatc aattcctgct tgttttgcct
    27061 gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata aactgaatat
    27121 gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg tagatgcaga
    27181 agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt gcaaatggta
    27241 aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac aatagagtat
    27301 tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt tcatactttt
    27361 cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc tttagtgaaa
    27421 tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag aagctgccct
    27481 attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag gagcagcgac
    27541 aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac ttgtggtcgt
    27601 tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg
    27661 ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc
    27721 taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata
    27781 gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt
    27841 ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc
    27901 agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc
    27961 atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat
    28021 ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc
    28081 gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac
    28141 acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc
    28201 agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac
    28261 tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc
    28321 taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg
    28381 gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa
    28441 ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg
    28501 tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc
    28561 aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc
    28621 gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc
    28681 ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa
    28741 tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa
    28801 gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc
    28861 atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc
    28921 taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt catttggtgc
    28981 tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc
    29041 ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc
    29101 aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc
    29161 ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc
    29221 cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt
    29281 caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact
    29341 ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc
    29401 cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat
    29461 gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa
    29521 taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat
    29581 tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag
    29641 taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat
    29701 aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt acacagctca
    29761 cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat
    29821 gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta
    29881 tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt
    29941 aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct
    30001 tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt
    30061 tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca
    30121 gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag
    30181 aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga
    30241 cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa
    30301 cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga
    30361 aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt
    30421 catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc
    30481 cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg
    30541 atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg
    30601 cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc
    30661 aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca
    30721 gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt
    30781 tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt
    30841 tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga
    30901 cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat
    30961 gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct
    31021 gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt
    31081 ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg taaagggatt
    31141 gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga
    31201 agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt
    31261 cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca
    31321 tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga
    31381 acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt
    31441 agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga
    31501 ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc
    31561 agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg
    31621 aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag
    31681 acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa atcttggtaa
    31741 aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag
    31801 cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt
    31861 attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag
    31921 taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat
    31981 atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc
    32041 aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg
    32101 tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa
    32161 aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct
    32221 gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct
    32281 atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag
    32341 atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa
    32401 aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta
    32461 tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt
    32521 aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat
    32581 gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac
    32641 aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg
    32701 aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag
    32761 ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa
    32821 gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg
    32881 tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt
    32941 gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa
    33001 atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc
    33061 ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg
    33121 ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc
    33181 atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg
    33241 agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa
    33301 tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa
    33361 tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc
    33421 ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc
    33481 aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg
    33541 acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt
    33601 tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat
    33661 catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc
    33721 tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt tgagccaaaa
    33781 gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat gacaaacgca
    33841 acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa
    33901 tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga cactcatccc
    33961 gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt tattcaattt
    34021 cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta ccgtatccaa
    34081 tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag tgtcaatcta
    34141 agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac cggtctgcca
    34201 atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga taatgagatc
    34261 atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc atcgattgat
    34321 gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt cggcgaatat
    34381 aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga tttttgggga
    34441 agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa acatctattg
    34501 aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag cgggaatgta
    34561 gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac atgttcagat
    34621 tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa agaagtcaaa
    34681 gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc tatttatcat
    34741 gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc atgtgctact
    34801 caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat caaaatcgta
    34861 gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca tgaagcaggc
    34921 gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc tgctttagaa
    34981 atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga gcagctggat
    35041 caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta cggcgacgaa
    35101 aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa agcaatggca
    35161 gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca ttttgtttat
    35221 ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa cctataagga
    35281 ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa
    35341 caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt acgggaaggt
    35401 actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta tgacaaagaa
    35461 gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga agtattagtt
    35521 gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt cttgaatcac
    35581 acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt ctttgccgga
    35641 aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga tcgcgacttt
    35701 tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc gttccgtgta
    35761 ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg tatctacgct
    35821 acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg ttgggaaact
    35881 ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt tggtttgtta
    35941 ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc tgatgcaagc
    36001 aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc agcaatgcgc
    36061 aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga accaggaatg
    36121 caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa
    36181 ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg acaattcatc
    36241 caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca
    36301 atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca aggaaaagaa
    36361 gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac agacggtgac
    36421 gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg ttatacaatg
    36481 tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa cccattcgac
    36541 caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa accaggcttt
    36601 gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc gctagattta
    36661 aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact
    36721 caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt gtcacagtcc
    36781 cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag gactttaaga
    36841 acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat cagtaagcaa
    36901 aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat aggcctaatt
    36961 tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct atgatccgct
    37021 tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca ttggcagagt
    37081 caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca gcataagcat
    37141 ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg atatcagttt
    37201 ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa gtagactgat
    37261 agtagtccca acctttccat cctataaaag caatcaatat gataacgatc aaccctaaaa
    37321 tttttttcat gaaagatctc cttttttatt tttatta
//
LOCUS       DARDHU010000016        37220 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000016 DARDHU010000000
VERSION     DARDHU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.016"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            156..1157
                     /gene="ccpA"
                     /locus_tag="SDD03_000878"
     CDS             156..1157
                     /gene="ccpA"
                     /locus_tag="SDD03_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG4303344.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1494..3839)
                     /locus_tag="SDD03_000879"
     CDS             complement(1494..3839)
                     /locus_tag="SDD03_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG4303345.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            4118..5020
                     /locus_tag="SDD03_000880"
     CDS             4118..5020
                     /locus_tag="SDD03_000880"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG4303346.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            5233..7008
                     /locus_tag="SDD03_000881"
     CDS             5233..7008
                     /locus_tag="SDD03_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG4303347.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(7074..8660)
                     /locus_tag="SDD03_000882"
     CDS             complement(7074..8660)
                     /locus_tag="SDD03_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG4303348.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8911..9135
                     /locus_tag="SDD03_000883"
     CDS             8911..9135
                     /locus_tag="SDD03_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG4303349.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9277..11028
                     /locus_tag="SDD03_000884"
     CDS             9277..11028
                     /locus_tag="SDD03_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4303350.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            11028..12812
                     /locus_tag="SDD03_000885"
     CDS             11028..12812
                     /locus_tag="SDD03_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303351.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            13138..13989
                     /locus_tag="SDD03_000886"
     CDS             13138..13989
                     /locus_tag="SDD03_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4303352.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            14043..15794
                     /locus_tag="SDD03_000887"
     CDS             14043..15794
                     /locus_tag="SDD03_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4303353.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            15798..15968
                     /locus_tag="SDD03_000888"
     CDS             15798..15968
                     /locus_tag="SDD03_000888"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4303354.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(16008..16910)
                     /locus_tag="SDD03_000889"
     CDS             complement(16008..16910)
                     /locus_tag="SDD03_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4303355.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            17141..18544
                     /locus_tag="SDD03_000890"
     CDS             17141..18544
                     /locus_tag="SDD03_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4303356.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            18544..19977
                     /locus_tag="SDD03_000891"
     CDS             18544..19977
                     /locus_tag="SDD03_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4303357.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            20122..21048
                     /gene="trxB"
                     /locus_tag="SDD03_000892"
     CDS             20122..21048
                     /gene="trxB"
                     /locus_tag="SDD03_000892"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG4303358.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            21262..22989
                     /locus_tag="SDD03_000893"
     CDS             21262..22989
                     /locus_tag="SDD03_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG4303359.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            23004..23354
                     /locus_tag="SDD03_000894"
     CDS             23004..23354
                     /locus_tag="SDD03_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG4303360.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(23446..24306)
                     /locus_tag="SDD03_000895"
     CDS             complement(23446..24306)
                     /locus_tag="SDD03_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG4303361.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            24398..24826
                     /locus_tag="SDD03_000896"
     CDS             24398..24826
                     /locus_tag="SDD03_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4303362.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24988..25164
                     /locus_tag="SDD03_000897"
     CDS             24988..25164
                     /locus_tag="SDD03_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG4303363.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            25191..25637
                     /locus_tag="SDD03_000898"
     CDS             25191..25637
                     /locus_tag="SDD03_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG4303364.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(25791..27170)
                     /gene="brnQ"
                     /locus_tag="SDD03_000899"
     CDS             complement(25791..27170)
                     /gene="brnQ"
                     /locus_tag="SDD03_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG4303365.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            27876..28847
                     /locus_tag="SDD03_000900"
     CDS             27876..28847
                     /locus_tag="SDD03_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG4303366.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            28871..31063
                     /locus_tag="SDD03_000901"
     CDS             28871..31063
                     /locus_tag="SDD03_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG4303367.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            31080..31556
                     /gene="ybeY"
                     /locus_tag="SDD03_000902"
     CDS             31080..31556
                     /gene="ybeY"
                     /locus_tag="SDD03_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG4303368.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            31540..31935
                     /locus_tag="SDD03_000903"
     CDS             31540..31935
                     /locus_tag="SDD03_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG4303369.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            31950..32849
                     /gene="era"
                     /locus_tag="SDD03_000904"
     CDS             31950..32849
                     /gene="era"
                     /locus_tag="SDD03_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG4303370.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            32992..33804
                     /gene="recO"
                     /locus_tag="SDD03_000905"
     CDS             32992..33804
                     /gene="recO"
                     /locus_tag="SDD03_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG4303371.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            34188..35105
                     /gene="glyQ"
                     /locus_tag="SDD03_000906"
     CDS             34188..35105
                     /gene="glyQ"
                     /locus_tag="SDD03_000906"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG4303372.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            35107..37182
                     /locus_tag="SDD03_000907"
     CDS             35107..37182
                     /locus_tag="SDD03_000907"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG4303373.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        12113 a         6644 c         7875 g        10588 t
ORIGIN      
        1 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
       61 aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt
      121 cagaaaacat ataaaatatg aataggagaa cacaaatgga aaagcaaaca atcactatct
      181 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
      241 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agttatcgat cgcttggatt
      301 atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggtgtaa
      361 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
      421 caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag
      481 tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc
      541 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
      601 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
      661 aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
      721 cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
      781 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
      841 gtctatctct agtcgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
      901 atgaattagc catcggtatt ttggatggtt tattagaccg tggcgtgaaa gtaccggaag
      961 aattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     1021 caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa
     1081 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     1141 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgtgcctg ccgtttaact
     1201 ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg tttgatgaat attgtgactc
     1261 gtgattcgga gttggcagga gaacaccgtt tatttgggaa aaggggttat gacaaaattt
     1321 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     1381 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
     1441 ggactgtgac aatactattg tcacaatccc ttattactgt gtattctatt aaatcaatca
     1501 tctttctcat ctgattcttt agttgcttga ttcgctcttg cataagctga agcggtgttc
     1561 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
     1621 ggtccatttt tcgcccatag cgattcaact gtttttcctg gtgtttggat ggatttttta
     1681 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
     1741 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
     1801 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
     1861 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
     1921 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
     1981 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
     2041 ctacgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
     2101 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
     2161 tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt tggtctgttg aagcgtcgtg
     2221 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
     2281 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     2341 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     2401 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     2461 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     2521 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     2581 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     2641 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     2701 atgtatccat aattttcaac gccattttgc aatgcagcat agacatcttt atcgattgtc
     2761 gaatggattg tatatccttt attttggatc gtttgctgtg ccttttgata ataagcgtca
     2821 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     2881 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     2941 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     3001 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     3061 gaaagatctt cttttagcgc gcctgtgttc gtgtatggac tatacgtgat cgggctttgg
     3121 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     3181 attccttttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     3241 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     3301 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     3361 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     3421 gatactaatg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     3481 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa tcttatcaga tgaaatagtc
     3541 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     3601 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     3661 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     3721 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     3781 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     3841 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     3901 cagacaaggc tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg
     3961 atctttatta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     4021 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     4081 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     4141 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     4201 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     4261 gaatattgat gccggaaaag cgtacatggc agaaaaaggt ctaacaaata tcgtcgttca
     4321 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     4381 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     4441 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     4501 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     4561 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     4621 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     4681 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     4741 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca
     4801 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     4861 aatgtttgac aatatttcga aaatcttgga aactccctat gtaggcaaag ataaaaagaa
     4921 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcgcaaacat tcgatccaga
     4981 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     5041 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtacttt
     5101 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacaaggt
     5161 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     5221 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     5281 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     5341 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     5401 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     5461 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     5521 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatt
     5581 gtgaagaaca agtatcttct tttaagtatc tatttttatt ttttgttttc tccgattagt
     5641 tttacacatg aatattccac tcgaacatat cgtaatgccc tcgtgattcc ggctgttgag
     5701 atcattatcg cttgtttatt agctatttat taccgcaaat acggttctag cagaaaattg
     5761 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     5821 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     5881 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     5941 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     6001 gtctatcaat ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     6061 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     6121 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     6181 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     6241 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     6301 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     6361 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaacaat caaaaagaaa
     6421 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct gccaatcgta
     6481 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     6541 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     6601 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     6661 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     6721 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     6781 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     6841 gtttttcttt tcggtgtctc atggttttgt tcttatgcgc cagaaaaaaa ggactatttt
     6901 ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg
     6961 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     7021 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     7081 atttcttcta ccgtttcctg caaaagctgt tcatattttt tgtttccttt atataaatca
     7141 ccaatcgtcc atgccaacag gagaaggaca agaacaatga ttacataagc gatgtgatca
     7201 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     7261 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     7321 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     7381 ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     7441 ttcaaagcgg catgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     7501 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     7561 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     7621 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     7681 atcaaaatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     7741 gaaggatctt ggacagaacc ggttttgaag acagcgacac ccatgatcaa tagcagtgca
     7801 ttgactagaa aagccattga caactggata ttagaatccc aagtagaaaa acgtacagct
     7861 tgtgctacat cttcttcgtt ttggtgatcg atcttccgag tttgtgaaac tgctgaatga
     7921 agatataagt tatgcggcat gactgttgca ccaataatcc ctaaagctcc agtcagcgga
     7981 gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac tgttagggat aaagccagca
     8041 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     8101 acgaaaatta agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt
     8161 aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     8221 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     8281 aattcagtca agatccataa aatgacacct aatgtcttac ttgtgcgtgc tcggattgcc
     8341 tgtgccaaat ccatttggct cacgattcct aattttgctg ccatatactg taaaagcata
     8401 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     8461 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     8521 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc aactgttcca
     8581 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     8641 ttattggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     8701 cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg cctaattttt tttcatggcg
     8761 cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca
     8821 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga
     8881 gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt
     8941 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
     9001 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
     9061 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
     9121 caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt
     9181 ttttatttcg taatttgtta gactattcag attattcagc aaaaaagaca agcacagaca
     9241 atcctaggct cttgataaag acaaaggagg agtaaaatgt cgatttttag aaaattaggc
     9301 tggttttttc gtcaggaaaa gaaacattat ttaattgggg ttttctcgct attcgcagtg
     9361 gccttggtac agttagttcc tccaaaagtg attgggatca tcattgatga gatcgccgat
     9421 aaaaatttat caataaagat cattttcggg tggatcttcc tattgattat cgctgctttt
     9481 ttacaatatc tttttcgtta tatctggcgg acgaatattt ggggaagtgc agctcgattg
     9541 gagaaagaac ttcggcagca gttattcaaa cattttacag aaatggacaa tctcttttac
     9601 caaaaatatc ggactgggga tttgatggct cacgcaacga atgatttgaa tgcgatccag
     9661 aatgtagcag gagctggtat cttgaccttt gcagattcag tgattacggg cggaacaacg
     9721 atcattgcga tgattttgtt tattgattgg cgcttaacac tgattgcgct gattcctctg
     9781 cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac tccatgatgc ttttcgtgat
     9841 tcgcaagaag ctttttctag tatcaatgat aagacacaag aaagtatcac tgggatcaaa
     9901 gtcattaaaa catttggaca agaaaaagaa gatctagcgg attttgaaga aaagattgaa
     9961 gatgcaatta ttaaaaacaa acgagtcaat tttttagact ccttatttga tccattcatt
    10021 accttgatta tcggtctgtc ttatgttgca acaattattt taggtggaaa gtatgtcttg
    10081 gaaggcacga tctcgatcgg gcagatggtc tcatttatta gttatatcgg tatgcttgtt
    10141 tggccgatgt ttgcgattgg tcgattgttc aacgtattgg aaagaggaaa tgcttcttat
    10201 gatcgcgtca atgaactttt gcatgaaaag acgcatatcg tggaacaact gggtgcagtg
    10261 cagaccccgg caaaaggcga gctgaatgta gctatttcct cttttaatta tccgaaagaa
    10321 gaatcaccag cattagaaca aatcaagttt gcgatccaag aaggagaaac attaggtatt
    10381 gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat tactgatgcg tgaatacgat
    10441 gattatcaag ggaaaatcca atttggcgga cgttcgatca aagaatattc attgaatgct
    10501 ttattaggat caatcggcta tgtgcctcag gatcacttct tgttttcgat gaccattcga
    10561 gataatatcc gtttttctaa tccaaaactt tcagaagaag aagtagagca tgcggcttct
    10621 ttagcattta tcaacaatga aatcaaggaa ttaccaaaag gttatgatac cttagttgga
    10681 gaacgtggag tctctttatc tggcggacag aaacaaagga tttcgattgc tcgagcattg
    10741 atcaaaaatc ctgaattatt aatattagat gatgcactct ctgcagtaga tgccaaaaca
    10801 gaagaagcta tcttgcataa tttaaaagaa ataagaaaag agaaaacaac tatcattgca
    10861 gctcaccgtt taagtagcgt gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa
    10921 atcattgaac gaggaaccca tcatgagctg cttgcactag gcggctggta tgaacaaatg
    10981 tgggagaaac agcaattaga agcgaagata gaaggaggcg aggcctgatg gatgaaaatt
    11041 atcaatcgga atggacaaaa tcgattccac ttaaagaaca gaaaaagatc atcaaacgac
    11101 tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt tgctatctta tttgcgtttg
    11161 ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca aacttttatg gatgatcatc
    11221 tggctaaaca gtcggctacg acacaagtta ttttgttttt tgctggtatg tacttatttg
    11281 gagtcatagt aaaaagtatc atttggtttt tccaatggta tttgtattcc atggcctcat
    11341 tgaagaccta tcagtatatt agggtaaaat tatttgaaaa gctgcataca ctaggtatgc
    11401 gttattttga tcagacaccg gcaggatcaa ccgtttctcg agtgacgaat gacacagaaa
    11461 cgctatttga attttggtac gtctttttga tggtattgac tgggatcttt gcagtcgttt
    11521 cttcattcct tgccatgttt caaatcaatg gagaaattgc cttgtggact ttggtgtttt
    11581 tgccgatttt aggaatcgtc atctggtatt accagaaatt cagttctcgt atttaccgaa
    11641 atatgcggga aaaattaagt cagctgaaca cgaaattgaa tgaatcgatt tccggcatgc
    11701 gtattatcca gcagtttcgc caagaagctc gtttagctaa agaatttgaa caagtgaaca
    11761 atgagtattt agacacgcgt tatgcgatga tcaaaacgaa ttcgattctg ttaagtccga
    11821 tcatcaattt tctatatgca ttggcgattg cgttggtgtt gactttgttt gggatcgatg
    11881 cgttgcattc tcccgtagaa gttggactga tctatgcatt tacgacctat gtccagggat
    11941 tttttaatcc aatgtctcaa atgatggact ttttgagcat cttcacagat gggatggttg
    12001 ctggtagtcg tatcttgaag attatggata cggaagaata tgcaccacaa caaaaccctg
    12061 gagcagatca aatgattact tcaggtaaga ttgagtttcg caatgtaagc ttttcttatg
    12121 atggagagca caatgtatta aacaatatct cttttgttgc taatccggga gagaccgttg
    12181 cattagtggg acacaccgga agcggaaaaa gctcgatcat caacgtattg atgcggtttt
    12241 acgaatttta tgaaggtcag atattgattg atgatcatga cattcgagaa tatccaatgg
    12301 aagagttacg taagaaaatg gggctggtct tgcaagatgc tttcttgttt tatggggata
    12361 tcgcaggaaa tattcggatg atgaatccag ctatcacaga cgaacagata cgccatgcgg
    12421 ctgagtttgt tcaagcaaac aaattcatcg atgaattacc aaaaggatat cactcaaaag
    12481 tgatcgaaca tggtgccagc tattctagtg gacaacgaca gctgatcact tttgcgcgaa
    12541 caatcgtgac tgatcctaaa attctagtat tggatgaagc aacagcaaat atcgatactg
    12601 aaacagaagg attgatccaa gaaggattgg caaaaatgcg tcaaggacgg acaacgattg
    12661 cgattgccca tcggctgtca acgattcgag atgcagaatt gattttagtt ttagacaaag
    12721 gtcatatagt agaacgcggg aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag
    12781 atatgtacaa actgcaaagc agtggtatat aaaagatgtt gtgacagtag aattttgacc
    12841 taagcactag agtgaaaata aaacatagct tctcatattt ttcatctgtg agagttagtg
    12901 tcgtagtcac cagatttaaa aaaagattcg acagctagtc agaagtgcct aatagccact
    12961 aagaaagcgt tgacaaaagc atttgctttc gataatataa agaaaacgat acaacttgga
    13021 tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggtttcat ggacggcttt
    13081 ttgggttttt ctttttgttt tggatacagc cctatacatc atggaaagga gcaaaacatg
    13141 ctgattgaga aagtactgaa taacaacgtc gtaataacca gggatgaaag aaatcaggaa
    13201 atgatcgtga tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc gatttttgac
    13261 tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc tttcacaaca gttccaagaa
    13321 ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat tgaatataca
    13381 aaagaggctc tacaagtcca gttgaatgat tctgtgtata ttgcattgac ggatcatttg
    13441 tatacggcta tcgaacgtca caaaatggga atatttgtac caaatgtatt gctgtatgat
    13501 atcaaacaac tatttcctaa agagtatgcc atcgggaaaa aaacagtgaa gaaaatctcg
    13561 gataaatacc acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc
    13621 aatgcacaag gcattcaaag ccagcaagac aatacctacc tgtttactat aacaatacag
    13681 gatattctaa atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat
    13741 ttttttcgat tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa
    13801 tcagaagtgg gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat
    13861 acgtttggtt gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg
    13921 tccacggacg aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt
    13981 tgtaaataaa aaaaggagga taaattaaca agaacgtatg aaataagggt agtgacattc
    14041 agttggctaa accttccact aataaattgg agggaataga aatgggaaaa taccaagaat
    14101 tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa tatcaatggt ttgacacatt
    14161 gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa agcaaatgat gagatcttga
    14221 aaaatatgga cggtattgta acggtaatgc gagcgggagg gcagtatcaa gttgtcattg
    14281 ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc aggaaacctt acttttgatg
    14341 aggctgtttc aacagaaaaa atgagaccgt ttgatatgct tattgatgga atatcgggct
    14401 gtttccagcc attccttgct atcctagctg ctggcgggat gattcgtgga ctgacagcat
    14461 tccttgtctt cttaggcgcg tttgaccgcg gttctggcac atttgttatg tttgataata
    14521 tcggtgattc tgtattccaa tttatgcctg ttattatcgg tttaacagct gcccgtaaat
    14581 ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc attaatgaat cctagcttgt
    14641 cgctggaagc tttgagtggt gcagcagaag caccactaac gaccatattt tctggcacta
    14701 tttttgaagc acctatttat caaacagtat tcggcattcc ctggattgcg cgtaactacg
    14761 cttcttctgt cattcccatt atctttattg tgttgttagc tagccaaatt cagaaaccaa
    14821 ttaaaaagtt agtacctgag atgatcgcga atttcttcgt tccattcttc accgttctta
    14881 ttacaatgcc tttaggtttt ttactcgttg gaccagtatt cacattcgca actgatattt
    14941 taatggctgg atttgaaaca cttttagcac tttcgccagt tatttatgga gcaatcgtag
    15001 gcttcttctg gcaaattctg gtgatgtttg gtctccattg ggcaatcgta ccaatgggat
    15061 tgatgcaatt ttctgtgaat ggttggcaaa atattatgac acctgttgca gtagtgtctt
    15121 ttggacaaac tgcagcactt accgcactct actttaagct tagaaatcca aaagacaaag
    15181 caattgcaat accagccatt gtttctggta ttgtggggat tacagagcca gctatttatg
    15241 ggtttacatt accacgtaaa aaaatattta tttttacctg tgtcggtggt gcgttagggg
    15301 gcgcgtatag tgggttaatg aatttaactt cttggaatca aggtggacta ggcatcttta
    15361 caattcccaa ctatattaga ccagatggtg atttaacaga tgtgattaat gtattgattg
    15421 ggatcgccat tgcgatggtg gtatccttta cgctgacttt tttcttttgg aaggatgaag
    15481 caggtgaaac agatgatata cagaaaaaat caggtaaaga aattgtaaaa acaccgatac
    15541 aaggacaaat cgctccacta aatgcagcaa aggatgcagc ttttgcacaa ggaacactag
    15601 gtcgaggaat cttgatctac cctgaaaaag gagaagtacg tgcccctttt gacggaacag
    15661 tcatgaccct gttcccgacg aaacaggcaa ttggaatggt ttctgaaaca gggctggaat
    15721 tgctgattca tgtcggattg gatactgtgc agttggaagg gaaatatttt gaaagcttag
    15781 tgcagcaagg ataaagagtg aaacaaggcg atctgttgct acgcttcgac aaaaagaaaa
    15841 tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt caccaattac acggattatt
    15901 tggatatttt ggaaaaccaa ggggagacgg tcgggccaga cgatgcactt atcaccgcac
    15961 tgacctgaac ggttaggtga agaatggact aggcttaact attcttttta ttccacttct
    16021 ttattttcaa aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcgaat
    16081 ttttgaccga cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa
    16141 ctatataaca cgtctagaag ataacaaatt gatgtattga tcgtaaaatt accaattttt
    16201 gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg tactcattgc agatagcgtt
    16261 gaatcaccga tactggtcaa agctaaaatt ggaacatgat tttgtttcaa tatttctgcg
    16321 gtacgaagga tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc
    16381 gtgcaattgt acgcttcata gaatgattca ccaaaagtgg tgacaatcgt gacatccatt
    16441 ttgatgcgct tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg
    16501 aaaatcacga ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga
    16561 tgaatgagag aaagtgtatc atgaatcgtc atctgttcta aagaagccat tttatttgcg
    16621 atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc
    16681 ccttcaagat aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa
    16741 cccatttttt ttgctacacg gatgagtgta gaggggtgga cataacatcg ctcagcaatc
    16801 gcttggatgg tcatttcgcc aagtgctgga gaatcgctta agagaaactg gatcacttca
    16861 ttttctgaag gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct
    16921 tcttcttatt tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat
    16981 actatataac ttaaaataga cattttttga caagatattt acaaaaatcc actagaaaac
    17041 agtaaatttt gtaaatgtta ctggaaagaa acagatttct attgtttgtt ttgtttacaa
    17101 aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac atggcaaaag gaatcaaaat
    17161 cgctacgatt ggtggaggca gcagttacac ccccgagttg atggaaggat ttatcaaaag
    17221 atatgaggaa ttaccgatac gagaaatctg gctcgtagat gtagaagaag gaaaagaaaa
    17281 gcttgagatc gttagtcaga tgtctcagcg tatgtgggat gcttctccgt atgaagtcaa
    17341 gatctatgca actctggatc gggaagaagc attgaaagat gcggattttg ttacgactca
    17401 gtttcgtgtc ggtcaattag atgcacgaat caaagacgag cggatcccta gttattatgg
    17461 gatgattggg caagaaacaa atggtgctgg cgggatattc aaagcttttc gtaccgttcc
    17521 agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt ccaaatgctt ggctgatcaa
    17581 tttcgcgaac ccaagtggga tggtgacaga agcagttatc agatatggca aatgggataa
    17641 agtgattgga ttatgcaatg tgccagtgat ggcgatgatg acagaaccag caatgcttgg
    17701 caagaaaaaa gaagagctga tctacaagtt tgccggtcta aatcatttcc attggcacaa
    17761 agtagcagat aataaaggaa atgaccgcac ggcagaattg attgatcgtt tatacgatga
    17821 agagaatgga ttaccgaaaa atatctttga tatcccgttt ttcaaagaac aattaaaaca
    17881 aatgaacatg atcccttgtg gctaccaccg ctattactat cgtgaagaag aaatgctgga
    17941 acatgcttta gaagaatatc aaacgattgg tacaagagcg cagcaagtga aagaaacaga
    18001 agcagagcta tttgaattat acaaagatcc agaattagac cataaacctg aacaattgca
    18061 aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa acgattgctt caatctatgg
    18121 aaataaagaa acacagatcg tggtatctac aaagaatgat ggagctgttc ctgacctgcc
    18181 ggcagattgt gtagtagaag tcactgccta tgttggcgca caaggagcga gaaatgtagc
    18241 gtttggttct cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga atatggaatt
    18301 gctgacgatc gaagcagctg tgactggtga ttatgggaca gctttacagg cgtttacgat
    18361 caatccactt atacgatcag gaaagacagc aaaacggatc atggatgagt tgtttattgc
    18421 acaccagcag tatttaccgc aattcaagga gacaatcagc cgattaaaag aagaaggaat
    18481 cgaagttcaa gatgaactgg caagagaatt atcaggtaat acacatataa tggaggaagt
    18541 ataatgggat tcagaaaaga ttttttatgg ggcggtgcta cagcagccaa tcaatgtgag
    18601 ggcggttatg atgaaggtgg acgcggacta gcaaacgtag atgttgtgcc aatcggaaaa
    18661 gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg aatttgatga ggatcatttt
    18721 tatccagcaa aaattgcaat cgatatgtat catcgttata aagaagatat tgctttgttt
    18781 gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct ggagtcgtat ttttccaatg
    18841 ggggatgaga aagagccgaa tgaagctggc ttgaaatttt atgaggatgc ctttaaagaa
    18901 tgccaaaagc acggaattga gccacttgtg acgattacgc attttgattg tccaatgcat
    18961 ttgatcaaag aatatggtgc gtggcgttcc agagaactcg ttggttttta tgaaaatctg
    19021 tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat attggctgac attcaatgag
    19081 atcaatatga tccttcatgc accattcatg ggcgcagggc tgtattttga aaaaggagaa
    19141 aatgaagaac aaatcaaata tcaagcggca catcatgagt tggttgcaag tgccatcgca
    19201 acgaagattg ctcatgaagt agatccggag aacaaagtag gctgtatgct ggcagcagga
    19261 gcatattatc catacacttg caatcctgaa gatgtttggg cttctagaaa atcagatcga
    19321 gaaaattatt tcttcattga tgtccagtca cgaggagaat atcccgctta tgcattgaaa
    19381 gaaatggagc gaaatggcat tcagatcgaa atggaaccag gggatgccgc attgctgaaa
    19441 gaacatactg ttgattttat ctctttttct tattatgctt cccgagtttc gactaccgac
    19501 aacgaactct tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa tccatatctg
    19561 caagcaagtg aatgggggtg gcaaattgat ccattaggct tgagaataac gatgaatgat
    19621 atttatgatc gttatcaaaa accattgttt attgtagaaa acggcttagg tgcagtcgat
    19681 acgccagatg aaaatggtta tgtggaagat gattaccgaa tcgattacct ggcagcgcat
    19741 atccaggcga tgaaggatgc agtagaacta gacggagtcg atctattagg ctatacgact
    19801 tggggatgta ttgatttagt atcagctggt accggtgaga tgaagaaaag gtacggattc
    19861 atttatgtcg atcgtgataa ccaaggaaat ggcacattag cccgttcaaa gaaaaaatca
    19921 ttcaactggt ataaaaaagt aattgattca aatggagaaa atctttcgaa cgattgatca
    19981 atgatttata aaagtctatt gtttttcagc ggatttttcg agtataataa agctattcga
    20041 gaaacgcaag cagaacaata gacttttttt cgcattggct gtaaagtatg cttacttgat
    20101 taagtaaagg agaatctaca tatgtatgat gtaatcgtta ttggcgcagg tcctgctggg
    20161 atgactgctg ctttatatgc ttcacgttca aacttatccg tattgatgat cgaacaaggt
    20221 gcccctggcg gccaaatgaa caatacggca gaaatcgaaa attatcctgg attcgattcg
    20281 atcatgggcc ccgatctagc ttataagatg tatgaaagtg tctcaagatt tgggacagaa
    20341 aatgcttatg gaatcgttca agatatcaag gatcacggca gccataaaga agtcatctgt
    20401 gaagataaaa gttacgaagg aaaaacagtc attatcgcaa caggatgtat ccaccgtaaa
    20461 ttaggcgtac ctggcgagga agaatttgct ggacgaggcg tttcttactg tgcagtctgt
    20521 gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg ggggtgggga ctcagcagta
    20581 gaagaagcca tctatttgac acaatttgct tctgaagtag tgatcgtcca ccgtagagat
    20641 gaattacgtg cacaaaagat catccaagac cgtgcatttg caaatgaaaa aatttccttt
    20701 atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa tggtagtgac aggtgtgaaa
    20761 gcacgtaatg tcaaaaccgg caatgaattt gatttagcag cagacggagt attcatctat
    20821 gttggactgg atccattaac tgcacctttt agagaagcag gtttgacgaa cgaagctggc
    20881 tggattccaa cagacaacga aatgaaaacg aatatgccag gcgtttttgc catcggagat
    20941 gttcgtgaaa aagatttgcg acaaatcact acagctgttg gtgacggcgg tattgctgga
    21001 caacaagtgt ataaatactt ggagtcttta gaagaaacaa cagcataagt ttaaaaagag
    21061 tagaagaacg ggttcaattg ttcttttact cttttttttc gcgtgaaatt tactgaatct
    21121 gatttatgcg aacacgtcca acaccttttg ataaaagaat cctttcttga tttaagcgtt
    21181 ttttggtcct catactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt
    21241 tcagaaagag gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa
    21301 gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa
    21361 cttgaagatg ctttttatgc accgttggaa ttcggtactg caggtatgcg gggaatctta
    21421 ggacctggga tcaatcgaat gaacatctat acggtaagac aagcaacaga aggactagca
    21481 cgtttcatgg atacacaagg tgaagaaaca aaacgccgag gtgtcgctat cgcttatgac
    21541 tcacgccatc agtcaccaga atttgcaatg gaagcagcca aaacactagc acatcacaat
    21601 atcccgtctt atgtattcga aagtctgcgt ccaacaccag agctttcttt tgcagttcgt
    21661 catttgaaga cgtttgcggg aatcatggta accgcatctc acaacccagc ggcttacaat
    21721 ggatataaag tctacggaga agatggcgga caaatgcctc ctgcagacgc agatgcattg
    21781 acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg aagtattatc tgacgaagaa
    21841 gctaaacata gcgggttgat cacgatcatc ggagaagaag tggacgctgc ttatctagaa
    21901 gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa caatggggaa agatttgaaa
    21961 cttgtgtaca cgccacttca tggaacaggg aaaatgttgg gcgaacgtgc gttgaaacaa
    22021 gctggattcg aacaattcgt actagttccc gaacaagctg tggcagatcc agatttcact
    22081 acggtcaaat cgccaaatcc agaagaacat tcagcttttg aatatgctat acgacttgga
    22141 gaaaaagaag gagctgactt gctgattgca acagatcctg atgcagaccg tttaggcgca
    22201 gcggttcgtt tgccagatgg ttcttatgaa gtattgactg ggaaccaaat cggagcattg
    22261 tttgctcaat atattttgga agcacatcaa tcaagaggca ctttaccaga aaacgcagcg
    22321 gtattgaaat caatcgtttc cagcgaattg cctacagcaa ttgcggaaca ctacggtgct
    22381 accatgttca atgtgttgac agggtttaaa ttcattgctg aaaaaatcca acaatttgaa
    22441 gaagaccatt cacatacttt tatgtttgga ttcgaagaaa gttatggtta tttagttaaa
    22501 ccttttgttc gcgataaaga cgcgatccaa gcattgcttt tattagcaga agtagcagct
    22561 tactataaaa aacaaggcaa aacgttgtat gacggcttgc aaacgatttt tgaaacctat
    22621 ggctatttcg cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa
    22681 atctctgctt tgatggaaaa attccgtaaa gaagcaccga cagaatttgc cggagcaaaa
    22741 gttattcaaa cagaagactt taaagaattg acgagaacag accgtgaagg aaacgtagaa
    22801 aaactaacaa caccgccatc cgacgtattg aagtatacat tggaagaaaa tggctggatc
    22861 gctatccgcc cttcaggaac tgagccgaaa atcaagtttt atattggtgt aaaaggaagc
    22921 tctacagaag acgcagccga aaaagtagcc tcttatgaag cgattatcaa aaagatgaca
    22981 gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt
    23041 tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc aaaagtagag aaagtcagtc
    23101 aattattcaa aatgttgagt gatcccactc gtcttaaaat tctgctatat ctgaaagacg
    23161 gggaacaaaa tgtgacagca atcacccaag cagtggaaat ggagcagtct gctgtatcgc
    23221 atcaactacg tttattaaga gaaaatcatg tcgtcaagtc tcatcgcgag ggaaaagcga
    23281 ttttgtatag tcttgatgat tctcatgtac tggatatttt gaaccagaca ctcaaacatg
    23341 tggaacatca ataacgatag gtgaaaatag ataaacactg gagtgtgaca acgaagaaat
    23401 ttcgttgtca cactccagtg ttttttaaga aaaagacaaa aatgattaat cgcttttttc
    23461 tgttgtttca gttccataat aactatgatc ttctaggtct aaagcattca aaataagcga
    23521 agccgtttct tcaatagata aagatgccac attgatcact acacatccta gtttttcata
    23581 aagatcattc gcaaaatcga gttcagctct aattttttcg atatcggaat aagcagtatc
    23641 agggtttaat ccgtaagagc gcatccgttc tttacggata ttatttaaaa tatctgggtt
    23701 attcgttagt cccacgattt ttttaggatc gatttcccac agctgtttgg gaatatgtgc
    23761 ttggggaatc aaaggtaaat tagcaacttt taaattttta tttgctaaaa acaagctcaa
    23821 aggcgttttg gaagttcggg atacgcctag caaaacgaca tctgcttcta aaaaaccacg
    23881 aggatctttc ccatcatcgt attttacagc aaattccatt gctttgatcc gtttgaaata
    23941 gttctcattc aataaatgga gggcaccagg ttgccgaatt ggcttgatac cagttaaacg
    24001 ttcaatttct gatatcggtg gcgtcatcac atcgaaacta aataattgat gttcttcaca
    24061 aaaagaacgt gttatctcga tcagttcttg attgattaat gtatgcagga caagtgcttt
    24121 ttcttctaat gcatcttgca atgcctgcct taatgttttt tcatctgtca cgaacgttct
    24181 gcggaatagg ttgaactcaa cggtaggata ctgagccata gtagcttgcg ctagttttgt
    24241 cgcagtttct ccagcagagt cggagatgac aaaaaaagta acgatttctg tagaatcgga
    24301 agacatggat aaaacctcac ttgcattttt tttatgttta ttataccata ctctagaatg
    24361 cgtacttcgt aatcattttg atttggagga ataagtcatg aaaaaacaga cattgatcga
    24421 acaatcatta gaaacattga aaaataacgg ttttaaatat actaaaaaaa gagaagcatt
    24481 gttaacttat ttagtgaagc gtaaccgcta cgtatctgct aaagaagttt ttgactatat
    24541 gaatcaggaa ttcaaagggg taagttatga tacgatttac cggaatctcc atgattttgc
    24601 agagatcgat ttactggaag aaaccgattt aaatggtgaa atgaagtttc gttttcattg
    24661 ctgtcagggg gaaatcggcc atcaccatca ttttatctgt acagtgtgcg gaacaacgaa
    24721 agaaatccat atgtgtccaa tgaattattt cgaagaacag ctaggtggat gcacgatcga
    24781 agggcataga ttcgagattt tcggaagatg tgaaaattgt caataaggat ttaaataaaa
    24841 taacatttga taatttaaaa aaaaagcaag atttccttga cggtcaaaac agcattagat
    24901 ataatattta caggacagtc ttgttttttg agatatattt caataacaat tacggttact
    24961 ttttaagctc ggagggaggg aattaacatg tcaaaaacag tcgttcgcaa aaacgaatca
    25021 cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa aagcgggtac tttacaagaa
    25081 tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac gcaagaaaaa atctgaagca
    25141 gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg agttggtagt gtgtcactac
    25201 taagtagatt aaacgatgac atgaaagcag cgatgaaagc gaaagataag gaatctttac
    25261 aagtcatccg aatgatcaag tcatctatac aaaacgagca gattaaagaa ggtcacgact
    25321 tgacagaaga agaagaacta acggtgttgt ctcgcgaaat gaaacagcga cgtgattctt
    25381 tgcatgagtt tgaagaagca ggacgtgacg atttagctga aaaagtaaaa agtgaaattg
    25441 taatagtcga aaaatatatg ccggaacaac tttccgacga agaaattcga caacttgttc
    25501 aagaagcaat cactcaaact ggtgcttcat caatgaaaga atttggcaaa gttatgggtg
    25561 caattatgcc taaagtcaaa ggaaaagcag atggaaacca agtgaatgcg attgtgaaag
    25621 aactattaca atcttaattt tatcatttaa actgcggaca gtgattgtcc gcagtttttt
    25681 gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag aataagattc tttcttctag
    25741 ctgttttttt cctgtttaat atctcttatg ttaaattgtg tttgatttgg ttattcttct
    25801 ggtagttcat ttgcttcttg tttataccct gataggcgat aggcgtaata aaagagtaag
    25861 ctgccagcgt aaccaagtaa cgttgggacc atccagccaa gtcctaatgc agcaaaagga
    25921 acgtgatgat gataaaatcc tagtaagctt gctacgacag tttgctgact cactaatgca
    25981 ggcgaatttg ccaacatatc caatacggct ggaacggcag aaaagaacat cgttacttga
    26041 tagacaaatg gactcttttg gaagaatttt gctgttaagg aaagcaagat taacgcaagt
    26101 gacaatggat acaaaagcat caatactggt aaagaccatt ggatgatatt atccaatccg
    26161 gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca gccatgtcat ataactgact
    26221 ttagggaaca gtttatggaa atcttgtgca aaagaaacaa cgagtcccat agcggtagtg
    26281 aagacaccta atgtgaccat tacccctaag aagctagcac cgaagttacc taaataaaaa
    26341 ttagtgatct gagaaagtgc agttccgcca ttttctgata aaggcatctt attcaaactc
    26401 atcgcaccga gaaggaccag accaaaataa atcatgactt ctaacgcaac gctcaggcta
    26461 ccagcttttg ctgttaattc tgtgactttt ttatctttaa aaccaagatc acgaactgca
    26521 tggacaaaag tgacacttaa agcaagtaaa gctactgcat caaccgtgtt gtatccttct
    26581 aaaacgccat ttgtcaaagc gttcgtctga taagaatctg cagcagattg gtttagattc
    26641 cccatgggtt ggaataaaga aacgacaaaa atgatagcta ataatgcaag aaacaaggag
    26701 tttaaatact ttccgacata tttgaccaaa cgtgttggat gtacagtaag gaaataagca
    26761 agtaaaaaga aaagagcaga gaataaaaac atacctaatg aatcatattt ttcaggcaaa
    26821 aagggtttag cagccatttc aaaagcagtt gctgctgtcc gcggtgtacc aaagaatggt
    26881 ccgatcgtta aatgaacaag gatcaagaac aaggcagcat accattttcc gacaggttta
    26941 gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc taataatggg
    27001 aaaagagcgc ctgagatggc aaatcctaca ccagcgctga tccagtttga tccagccatt
    27061 tgacctaagt ggacaggaaa gatcagatta cctgacccga agaacatccc gaagataagt
    27121 gaactgacca tcaataattg cttccatgaa taagtttttg tttcttccat aaatttttcc
    27181 tccaataatt atcattttga gtaacaaaat aaacgttgca gaaactagaa atgccactaa
    27241 tatcagattc catgaaaacc accaccttaa aatataaaaa aaagcccttt tccacttggg
    27301 aaaagggctc tagattctag aacggtacca ccttttttcg cttaaaaaag cttcttcatt
    27361 cacggacgct tttcgataaa gttatccgct agcctgataa tggaggcagc cacaatgatc
    27421 tacgttttcg acattgactc ggaaatgatg ttcgtctatt cgatttattg tcatttctca
    27481 cttacatgac tcactgggaa taaatcgctg aatagcctac tgttttcctc gacgctttgc
    27541 tgtatattta tttgttgacg ttataataca cctgtttttt ttgaaatgca agaatatttc
    27601 aaatattttg ttttcttttt ttgaaacaag aaaaataccc agagaatact tgctaagtta
    27661 gacggaatac ttgtcctcta tcttatgcat agcttacatg atcagctttt tttattcaaa
    27721 cgagaccttt agagaatgga agcagctatt ttttaacgtt tcttggtact tatggttaag
    27781 caactctttt agaacctttt cttatttgct atatagtttc ttctgagtat ggtaaaatag
    27841 gaaagaatga atcgaaaaaa aggggatgtc tatttttgac ggaagagtct agttctttag
    27901 agattagatt gacggctgct gatgatgtca gcatgctttt aggtgcacac gacaagcata
    27961 taaaactgat tgaagaaaca acagaaacaa ccatccatac acgtggagaa atcattcaga
    28021 tcattggcga acaatcccag atttccttgg ctgcctcagt cattcggaca ttgcaggaat
    28081 tgatcaaacg aggaatccat gtaagtacac cagatgtcgt gacggcactg aaaatggggc
    28141 aaaaaggcac attagattat tttgttgaaa tgtatgagga agagatcgtc aaagaccgta
    28201 atggaaaacc aatacgggta aaaaacagcg gccaaaagaa atatgtcgaa tcggtagcca
    28261 aacatgatat cgtatttggc gtaggaccag ccgggacagg aaaaactttt cttgcagtag
    28321 tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa aatcattttg acgcggcctg
    28381 ctgtggaagc aggggaaaat ctaggctttc ttcccgggga tctaaaagaa aaagtagatc
    28441 cttatctgcg tcctgtttat gatgctttat accaaatttt tggattagat catacgaacc
    28501 gattgatgga acgaggcgtg atcgaaattg ctcctttagc ttacatgcgg gggcggacat
    28561 tggatgatgc attcgttatt ttggatgaag cacagaacac gacagttgct cagatgaaaa
    28621 tgttcctgac acgtcttggt tatcaatcaa aaatgattgt caatggagat acgagtcaga
    28681 ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc agaacgaaca ttaaagcaaa
    28741 tcaagaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac cctgtagttg
    28801 ccgaaatcat caaagcttat gaaaaggcag atctccatca agaataagct ttgcttagga
    28861 ggataagcac atgttgaata aaacactgca gaaactacaa gaaaaacttg gaaaggcagc
    28921 attgcctttc ctgctgttct tgttttttat cattatctgc ggcgttacat ttagtagtgt
    28981 caaacaaaag gccaatgatt tcagagaagg ccaagtagca gaagaaagta tccgtgcgaa
    29041 caaaacgatc gagaacacgg aagaaaccga acaaaaaaga aaactggcag cagaagcagt
    29101 cactccagaa tacacttacc aaaaagatct tgcagatgac cagaacaatc ggatcaagca
    29161 actatttgaa ttgatcgata aaacgaatga tgccatcaac aaatcttatg acgaaaagat
    29221 tgataaggca aaggaagatg aaaatatccc aaagcctact acagaagagc ggatcgccgc
    29281 gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt gcgttttatc aaaaactacc
    29341 gaacaatttt tattcgacga tatttgaaat gactgaaact gagatccata ctgtgaggga
    29401 tgagagtctg aagctcattg acgaacagat gagcaaacaa gttcgtgaaa gtgaattgga
    29461 agcttttaaa caagaagcag aagagcaaat ccaatatttg aatgtcacac cagcacaaca
    29521 acaaatgatt cgctacttag tggatcaagg tatcgtagtg aatgacgtat tgaatgagaa
    29581 aaagacagaa gaattgaaac aatccgcacg agaagctgta caaccagtga tgatcttcca
    29641 aggagaaatc attgttcgtg aaggtaatca aatcgatgct tcagctatga aaaaattgga
    29701 attactagga ctgactaatc aaacaacttc gatttttccg ctaattgcca tggttttagc
    29761 tgttttgctt caaatcggcg tactgattta taattcactt caattccaaa atgcaggaga
    29821 aagaacaaaa tacgtgcttt tctatgtgac agctatgtca gtcagcatag tgctgatgaa
    29881 atttttccaa ttattccaaa cggaacaagc tacttatatc ccgctgtttt atcccgctgc
    29941 ttttgcacca cttgttttaa gctttttctt gaaccgaaga gcagggatca tggctggtat
    30001 tttccaagca gtatctgcac ttttcatttt ttatgattcg attggcacga actttctaac
    30061 gattatcttg atgtcttatg ttttctctgg gctcatggca acgattgtca gacgcaaacg
    30121 gatctcagaa caaggattat ctgctgccat gtgggtcatt atttttcctg tgatgatgga
    30181 tgtcattttg atcgtctatc aaggaatgag ctttggtgat gcaacgactt ggttaatgct
    30241 tgtctgcggg ttcactggtg cactcttctc attcttgctg acgatcggtc tacacccata
    30301 tattgaattg atggtcaatg atgacagtgt aattgtatta aatgaactca gcaatccaaa
    30361 tcatccttta ttgaaacagt tgctggaaga agcgccaggt acttatcatc atagtatgat
    30421 ggtagctaat ctaagtgcta acgctgtagc agaaatcggc ggacgttctt tgctaactcg
    30481 agtagcttgc tattatcatg atatcggcaa gatcaaacat gctagctttt tcgttgagaa
    30541 cttgccttca ggagcggaaa acccgcacaa tttcttattg ccagaagaca gtaaacatat
    30601 tatctttgga catgtgacag atggagccaa aatattggaa gattataata tgccgcaaat
    30661 ggtgatcgat atttgcaaac agcatcatgg aaccacgcta atgcgttatt tctacatcaa
    30721 agcgaaagaa cgcaatccgg aagtaacaga agagcaattc cgttatcctg gaccaaaacc
    30781 gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg cagtacgggc
    30841 aatggatcat ccgacgagcg aaaaaatcga aagttttgtc cataatctta tcgaagagcg
    30901 tatttccgat ggtcaattag acgatagtgg actgaccttg aaagaaatcc gaaaagtgga
    30961 aaaatcattg atcagcggtt taagtagcac cttccattct cggatcaaat atcctaaaat
    31021 gaaaacagaa gttgagaaga taaaggaaga acaagaagaa tagaaagaag cgatgtataa
    31081 tggatattac ctttatggat gaaacaaacc aagtctcaga agagaaaatg aaagaaatcg
    31141 atgacttgct gcaatttgca gcatcttatt taaagctgcc agaggaaaca gaaatgtctg
    31201 ttactttcat ggacaatgcg gcaatccaag taatcaatcg tgattaccgt ggaaaagatg
    31261 caccgacaga cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa atcccagtga
    31321 tttttgaaga tggagagaat ccattgcctc gtgtactagg agacatcatg atttctattg
    31381 aacgtgctgc tgaacaagca gaagcttacg gccattcata tgatcgggaa cttggctttt
    31441 tagcggtaca tggattcttg catatcaacg ggtatgacca tatgacacca gaagatgaga
    31501 aagaaatgtt tggtttacag aaagagatct tggatgccta tggactcaaa agataaaaac
    31561 gtagagaaaa acaaacattt cctgatgtct gtggaatttg ctgtccaagg agtcaaaacc
    31621 gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct tgctttattt
    31681 atgggcgtga ttttccagct tgatcggata gagtggttat ggctgctttt agctgttttt
    31741 ctcgtttgga tagtagagat catcaatact gtttttgaaa atgtagtgga catgtttacg
    31801 gattttcatt ttcatccgat tgggaaaaaa atcaaagata tggcagctgg agcagtttta
    31861 gtgaccgctt gttttgctgt catcattggg ttgattttat ttgtccctaa aatatatcat
    31921 ttgttgttta attaaaaagg agaacgtata tgccagaaca taaatcaggg tttgtcgcaa
    31981 tcgtcggccg cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg ggtcaaaaaa
    32041 ttgccattat gagtgacaag gcacagacaa ctagaaataa aatccaagga gtatatacta
    32101 caccagaagc tcagctgatt tttatcgata caccaggaat ccataaacca aagcatcgtc
    32161 taggagattt tatggtagaa gcagcttaca gtgcgttgaa agaagtggat gcagtcttat
    32221 ttatgatcag tgctgatcaa aaacgcggcc ggggagacga ttttattatt gaacgtctaa
    32281 aaaacgtgca ttctccagtt tatctgatta tcaataaaat cgataagatc catccagacg
    32341 agttgctggg aatcatcgaa gattattcga cgcagatgcc ttttgcacaa gtgattccga
    32401 tctctgctac agaaggaaac aatgtcgaac gcttaatgga tgtattagtc agtgaaatgc
    32461 cagagggccc acaatatttt ccagatgatc aagtgactga tcatcctgaa tactttatcg
    32521 tttctgaatt gatccgtgag aaagtcttgt ttttaacacg agacgaagtg cctcattctg
    32581 tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga taaaatccat atccaggcca
    32641 cgatcatcgt cgaaagagat agccaaaaag gaatcattat cggcaaaggc ggaaaaatgc
    32701 tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga gacaaagtat
    32761 ttctagaact ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc tatctgcaag
    32821 actttggcta ccgaaaagac gaatactgat attctgaact tcttataggt taagtataga
    32881 aataaactgg ataaaaatcc gcacgttgag aaaaacggcg gaagatcgaa acataaataa
    32941 tctggatcgg cagaatctgc ttttccagat ttatttttta ggagggagaa tatgagccag
    33001 cttgcagaat cgaaaggctt gattttatct agccgtaatt acaaagaaaa agataaatta
    33061 gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt atatcaaagg aatccatcgg
    33121 caaaatcagc cattagctcc ggctttgctc ccctttaccg aagcagtcta tatcggtaat
    33181 tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc gtttcggaac
    33241 atccaagaag atatcttttt gagcggttat ggtacctatt tgctaaatct agtagatgca
    33301 gcaatcgaag atcatcaata tgacccgcat ttattccaat ttacgcaaca agctctgcag
    33361 cggatggatc aaggagatga tgcagaaatc atcacgaata tctttgaagt tcaaatactg
    33421 caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga aacaaaagga
    33481 aaatttgact attcttccaa atctggcggt gtgatatgcg agaaacactg gccaatggac
    33541 gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt ttcggctatt
    33601 tcttatgata agatcagtgg aatccacttg aaagaagaaa cgaagcaggc aatccgtgac
    33661 ctcattgacc agttgtatga tgaatatgtc ggtattcatc taaaaagcaa aaagttcatt
    33721 gatcaaatga agtcttgggc acacgtatta aagacgccag agaaaaatat gcctgaaaaa
    33781 gagatacctg aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa actatttgac
    33841 aaatacaggg acttccgtct attattaacc gtagattaaa tagatcgttc actgagagga
    33901 tctattatat aaacaagaaa actgcaaaat gaatacgtac attgaccaag atcagtaaaa
    33961 aacacgtggt ttagcgagtt cgggagagtg gaagccggac ccaaacgatt tttgaagagg
    34021 cacttgcgag tacgataaat gaagctgccg aatctttatt cggaagtagg gtggaaccgc
    34081 gaaattattc gtccctatgt ctgtaatcag acatagggac tttttgtcgt tttctttcct
    34141 gtattcttat tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg agtaaaaaat
    34201 tgaccgtcca agatatgatt ttaacattac aaaaattttg gtcatcaaac ggctgcatgc
    34261 tgatgcaagc ttatgacaca gaaaaaggag caggtacgat gagcccatat acattcttac
    34321 gggcaatcgg accggaacca tggaatgctg cttatgtaga gccatcccgc cgaccagctg
    34381 acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca tcatcaattt caagtcgtaa
    34441 tgaaaccttc acctgaaaat atccaagagc tttatttaga aagcttgcgt cttttaggaa
    34501 ttgatccatt agaacacgat attcgttttg ttgaagacaa ctgggagaac ccatcgatgg
    34561 ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat ggagatcaca caattcacgt
    34621 acttccagca agtcggagga ctaccttgta aaccagttac ttctgagatc acttatggac
    34681 tagaacgtct tgcttcatat attcaagaag ttgaaagtgt ctatgatctg gaatgggctg
    34741 atggtgtgaa atatggcgag atcttcaaac aaccagaata tgaacattcc aaatattctt
    34801 ttgaagtaag tgaccaagac ttgctactaa gcaactttga ccgttttgaa aaagaagcta
    34861 aacgctgtat cgatgaaaat cttgttcatc cagcgtatga ttatattttg aaatgcagcc
    34921 atacattcaa tttattagat gctcgcggtg cagtatccgt aacagaacga gcaggttatc
    34981 tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca gaacgcgaaa
    35041 aattaggatt ccctttattg aataaaaaca cagcaaatcc agaaaacatg aaggaggctg
    35101 aataagatgg caaaagactt attacttgaa atcgggctgg aagaaatgcc tgctcatgtt
    35161 gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa aatttttaga tgaaaaccaa
    35221 ttgacttatg atacgatcga aacgttttct acaccgcgcc gcttagcaat caaagtaaca
    35281 aacattccag aacgtcaagc agacagtgaa gaagaagtca aaggaccagc caaaaaaatt
    35341 gctttggacg cagatggaaa ctggacgaaa gcagctgaag gatttgtccg cggacaaggt
    35401 ctgacgacag atgctatcac ttttagagaa ttaaaaggcg tggaatatgt ctatgtaacg
    35461 aaatacactc acggcaaaga agcgaaagaa gtattgacag gtttagcaga tgtcattaaa
    35521 agcctcactt tcccagtaac gatgcattgg gcagaatacg attttgaata tatccgccct
    35581 attcactgga tcgtggcatt gttagatgac gaagtaatcc cgtttgagat cttagatgtc
    35641 aaaacaggca gagcctctcg aggacatcgt ttcttaggcg aagatgtgac atttgcgcat
    35701 gcaaaagaat atgaagaaaa attgaatgaa caattcgtta ttgcttcacc gcaaaaaaga
    35761 caagaaatga tcgttacaca agcaaatgaa ttagccgaaa aaaatcactg ggttattgat
    35821 ctagacgaag atttactaga agaagtcaac aatttagtcg aatatccaac tgtcttttct
    35881 ggcgacttcg aagaaaaata tctttctgtt cctgaagaag tactagtcac ttctatgaaa
    35941 gaacatcaac gttatttcga ggtacggaac gatcaaggta tgctgcttcc tcattttatt
    36001 tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg ttcgaggcaa tcaaaaagta
    36061 ttgacggctc gtttagaaga tgcggaattc ttttataatg aagacaagaa agcttcaatc
    36121 gaccaatacg ttgaaaaact aaaaactgtt actttccatg aaaaaatcgg tagtatctat
    36181 gagaaaatgc agcgggtaca agtcattgcc cgtatcattg gcgaacaagt aggactatct
    36241 gaggaagaat tgatggatct gcaacgagct gctgaaatct ataaatttga tctagtaacc
    36301 aacatggtag gggaatttcc tgaactacaa gggatcatgg gcgaaaaata tgctctgctg
    36361 aaaggcgaga aacctgctgt agcaacagcg attcgtgagc attacatgcc tatatccagt
    36421 gaaggagagc tacctcagtc tacagtaggt tctgttttag cgattgcaga taaattagat
    36481 agcatttttt ctttctttgc agttgggatg attccaagcg ggtcaaacga tccttatgcc
    36541 ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat cgaaaaaatg ggcctttcct
    36601 ttgtctgttc tgcaagaaac gattagtgat gttatcagta aagatacgga tcgtttcggc
    36661 attggtttat cagaagggca acaacaggtg atcgacttta tcaaaggccg tctgcgccaa
    36721 ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag ctgttttgaa tgcagaacaa
    36781 aaagatctga caaaagtatt tgcagcagca caattgttca agcagcatct tgcagatgaa
    36841 gacttcaaac catcgatgga agctttgaca cgtgtcgtga acttagcgaa aaaagcggag
    36901 cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa atgaagcgga gaaagaactg
    36961 cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc aaacgatcaa cgaaaattat
    37021 gatcagcttg taagccttcg cccactgatc gaaaattatt ttgatcagac gatggtcatg
    37081 gcagaagaac cagcagtacg taagaaccga ttaacgcaat taagtcaaat tgctgccatg
    37141 gctttgtcgc tagcaagttt agatcaaatc atcacaaaat aatttctaat atgtaaataa
    37201 accagagaat aggcacaaaa
//
LOCUS       DARDHU010000017        32148 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000017 DARDHU010000000
VERSION     DARDHU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.017"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(407..766)
                     /locus_tag="SDD03_000908"
     CDS             complement(407..766)
                     /locus_tag="SDD03_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG4303374.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="SDD03_000909"
     CDS             complement(796..1137)
                     /locus_tag="SDD03_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303375.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="SDD03_000910"
     CDS             complement(1134..1808)
                     /locus_tag="SDD03_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4303376.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SDD03_000911"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SDD03_000911"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG4303377.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="SDD03_000912"
     CDS             complement(4322..5512)
                     /locus_tag="SDD03_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4303378.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="SDD03_000913"
     CDS             complement(5533..6060)
                     /locus_tag="SDD03_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG4303379.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="SDD03_000914"
     CDS             complement(6107..8896)
                     /locus_tag="SDD03_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4303380.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="SDD03_000915"
     CDS             complement(9043..9243)
                     /locus_tag="SDD03_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4303381.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="SDD03_000916"
     CDS             complement(9696..10016)
                     /locus_tag="SDD03_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303382.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10061..10204)
                     /locus_tag="SDD03_000917"
     CDS             complement(10061..10204)
                     /locus_tag="SDD03_000917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303383.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            complement(10519..11637)
                     /locus_tag="SDD03_000918"
     CDS             complement(10519..11637)
                     /locus_tag="SDD03_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303384.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="SDD03_000919"
     CDS             12108..13415
                     /locus_tag="SDD03_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG4303385.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="SDD03_000920"
     CDS             13536..14735
                     /locus_tag="SDD03_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG4303386.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="SDD03_000921"
     CDS             complement(14762..16030)
                     /locus_tag="SDD03_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG4303387.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="SDD03_000922"
     CDS             complement(16200..16841)
                     /locus_tag="SDD03_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG4303388.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="SDD03_000923"
     CDS             complement(17250..17753)
                     /locus_tag="SDD03_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4303389.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="SDD03_000924"
     CDS             complement(17876..18640)
                     /locus_tag="SDD03_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG4303390.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="SDD03_000925"
     CDS             18748..19023
                     /locus_tag="SDD03_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG4303391.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="SDD03_000926"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="SDD03_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG4303392.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="SDD03_000927"
     CDS             complement(20579..20980)
                     /locus_tag="SDD03_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4303393.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="SDD03_000928"
     CDS             complement(20994..21803)
                     /locus_tag="SDD03_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4303394.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="SDD03_000929"
     CDS             complement(21790..22680)
                     /locus_tag="SDD03_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4303395.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="SDD03_000930"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="SDD03_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="SDD03_000931"
     CDS             complement(23348..24289)
                     /locus_tag="SDD03_000931"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG4303396.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SDD03_000932"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SDD03_000932"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG4303397.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="SDD03_000933"
     CDS             complement(25274..26470)
                     /locus_tag="SDD03_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4303398.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="SDD03_000934"
     CDS             complement(26488..27216)
                     /locus_tag="SDD03_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4303399.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SDD03_000935"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SDD03_000935"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG4303400.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="SDD03_000936"
     CDS             complement(28628..29086)
                     /locus_tag="SDD03_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4303401.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="SDD03_000937"
     CDS             complement(29083..30546)
                     /locus_tag="SDD03_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4303402.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SDD03_000938"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SDD03_000938"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG4303403.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32148
                     /locus_tag="SDD03_000939"
     CDS             31602..>32148
                     /locus_tag="SDD03_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4303404.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNK"
BASE COUNT         9378 a         6919 c         5510 g        10341 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataaga
//
LOCUS       DARDHU010000018        31840 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000018 DARDHU010000000
VERSION     DARDHU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.018"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(151..2304)
                     /locus_tag="SDD03_000940"
     CDS             complement(151..2304)
                     /locus_tag="SDD03_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG4303405.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="SDD03_000941"
     CDS             complement(2598..3446)
                     /locus_tag="SDD03_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4303406.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="SDD03_000942"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="SDD03_000942"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG4303407.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="SDD03_000943"
     CDS             complement(5947..6126)
                     /locus_tag="SDD03_000943"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG4303408.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="SDD03_000944"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="SDD03_000944"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG4303409.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="SDD03_000945"
     CDS             8274..10553
                     /locus_tag="SDD03_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4303410.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="SDD03_000946"
     CDS             complement(10646..11293)
                     /locus_tag="SDD03_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303411.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="SDD03_000947"
     CDS             complement(11472..12191)
                     /locus_tag="SDD03_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4303412.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="SDD03_000948"
     CDS             complement(12322..13473)
                     /locus_tag="SDD03_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG4303413.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="SDD03_000949"
     CDS             complement(13690..14430)
                     /locus_tag="SDD03_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4303414.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="SDD03_000950"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="SDD03_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG4303415.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="SDD03_000951"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="SDD03_000951"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG4303416.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="SDD03_000952"
     CDS             complement(15391..16041)
                     /locus_tag="SDD03_000952"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG4303417.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="SDD03_000953"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="SDD03_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG4303418.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="SDD03_000954"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="SDD03_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG4303419.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="SDD03_000955"
     CDS             complement(17484..18011)
                     /locus_tag="SDD03_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG4303420.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="SDD03_000956"
     CDS             complement(18191..18976)
                     /locus_tag="SDD03_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG4303421.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="SDD03_000957"
     CDS             complement(18969..19838)
                     /locus_tag="SDD03_000957"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG4303422.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="SDD03_000958"
     CDS             complement(19839..21218)
                     /locus_tag="SDD03_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG4303423.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="SDD03_000959"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="SDD03_000959"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG4303424.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="SDD03_000960"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="SDD03_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG4303425.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="SDD03_000961"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="SDD03_000961"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG4303426.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="SDD03_000962"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="SDD03_000962"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG4303427.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="SDD03_000963"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="SDD03_000963"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG4303428.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="SDD03_000964"
     CDS             complement(25176..25400)
                     /locus_tag="SDD03_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG4303429.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="SDD03_000965"
     CDS             complement(25453..26415)
                     /locus_tag="SDD03_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG4303430.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="SDD03_000966"
     CDS             complement(26425..26865)
                     /locus_tag="SDD03_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4303431.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="SDD03_000967"
     CDS             27171..28136
                     /locus_tag="SDD03_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG4303432.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="SDD03_000968"
     CDS             complement(28193..28423)
                     /locus_tag="SDD03_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG4303433.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="SDD03_000969"
     CDS             complement(28509..29396)
                     /locus_tag="SDD03_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG4303434.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="SDD03_000970"
     CDS             complement(29485..30708)
                     /locus_tag="SDD03_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4303435.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31771)
                     /locus_tag="SDD03_000971"
     CDS             complement(30725..31771)
                     /locus_tag="SDD03_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4303436.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8794 a         6970 c         5488 g        10588 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt
//
LOCUS       DARDHU010000019        31648 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000019 DARDHU010000000
VERSION     DARDHU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.019"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(234..2126)
                     /locus_tag="SDD03_000972"
     CDS             complement(234..2126)
                     /locus_tag="SDD03_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303437.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2123..3850)
                     /locus_tag="SDD03_000973"
     CDS             complement(2123..3850)
                     /locus_tag="SDD03_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303438.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3866..4516)
                     /locus_tag="SDD03_000974"
     CDS             complement(3866..4516)
                     /locus_tag="SDD03_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG4303439.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4720..5016
                     /locus_tag="SDD03_000975"
     CDS             4720..5016
                     /locus_tag="SDD03_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4303440.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5188..5808
                     /locus_tag="SDD03_000976"
     CDS             5188..5808
                     /locus_tag="SDD03_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG4303441.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5900..7066)
                     /locus_tag="SDD03_000977"
     CDS             complement(5900..7066)
                     /locus_tag="SDD03_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4303442.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7078..7431)
                     /locus_tag="SDD03_000978"
     CDS             complement(7078..7431)
                     /locus_tag="SDD03_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG4303443.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7559..8581
                     /locus_tag="SDD03_000979"
     CDS             7559..8581
                     /locus_tag="SDD03_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG4303444.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8600..8761
                     /locus_tag="SDD03_000980"
     CDS             8600..8761
                     /locus_tag="SDD03_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303445.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9020..9454)
                     /locus_tag="SDD03_000981"
     CDS             complement(9020..9454)
                     /locus_tag="SDD03_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303446.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9564..11423)
                     /locus_tag="SDD03_000982"
     CDS             complement(9564..11423)
                     /locus_tag="SDD03_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4303447.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11541..12701)
                     /locus_tag="SDD03_000983"
     CDS             complement(11541..12701)
                     /locus_tag="SDD03_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG4303448.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13008..14432)
                     /gene="araA"
                     /locus_tag="SDD03_000984"
     CDS             complement(13008..14432)
                     /gene="araA"
                     /locus_tag="SDD03_000984"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG4303449.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14448..15146)
                     /locus_tag="SDD03_000985"
     CDS             complement(14448..15146)
                     /locus_tag="SDD03_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG4303450.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15103..16764)
                     /locus_tag="SDD03_000986"
     CDS             complement(15103..16764)
                     /locus_tag="SDD03_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG4303451.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16982..18382
                     /locus_tag="SDD03_000987"
     CDS             16982..18382
                     /locus_tag="SDD03_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG4303452.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18634..19713)
                     /locus_tag="SDD03_000988"
     CDS             complement(18634..19713)
                     /locus_tag="SDD03_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4303453.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19799..20758)
                     /locus_tag="SDD03_000989"
     CDS             complement(19799..20758)
                     /locus_tag="SDD03_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG4303454.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20770..22281)
                     /locus_tag="SDD03_000990"
     CDS             complement(20770..22281)
                     /locus_tag="SDD03_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG4303455.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22303..22866)
                     /locus_tag="SDD03_000991"
     CDS             complement(22303..22866)
                     /locus_tag="SDD03_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG4303456.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22872..23735)
                     /locus_tag="SDD03_000992"
     CDS             complement(22872..23735)
                     /locus_tag="SDD03_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4303457.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23749..24645)
                     /locus_tag="SDD03_000993"
     CDS             complement(23749..24645)
                     /locus_tag="SDD03_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4303458.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24820..26109)
                     /locus_tag="SDD03_000994"
     CDS             complement(24820..26109)
                     /locus_tag="SDD03_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4303459.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26081..27598)
                     /locus_tag="SDD03_000995"
     CDS             complement(26081..27598)
                     /locus_tag="SDD03_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG4303460.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27796..28686
                     /locus_tag="SDD03_000996"
     CDS             27796..28686
                     /locus_tag="SDD03_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG4303461.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28759..29583)
                     /gene="nadE"
                     /locus_tag="SDD03_000997"
     CDS             complement(28759..29583)
                     /gene="nadE"
                     /locus_tag="SDD03_000997"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG4303462.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29595..31067)
                     /locus_tag="SDD03_000998"
     CDS             complement(29595..31067)
                     /locus_tag="SDD03_000998"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG4303463.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9261 a         6642 c         5475 g        10270 t
ORIGIN      
        1 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtaaaat
       61 cagcggtttg acctgagtat tagagataca aaacggaaaa atagcttctc atattttgcc
      121 gttttttgag ctaataccga aggacagctg attgaacacc gtttattcgg aaaaagggac
      181 tgtgacacga cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa
      241 gcttcttctt cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc
      301 aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct
      361 gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg
      421 cggttcatcg ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca
      481 tcaaggatca ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc
      541 tgcccttgcg aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat
      601 ttacgaacaa aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt
      661 gcatcgtcgt tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct
      721 tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca
      781 ccatcgtaac gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt
      841 gtcgtcttcc ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc
      901 aagttaaaat cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa
      961 cgaactttgt atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct
     1021 tcttcatcca acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc
     1081 atcaaacttg caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct
     1141 tgaacatccc ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg
     1201 aacacatagc ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat
     1261 tgagctttcc aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct
     1321 ttttcagtat gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg
     1381 ttattcaata aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct
     1441 acgatcatca cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc
     1501 cagctgattg aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat
     1561 tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac
     1621 atgatatccc catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct
     1681 tccaaatcat gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc
     1741 tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc
     1801 gcgattttat caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga
     1861 gcgccagcca ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt
     1921 tggatttgga agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata
     1981 cgtttagaca tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca
     2041 ccttttacac cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga
     2101 gagggacgtg tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga
     2161 tttcttgata agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt
     2221 ttccttcatc tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa
     2281 ctaaaacggt gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg
     2341 ttttgaaatc caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta
     2401 atgcccgtgc aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt
     2461 ccacatggct atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt
     2521 ctaatgcttt ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc
     2581 ggatcgttcc agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat
     2641 tatattgagt catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt
     2701 catataatcg agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga
     2761 ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg
     2821 ctccatgata tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag
     2881 aaactgtttt aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa
     2941 tacggtcagc tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa
     3001 agctgatcaa gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag
     3061 tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga
     3121 tcaatgtcat cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg
     3181 tataatcttt attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc
     3241 gaatcactct gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct
     3301 tttgcatact tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca
     3361 ttactgggat gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta
     3421 tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa
     3481 ccatttggat ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt
     3541 tatcaaacgc atcttttgtc agactctctg attttttata gacatccgta cgcaattgct
     3601 tacccagttt ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac
     3661 tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt
     3721 gcgctacccc tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta
     3781 cttggatcac catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga
     3841 tttttagcat ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt
     3901 tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc
     3961 gatacgaatg cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca
     4021 attcatgatc attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg
     4081 catcttgttt gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag
     4141 cgacttttct tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat
     4201 tctcgccttc caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata
     4261 gatcaccatc ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg
     4321 tctcaaagta ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc
     4381 tagggatatc cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag
     4441 aaaactcgat tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa
     4501 atgtctgtgt cggcatatgg atcctccttt ctttcaagat gacacattgt catatgacgc
     4561 tgtgtcatta taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat
     4621 catccgatac attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc
     4681 ccattaaaat agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc
     4741 caagacttca ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc
     4801 ttagattaca caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca
     4861 agacaattaa aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg
     4921 cgatcagaag cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt
     4981 ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga
     5041 ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta
     5101 ttcttcaacc ttatcaatat gacttgatag ccttttcttt ttctcatagt tataacaatc
     5161 atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa aggtcatccc
     5221 ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac aagttataaa
     5281 gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga tattccggaa
     5341 atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga attcacttcc
     5401 ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga tcagttcctt
     5461 gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc aacaaaatta
     5521 aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac agaaaacggg
     5581 ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg cggtgtatac
     5641 aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt aggcatcgat
     5701 caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc agatgagttg
     5761 atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa attgagataa
     5821 atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtacc ttggatattt
     5881 tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct tcataaagag
     5941 aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct gctgcataaa
     6001 caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc agcagaacta
     6061 ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga gagatcaagc
     6121 gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc atgaatggac
     6181 ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct aagtaaggga
     6241 tcaggttcaa aaaacccgca ataatcccca acgtgacagc atactctaaa ccaatgaccg
     6301 caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt cctcttatat
     6361 aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt tgcatgcgtg
     6421 tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat agataaaata
     6481 agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg acaaaacttc
     6541 caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc gaattcatga
     6601 acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa
     6661 tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg atgaagctca
     6721 ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt agtgcaacaa
     6781 ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt tcaaagtaat
     6841 caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc agacctacga
     6901 ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga
     6961 tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt aaaaaccagc
     7021 gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt cctcctgtta
     7081 gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat gaagatagat
     7141 tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata cgtgagctac
     7201 tgtctctaaa ttcccattgg ccatcggacc gacataatcg atcgtatata cctgatcatc
     7261 aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata cttcttgaat
     7321 caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca gcatgggttc
     7381 ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca ttgcactctc
     7441 tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg tgtggaataa
     7501 ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat cagctcatat
     7561 gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat
     7621 cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg aagaaaatag
     7681 ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta gtgtggatgg
     7741 attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg taacagagtc
     7801 tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat acggggcgaa
     7861 ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac tgaaagcagc
     7921 agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc atgcgcatgt
     7981 ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg gtacagatcg
     8041 tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta ccttcactgc
     8101 agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat tcgcttattt
     8161 atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa caggtgcttt
     8221 tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa atggaggggc
     8281 agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag gacataactc
     8341 gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc aaatcatctc
     8401 aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt ttgtcgtgtg
     8461 tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa caggattact
     8521 ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta
     8581 gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac gggaagaact
     8641 aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag aggaatatca
     8701 gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg tagaaaacta
     8761 atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt ctatcctgat
     8821 gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa aggagtattt
     8881 actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt atcataaagg
     8941 attttgatca ctctatcata aaggtaatat tacatttata ttagataact ctagttttta
     9001 ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt tggatggcag
     9061 ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat agatcttcat
     9121 ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct acatatgaaa
     9181 gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac
     9241 tgactttctt aagcattgcc cgatattttt ttgttccttc ttcaaataac aggatgatct
     9301 cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg tttctgatca
     9361 cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg atttgatcga
     9421 tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt tctccattgt
     9481 tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa aaaatttaat
     9541 ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc taaactatat
     9601 tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat ctcttgttgc
     9661 gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag ttctcgttca
     9721 aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt tgttgtctga
     9781 ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc
     9841 gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc atgcaatgtt
     9901 tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg atccccccaa
     9961 aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg cttcagtgcc
    10021 gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc acttccatct
    10081 gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat atgtgtctgc
    10141 atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt cattacttct
    10201 ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt ataggtcatc
    10261 gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc tcttttatac
    10321 atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa
    10381 acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt ggtgagttgc
    10441 gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt tgctgttcca
    10501 ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac ttcataataa
    10561 tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc tgagaaactt
    10621 tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt tttttcatcg
    10681 gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata tccttcaggt
    10741 tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc agtgttatag
    10801 tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc ttttcgcaac
    10861 aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc cacaagcagt
    10921 gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa catgtaccag
    10981 caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag catagtgccg
    11041 gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg ataaatcggt
    11101 tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat ggctacgaca
    11161 tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat aagaataatc
    11221 agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac
    11281 gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact aaaaacaaca
    11341 agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa
    11401 agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata aaagaatgtt
    11461 gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa gaattaatga
    11521 aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg gacctttcgt
    11581 actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa atcagtactg
    11641 tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat atctgcgatc
    11701 gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt tttcttttga
    11761 taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg ataattgacc
    11821 gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc caaacgtccg
    11881 ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag tatacggtca
    11941 ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg ttgtttttcc
    12001 aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt gataagtaca
    12061 gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt atcttctgtc
    12121 aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa atctgcttgc
    12181 cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat ccctaagcta
    12241 tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc gatggttttg
    12301 ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc taaaatatta
    12361 ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga agataagagc
    12421 aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt attcatgacg
    12481 atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac accagctctt
    12541 gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact acgaataact
    12601 attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa cttttctatc
    12661 tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc tccttactaa
    12721 caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc ctagaagcaa
    12781 aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac aagttactgc
    12841 cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata aatggtgttc
    12901 cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca aatttcgtca
    12961 atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta tctaataacc
    13021 actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt tagatttagc
    13081 gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc ttcatggaaa
    13141 ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc agtcgttggt
    13201 tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc tgccatcgat
    13261 acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc ttctctatct
    13321 cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt cggatctact
    13381 tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta agtataatct
    13441 tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc aatcgctgca
    13501 gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg ttgtacagct
    13561 agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa agcgttatag
    13621 cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac ttttacatgt
    13681 tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc cttgtactct
    13741 tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac aagatctccg
    13801 ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt gtctccttct
    13861 gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc aaaactttca
    13921 ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc aggtcgtttc
    13981 cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa cccatattcg
    14041 cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt tttccaagga
    14101 atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag ctttgtcccg
    14161 cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc tgctacttct
    14221 tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac tgctaattct
    14281 tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac catttcttgt
    14341 gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga tcctacaaca
    14401 aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta tttgttttgt
    14461 ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat ccgttgaggc
    14521 agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca tacttcatct
    14581 agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa tggaccgtgt
    14641 ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt gaatgtttct
    14701 atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg tgtcaaaaat
    14761 cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat cgcaggaaca
    14821 tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac gattcctccg
    14881 atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga gggattgagt
    14941 ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc tgtcatctct
    15001 tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc aaaagcgctc
    15061 acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt tgcttggtat
    15121 acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca attgatgaat
    15181 cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct gatagcctgc
    15241 tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca cgtcttctgc
    15301 caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta ataacgcgat
    15361 tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct ccatagcaga
    15421 tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc ccactaaacg
    15481 atcaatttgg acattctcag atttcaatat atccattccg attctcattg caccaaaagc
    15541 actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg gtgtacgtac
    15601 aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat aaccataaga
    15661 aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata ccgtgaataa
    15721 tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa tactgaccca
    15781 agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt ctcctgaagg
    15841 tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta atacgatcat
    15901 agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccgt ttggctacac tgttcgtagc
    15961 gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg gtctcaaatt
    16021 tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt cttctcctgc
    16081 tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt tttcgattaa
    16141 ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat cgataggaaa
    16201 catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc aatggacata
    16261 acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat tcaaaataga
    16321 ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt tttccgttaa
    16381 tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta ctagtaactg
    16441 ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa tcgacccgat
    16501 tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc ggtaactgac
    16561 gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt ctagtttgtt
    16621 ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca atacagcttt
    16681 gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga catctaacat
    16741 actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc aaacctgatc
    16801 aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg tacatgtcaa
    16861 gcggttattt acaagaatac aataaaaaac acttgttttg tacgtacata agttataaaa
    16921 tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg agagaatact
    16981 tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg catttggcgg
    17041 cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt tacagcatga
    17101 ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg tcatgttagg
    17161 tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac gacgaaaaat
    17221 gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat ctgcccctca
    17281 caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg ttggcgctgc
    17341 atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa ggggaagatt
    17401 gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg ttgcagatta
    17461 tttgctaaaa gtgttgcctg aaacgatggc ttggcgcgtg atgttagggc tcgctgctgt
    17521 ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc gttttcttat
    17581 gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga caccaaaaga
    17641 agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga caaccggaac
    17701 ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg gtattggtgt
    17761 agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc cgctgatcgt
    17821 agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca tccaaggaat
    17881 cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca atcggcgaac
    17941 tttattgaaa ataggcggta gcgtgatggg actttcattc atcctaccag ctgttttagg
    18001 aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat gtatttatgt
    18061 cgcattttat tcttgcacat gggctccact gacatgggta atcataggcg aaatattccc
    18121 actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga ttggttcttt
    18181 cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa tcgttttcgg
    18241 catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg taccagaaac
    18301 tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt atcctaaacg
    18361 tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc aagaaataag
    18421 aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt ctcatgtttg
    18481 atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga gaggtggtga
    18541 aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga cgagtaaccg
    18601 taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt tcactgtttc
    18661 ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt tttcaatcgt
    18721 tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg tcaatgttgt
    18781 caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga tcgataggtc
    18841 atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag ctatttgatc
    18901 gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta ctgttttttc
    18961 agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca aaaaacgcac
    19021 attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt gaagatcgtc
    19081 gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt ctgctgccat
    19141 aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt cataagcagc
    19201 attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg ctaagtttgg
    19261 gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat aagcaatcat
    19321 tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta aaagcaatga
    19381 atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa aaatataatc
    19441 agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg ttttttcttt
    19501 cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat agccctcatt
    19561 taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata tttcttgcag
    19621 cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga tcttttgttt
    19681 tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt atttgtacgt
    19741 acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt tcttgtgctc
    19801 ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc cacgtaaagc
    19861 attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg ttcttctctg
    19921 gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta aacgagttgt
    19981 gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc gataatttat
    20041 gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg cccatggcat
    20101 agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg cgagtaagga
    20161 ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca ggtatagaaa
    20221 ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga taaagattag
    20281 aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt ccttgagaag
    20341 agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt cccttttcta
    20401 tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg cggtgatgat
    20461 gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac cattttccat
    20521 tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct tcatgagcct
    20581 gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc cttcttaatt
    20641 caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct gtatgtttat
    20701 atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc ttcatcataa
    20761 caaatttcct cacattttct ttattctaat tacattccaa gagtattttt ctatccctac
    20821 atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta ttttttctgg
    20881 ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt gctctccttg
    20941 atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg tgcgattgat
    21001 caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat aaggaacatt
    21061 ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata cttgaccttt
    21121 agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg cttgtccttt
    21181 agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac taatctttac
    21241 gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg tatcttccat
    21301 agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc ttttttccgg
    21361 ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac ttctcaaata
    21421 tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga atcgataagc
    21481 ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag tcaagtcttc
    21541 ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct cataagcttc
    21601 ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag agcttccaac
    21661 taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt catttgctaa
    21721 cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta gctcattccc
    21781 taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc ttaaatcact
    21841 ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt catcaattcc
    21901 ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga tccatttaaa
    21961 aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat caattttttt
    22021 tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg taaagtttcc
    22081 tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga catcttgtct
    22141 aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat agacgacact
    22201 tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta ccgtaaaatc
    22261 ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt catttcctat
    22321 agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa taaaaggagg
    22381 gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa tatttctcaa
    22441 aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg tgatatcttt
    22501 gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa gtaaaactac
    22561 atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa caagaaaata
    22621 aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct ttattccttg
    22681 aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca ttacataaaa
    22741 aacagcacat gtagcaggca cgatagtaat aacaggcaaa cacacgatta cccagagaat
    22801 acctaataag atgtaatcag aaaccacaat caactgtcca taaaatttag tctcaactat
    22861 tttcatcttc ctcactcttt cattccagac atagctgttc cttgaacaaa atatttctgt
    22921 gcaaaaatat aaaccactag taatggcagc attgaaataa ccgttccagc cattacaggt
    22981 ccataaaaag aaatgttctg ccctgtaaat aaagctaacc ctgctggtaa agttctcatt
    23041 tttgaactac ttgtcaatat caatggataa agcaagtcat tccaactatt catcagcgtg
    23101 aaaatcccta atgataaaag agtaggtttg ctcaatggca acatgatttt caaaaaaata
    23161 ttaaactcac ttaatccatc aatgcgtgct gcctcttcca aagaatctgg taaagtaata
    23221 aaaaaggaac gcatcatgaa gataccaaat gcagttgtca tacgaggaat gatcaaacct
    23281 gcataagtat caagcatgcc aagcaaattg acttcaataa atagaggaat catgaagact
    23341 tggaatggga tcatcattgt cagcaaaacg aaatagaaca aaactgattt tcctttgaaa
    23401 cgcatacgag caaatgcata ccccgcaaga gaatcaaaga atacagaagt gatcactgtc
    23461 cctccagcaa aaatcactgt atttttgatg taatctaata aagggatcgt tgtccaaacc
    23521 ttaatatagt tatctagggt atagctatct gcaaatattc taggcggaaa tgagataatg
    23581 tctttttcgt gtttaaaaga agaaataatc agccacaaaa aaggaaacgc aataaccaac
    23641 aatataacta gcatactaat cgttcgaatg atttttttac cactaactgc ttttcgacta
    23701 ttcacagtaa ctgtaatcga gctttctggt gccatacagg tttccctcct atttcatttg
    23761 tttttctttc cgtgacatgt acacattagt ggagatagct atagttgcaa caatgataaa
    23821 caaaaccgaa gacagtgctg atgcatagcc caattcataa ggtgctgtaa atcctcgact
    23881 gtaaatatat tgaacagctg tttctgtctt aaattgtggg ccaccagcgg ttgcaacata
    23941 tacttggtca aacacttgca tcgaaccaat cagattcgtt aaaatacaaa aacctaagga
    24001 gggaacgatc tgaggtagcg taatgttaaa aaactgctgt cgatttgttg caccatccat
    24061 ttgagcagat tcatacaaag agggagagat tccctgaata gcagtcaaga gaatcgtcat
    24121 agtaacacca aaattcttcc aaacagtcat gatggctacc gtcggcatag caagggttgg
    24181 atcgcgaaaa aattgaggga tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc
    24241 tacattcgga tcaagcaaca tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac
    24301 tggaatgtaa aatgtcgaac gacaaaaacg attgaatctg gtatttttag ataatgctac
    24361 tgctgctaat aaaccaacga taatttgtgt tggcgtttcg agtacggtga aatataaaga
    24421 atgaatcaac gaattaactg ctcgttcatc actaaatata cgtgtgaaat tttcaaaact
    24481 tacaaaattt cttgatgtaa agaaaatatt caaatctgta aaactaatag caaatgttcc
    24541 taaaagcggt atgacagtaa ataataataa aactaaaaaa gatggagtta aaaacaaata
    24601 cgcagcacga tcaggacgat tccaatattt ggtgaatccg ttcatatagt ccacctcatc
    24661 tttcattttg ataaaatata caatcttggt ttaaacgaat ctgaataaga tatccgaaca
    24721 atcgctgtga tttttgcaat aacacctatc tgcaaatact aggtcgatca ttcagatatc
    24781 tgtatcttgc caacagtcta tcctgtataa agttctgtac tatttttgca gtaattgatt
    24841 gatggcatca tctgcatcgt tcatgagtgt ctcaggatct tctcctgcta ataatttttc
    24901 aatcagtgga ttgatgatat cgttattgat cgcagagatt tgggtaaaac ctggtaaata
    24961 tggctctgca tagtccattt gtttcgataa ttcagataca ataggatcat tttttacttc
    25021 ttcatctttt gctactgaat ccaagtaggc aggaaaacca ttttcgatac tccatttttt
    25081 accaatttca gttgtattcc aatatttcaa aaactcatag acagcttctt ttttattatt
    25141 atctgtactt gccggaatgg catatcctac gccatctaaa atagctgttt ttttgtctga
    25201 atcactccct tgtggtaagg tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt
    25261 atttaaccat ggcccattga tttcaatggc tagttgacca gcattcatta aattgtccat
    25321 ttcggcgcca gaaatcgatt ctggaccagt cttgtctaca tggatcattt tttggatcgc
    25381 ttttaacgtt ttaaggttct caggagacgc aaattcagac ttagtataat catcgttcac
    25441 taatttaccg ccatttgcta acatccagtt atataacggt gcagttccat ccttattgaa
    25501 gacaaaacct gatacatttt tactactatc agttaatttt ggtgccattt ctgtcaattc
    25561 ttcccaagta gtaggtggtt gttcagtatc taatccagct tgtttaaaca aatcattatt
    25621 ccaatacata tacatgcctt gtacctgcat ggggacaaaa tactgtttac cttctacttc
    25681 acctaattca atagccgttt ctttaaaatc agatttatct actccatcat attcccagaa
    25741 atcgtctaat ggttgtaccg ctccgttttc tacatactgt gcaaaatctg gataatttaa
    25801 tgcaataaag tcaggtgctt ttttagaaga aatcgctgta ggaagttttt cattcaaatt
    25861 ggcccaagtc atgatatcca tttcaatttt gatgttttta tcatttgttt tattaaattc
    25921 attaacgatt tcttgcaata tttcaccatc agatgcagta aacccattcc aaaaagtcaa
    25981 agtcacttct ttatcagaat cagattctgc tgaacttccg ccaccgcaag ctcctaaaat
    26041 acctgttgca gctaccaaca aagagccaat taaccatttt ttacctttca ttttttgttc
    26101 ctccttcatc tgataaacga catctgacca cattccaaga tagcggtttc aataaacaat
    26161 tgattttccc atcttcagct tttacatttg ttgaatgact tggttttact tgttcacttt
    26221 taggaagatt agttgctttt gtatcaaatc cactcatttc agaaaaatca atcactccct
    26281 ctaatgataa ttcagacaag tgaacagtta gattcatttc ttcttctcca cggttaactg
    26341 caaatatgac aagttctttg tcatttatta cggctatact ttctaaatac ggcacttctt
    26401 caaaatcttc cgtcgcatag tttgctgaac gaatttgtgg gcttaacacc tctccttgtc
    26461 catacacagc agcttgcata aatggataat agatcgtctg tctccaagct tctccgtttg
    26521 tttctgtcct gattggtgca ataacgttta ccaattgagc aagacaagca atctttactc
    26581 tatctgcatg tttcaataaa gtgattaata gacagcctaa tagtaatgca tcttcaaaat
    26641 tatagttatc ttctagtaaa ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt
    26701 ccgctgaatg ataccaaata ttccattcat caaaggacag gttgatctgc ttcttactat
    26761 gtttcttcgc cttgactgta tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt
    26821 ctaaggaacg tgcaagataa tgagaaagat catttttttg gttgccataa tattgatgca
    26881 gagacaaata atctatttga tcgtacgctt gatcaagaac tgttaactcc caatcaccaa
    26941 atgtaggcat ttgccgattc gaacttccgc ataaaaccac ctcaattgaa tcatctacga
    27001 gtttcatcgc cttagccgtt tcctctgcta accgtccata ttcttccgct gttttatgtc
    27061 caatctgcca aggaccgtcc atttcattcc ctaaacacca tgtcttaatt ccaaaagggc
    27121 tctcttgacc attcgaacgc cttaaatcac tccaataagt tcctttagga aaattacaat
    27181 attcgactaa attacgagct gcatctactc cacgagtacc cagattgaca gccatattga
    27241 tttcggcccc cacatgctga caccagttat aaaattcatg aatcccaact tcattcgttt
    27301 caattgtccg ccaagctaaa tccaaccttc taggtctatt tgctatgggc ccaatactgt
    27361 cttcccaacg aaatcctgac acaaaatttc caccaggata tctaatcatg ggaatgttca
    27421 attcttttac caagtgaatg acgtcttggc gaaatccact tttattagct gatggatgat
    27481 ctggctgata aatcccttca tatactgctc gtcccatatg ttcaataaat gaactgtaca
    27541 accgcggatc aactttgcct acacgcttga cactactaat caacattgtg gcttccataa
    27601 ctgacctcct ttaatttata catgaataat acacctcgtt gaaagcgttg tcaataatat
    27661 aaatgttaaa cttacttatt ttttatcgat tttaattaac caaaatatta ataatgatta
    27721 aaacaaataa tttaatataa cttttattaa caaatatgtt aatatataag aaaaaccttt
    27781 taagaggtga tatatatgca actacaaata aacgaagaat ccttaccggt atatgaagca
    27841 ttagctagta aaacaaggat caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc
    27901 aaagatttgg caaaagaact tggagtgagc agtgctatca cgacgatgca cgtgaaaaag
    27961 ctagaggaag caaacatcat caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt
    28021 cgtgtcgata aaatcgatat tagttttcca gaaaaaatat tcaatgcttt tgatacgaaa
    28081 gaaacatcga tacctattgg tcactataca aattatgcaa ttgaaccaac atgcggttta
    28141 gctacgatcc atgattttat cgggaaagtg gatgaaccac gctattttat ggatcctcga
    28201 aggatggatg cccgcattct ctggtttacc agcggatttg tcgagtatca ggcacctaat
    28261 tttttaaata cagaggatac attggagatg ttggaagttt ctgtggaaat ttcttctgaa
    28321 tttccttttt caaatgacaa ttggccttca gatatcactt tcagcctaaa tggtgtggag
    28381 ctaggaacat ggacgagccc tggagatttt gctgatatcc gcggtaaata tacgcctgat
    28441 tggtatccag ataatcttaa tcaatacggc ttgctgaaga cgatccgaat taccaaacac
    28501 ttgacgaata tgaatggaga acccttgtca aacatcacga tcaacgacat ccctaaagag
    28561 caagacactt ggcatttgcg aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt
    28621 actttatttg gtaaaggatt cggtaattac gatcaggata tcaaattaaa agtttactac
    28681 agctgatatt tttcttttct attttataaa acaaaaacag ccaatcccta tgaagggacg
    28741 gctgtttttg ctttattgtt atttccagaa atcatccaaa acggaaattg gcaaatgtcg
    28801 tttatgttgt gtcttgttcc accatgcttc gattttttct tgtgcttgag cgggaacttc
    28861 ttttccttct agataatcat caatttcatt atatgtgaca cctagtgcga cttcatctgc
    28921 aatcattggt ttcccatctt caagatctgc agtcggaatt tttgtgtata atttttccgg
    28981 tgcattcaat gcttgaagca attgtttccc ttgtcgtttg tccaaacgga ataatggcaa
    29041 aatgtcggct ccaccatcac caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg
    29101 atctgtccct aatacggctc ctgctctttc tccagctacg gcatattgcg taatcatacg
    29161 ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt cccacacctg cttcgcttaa
    29221 tacttgtact tgtgcatcaa cagctggttt gatatttact cgcagactaa catcgggctg
    29281 gataaagtca agagcagctt ttgcatcttc ttcatctgct tgttcaccat aaggaagacg
    29341 aacagcgata aattgatagt tttcatcttt tgtttcttca cgcatttctt ccatcgttag
    29401 ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg ctgataccta gtacaaaagt
    29461 tttaagaaac ggatattttt tcaaatatgc tttcataaag tcaatacttt tacgtatttc
    29521 ttcttttgga tcaattgttg gtttgacacc taattcattg ataatttctg tctgcaggct
    29581 catttattct cctcctatgc ttctgtatga agttctgcta cgcttttttt cattcgttcc
    29641 atgatagcca ttttatggtt ccagcattca gtagacagat cgactgggta tttttgcgga
    29701 ttcaagtctc gtttatactc ttcccatagt gaatctagat tttcttttgt atattctttg
    29761 atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt cgataaagat atcttgtaaa
    29821 actgggcgag ctgtaaagtc tcgaacagtt ttattgataa acgtatgcac tggatggaac
    29881 atgaagatag cttcttcttc tcgagggtct tcatcccata atgtcacgta gtctccttca
    29941 gatttcccat caaaatttcg agtgatgcgc cacacttgtt ttttccctgg agtagttact
    30001 ttttctgcat tgcttgataa cttgatcgta tccatcattt ttccttcatc atcttcgatc
    30061 gatactagct tgaatactgc accaagggct ggctgatcat aggctgtgat caattttgtc
    30121 ccaacacccc atacatcaat tttagctttt tgcatcttta aattcaaaat cgttgcttca
    30181 tccaagtcat tcgaagcata gatttttgct tccgtaaatc cggctgcatc taattgttcc
    30241 cgaacacgct tagatatata ggccatatct ccactgtcga tgcgtacgcc ttggaagttg
    30301 attttgtcac ctaattcttt cgccacgcga atcgcacttg gcacacctga tttcaacgta
    30361 tcataagtgt cgactaagaa cacgcagtct ttatgggttt tagcgtaggc cataaatgct
    30421 tcataatcat ttccataaga ttggactaac gaatgagcat gggtcccgct agctggtatc
    30481 ccaaatatct tcccagcacg tacattgctt gttgcatctg ctccgccgat atatgccgct
    30541 ctagttcccc agactgctgc atccagttct tgtgcccgtc tagttccaaa ttccaatagt
    30601 ggatcttctc cgatgacaga tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa
    30661 ttgaccatat tcaacagtgc tgtttctact aattggcagt gcgctaaagg cccttccact
    30721 tggatcaatg gttcatttgc aaaaactagt tctccttcac gagcagaacg aactgttgct
    30781 ttaaattcaa aattttgtaa atactccaaa aaaccttcag gatagacttc taatccacgc
    30841 aagtaagcaa tatcggaatc attgaacgtc agattctcta aataattgac taaccgttcc
    30901 aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt ctcgaaagta acattcaaaa
    30961 acagcatgta aatcagctcg acctaattcc cagtatgtct gcatcatatt gatttgatat
    31021 agatccgtat gcagcgtcaa actgtcgtct gggtaagtat tattcatctt gtattactcc
    31081 tcttggcaag ttttcatttc ctacccatta taacaaaaaa aattaatagg aaaagaaatc
    31141 ttccattttt tctgaaaaag cacatgtttt acggatattt cttgctaaaa atgaactcca
    31201 tctgctaaaa ctatcctttc ttattggtat ttaacctaaa cgagaaagaa tacttatttt
    31261 attcaatgta tccgagacac gaagaaatta gcgttttcta tttttcttac ttgataaaat
    31321 aagtagattt tcctactaca atcgtagccg ttaagatacc gatgattgaa caaatataac
    31381 caaatgtctc tacaacataa tcaatggcaa aagtttttga agcaataata cgtctttcct
    31441 tttttgcttc ttatccccaa agaaacggat ataacttatt atgtattgaa tttttttgga
    31501 aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac
    31561 gtttttaaaa agatacaaag taagaaagca gctgtcaata gaatggaaac aaaaatggcg
    31621 agacatcaat cagtctcgcc ttgttttt
//
LOCUS       DARDHU010000020        30227 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000020 DARDHU010000000
VERSION     DARDHU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.020"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..369
                     /locus_tag="SDD03_000999"
     CDS             1..369
                     /locus_tag="SDD03_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG4303464.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="SDD03_001000"
     CDS             549..1490
                     /locus_tag="SDD03_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG4303465.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="SDD03_001001"
     CDS             1559..2290
                     /locus_tag="SDD03_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG4303466.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="SDD03_001002"
     CDS             2434..4614
                     /locus_tag="SDD03_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG4303467.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="SDD03_001003"
     CDS             4634..5116
                     /locus_tag="SDD03_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG4303468.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="SDD03_001004"
     CDS             5132..5893
                     /locus_tag="SDD03_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG4303469.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="SDD03_001005"
     CDS             6024..7358
                     /locus_tag="SDD03_001005"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG4303470.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="SDD03_001006"
     CDS             7665..8150
                     /locus_tag="SDD03_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG4303471.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="SDD03_001007"
     CDS             8143..8826
                     /locus_tag="SDD03_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG4303472.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="SDD03_001008"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="SDD03_001008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG4303473.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="SDD03_001009"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="SDD03_001009"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG4303474.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="SDD03_001010"
     CDS             12442..13071
                     /locus_tag="SDD03_001010"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG4303475.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="SDD03_001011"
     CDS             13123..13455
                     /locus_tag="SDD03_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG4303476.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="SDD03_001012"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="SDD03_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG4303477.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="SDD03_001013"
     CDS             14281..15654
                     /locus_tag="SDD03_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG4303478.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="SDD03_001014"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="SDD03_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG4303479.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="SDD03_001015"
     CDS             16709..17149
                     /locus_tag="SDD03_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG4303480.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="SDD03_001016"
     CDS             17293..17994
                     /locus_tag="SDD03_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG4303481.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="SDD03_001017"
     CDS             17991..19112
                     /locus_tag="SDD03_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG4303482.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="SDD03_001018"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="SDD03_001018"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG4303483.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="SDD03_001019"
     CDS             20491..21399
                     /locus_tag="SDD03_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG4303484.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="SDD03_001020"
     CDS             21434..21706
                     /locus_tag="SDD03_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG4303485.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="SDD03_001021"
     CDS             21717..22763
                     /locus_tag="SDD03_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG4303486.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23014..25623
                     /gene="clpB"
                     /locus_tag="SDD03_001022"
     CDS             23014..25623
                     /gene="clpB"
                     /locus_tag="SDD03_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG4303487.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25774..26460)
                     /locus_tag="SDD03_001023"
     CDS             complement(25774..26460)
                     /locus_tag="SDD03_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303488.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26438..26932)
                     /locus_tag="SDD03_001024"
     CDS             complement(26438..26932)
                     /locus_tag="SDD03_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG4303489.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26952..28172)
                     /locus_tag="SDD03_001025"
     CDS             complement(26952..28172)
                     /locus_tag="SDD03_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4303490.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28319..30154
                     /gene="lepA"
                     /locus_tag="SDD03_001026"
     CDS             28319..30154
                     /gene="lepA"
                     /locus_tag="SDD03_001026"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG4303491.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10133 a         5387 c         6522 g         8185 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aatattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
    22921 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
    22981 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
    23041 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
    23101 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
    23161 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
    23221 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
    23281 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
    23341 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
    23401 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
    23461 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
    23521 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
    23581 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
    23641 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
    23701 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
    23761 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
    23821 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
    23881 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
    23941 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
    24001 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
    24061 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
    24121 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
    24181 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
    24241 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
    24301 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
    24361 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
    24421 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
    24481 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
    24541 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
    24601 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
    24661 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
    24721 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
    24781 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
    24841 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
    24901 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
    24961 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
    25021 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
    25081 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
    25141 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
    25201 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
    25261 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
    25321 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
    25381 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
    25441 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
    25501 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
    25561 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
    25621 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
    25681 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
    25741 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
    25801 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
    25861 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
    25921 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
    25981 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
    26041 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
    26101 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
    26161 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
    26221 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
    26281 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
    26341 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
    26401 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
    26461 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
    26521 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
    26581 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
    26641 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
    26701 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
    26761 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
    26821 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
    26881 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
    26941 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
    27001 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
    27061 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
    27121 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
    27181 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
    27241 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
    27301 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
    27361 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
    27421 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
    27481 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
    27541 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
    27601 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
    27661 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
    27721 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
    27781 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
    27841 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
    27901 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
    27961 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
    28021 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
    28081 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
    28141 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
    28201 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
    28261 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
    28321 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
    28381 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
    28441 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
    28501 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
    28561 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
    28621 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
    28681 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
    28741 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
    28801 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
    28861 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
    28921 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
    28981 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
    29041 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
    29101 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
    29161 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
    29221 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
    29281 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
    29341 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
    29401 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
    29461 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
    29521 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
    29581 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
    29641 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
    29701 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
    29761 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
    29821 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
    29881 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
    29941 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
    30001 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
    30061 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
    30121 tctgaaaatg gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa
    30181 aaaataaacc atgtttacat ggatttgcca ccggtttgcc accggtg
//
LOCUS       DARDHU010000021        30010 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000021 DARDHU010000000
VERSION     DARDHU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.021"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(241..1968)
                     /locus_tag="SDD03_001027"
     CDS             complement(241..1968)
                     /locus_tag="SDD03_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG4303492.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1961..3154)
                     /locus_tag="SDD03_001028"
     CDS             complement(1961..3154)
                     /locus_tag="SDD03_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4303493.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3341..3985)
                     /locus_tag="SDD03_001029"
     CDS             complement(3341..3985)
                     /locus_tag="SDD03_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4303494.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4079..4213)
                     /locus_tag="SDD03_001030"
     CDS             complement(4079..4213)
                     /locus_tag="SDD03_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG4303495.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4215..6347)
                     /gene="feoB"
                     /locus_tag="SDD03_001031"
     CDS             complement(4215..6347)
                     /gene="feoB"
                     /locus_tag="SDD03_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG4303496.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6344..6817)
                     /locus_tag="SDD03_001032"
     CDS             complement(6344..6817)
                     /locus_tag="SDD03_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG4303497.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7218..7442
                     /gene="nrdH"
                     /locus_tag="SDD03_001033"
     CDS             7218..7442
                     /gene="nrdH"
                     /locus_tag="SDD03_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG4303498.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7601..9760
                     /gene="nrdE"
                     /locus_tag="SDD03_001034"
     CDS             7601..9760
                     /gene="nrdE"
                     /locus_tag="SDD03_001034"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG4303499.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9810..10775
                     /gene="nrdF"
                     /locus_tag="SDD03_001035"
     CDS             9810..10775
                     /gene="nrdF"
                     /locus_tag="SDD03_001035"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG4303500.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10864..12003)
                     /locus_tag="SDD03_001036"
     CDS             complement(10864..12003)
                     /locus_tag="SDD03_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4303501.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12312..13025)
                     /locus_tag="SDD03_001037"
     CDS             complement(12312..13025)
                     /locus_tag="SDD03_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG4303502.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13279..13980)
                     /gene="nagB"
                     /locus_tag="SDD03_001038"
     CDS             complement(13279..13980)
                     /gene="nagB"
                     /locus_tag="SDD03_001038"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG4303503.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14214..15746
                     /locus_tag="SDD03_001039"
     CDS             14214..15746
                     /locus_tag="SDD03_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4303504.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15937..17139)
                     /locus_tag="SDD03_001040"
     CDS             complement(15937..17139)
                     /locus_tag="SDD03_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4303505.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17221..17910)
                     /locus_tag="SDD03_001041"
     CDS             complement(17221..17910)
                     /locus_tag="SDD03_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4303506.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18016..18384)
                     /gene="rplL"
                     /locus_tag="SDD03_001042"
     CDS             complement(18016..18384)
                     /gene="rplL"
                     /locus_tag="SDD03_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG4303507.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18437..18940)
                     /locus_tag="SDD03_001043"
     CDS             complement(18437..18940)
                     /locus_tag="SDD03_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG4303508.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19144..19271)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19350..20039)
                     /gene="rplA"
                     /locus_tag="SDD03_001044"
     CDS             complement(19350..20039)
                     /gene="rplA"
                     /locus_tag="SDD03_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG4303509.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20143..20565)
                     /gene="rplK"
                     /locus_tag="SDD03_001045"
     CDS             complement(20143..20565)
                     /gene="rplK"
                     /locus_tag="SDD03_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG4303510.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20908..21576
                     /gene="sdaAB"
                     /locus_tag="SDD03_001046"
     CDS             20908..21576
                     /gene="sdaAB"
                     /locus_tag="SDD03_001046"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG4303511.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21589..22461
                     /gene="sdaAA"
                     /locus_tag="SDD03_001047"
     CDS             21589..22461
                     /gene="sdaAA"
                     /locus_tag="SDD03_001047"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG4303512.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22552..23571)
                     /locus_tag="SDD03_001048"
     CDS             complement(22552..23571)
                     /locus_tag="SDD03_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG4303513.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23744..24649)
                     /locus_tag="SDD03_001049"
     CDS             complement(23744..24649)
                     /locus_tag="SDD03_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4303514.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24708..25253)
                     /gene="nusG"
                     /locus_tag="SDD03_001050"
     CDS             complement(24708..25253)
                     /gene="nusG"
                     /locus_tag="SDD03_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG4303515.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25354..25524)
                     /gene="secE"
                     /locus_tag="SDD03_001051"
     CDS             complement(25354..25524)
                     /gene="secE"
                     /locus_tag="SDD03_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG4303516.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25548..25700)
                     /gene="rpmG"
                     /locus_tag="SDD03_001052"
     CDS             complement(25548..25700)
                     /gene="rpmG"
                     /locus_tag="SDD03_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4303517.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25862..26506
                     /locus_tag="SDD03_001053"
     CDS             25862..26506
                     /locus_tag="SDD03_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4303518.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26562..27065)
                     /locus_tag="SDD03_001054"
     CDS             complement(26562..27065)
                     /locus_tag="SDD03_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG4303519.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27078..27124)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27662..28627
                     /locus_tag="SDD03_001055"
     CDS             27662..28627
                     /locus_tag="SDD03_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG4303520.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28726..29898)
                     /locus_tag="SDD03_001056"
     CDS             complement(28726..29898)
                     /locus_tag="SDD03_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303521.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29885..>30010)
                     /locus_tag="SDD03_001057"
     CDS             complement(29885..>30010)
                     /locus_tag="SDD03_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303522.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9138 a         6178 c         5357 g         9337 t
ORIGIN      
        1 ttttgagaat gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga
       61 tcgatctctt tgactcgcac ccataaattc ataatagttt gtcggcgtct ttggacgttt
      121 cgaagatgag ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag
      181 cttccaaatt tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt
      241 ttattttttc cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg
      301 ctgtggatct tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca
      361 atggaaattg tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac
      421 catcgtatgg atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag
      481 atcatatttg gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt
      541 tttgatcgct tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca
      601 gtctttcaag ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc
      661 tggctcgatt cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac
      721 atccatgtag gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc
      781 ttcgacttga tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga
      841 aaccatggca tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg
      901 cgtcatcgtt acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc
      961 aacttctgta tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga
     1021 aaaactccct ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc
     1081 ttgacgtttt ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt
     1141 caaatgctgt gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac
     1201 aaatccattc ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg
     1261 tgcaccgatc atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat
     1321 ccctgccatg atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact
     1381 tgtgctcccg aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa
     1441 attcgtaaaa cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac
     1501 catcccgata ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg
     1561 cataaagtca aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat
     1621 ccctaatggt accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt
     1681 attcatcaaa tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa
     1741 caacgtaagt cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat
     1801 aaacaaagga gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc
     1861 aatcgtttgt aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt
     1921 ttctacccag tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct
     1981 cgcttgtttc tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc
     2041 catcctctac aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta
     2101 ccaccatgtc tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt
     2161 tttgcttcct agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc
     2221 tgtcaacagc catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg
     2281 gtccatcaga ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca
     2341 ctttggaacg atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca
     2401 atatttcttc acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt
     2461 ccccaatccg taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat
     2521 tggcttgcag gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg
     2581 agaacgcttc gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa
     2641 cccgttgttg catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac
     2701 tagaattttc taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga
     2761 cttctaaccc gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt
     2821 ggaaaaccat attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg
     2881 atacatcttc tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata
     2941 gacggatcaa tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt
     3001 cttttacgtc aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct
     3061 ccgttttcgg ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata
     3121 tctttgttaa atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt
     3181 catttttctt tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa
     3241 aaatgaaaga aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa
     3301 aggtttagcc aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct
     3361 ccgggtaaaa aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga
     3421 tgtcccctgc cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag
     3481 aaattgcgat aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt
     3541 ccagatgcat tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat
     3601 gtattcgctt cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat
     3661 cttgttgctt ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat
     3721 cttgatattg caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc
     3781 catgccgtac ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct
     3841 caggagaagc attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt
     3901 tatatcggtt ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc
     3961 ggacgatttt tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc
     4021 ttactatgct ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc
     4081 atcgcttgat ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac
     4141 cggattttaa acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata
     4201 agaatgtcgc catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg
     4261 gattttggct gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac
     4321 gtccctaacg ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta
     4381 actaaataag ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc
     4441 atttcccgat ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct
     4501 aacaatgaat aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg
     4561 ccattttcgg atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct
     4621 ttagcgacca atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc
     4681 tgaaaaacaa ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca
     4741 ggttgagcat aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata
     4801 atcgttcctg cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct
     4861 gaccattgag gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca
     4921 aacaaacgtg tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa
     4981 taggcagaag gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata
     5041 atcggcaact ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct
     5101 tgctcgtttt caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc
     5161 agcataggaa tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca
     5221 aaagcgatcc tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata
     5281 atcaattgcg gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc
     5341 agctcctgca tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt
     5401 aaccatgtac caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga
     5461 atagccagat aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga
     5521 ttcgttgcaa tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca
     5581 acacagagtg tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca
     5641 ctatcgtcgt ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga
     5701 gaaactgcta tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca
     5761 taccagcgtt tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct
     5821 tctaaacgtg gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc
     5881 tgaatctttt cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact
     5941 ggtacaccaa gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg
     6001 acatccatca tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt
     6061 aaataaagat ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt
     6121 tcctgaacca aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag
     6181 atgccgggta ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt
     6241 tctactgtta cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac
     6301 aagctagttt tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc
     6361 tttgtatcaa gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg
     6421 tcagttcgat tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg
     6481 tcaatcccat atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga
     6541 tcccagtctc tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct
     6601 ttttattgac aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta
     6661 ccagtaaaat ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga
     6721 tcccgatatt ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat
     6781 aaacgaaatt ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata
     6841 aatcatgtat taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa
     6901 tgagaatcat tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat
     6961 attatgagcg aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt
     7021 tttccacaat tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct
     7081 cttttttcaa aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt
     7141 tgttgtttga cttttttact aagagtttta tactatctat tatagataat aagaatgtgt
     7201 tagaggaggc actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta
     7261 agatggtcaa acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg
     7321 aacagcctga agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta
     7381 cttctgatgc agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct
     7441 aacatgacta aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag
     7501 tttttgtcgc aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa
     7561 tcaagcatca tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca
     7621 agatgtcagt tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc
     7681 tttgaataag gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac
     7741 gatgactttt ctatctatta tggaaaaaat caattacttg atcgatcaag aatatataga
     7801 agcagaattc attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga
     7861 cgagcaacat ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc
     7921 gttgaaaacc aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa
     7981 tgcgctttac tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat
     8041 ccatcaacga tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg
     8101 cgaattggtg tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc
     8161 aatcaactct gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa
     8221 tcttcgtgaa gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc
     8281 agtcatgaaa ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg
     8341 agctggcgtg gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa
     8401 aaaagaaaat gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc
     8461 tgataaattt tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag
     8521 tgtcgaaaaa gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca
     8581 tctagttgca aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga
     8641 aatctcaaaa ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa
     8701 ccgcgagaat ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca
     8761 agttcaaacg ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacggatat
     8821 ctcatgtaac ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca
     8881 atctgtgcgt acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt
     8941 cgtaccaccg atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg
     9001 cttacacact tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt
     9061 tacaaatatt tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc
     9121 tcgtgaaaga aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta
     9181 tttcgaaaaa tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt
     9241 tgaaggcatt tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa
     9301 agatggactg tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat
     9361 caacgatacg agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa
     9421 gaaaatcggt aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta
     9481 cacttctgct tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa tggcacaaca
     9541 acacgtcgat caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat
     9601 ttatgaatgg aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta
     9661 cgctttccac aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga
     9721 agaaatcgga agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt
     9781 attcatattt taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact
     9841 ggaatgaaat tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt
     9901 ggctagatac acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat
     9961 tggaaaaaga tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat
    10021 ctgaaagtgg catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt
    10081 tgaacaatat tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta
    10141 cactgaatac gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc
    10201 tgcaaaaaaa agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga
    10261 aaattgccag cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt
    10321 attatttagg aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag
    10381 atgaatctgt acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac
    10441 ctgaagcaga acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg
    10501 aaaacgaaga acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca
    10561 agacattcct acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct
    10621 ttccagatac tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa
    10681 gcaaccatga cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga
    10741 tgaaagacga cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt
    10801 tcgaaaaata aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta
    10861 tgcttattca cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc
    10921 cattcttctc aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata
    10981 gacaggtatg caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac
    11041 caagatgatc gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa
    11101 atttccatct aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg
    11161 agagataaac aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg
    11221 gataatattc gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat
    11281 tctcattgcc aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat
    11341 ataggcagat aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc
    11401 tttcggaaag aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag
    11461 caaaataaat ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt
    11521 tgagacactt gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt
    11581 tgctgatgct aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc
    11641 atttaaccat ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt
    11701 ctgttccatt aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac
    11761 caaaaacgtc aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac
    11821 gattggatcg aacatataat agataaaacc agcaacaagc aatggtaaaa aaactgccga
    11881 aagcattttc aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat
    11941 gatcgtgagt actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg
    12001 catacatctc ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc
    12061 gctgagattt tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac
    12121 gagtacttaa acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt
    12181 tccaaataaa cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat
    12241 ataaggaact atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa
    12301 cctcagatca tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg
    12361 gacaactcct cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata
    12421 tttttgtaca ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg
    12481 atatttttct cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat
    12541 tggatttcta actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca
    12601 atagcaatcc cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc
    12661 cctaatccag caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc
    12721 gtgacgatga tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc
    12781 cttgcttcat ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat
    12841 gcaccactag cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa
    12901 atatgcgtac gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg
    12961 ccaaatagca tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca
    13021 ttcatctaaa caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg
    13081 caatttttac ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat
    13141 gaataaacgg tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg
    13201 gagctatttt tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct
    13261 ttagcctact attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac
    13321 attgtcgtga ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat
    13381 ccctttgatt gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt
    13441 cataattgaa ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa
    13501 gaatcgtttg tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc
    13561 aaatgaagca ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg
    13621 gatatcgatt ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc
    13681 ttctgctttt ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac
    13741 atccatgaaa tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa
    13801 gtttacagaa gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt
    13861 aactggtgtg cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt
    13921 gatgatttca aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat
    13981 agtgattgct cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac
    14041 aataattagt atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg
    14101 tgttaggttt cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt
    14161 gattcactgt ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta
    14221 tactgacatt aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg
    14281 atctgcaatt aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa
    14341 gtacattgat taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc
    14401 aaaaaaatat tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa
    14461 tcgaagaatt tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt
    14521 ttctttacca aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac
    14581 tccaaacgga attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga
    14641 gcagcgggtt ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg
    14701 gacaacgttc aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct
    14761 tagatgaacc aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc
    14821 aggattttga cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca
    14881 ccacccatat cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac
    14941 aagcattgaa acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac
    15001 aaaaacaaat cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa
    15061 cgatggcaaa aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt
    15121 cagcaagtaa agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg
    15181 aaacaactga tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca
    15241 tccgctttgg tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat
    15301 taaaaacact gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca
    15361 caaaaatcaa ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac
    15421 aatatttaag cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta
    15481 aagcagggtt agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac
    15541 aaactaaggt gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg
    15601 aaccgacgaa ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat
    15661 ttccaggaac agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac
    15721 gagtcattga gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt
    15781 gacaaaagtt ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc
    15841 ggtattagct caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta
    15901 cttgatgcag aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct
    15961 ctttccttta aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct
    16021 aaccgttaca cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc
    16081 cgcacctcca ataaagtaca taaacataaa tacagtatta tttcggttgc ttgctgcttc
    16141 acctagccct tgtactcgtg tttgattggc gacttgacca aattgattcc cgacgtctaa
    16201 taaaataata gctaaaagca aaatggccaa atggcttccg ccaaccaata aagcaataaa
    16261 gcttattgtt tgcataacaa gtcccatata cacgattttt ctttcagaat aagaatctgc
    16321 cagtcgtccc acgataggtg cagcaactgc tccagacaaa ccaaaaatcg ctaatatccc
    16381 tgcttcgaat gttccccaat tgtaggcggg actgctgata taaaaaatca acgtagacca
    16441 aaagatcgaa aatgttccaa acatgaaaaa tccactgact gccgcttctc ttaatagacg
    16501 ttgagaagca actaaatgcg gcaaactttt aagtgaatca aggtatgaaa cagctgtact
    16561 actttgctgg acagaacgcg gcattttcca gtaaagcaga agtgtaagaa ttccgactgc
    16621 acataaagca aataaataga tcatccgcca agaaaaaaga ctggcaacaa taccagatac
    16681 cgtccgagaa agcaaaatcc ccacgagtaa accgctaagc aatgtaccca tgaccttccc
    16741 tcttgctttt ggtcccgcta gtaccgcacc gtaaggaatg atgatttgcg gcacgatcga
    16801 aagtaatcct attaaaaaag aagcgcatgc aaataacgta aacgaaggcg caaaaaaagc
    16861 ggccaacaga gagacggcag ataacgctgc cattcgaatg atcaattttg gacgatccac
    16921 cacgtcccct aaaggaacta aaaacagtaa ccctaacgca tagcctaatt gggtcaacat
    16981 cgtcaatatc ccgactgcac ttgtgctaac agataaacta gctgctactt ttgttccaat
    17041 cggctggatg taatacatat tggcaacaac aacgccacaa gtgattgata atagtaaaat
    17101 aagcgaagtt gttaaaggtt ttttttcatt actcttcatt gatttccccc atttcctatc
    17161 ttagtttaca acttatggaa aagattggaa atagaaaaat acaactattc gccacactta
    17221 ttatttgtgc ttttgttttc cttgtttttt cttgcgatct ttataaatga agacaactaa
    17281 ccctactagc acagtaacac ttgcaaccaa cgtgactgca gttttgttat atccgtgaaa
    17341 cagagcttct ccaccacacg catacaacac agacgaaggg atgactccaa tcacgatcga
    17401 caatatgatt tgctgtagag gcaatttcat cgcatctgca gcaaaattga ccatagaaga
    17461 tggaataacc ggtatcatat agcctaacat tacacctatt tttggatgtt tcatttgtcg
    17521 gattgcttgc acccaatgat tcgtttcggt tttcttatct aaaaatttta agcgatgcat
    17581 aaggaaaatt gcagtgatgt taccaagagc attacccaaa atgttgatga tcgcacctgt
    17641 cagtggtcca aagctcaaac cagcaatgac tccaactact gaagtaggaa tacctggaac
    17701 agcacacatc accgctgtca atacgatcaa gagtatcgat gcaaaagtcc catgcgaacg
    17761 aatatgtgcg agtagctctg ccttactcgc tctcggattt aaaaataatt ggatgtctgt
    17821 ctgatattct aaatacaacc gatagcccac taccagtatc agaatgattc ccaacgagat
    17881 caatatcaat cggattattt tctttttcat atttcctcct gaaaaaaatc tatattcatt
    17941 aaataatagt ttaacataaa aacaaaaaag caccgaaatc caacatagga tctcagtgct
    18001 tttaaatagt aaaaattatt taacagttac agatgcgcca acttcttcaa gtttagcttt
    18061 caattcttca gcttcttctt tagatacgcc ttctttaact ggtgcaggag cgccatcaac
    18121 tacagcttta gcttctttca agcctaaacc agttgcttca cgaactgctt tgataacttt
    18181 aactttttgg tctccagctg cagttaattc tacagtgaat tctgtttgtt cttcagcagc
    18241 agcagcgcca ccagcagcag ctacagctac aggagcagca gcagatacgc caaattcttc
    18301 ttcaatagct ttaactaggt cgttaagttc taggatagtt gcttctttta actcagcaac
    18361 gatgttttca atgttcaatg ccattataat ttcctccgtt taattgttct tttttattta
    18421 atgctttatg cggcaattaa gctgcatctc catcttcttt gtctgccact gctttgacag
    18481 cgtaagccac gttgcggact ggcgcttgta gtacagatag caacatagat agaagtcctt
    18541 cgcgatttgg caattttgcc aatgctgtga tttcttccaa tgaagagact ttaccttcga
    18601 tgataccgcc tttgatttct aatgcttttg cttctttagc aaattcgtcc atgattttcg
    18661 ctggtgcgac tacatcttcg ttactgaatg caacagctgt aggtccagtg aatacttcgt
    18721 ccatgccttc aaggccagct tttttagctg cacgtgaaag aatagaattt ttgataactt
    18781 tcatttctac gttagcatca cgaagttgtt tacgtaaacg tgttacttca tcaactgtta
    18841 atccgcggta gtcaacgact actactgatg cagctgctgt aaatttttcg cttacttcat
    18901 caacgattgc tgcttttttt gcgataattg cttcactcat ttttgagttt cacctcctga
    18961 attttgatgg aagagttttt tgcactgaaa aggcaagtca cttttatagt tactttgctc
    19021 aaccagtatt attcatcacc tattcacttc gttcatagtg attctactcg ctttgctcga
    19081 aaggaaactc ctttcgcttt gctcaaccag tattgttcat catctcttca cttcgttcat
    19141 agtgattcac aataaaaaac tctatgccac cgcagacata gagggactat tgaatatatt
    19201 caataaacgt cctcggtagg aaattaaggc ttgatgccac ctactgtctt cggtacaagt
    19261 taattattaa cctcaactag cttagcatgc taaactagtt gagtcaagaa ttatttttca
    19321 gttggatcaa tcaacattct aatatttaat tagaaagaag cttggtcaac atggatacca
    19381 ggtccaaatg ttgttgtgat cgttacattt ttcatgtatt gacctttagc tgctgatggt
    19441 ttagctttta gcaatacatc atggattgct gcaaagtttt caactaattt ttcatcgtca
    19501 aatgaaactt ttccgattgg tacatggata tttccagctt tgtccacacg gtaagttact
    19561 ttaccagctt tcacttcgtt tactgctttt gttacgtcca ttgtaactgt accagtttta
    19621 gggtttggca ttaagccttt tggtcctaat actcgtccta gacgacctac agtagccatc
    19681 atatcaggtg ttgcaacaac tacatcaaag tcaaaccatc cgttttggat tttttgaacc
    19741 atgtcgtcgt cacctacgaa atctgcacca gctgcttcag cttcttttgc tttgtctcct
    19801 ttagcaaaaa ctaaaacagt ttgtgtttta ccagttccgt ttggtaatac aacagcacca
    19861 cggatttgtt ggtctgcttt tttagggtct acgttcaaac ggtatgcaac ttcaactgtt
    19921 gcatcaaatt ttgcaatgtt tgtttctttt gctaaagcta ctgcttcggc aacaggataa
    19981 actttacttg aatcaacttt ttttaatgca tcttgcattt ttttgctctt tttagccatt
    20041 ttggttcctc cttgattgtg gttataacgg ttgtacctcc cacgtgttct atatctttca
    20101 atatagagcc aactgagaag ctgctgtctt agggtgtata gattattcta cagtgatccc
    20161 catgcttcgt gcagttcctt caaccatgcg cattgctgct tcaacgttag ctgcgtttag
    20221 gtcttccatt tttaattcag cgatttctct tacttgatcg ctagaaactt tcgcaacttt
    20281 atttttgttt ggttcgcctg aacctttttc gattttagct gcttttttca atagaacagc
    20341 agctggcggt gtttttgtaa cgaatgtgaa tgaacggtct tcgtatacag aaataacaac
    20401 cggaatgatc aaacctgctt gatcagctgt acgagcgttg aattcttttg tgaatcccat
    20461 gatattgata cccgcttgac ctagtgcagg acctactggt ggagctggag ttgctttacc
    20521 tgcaggaatt tgcaatttaa cgattttttc tactttcttt gccacgagac atacctcctt
    20581 aagtccgtga tgtggttaat tggagatgca tatttctcct cccacgtttt atctgatctg
    20641 aaaaaatcca gacaaataat gtgcaaaaca atgcacactg gtacagtata gcaaatttat
    20701 ttgttttttc aagatttttt tctgctattt ttacattttg cttgaataaa cgctaaattt
    20761 attctaaaaa aacaacaatt tttcgttctc atttcttctt ttatgttaca cttgcgctag
    20821 catggttcaa cagatgaaag caaacaataa cacggttttt tagacccttg tacaaagatt
    20881 cctacataac aaaatgaggt ggagaaaatg aaagatttaa ggtatagaag tgtctttgat
    20941 attattggac ctgtgatgat tggtccaagt agctctcaca ctgccggagc tgctagaatc
    21001 gggaaaatca tacgtagtat atttggcgaa cagcctgatt ctgtagatat ttatctatat
    21061 gaatcatttg caaaaaccta tcgggggcac ggaacagata ttgcattagt tggcggcctg
    21121 ttagggatgg aacccgatga tgaacgttta gctgattctt tgaaattggc atatgaagcc
    21181 ggtatggaag tcttgtttgt tcctaagagt gaaaaagccg aacatccaaa ctctgtaaag
    21241 atgcttctaa aaaaaggaac acgaaagctt tcagtcactg gcatttcgat cggcggtggc
    21301 aatatccaga tttctgaatt gaatggattc aaaatttctt taagcatggg tactcctacc
    21361 ttgatcgttg tccaccaaga tgttccaggt atgattgcta aagtgactag tatactttct
    21421 gagaccaata cgaatatcgg cactatgaca gtgacacggg aatcaaaagg tgaaaatgcc
    21481 attatgataa ttgaagtaga tgatcctcaa gtagaagaaa cggtaaaaag actcaaacag
    21541 ctgccaaata tcgacagcgt aaattatttc gattaaggag ctaaaaaaat gtttttatcg
    21601 attgaagaat tagtagatca agcaaaagct tatccaagtg tagcagaatt gatgattgca
    21661 gttgaaatgg aaatgagtgg ccgttcccgt agtcacatca tcgagatcat ggaaaaaaac
    21721 ctctcagtaa tggaacaatc aatcaacgag ggaaaagaag gtgttacctc cgtcacagga
    21781 attaccggtg gtgatgcaaa aaaattagat gattatttaa atgaggcaga ctttttaagt
    21841 ggagaaacaa tcttaaaagc tgtacgcaat gctgtagctg ttaatgaagt caatgcaaaa
    21901 atggggttga tttgtgctac accgactgcc ggaagcgcgg gtgttgttcc tggtgtgctg
    21961 atggctgtcc gagatcggct tgatttgaca catgaacagc aattggaatt cttatttacc
    22021 gctggcgctt tcgggttagt catcgcaaac aacgcttcta tcagtggtgc agaaggggga
    22081 tgtcaagcag aagttggttc tgcaagtgca atggcagcag cagctttggt ttgtgcaaaa
    22141 aatgggactc ctcatcaagc agcacaagca gtagccatta cattgaaaaa tatgatggga
    22201 ctgatttgcg atcccgttgc aggcttagtt gaagtcccgt gtgtcaaacg taatgcttta
    22261 ggctcttctc aagcttttat ctctgctgat atggcattag cgggtatcga aagtgtcatt
    22321 ccgccggatg aagtcgttgc tgcaatgtac caagtcggac gtcaaatgcc gtcgattttc
    22381 aaagaaacag cagaaggtgg cttagctgtt acgccaactg ctaaacgact tgcggaagag
    22441 attttaaata agagggcgtg ataaagctcc ttaatttcga gtattaagag attagttatc
    22501 ttcataacaa aaaacgaaga gaggactaat tgcccttctc ttcgtttttt attaatccat
    22561 caatttcgta ttttttactt catcgatcgt ttttgttcct gcaagttgca tcgtgatcga
    22621 caattctttg ttcaggtgtt caaaaacaga tgttactcct tctgccccac ctagattcaa
    22681 cccataaatc actggtcggc caatcgcaac taagtcagca ccacttgcca gagctttgaa
    22741 gacatgctct cctcgacgga caccagagtc gaaaatcact ggtacacgtt tgttgactac
    22801 ttcggcaatt tttggcaata cttcaaagga cgcaggacct ccatctagtt gacgtccacc
    22861 atgattggat acccagatac catctgcccc tgcagagata gcgattactg catcttctgg
    22921 cgattgtatt ccttttacga taactggaag atgggtgatt tctttgattg ttttgatgtc
    22981 atctggtgtc aagccttgtt ttgcagctgc ataaatttct gcgattcctt ttccttcgcc
    23041 attaccactt gcactttgtt cgctatacgc agctaagtta ggcattggta atggaaattg
    23101 gaattgattg atgacgtctt cttcacggta accaccgagc gttgaatctg ccgttaaaat
    23161 gatagcttta gcccctgctt ctactgcttt gttcaaaatg aactcattga atctgtcgtc
    23221 tttactcata tataattgga aaaactgcgg tgcgcctggc gctgcatcgg ctgcgtcttt
    23281 gattgtcgta tttgcataag tgctgatgga aaagattgat ccagcagcag ctactccttt
    23341 agctgtatca gcttcccctt tttcatgtgc caatccttgg gccgctgatg gcgcttggat
    23401 aatcggtgta tctaattcaa ttccgaaaac agatgtatgc aaatcggctg agtcgattcc
    23461 gcgtaatatc cgtggcatga ttttctttgt attaaaagaa gtcgtatttt ctttcatcgt
    23521 ccactcatct tcagaaccac cgcgaatata gccaaaagct cctgcttcca tgcgctcttt
    23581 tacacgacct tctagttagg caatgtttac aatatcgatt ggatgttctt cattacttgt
    23641 atgataagtc atagtagatc cttctttcat cttcttgatt acatataaat catactaaac
    23701 ttacaaaaag taagcaaaca tttactctca gatactttta tcgctattct ttccaaataa
    23761 atcgacttac aagctgatct acttcttggt tttcatgaag catactatga gaggcatttt
    23821 cacctgtcac tactttttcc tctatttcat ttccgcgttg cttcaacaac gcatagaccg
    23881 ctaatgcact gtttaacggt actgttccat cactcaagtc tgtttcatct agttgaccag
    23941 caatcaagaa aaatcgagtg gttataggaa tattaccgat catttgctgg taatcttgat
    24001 agcggttaga aacgacagca ggcccctttg ctaactcatc tgtcagagac tgcgcactat
    24061 catcgacaaa atcattaaaa ggcgcgccaa ttgctgttaa tgtctgaact tgtggcaatg
    24121 acgtatcctg tccatacgtt cctaaatagc gtagtgcact aactcctccc atggaatgcc
    24181 ccactatgtt gactttatct atgccgtagt tttctttcag atacaaaaga caagttttga
    24241 tccattctgc ttgattccac tcgttatttt tattatcttc aaacaatact tggaccatcg
    24301 gattgtctgc ttttccagac aaatctcctg ttgcttgtat ttgtccatct gttcctactg
    24361 ttaaaaccaa ttcttttact gcttgctgat ttttttccaa acgccgcaac atccctttaa
    24421 aggaattttc tgtcccgctg tagccgtgga aaaaaagtgt tggaatagtg gttttttcta
    24481 tcactttact ggattctgcc gttttttctt tatcagaaac ttcagtagta tttatcttcg
    24541 tcgtctggca accacttaac gcaaacaaac tgataaggac taaagctgct gcagttgcac
    24601 gtttgcataa atttttgatt ggttctgtca tacacctctt cctttccata tagaaaaaag
    24661 aaagacttga aaggccaatc atatgatcga catttcaagt ctctttatta atcgatttta
    24721 tctacttgat cgaaatcaag ttctgtactg gtttcacgtc caaacatatc gatgttgact
    24781 ttcaattttt ctttttctcc gtcaacttct gttacttgtc cttctaatcc agcaaatgcg
    24841 ccttcgatga ttttcacgac ttcgccaact tctacttcta gatcattttg tcgcaggctc
    24901 ataccgatag aacgcaagat atgattgact tcttctggaa gtaatggtgc tggtttgctt
    24961 ccggctccgt gtgaaccaac gaatcctgtc acaccaggtg tgttgcggac aacataccat
    25021 gaagcgtctg tcatgatcat ttcaacaagt acgtatcctg ggaatgtttt atgaacgatt
    25081 tctttttctt tcccattttt gacttcagtt tctttttctt caggaacaac gacacggaaa
    25141 ataaagtcgg ccattttcat actttgcgca cgagattcga tatttgcttt tactttgttt
    25201 tcatatcctg aatatgtatg caagacatac cagttttttt caaaagattc cattgaaggc
    25261 tcctcatttc attgtaaaat aaaaaaacct cagtacaccc gaagtttttc tttcgcttca
    25321 ttatagcact aaaagtacgt tgtggctagc gagttatttt aaaatccagc caaataagct
    25381 ttggatggct gtatccatta cgtaaaacat caatccgaaa ataatagaag tttcgatgac
    25441 tactaacgta tctttacgga gttgttttct gcttggccaa gttacttgtt tgatttcatc
    25501 ttttacgctt tttagaaatt tcatgagaca acctcccttg ctactgttta tttcgtttct
    25561 ttatgtaatg tatattgatt acaatattta caaaacttat tgatttccaa acgttcgccg
    25621 ctttttcctt cactgactga ttttgtgtag ttgcgagacc cacaaacgga acaagcgagt
    25681 gctgcttttt tctttgccat ttacttctgc ccccttgctg agaatcagac ttatacttca
    25741 taacagtatc acgtaacttt ttacttgtca atttgtgaaa taaactatct attgttttgg
    25801 ataaaattat gatatgatta atttgaaatt aagctaaaat gagacaattg gaggtatagc
    25861 gatgctatta aaaacacttg tatataagaa aaaagatctg acaaccgtta cagagactag
    25921 tacattagaa gaagcattaa aaattttaga aacttccgga taccgttgtg tgccgatctt
    25981 agattcttca ggtaatattt tccgtggaaa catttacaaa atgcatatct atcgtcataa
    26041 agcaaatggc ggtgatatga cgcttcctgt cactcatctt ttgaaaaacg ctacaaagtt
    26101 catttacctg gatacttctt tcttcaaagt attctttacg atcaaagagc ttccttatat
    26161 tgctgtcctt gacagtgaaa atcattttta tggtattttg acacatagta cgttgcttaa
    26221 tatgctctct caatcttgga gcgtagaaca aggaagctat gtactgacaa ttgcttccac
    26281 aggaaagcaa ggagatttgg ctgctattgc aaaaatcatt tcaaaacaca gcagcattgc
    26341 cagctgtatt actcttgata tcgaaaaaga cgaatacatt cgacgtacat tgatcacgct
    26401 accggctaac actgaagcga aagtatgtga aacgatcgtc aaccacttag agaaaaagaa
    26461 ttataaagtg gtagaaattg aaaatttgca aaaccaagaa gcataatcaa aaagcgaaaa
    26521 aagaaaacag ctaatctgct ttcttttttc gctttttata attagacacg cttttcaaaa
    26581 tcaagcgcgc gatctaaata atacatcaat ggtgctgaaa cagccatgac gataaactca
    26641 cttaatgcaa gcgtgccata ggttgtccaa aatgcttggc cacctgttgg aactaacata
    26701 taagcaatca gtcccatgct gatggtaaag aagatcgtgt tcaatactaa acgcatcttt
    26761 acatttttca ctttgttttg caataaagct gtcaggccta aagaaagcaa tgtttggccg
    26821 ccaccaaaaa ccacatccat gataccaaaa ccaaagaagg cattatagac gatgaccccg
    26881 cctaaaactc cccaaagcaa tttacggtta aagacaacta aatgattcaa actttctgaa
    26941 atacgaaact ggatcggccc tgaggataca ggcgcaacta aaatagttaa cgctagataa
    27001 agagccatga tgatgccatt tacaacgatt acggataacc gttgtctgct tttcgttttt
    27061 tgcattttct ctcctctttt ctcctagttt ttttaacgcg ggatggttga tgaaccgcgg
    27121 ttatgtgaaa caaacaataa ttataacaga cttttcattt atttcaatcc gtcttatctg
    27181 cttttaaaaa agcaaaagct taaacaatgt aaacgtttgc taaaaatcgt gggatatggt
    27241 ataaagtaag tgtaaggggt cataagaatt cccagtaaag cagacgaagt aggatctgca
    27301 agaacagaat tgttgtgaaa cttaaataaa taaaaaagga tgtgggaatt tgagcaacct
    27361 cttactagag aacggtctag caagcagctg aagatctaag tggacatgcc gaaccttgga
    27421 acttctcctt acgtaagtaa tggatcatgc gcttgctagg cttttttgtg tttttcggtc
    27481 ttcggacctt tttcattttc ctagacttct gctataataa ccaagaatta attttgtaac
    27541 agattttttt gaaaaaacaa gtttctttca ttcaaaaaat agaaaaagct tgctaattat
    27601 tggttaacca gcaaacagga gtattaaaat atcttgtttt attgaatagc aggaggaaat
    27661 catgaaattc cattatcata tattgatcaa tccatcggca ggcagcggaa atggctataa
    27721 agtagcagaa agaattttac cggtattaaa aaataagcat attgactata cacttcatta
    27781 tagtgaatac aaaggacacg atgctgagat tgctgaaact ttggcaaaag aaacattgat
    27841 tccttggata gaagaagaac agaaaacaat cgatactttc ccccttttga tcatcgtagg
    27901 aggtgacggc accttacatc aggtacttga tactttctat cagatggaag tagaattccc
    27961 agttgcttat attccagcag gttctggaga tgattttgct cgaggggcag gccttccaaa
    28021 aaatcccaaa aaaggacttc agctgatttt agcagctcaa tcaccagaaa aagtccacat
    28081 aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata gctgttaata atttcggtat
    28141 aggattagat gcagctatcg tccatgcgac aaatcattct tccacaaaaa agcgattgaa
    28201 caaatacaac ttaggatcat tttcgtattt attctctatt ctccgtgctt tattcactca
    28261 aaaaggattt ccaattctgg tagatgcagg aggaaaacaa ttaagttttt caaacgcatt
    28321 tttatgtaca gcaacgaagc atcctttttt tggcggagga atcgcgattg ttccaactgc
    28381 tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa cgaatcaatt tctttaaaat
    28441 cttgtggctc attcttttac ttatacagaa aaaacaattg aaatcgaaat atttccatca
    28501 ctacacaaca aacagattac gtatcgtttc aacaacaccg caacatggtc aagaagatgg
    28561 agaagaaatg gaaaaacgac cttttgatat cacaataacc acaactaaac agttgttttg
    28621 gtgttaataa aagaggtttt gtcagaagct acatctggaa caccgtttat tcggaaaaaa
    28681 ggactgtgac acgacttttg tcacagtcct ttttttacta tgcagttaat tctttgccca
    28741 attgggttgc catcgatagt ttctttcttt acccaaaata aattggatca ttgaaaacac
    28801 cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa gattctgcaa atgtttgatt
    28861 gtaactgagt gtttcactga tcaagttatt attttgaaca aaccaatatg ttggtgtgaa
    28921 tgccgctatt ttgctaacca tttctggcaa gtattctgct ggtacaaaca ccccaccgat
    28981 aaagcaactg cctgtgataa aaatatcttt gatcccaccg ctggtttctg agttttttac
    29041 caatgaagtg atacaagccg aaaagctaat gatcggcaat aaaaacaaga aagtatttaa
    29101 aatatgatac cataccgtta tatcccacga ggtgtggatc actaacagaa ccattagaag
    29161 taagaagcca ctgaacaaca gcagcgagta actaatcaaa ctaaaggtta ctttgcgatt
    29221 caagtttcgt ttggtaatgg gcgcactctg gtttcgttta ctgatctctt tgcgattaaa
    29281 tgctagattg atgacgctga aaccgctaaa aatggataga aacaacccat aagacaagaa
    29341 attaaaggtt gtgcccgtca acagcttttt catccgctga ctatatcccg cacttagctc
    29401 aacttctcca gtaaccgaga gtgccatttg tgtctgttcc atagctgcat cgattgtttt
    29461 atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg ttcacttgct gtgtaacaac
    29521 tgttttagaa aaggcatctg ggcgactctg tatcttgatc gtcggcatct ttcctacttc
    29581 gacttgttcg gtaaagtctg caggcagatg taagataatg tttacacgat taaaataaag
    29641 agcatcatcg atttcttttt gtgaggttcc atccaatgta acgactgttt ccttgtcatt
    29701 caaatattta cgaaggccat cgactacagg cgactcttct tcactaatca aggcgatttt
    29761 cgcatcttgc agagaggtat tttcaacatc aatattttgc gcaaaaaaga atgtggcaac
    29821 aacagtgata attacaccta tcagtaaatt cgatttattt gctttaagca atgtccaaat
    29881 cgttttataa agcgtcataa cgcacccctc tttccatcca agcatttcct aacgcaaaaa
    29941 ggaaaataaa tccgatcaac catagaagat tggtataaaa actagccaaa tcagtgtaga
    30001 aataaagttt
//
LOCUS       DARDHU010000022        29713 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000022 DARDHU010000000
VERSION     DARDHU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.022"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..442
                     /locus_tag="SDD03_001058"
     CDS             <1..442
                     /locus_tag="SDD03_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG4303523.1"
                     /translation="QNVVALFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMI
                     QKIQLGCAWWFNDTAEGMRQQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLC
                     QLLSEWVKAGRIPDDEMYLSQIVENICYRNAYNYFNFFEIESRK"
     gene            526..1173
                     /locus_tag="SDD03_001059"
     CDS             526..1173
                     /locus_tag="SDD03_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG4303524.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            1302..2678
                     /locus_tag="SDD03_001060"
     CDS             1302..2678
                     /locus_tag="SDD03_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4303525.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            2689..3483
                     /locus_tag="SDD03_001061"
     CDS             2689..3483
                     /locus_tag="SDD03_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4303526.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            3496..5262
                     /locus_tag="SDD03_001062"
     CDS             3496..5262
                     /locus_tag="SDD03_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG4303527.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            5290..7098
                     /gene="uidA"
                     /locus_tag="SDD03_001063"
     CDS             5290..7098
                     /gene="uidA"
                     /locus_tag="SDD03_001063"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG4303528.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            7270..7671
                     /locus_tag="SDD03_001064"
     CDS             7270..7671
                     /locus_tag="SDD03_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4303529.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            7665..7910
                     /locus_tag="SDD03_001065"
     CDS             7665..7910
                     /locus_tag="SDD03_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303530.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(8234..8455)
                     /locus_tag="SDD03_001066"
     CDS             complement(8234..8455)
                     /locus_tag="SDD03_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303531.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(8617..8689)
                     /locus_tag="SDD03_001067"
     tRNA            complement(8617..8689)
                     /locus_tag="SDD03_001067"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8654..8656),aa:Lys,seq:ctt)
     gene            8765..8995
                     /locus_tag="SDD03_001068"
     CDS             8765..8995
                     /locus_tag="SDD03_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303532.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            9039..10208
                     /locus_tag="SDD03_001069"
     CDS             9039..10208
                     /locus_tag="SDD03_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4303533.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            10782..11351
                     /locus_tag="SDD03_001070"
     CDS             10782..11351
                     /locus_tag="SDD03_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG4303534.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            11338..12198
                     /locus_tag="SDD03_001071"
     CDS             11338..12198
                     /locus_tag="SDD03_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4303535.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            12388..13092
                     /locus_tag="SDD03_001072"
     CDS             12388..13092
                     /locus_tag="SDD03_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4303536.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            13097..14932
                     /gene="walK"
                     /locus_tag="SDD03_001073"
     CDS             13097..14932
                     /gene="walK"
                     /locus_tag="SDD03_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG4303537.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            14929..16245
                     /locus_tag="SDD03_001074"
     CDS             14929..16245
                     /locus_tag="SDD03_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303538.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            16246..17115
                     /locus_tag="SDD03_001075"
     CDS             16246..17115
                     /locus_tag="SDD03_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG4303539.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            17170..17979
                     /locus_tag="SDD03_001076"
     CDS             17170..17979
                     /locus_tag="SDD03_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4303540.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(18082..18711)
                     /locus_tag="SDD03_001077"
     CDS             complement(18082..18711)
                     /locus_tag="SDD03_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG4303541.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            18881..20173
                     /locus_tag="SDD03_001078"
     CDS             18881..20173
                     /locus_tag="SDD03_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4303542.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(20249..20911)
                     /locus_tag="SDD03_001079"
     CDS             complement(20249..20911)
                     /locus_tag="SDD03_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4303543.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            21136..21420
                     /gene="groES"
                     /locus_tag="SDD03_001080"
     CDS             21136..21420
                     /gene="groES"
                     /locus_tag="SDD03_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG4303544.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            21471..23096
                     /gene="groL"
                     /locus_tag="SDD03_001081"
     CDS             21471..23096
                     /gene="groL"
                     /locus_tag="SDD03_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG4303545.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(23185..24333)
                     /locus_tag="SDD03_001082"
     CDS             complement(23185..24333)
                     /locus_tag="SDD03_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4303546.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(24435..25019)
                     /locus_tag="SDD03_001083"
     CDS             complement(24435..25019)
                     /locus_tag="SDD03_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG4303547.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(25146..25577)
                     /locus_tag="SDD03_001084"
     CDS             complement(25146..25577)
                     /locus_tag="SDD03_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG4303548.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(25595..25915)
                     /locus_tag="SDD03_001085"
     CDS             complement(25595..25915)
                     /locus_tag="SDD03_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4303549.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            26215..26355
                     /locus_tag="SDD03_001086"
     CDS             26215..26355
                     /locus_tag="SDD03_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303550.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            26653..26910
                     /locus_tag="SDD03_001087"
     CDS             26653..26910
                     /locus_tag="SDD03_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG4303551.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(26881..27189)
                     /locus_tag="SDD03_001088"
     CDS             complement(26881..27189)
                     /locus_tag="SDD03_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303552.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            27267..28013
                     /locus_tag="SDD03_001089"
     CDS             27267..28013
                     /locus_tag="SDD03_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG4303553.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            28028..28231
                     /locus_tag="SDD03_001090"
     CDS             28028..28231
                     /locus_tag="SDD03_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303554.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            28336..28635
                     /locus_tag="SDD03_001091"
     CDS             28336..28635
                     /locus_tag="SDD03_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303555.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            28635..28817
                     /locus_tag="SDD03_001092"
     CDS             28635..28817
                     /locus_tag="SDD03_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303556.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(28823..29299)
                     /locus_tag="SDD03_001093"
     CDS             complement(28823..29299)
                     /locus_tag="SDD03_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303557.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            29352..>29713
                     /locus_tag="SDD03_001094"
     CDS             29352..>29713
                     /locus_tag="SDD03_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEG4303558.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIE"
BASE COUNT        10238 a         4611 c         5885 g         8979 t
ORIGIN      
        1 acaaaatgtt gtagcattgt tcgacactgc tgaaaagagt gggtgcttac caaaaacaat
       61 tttatattca ttaaatccaa ccgattggct tccactagca acagcaatgc aaagcttcca
      121 agggggtatg attcaaaaaa tccaattggg atgtgcttgg tggttcaatg atacagctga
      181 aggaatgcgt caacagttga ctacgatggc gcaacaaagt ttgttgtcga attttacagg
      241 tatgttgact gattctcgaa gttttctaag ctatcctcgc catgagtatt tccgtcgagt
      301 attatgtcaa ttactaagcg agtgggttaa agcaggtcga attccagatg atgaaatgta
      361 tcttagtcag atagtggaga atatttgtta tcgtaatgcg tataattatt ttaatttttt
      421 tgaaatcgaa agtagaaaat aatacaaaaa ataaaagctt aaatttattt attgtaagtt
      481 agtacaacta gctagagtat gtatccataa aagaaggaga tagatatgaa aaaagtagaa
      541 attttaagta agttagaagc ggctggagtc attgcggttg tacgtggaaa aaataaagat
      601 gaggccatta aagcaataaa tgcaattact gcgggtggaa ttaaagggat tgaactaact
      661 tttacagttc cgaatgctga agaaataatc aaagaaattg ctaaaaaata tgctgatcaa
      721 aaagatgtaa taattggagc cggtacagtt ttagatgcgt ttactgctcg tattgcaatt
      781 atggctggag cagaatatat cgtaagtcca tcttttgatc gacaaacagc agagatttgt
      841 aatttatatc aaattcctta tttaccaggt tgtatgacaa ttactgagat aaaggaggca
      901 cttaccgctg gagtagaaat cattaaacta tttccaggtt ctgcttttgg tccatctgtt
      961 gtaaaaacgt atcaagctcc attaccatat gtaaatataa tgcctactgg cggagtaagt
     1021 ctagataact tagaagagtg gtttgaagca ggcgttgttg cagttggtgt aggtgggaat
     1081 ttgttggctc ctgttgaaaa aggtgatttt gaagaagtaa ctcgaatagc tagtaaatat
     1141 atagataagt tttatgagat aaagaattat taggagaaaa aataaataat actataaaat
     1201 gtaaaaaata aatactaact ctttatactt cagagtatag tacgtaagtt ggcagttcaa
     1261 cagaatgaaa acgcttaaca ataggaagga gcgcaaatta tatggaaaga aaatttggtc
     1321 taagagacaa aataggatat atgtttggag attttgggaa tgatttcact ttcatttttg
     1381 ctagttcttt tttaatgatt ttttatacaa aagtattggg gataagcgga gctaccgtag
     1441 gaacattatt tttagtagca agatttattg atgcagttac agacataaca atggggcgta
     1501 ttgtggatgg atcaaaacca tctaaaactg gcaaatttaa accgtggatt ttacggatga
     1561 gtggtccagt cgctttagca agttttttaa tgtatcaaac atcaatggca tcagcttcta
     1621 tgagtattaa agttatatat atgtatgtaa catatatact atggggaagt attttttata
     1681 catctattaa tattccttac ggttcaatgg catcagctat cacttctgtt gcagaagaaa
     1741 ggacagcgtt atcaacattt cgtagtatag gagctacctt agcaggtatg ttcattggcg
     1801 tgttaacacc aatttttatt tatacaagag atagttctgg agctcaaatt gtacgtggtg
     1861 gatcaacttt tacaattgtg gcaggagtat tttcagtgtg tgcagttatt tgttacgtaa
     1921 tatgttataa attaactgta gaacgggtta aaagtgatga atcgcagtta aaagtttcat
     1981 tgttgaaatc tttaaaagga ttagctaaaa atagagcgtt gctttctata atagctgcag
     2041 ctattttttt gttgcttgct cagcttttaa caatgagtat gaacaattat gtatttccag
     2101 attattataa taatgcacag ggaatagttt taatgaactt tatcaatcct attttgatgt
     2161 tggtacttgt ttcacctgta agtttaattt tcagtaaaaa atacggtaaa aaagaattgg
     2221 cgtcagtagg gatgttcttc tctgctattg tatatttact tttatttttg ataaagccta
     2281 gtaatatgta tgtattctta gttcttactt ctgttggata tatggggtta ggtattttta
     2341 atacagtcat ttgggcgaat attactgatg taatcgatga tcaagaagtg aaatctggcc
     2401 aaagagaaga tggaactatt tatgcagtat attcttttgc ccgaaaaatt ggacaagctt
     2461 tagctggcgg tgtcggaggt tggactcttt ctataatagg gtatgattcg ttagcaaaag
     2521 tacaaagtgg agaagtttta caaaagctgt ataactcttc aacgttgatt ccagcagttt
     2581 gtttctttgt tgtaggattg attttatttt tcgtatatcc attatctaaa gaaaaagtat
     2641 tagaaaatta taatttgcta aatcaaaaac ataactagga gaaaaaacat gaacagttgg
     2701 atattttgta ttgattcaga tggttgtgtc atggatacaa tgacttataa acacaatctc
     2761 tttttcgggc caattgctgc agagattttt gatgttcatc atagggaaaa attcctacag
     2821 gattggaata atattaattt atattcagag actcgtggaa taaacagatt tttaggttta
     2881 gttaaaacat tagaaaaaaa tgatgtacca tcgattaatc gattaaagca ctgggtatct
     2941 gtaacaaaag aattgtctaa tccatctttg aagagggaaa tagaatactc tcagtcagag
     3001 gatttattaa aagctttaaa atggtcggaa gaagtcaatc gacagattag tcaagctaaa
     3061 ggattagata aacccttttt aagtgctaga gatactataa aagctctgaa aaaatatggg
     3121 aagataatta ttgtaagctc agctaataag gaagctattc aagaagaatg ggaaagacat
     3181 gaattattat ccttagttga tgaactgtgt tgtcaagata aaggaaaaaa agaagacatt
     3241 attcgaagtg taattgaaaa tggttgtgat ttggataaga tattaatgat tggtgattct
     3301 cctggtgatc tagaagctgc aaataagaat aaagttttct tttatcctat tttggttaat
     3361 aaagaaaaag aatcatggga aaacttaaga actgatattt tatttaaatg tttatcggga
     3421 gagtatatag atattgaaca aaaatatata gatacttttt ggaaaaatct tactaataag
     3481 tagaggagaa agaaaatgac tataaaagta gacttacgaa aaaaaccata tcatttagat
     3541 gaagatgcta tttcttggat tgaagagacg attgatgcta tgactttaga agaaaaaata
     3601 ggtcaattat ttgttaatat gggatctagt cgtgataaaa attacttaag tagtatggtc
     3661 aatcagtacc atattggagc tgtacgatat aatccaggaa ctgctgatga ggtatatgat
     3721 caaaactata tcctacaaac agaaagcaaa attcctttat taattgctgc taatacagaa
     3781 gcaggtggta acggagcttg tacagatggt accgaagttg gtgtagaagt aaaaattggt
     3841 gcaacaaatg atgcgaaata tgcttatgaa ttagggagag tttctggaat tgaagcatcg
     3901 gctattggat gtaactggag ttttgctcct attgtagata ttaatagaaa ttggagaaat
     3961 cctatcattt ctagtcgatc ttttggacaa agccctgata aagttttaga acttagttta
     4021 gcttatatga aaggtattca agagagtgga attttgcctg cagctaaaca tttccctggt
     4081 gacggtattg acgaacgaga tcaacatcta tcttttagta ttaatagttt aagtacagaa
     4141 gaatgggata attcttttgg aaaagtatac tcaggcttaa tagaagctgg attaccttca
     4201 attatggcag gtcatatcca tatgccagct tattcaaaac attttaatct tgatctgaaa
     4261 gatgaagatt tacttcctgc aactttatca aaagaattaa tcactgactt acttagagga
     4321 aaactcgggt ttaatggatt agtagttact gatgctagtc atatgttagg aatgactggg
     4381 gcaatgaaac ggtcagaatt attgcccact tctattgcgg ctggatgtga tctattccta
     4441 ttttttaatg atccagatga agactttaat tatatgatgg aaggttatta caaaggaatt
     4501 atttcagaag agcgtttacg tgatgcatta agaagaattt taggcttaaa ggctaaacta
     4561 ggtttgcata aaaaagaaaa gaataaaatt ttgttaccga aacaagaagc tatggaaaaa
     4621 attggtttaa ttgaaaataa atcaattttt aaagaggtag ctgatcaagc aattacacta
     4681 gttaaagata aacaagatat ttggcctatt gtaccagaaa aatataaaag gattttactg
     4741 gtagatgtac atggggtaaa aggtgggttt ggtgaagtag ttgctggaaa tagacctcag
     4801 gccattgatt tattgaaaca gattttgtca gcaaaaggat ttgaagttga aaaatacatt
     4861 tcacctgaag aagaattgaa aggattatcg caagaagaaa aaattaaaaa tatagctgga
     4921 atatatgctg ctaagagacc aatcaaagat ttaactgaaa aatatgactt gattattaat
     4981 atagctaatg ttaacccagg tacagttcaa agaattgttt ggccagcaag taaaggtact
     5041 ccggatattc cattttatat tcatgaaatt ccaacaattt ttgtatctgt acagtgtccg
     5101 tttcatttag ctgatgtgcc tcaagtaaaa acttatatca atacttatga tggaaaacaa
     5161 gaaactttag aaatattagt ggacaaacta atgggccttt ctgaatttaa gggaataagt
     5221 ccaatagatg cttattgtgg gttaaaggat acatttattt aatatttaaa ttatatagga
     5281 gtgatttaca tgttatatcc cattatgaca gagacgaggc aagtaattga tttaagcggt
     5341 ctttggagat ttaagttaga tacaaatgat gacttacaag aagaacttgc tttagaacca
     5401 atggttgaaa aagagagtta tccaatttca gttccttctt cttataatga tttatttgaa
     5461 acagagaaca ttagaaatca tgttggatgg gtgtggtatg aaaaagaagt gattcttcct
     5521 aaaaaattat tagaagaaag attagttctt cgttttggat ctgcaacaca tgaagcaaaa
     5581 gtatttttga atggaaaatt gattaatcaa cacaaaggag ggtttacgcc atttgaatca
     5641 gaaattaatc agtttataaa agatggaagt aacagaatta cagtggctgt aaacaatgtt
     5701 attgacgata gcactttacc tgtagggatt ttgaaagaaa cgatacagga agatggagac
     5761 ataaaaaaag aaaatttggt taattttgac tttttcaatt atgcaggcat taatagaccc
     5821 gttaaattgt atacaactcc gaaagactat ataaaggatg tcactattaa tacaaaaata
     5881 attgattcag atgcaaatgt agaatttaat atcgatacag aatggaccgg agaaattcta
     5941 atagcaatct atgacgaaga agctcaactt atagcagaag ctaaggggaa agaaggagtg
     6001 attcaactta agaacccaac actttgggag cctctaaatg cttacttata tagtatgaaa
     6061 gttagtttaa ttaacgaaaa taaagtgatt gatacgtaca cagaagaatt tggcattcgt
     6121 agtattcagg tttcgaatgg gaagtttttg attaataata aaccttttta ctttaaggga
     6181 tttgggaagc atgaggacac gtatattaat ggtcgtggtt tgaatgaagc agtaaatatc
     6241 aaagatttta atttaatgaa atggatcggt gctaattcat ttaggacttc acattatcct
     6301 tattcagaag aaattatgcg cttagcagat cgagaaggta ttgtagttat tgatgaagta
     6361 cctgcagtag gattacattt gaatttcatg gctactttat tagatgacaa cgtagaaaaa
     6421 catgatactt ggaaagaaat acggacagaa aatgctcaca aaatggcgat taaggaattg
     6481 atagaacgcg acaagaacca tccgagtgtc gtaatgtggt ctattgcaaa tgaaccagat
     6541 tcggattctg aaggcgctaa agaatatttt gaacctttag ttgaattgac taaagaactt
     6601 gaccctcaga agagacctgt aacaatcgtt acttacttaa agtcaacacc agatatttgt
     6661 aaagtaggag atattgtcga tgttttatgt ttaaatcgct attatggttg gtatgtttca
     6721 ggtggagacc taaaacatgc aaaatatctg ttgaaaaaag agctagatgg atggatggaa
     6781 agatgtcccg atacgccaat aatgtttaca gaatatggag cagatacagt agctggactt
     6841 catgatacag taccaacaat gtttacagaa gagtatcaag tgggttatta cgaagcaaat
     6901 cacgaagtaa tagataaatg tgacaatttt gttggtgaac aagtgtggaa ttttgcagat
     6961 tttgaaacaa gtcaagggat tttaagagtg caaggaaata agaaaggaat ctttactaga
     7021 gatagaaagc ctaaaatgat tgctcactat ctaaagaaac gatggtcatc aataccaaat
     7081 ttcagttata aaaaataatt taccttttat aaggaatata aaaaaggagt ggctgcttaa
     7141 ttgccaactc ctttttttta taaaaagatt ttctgtttaa agaaggatat ttttttctag
     7201 ttttttcgaa aaaaaggcgc tttctgattg ttgtattagt ggaaagggga aaaaaggagc
     7261 atatttatca tgcaacaaaa ttacaatgag aaatctatac aacatcaatt tgatgcctac
     7321 tgtaaaaaag tattacgaaa cgaaactaag tctattcgaa aacgaaacat taacataaca
     7381 gataatgagg aaccattgac gaattcaaat gaaggtaagt actcccaaac tcattttgat
     7441 gaacaagatg tctatttctt atttggaatg gaaatattaa tttcagatca aaagttatcg
     7501 gcagccattg atcatttatc ggaaataaga agaaaggtta tcctacttta ttattttgca
     7561 ggttttaatg atactgaaat cggtaaaatc ctcaatatga gtacaagtgg tatctggtat
     7621 caacgaaaga aggcagttga acaattaaaa atggggtatg acttatggta gacaacccca
     7681 aaaaacggca gtatccatta gttcctctat ccattatcca atctgctcta gaagaagatc
     7741 caatttcaat caagatgata ctcaaacatt atcatagtta tatagctaag ttatgtttga
     7801 caaatggatt taatgaggcg gggcaattta ttacctatgt agatgagtat atgctaagac
     7861 agctagaaat taaattgata gaagcgatct taaaatttaa aattaactaa ctaaaaagtc
     7921 ccctttccac ttattctgtt taaaagaatc ttctaatagt taaaggatta gtagattctt
     7981 ttaaacagaa aacctattga tcattgaaat ttaaatacat tctatcagta cgttaacact
     8041 tcaaggagca tattttagtt gcgccatgat ttcttactgt aaagaaggag cgataaacat
     8101 tctaaaataa tttttatagt caataaaaag cgatgatttg aagttgggat aatgatactt
     8161 ccgtaaggct cgccgtaaac ttggagagag gtgaaattcc tatgtgcttt tgacaaaagc
     8221 agctgattaa gaattatttc catatgttga tttcaacatt ttttctatta tttttaagac
     8281 caaattcagt gaggcaaata gcatcatgta aatttagttt ttctattgaa cgttctccac
     8341 tttttagttt ttttatagta ctcaaattta atcctgtatg tttagagatc tcatttgcag
     8401 tatggttttg taaagtttct cggattattg ttatattagc aatttcatta cgcataatta
     8461 tgttcctttc ttatcaaaaa tatcaatgac tacagtgggc ttttagtgaa aaaaaggggt
     8521 actacagtct cttttgaaaa caaaaaaata cagtatatac gttttctaaa tgctaagcac
     8581 caacgtttac actgtattta agggtttggc acccaatggg ctgtgagggg ctcgaacccg
     8641 cgacccgatg attaagagtc atctgctcta ccaactgagc taacagccca aattaatact
     8701 ttgttattct aacacctaaa ataaaaaagt gcaatactta ttatcagaat tgagagtgaa
     8761 ttttatggat tttcaagatt atgaaaaaat atttgaaagt tatccatcga tattgaaaat
     8821 tactgattta actaaaatgt taaatattgg aaaaaataaa gcttataatt tgctgaaaga
     8881 tagatcaata gaaaatataa aaattggagc aaggtacaaa atacctaaaa aagctgtcat
     8941 tgagtatata ttgaaaatac aagatgaagg taaaaatcat gctataataa aatagtcaaa
     9001 atttttgccg atatttcagt ttaagaggag agattgaatt gaaattaaga gcaaacataa
     9061 ttgaaaaaag aggaacatta caagtagttg tatcttataa agactctaac ggaaaaatta
     9121 gacaaaagtg gaaagataca aaattaaaaa agaaaggaaa taaaaaacga gcagaaaaaa
     9181 ttagagatga atttttacta gaattggaaa gtcaattagc tatggaatgt gaatgtgcta
     9241 atccaaattt attgttttat gactatttgc ttaattattt ggaagtatca aaaaaacaag
     9301 tagcatttaa cacatatgtt gggtataagc attacgtcca taatcgaatt tacaagtttt
     9361 tttatcctaa aaaaattaaa ctaaataggt taaagcctta tcatttacaa gagttttatc
     9421 aatatatgtt aaatgataac tgtacagcaa atacggttat tcattatcac gcatttatac
     9481 gcaaagctct tcaagaggct gttattacag aattgataag tacaaatgta gcagataaag
     9541 taagaaagcc aaaaaagaaa caatttgttt ctcaagttta taatcaacaa gagatcttga
     9601 aattacttga cattttgtca gaagaaaaac tctatttggt agttctttta acagcatttt
     9661 atggattgcg aagaagtgaa gttttaggat taaaatggtc tgctattgat tttttaaaca
     9721 aaaaaatcat gataaaccat gtaatcatag aaaatccaga gaatatgtca cagttaataa
     9781 agaaagatca aacaaagaac tcttccagct atcgtacatt acctttggtt gaaacgattg
     9841 aacgagcatt agtaaatcaa gctaaatggc aacaagataa tcgtattctt ttaggagatg
     9901 attatatttt agaagataat gagtacgtat ttactatgga agatggacgg ttgatgaagc
     9961 ctcaatatgt aacgcatcgt ttgagtaagt tgattaagaa aaatggattg aaaaaaattc
    10021 gctttcatga cttgcgtcat tctaatgctt caataatgtt agatagtggc cagaatatga
    10081 aatccattca agaatggctg ggacatgcat cgtattcgac aactgctaat ttatacacgc
    10141 atttaaccgc aggagtaaaa gatcgtgcag cagaagcatt agaaaacgca tttgggttta
    10201 aagaataaaa aaacaaaaaa caatatcttt ggggtatcgc ttcttgtttt tagaattttt
    10261 ttgttggcaa tttgttggca gtatgttggc aatgaaaaaa ttcacaaact aaaaacctat
    10321 ttataaagct ttataaaact tttttcacaa atagagcatt tggttaagag tcagctgctc
    10381 taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac taagaatttt
    10441 aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta taggaaatca
    10501 ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac aaagttcttg
    10561 caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg tctccccggc
    10621 gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt ttttgttttc
    10681 atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt ttatcataag
    10741 tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa atgcgtcagc
    10801 aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg gaagaactcc
    10861 ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat ctttcacctg
    10921 tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat tacgttaaac
    10981 taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta gcatttttac
    11041 ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa gggatattgt
    11101 tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat gaacaagaag
    11161 cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa aaattcgacc
    11221 aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt gcaatgaaac
    11281 ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag aaaacaaatg
    11341 aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgttccaga agaattgata
    11401 aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca ggtatataca
    11461 gataaaattg acattacccc tgaagagatc tatcaacgtt tgccagagga aatccctggt
    11521 acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa aaaagacggt
    11581 tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata taatgtggtt
    11641 cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac gaaaaatatt
    11701 ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga aggagtagaa
    11761 tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt tttctttaat
    11821 gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc ttctattgta
    11881 ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat ttattacaca
    11941 gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta cgtcaaagaa
    12001 aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc gaaagaagaa
    12061 gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca aatctacttt
    12121 ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg tgtcggtgta
    12181 caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca gtgttttcct
    12241 tttttattga agttagaaca aaaaataata aagtattttg cactttcatt ttaattttct
    12301 cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg agaaggtatg
    12361 acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga tgatgagaag
    12421 ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgacgt ttatactgca
    12481 tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt gattttactt
    12541 gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg caaaacttat
    12601 gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt cctaggctta
    12661 gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt agttgcacgt
    12721 gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga ggcagcacct
    12781 gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt gacgaaacga
    12841 ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt ggcgaagcac
    12901 cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta tgattatttt
    12961 ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat cgaagataat
    13021 ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt gcgtaaccca
    13081 gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca attttaagat
    13141 cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg cctactttat
    13201 acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc aaacggtgga
    13261 atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag atgatgaaga
    13321 ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata tcatcgaaat
    13381 acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg aacaaagcgc
    13441 tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac gttatgttgc
    13501 tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt ccccgaccgg
    13561 cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat attcggaaat
    13621 caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta tttcaatgat
    13681 cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga gagaacaagc
    13741 cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac aagatgaact
    13801 tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag ctcaagatac
    13861 aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag acggcgtcat
    13921 tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt ccttgctaga
    13981 tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata tcgaagaaga
    14041 atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc gttcaaccag
    14101 tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc gtcgcgaatc
    14161 aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag aaaaaacaga
    14221 acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac cgttgacaag
    14281 tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg aagtagcacc
    14341 aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga tcaatgacct
    14401 cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat atgtcaattt
    14461 caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa atcaggaaaa
    14521 aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag tagatacaga
    14581 taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt caccagatgg
    14641 gggaacgatt acttgtcgct tattagaaac gcataataac gtgattttaa gtatcaccga
    14701 tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt tctatcgagt
    14761 agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta tttctagaga
    14821 agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa aagggtcgac
    14881 tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat gagaatctct
    14941 gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata cttctcttat
    15001 gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac aaacactcaa
    15061 atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct tcatgttaca
    15121 tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta ccgattgcaa
    15181 ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg agacaaatca
    15241 cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa tgcgcctttt
    15301 ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa cttggcaaaa
    15361 gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa aaaaatccga
    15421 tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa ttggacagat
    15481 atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac agaaccatcg
    15541 acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaagaagta tagttatatc
    15601 ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc agatagcgtg
    15661 aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac aatgtctatc
    15721 cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa agaaaatgct
    15781 agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt agggaacctc
    15841 agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga aggcttcccg
    15901 gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga aacgaatggt
    15961 tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat accttcagac
    16021 gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc taagggagca
    16081 gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt caaagaaacg
    16141 aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa ctggtattca
    16201 gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga tttcaaaaaa
    16261 atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt cagcagttat
    16321 catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga atcaatccaa
    16381 caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga gaacaagcaa
    16441 ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa ttatcgcaag
    16501 gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt tttgacgaaa
    16561 tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat tagtagttcc
    16621 ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta tgtgtacacg
    16681 aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca agagtatgaa
    16741 ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa aaacgataat
    16801 gattatagca tcacgaaata catgcagacg catttatctg atatcgagca gctgcgcgaa
    16861 aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa taacaaaata
    16921 ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct gaaagtccgg
    16981 gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga taaagtggtc
    17041 caaattgaga gcgtcaacgc tttcagcaat acgatcgtga caaacaatac tgtaccaaaa
    17101 gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa gaagggaagt
    17161 aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc aagtggaagt
    17221 tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga tgcaggactt
    17281 agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga ccaattagat
    17341 ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt cttggctcgt
    17401 aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga accactgatt
    17461 ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag aacattcggc
    17521 gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca attttatcgt
    17581 ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg cagtgattat
    17641 atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca tgatcttgag
    17701 atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg agatagagga
    17761 catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa tcggaccaaa
    17821 cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc tcatttaaca
    17881 atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga aatctatgat
    17941 acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc aataaaaata
    18001 taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt atttttttag
    18061 catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct tttcatgttt
    18121 ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga tcgtactgtc
    18181 gctagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg tacgtttgcc
    18241 atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt tagattgttt
    18301 caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg catttttagg
    18361 tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag aggcaccccc
    18421 aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg acaaagaagt
    18481 ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa catcaaaaga
    18541 tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa catctgatgg
    18601 caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca atgcttcgtc
    18661 taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca taaaaaacct
    18721 ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga atccgatttt
    18781 tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag ttaaattaag
    18841 aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt ggttagatcg
    18901 tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg ctttgcgtaa
    18961 tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct tatttgctca
    19021 ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta tgtattttac
    19081 acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg ccattgcata
    19141 cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag ccttgtctgc
    19201 ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga caggtgctga
    19261 agggatacta ggagcagtta caactgggct catcatcgga tggggctatg cacgtttagt
    19321 aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt ggcagtcttt
    19381 tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt cagcggtttt
    19441 tcaacatttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc agcttcccat
    19501 tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg ttccgttttt
    19561 atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc ccgtcttgca
    19621 agcgaattca ttagcaaatg cagaaatctt agcctcagga caagagttga cggtggcaaa
    19681 tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta cgggtgctgg
    19741 attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga aatacagaga
    19801 attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa ttattttttc
    19861 cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac cggtcgtttc
    19921 aggattgatt acctattccg ctttatattt tggattcgtt cctttattta cagcagtcca
    19981 agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag tgcctgcagc
    20041 gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat ttttgaaaaa
    20101 aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag cagcagaaaa
    20161 gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa tcaccaatct
    20221 aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca gctaattgaa
    20281 caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag atccgaccag
    20341 ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc agatgaccat
    20401 cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa aaatttgaat
    20461 atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg atgggtccag
    20521 caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg atattttgtg
    20581 tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca gcgatgcttt
    20641 gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt ggagcagaag
    20701 ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa aaaatagcta
    20761 ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca gacgtagatt
    20821 ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca tagcaaaaaa
    20881 tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg gataacaagc
    20941 atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat aagcagctgt
    21001 cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag aatgagtatt
    21061 attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa aaaagagtta
    21121 tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga agtcgcaaaa
    21181 gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga gaaaccccaa
    21241 actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga aaaagttcct
    21301 gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac agaagtaaaa
    21361 tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat tgttgagtaa
    21421 atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc atggcaaaag
    21481 aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat aaattagcag
    21541 atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa aaatcttacg
    21601 gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa ttagaagatc
    21661 attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca aatgatattg
    21721 caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc gaaggtttaa
    21781 aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa ttggcaacaa
    21841 aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa gaagccattg
    21901 cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt gctgatgcaa
    21961 tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg atcgaaacag
    22021 aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa tatatggtaa
    22081 cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc acagacaaaa
    22141 aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa caatcacgtc
    22201 cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt gtattgaaca
    22261 aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc gatcgtcgta
    22321 aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact gacgatctag
    22381 gtttagaatt gaaagatgca acaatcgaaa acttaggaaa tgcttctaaa gttgttgtag
    22441 ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc gaagctcgtg
    22501 tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc gaaaaattac
    22561 aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt gcagctactg
    22621 aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct acacgtgccg
    22681 cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt atcagcaaag
    22741 tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt gttcgtgctt
    22801 tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca gtgattgttg
    22861 ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga gaatgggtaa
    22921 acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc ttgcaaaatg
    22981 ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat aaaccagaac
    23041 cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg atgtagtaat
    23101 taagaaagaa ccctatttta acagggttct ttcttctttt tttgttttat tttaagcaaa
    23161 aggggcaaag aaggggcaaa aatatcaaag gctatttagt ttatcaatta cttgttgttt
    23221 ggctcttttt gttacatggt tataaataga cagagttgtg tttgcatcag aatgccctac
    23281 acgctccata atggctttaa gaggtacgcc taattcagat aatagtgaaa cgtggctatg
    23341 tctgaatata tgtgaggata gacttttttc taattccaac tcttcctcta ctttatggag
    23401 tatagcgtta aatgagtgca gcgcaagcgg agtgccactt gtagatataa atatatattg
    23461 atcgggatct gtgggtctac ctgcaagaat attgtcagct atcacacttt caatcaattc
    23521 ttttgcacga ttgggtaatt gcacttcacg ttgcgaataa gtattttttg gagttgtttt
    23581 tatagcatta tccattttca cagatgtgta atctaaggtc ccattaatgg aaattttccc
    23641 atcctcatag tccttcattt gcaaagctag caattcccca tatctcaaac cagttaaata
    23701 tagaaactca gctattatgc cgtgtagttt tctgcgagga ttggagtata gctgtttcag
    23761 tatttgatca atttcttctt tctccagata ttttttattc atagaaagcc ttcttttttc
    23821 ttcttccact tttttaggat ggattttaac tgctagcgca gggtttcttt gaatgtattt
    23881 tctatcgatt gcatagttta gcataacaga tagagttgtt tttgtttgtt ttgtgtagtt
    23941 cagtgagagg tcaccaaacg tatacatatc ttcaattatc ttattaatga gtgtctcatc
    24001 aatgtttcta acgatcgtat catcgcttat gtgcttagaa acatgtttca tcatcattgg
    24061 aaccttcaaa tagctagtac gtttaacatg ctgcttataa tattcatacc attctttata
    24121 cagctcacca aaagtgatat ctgatttatt gtaatcttcg agtgcttctt taattttttt
    24181 atctagaatt ttctgagctt ttttccacgc ttgtggtgaa ttacttgtaa gtgttgtaga
    24241 tttttttcgt gttttttctg tataaggatc tacatatctt tcaataaact tgaattggcc
    24301 atctttggtc tgttcaaccc acacttttaa catctcctat catttgctat aataggcata
    24361 acaaatagac ctatataggt ttgttttcta aaagcacgct cttactctgg acggtggggc
    24421 gtgtttttta tttattatac agttgcttta aaaatagctg ctttttcaaa tgatactaaa
    24481 ggagagaagt ggatttctat ttctcccttt tggtttaacg tgaaatgagc agttacatcc
    24541 atttgttttc ctggagcaac agatcccatt gtattttcgt tggcatatgt ctctgatttt
    24601 ttgtcatctg gtccatatac ttccacatct gtacctacag ggatatctga atcgccatca
    24661 ttttttacgg tataagtaat ttttactact tgtgcaggtt ggttttcttc aaattgattt
    24721 ctttcatcag ttagttctac actatttagt gtatattcag catcaccaac ttttacagtg
    24781 tcaccaatct tatagaaggt atcgcttttt tcttctttag aagatgaagc agaggttgaa
    24841 gatttagtta ctttttcgcc accattatca cttgctttat tactatctga tccaccattt
    24901 aaagcagaac caataattat aattaaaatt actgctaata cccaaaacca tacttttttg
    24961 taaaaaggtt tactaacttt atacattttt ccgtcttgac ccataacttt ttttgccatt
    25021 taaatattcc tcacttcttg ttataatata tttgcgatct cagaaatgag gtatgagtcc
    25081 gtgtgccagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa taattcttga
    25141 tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca ttggcagaca
    25201 gcataacaag tgtatctgag cttattctga atagaataat actccataaa tttttcgaga
    25261 ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa aattagaata
    25321 gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa
    25381 actgaatatt caaaatgaga agcggtgaaa tgcgcaagtt catgagataa atgaaaggct
    25441 tctgcagttt cgccatatag attttcattc aaaaaaatga ttctaggctt cggataatag
    25501 aagccaggct ctttcatttc catgtaaact actttcaaat tgtattcgct caacatttct
    25561 ttcaatttca aatacataca acccatcact ccaactattc attttcctct aaagctttag
    25621 caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca aaagaaaaaa
    25681 cagtatcgtt ttctgataaa tccacatgtt taggggtttc tctttcttct ctacctagaa
    25741 ggtagtctac agagactcca aagtaatcag ctaaccgagc taactcaata gaattaggag
    25801 tttgtctttt ccaacttcct aaatatccat tcgaataccc gaaattcatt tcgagctgtc
    25861 taatggataa tcctttttct tttgctaact cctttattat ttcgtaagta ttcattgata
    25921 aatcaacctt tctgaatgct tacaaaaaaa gtttagataa ataatgtaaa aatagttgac
    25981 ttatatacat tattaatcta tactatgttt tgtaaacaag tttaacaact aaaaagacaa
    26041 caaaaaatac tattgattaa taaatgccaa ccgccaagaa agctttttaa atcaaagttt
    26101 atatgtctta tttaactatg catttagtat agattattaa tctaataaag tcaacgcagt
    26161 tttttaaaaa gttgttaaat ttgtttacga atataaaaga aaggagagaa gaatatggaa
    26221 aaaacagtct cggcaaaaat cgaagatttg aaattagaca tactgaaaca agcaaaagtg
    26281 gcgatggaac acgcggtaga taaagaagac tctgccatgg ttgcagccat agcagagatt
    26341 ttagctcacg tttagtcatc gtttttacgg tcttctggaa gctgaccata gataacagca
    26401 taatgaatat ctaaataagc ttgaacaatt tcttttggcg aaattgattc gcctttagta
    26461 gatacttgtg attcgtgatt tagccatgca acaactaaat ccgcagcgat ttcaaacggc
    26521 gttacacttt gtgtcagttg ccataaagaa tttcacaata tgtatggtcg tggtaataat
    26581 accaaagagc aatttgagga atttatggaa attaaaaaat acaaaaaatc atttattaaa
    26641 ggaggctaaa caatgaacaa ggaaatgagc caaacaatca aggtacaaaa aatgatagat
    26701 gatctaacac atggaattga tagccaagcc gataaaatca tcaaggaatt gcaaggacaa
    26761 aaagttaagg atgcaaaaat gcttctcaag actatcaact ttgaaatgaa tccaacaaaa
    26821 agaaagctcg ccgatgtgtt ggaagaaaag ttggcttccg ctatcaacga gcaagaatta
    26881 ctatttgaaa ccgatacttt taacagctga tgttttatag atgacagttt catccgaatc
    26941 tatatcatcg ccgagagaaa agaatgttgc ttttgacaaa aagccgataa gacctacaag
    27001 cggtcttgat gttgcaagag tagatacccc aatatcacct tgcaaggtat tcacgtaaat
    27061 ttgagaagaa tagaacattc catctttttc ttcgatattt gacgcatcat cgtatccaac
    27121 gaccaaagta tcggcaaata ttttgatttt ttctccatca agcatagtaa ttacaaattc
    27181 tggtttcata aattttcacc tccttaacaa ttatttcagc ctgtcacact gataaggaaa
    27241 ttataccaaa gaaaggaatg agaaatatga atacaccaca aatttttaat ttcgagcaaa
    27301 acgaagttcg aactatttta gtaaatgatg aaccatattt tgtaggaaaa gatgtagcag
    27361 acgttctagg atattcgaat ccgcaaaaag ctattcgaga tcatgtggac ttggaggata
    27421 agacgcagaa cgattcgttc accgtcaatg gaacagcagt tgttttgatt aacgagtcag
    27481 gcctttacag tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg
    27541 taacgagtga agtccttcca acaattagaa aaacaggtag ctattcaaac gtacctcaaa
    27601 gttttgcaca agcattgcgt ttagcagcgg atttagaaga aaagaaccaa ttactcgaac
    27661 aacaaattgc cgagtacgaa ccaaagatta gctacttaga tacgattctt tcatcgacag
    27721 atacggtagc tacttctcaa attgcagctg attacggaat gtcggcaatt gctctaaaca
    27781 aattgctaaa cgagttaggt gttcagcata aagttagcgg acaatggata ctttaccgaa
    27841 aacatatgaa ccaaggatac acaaaatcgc acacaagtga gataccgaaa gccgatggcg
    27901 gcactaaagt tgtaatgaat accaaatgga cacagaaagg gcgagtgttt atttacaact
    27961 tattaatcgc agagggctat taccctcaaa tggatttaga ggaaattggt tagaaaggag
    28021 ttttagtatg actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata
    28081 tgtcgaaagt tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa
    28141 aagctactta tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat
    28201 agattcgatt atcactatgt acgaattgta gagggaggta aagtcgttgg aagataaaat
    28261 cattcttaca aaatcagagc ttcaagaaat gttaaacatc gagtatggaa cgataatttt
    28321 taggagggat aataagtgaa aaaaccaaca ctttcagagc tgatagaagc tgctgagaag
    28381 gcagtaaaac cagacgactg gtaccgacaa agtttaatct tggagaagtt ccacggcatg
    28441 tcaaaaacta ctttagttga atactgcaag gaaatggaaa caattcctga atttgcagaa
    28501 ggaatacttc gtccaggtca ctcaaccaca tttattcatt accatacttt tatttggttt
    28561 ttaaagtgga aagacgcaaa caaatatcgt gtaaaaagtt tatcgccttc agatgttttg
    28621 aaggaggcaa gttaatgggc aaattcaaca gagcactagt attcagcgca ccgctaatca
    28681 tctacgcttt aggcctctgg ggaagcagac aagcgttgat agggacaatc gtttacatgg
    28741 tttggatttt tatggggctt gatgaagctg agcacagagc gaaaaaagcc agtcgggagg
    28801 gactgactaa aatatgattt ttttattttt gttcctttgg gctgctattg cgtacttcat
    28861 ccagctcctt gttgtcaaag ttgctcttat ttcttttagg aagaacaaaa gaaaatagcg
    28921 ctataaatga aagataagta gccgctacat taaactgttc ttttgaaaaa gttacagtca
    28981 tcggaatgat aattgatatt gaggaagtaa tcttaattaa aaatatagca atattttcaa
    29041 catcgcgttg gtttttattt aacattgtcc aacatgtttt agcaacaata aaaataatta
    29101 cacatgatat tagcacacag actgaaaaaa gttttagaaa tgcgatgtag tcagtgttat
    29161 tgaactcctt caaaatacta tttttattca atgtaataaa aggaattttt ttaattgaag
    29221 acaggaaaga taaggtaata aacagaatta tatatatggt taaagtcgca gtaagaatgt
    29281 aattgagctt tttcctcata gaatcacctc actttctaac gaaaattata ccaaaaagga
    29341 gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgta actacgagct
    29401 tacaagtggc agaaagtttc aaaaagaaac ataagcatgt actagaagca attgaatcaa
    29461 taaaaagatc ggtcgaaaat tcggccaatg ttgaagatgg gtccaatttt ggacagatgt
    29521 ttgtggaagg gaacgagcca gactcatacg gaagaagtcg gagagtttat ttcatgaata
    29581 gagacggatt ttccttgcta gctatgggat tcactggaag taaagcaata aatttcaaac
    29641 taaaatttat tgaagctttc aacgaaatgg aagatgttat tcggaagaat actgttcctc
    29701 aaacaattga aga
//
LOCUS       DARDHU010000023        28640 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000023 DARDHU010000000
VERSION     DARDHU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.023"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            7..624
                     /locus_tag="SDD03_001095"
     CDS             7..624
                     /locus_tag="SDD03_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303559.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="SDD03_001096"
     CDS             1644..2813
                     /locus_tag="SDD03_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4303560.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="SDD03_001097"
     CDS             3286..3840
                     /locus_tag="SDD03_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4303561.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="SDD03_001098"
     CDS             complement(4013..4486)
                     /locus_tag="SDD03_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4303562.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="SDD03_001099"
     CDS             4498..5034
                     /locus_tag="SDD03_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4303563.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="SDD03_001100"
     CDS             5074..5445
                     /locus_tag="SDD03_001100"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4303564.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="SDD03_001101"
     CDS             5729..7000
                     /locus_tag="SDD03_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4303565.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="SDD03_001102"
     CDS             7245..8270
                     /locus_tag="SDD03_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4303566.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="SDD03_001103"
     CDS             8564..9673
                     /locus_tag="SDD03_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG4303567.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="SDD03_001104"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="SDD03_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="SDD03_001105"
     CDS             10668..11492
                     /locus_tag="SDD03_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4303568.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="SDD03_001106"
     CDS             11524..12834
                     /locus_tag="SDD03_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG4303569.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="SDD03_001107"
     CDS             12982..14241
                     /locus_tag="SDD03_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4303570.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="SDD03_001108"
     CDS             14251..15123
                     /locus_tag="SDD03_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4303571.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="SDD03_001109"
     CDS             15135..15965
                     /locus_tag="SDD03_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4303572.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="SDD03_001110"
     CDS             16005..18188
                     /locus_tag="SDD03_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG4303573.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="SDD03_001111"
     CDS             18249..19469
                     /locus_tag="SDD03_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG4303574.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="SDD03_001112"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="SDD03_001112"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG4303575.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="SDD03_001113"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="SDD03_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="SDD03_001114"
     CDS             22431..22640
                     /locus_tag="SDD03_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG4303576.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="SDD03_001115"
     CDS             22633..22782
                     /locus_tag="SDD03_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG4303577.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="SDD03_001116"
     CDS             22936..23601
                     /locus_tag="SDD03_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4303578.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="SDD03_001117"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="SDD03_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG4303579.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="SDD03_001118"
     CDS             25304..25537
                     /locus_tag="SDD03_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG4303580.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="SDD03_001119"
     CDS             25534..25941
                     /locus_tag="SDD03_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG4303581.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="SDD03_001120"
     CDS             complement(27078..27596)
                     /locus_tag="SDD03_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG4303582.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27963..28211
                     /locus_tag="SDD03_001121"
     CDS             27963..28211
                     /locus_tag="SDD03_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303583.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9843 a         4314 c         5247 g         9236 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcggtact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagcttg
//
LOCUS       DARDHU010000024        27574 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000024 DARDHU010000000
VERSION     DARDHU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.024"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(192..662)
                     /locus_tag="SDD03_001122"
     CDS             complement(192..662)
                     /locus_tag="SDD03_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG4303584.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="SDD03_001123"
     CDS             992..2632
                     /locus_tag="SDD03_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG4303585.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="SDD03_001124"
     CDS             2786..4108
                     /locus_tag="SDD03_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG4303586.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="SDD03_001125"
     CDS             complement(4176..4610)
                     /locus_tag="SDD03_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4303587.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5512)
                     /locus_tag="SDD03_001126"
                     /pseudo
     CDS             complement(4675..5512)
                     /locus_tag="SDD03_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(6254..6880)
                     /locus_tag="SDD03_001127"
     CDS             complement(6254..6880)
                     /locus_tag="SDD03_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303588.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7175..7510)
                     /locus_tag="SDD03_001128"
     CDS             complement(7175..7510)
                     /locus_tag="SDD03_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG4303589.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7497..7781)
                     /locus_tag="SDD03_001129"
     CDS             complement(7497..7781)
                     /locus_tag="SDD03_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG4303590.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7837..9360)
                     /locus_tag="SDD03_001130"
     CDS             complement(7837..9360)
                     /locus_tag="SDD03_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4303591.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9353..10528)
                     /locus_tag="SDD03_001131"
     CDS             complement(9353..10528)
                     /locus_tag="SDD03_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4303592.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10532..10717)
                     /locus_tag="SDD03_001132"
     CDS             complement(10532..10717)
                     /locus_tag="SDD03_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303593.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10683..12377)
                     /locus_tag="SDD03_001133"
     CDS             complement(10683..12377)
                     /locus_tag="SDD03_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4303594.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12374..12847)
                     /locus_tag="SDD03_001134"
     CDS             complement(12374..12847)
                     /locus_tag="SDD03_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG4303595.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12916..13071)
                     /locus_tag="SDD03_001135"
     CDS             complement(12916..13071)
                     /locus_tag="SDD03_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG4303596.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13205..13585)
                     /locus_tag="SDD03_001136"
     CDS             complement(13205..13585)
                     /locus_tag="SDD03_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4303597.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13589..13804)
                     /locus_tag="SDD03_001137"
     CDS             complement(13589..13804)
                     /locus_tag="SDD03_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303598.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13807..14214)
                     /locus_tag="SDD03_001138"
     CDS             complement(13807..14214)
                     /locus_tag="SDD03_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG4303599.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14480..15955)
                     /locus_tag="SDD03_001139"
     CDS             complement(14480..15955)
                     /locus_tag="SDD03_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG4303600.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15945..16793)
                     /locus_tag="SDD03_001140"
     CDS             complement(15945..16793)
                     /locus_tag="SDD03_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG4303601.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16830..17192)
                     /locus_tag="SDD03_001141"
     CDS             complement(16830..17192)
                     /locus_tag="SDD03_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303602.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17193..17351)
                     /locus_tag="SDD03_001142"
     CDS             complement(17193..17351)
                     /locus_tag="SDD03_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303603.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(17492..18116)
                     /locus_tag="SDD03_001143"
                     /pseudo
     CDS             complement(17492..18116)
                     /locus_tag="SDD03_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18113..18424)
                     /locus_tag="SDD03_001144"
     CDS             complement(18113..18424)
                     /locus_tag="SDD03_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303604.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18468..18761)
                     /locus_tag="SDD03_001145"
     CDS             complement(18468..18761)
                     /locus_tag="SDD03_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303605.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18955..19602
                     /locus_tag="SDD03_001146"
     CDS             18955..19602
                     /locus_tag="SDD03_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4303606.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19663..20808
                     /locus_tag="SDD03_001147"
     CDS             19663..20808
                     /locus_tag="SDD03_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4303607.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20889..21194)
                     /locus_tag="SDD03_001148"
     CDS             complement(20889..21194)
                     /locus_tag="SDD03_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG4303608.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="SDD03_001149"
     CDS             complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="SDD03_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG4303609.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21647..21913)
                     /locus_tag="SDD03_001150"
     CDS             complement(21647..21913)
                     /locus_tag="SDD03_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG4303610.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(22168..22893)
                     /locus_tag="SDD03_001151"
     CDS             complement(22168..22893)
                     /locus_tag="SDD03_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG4303611.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(23254..23324)
                     /locus_tag="SDD03_001152"
     tRNA            complement(23254..23324)
                     /locus_tag="SDD03_001152"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23290..23292),aa:Gly,seq:ccc)
     gene            23441..24193
                     /locus_tag="SDD03_001153"
     CDS             23441..24193
                     /locus_tag="SDD03_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG4303612.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            24209..24688
                     /locus_tag="SDD03_001154"
     CDS             24209..24688
                     /locus_tag="SDD03_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG4303613.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24939..26519)
                     /locus_tag="SDD03_001155"
     CDS             complement(24939..26519)
                     /locus_tag="SDD03_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG4303614.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(26512..27255)
                     /locus_tag="SDD03_001156"
     CDS             complement(26512..27255)
                     /locus_tag="SDD03_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303615.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(27431..>27574)
                     /locus_tag="SDD03_001157"
     CDS             complement(27431..>27574)
                     /locus_tag="SDD03_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG4303616.1"
                     /translation="INTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIEN
                     ESF"
BASE COUNT         8102 a         5577 c         4624 g         9271 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     5101 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5161 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5221 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5281 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5341 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5401 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5461 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5521 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5581 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5641 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5701 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5761 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
     5821 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
     5881 tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa caaatggcaa
     5941 aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct gattgaacat
     6001 cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt ttttatatct
     6061 tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgagaa acccatatca
     6121 aagaactata aaggttatca aaacagcgtt tatgactaaa tgaattagag ttatctatca
     6181 ctatttttca agcgatttaa accaaatggc atacaatatg gcatacaaaa acttcaataa
     6241 gccgtggggg tttttaaatt tcttcttgtt ctttaaaatc aatgagtgga gctacataat
     6301 ttgaatccac tttttcaaac gtcacttttt gcatcgacga atgttttctt tcccaagata
     6361 acgtaaatag atttgactct ggacttataa agtctacttc accttctagt attactttct
     6421 tatgcaaata ttcctctaat aaatatagac ttgctaaagc atacaacaca ttttccaaat
     6481 ttgcttcctt ataatttgaa gaacgaccat gttttactct gttatatatt ttccaccaat
     6541 ctggtgtatc ttcaggccac ccatctccaa aaggttttag tgaagtcaat gttaaagaat
     6601 tcataaccct aacaccatta tccaaacaca ttaattcgct agatttaata acagctttaa
     6661 aatcagttat gcttttattg ctactgctat caaatccgca aagttctttg aaaataatct
     6721 cacactccga accaatcgct agtagtagtt tttgatattc aatagagaat gttttgaaat
     6781 tattctcatc gagcatcaca aaatctgtgg tattaataaa ctcattttct aaatgtagat
     6841 aatacttcca ataaaaattt gtaaactctg ctttgtccat taaatcacct cttttgaatt
     6901 ataacaaaaa aaacacgtcc acaacagacg tgttctttga tttctacaca atcagcttgc
     6961 caaaaccatc caatcagtcc tagcatgcct gtattaaaat tgaacgttca tcatctatca
     7021 tctttcttag tccagcatgt ttgcaaccac acctacaatt ttcagtccaa caatagattt
     7081 cgactagcta agtcttgcaa aattcaggca gctaaccctt catttactac cttaattata
     7141 ctaaataccc acaacgttta caagtgtaat cattctaaga aatctgttta gctatgatcg
     7201 ttgtataatc tttaaacgcc gttccttcat tgattgaaat aattttataa ctcttgtttt
     7261 tccaagctac tttcatatcg tttgttagtt cataatcttg atcgtatcga ataataaacg
     7321 tcaacgttcc ttcaagaatg gtacctacac tagcaagcac gtcttttagc atttgtgaac
     7381 gcacattagc ccaacattca aatacagttt cttcactatc caccatctgc ccatcttcat
     7441 cttttacccg ttttgtcttt ttaaacttga ctctctcagt caattcattt acattcttaa
     7501 tgattggcat aataagcacc tctcatttgt tgtactaatg cttgtaccgt tactggtatc
     7561 cgttcgctgc tggcttcttg acggttcaaa taccaatgtt gagtaagtaa tgacaccgcc
     7621 aaatcaaatt gctgataatt atccatatct ttatccgttg cgctactatc aatagcactt
     7681 tttatatatt cgcctgctgt atcaatcaat tgttgaataa agtcatcatc aatagtgtga
     7741 tcgatccgca tacttttttt tatgctatcc aaaattacca ttttatctct cctaatgaaa
     7801 aaaaggcagt gggataaacc gccgcccaat atttttctat acttttggtg ttacaactgg
     7861 cgtaaactca atatacactg aagcattttg atcaattttc ttataatcgt tgcgaacgat
     7921 cactgcaagt ccttgtgagt agtaatcgaa tttttcccat tgagtcgtta cttgatttct
     7981 acgagcaaca aagatagatt gtgcaatatc ccccataatc atagggaatg ttccagcttt
     8041 aggattggca aacaatgtat ctgcaatcaa tactactggc ataccgaata atgatttacc
     8101 actaggtgct gtcacatcgg gttgtaaaat gtaacgtcca tctgaatctt tcaatgtatc
     8161 taggtggtaa tatccgcttt ggtttagaat taccatttta tttaatgctg ggtctaatgc
     8221 cacattgtat agttgtttca aatcgtccaa cgtagcagcc gttttcttag tgaatgtttt
     8281 taataaatcc atgatatgct tattgtcagt gttttctacc aatttcgcta actgatcttt
     8341 gacctcttgt acaatattca ctgctgaatc ttccacaacc tcatttgata aggcaatctt
     8401 accagcacga gtttctactt tataatcaac tgaagtaaac atttcagcgt caatatcacc
     8461 aatttcagca agttcagctt ttgttgctaa cactgcttgt tggttagttg ctactggata
     8521 tttaccttgt ccatttgata ctgtttttac tgttgcatat tgagctaagt tataatttga
     8581 acgtttcaaa tcaaagactt caccgattac ttcttcggga acaactgccg ctgcatttac
     8641 agtagtgact ccatcacgca cttcgccttg tgaacggata tattcttcat acccacgcaa
     8701 ttctgttttt tcgttatcga ttaaagtttt ttccatgtta ttactctcct tttgtgcttt
     8761 agaagtggat tctaagctat tgtttgtttg ttttgcttgg ttgttactca taaacgattc
     8821 gtaagaacgc ttatttactt ggacatttga tgaatcataa gcgggaacgg tcacgacact
     8881 aatttcatta agtgctttga ttttatctat tgatcgttca attgtgccat cttctttttt
     8941 gtcgaatgaa tctaatccaa gcacgaaccc aaaggacata gaatcaacaa ccccttttga
     9001 gatattctca tacacatcat ttgaataggt cgtgtcggtt agctctgctt caaaatgtaa
     9061 tcctgtatca tcgacattca atttcaacgt acctgctttg acactagcta gtggctttga
     9121 ataatcgtgt ccatataata agaacacatt tgataaatcc acctctttta atgcttcggg
     9181 tgtaatcact tctacaaagc cgcctaaatc ttttgacggt tgcccgaatt tcaacgcata
     9241 gcctgaaata gttttgccgt ctgttggttg tttttcattt tctttctcta ctttgggaga
     9301 atcggctgtt agctcagctt cttctgtcag ccgtttttcc ttttcttctt cattattcaa
     9361 ctggtgtaac tccttcctgt tcttttaatt gatatttctc caacgtatct aaatacgtaa
     9421 agttcaaact tgctagtaaa cgatcgccac catccatggg gggtagcccc attttcgacc
     9481 gcccctcatt aatcgtgaga agtgaccctt tgacctgatc caatacattt tttacttttg
     9541 tttcaggatc agtctctagt aactgatcgg aattaaagcg gatattggtt tcaagtttcg
     9601 tatctaattc actcacaaac acattaaagt aatgaatcag cgtactttgg atatactgga
     9661 gattactttg aacggtgcta gaatgttcat tttcaacgcc taaacgctca atcggtacac
     9721 caaacgcctt ggcaatttgt tttgtgttcc aatcattgga attgataagc ttcaaaacat
     9781 cggtgtttat ttctaatgtt ttgtagtcca ttgtttcatc aagaatgatc gttctaagag
     9841 cattatctcc actacttgaa ccgttggctt cttcaaactt ttcacgaatt gcgttctttg
     9901 ctgatccgtc taaatctgac ttatgaacct tcaaaatacc tgacccactg acaccacgag
     9961 taaagaaatt gtgcaatgtg cggttgccag cttgttgaat tttcagttca tcacgtaacg
    10021 cataaagtgg tggtaatcct gttaaaccat cttgggtgaa atatttgaag tgcaaaatat
    10081 cgctagactt cacacgtctt tttttatcac caatctgata agacaacgta ccattatcta
    10141 attgagtaac cgttacactt gagttaggta ataagtgaat ttcttctacc ttttcaccgt
    10201 tgcgtttaat ctctgcaaag ctattaccgt ttagcaacat attgaccgcc aaagcaaact
    10261 ttaaatgcca accatccatt tctgaattag gtttctcgtt tagtaacttc accagttcat
    10321 tatcagcttg tggcatattg tttttgacca attgaatcgg acttgaagca atatcactag
    10381 caataattcg aacggctgca aacacatcac tatttcttaa tgcacgaacg cttgtaaagc
    10441 tggtggtgta atcatccgat tggattgata ccacatgttg taggaaagga tctcccgttg
    10501 tattttgtct cgctttaaaa aatgccatct tttaacctcc cttctgttct gtatttagca
    10561 tgaccgctac cccaataagc actatgccca gcgctaacaa gccatagtag acatttgtta
    10621 gtaaggtgat agcaaccaac acacacacca atccaagaag taaaagaacg gtatgaatgt
    10681 aattagaaag aaaattcttc acttgtataa aattcattgt ctgctttcgc tccttccgtt
    10741 gtggtgaagt aatccattgc aaacacatag gcattgatta atgcagctat tggatcaatt
    10801 ttgttactgt tctttgattt attaatctga atgccgttgt tatccgtttt taaaatagcg
    10861 ttgttcactg catgggtgag aatcgtattt ttattgtgaa taacgttgcc ttcataaact
    10921 tgttctctaa aagttcgggt cggaacgttt agagtaatcg ttccttgtct tacttctaac
    10981 attgggtagt tggctttttc agctttactg ataagcgaat tagcgttata cgggtcataa
    11041 caaatcccca tacattccaa atcattttct tgaatcaaat cttgaataaa ctgaaatact
    11101 tggtcatagt ccacgattcc actttctaat tgagtaatgg aacactcacc agctttttct
    11161 agttctcgat aaggcaaacc gtcgcgcttt tctttgtctt gaagcccata tttggtggct
    11221 acaaagctat gtgaatcaca ataaagttcg ccgttatcta gcggtacgat ccatgaaacg
    11281 ctagttaaat catctgtttt agataaatcc actccgatat aaacgggctt gcctgtaata
    11341 tcttgtggtt ctacttcggt tgcctgccag tcttcagcaa tcatatagct gtcctcactc
    11401 gcttgtctcc ataaattgaa gtttttcacc aatacagaat taaggttatt ttgtttcaaa
    11461 gcaaggttca catcatcttg aatggttgga atcattactt ttttgatttc ttcactttca
    11521 aaaatcggat tggctttgat ccatttctct tgatcgtgaa tttcttcggg atcgtccaat
    11581 tcccatatcg ctataaagta cctgtcggct tgttcttcgc cttttagcac acgatctaac
    11641 aataagtatt cctcgtacat aggaacgttt aaatcaagcc cagcggtact aataacacac
    11701 aataagccgt tcttttgttg ggtcattcct gacttaatga cgttgtacgt tttacgagtt
    11761 tttgcttcgt gccactcgtc aagaattgcg gttgttggtg caaacccatc tagcgtgctg
    11821 gtttcactag ccagtgccat agcgaacgaa ttagagggca agtgctgaat cttagagttc
    11881 ataattttca gttgtggtct taaaaactta ctagacttga ccacgtttct aagtgaatta
    11941 gacagcatat catagcccaa tttagcttgt ttcaaagcgt tacttacaaa caatacttgc
    12001 cttgcttctg ctggttcttg ttccataatc aacgcattgg cagccatgcc acttgcgata
    12061 tacgttttcc cattcttacg gctcatactg gtaaacgctc gattgaatcg cctgtaattc
    12121 ccttctttag tacgccagcc ataaagactg cctagaatga atttttgaaa tcctaacatc
    12181 ttgattgctt taccgtccgt agatggtagc aactcaatga atttaatcgc tttggtggct
    12241 tgttcttcat cgaacacata agggaagcta tctgacttag aacgctccaa atcgcttata
    12301 tgacgctcac acgcttggat aattttcttg ggtgcaatca gcttacctga taggactttc
    12361 tcaatgtaat cattcatgaa ttcatcaact ctttgaacgg gtcttcgggt tcatcatttt
    12421 taaccttgtt caaagctagt tttgatcgtg attctaaggt catacctagt gaggtggctt
    12481 gttttttcaa atcactcata gcttgtgatt gaacccgaac cgctggattg gctttcttta
    12541 ctccgttttc gcatgtgata aacgtacctg attttcttat ctcggcttcg gctgttttca
    12601 ttcgagcaaa ggctaaacaa taggctacca atgaaccata atctgcttca ctcaatggat
    12661 aatctttttt taatgttggt acgatacgat tccattcact tctagcgctc gctgccatcc
    12721 agtcaggggg cgtgatattt attaatggtt gctgcttgaa caattcttct ctagcgtcca
    12781 cacggattgc tttttcttca cttgatatat tgccttttgt atcgtgtaat attttagggt
    12841 gtcccatgtt gtcctccttt cagacaaaag aaaaaggaac gtaagcaatt aagctcacat
    12901 tccttcgttt ttcgatcaga atttttcttt atctaattta tctatataaa tgatttttcc
    12961 gttctgcatt tgaatggtca cttgaccaaa atgaggtaac tcaatggtgc gctgtttccc
    13021 tgttttatca gtaataacaa ttgtattttt caataaatct cctactttca ttatatactt
    13081 tttcacttgt gattacaagt gtaatcgtta tttgtaatta cccttatgcc ttttcacaac
    13141 ggttatttgt accaaaatga gtaggcatcg attgtcagta agtttcacac tatgccccct
    13201 taactcaaag ggtactcttt tctcgtcttt gtctttcatt ggctgtcttg gaattatggt
    13261 gtgcatggca taaagaacga aaattagttt catctaaccg tctattccaa tcatctttta
    13321 gttcaatttt gtgatctaca acatcagctt tccttatcaa gccttcttct aggcaatcct
    13381 cacatactgg atgatttaac cgatacaagt atgaagcctt tcgccatgtt ctactttgat
    13441 agaattgaaa atatttacct tcgctttgct ttctttcttc acgcttaatt gccgtttgct
    13501 ttgctttatg cttcccacaa tacttctgcc tataatcaat caatgtttta catccagcat
    13561 gattacattg ttttttaact gccatagatt accaaccaaa gcgatctcgt tcgtcagaat
    13621 catcttgttc ccattttgat ttgtattcaa tataaggcga aactatcttc aagcacgttc
    13681 cgtctttaaa attaacttta gtgattttat cttcctcaaa actaataaag tctacatctt
    13741 cttgagtgtt tgaatactca atgtgtttct caccatcgaa gtatctaata ctttccatat
    13801 gcataattat tctacctcca ataaattagt tatcaattca tcatctgtta gtagcaaagc
    13861 caagaactta tcatggatgt ttaaaaacaa attatcctca ttcgatgtaa gctctcgttg
    13921 tctcgctcga tcatataggt taagcagaca attaaattca tccattagct cacctgtatc
    13981 atcatagttg ccactatcta ccaatgatcc cactactagc gattgacacc acgctactga
    14041 ctcctgcagc caatcaggtt cttcttccat tatctttaat acttctggat caaatgtgga
    14101 ataatcttct gttaccaatc ccacactaaa tgccagcgtg ttcattagaa accgatccat
    14161 ttcatctgta tattgaaagt tcggtgcttt gtttgttgaa ataatattga gcatttactt
    14221 tcctccttga ttcgtaacac gtggtaacac ctacgtaaca ggtacccgtt accactcaat
    14281 cccaacgggg acaagggttc tagcgttttg gtaacaggta acgggtaaaa tccagtaaca
    14341 ttattcccca gcatattagc taactatttc aaaagtttta aggaaataag gtgttacccc
    14401 gttaccaaat tgctgtatcc cttggggcag taagggtaaa ctagtaacgg gctaggtgtt
    14461 actgacccgt tactacccgt tactttttgt actttccagc tcgattttta ttttcatagg
    14521 catttgactt attgtaaaat cctcgtcgtt tatttctttt tggcagttgc ttccgttccc
    14581 aaccaggaat actgctaata atcaaggcaa tctttttagt ttctgcatct gatcgaccat
    14641 tcaataaatc ctttgcttgt ttatcaaaaa cagcttccaa aatatcagcg gtgagtacgc
    14701 tatcaactaa ataaaactct tgttgcatag tagctccatt atagaaatgc tgtaaatttt
    14761 tctcattaca tttactgtca ggatagttct gataataaga acgtctcgcc cacgctggag
    14821 cttcttccca tttatacggg atttccattt ccaaatattt agtgattgct tctttaattg
    14881 gattctccat cttggcatcc tcttgatagt cttttgcaat ttctaagact tctttatctt
    14941 catcgggact aaaatagatt cgttgtcctt tatcaaaaag cacctttgct tcagctaaca
    15001 cttgaagaaa atattcatcc tttgttttaa acggattcat ctttggctta ttcttacaag
    15061 gcaaaggata aaaacggcgg tttcctgtat catctttcaa atactgccca tcattcgacg
    15121 ttccaataaa tacacattgc cttgcccaag ctatgacatt ccgaccataa ggaggtctaa
    15181 ttttatcgat cttggcactg atgaagtttt ttatctgttc aattttagtt ttgttcattg
    15241 aagataattc tccaagttca ataattacat tgccttgaag ctgcatataa tcatctttct
    15301 tttcacctag cgaatctaat gaatcggtga aaaaatcatc cgtgtaaagt aatgctggaa
    15361 cggtactctt accaatccct tgcggtccat ccaaaactgg aacgatctca aactttattc
    15421 ctggtttata gatacgtgcg actgatccga ctatccaacg ttttgttact tctcgtgtgt
    15481 aaaggttgtc gtctacccct aaaaggtcaa taaacagtgt ttccagtcta ggctgttgat
    15541 cccatttaac tttttcgata cgttctttta ctgggttgaa aacattcttt ctagccacat
    15601 ttactactgc tttgtgtatt ttgttgtcat caaaaactac attgtagttt tcttcaatat
    15661 atgccgttaa ggcatcctca aaactgtctt cccattctcc agcggtacga ttgatccaag
    15721 gagaatcttt taataggtga atgtatccgc taaattcgtt ataagcaatt gttccagcta
    15781 aattgctatc attggttaaa atcaattctg cattcacaac actgttactt ttaatctgat
    15841 cgtctttatt gcatcttaat cgtgacatcc aggttttcaa ttcaatgata ttgtcttcac
    15901 gctcgctggc ttcgggaaag tctaaaatac tatctaattt atgcttatga gccatgagct
    15961 ttccccctca aatctttttt aatcatcgat cgaataattt gattcacttc tttatctttt
    16021 agcggtggtt cattgtacgc atttgctgta taaatcaatt ctgctaaagt ttgattatct
    16081 acaccgtgcc atagtaacca accgcataac ttagttaaat gatcgtttct actaccttcg
    16141 ccagcgcctt gaacaatcat atctaatttt tttgctgtcc ctgtttttgc tacgggtcgg
    16201 tattgagtct ttatttcttg cggttttttt agcagtaggt tcactagcca atcgggagct
    16261 ggtgcaatat tatttagaga tacgttttca ataggttcat atgctctccc aacacttctt
    16321 gaaataatca tagttggcgc tattggaact cctttagtga taatatcaat cccgcttaaa
    16381 cttttacctt tatcatcacg cccgaagata tcagcaaatg cacctgtttc ttgttggata
    16441 attacaccat caggaatttt aaagaagaaa tggattcctt tacctggtgt gatttccgta
    16501 taagtagaag gaaatggatc atacattgaa gcaagttttt tataattctc aacgccgttt
    16561 cgtgtttcag aatgttgatc taggtcaact accaccattt gatgatcttc aagcattaac
    16621 ccaatatttc tagttgcatc ttccgtccac cattcattaa tttgattaga atcgttagtt
    16681 gcatcattcc aaccactaga gccttttatt ggaattttag ttccaggaga taagggataa
    16741 attgaccatc ccgacttttg taattccgtt gctgcttgat aattatttgc caataaacct
    16801 ctcctccttt ttcaaaatac tggtataaat cattcagaca cctccatgag cttcaactgc
    16861 tcaagttcgt atttcaaccc atcccgaaca aattcagcac cttttaattg attctgatac
    16921 tttacccatg tattaaatgt tttcaccctt gcttcttgca tatcagtaat gtttaaccat
    16981 gcggttaaaa tctgattttt tagctcgttg atttctcgac aaattatttt gtgttctgca
    17041 tgttttttga gtacatacaa aactgttagt ttttcattga atgaatttcc aaagtaggtt
    17101 ttcccgatat tattttctag ttcagcgatc ccttgattaa tttcctctaa ttcaaggcta
    17161 tcgagttggt ttaatagttt tgttttgtac atctacttat gccctccagt tcttaattga
    17221 tagtcgtact cgtcataagc agtgaagaga tataacgcta atcctgcaaa gatagctatt
    17281 tttgcaatta atggtaataa gccgaaaatt ccgccgatta aaatgcttcc tataattaag
    17341 tttcgtttca taatggttcc ctccgatttt ttaatatggt ataatcggaa caaagaatat
    17401 cgggttttat cccaattaag ttgcctttat cctgtccgcc aagactgtta tggataaggc
    17461 tttttctttg ttctcgatca tgttaatgct cctaagttgt ctgcaattca gcagaacgat
    17521 tataaatttt ctcactcact gaaaactgca acccatattt ttctttaaca gtcattaatt
    17581 ctactgtctc atctaaaata ggttcacgat caaccaacat ttcaggagtc atatctttct
    17641 tttttaccat tttggaatga ccataaatag ttgatacagc tttattggct atcacattcg
    17701 ctttaatgta gtctactggt tttggttcag taatagcgtg gcttaattta gtcattgctt
    17761 ctttttggtg ttctttgtct agcatgcgga atacttgaaa gccttcaagt cctgtggact
    17821 ggcgtagttc tttgatgatg tcgaaaatcc agtcttgaaa tgcctcggct tcttttttac
    17881 ggcttttgaa tgccatgcgg taaatatttt ttttcgttga tgatgtttac atcttgtgtt
    17941 ctgcccaatg aatcgatgac cttactagta gtaaccccat ctttaggcaa agagtgaatg
    18001 gctctagtaa cctgtttaag acctaacgct tccgcaacat ctttcgccac tgcccaccat
    18061 tcatcattaa tatcgacaaa tcttataatg tgtccgttcc aaatttcagt tttcattcaa
    18121 cgtcctccag ttcaagccct agcaagctcg ctaacaactc aacttgctga tacaaaattt
    18181 cttgatactc ttcaatagaa agtcgttctt taaactctgc ttgtactaaa tcgttcaagc
    18241 cacgcaatac tccgcttacc gttggttctt gtggtagcaa tttttgatag tccatttgtt
    18301 cgtacaaaca ttcaaacgtt tcttctagcg ctttgccttc caagtttgct aattctgccg
    18361 ctgtgtcttc tgtaagaatt gttgataata ctttttcgat tgctttattt tgtgtcgatt
    18421 ccattattat ttcctcctat taatcccttt gcccccacgg cttgcgttta tttttggtat
    18481 tcttccataa agcgatcaca ttcatcttgt gaaattctta cgactccatc aactataatc
    18541 aactttaaac cgttaggaac atatttctgc atcatagtat tgtatgaaac gttcatatac
    18601 ttagcagctt gtgccttgtt catatatttt ggaaactggt actttttttc taataatgaa
    18661 agtaacgttc cttgagtttg ctctacttgt tgaacaactc gatcgtcaat aattttaaaa
    18721 gctggatcat caagcgtgag tttgtcaatt tttaattcca tatttatccc ccctcgttag
    18781 catacatttc tatacaaaga ttttaaaaaa atgcaagagg ctcttgaata catttctata
    18841 cctcttataa actcaatata ccatcatacc ttttcatttg caagaatttt aacatacttt
    18901 tttgaacttt tatgtttcac acatatttaa atcgtgatat agtagagaaa atatatgaat
    18961 ggtggtgaaa atgtggctag aaaaaaatac cccgaagcag tggagacagg acaaagaatt
    19021 agactggcta gaattgaaaa gggactctca atggaacaac ttggaggtaa attatcccca
    19081 ccggccagta aaggtgccgt tagtaattgg gaaaatggct ataatcttcc caataacacc
    19141 aggcttaaac aactttcaaa ggtattagat gtatccacta cctatctatt aaacggatct
    19201 tatacattaa gagatatgca tatgatgccc gaaattaagc gaaatgtgct tgaaaagcaa
    19261 ttgaaaaaaa ctattgaaaa aaataactct ataattgatt ctaatctttc taatgctatc
    19321 aataacatcg aagaagataa cctgactgtc tcacagaaga tttatttaac aaacgcaata
    19381 aaatatctta aaacaggatc tcgaattgaa tttcctgacg ggagtacccc aattgcttat
    19441 ctcgcagctc ttctaaatac gcttttacga tatgactcat taattaatga ttcagaaaaa
    19501 aatacagatg agaaaaaaga gtatcaagaa ttttatgaaa atgatcttct aaaagagttc
    19561 acagctcttg ttactgagct aaataaacac tattcaaaat aataataaac tgcctcctcc
    19621 aaccgccccc acggcttgct acactatgga gggaaaacta ttatggcaac attcaagcaa
    19681 tatgaaacta aaaaaggtaa attttggctc tatgaagcct atcttggaat gaacaaaatg
    19741 actggcaaac ctgataaggt tcgtagacga ggttacaaaa caaaaaaaga agcccagctt
    19801 gccttgtcac gcttgcaagt ggactacgat aaaaacggta ttaaaaaagc gaacaacgaa
    19861 acgtttaaag aagtttatga cttgtggcta gaaacatata aaaccactgt ccgagaagcc
    19921 accttcatga aaactgaaat caagtttaga aagtggattc ttccaaaata tggaaatctt
    19981 cgtgtgaatg aagtcacggt aaaaaaagcc caacaaattg taaatagctg ggctgaaact
    20041 actgaccaat acaaggtact tcattcaact tctaagcgta tttttagcta cgctatcaat
    20101 cttgggatta ttgagactaa tccactagac aacgtattaa tgcctaaaag aaccaccact
    20161 aagcataaag aaaaggtcaa agtttacagt aaagaacaac tgatcacctt gtttgattac
    20221 ctaaacagca aacctgaaac ttatcgaaac gactttgata aaacgttact acgctttctc
    20281 ttttatagcg gggttaggat cagtgaagcc cttgcattga attggtcaga cattgatttt
    20341 caagaaaata cggtcaccat caataaaaca ctaagccaat ccaaaaatgg ctataaaatc
    20401 agtgatccta aaactgattg tagtgctggg gttcttccac tagatgaaag aacaatttct
    20461 atattaaaaa aatggcagat taatcagcgg aaatatatgc tgacacttgg tgtaactgat
    20521 ccgactatga ttttttgtgg tatctataaa gaactaatca cacaccacgc tatttatgca
    20581 agattgatta ctatttgtga aaaagctatg gttccatttc tcggtgtgca tgtagcaaga
    20641 cacactcatg cttcaatgct cttggacgct ggagctacta tggtagaagt tcaaactaga
    20701 ttacggcata caaaaattag tcagacgatc gattgttacg gtcacttggc aaaagaaacg
    20761 aaagaaaaga ctgttgaaaa attagtacaa cacttaaata ttgactagat ggcatacaat
    20821 atggcataca gtaaaaaata aaaaaaggag aaaccctatc atatcaaggt ttctcctagc
    20881 tttatagtct attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
    20941 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
    21001 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
    21061 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
    21121 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
    21181 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
    21241 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
    21301 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
    21361 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
    21421 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
    21481 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
    21541 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
    21601 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
    21661 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
    21721 gaatcaaatt tcgagcatca cgataacgag gaatgtaggc cgggtctcca gaaagcaagt
    21781 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
    21841 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
    21901 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
    21961 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
    22021 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
    22081 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
    22141 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
    22201 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
    22261 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
    22321 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
    22381 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
    22441 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
    22501 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
    22561 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
    22621 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
    22681 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
    22741 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
    22801 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
    22861 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
    22921 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
    22981 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
    23041 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
    23101 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
    23161 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
    23221 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
    23281 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
    23341 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
    23401 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
    23461 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
    23521 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
    23581 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
    23641 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
    23701 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
    23761 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
    23821 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
    23881 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
    23941 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
    24001 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
    24061 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
    24121 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
    24181 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
    24241 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
    24301 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
    24361 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
    24421 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
    24481 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
    24541 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
    24601 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
    24661 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
    24721 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
    24781 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
    24841 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
    24901 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
    24961 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
    25021 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
    25081 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
    25141 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
    25201 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
    25261 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
    25321 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
    25381 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
    25441 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
    25501 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
    25561 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
    25621 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
    25681 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
    25741 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
    25801 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
    25861 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
    25921 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
    25981 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
    26041 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
    26101 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
    26161 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
    26221 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
    26281 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
    26341 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
    26401 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
    26461 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
    26521 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
    26581 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
    26641 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
    26701 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
    26761 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
    26821 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
    26881 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
    26941 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
    27001 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
    27061 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
    27121 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
    27181 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
    27241 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
    27301 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
    27361 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
    27421 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
    27481 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
    27541 ctctgctaat agatgtaatg ttttcagtat taat
//
LOCUS       DARDHU010000025        27073 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000025 DARDHU010000000
VERSION     DARDHU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.025"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            2..74
                     /locus_tag="SDD03_001158"
     tRNA            2..74
                     /locus_tag="SDD03_001158"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="SDD03_001159"
     tRNA            78..150
                     /locus_tag="SDD03_001159"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="SDD03_001160"
     tRNA            155..225
                     /locus_tag="SDD03_001160"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="SDD03_001161"
     tRNA            238..311
                     /locus_tag="SDD03_001161"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="SDD03_001162"
     tRNA            323..394
                     /locus_tag="SDD03_001162"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="SDD03_001163"
     CDS             660..1853
                     /locus_tag="SDD03_001163"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG4303617.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="SDD03_001164"
     CDS             1983..3455
                     /locus_tag="SDD03_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4303618.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="SDD03_001165"
     CDS             3538..4326
                     /locus_tag="SDD03_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG4303619.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="SDD03_001166"
     CDS             complement(4393..5061)
                     /locus_tag="SDD03_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG4303620.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="SDD03_001167"
     CDS             5176..6888
                     /locus_tag="SDD03_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303621.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="SDD03_001168"
     CDS             6891..8660
                     /locus_tag="SDD03_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303622.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="SDD03_001169"
     CDS             complement(8865..9431)
                     /locus_tag="SDD03_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4303623.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="SDD03_001170"
     CDS             complement(9431..10387)
                     /locus_tag="SDD03_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG4303624.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="SDD03_001171"
     CDS             complement(10387..11043)
                     /locus_tag="SDD03_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG4303625.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="SDD03_001172"
     CDS             11413..13431
                     /locus_tag="SDD03_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4303626.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /locus_tag="SDD03_001173"
     CDS             13858..16272
                     /locus_tag="SDD03_001173"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG4303627.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="SDD03_001174"
     CDS             16620..18173
                     /locus_tag="SDD03_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG4303628.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="SDD03_001175"
     CDS             complement(18554..20338)
                     /locus_tag="SDD03_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303629.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="SDD03_001176"
     CDS             complement(20353..22074)
                     /locus_tag="SDD03_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303630.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="SDD03_001177"
     CDS             22174..22935
                     /locus_tag="SDD03_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4303631.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23902..24170
                     /locus_tag="SDD03_001178"
                     /pseudo
     CDS             23902..24170
                     /locus_tag="SDD03_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24842..>27073
                     /locus_tag="SDD03_001179"
     CDS             24842..>27073
                     /locus_tag="SDD03_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303632.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         8647 a         4765 c         5655 g         8006 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat
    23401 actaccgata gcattttttt gcttataaaa taattataag tatttagaga tttttcttct
    23461 aacattcttc aaacaagaaa aatcacaaag gttattgtta tttttatata ttctttttaa
    23521 gaatgattgt ttttttatag aaattttaaa aaataaaaca cgttattgat ggatagttag
    23581 tttgttattt ggtaatatta tagaagttga ctggtagtga tttatttttc gtcgttttat
    23641 aaaagtgtta ttttttggaa aaattcacaa atatgtaacg aatgtttttc tggaatttgc
    23701 tgtgttaaaa agcagtatga ctaatataaa tactattgtc atttatgcaa ttttttatgg
    23761 atagtaattt tagaggggaa tcaatgaagg aagaatagaa gggaaaaata gttgcaaata
    23821 cgccagttag caaaaatagc aatacatctt caagctttaa aattcgagag aatcatcttg
    23881 gtagtggaat caactacaac aaaaggatta aaccaattaa tagatagaat taccaatgga
    23941 aaaatagaaa aggtagttat tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt
    24001 agtgaaaccc tttgcaatat atggtacaga atatagaaat tattgacatt acagaaaatg
    24061 atgaagattt aatacagatt gtcactattt gtagatacct tttacaagga aaccgagcca
    24121 ataaagctaa gaagatgatt aaggagttga tggaagacga tacaagttaa gaaagtgaaa
    24181 ctacgcccaa ataaagagca aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca
    24241 tcgattcggt ggacagttta ttagtaatga cggtttgaga acatccatga cgagaatgaa
    24301 ggaacatcct aaatgcgttt agttgaacca agtgccaaat aatatggcca aataagcgat
    24361 cagttatcga tgaatcccaa gtattctaaa ctatatattc attttgatgc taagcattga
    24421 tatatttttt tgggattgat gaaggttaac caatacaggg attcactgat gaaattctag
    24481 agtctgacct tggtatcaac gatttagtga ttgtttctaa tggaaaagta taaaagtata
    24541 taagtacatt aacaaaataa ctattagggt tacgctccag cttgtgcaag gcactggcag
    24601 gaggaatcgt gaaatcataa ttatggttaa gccacacgga caactaaaag tcatacaaag
    24661 ctatcgaatc aatcaacaga agacaatcgg caatcattag ctttgtttac agcttacatt
    24721 gcaacgcttg agttgaaccg aaaatacgag ggaggtaacg cagccatttt gagttgggga
    24781 atctttttag aaaattatcg ggtaccttgt tgtttagtca aaattataag ggggagtaat
    24841 agtgaggaac aaatttcata aactgttaag tatgtttcta gttatgctta ttgtgatcaa
    24901 tcaatttggg aacataacgg ttctagctga gtctttagca gaacagccag agcgttcgct
    24961 gacgatcgaa gttgatcaag aggaggctgc tttgggtgag cagttccagt tgcgagtcag
    25021 aggaacacca gacctgctaa atgcattggt cattgataag agtgaggatt tgattgaagg
    25081 ggaagtgaag caactcaatg attacgaacg tctgattact tattcttccc agcattcagg
    25141 taaatacgaa ttgaccgcaa gaagcggtga tattcaatca aatacggtct caatagaaat
    25201 cagtgtacca gaagaaagca caagtgagtc ttttaatgta ccagaagaaa gcacaagtga
    25261 atcttttaat ataccagaag aaagcttaag tgaatctttt aatgaagcca gatcatctga
    25321 gccatctata acgcaggaat ctcagattga aagacaagag gagctcttga attatgacat
    25381 cggcatctct gctgagcgcc ccactttgcc gagcaggcat acacaaacgt taaagggata
    25441 cattcaagga ggcgctggcg atattcttga agaatatggg gagatggtcg tacgagttcc
    25501 tagagcatcg attggcttaa ttgagttcat tagagtggag gtcgctgatg attcaccttt
    25561 aaagccactg gcattttacc aagaaggcac agacgttgtt tttccattta cctttgatta
    25621 tgaaggatta aatgaagccg ctcagtcgta tacattcagt atcacgtttg aagttgcttt
    25681 ctttcaatca ggcggtactg agccaaataa tgactttcaa gtgagtctta cttatgctga
    25741 gcgagcagca aacacggaat tttcaattac gcctattcct tcatcaagac ccattttttc
    25801 gaaattatac tatggctcta gagaaatcga cagccaagga aatcttggga atctgctgat
    25861 tgacgggcaa gacgtgacca acagtaataa atttattttg caggtaaatt atgaaggatc
    25921 acgaaaagac ggtgtagtgg ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa
    25981 tccgactaca tggagttcca ctggaaatag agaacctatt tcaggtgggg ttcggatcgc
    26041 agaagtaacg aatccaggtg aagcaaacgt tagaactcat tatgtttccg aggaatttgt
    26101 ggacaaagta tcttttaata aggaaacgaa tacacttgtt gtggactttg gtaatctagc
    26161 agagggtcaa tcgtatttta ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa
    26221 tgaaggaaga caagaggatg ggttttctcg gaatcgagcg agtatgacct acaataatca
    26281 ggtaatgagc cgagactacc gcttgagaat ccctgaaagt aatcttccgg caatctcttt
    26341 aagaaaaagt gtggacaaaa atgttatcaa tctcaatgaa aatgaactaa cgtatactct
    26401 gcatgtctcg atgttttctg gaacaagaaa tacgcttgtt attgaagatg ttttggaaga
    26461 aggcatggaa tttgtgtcat ttctatcaga tattccctct ttcattgatg ccgaagtcaa
    26521 taatggaata gttcgtttca gtgcgaataa tacgattggt ggtccgacaa gttttaccct
    26581 ctcttttaag gtcaatgtag aggcgtatgc ggtcggcgat caaattttaa actttgcaac
    26641 gcttgtgaat agtgacaatc gattgaatac gagtactgta agaacaagga aaatcgatgg
    26701 gcgtattaaa gtaattaaaa ctgatgagaa tggccaaagg ctggcaggag caaaatttga
    26761 aattttatca agcgattcac aagtgataca agaaggtata acggcagaaa acggagaatt
    26821 tttaagtgaa ccgcttcctg taggtagcta tcaagtccga gaaacagaag caccggaagg
    26881 gtatctttta gatgcgacgg tacatgatgt ttttataaat gaatactcca tagaagaagt
    26941 gacgttaacc attgaaaaca tccttgatac gggtggtgtt gaactaatta aacatgctga
    27001 aggcagtggt gaagtccttc aaggagctgt ttttgagtta cagaaccgag aaggagaaac
    27061 gctacaaacc ggt
//
LOCUS       DARDHU010000026        26284 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000026 DARDHU010000000
VERSION     DARDHU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.026"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(158..526)
                     /locus_tag="SDD03_001180"
     CDS             complement(158..526)
                     /locus_tag="SDD03_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG4303633.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            676..1158
                     /locus_tag="SDD03_001181"
     CDS             676..1158
                     /locus_tag="SDD03_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG4303634.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1195..2205
                     /locus_tag="SDD03_001182"
     CDS             1195..2205
                     /locus_tag="SDD03_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG4303635.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2320..3033)
                     /locus_tag="SDD03_001183"
     CDS             complement(2320..3033)
                     /locus_tag="SDD03_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303636.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3223..3813)
                     /locus_tag="SDD03_001184"
     CDS             complement(3223..3813)
                     /locus_tag="SDD03_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG4303637.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4297..4524
                     /locus_tag="SDD03_001185"
     CDS             4297..4524
                     /locus_tag="SDD03_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG4303638.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4540..5706
                     /locus_tag="SDD03_001186"
     CDS             4540..5706
                     /locus_tag="SDD03_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG4303639.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5940..6983
                     /gene="hrcA"
                     /locus_tag="SDD03_001187"
     CDS             5940..6983
                     /gene="hrcA"
                     /locus_tag="SDD03_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG4303640.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7077..7640
                     /gene="grpE"
                     /locus_tag="SDD03_001188"
     CDS             7077..7640
                     /gene="grpE"
                     /locus_tag="SDD03_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG4303641.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7691..9520
                     /gene="dnaK"
                     /locus_tag="SDD03_001189"
     CDS             7691..9520
                     /gene="dnaK"
                     /locus_tag="SDD03_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG4303642.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9671..10837
                     /gene="dnaJ"
                     /locus_tag="SDD03_001190"
     CDS             9671..10837
                     /gene="dnaJ"
                     /locus_tag="SDD03_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG4303643.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10919..11584)
                     /locus_tag="SDD03_001191"
     CDS             complement(10919..11584)
                     /locus_tag="SDD03_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303644.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11560..11733
                     /locus_tag="SDD03_001192"
     CDS             11560..11733
                     /locus_tag="SDD03_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303645.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11778..12515)
                     /locus_tag="SDD03_001193"
     CDS             complement(11778..12515)
                     /locus_tag="SDD03_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4303646.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12517..14019)
                     /locus_tag="SDD03_001194"
     CDS             complement(12517..14019)
                     /locus_tag="SDD03_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG4303647.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14278..16272
                     /gene="uvrB"
                     /locus_tag="SDD03_001195"
     CDS             14278..16272
                     /gene="uvrB"
                     /locus_tag="SDD03_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG4303648.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16282..19098
                     /gene="uvrA"
                     /locus_tag="SDD03_001196"
     CDS             16282..19098
                     /gene="uvrA"
                     /locus_tag="SDD03_001196"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG4303649.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19361..20626
                     /locus_tag="SDD03_001197"
     CDS             19361..20626
                     /locus_tag="SDD03_001197"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG4303650.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20630..21361
                     /locus_tag="SDD03_001198"
     CDS             20630..21361
                     /locus_tag="SDD03_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG4303651.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21496..22380
                     /gene="rapZ"
                     /locus_tag="SDD03_001199"
     CDS             21496..22380
                     /gene="rapZ"
                     /locus_tag="SDD03_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG4303652.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22377..23375
                     /locus_tag="SDD03_001200"
     CDS             22377..23375
                     /locus_tag="SDD03_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG4303653.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23409..24344
                     /gene="whiA"
                     /locus_tag="SDD03_001201"
     CDS             23409..24344
                     /gene="whiA"
                     /locus_tag="SDD03_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG4303654.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24887..25537
                     /locus_tag="SDD03_001202"
     CDS             24887..25537
                     /locus_tag="SDD03_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303655.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25724..>26284
                     /locus_tag="SDD03_001203"
     CDS             25724..>26284
                     /locus_tag="SDD03_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG4303656.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8676 a         4438 c         5590 g         7580 t
ORIGIN      
        1 gatttttttc agcacaagaa agactcctta taaaaatagc agatactttg tcaattaagt
       61 gtttaaccag ggatgataaa aaggatagga ttgaacaaaa cctgtataag gtgatgaata
      121 gtttcctcgc cagtaaaagg tttattcgta taaagcttta tcgccaaaat ttatcatgac
      181 cttttttatc ttcaatatta aatgaaatca attgttcaat caattcccag ttgattacac
      241 ttttccaagg aactttgatg acgttatccg tatatacgta gcctgcttct tggatatttt
      301 tttcaagggc acgaatcgct gctttttccg gtgcgataga aaaatgattt ttagcaaaac
      361 taaatgcaat aataaaggta ccatggtcag taaacatagg ttggttccat tttacagttg
      421 tgtctagctg gggatacgtt tctgttatct tagcaaaaag gtctttgatc ttttgttgat
      481 gttctttttc tttgatttga ttgatgatcg gctgaaattt ttccattcta aaacgcctcc
      541 attaagtggt ttgatttatt ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa
      601 acacaagtaa tgttgtaacc gctatattta tatgataaga tgtctcaaat aataaaatag
      661 aaaagagaca taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt
      721 gagttgtatc atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa
      781 caaggattta caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac
      841 tacgtatatc tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc
      901 ggtaaaaggc tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag
      961 ggcgctgttt ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc
     1021 ttatcaaatg aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa
     1081 acggttgact taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca
     1141 aacgaaaaaa acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata
     1201 agttatccat tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta
     1261 tcagaggaac accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc
     1321 caagtagtta ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga
     1381 aaacgagcag atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg
     1441 acttggggag gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa
     1501 ccaaaatgga tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa
     1561 acagggatag ccactgcaca tggaactaac cttattgatt taagaggaga gtacttggat
     1621 gaaacgacag caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag
     1681 caatcttctg aaaagtatca aaaagtgtgg ccccatgagc attcatcatc ttctgttttc
     1741 catctcactg aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga
     1801 cgattattag gtggttgtat tgatacgatc aggcatttaa tcggtactcc ttatggagat
     1861 gtccaatctt ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaac
     1921 tgtgagctat cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggctgg
     1981 tttgatggca gctcggggat catgttcggt agaagtgcgg cagaggattt gttagattat
     2041 aaaatgaagg atgtatacga agatctgtca aaggaattaa aactaccgat cttgtatgat
     2101 attgattgtg gtcatgtacc gccacaactg acattgatta atggtgccta tgcagaagta
     2161 gcggtagaag atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc
     2221 cttggagtct atcaaagacc aataaatcta aatagaaaag aaagtgaaag aagctgacaa
     2281 aatgctgtca gcttcttttt gtataacata ttagaaagtc taattatcta taactgtcac
     2341 atcgattaac tgtttcacgt cattgggatt tcctgtagga atagctacta cagatgcatt
     2401 atcaatgatt tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg
     2461 catttgccaa tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata
     2521 cactaaaact ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac
     2581 tgcactccct gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtaa
     2641 ttcgaaatca cccgatgtat ctctaaaagt attacctcta atagctagcc aaacacgtgc
     2701 gtactcaatt tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga
     2761 ggaggttgtg tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga
     2821 agaagtattg ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc
     2881 tgatttctga gtacttgatt tttcagctga actggtttgt ctgattgaac ttttgtctgt
     2941 ggtcgcagat ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga
     3001 gaccaacaat aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata
     3061 ccagtatagc ataatcctga atttttgaat ccgtaaaacc ttgtctatga atataaatag
     3121 agtgtatctt ctttgataaa tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac
     3181 ttgtcacact attgataagg agtatttaat tgaagagaat gattaggaga aaggatctcc
     3241 gtacttttta tagtaatcat agaatccaag tttgtataga cgaatcgttt tttctcttat
     3301 ttcttctgtt tcaaaatgga ctgcttgaag aatcaagtct gtgaattcta taggagctgt
     3361 tccattcgca gtaatcaaat tacgatcttt cacagcttgt tgttctataa aatcttcttt
     3421 attctgataa ttttccgatt ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc
     3481 ttgcaataat ccgctctgag ctaaataatc aactgcccca caaatagcag ctactggtac
     3541 gtctgttttt aacaatttat cgattatttg atataaatga tgatttttta tgtcccatga
     3601 attgccaccg atcataataa aaaggtcaac tttttcaggt atttgttcga ttaagtaatc
     3661 gatttttgtt tttaatcctc caatggaagt gacctctgat tggatagaag cagttttgtt
     3721 ttgccatttc tcattttggt ttagtgtgct agtaatataa gcaccttccc aatcggcata
     3781 ttcctctaat agaaacgtaa tcgcagtttt cattttcaag ctctccttta ctatctataa
     3841 caaggacaac agttttaacc tacagacatt cattaaaatt gggtagcgac agcgaaaaca
     3901 acaattctta agttttagac agagtataca gatagtcaac ttaattaaat acgaactaaa
     3961 taaaaatgac aggtaatcat ttttgtttac cagcattata acattttgca tacatggatg
     4021 acaaaagaac caattcccac ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa
     4081 gtaattatag aaacgaaaaa taaatcatat cgggcactca gacatgaaaa caaccctaga
     4141 gatatataac taggtaacca gtttaatgaa agaaaaaatc atccaagaag tcgattcttg
     4201 gattttttag tggatttgtt cgccaaatac gtttagtatg cgtatatgag aggttgtaag
     4261 caaagcgttg tggtctgagc acttaaggag gaatctgtga aaatagcttt tcatattttt
     4321 gcagattttg acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg agaggctgta
     4381 aaagcaccgt gtagctccga tgacaataag caagcatttt tagagctagg tttccttgat
     4441 tctaaatatg cggcttattt caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa
     4501 aacaggcacg acttttcgac gtaaggagaa agaatttcaa tgatagaaac aataaatccc
     4561 gcagaaaaca aggtttctgc gtacattcat atcccatttt gcgagcatat ctgctattac
     4621 tgtgatttta ataaagtttt tttagaaggg cagccagtag atgagtatgt ggaaatgctg
     4681 ctgaatgaaa tgaaaataat gctagaaagt catccgacag cgaatttaga aacactttac
     4741 gtagggggcg ggacaccaac ttctttatct gctaaacaac ttgatcgact gttatcaggt
     4801 gctagagaga tcctgccttt tcaaaatggt aatgagttta ctgtagaagc gaatccaggt
     4861 gatttgaccc gtgaaaaact tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg
     4921 ggagtgcaga catttgataa tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa
     4981 gatgtgtatc aaactatgca atttttagaa gcagaaaatt ttaccaatgt aagcatcgat
     5041 ttgatttatg cattaccggg acaaacacta gaaggatatc tggatacttt ggaaaaagct
     5101 ttggctttgg atctgcctca ctattctttg tactcattga tcctcgaaaa caaaacaatg
     5161 ttcatgaact gggtacgaca aggaagactc gaactgcctg atcaggaaac ggaaaccaga
     5221 atgtttgaag agacgattga agcaatggaa aagcatggac gtcaccaata tgaaataagt
     5281 aactttgggc tctccggaca tgaatctcaa cataacctca tgtactggaa caatgaccac
     5341 tattttgggt ttggagctgg tgctagcggt tatcttggag atattcgtta ccgcaacaaa
     5401 ggaccaatcc aacactattt acgcccatta cgagcaaatg aacgcccggt attggaagaa
     5461 gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa
     5521 acagggatta ctaaaaaaca tttctatgat cgctatcaaa aaacaattga atcggtgtac
     5581 ggtgatgtgc taagggaatt agagcaagaa gggttacttg ttaatgaaga agaccgtgtt
     5641 tatttgacac caagagggac ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa
     5701 aaatagttta aagaatattt gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac
     5761 aataaaggac tattgaaacg ttgatataac tggatttaca tcaattattt agaattagca
     5821 ctcttgtgtt tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg
     5881 ctatatttat aagtggatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga
     5941 tgttaacaaa gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg
     6001 gcgtgcccgt tggttccaaa acattgatgt ctgagggagt caaggccagc tcagcaacga
     6061 ttcgcaatga tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt
     6121 ctgggcggat tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg
     6181 ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg
     6241 aaatcaatga gattattaag caatctgctg agatattatc tgagctgaca agctatacga
     6301 ctttttcatt gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct
     6361 tgaataatcg acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag
     6421 tgttcactct tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa
     6481 acgaccgtct agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc
     6541 cgatgattct tcataaatat ttccaaacga cagaagggat gtctcagtta tttgacaccg
     6601 ttcttggtca tgtttttgaa gataaggttt atgtcagtgg acaaatgaat ctcttggatt
     6661 atgagccccg tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag
     6721 acggacttac acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg
     6781 aattaggcaa tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg
     6841 gtcatggaag aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt
     6901 tcggtctagt cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgtt
     6961 cgcttgactt gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct
     7021 ctttcttagt aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga
     7081 aagaaaatca agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg
     7141 atgtcgaagc tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag
     7201 cgaaattaaa agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa
     7261 tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac
     7321 aagatttagc caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag
     7381 aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt
     7441 tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg
     7501 atcctaatct tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata
     7561 cgattgtaga agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacca
     7621 tggtcattgt tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga
     7681 gacgttaatt atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc
     7741 agtattggaa ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc
     7801 ttctgttgta tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc
     7861 tgtgacaaac ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa
     7921 agtagatgta gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata
     7981 tatcaaagga ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt
     8041 gccagcttac tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg
     8101 tctagaagta gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga
     8161 taaaacagat cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt
     8221 atctatcctt gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca
     8281 tctaggtggg gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa
     8341 agaaaacagc attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc
     8401 tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat
     8461 cacagcagga gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga
     8521 tgaattaaca gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga
     8581 tgcaggttta tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat
     8641 ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa
     8701 ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt
     8761 gaaagatgtt gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg
     8821 agtgttcact aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt
     8881 ctcaactgcg gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc
     8941 aatggctgct gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc
     9001 tcgtggtatt ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt
     9061 atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg
     9121 tctaacagac gaagaaatcg aacgtatggt aaaagatgca gaagcaaatg cagaagcaga
     9181 taaagctcgt aaagaggaag ttgacctacg taatgatgta gatgcattgc tattctctgt
     9241 ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga
     9301 aacagctcgc gatgaattga aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac
     9361 taaacgcgat gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc
     9421 ggcacaacaa caagctcaag aaaacccaga agcagctcaa ggcggagcag atgatgtcgt
     9481 tgatgctgat tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag
     9541 agaaggccaa agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt
     9601 ttgtttcaaa caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg
     9661 aggaaagcct atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc
     9721 atcagaagat gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat
     9781 caataaagaa cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt
     9841 aagtgacccg caaaaacgcg ctgcatatga ccaatacggg catgcaggca cagatccaaa
     9901 ttacggtgct ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg
     9961 cttcggtggt tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga
    10021 tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga
    10081 agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg
    10141 tggtgggaac ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc
    10201 aggtacgatt aatgtagaac gccaaacacc acttggacga gtgatgagcc gtcaaacttg
    10261 tgatgtttgt catgggacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc
    10321 aggacatgag aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg
    10381 acaacaaatg cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga
    10441 tttgtatgtg gtcttccgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat
    10501 ttactatgaa ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc
    10561 aactgtccat ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt
    10621 ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt
    10681 caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc
    10741 attcgctgaa gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa
    10801 gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt
    10861 cattaataat taaaaaccag acgaacaatc atatgttcgc ctggtttttt tgtagttgtc
    10921 atgtttgtac caacaaaaaa gccagcttta gaatatgata atcgtcatag tttctagggg
    10981 tataaccagt gagagaatgg aacttgttca atcgatattg caaggtgttt ttatgaataa
    11041 ataattcttc tgagcaattt ttgatgcttc cgttattttt actgaagcat gccaaagtct
    11101 tcttgaacaa atgtacttct tcttctgtca ggcttttcaa aatacgtttt acatataatg
    11161 ttcgttgatc tgacgggatt gctggtaaaa cgatcccgag gtctaaatct tcaaaaaagc
    11221 ggatctcgtt cacagattga tggaaagcta tccattcgca aacttgtttg gcatgaagga
    11281 aagattgcgg tgtctcttgc tctgtataac aaagcgaccc aattccaaag tttagctgta
    11341 actgatacat tttattaaac tttgtctgga ttttatcaag caatgatagc aatgaggctc
    11401 tggaaatccg attgactagg ataatgattt gttgtctatg tacagtgagc aaagcctgtc
    11461 cgtaataaga gaagaccctt tcgagcaact ccaataaagg attgatccgt tcttgacaca
    11521 attgctgatt gtcagaaagt tgtcccacaa ctacataata tggagaagaa aaatcaagct
    11581 caattaagtg agggtggtat cgactatgta cttgatatca tcaatgaacc gatggtactt
    11641 tcttatgcga tggaacaggt agagcgattg cttgtttgtg caggaaaaca agcgatgcgg
    11701 ctgatacgat taggtgagaa aataagaaga taaaaataca cggtcccttc atggcgaccg
    11761 tgcatttttg gtttttatta aatatttaat actttattca agaaatcttt tgtccgttca
    11821 ttttgcggat tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca
    11881 atgaacatga cgcgattggc cacttcttta gcgaacccca tttcgtgcgt aacgataacc
    11941 atcgtcatac cttgttctgc taactttttc atgacgttca agacatcacc gaccatttca
    12001 ggatcgagtg cagaagtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca
    12061 cgagcaatag ccacacgctg tttttgtcca ccagaaagca tttcaggata catatctttt
    12121 ttatcttcta gaccaactgt ctgcagtaat ttgattgctt ttttctctgc ttcttctcta
    12181 gattctttct tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg
    12241 aataggttaa agtgttggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg
    12301 cttttatcca tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa
    12361 cgattcaagc aacgcaagaa tgtacttttt cctgaacctg aagggccaat gatacagacg
    12421 acatctcctt cgttgatgga tacattgatg tcgtttaata ctgtgttatc gccgtatttt
    12481 ttgactaaat gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac
    12541 ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta
    12601 aacatacgta ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac
    12661 tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa
    12721 tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata
    12781 agaaagacct aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc
    12841 tcgaacaatc tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag
    12901 gaagtcagga atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag
    12961 aaccatcatt ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc
    13021 gcgtattggt gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa
    13081 ggagatcaaa gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt
    13141 ttggataagt ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga
    13201 accatccgct acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt
    13261 caatccttca ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc
    13321 atattttccg ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc
    13381 ataaccgatt accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc
    13441 gtacaattga tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc
    13501 gcttaaaaat tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc
    13561 ttcataagac ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa
    13621 gtaagaatca gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat
    13681 cattccgtct gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc
    13741 cattcggatg ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc
    13801 ttcgtactcg ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat
    13861 tacgtatacg tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc
    13921 cgttgtagtg ttgtcagctt gtgcaacatg accaaacgca aatgtcacaa gtcctgtcat
    13981 taagaaaaca aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca
    14041 atattttatt attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat
    14101 gaaaaaaatg cgagtttcta acattttatg ttataaaaca taacatgtat aataagataa
    14161 aaaagtggcg aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt
    14221 ccttttcttt ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg
    14281 attgagagag atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat
    14341 cagcctgaag cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc
    14401 ttattaggtg cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg
    14461 aataaaccaa cactgattat tgcccataac aaaacattgg ctggtcagct gtacggggaa
    14521 ttcaaggaat ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat
    14581 cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat
    14641 gatgagatcg acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg
    14701 atcgtcattg catccgtttc atgtattttt ggattaggct cgccttttga gtaccaaaaa
    14761 caagtagttt cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta
    14821 gtcagtatcc aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc
    14881 ggtgatgtcg tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt
    14941 tttggagatg aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga
    15001 gaaacggaac acgtagcaat cttcccggcg actcactttg tgacgaatga tgaacatatg
    15061 gagcatgccg tagcgaatat caaagcggaa ttagaacagc gtttaactgt cttgcgaaat
    15121 gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg
    15181 atgttagaaa tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt
    15241 aaagaaggtg aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg
    15301 gcggacgaat cgcacgtaac catgcctcaa atcagaggaa tgtataatgg tgaccgagcg
    15361 agaaaacaga tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca
    15421 cttcggttag aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca
    15481 ggaccatatg aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg
    15541 ctgcttgacc cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc
    15601 gaaataaatg aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa
    15661 atggcagaag acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat
    15721 agtgatatca aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt
    15781 gatgtgttag ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt
    15841 gttgctatct tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa
    15901 acgattggac gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta
    15961 acagattcta tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat tcagcagaag
    16021 tacaatgaag aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt
    16081 tctattacga aagaatcgga agatgataca aaagaagcgg tacaagtctc gtacgaagaa
    16141 atgacgaagg aagaaaaaga cacgttattg atgaaattag aaaaagaaat gaaagatgcg
    16201 gcaaaagctt tggattttga aacagccgcg aatgtgcgag atatgatttt agaattaaaa
    16261 gcttcaaagt aggaggacaa catggctaac gataaaatta cgattcatgg tgcacgtgcc
    16321 cataacttga aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt
    16381 ttgtccggtt ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg
    16441 agatacgtag agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg
    16501 gacgtagata gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt
    16561 aaaaatcctc ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg
    16621 tttgcacgtg taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca
    16681 gtagatcaaa tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg
    16741 gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa
    16801 gagggatacg tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag
    16861 ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa
    16921 gggattcgtt ctcgcctatt tgattcttta gaggcggccc tgcgattagc agacggctat
    16981 gctctggttg acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct
    17041 tattgcggat ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt
    17101 ggagcttgtc cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg
    17161 attccagata gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc
    17221 tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat
    17281 acaccattcg aagaattacc ggaagagcat caggagatca tcttgaatgg gacagatgat
    17341 cttttccatt tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc
    17401 gaaggggtat tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt
    17461 gagcaaatgc gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg
    17521 aatccccaag ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg
    17581 tctattcaaa aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta
    17641 attgccaaac cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga
    17701 ctagactatc taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga
    17761 atcagattgg ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa
    17821 ccatcgatcg gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg
    17881 cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca
    17941 gattatttga ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca
    18001 ggcactcctg aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg
    18061 aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc
    18121 actggtgctt cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt
    18181 gtagcagtca caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag
    18241 aaatcgttag cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt
    18301 tccggctacg aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg
    18361 ccgagaagta atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct
    18421 caaacgaacg aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa
    18481 ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg
    18541 cctgatgtct atgttccttg tgaagtatgt catggtaaac gatataattc tgaaacatta
    18601 gaagtgcatt acaaaggaaa aagcattgct gatattttgg aaatgacagt agaggatgct
    18661 gtagaattct tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt
    18721 ggcttaggtt atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa
    18781 cggatgaaac ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta
    18841 gatgaaccaa cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa
    18901 agattagtag atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa
    18961 acagcagatt atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt
    19021 gcgactggaa caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg
    19081 aaaaaaataa tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat
    19141 gaatttttgc ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct
    19201 acactgattg gtatagataa aaacttccta taagttcaat ccgttcaaaa aagaattgag
    19261 caatcggagg actttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt
    19321 tggaaaagaa aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa
    19381 tgaagaattt atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc
    19441 ttttaatgaa gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac
    19501 ccgctacagt aaaattgtca atgtagaagt tattccagga tttgataaag accctgtatt
    19561 tatcgagacg atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct
    19621 tttgattgct tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc
    19681 agaatatttt atctgtccat atattgatta ttctttattt gaaaggctga tcaataaagt
    19741 aagcttttat gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag
    19801 agaagaaatg ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc
    19861 tttgaaacct gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc
    19921 ttttatttta gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg
    19981 ttataaaagt gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt
    20041 gctaaatgcg tatgtagatg aagatcatca agttcggatg atgtgccttg gtcatccact
    20101 attagaagat cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa
    20161 cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc
    20221 caatttcgat atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt
    20281 gagacaaggt cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgttttgt
    20341 cacagaagat cgagtgttca acaaaccctt tgttgaaacg acctatggaa cgaatcaatc
    20401 agataaagca agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga
    20461 aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt
    20521 attttatgaa aaagaccgat caatcaagcg ctggttgttg atgaaatata tgttccataa
    20581 ctatattcca agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt
    20641 tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa
    20701 ccaccgaacg aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc
    20761 aacagttcct gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt
    20821 tctgctggat gatattgaga aacaaaattt attgcgacca aacttcattg tgttgacttg
    20881 taacaccgca cactactttt ttgaagagtt acaagcggca acggatattc ctatcttgca
    20941 tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat
    21001 tctaggaaca gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg
    21061 tttcgaaaca gtgattcctg atactgcgct tcaagaaaaa atcaattacc tgatctatca
    21121 tgagatcaag gaatcggatt atttgaatca agagctctat tacgaaatct tagaggaagc
    21181 ggtagagcgc ctaaactgtg aaaaagtcat cctaggttgc acagaattat ctttgatgca
    21241 tgaatttgct gaagataatc attacccagt gatcgatgct caatctattt tagcggatcg
    21301 aacgatcgaa cgtgcattag ctgaaagaag cgaggcattg gatacagcat cggaaaagta
    21361 aaaaaattgg atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa
    21421 gaaaaagaaa actggtaatg atacgcccgg gatgatataa ttttattatg cacaatgtga
    21481 gaggggttcg taaatatgac agataatttg caattagtca tcataacagg tatgagtggt
    21541 gcagggaaaa cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat
    21601 atgccgcctc gattgattcc aaaattctgg gaattgatca aagaatcagg taaagtcaca
    21661 aaaatggctt taggagtgga tttgcgttct cgttccttct ttgaagaaat ccaaaacatg
    21721 ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca
    21781 gacgaagaac tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac
    21841 ggtttgatca ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa
    21901 gcttccgtga tcattgatac gacaacattg acaccacgac aattacgtga aaaaatcaat
    21961 gagtcattca aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt
    22021 aaatacgggt tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct
    22081 cattatatcc cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg
    22141 atgtcctttg atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta
    22201 ttgcctggat acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc
    22261 ggtcaacacc gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat
    22321 aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga
    22381 aaatgaaaac ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc
    22441 tgccagttat cctgaaaagt ttacgaaatc aaggagcaga tatcacagca gtagtgactg
    22501 ttgcagatga tggtggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg
    22561 gtgacttgag aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt
    22621 tccaataccg tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga
    22681 tcattgccgc agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa
    22741 aaatgatgca tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg
    22801 ctgtttttaa agacggaacg actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa
    22861 ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa
    22921 aagtcgtttc agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca
    22981 ccagtatcct tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg
    23041 ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag
    23101 atgccgatca tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat
    23161 tggtcaatac ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc
    23221 tagtacaagt cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca
    23281 ccgacttttt agaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag
    23341 aattgttccg tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg
    23401 agaataggat gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc
    23461 gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg
    23521 tgaaccagca gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct
    23581 attctctttt gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga
    23641 aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga
    23701 tggatcttga tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg
    23761 gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca
    23821 ataatccgga aacgagtcgt tatcatttgg aaatattttc tatttatgaa cagcataacc
    23881 aagacatttg cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca
    23941 atggctatat ttcgtattta aaaggagcag aaaaaattgc tgatttctta acattgattg
    24001 gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt
    24061 cggtcaatcg tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt
    24121 ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg
    24181 aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat
    24241 taggggaaat gattccctca ggtgcaatat caaaatcggg aattaatcac cgaattcgca
    24301 aaataaatga atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg
    24361 atacatgaag caggagatgc gaatgtatca gtttttttta aaagctatac ttgcttttct
    24421 tccgtcaaac tataattcca actttactgg agtaacataa tgttgaaagg cttctattgt
    24481 tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttatac
    24541 ataaactttt gcttaatata gaagtcctga tatagatagg atcaagtatt tatctataac
    24601 tggagattaa ttaaaaactg gaaggaactg ctcgatcaac cttgagaact gtttttctta
    24661 cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc attggattag
    24721 ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt
    24781 tattcagtga taatcataag ttcataaata gtaattggga tgtatggttg gtatacgtta
    24841 tacctgaact tgaactatca agatttggtt tagaatttga gaatcgatgt ttataaaggg
    24901 agggcaaaaa atgtgctttt ttacaacttt tactatcatt atgattgcag tactttttat
    24961 ttttctagat atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc
    25021 aggtctcata acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat
    25081 ttggagaact acagcaccac cattgccaga gattacatat ggagaatttc cgtttagtct
    25141 tgaatatgaa atagatgagg aaagaatttt gattgaagat acggtgattg cagaatttat
    25201 aggaagtttt agagggaatg tgacaactca acctagacga agttggagta taaaactttt
    25261 aaataatagt ggagagttga aagcatttca ttatgaattc ttaataatag aaagtgaaga
    25321 tgtaacaatc actttaagta gtggctctgc tggatatttt atgaatgatg gtggaagcaa
    25381 gccacagctc acagtccgtg atcgtattgc aggttttatg catttttctc actatgactt
    25441 agaagaagct cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt gggaatatgc
    25501 gccaccgata agaaattttt tcaatgatta ttattagtct tataaaaaac agaatgtaaa
    25561 atttatgtgc gcgagtattt agttttccat tttttgtttg atagatatat agcattgttg
    25621 aagcatggga tagtggtgcc aattttatca atgaggttgg tcaggcagtt aacataattg
    25681 gtgatgctat aaatccattt aattggaatt ggaggaatta ggtgtggaaa cagtgagatt
    25741 atggatattc atgaaaaatt ttcgttcggc tttgagatat gttattctgg aatcaaaatc
    25801 aggttattat ctgattgaca cagatcacta tactttttgg ggaggctacc tttttccgat
    25861 ttttaattgg ataatccccc ataaggttat gaaaattaac ttgcctgcag aagaaataaa
    25921 tagattaata ctcaacaaag atttacaaag gaaaagagag aaagaacatt ctaatgttgc
    25981 ttggataggt ccagcatttg cagtagtgac ttcttcaatt gtcggtccta tgctagagag
    26041 tatttatagt gatttttcta ttggaatacg ttatttattt gttactttaa gtatagctgt
    26101 tcttttgtgc ataaagggtt atgttagcaa gaagaataaa gatattttaa cactaatcgg
    26161 tgtaaacaat ttatctagta ctaaaattat actgtttcct gaatctataa aagaaggatc
    26221 aaggttgttg ataattttta taatgcttat actgttttct gttttatttg gggtgatgtt
    26281 tata
//
LOCUS       DARDHU010000027        25876 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000027 DARDHU010000000
VERSION     DARDHU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.027"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            157..2217
                     /locus_tag="SDD03_001204"
     CDS             157..2217
                     /locus_tag="SDD03_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG4303657.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2300..2869
                     /locus_tag="SDD03_001205"
     CDS             2300..2869
                     /locus_tag="SDD03_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4303658.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3044..3850)
                     /locus_tag="SDD03_001206"
     CDS             complement(3044..3850)
                     /locus_tag="SDD03_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303659.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3988..5502
                     /locus_tag="SDD03_001207"
     CDS             3988..5502
                     /locus_tag="SDD03_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG4303660.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5905..6888)
                     /locus_tag="SDD03_001208"
     CDS             complement(5905..6888)
                     /locus_tag="SDD03_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG4303661.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6893..7540)
                     /locus_tag="SDD03_001209"
     CDS             complement(6893..7540)
                     /locus_tag="SDD03_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4303662.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7625..8257)
                     /locus_tag="SDD03_001210"
     CDS             complement(7625..8257)
                     /locus_tag="SDD03_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4303663.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8273..8824)
                     /gene="coaC"
                     /locus_tag="SDD03_001211"
     CDS             complement(8273..8824)
                     /gene="coaC"
                     /locus_tag="SDD03_001211"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG4303664.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8821..9558)
                     /locus_tag="SDD03_001212"
     CDS             complement(8821..9558)
                     /locus_tag="SDD03_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG4303665.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9555..10544)
                     /locus_tag="SDD03_001213"
     CDS             complement(9555..10544)
                     /locus_tag="SDD03_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG4303666.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10802..11344
                     /locus_tag="SDD03_001214"
     CDS             10802..11344
                     /locus_tag="SDD03_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4303667.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11361..12446
                     /locus_tag="SDD03_001215"
     CDS             11361..12446
                     /locus_tag="SDD03_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303668.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12458..13264
                     /locus_tag="SDD03_001216"
     CDS             12458..13264
                     /locus_tag="SDD03_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303669.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13265..14092
                     /locus_tag="SDD03_001217"
     CDS             13265..14092
                     /locus_tag="SDD03_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303670.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14089..15162
                     /locus_tag="SDD03_001218"
     CDS             14089..15162
                     /locus_tag="SDD03_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4303671.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15590..18286
                     /locus_tag="SDD03_001219"
     CDS             15590..18286
                     /locus_tag="SDD03_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4303672.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18363..18851
                     /locus_tag="SDD03_001220"
     CDS             18363..18851
                     /locus_tag="SDD03_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4303673.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18863..19357
                     /locus_tag="SDD03_001221"
     CDS             18863..19357
                     /locus_tag="SDD03_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4303674.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19378..20187
                     /locus_tag="SDD03_001222"
     CDS             19378..20187
                     /locus_tag="SDD03_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4303675.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20184..21005
                     /locus_tag="SDD03_001223"
     CDS             20184..21005
                     /locus_tag="SDD03_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4303676.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21056..23155
                     /locus_tag="SDD03_001224"
     CDS             21056..23155
                     /locus_tag="SDD03_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG4303677.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23152..24270
                     /locus_tag="SDD03_001225"
     CDS             23152..24270
                     /locus_tag="SDD03_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG4303678.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24309..25187
                     /locus_tag="SDD03_001226"
     CDS             24309..25187
                     /locus_tag="SDD03_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4303679.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25333..25530
                     /locus_tag="SDD03_001227"
     CDS             25333..25530
                     /locus_tag="SDD03_001227"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG4303680.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8189 a         4484 c         5449 g         7754 t
ORIGIN      
        1 acataagtta ttattccgat tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta
       61 atagtccatc ttcttttgat tttatttata taatgaaatc atcttatgaa aattactact
      121 tcatgtttag acaagagtag ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa
      181 caactaaata aacacgcttg tcgtttagat aaggaacggt cgtgttctga aaaacactac
      241 gttaagttta tgatgcgcac gatgctgatc agtttatctg taggtgtcta tatgcatgta
      301 cccgttcagg caacgacaca aaccatcaag gtcgtacgta cctctgtttc tgagaccact
      361 gattccccta tcactattac tcatactttc actgatgcaa atggaaaaca gctacttcct
      421 gcattgactt cctataataa agtcagttat cctccaacga taaaccacta ctggttaatt
      481 cctaacaaaa cattatggac atcggaaacc ggaagcagtg tgccgctttc tatcttttcc
      541 gatatgactg atcctacaaa aggtgatcag ttaaaacaga tcactgatat tttgaatcat
      601 gctttgacag atataaagga cggtggaagc gcacaagttt cttatgtata tgaaaaagat
      661 tattcacagt ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt ttcgatatct
      721 gatctggtag aaaggctgaa gaacgcagat ggaacacaag gagatatgtc caaagtaatt
      781 tcaaacaatg ggaatatcgt ggatacgact aatacaggaa catctactat ttccttgtcc
      841 tattttgatc caattgcttt aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct
      901 gatcaaacag ctatcaaagg aaaagatctt cacttaagaa caggagagag ttacgcccct
      961 aaagatcttg ttgaatatgt aacaaaaagc gatggaactt caggaaataa agaagaaatc
     1021 aaaatgaacg ggacagtaga tactacaact ccgggagaat atcccgttgt attaacttat
     1081 atagactcga aaacatcaaa tgtggcagaa acaacagctg tagtttatgt agaagcatct
     1141 gctgcttcat cgactgtcat cactcatttt atagatagtc aaacaaataa agaacttcaa
     1201 gcagaaacac atatgagtag tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt
     1261 tctgatcgag tgccaaatat agatttatcg acttatgaac aaaatgggaa agtcacttct
     1321 atttctgatt ggatgcaagc aaacggatta gacagaaaaa gtcactggtc ggagatcctt
     1381 gaagtacttc actcagaact gatgaataat aggaagcaat caggagggga aattaccttt
     1441 acttatgttt atataccaga caatcgttca ttagaaggtc atgatcttat tgttcatcca
     1501 aacgaaaaaa taaacaaaga agagttgatc caatcagcaa caaattcctt aggtaaaccc
     1561 gtaccgttaa aggatgtatc ggttacaatc aatgaagtac cattggagga tggttatttg
     1621 accaaacaaa caggtgattt tacgattatg tatagttatc atgacccgta ttcactccaa
     1681 gaactccatg ctcagtcact attacatgtt ttaccattaa acaaacagat agatcaaaaa
     1741 gaacaaaaaa gacgagaaga aagcttgtca gaaaaaaaac aacaaatggc tcatgcagtt
     1801 tctccaacag ggaaagtaaa acaaacaaga agcctagagt tagcaagaat atctagaaaa
     1861 atctcagaat ctaataaaac gataaggcaa gaagctcatt tggccccaca agtttctgga
     1921 ttggaaaatg aaattttaga gcctaaaata aaacaacaat cagaaaaaga atttgttgct
     1981 aaacagtcgg aagatcagaa ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg
     2041 tcaactattg attccaccga tgagcatctg cagccgcaag taccacttcc tagtccggga
     2101 acacccgcac ctggtggagg tattccttct tctgctttag gagatttcat gcgaggtatg
     2161 gcaggaacat gggtatacgc taatcgaaat gaatttgacg cagatgactg gttctgattt
     2221 atatctctat ctgttatagt caaataagta gctgagtcat atgttatttg gcataatgga
     2281 gacgaggagg gatagaaaaa tgttagataa aacgttacct tacgcagaaa tctggatgac
     2341 aagaccactt caacaaaatg ttccaatcac gcctttagcg gaaggttttg aatttcgatt
     2401 ttatcaaaaa gaagatgaat ctgcttgggc ggagattgag acttccgttg gggaatttca
     2461 aacgacagat gaagcacttg agtattttca acaaaacttt gctccttatc ctgaagaact
     2521 tgaaagaagg atgttttttg tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc
     2581 ttggcgaaaa aaatcaagag agcaagtgta tccggttttt cattggttag cagtgaaacc
     2641 agattaccaa ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc aagaatttcc
     2701 taccctccat acggacggac caatctatct tcacacacag acatggagcc atcaggcgat
     2761 tgctctttat aaaaaaatgg gttttacttt tattgctgaa aatctagacg gcacacctaa
     2821 tccagattat gaaaaagtta tgcaggtttt aagtaaatta gataaataac gaaatggtga
     2881 aataagtcct cagtattggg ataaaaaacg aaatagtcat tcctcatatc ttactttttt
     2941 gagttaatac tatcggataa ctaatggcat gccatacatt caaaaaagaa ggggcagtga
     3001 caaaagtatt gtcactgccc cttctcttgt tttttagcat gttttatcgt ttgatttttt
     3061 taggaataaa attcttgatg aacgtaaatt tttggtaatc gtttcgacta tagaggtaac
     3121 caaatacact gaaaacaact gctccaataa aattaacaaa taaatccttc atagtatcca
     3181 tgattcccac gtctaagtaa ccattttgga tgattgtttt atctgtttta ccatctgcat
     3241 tttgactttc aatgattgtc cgagtgatat ctggaacatg atagacagtg tttccgtctt
     3301 tacttaactc cacactattg atttgctgga tgatcgtatc tttttgcata tcgaattgca
     3361 gtaatttatc tgctgaaaat tccacaaact cccacataac accaactgtc atcgaaaaac
     3421 agaaggaaac aactgctagt aataaaggag tcaagttcat ggtttttgta tttttattga
     3481 gcaaataaac aagggaaaag ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa
     3541 aagtatccca cattgaaaaa tagccataaa aatcacttaa ctctcctaaa actgttgaac
     3601 taaaaataaa agataacaaa atgatttcta gcatatttgg aatctgtatc tttaatatct
     3661 tttccagaaa ataagggatt tcaaataaaa ttaatgtcag taataaaagc attgtatttc
     3721 cccagtgttg gttccagacc tgatccacta gcataataac gacgatgata cgtaataaaa
     3781 tatagattcc gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa aaggttttca
     3841 aatgattcat aaaataactc cttcatgtat atccttaatt atactaagaa gcgtgtgttt
     3901 accaaagcta tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa tcacttaata
     3961 tagtataagg agaaaagggg gatacaaatg aaaaaattag ttgctggttt attaacagcc
     4021 gttgcacttc ttgctatgag cgcatgcggc gcagatcaga aaaatacagc gcagacaagt
     4081 tctagtacga aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt atatacagat
     4141 ccaaaagaaa tgaaagaatc ttatgatgtc gtgattattg gagctggtgg tgcagggatg
     4201 gcagcagcta tcgaagcaaa agatgctggg ttgaatccag taattttgga aaagatgcct
     4261 gttgccggag gaaacacatc taaatcatca agcggtatga atgcttcaga aacaaaattt
     4321 caaaaagaag aaggaatcaa agactcgaat gatgccttct atgaagaaac gctaaaaggt
     4381 ggtggaggaa cgaatgataa agaaatgctt cgatactttg tcgatcactc tgctgatgcg
     4441 gtcgattggc tagatcaaaa cggtatcaaa ctggacaact taacgatcac tggagggatg
     4501 agtgaaaaga ggacacatcg tccgtctgat ggctcggcta ttggtggata cttagtggag
     4561 gggttattga ataacgtcca tgagcgggaa atccctattt ttgtcaacgc agatgtgaca
     4621 gacattaaga aaaatgggga aacggtcaat gaagtgactg tacatgttca aggagaagaa
     4681 ccaaaagagg tcaccggaaa agcaatcgta gttaccacag gcggttttgg tgccagcaaa
     4741 gaatttattg aaaaatatcg tcccgattta gctgattata ttacgacaaa tcaagaagga
     4801 tcaactggag atggtatcac catgattgaa aaattaggtg gacaaaccgt tgatatggat
     4861 aaaatacaaa ttcatccaac tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt
     4921 cgcggagaag gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat
     4981 acaagggata aagtatctgc agcaattaca gcattgcctg aaaaatcagc ttatttgatt
     5041 tttgaccaag gcgtgcgtga tcgtgccaca gctattgaat tttatgacca aaaaggatat
     5101 gtcacaactg gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca
     5161 ctagcaaaaa cagttgatgg atggaatacg gatgttgcgg agaaaaaaga tgctcaattc
     5221 aaccgtgcca cagccatgga acatgactta tcgtctccga attattatgc gatcaaaatc
     5281 gcaccaggaa tccattacac gatgggcggt gtgaaaatca atacgaacac tgaagtatta
     5341 gataaagaaa accagccaat tagggggtta tatgctgctg gtgaagtcac aggtggctta
     5401 catggaaaca accggatcgg tggaaattca gttgcggaga tcattatttt tgggcgacaa
     5461 gcgggcatac aagcagctcg atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa
     5521 agaggttgtg aaatcagcgt tttgagctaa taccgagggt cagctgattg aacactgttt
     5581 agtgaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaac
     5641 agcttcttat gtttttaact tggttggact tattaccgca gatgcaagct tttgaacacc
     5701 gtttattcgg aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     5761 cacgtataaa cggcggaagc agaagaaatt ccttcggaaa taagctgaaa ttcacaaaaa
     5821 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
     5881 gcctcttttt tgatcagcat gattttattc agttatcttc aaaatgattt ttccgatgtt
     5941 tttgtttgct tccatatatt catgcgcttg tttgactgcg ctgaattgga atgtacggtc
     6001 aatgattggc cgaatcttgc cattttctag aaaaggccca gctttttgca taaaagtttg
     6061 tgcgagctct gctttatact gatcacttct aggtgtcaat aatgtaccaa gcagttggac
     6121 acgtttccca atcaatacac caaggtctat ctcaggcacg atggtcccac ctagcatacc
     6181 aatcaagacc caacgtccat caacggctat agaagctaaa ttcttttccc agtaagaagc
     6241 gccgatgaaa tccaaaataa catcgactcc ccgaccttca gtgatttttc gtacctcttc
     6301 ttgaaagtct tgttttttat agttgatcaa ttcatctgca ccaagttctt tacaaaaggc
     6361 gagtttttct tctgatcctg cagtaacgat gattttcgcg tgagaaagct gtctagctaa
     6421 ttggatcgct gctgttccca caccacttgc tcctgcatgg atcaagacag tttcgccttc
     6481 ttgtaatttt ccaagccagt aaagtgtttg ataagcagtc aaaaaaactt ctgagattcc
     6541 agcagcagaa acatagtcta gcgtatcagg gagaaggatt gcacgatcgg caggcatcac
     6601 agcatattcg gcataaccac ctagattgac taatccgtat acttttgtcc caggagtaaa
     6661 agtggagata tcacttttat tctcaactac ctctccagct acttcgactc ccaaaatagg
     6721 gtagggaggt ttcaaagaga ctgattctct tttcataata tctgttcgat tgacagctgc
     6781 tgtatggact ttgatcagta attcgccttg ttttggttta ggaacaggca cttcaatttg
     6841 ttctaataca tctacagaac caggatgctt gattgaaatg gcacgcatgg atctattcct
     6901 cctttgccca ttcttggaat gctgtattca actctttgct accttcagaa agtactagag
     6961 tcgtacgaga aatatccgtt tcttccacta gagcggcaag tacagcagca actgtagagc
     7021 gagtgatcgt ttttgatgcc ttctcattag gcttagttaa ttgaatggaa gtaacttctt
     7081 catcgttagt caatgtgact ggttgaagaa tgacaaaatc taagttagtt cgtttcagcc
     7141 attcatctgc ataatgttta gtaatataat aatctgtcat ggactcaggc cattttgctg
     7201 gttcatctgc ataaacagca ctgaccatca gatagcgaga gacatgcgca ttttcagctg
     7261 cgatcactgt tttgattgcg ccatccaagt ctacttgcag aagattcttt ccttgagagc
     7321 cggcagcaaa aatcaataca tcgatgcctt tgaatgcttc ggtcattttt tccacggacc
     7381 aagttaaatc aaaagagaca tacgaaacat tctctttttt tactaatgtt tgggaagcaa
     7441 catctctcac ccctgcaaaa atttcatggg aacttggagc taaatctttt atcaggtgtc
     7501 ggccaatttg tccgtttgca cctacaacaa aaattttcat ttggatcatc ctttcgacgt
     7561 caaatctttc cattcaatag taacaccaaa agagaaaaaa atcagagatc ggctacgatg
     7621 actatcagtt agtaagagaa gggtgcttga agtatcttaa caagacattg catactgctg
     7681 cagtcgcaag acctgcgacg attccttcag gtataccact tgtaaaaata accgctagaa
     7741 tagctgaata aacagcattt aaatttgttc cgatgacttt ggcataatct tgctggaata
     7801 aaaagtagat catattcatc actaaaatcg tattggtcaa tgaaccggaa agtccagcta
     7861 agaataaaga aacggaatct gctttttgtt tcattagttt gcgcagacct ttataaacga
     7921 aataaggaac caccccaatc aagatgcgag gaataaacgc gatcagcaag gctttccagc
     7981 ttccatgttc tgttccgatg actgggataa acggagaaaa gacaaaagac aaaggtgttt
     8041 ggataactgt gctacggatc atgctgatca gtccaaatgt accgccaaga aaagcaccta
     8101 gtcttggtcc taaaacgatc gaagcaatga ttacaggaat atgcatggtt gttgcattga
     8161 ttggaccgat agggataaaa cctaaaggcg taacagctaa taaaatcaaa atagctaaaa
     8221 acatcgcagt taatgtaaag ttttttgtat ttttcataag tactcctttt ttctaagcat
     8281 ttctttcatt aagagtcagc atgacctgtt cgatgatcgt ctgaatatca gctaaggcac
     8341 cttttccaaa atctccacaa gctaaaagag attctcttgg ttcaatttcc tgatagccga
     8401 cagatctcaa tgtttccaga ttacgctgag tgatcgggtt ttgatacata ttcgtgttca
     8461 tggcaggggc aatcaatttt ggtgttccca ctggaagcgc cattgcgact gttgaaagaa
     8521 gatcgtctgc aatgccgttt gctagcttgc cgataatgtt tgctgtagca ggcgcgatga
     8581 gaaacaagtc agcttgtttt gctagttcga tatgattgat tacctgcgga tttgattctt
     8641 gcatgacgtc tgtatggact gcgcgcttcg acagagattg caaggttaat ggcgtgataa
     8701 aatgtgtgct gcttttagac ataatcacat ctacctgggc acctaatttg acgaattgac
     8761 ttgtaagatc agcgcttttg taagcagaaa tacttccagt aacgcctagt aagatctttt
     8821 tcattttgat tcctccagtc taattttctc agtaatcaat gcagcaatct cagatttact
     8881 ttgagcttct tccactgttc catctttaga taagagatag ccgtggtgtg tctcgccatg
     8941 gatttccgtc aaatcatttg cgaaaataaa atcagttttg ttatttatca gactattttt
     9001 tgcgactgcc agaagagatt cctttggtac atctactaat agtttgaaac caatgatttt
     9061 tgcttttggc tgccattcac gtagataaga aatgattttt ggtgttttct tcaaaacgag
     9121 cacaagatgc tcggtgttag aagagatctt tgaaacagtg gtatctgtct gttcattttc
     9181 cgcaaaccaa ttgtccagtt cttcttgcgt tgaagaagtg ggtaggtttt ttgccagctg
     9241 ttcttctgaa aaactaaaag caggagtaaa atcactgact gccatactgt ggataatcgc
     9301 atcgtagtgt tcttttttcg ttagttcttc taattgtaaa aataagtcca gtgcagattc
     9361 gatcgtataa atcgttatat tggagtgtct tttcggtttc agtgcttgtt tcgtagtcac
     9421 gtaatcgatt gtggtatccg gattggcagc taaataatca gctaatgctt gccctaatcg
     9481 tcctgtcgaa tgatttgtaa tagcacgtac ttgatcgatt ttttcagtcg ttcctccagc
     9541 tgtgattaga atcttcattt atggtcacct acatttacta gtgttcttcc ttggtgtgtt
     9601 ccagcaagta gtttagatgc tgtttcaggc aattgatcca gagtaatctc ttggacgacc
     9661 agttcatctg caatctgcaa atccgccatc tgttgccata aagaaagacg ttgatctatt
     9721 ggtacattga cagaatcaat tccaatcaac tggatgtttc gcagaataaa aggcaagaca
     9781 gtcgcgttta gtttgattcc cccagcattg ccacataaag ctactgcacc gttataagag
     9841 atcaatggaa gaagactgct tagctgctcg ccacctactg tgtcgatgac atagtcgatt
     9901 tgctgttttc ctaaagcttt tgttgtctct ggcagaaatt catcaggtcg tacgacttgc
     9961 aaagcaccta gactttttag ccatgtgaca gactcttctt ttctggaaaa ggcaatgatt
    10021 gactggaatc ctagtttatg taacagggct atcgctgtac tgcctacacc gccagaagca
    10081 ccagtaacga caactttttt atcggaggtc ataccttgac ggattaaagc gttcacacac
    10141 aacatagcag taaaccctgc tgtaccaagg atcatcgctt gacgcagcgt catatttttt
    10201 ggtagaggaa ctaaccagtc tcctggtact ttttgatatt ggctataacc gcctggatgg
    10261 gtgacgccaa gcccaaaacc agtagcaagt acaagatctt cttttgaaaa acgtggattg
    10321 ttactttgga cgacagttcc ggataaatca atccctggag tcatcggata tgttcgtatc
    10381 acctgaccag actcgctcat tgccaatgca tctttataat tgacatcaga ataggcaatt
    10441 tttaccaaga cttcattttc agccaactca gtaaatggca gatcactgat tgctgtatgg
    10501 aaaacaggtt cctttattac ctgaaaagat cgaaaagcgt tcattcgtga gacactcctt
    10561 ttaaaatttt cttttatcat accataaagc ctactctttt attcttgttc ttccgtaaat
    10621 ttagacaaaa attaggtgaa aaataaagaa tcaaacaact agaatttatt ccaaaagata
    10681 aagaaatatt cagcaaaaaa aattttacaa agggttgcct ttttcaataa atttcgtata
    10741 ctaatacgag tggtagtaaa caggaagttt actattagaa aacttcgatt cggaggaaat
    10801 tatggaaatt ggtgagaagt taagaaattt gcgcatccaa aagaatttga cacaagaaga
    10861 actaggcgaa cgcacagatt tgtcaaaagg gtatatttcc cagttggaac gagatttaag
    10921 ttcaccatca atggaaacat tcttttctat tcttgaagtc ttaggtgtga ctccagaaga
    10981 attttttcat caagaagcag cgaatctgca agtggtttat tcaaaagaag atcatacggt
    11041 ttatttcgat gaagaaaacg ggtatgaatt ggaatggctc gtggctgatt cgaatgaaaa
    11101 agaaatggag cctgtcttgc tgacttttga aggtgcagga gaatataaga cgtttgaacc
    11161 ttcattatct gaaacgttta tttttgtatt agaaggggaa ttagagttaa gcttaggaga
    11221 gacggtctat gtggcaaaag caggacagtc gatctattat caagcgacaa aacaacatca
    11281 attaaggaat catctcaaaa aacgaacaaa agtattgatt gttgcaacgc aatcatattt
    11341 gtaatatagg gggtaaaaac gtggggaaac caatcatttc atttgatcaa gtagtgaaac
    11401 gctatgatga tgaaacagta ttaaaaaaag taagttttga gatcgaacaa ggaaaatttt
    11461 atacactgct tggtccttcc ggctgtggta aaacaacgat cctacgtatt atagctggat
    11521 tcaccgaagc tacagaaggg gacatctatt ttgaagggaa acggttgaat gatttgccag
    11581 ctaataagcg acaagtaaat acagtcttcc aagattatgc actttttcct catatgaatg
    11641 tttttgataa cgttgctttt ggattgaaga tcaagaaaat gtccaaatca gatatcgaaa
    11701 agaaggtaaa agatgctttg cgtatggtcc agcttcctgg atacgaaaaa cgggagatta
    11761 gtgaaatgtc tggtggtcaa agacagcgtg tagcgattgc acgagcaatt gtaaatgaac
    11821 cgaaagtatt gctattagat gaaccgttat cggcgcttga tttgaaacta cgtacagata
    11881 tgcagtatga actgcgggat ctgcaacaac gattaggaat cacgtttatt tttgttactc
    11941 atgaccaaga agaagcgttg gcaatgagtg atgagatttt tgtcatgaat aaagggaaaa
    12001 tcgtccaaag tggtacaccc gtagatattt atgatgagcc aatcaatcat ttcgttgctg
    12061 attttgttgg ggagagcaat atcgtggacg gtgtaatgat cgaagataat ctagttgagt
    12121 ttgttggtaa gcaatttgaa tgtgtcgatg gcgggatgcg tctaaatgaa ccagttgaag
    12181 ttgttttacg cccagaggat ttgacgatca cgacaccaga taaaggaaaa ctagtcgtaa
    12241 cggtcgatac tcagctgttt cgtggtgtcc attatgaaat catctgttat gatgaacagc
    12301 aaaatgaatg gatggtccat tcaacgaaaa aagctaaaga aggaagcaaa gtaggattgg
    12361 cctttgagcc agaagatatc catgtcatgc gtttcaacga gtctgaagaa gaattcgatg
    12421 cacgtcttga cagctacgaa gaataggggg gacaagaatg aaaacaatgc gcaggatcta
    12481 ttccgtccct tatattttgt ggctcggttt attcgtgatc gcgcctgtat taatgatcat
    12541 ttatcaatct ttttttgata tgaatgggca attcacgctg aataattatc tatcttattt
    12601 tacgtcgggg acatcgcttt ctatgacgat caattctgta tggtatgcat ttttgatcac
    12661 gtttttcacg ctgatcatca gttacccgac tgcttatttt ttaacaaaac tgaagcacaa
    12721 gcaattatgg ctgatgctga ttattttgcc tacatgggtg aatctgctgc ttaaagctta
    12781 cgcatttatt gggattttca gtgtgcacgg cagcgtgaat catttctttg attttatcgg
    12841 gatcgggccg aaacagatat tattcacaga cttcagtttt ctgttcgtgg caacgtatat
    12901 cgagattcca tttatgatta tgccaatttt caatgcattg gaagagatga atccttcttt
    12961 gatcagtgct agtagagatt taggcgcgaa tagctttgag accttccgac gagtgatttt
    13021 tccgttatta ttaaatggtg tgaaaagtgg tgtccaagca gtatttattc cttctctttc
    13081 tttattcatg ctgactcgtc tgattggcgg aaatcgggtg atcactttag gaacggctat
    13141 cgaacagcat ttcttggtta cacaaaactg ggggatgggt tctacaatcg gtgtgatttt
    13201 aatcattgct atgttcatcg tgatgctcct gacaggcgag aagaaaaaag gaggtcgctc
    13261 ataaatgaaa aaatttaaat ggtcgtatct ttatttaggg atcgtctttt tattcttata
    13321 tgcaccgatc ttctatctga ttttttattc cttcaatgaa ggaggaacga tgaacagctt
    13381 tacaggattt acttgggaaa attatgaagc tgtttttgaa gatacacgtt tgatcattat
    13441 cgtattgaat acgttcatgc tggctttctt atctgcattg atcgccacga tcatcgggac
    13501 atttggggct atgggaatct attacacgaa aaaacgacgt gcacggacag cgttgttgag
    13561 catgaataac atcttattag tctctcctga tgttattatc ggtgctagtt tcttgatttt
    13621 ctttacacta ctaggattcg gattaggttt tcagagtgta ttgctttccc atattgcttt
    13681 cagcattcca atcgtcgtct tgatggtctt gccaaaattg caggaaatga acgattcaat
    13741 ggtcgatgcg gctagagacc ttggtgctaa taatttccaa gtagtcaaaa atatcatttt
    13801 gccttatctt tctccaggaa tcatagctgg atattttatg gcatttacct attctcttga
    13861 tgattttgcg gttacatttt tcgtcacagg aaatggcttt acgacgctct cagtagagat
    13921 ctattctcgt gcaagacaag gaatcagttt agaaatcaat gcgctaagtg cgttagtttt
    13981 cttgttctcc atgatattgg tcattggata ctactttatt agtaaagaaa accgtccaaa
    14041 acggctgaag aaaaacagaa gagtacgcga gggggtggca cggctgcgat gaaaaaatta
    14101 caatctctgt ttttaggtat tctcatcatt attgcggtgc tgctgcttgg tgttcgtcaa
    14161 cttgaaaaaa caagtggcat gagtggagca aaggtactta ccatctataa ctggggagat
    14221 tatatcgatc ctagtttgat cacgaaattt gaaaaagaat atggatacaa agtgaattat
    14281 gaaacatttg attctaatga agcgatgttc acaaaaatcc agcaaggtgg gacaaactac
    14341 gacatcgcta taccaagtga atatatgatc caaaaaatga tcaaagaaaa actagtactt
    14401 ccattagatc attcgaagat caaaggatta aataatattg atccacgatt tttagacttg
    14461 gattttgatc cagataatca gtattcgatt ccatattttt ggggaacttt agggatcgta
    14521 tataacgata aattctttga tgcaaatcaa attaagcatt gggatgattt gtggaagccg
    14581 gaattaaaag acagtttgat gctgattgac ggtgcacgag aagttatggg gctttctttg
    14641 aatagtttag gctattcttt gaatagtaag aatatggcac agttgacaga agcagcaaat
    14701 aaattgaatc atttaacacc aaatgtcaaa gcaatcgtgg cagatgagat caaaatgtat
    14761 atgatcaatg aagaagcctc cgtggcagtg acattttctg gcgaagctgc cgatatgatg
    14821 tgggaaaatg aacatttaca ttatgtgatc ccaccagaag gatccaatct ttggtttgat
    14881 aatatcgtta tgccaaaaac cgtgaaaaat aaagaaggtg cctatgattt catcaacttt
    14941 atgctagaac cggaaaatgc ggcacaaaat gcagagtata ttggttattc tacaccaaat
    15001 gaagaagcaa aagcgttatt gccaaaagac atttcgtcgg acgaacaatt ctatccatct
    15061 gatgacacga tctcccatct tgaagtctac aaagatctag gatcgaaata cctaggcatc
    15121 tataatgacc tattcttaga attcaagatg tataggagat aatggcaaaa ctcagtaaga
    15181 atggggactt atcaacgttc taaagagcta aaaatcgtcc tttttctatc tgagttttcc
    15241 aatcttctac caaaagcgga tgaaacgccg ctttttttgt gttattttat tttttctacc
    15301 acgtttttct accagagaaa agtggacaaa accattgtaa aatagggccc gagacgggct
    15361 agtaaatcta agattgaaaa agttacagaa ggataaagta attggaaact atgcaaatat
    15421 acttaaccct tctgtaatct attaataaaa ctaatatatg aatccagttc aatatttgta
    15481 gctatgaaaa aacataaaaa tcatattaga taaaaactaa attggtagat aaattatttt
    15541 gtcagatatc tgaggcaaat attcgactat ttgtgtttca aagtaaaaat tggaatacaa
    15601 atatagaata gacatattgc agccgatagt ttattatgct tttagacaca ttaaactgga
    15661 ggaaaaaatg aaaaagagcg ctaaagatcg agtttttgat gagctgatac gactaacagg
    15721 gaatatgtct tctgtcactg ccgagaaaat ttcgaagaat ctaggtatct ctcgtcagaa
    15781 tgccagccat tatttgacga gattagtgga agataaaaag gtcgagaaat taccgggaaa
    15841 gcctgttttg tggaagcctt tagatgaata tgcggttgta gataatacgg agcaaataaa
    15901 cgaagccttt cattctgtag ttggtcatga cggaagtctg agagaggtca ttcaaaaatg
    15961 tatagccgct gtcaaatatc cgcctaacgg actcagtgta ttaataaatg gagccacggg
    16021 tgttggaaaa agttttttag ccacaaaaat ttttgaatac gctatccatg agcaaattat
    16081 tgaaaaagat gcaccatttg ctatcttaaa ctgtgccgat tatgcggaca atcccgaact
    16141 gctttcggca acactgtttg gatacaagaa gggggccttt acgggggctg aaaaggatac
    16201 cgatggttta ttggcaacgg ccaacaacgg ctatcttttt ctcgatgagg tccatcgcct
    16261 atcgaaagaa aatcaagaaa agctctttct atttattgat actggaaact atcgtccagt
    16321 cggtgaaaat gtaaattggc attctgctaa ggttcgtttt attttcgcca cgacggaaaa
    16381 aggtgaaaat tatttattag acacctttga tcggcgtatt caaattagtg ttatgttgcc
    16441 aacttttgaa gatcgcccca ttcgagaacg attagagctg attcaattat tttttcaaaa
    16501 tgaagcggat gttttacaaa aagatattat tgtttcaaga gaagccttgt ctttaatgtt
    16561 atctcatccc tttagtggga atattggtaa gctgaagaat atcattaaaa tcagttgtgc
    16621 ggatgtgtat agtcgaacac aagaagaacc attaacgatt gggaaaaatg agatcttgat
    16681 tcagttaaat atgctggaat ccgaagtaga aagcttgccg attgctagtt tattgataaa
    16741 gaaaaatcaa aagataaaaa cggatcgaat caaaccgttt gattatgatt cgattttgga
    16801 aaaaattact cagctgataa atgaaggtaa tttcaatgaa aatttttctt cgataaagaa
    16861 tgaagtacag catttggtgc atcacattga taaggattct tttttatcaa tgagtagaaa
    16921 attgatgatt cagatgttta gaaaggtctg ggtatccgtt ttaggaaaaa cgtacggatt
    16981 gacgacgagt ggacctgttg cagaaattat tagcagatcc tatgtcattt ccccaagcta
    17041 taagacagac ttaaccggga taaatcgtac attagctgca aatctacctc ggacgactta
    17101 cttatgtaat cagttcttgg agcatttacc accgctctca gaaaaggacg agaactttct
    17161 aagactgctt attgtcgtga ctttatctga ttatgtagat gaatccatcg agctgaaggg
    17221 cctgttgctt gctcacggag atagtacggc tagcagcatt caagcagtag tgaatcaatt
    17281 atgcggcaat tatgtttttg aagcgttgga tatgccagta gagagtaatg tgtcagagat
    17341 tgtcgaatcg acaaggaatt tcgtgaaacg gcaagtgaat acagatagtc tagtgctgat
    17401 cgtagatatg ggctctttaa atcaaattta cacacagatc aagaacgatt taaaagggga
    17461 gttacttctt ctgaataatt tgacgacttc cattgcactt gatattggct tgaagatgac
    17521 gatgaatgcg ccatttaaac agattgcgga ggatgcagca gacaagtatt caatcaatgt
    17581 tcaatacttt gaaggctttt ctcaaagtag caacattatc atttcctgta tgtcgggttt
    17641 ggggatttcg gacaaattga aagatgtttt tcgtcatcat ttatcggatg aatccatcga
    17701 agttttcacg aaggactaca gagaactacg tgaattaatc gaccaaaatg atgaaactta
    17761 ttttgacaag acaaaattgg tcatcacgac ttcggatctt ccacgttcct tttcaattcc
    17821 taatattaat gtttatgaca tcttagacgg tgatggagca gccaatttgg agcatatttt
    17881 gagtcctgaa atgaagccgg cagaatttaa ccgcttgatt caggagctag taaaattctt
    17941 ttcgttggaa ggaattgcgg accgtttgaa ctttctgaac ccaacgatcg tgatgagtga
    18001 agtggaaaca gtcatttcta aatatgaaaa ttactatcat ttcactttaa atggccgtgt
    18061 aaaactaaat ctctatatgc atacggcatt gatgatggaa cgtttattct tggctcggac
    18121 agaaaaatct gaagaaccga attatgattt gaaaccagat gaagatgaat tttattatgt
    18181 ttctagaagt atttttcaat caatggagat gaaatataat tttaaagtca atgcttatga
    18241 gctttcgctg ctctatgaat tattaggacc gtatattcaa aaataaagtg tctaaaaaag
    18301 tgtctaatct gtttttttta ggttagacac tttttttaat gtgaattaac gtgattaaac
    18361 caatgttttt aaaagttggc acaggaattg cttatataaa agtgaacaga aagaaaggaa
    18421 ttaaccatat gaagaaaatt ctaatagcga ctcacggtca cttagcttca ggctttctta
    18481 gcagtattca attactgacg gggaaggttc aagagattac ggtgattaat gcttatgtgg
    18541 atgagtgtga ttttgaacag gagttgaatg tatttgtaaa ccaggtgaac gagcaggatc
    18601 agatttttgt ttttaccgat ttatttggcg gaagtgtcaa tcagaagatt accaaaacct
    18661 ttttggagaa agagattgct gtaactattt tgtctggctt taatctgcca attctgctcg
    18721 aaatagtatt ggcaaccaag gaattaacaa aggaggatat tcaggaactg gtaaataaat
    18781 gccaacagga attaaagatg accattcttg atgaggtaca tgtagtaaat gaagaggaag
    18841 atttttttta ggaggaagaa aaatgattaa agcagtgcga attgatgaac gattaattca
    18901 tggacaagta gcgatgactt ggacaaagag tctaaattta accgggttag tagtagcaag
    18961 cgatgaggcg gctaccaacg atattcaaaa gatgacattg aagatggctg caccatcgaa
    19021 tgtcaaagtc attattaaaa cagttgatga cgcgattcat ttgttaaaag atccgcgggc
    19081 gaaggacatg cgcttgatgg ttttagtccc taatgtcaaa gatgcggtaa aagttgcgaa
    19141 agctttccca acggaaatcg aattaatgaa cgtggggaat gctgggaaaa tgacaccgcc
    19201 atcggaagat aagaaaacct tatcaaagga agtgatgtta acgacgtcag aaattcaaga
    19261 cttaaaagaa ttagttgaac tttatccaga taccgttttt cagcctacac cggcgatgga
    19321 aaaacgttca gctatctcta ttttgagtga tttttagaaa aaggaaggag aaaaattatg
    19381 tatgatgcat ttgtagtcgc tttggccgtt tttattggaa ttgctggaca tgaaatgttc
    19441 gggatgtcca tgctgagtcg tccgattgtt gttgctccgt tagcaggagc gttgatgggg
    19501 gatttgcaga cggggttgat tgtcggggcg tcactagaag ccatctttat gggtgtcgtc
    19561 aatatcggaa tctctagtac ggcggagcct tctttggctg ctgggctggc tacagcgttc
    19621 gcaatcaaaa tgggaggaaa cgtggatgcg attattccca ttgtctttcc actagcagta
    19681 ttaggattac agttaatgaa tatgattttt tcttttgtat gtggaccgat ggctgctaaa
    19741 tttttcaaat ttgcaaaatc tggtgatcaa aaaggaatca tcaatctgca cttctttatg
    19801 tggtttgtcc actatgcgct ttatgctctt attccattct ttgccgtttt atttggagca
    19861 gatgttgtca aaagcgtctt ggataatatt ccagaggtca tcatgaacgg attaacagtc
    19921 gctggaaatc tgctgccggc agttgggatg gcgatgctgt tacggatgct ttggagtaaa
    19981 aatattgctg tgtggttttt ctttggggcc gttgttatgg cgtatctcga gttaccattg
    20041 attgcaattg ccgcagttgg aacgattatt gcaattgtta tagcccaaag agatctacaa
    20101 ctaaaaaata tatctgttca tgcagttcaa agcgatgctg ctgtaaatgc aacgcttgac
    20161 caagaagagg aggatttctt tcaatgaaat tagctgatgg aatttctaaa gatgagaaaa
    20221 aattggttcg gaagatgttt tggcgctcga tgactatgta tgcttcggtg aatccggtga
    20281 cgatgggcgg cggtggtttc gcctattcta tgatgccctt cattaatcat ttctacaaaa
    20341 gtgaagaaga tcgagctgcg gcgctggaac gtcatacaca gtatttttcg acgaccattc
    20401 cttgcgcgtc ctttgtcatg gggattgcgg catcgatgga gaaggaaaat tctgaaaaag
    20461 ctggttttga cgtggaaagt atcaacgcga tcaaaacgag tttgatggga ccattggctg
    20521 gaattggcga ttcattgttc tggggagttt ggcgtgtcgt taccgcggga ctagcgatca
    20581 acttagccaa tgccggaaat attttggcac caattatctt tttagttatg ttcaatatac
    20641 ctaattactt gatgcgatac tatggcggcg ttttaggcta ttcattaggc tctaaatata
    20701 ttgaaaagct gtatgcgaac gggttaattg aaattctgac aaaagctgct agtatcttgg
    20761 gtctgatcat ggtaggtgca atgacgatca gtatggtcaa attcaatacc gttttgagct
    20821 tttcaatgaa gggcgaagaa ctaatgaatg tccaagaaat cctcgatagt atttttaaag
    20881 gaattgtacc gttattggta acattgagct gtttcaaatt actagataaa aaagtcggcg
    20941 tcatcaaaat tatttttgga atggtcgtct tgggaatttt attgtcatta cttggaatcg
    21001 catagtagga agctgcagct actaggctgc agttttttta gcaaggaggg aaaagatgac
    21061 tattaatgaa gtaatttatt cacccaaaca cccatttttt aatgctgagt taaacgacgg
    21121 aaatgtgact acttcaaacg aatacagcta ctatcggaat attcctgatg taaagggatt
    21181 gaccttcgat gtaagtaatc aaaagacatt agcaaacctt gatgtttgcg ggacgattca
    21241 aacgttgacc ttttatcgag aaaatttact aactgaggaa aaaccaggtg tttgggtcaa
    21301 taaacagctc tctcaaacca attcgttatc cttaaaggtt gaagtaaacg ggattgtcta
    21361 ccatttgtca gaatcggatc atcgtattga agtcgatgtg attcaagact gtttgcctag
    21421 atatattcat cattattcga cgtttaaggt cattgtagta tcctttgctc ccatactggc
    21481 taataagcgt ttaagtatgc ttgttcaaca ggtttacatt gtgaatgaaa cacacgaaag
    21541 ccttcaagtt tcagtgaaag atgtgccttc gtatcaagag aagtattcgg atcagcaaaa
    21601 tgtgttgatt tctcaaaaag gaaataagga cacgatcgca caacaggaag tggcaagctt
    21661 cgccattgct tttgtggacc caaatgcttt tgaagaagta atgacttttc aggaatctga
    21721 tatcgaaaaa tggctgaagg aaacattgaa ctattttgct cagctatttg ggcagctgtc
    21781 aatgccaaac gatagagttg tccatctcta tcgtcgtgca ctttatcaaa gcttttctag
    21841 tttcggtatg aatcgacagg gacaggtcgt cggcagcaat tggggcagct atcctgctac
    21901 taatcgaatt tggaataagg atatgtatta cgcatcattg ccattcacca tgtttgaacc
    21961 ggagttatgt caaaaaacgg ttctatggtt tgatcaatat ggcgttaagt ttcctggttc
    22021 aaaatttaca ggaggtatca atcattctct gagtaattcg ttatccagtg cgttactggc
    22081 tagtctttat tttgaacata ctggggatac aaaattcttt gagcggtgtc cgcaggttct
    22141 actgaatgct tcaagaatta ttgaggacat attagctcaa cgtcggtcag gggaaccaat
    22201 gctttttcca tctgtttggt tatcagacgc ctttgcactt ggaaagtatc ataccggcag
    22261 caatctttgt ctttggaagg cctgtattgg acttgcagaa atttttgaag ccctagaaca
    22321 actgagtctt agcaagcgat acaaaaacat tgcctgtaaa ctaaaggaat caatttgtaa
    22381 acaaatgact atcgatggtc cttttggtga gcaatttttg gagggaatcg gtgatgagga
    22441 gaaaacaacc tattcagtga agcactatca aaaaccgatt cttgagcaag ggttaatctt
    22501 tttaagtgat gtgattgtgg atggaaaaat taatttattg atgcatgatg gagaagaatc
    22561 agacactacg ttgatgcctt tttatcaatt tttagataat aaagatcatt tatatcagaa
    22621 caccatggcc ttttctgcta gttctttcaa tccaacctat agcgaggaaa tcaaaggcat
    22681 cacttgggga ttagaatccg gcgccacttt tcctggcttt atcactgttt taatgagcga
    22741 gctgcacaac cctcaagctt tcgtaacccg attagaagaa ctaatttgtt tggcagattt
    22801 agacggttcg tggtggtggt ggccgtatcg cttagaggct caacaaggag aagtcgtgcg
    22861 agactttggc tgcggcaaat gcggctgggc atcgggtctt tttgtcagcc taatgatcag
    22921 tcaatatttc ggattgaaat tcaaaaaagg agtattgcaa attgatcctg ttgataattt
    22981 aacgttttct tggaagaatt tgaagttcgg tcaagccttc atttcgattg aatgtacgca
    23041 atccgagttg agtatttcaa atttgtcaga aaagccattg aaaataagtg atacgaaaaa
    23101 aattcttcac gtagaaaagg gaaaaacgat tcacattcaa aggagaaaaa gatgagtata
    23161 ctaaaaaata ttacccaatt taaaaaaatc cagagtcgag caatcactcc cgaaaatcca
    23221 acaggcgaga aaggtaaagc tgcgatgttg ccaagcgagc taggaccaag ccgcaaaggt
    23281 cgtggcgcaa ttccgctgac ttctggtgaa gaaactgtga ttgcggatat tactggaacg
    23341 ggaataattc gtcatatgtg gatgacgatt cgtgatcgaa cagaatttgg cagttttgtt
    23401 ttacgtgatt taatcttaag aatttattgg gatgggtcgg atagccctgc tgttgaaagt
    23461 cctttagggg atttcttttg taatgggttt ggagagcgtt gcgaggtgaa ctcgttgccg
    23521 atagtggtta atcctacagg gggctttaat tcctatttcg aaatgccttt ccaaacatcc
    23581 gctaaaatta cgatcgagaa tttacatccg aaggatatgt cctcattttt ttatacgata
    23641 aattacgctt tggtggacga attggaagaa aatagcctat attttcacgc aaattggcat
    23701 cgggaacgca ttaccaaatt acaggaggat tataagttaa ttgatcgatt aaagggtcaa
    23761 ggctattacg tgggtactta ccttgcgctt acctgcctag aacgttattg gtggggcgaa
    23821 ggcgagttta aattctacat tgatggggat agtgagtatc cgaccgtaac ctcaacaggt
    23881 tcagaagatt attttggcgg ggcgtgggcc tttcatgagt acgatgagtt cggacgtcca
    23941 catgcgaaaa actattcaac agccttttta gggtatcctt accaatcaaa tagagatgcc
    24001 acaagagatc gttttgaaac gggaaaattg aatgccgttc atgcctttgg tgacgacgca
    24061 atgccacgac atggtcttta tcgttggcat cttcaagacc ccatctgttt taccgaagat
    24121 ttatcggtca ctttacaaca aatcggcaac gatgatgttc gcttatttga gcggcaagac
    24181 gatgttgcta gtgtcgctta ctggtattca acagaagctt caggaaatac taaacgtcat
    24241 ttcactgatc gagaacgctt gccgcgttaa tgaagtgttt ttcacatatt cgaagggagg
    24301 aacctatgat gatatatggg gcaattgaag ctggagggac taaatttgtc tgtgcagtcg
    24361 gtactgagga attaacgatt ttggaaagag tcagttttcc cacaacaact ccagttgaaa
    24421 caatgaagca ggtcattgat ttttttaaac cttttaagga acaattggca ggcatcgggg
    24481 tcggctgctt tggtccaatt gatattcaat gcaaatcaac gagtttcggt catatcacat
    24541 caacaccaaa acttgcttgg caaaatttca attttgtagg aacgctaaag caatatttca
    24601 atattccgat tgaatggaca acagatgtca atgcggcttg ctatggtgaa tatgttgctg
    24661 gttctggcaa agggttagca ggtgttgcat acttcactgt gggaacaggt atcggaggtg
    24721 gtgccttgtt caatgggact ttcgtggaag ggttcagtca tccggaaatg gggcatatgt
    24781 tgatcaagcg acatccaaag gacaattttt caggaaactg tcccttccac caagattgtt
    24841 tagaggggat ggcagcagga ccagcgattg aaaaacggtt gggtgtaaaa ggccagaatt
    24901 tattggctga cgattcgttt tggcagattg aagcctttta ccttgcccaa tgtgcataca
    24961 atacaacact gatgttttcg ccagatagaa ttatttttgg cggtggtgta atgaaacaag
    25021 aacacatgaa gaaaaaagtt caggataaat ttgttgagct tataaatgga tatgttgaaa
    25081 taccaccaat tgatagctac attattacgc ccgaattagg tgataatgcc ggaataatcg
    25141 gcggattagc attggccaga aaagcggtaa gaaataagca accataatta atgaatgttt
    25201 cattgatact catgtcaaaa gtatttaacc gagtaatcaa atgatcaaat gaagttggat
    25261 aaagcgaaaa tgaattcaaa taaaaaggct ctaagtcaaa tcgtgttgtt ttcattaaat
    25321 cactgaaagg aaatgcttct gcgtttcctt ttttcttttt gttgtgcgct gtataccttg
    25381 aggatattca ctggtggcgt acacttaaat agtaatatat tgtgttactt tatgtctgtg
    25441 gtaatagtta tagttgctta ccaatgtatg ttcatttttc gaaatgttga cttcataatc
    25501 cttggcttaa tgtttgaatt tttttggtga tagatttttt cagccgtata aaataaatcg
    25561 taattctatt atagagagtt gtgattataa aaagatagca agaaaaaaaa agaaagtttc
    25621 tatacatttt aaatatttta ttcctatttc tattgctaaa aaagtagaaa ttctatattt
    25681 acctttaact ctcgcttctc ttgcaaatgt agttagcatt ttaatcgata ataaactcag
    25741 gtagttatat taccttgaaa tgattgataa ttttgtcact tctatttttt ttgcaataaa
    25801 ttttttattt tagtgaaaaa taagaaacta tttctttgaa ggcgataagt tagaaaaata
    25861 ttattgtagt cttttc
//
LOCUS       DARDHU010000028        25194 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000028 DARDHU010000000
VERSION     DARDHU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.028"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..746
                     /locus_tag="SDD03_001228"
     CDS             99..746
                     /locus_tag="SDD03_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG4303681.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="SDD03_001229"
     CDS             1139..3784
                     /locus_tag="SDD03_001229"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG4303682.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="SDD03_001230"
     CDS             4094..5407
                     /locus_tag="SDD03_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG4303683.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="SDD03_001231"
     CDS             5397..6050
                     /locus_tag="SDD03_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4303684.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="SDD03_001232"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="SDD03_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="SDD03_001233"
     CDS             6787..7872
                     /locus_tag="SDD03_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4303685.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="SDD03_001234"
     CDS             7869..9761
                     /locus_tag="SDD03_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4303686.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="SDD03_001235"
     CDS             9768..10145
                     /locus_tag="SDD03_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4303687.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="SDD03_001236"
     CDS             10299..11081
                     /locus_tag="SDD03_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG4303688.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="SDD03_001237"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="SDD03_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG4303689.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="SDD03_001238"
     CDS             complement(11996..12055)
                     /locus_tag="SDD03_001238"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG4303690.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="SDD03_001239"
     CDS             12447..13109
                     /locus_tag="SDD03_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4303691.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="SDD03_001240"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="SDD03_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13637..13837
                     /locus_tag="SDD03_001241"
     CDS             13637..13837
                     /locus_tag="SDD03_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4303692.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="SDD03_001242"
     CDS             complement(14091..15770)
                     /locus_tag="SDD03_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG4303693.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="SDD03_001243"
     CDS             complement(15772..15984)
                     /locus_tag="SDD03_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG4303694.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="SDD03_001244"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="SDD03_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG4303695.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="SDD03_001245"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="SDD03_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG4303696.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="SDD03_001246"
     CDS             19959..20156
                     /locus_tag="SDD03_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303697.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="SDD03_001247"
     CDS             20309..20794
                     /locus_tag="SDD03_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG4303698.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="SDD03_001248"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="SDD03_001248"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG4303699.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="SDD03_001249"
     CDS             21671..22198
                     /locus_tag="SDD03_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303700.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="SDD03_001250"
     CDS             22289..23026
                     /locus_tag="SDD03_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG4303701.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="SDD03_001251"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="SDD03_001251"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG4303702.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="SDD03_001252"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="SDD03_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG4303703.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8390 a         4103 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DARDHU010000029        24980 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000029 DARDHU010000000
VERSION     DARDHU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.029"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(451..1233)
                     /locus_tag="SDD03_001253"
     CDS             complement(451..1233)
                     /locus_tag="SDD03_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303704.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="SDD03_001254"
     CDS             complement(1298..2134)
                     /locus_tag="SDD03_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4303705.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="SDD03_001255"
     CDS             complement(2152..3561)
                     /locus_tag="SDD03_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4303706.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="SDD03_001256"
     CDS             complement(3575..5398)
                     /locus_tag="SDD03_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4303707.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5678..5905)
                     /locus_tag="SDD03_001257"
     CDS             complement(5678..5905)
                     /locus_tag="SDD03_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303708.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6241..6567)
                     /locus_tag="SDD03_001258"
     CDS             complement(6241..6567)
                     /locus_tag="SDD03_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303709.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="SDD03_001259"
     CDS             complement(6613..6975)
                     /locus_tag="SDD03_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG4303710.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="SDD03_001260"
     CDS             complement(7012..7227)
                     /locus_tag="SDD03_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4303711.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="SDD03_001261"
     CDS             7354..7689
                     /locus_tag="SDD03_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4303712.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="SDD03_001262"
     CDS             complement(7765..9762)
                     /locus_tag="SDD03_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303713.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="SDD03_001263"
     CDS             complement(9860..10135)
                     /locus_tag="SDD03_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303714.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="SDD03_001264"
     CDS             10367..11101
                     /locus_tag="SDD03_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4303715.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="SDD03_001265"
     CDS             11140..12282
                     /locus_tag="SDD03_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4303716.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="SDD03_001266"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="SDD03_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG4303717.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /locus_tag="SDD03_001267"
     CDS             complement(13773..14243)
                     /locus_tag="SDD03_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG4303718.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="SDD03_001268"
     CDS             complement(14230..15465)
                     /locus_tag="SDD03_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4303719.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="SDD03_001269"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="SDD03_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG4303720.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="SDD03_001270"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="SDD03_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG4303721.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="SDD03_001271"
     CDS             complement(17731..18579)
                     /locus_tag="SDD03_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG4303722.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="SDD03_001272"
     CDS             complement(18603..19289)
                     /locus_tag="SDD03_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303723.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="SDD03_001273"
     CDS             complement(19282..20316)
                     /locus_tag="SDD03_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4303724.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20379..20635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20711..21049)
                     /locus_tag="SDD03_001274"
     CDS             complement(20711..21049)
                     /locus_tag="SDD03_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG4303725.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="SDD03_001275"
     CDS             complement(21051..21407)
                     /locus_tag="SDD03_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG4303726.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="SDD03_001276"
     CDS             complement(21413..22600)
                     /locus_tag="SDD03_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG4303727.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="SDD03_001277"
     CDS             complement(22791..23636)
                     /locus_tag="SDD03_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4303728.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="SDD03_001278"
     CDS             23870..24835
                     /locus_tag="SDD03_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG4303729.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7309 a         5247 c         4241 g         8183 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa
//
LOCUS       DARDHU010000030        24575 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000030 DARDHU010000000
VERSION     DARDHU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.030"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SDD03_001279"
     CDS             complement(152..550)
                     /locus_tag="SDD03_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG4303730.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SDD03_001280"
     CDS             complement(547..1320)
                     /locus_tag="SDD03_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG4303731.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SDD03_001281"
     CDS             complement(1317..2000)
                     /locus_tag="SDD03_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG4303732.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SDD03_001282"
     CDS             complement(2167..3054)
                     /locus_tag="SDD03_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4303733.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SDD03_001283"
     CDS             complement(3051..3920)
                     /locus_tag="SDD03_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4303734.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SDD03_001284"
     CDS             complement(4057..5343)
                     /locus_tag="SDD03_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4303735.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SDD03_001285"
     CDS             5616..6194
                     /locus_tag="SDD03_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4303736.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SDD03_001286"
     CDS             6194..7123
                     /locus_tag="SDD03_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4303737.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SDD03_001287"
     CDS             7120..8856
                     /locus_tag="SDD03_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4303738.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SDD03_001288"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SDD03_001288"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG4303739.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="SDD03_001289"
     CDS             complement(9752..10261)
                     /locus_tag="SDD03_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG4303740.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SDD03_001290"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SDD03_001290"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG4303741.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="SDD03_001291"
     CDS             complement(11562..12653)
                     /locus_tag="SDD03_001291"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG4303742.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SDD03_001292"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SDD03_001292"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG4303743.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SDD03_001293"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SDD03_001293"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG4303744.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SDD03_001294"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SDD03_001294"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG4303745.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="SDD03_001295"
     CDS             complement(16616..17590)
                     /locus_tag="SDD03_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG4303746.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="SDD03_001296"
     CDS             complement(17571..19454)
                     /locus_tag="SDD03_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG4303747.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="SDD03_001297"
     CDS             complement(19472..21697)
                     /locus_tag="SDD03_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG4303748.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="SDD03_001298"
     CDS             21812..22846
                     /locus_tag="SDD03_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4303749.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="SDD03_001299"
     CDS             complement(22913..23926)
                     /locus_tag="SDD03_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4303750.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="SDD03_001300"
     CDS             complement(24004..24192)
                     /locus_tag="SDD03_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG4303751.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DARDHU010000031        24313 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000031 DARDHU010000000
VERSION     DARDHU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.031"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            136..858
                     /locus_tag="SDD03_001301"
     CDS             136..858
                     /locus_tag="SDD03_001301"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG4303752.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            861..1418
                     /gene="frr"
                     /locus_tag="SDD03_001302"
     CDS             861..1418
                     /gene="frr"
                     /locus_tag="SDD03_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG4303753.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            1613..2425
                     /locus_tag="SDD03_001303"
     CDS             1613..2425
                     /locus_tag="SDD03_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG4303754.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            2422..3222
                     /locus_tag="SDD03_001304"
     CDS             2422..3222
                     /locus_tag="SDD03_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG4303755.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            3383..4651
                     /gene="rseP"
                     /locus_tag="SDD03_001305"
     CDS             3383..4651
                     /gene="rseP"
                     /locus_tag="SDD03_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG4303756.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            4719..6428
                     /locus_tag="SDD03_001306"
     CDS             4719..6428
                     /locus_tag="SDD03_001306"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG4303757.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            6640..10998
                     /locus_tag="SDD03_001307"
     CDS             6640..10998
                     /locus_tag="SDD03_001307"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG4303758.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            11143..11616
                     /gene="rimP"
                     /locus_tag="SDD03_001308"
     CDS             11143..11616
                     /gene="rimP"
                     /locus_tag="SDD03_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG4303759.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            11639..12814
                     /gene="nusA"
                     /locus_tag="SDD03_001309"
     CDS             11639..12814
                     /gene="nusA"
                     /locus_tag="SDD03_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG4303760.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            12836..13129
                     /locus_tag="SDD03_001310"
     CDS             12836..13129
                     /locus_tag="SDD03_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG4303761.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            13126..13437
                     /locus_tag="SDD03_001311"
     CDS             13126..13437
                     /locus_tag="SDD03_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG4303762.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            13450..15756
                     /gene="infB"
                     /locus_tag="SDD03_001312"
     CDS             13450..15756
                     /gene="infB"
                     /locus_tag="SDD03_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG4303763.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            15783..16130
                     /gene="rbfA"
                     /locus_tag="SDD03_001313"
     CDS             15783..16130
                     /gene="rbfA"
                     /locus_tag="SDD03_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG4303764.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(16287..16460)
                     /locus_tag="SDD03_001314"
     CDS             complement(16287..16460)
                     /locus_tag="SDD03_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4303765.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            16908..19270
                     /locus_tag="SDD03_001315"
                     /pseudo
     CDS             16908..19270
                     /locus_tag="SDD03_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            19638..20561
                     /gene="truB"
                     /locus_tag="SDD03_001316"
     CDS             19638..20561
                     /gene="truB"
                     /locus_tag="SDD03_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEG4303766.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            20565..21506
                     /gene="ribF"
                     /locus_tag="SDD03_001317"
     CDS             20565..21506
                     /gene="ribF"
                     /locus_tag="SDD03_001317"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG4303767.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            21858..22643
                     /locus_tag="SDD03_001318"
     CDS             21858..22643
                     /locus_tag="SDD03_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4303768.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            22656..23507
                     /locus_tag="SDD03_001319"
     CDS             22656..23507
                     /locus_tag="SDD03_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG4303769.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(23631..24083)
                     /locus_tag="SDD03_001320"
     CDS             complement(23631..24083)
                     /locus_tag="SDD03_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303770.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         8290 a         4244 c         5307 g         6472 t
ORIGIN      
        1 ttctgttgaa taacagacgg gaacgcacgg tttgtgcgct cccttttttt aagcaaaaca
       61 agaaaaacca gtcttagagt gtcgaaatca tctaagtatg gtaaaatggc aatagaaaat
      121 attggaggaa tgaacatggt cacaccgaaa tatcagcgtg tagtcttaaa attgagtgga
      181 gaagctttag ctggggacgc aggatttgga atcaaaccac cagtaatcaa agaaatcatc
      241 caagaaatca aagaagttca cgagctgggc gttgaaatgg caatcgttgt gggcggcgga
      301 aatatctggc gcggacaaat cggagcacaa atgggcatgg aacgtgctca agctgattac
      361 atgggtatgt tagctacagt tatgaatgcc ttagctttac aggatacgtt ggaaaacgtg
      421 ggtgtaccga cacgcgtgca aacttcgatt gaaatgcgtc aaatcgcaga gccatatatt
      481 cgccgtaaag cagaacgtca tttagaaaaa ggacggatcg ttatctttgc aggcggtacc
      541 ggcaacccgt acttctcgac agacacaaca gcagcattac gcgcggcaga aatcggcgca
      601 gatgttatcc tgatggctaa aaacaatgta gacggtgttt attctgctga tccgaaagta
      661 gatgcaaatg cagtaaaatt tgaagaactg actcatctgg aagttattgc caaaggatta
      721 caagtcatgg attcaactgc aagttcacta agcatggata atgatattcc actgcttgtc
      781 ttcaatttaa atgaacacgg aaatattcgc cgtgcaatct taggcgaaaa tatcggaaca
      841 actgtaaggg ggaaataaaa atggcagatg caattatgac agaagcaaaa gaaaaaatga
      901 aaaaagcagc acaaaatctt caacgtgaac ttgggcaaat ccgcgcaggc cgtgcgaacg
      961 ctagcctttt ggaccgtatt actgtgaact actacggtgc gccaacacca ttgaaccaaa
     1021 tggcatcaat ccaaattcca gaagcacgtg ttttaatgat cacacctttt gacaagagca
     1081 ttcttcaaga tgttgaaaaa gctattatgg caagtgatat cggaatcagt ccaaccaatg
     1141 atggaaatgt cattcgctta gttatcccgc aactgacaga agaacgcaga aaagaattag
     1201 ctaaagatgt taaaaaagaa gcagaaaatg caaaaatcgc gattcgtaac atccgtcgtg
     1261 atgcgatcga tgaatataaa aaacaacaaa aaaatggtga tattaccgaa gatgatttgc
     1321 gcggattaga aaaagacgta caaacattga cagataacag catcaaagaa gtagataaga
     1381 tcgctgctga taaagaaaaa gaattattgg aagtttaata aaatcataaa aacagagggg
     1441 cggatcattc cgcttcctct gtttttattt atatttcttt tatttaaaaa ttaaaaaaag
     1501 aaaaaattat tcagttttta aataaaaaaa caatgatttc ataggaaaaa cagttagata
     1561 ttgctataat acttaagata gtgggaatct tttaagtata ggaggaaaaa gaatgttacg
     1621 tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat ttaataccga
     1681 tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt gggcgcaaaa
     1741 ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa agaaagtcac
     1801 aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt cgactgaaaa
     1861 ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg acttctttga
     1921 tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg gttatgaaaa
     1981 cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa caaaaaacaa
     2041 tactggaatg gtattgaatt ttgctttgaa ttatggaagc agagcagaga tcgtgactgc
     2101 tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag aaatcgatga
     2161 tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac aagatcctga
     2221 gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat ggcagatcgc
     2281 atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg cgcatcttga
     2341 agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa aaaaaacaga
     2401 agatgagaaa ggggatcagt catgaaacaa cgtgtggtca cagcagccgt ggcgctagtc
     2461 ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc agcaatctta
     2521 gcagtaatag gagtttacga gctgtttcgg atgaaaggat tgacactctt aagttttgaa
     2581 ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag atggtttttc
     2641 tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat gattctttta
     2701 ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt tcctgtgctt
     2761 gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac agaaagcttg
     2821 gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc atatatggtt
     2881 ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa aacgattgaa
     2941 ggtgcgctcg gtggtattgg tagtgctgtt gtagtcgcat ttctttattt attgctatac
     3001 ccagcaaaag aactatttaa tcatggtatg tttgtcatgc ttctgatgac gattattttt
     3061 tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca ttatgatgtg
     3121 aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt cgacagtttg
     3181 ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata cctgcgtttc
     3241 tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata ctattggtgt
     3301 ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa aacacaaaaa
     3361 acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg tttttggtat
     3421 attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag gaatcctcgt
     3481 tcgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa aagacgggac
     3541 aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag gcaatgggga
     3601 tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt ctgacggcat
     3661 cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc cgatggaatt
     3721 gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg gagacgaaac
     3781 agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg acggaacaga
     3841 aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc agcgtatgct
     3901 gaccaacttt gctggaccca tgaataactt tattttggca atcctgttat tcatcattct
     3961 ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag agatcatgcc
     4021 taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca gtgtggacgg
     4081 aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc cggataaaac
     4141 attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga caccgaaatc
     4201 tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa tgaatacagg
     4261 ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat tggaaatttt
     4321 caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg gacctgtcat
     4381 gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta ttagtttgat
     4441 ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg ccttagatgg
     4501 cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa gtcaagaaaa
     4561 agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg tgctagttac
     4621 atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg tataataaca
     4681 aacgataact attttgagga aataaaggag tatatagaat gagacaatca aaaatgttga
     4741 ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa atcttgctaa
     4801 gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca ttagcaaatc
     4861 gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc ggagcggtag
     4921 aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg cgttacgaaa
     4981 catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg atccttggac
     5041 caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca tataagaaat
     5101 tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga cctcgcttcg
     5161 gtttgcttcg cggaagagag tttatcatga aagatgcgta ttctttccat gcaacggaag
     5221 aaagtttgga cgagacatat aaagattacg aaaaagccta caccgaagta ttcaagcgtt
     5281 gtggcttaga atttcgctcg attatcggag acggtggcgc aatgggcggg aaagactcaa
     5341 aagaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg actgaaagtg
     5401 attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa tcgcatgaaa
     5461 cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac gaagtagcag
     5521 atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt gcggatgaac
     5581 aacctgtcct tgttttggta cgcggagacc atgaagtcaa tgacgtaaaa ctgaaaaact
     5641 ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa tatctaggtg
     5701 ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta tatgcagacc
     5761 gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga ttccactata
     5821 aaaatgtgaa tccagaacgc gatttcactg tactttctta tgaagatctt cgttttgtac
     5881 aagaaggaga tccttcacca gacaacaatg gtgttcttgc ctttactcga ggaatcgaaa
     5941 tcggacatat cttcaaaatc ggcactcgtt acagtgaatc tatgggagca actgtactag
     6001 acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt gtaagtcgtt
     6061 tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaactgg ccaaaaggaa
     6121 ttgcgccatt tgatttgcat atcgtccaaa tgaatctaaa agacgaatac caaacaaacc
     6181 tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg gttgatgaca
     6241 gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt ccaatcagaa
     6301 tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag aaaactggcg
     6361 aaatggttga agtacgtaaa gaagaattgg tgaatacttt accaatcctc ttgaaccaag
     6421 gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg tttttgacaa
     6481 tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca attttttaat
     6541 gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg ataagatgcg
     6601 atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa acgagattta
     6661 tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca tccactgctt
     6721 actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg ggaatttacg
     6781 ctgcattttt ccaaaatttt gcctattatg ctatatcggt cattgactca gcatctgact
     6841 atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga ccagacattc
     6901 gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca atgcgataca
     6961 ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg taaagtcatc
     7021 ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt gccattagta
     7081 gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga agtggatgag
     7141 cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca ggcagaagcc
     7201 tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa ggaaagaaaa
     7261 gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa tattccagcc
     7321 gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt cacacttgag
     7381 gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat cttgactttg
     7441 aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg cgaaaaagat
     7501 gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg aagtattcaa
     7561 gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga agtcaaacat
     7621 acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca tgttcatagc
     7681 aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca agcagggaaa
     7741 tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt tccggaagca
     7801 catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc gaatgtcgta
     7861 gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga agccacatat
     7921 gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat catcgagcta
     7981 gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt tatcgatcca
     8041 ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg aatggttcga
     8101 ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa agatacaatt
     8161 ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta cgcaaggtat
     8221 ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag atatctgtat
     8281 cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt cagcttggaa
     8341 cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg gatctttgtg
     8401 aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga gcatatcgga
     8461 gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc aaaaaatcag
     8521 gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata cttcgaacgc
     8581 gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt gatcggctca
     8641 gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga agaggcaaga
     8701 aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta tgcgcctttg
     8761 atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca aaatttggta
     8821 gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca ttatctgaac
     8881 gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc gaatccgttg
     8941 aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct aactgcattt
     9001 cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa taaaatcgca
     9061 gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa gattccaggt
     9121 tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta cggagatccg
     9181 ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa tggtaatggc
     9241 ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga agatggctat
     9301 ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac aggaatcact
     9361 gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc cgagtttttt
     9421 gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc aaaatgtggt
     9481 gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg attccatgga
     9541 gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga agcccataac
     9601 tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat tggtacagtc
     9661 gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa tctgcagttt
     9721 cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg gacaaccggc
     9781 cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga ttttacgccg
     9841 atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt tgattttcac
     9901 tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc aactgttatt
     9961 cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga tgacccggaa
    10021 gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca aatttattcc
    10081 aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg gatgctagaa
    10141 gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc tcatggtaca
    10201 gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac actggcggaa
    10261 gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt agatagcggg
    10321 atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat ccctgatgaa
    10381 ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg gtatatcgat
    10441 tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta cgtattgatg
    10501 gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg tgcatacttt
    10561 tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga tgctgtgaaa
    10621 caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga aaagaatcaa
    10681 ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt tggaatgatt
    10741 gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt gatcgcacca
    10801 tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga agcaagaaaa
    10861 gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc aaaaacattg
    10921 atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa tcaattatcg
    10981 ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa gcagagtatg
    11041 gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg ctcactcttt
    11101 ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag cgtcgttgaa
    11161 acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact tgtcgaagtc
    11221 gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa agaaggcggt
    11281 atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact tgatgcagca
    11341 gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg agcagagcgg
    11401 ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca tgtctcctta
    11461 taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt tgatgctgaa
    11521 cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt tgaccgaaaa
    11581 aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat cagaaaaaat
    11641 gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga tctcaaaaga
    11701 aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc actatggcca
    11761 agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg tctatgcggt
    11821 aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga aagatgcact
    11881 tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa caccaaaaga
    11941 ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg tacgtgaagc
    12001 agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca tgcaaggaat
    12061 cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg aggcagttct
    12121 ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa tcaaagttta
    12181 tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc gcagccatcc
    12241 agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg gaatcgttga
    12301 aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc gctcaaatga
    12361 tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag ttcaagcaat
    12421 cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag atccagctgt
    12481 attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg atgagacaaa
    12541 tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa tcggtaaacg
    12601 cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata tcaaatcaga
    12661 atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag aagaactgca
    12721 tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa ttgcattcaa
    12781 caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca aagagatgaa
    12841 acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac ctaaaaaaga
    12901 gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa ctggaaaaat
    12961 gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag cttgggataa
    13021 aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc aagaacttct
    13081 tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa tacagttaat
    13141 cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact aatcacgggt
    13201 gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt tgtggcaacc
    13261 gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta tgaagttcct
    13321 tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc tcgcaaggta
    13381 ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga aggttaggaa
    13441 ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa aaaatctagt
    13501 aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca tatgggtgcg
    13561 attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag cacgaaacaa
    13621 caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga tcaacgtaaa
    13681 aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca agatcgcggt
    13741 caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa taatacggga
    13801 aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa ccgtactaac
    13861 aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa ccgtaatcgt
    13921 tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc tgtaccacca
    13981 cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa cgtagcagat
    14041 attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt tatgcttggt
    14101 gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt agcgacggac
    14161 tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga taaattcttc
    14221 gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt aacgattatg
    14281 ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc acgtgtaaca
    14341 agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga tattgatggt
    14401 aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat gcgtgcacgt
    14461 ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg cgtcatgcca
    14521 caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat cgtcgctgta
    14581 aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt aagtgagtat
    14641 gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc agcgaaattc
    14701 ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt agaagattta
    14761 aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct tgataaaggt
    14821 aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg tgacccaatc
    14881 gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg ccgtcgcgat
    14941 aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt acctcaagca
    15001 ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg tgaagaacgc
    15061 gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt ggataactta
    15121 tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa agcagatgta
    15181 caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga aggtgtccgt
    15241 gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac cttagcagca
    15301 gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc aaaagtgcaa
    15361 gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc catcgaagaa
    15421 atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat caccggtcaa
    15481 atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg tgcctatgta
    15541 acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg aatcgtaatc
    15601 tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga agtcaaaatg
    15661 ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga tgacgtaatt
    15721 gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg aggccaatca
    15781 ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa gtcaatgata
    15841 ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca gatgtgaaag
    15901 ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac ttagcatcag
    15961 ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt cgagagttag
    16021 ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat gaatcagttg
    16081 tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa tcaaaaactc
    16141 ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg ttttcattaa gacgtattac
    16201 ctaagagttc catgacttta gtcttgcggc gtgtcagcta accaaaaaag agaaaaagaa
    16261 aagtaaaaaa ttcgactttc ttttttctag tagtatataa atttttgcat atagataaag
    16321 atcaagatac cgataacgga agctagattg taatctattt tagtgacagt caggtatagc
    16381 caagtaacta gaagatcagt ggaacctgca ccataaaagt ttgagtttgt cggtccgttg
    16441 acagttaaaa gaaaaatcac gttgaagtta ttgatatttc cgattacgcc gcttcgattt
    16501 ggtcacgatc caaattttgg ataatccctg tagaaattag catagtagct gggataccaa
    16561 ttcacatatt gacaaagata acagagactt ttacataaaa cggatcaatc aggaaaggaa
    16621 ttgctcgatc cagccccagt tttccagcaa tgtgttaact ggcccagcgt cattcagcaa
    16681 attatgcatg atgaggagcg aaatgaactg cggaacagcc attgtgataa cgacaagttt
    16741 tttggatcaa tgtaaaaaac gtcagcgaaa ggaaaaattt cgtggttttt tatacctgtt
    16801 catttggcta gatataaaat ccaaacaaat gaacagaagt gcttattagg tttcaattgg
    16861 gagtatctgc gctagaataa gagtaataat gaaaaggagt gttatgtatg gattattcta
    16921 gtaaagaata ctttgataaa atgacggctt ggtggcgtgc tgctaattat ctttctgttg
    16981 ggcaacttta tcttaaagat aaccccttac tgagacgtac actgaaaccc gaagatgtca
    17041 aaaaacatcc aatcggtcat tggggaacga ttcctggcca aaattttatt tatgtccatt
    17101 tgaaccgagt catcaataaa tatgatttga acatgtttta tattgaaggg ccaggacacg
    17161 gcggacaagt catggtatcg aatgcttatt tagatggaag ttacacagag atctatccag
    17221 aagtaacgga agatgagaca ggaatgcaaa aattattcaa acgattttca tttcctggag
    17281 gcattgcttc tcatgcagca ccagaaacac caggttctat ccatgaaggc ggagagcttg
    17341 gatactctct ttcacatgca gtcggagctg tattagacaa tcctgaagta atctctgctg
    17401 tcgtgattgg tgatggtgaa gcagaaaccg gtcctttagc tggaagctgg ttctcaaatg
    17461 tctttatcaa tccagtgata gatggagctg ttttaccgat ccttcattta aatggtgcaa
    17521 aaatagccaa cccaacgatc ttggcacgta aatcagatgg agaattagca aattacttta
    17581 acggacttgg ctgggaacct ttctttattg aaggaaatga ttcagaaaaa ttaaatcctg
    17641 ttatggctga aaaaatggat caagcaatcg agaaaattaa atccatccaa aaagaagctc
    17701 gcctgaagac agcaacagat gtcgtgatgc caaaatggcc agtattgatc gtacgcacac
    17761 caaaaggatg gaccggtcca aaagaatggg acggggaacc aattgaagga accttccgag
    17821 cacaccaagt accgattcct gtagatcaag aacatatgga tcatgcagat gctttactcc
    17881 gttggctgaa atcctatgaa ccagaaaaat tatttgatgc acaaggacgt attttagaag
    17941 aaatcagaga aatcgcgcct actggtgacc aaagaatggc aaaaaatcct atcaccaatg
    18001 gtggaatcga tccaagaccg cttattatgc cagattggaa aaaatatact ttgcaatttg
    18061 aaaaaccagg ctccgttaaa gcagaagata tgaccgaact tggtaaattc gttcgcgaga
    18121 tcattgagaa aaatccagaa aatttccgta tatttggacc agatgaaaca aaatcaaacc
    18181 gattgaatca agttttcaaa acgacaaatc gccaatggat ggaaaaaatc gaaccagaaa
    18241 atgatgaatg gctttcgcca tctggtcgcg tcatcgacag tcaattatcg gaacaccaag
    18301 atgaagggtt cttagaaggg tacgttctga ctggacggca tggttttttg caagttacga
    18361 atcattcttg cgagtggttg actctatgtt gactcagcac ttcaaatgga tgcggaaatc
    18421 acatgacctt tcttggcgaa atgattatcc ttcgctaaat ctcattgcaa gctcaacagt
    18481 cttccagcaa gatcacaatg gatatagcca ccaagatcca ggaatattga cacatttggc
    18541 agaaaagaag gcggagttca tccgtgaata tctgcctgcg gatgcaaata cattgctcgc
    18601 agtcatggat aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct cttccaaaca
    18661 tccgcgtgct caattttact cagcagaaga agcagccgtc ttagtgaatg aaggcttgaa
    18721 aatcattgat tgggcttcga cagcaaaaga ggaggaacct gaattggtca ttgctgctgc
    18781 aggaacggaa tctaacttag aagcattagc agctgtaact ttgctgctgg aagaattccc
    18841 aaaactaaaa attcggttca tcaatgtcgt agatttattg aaattacgtc atccaagtca
    18901 agatccgcgt gggttgagcg atgaagaatt tgatcaatat tttacaaaag acaaaccgat
    18961 cttatttgct ttccacggtt atgaaacatt ggtccgcacg attttctttg atcgtcataa
    19021 ccatcattta atgattcatg gctacaaaga aaatggagat atcacgacac catttgacat
    19081 gcgtgtagta aatgaacttg atcgttatca tttggcaaaa gacgcagctt taaaaatcaa
    19141 aggaagtcaa gcagaagatt ttgcgaaaaa aatggatcag aaattacaag aacatcaaaa
    19201 ctatattcgt gaaaatggta ttgatctgcc agaagtattg gattggaaat ggaaaaatct
    19261 tgaccaataa aatgataaaa tcgatcccgc aaaatagaat tttttacatt gattaagaaa
    19321 ctgaaaaaat aaataaaaaa aacggaaaaa agactggaag aatggccagt cttttttttc
    19381 gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat ccaatcaaat
    19441 ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaagcataa catgatttct
    19501 atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa ccgttcatta
    19561 tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt aaatcaaaaa
    19621 tgaaatggag aaaagacatg gacgggttat tacctttatg gaaagaacgc ggaatgacaa
    19681 gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc ggacatgggg
    19741 gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa gccacaaaag
    19801 ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact ctaggtttca
    19861 gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta gatatgtctt
    19921 tatctgaaaa aaaaatagat gaagtgatgg cttcatttat tggtgagatc acccagatac
    19981 caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat gcccgcaata
    20041 atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc cggacaagtg
    20101 aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta gaatgcggga
    20161 aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt taccctgcgc
    20221 acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaggat gctctgacat
    20281 tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg ctgccaatag
    20341 aaaaaggagt ggaacggttc aaacgagtag atattactga tgaagtatgg cagaaagtaa
    20401 aaaacggtat gagacttgat tatactgtat ttgcgctccc agaaatgccg tcagaagaaa
    20461 ttgctttgtt ttataaagga aaagttgtca gtatttatca gccaaacccg aaagaaaaaa
    20521 acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat atcatcaaga
    20581 tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta gtgctgggat
    20641 ttttcgacgg cgtgcatatc ggacaccaga aagtcataaa gacaggtatg gaaattgcac
    20701 aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatct attgtcttta
    20761 aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa gaagaaaaaa
    20821 tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct tttgctcatt
    20881 tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa tatgctgttt
    20941 ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat tttcctgaat
    21001 atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa ggtgaaaaaa
    21061 tcagctctac ccgtattcgt ggattactag acagcggaca agtaaaagaa gcagcaaaac
    21121 tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca cgaggcagac
    21181 tactaggatt ccctacagca aatgtcaaag taaagagcac ggttcatttg cctaaagaag
    21241 gtgtttatgt gtcggaaata aaaattgggg aaacatggta tccagccatg ggttcaattg
    21301 gacataatga tacattcggt gaaggacggg agctgactgt agagttgtat attttggact
    21361 ttaatcaaga tatctatggt gaacatgtcg cggtacgctg gaatcaattt ttaagagatc
    21421 aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaggaggat gaacaagcaa
    21481 cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt ttatgtataa
    21541 ctagcaatta caataaaatg ggtgataaat aagcccagga aaggggctgt gacaaaagtc
    21601 ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt cggaaataag
    21661 ccgaaatttc ttgaagctga ccacttctgt cacaacctct ttctttatag aaataaaagg
    21721 acactagcaa ttcatgaaaa cgattgctag agcgtatcat ttgctttaga ctatatatct
    21781 tggtgtacac tcactttaag gcggaaataa aagttatccg taataacagg tttaggtatg
    21841 aaaaaggagt gggtactatg tacaaggaat tagaaaacaa agtcgcagta gtaactggtg
    21901 gctcaaaggg gattggaaca gcgattgctc aacgattcgg gaaagaaaaa atgaatgtag
    21961 ttgtcaacta taactcggat aaagaaggag cagaaaaagc agtcaaagca atcgaagagg
    22021 caggcggaaa agcagtctct gtacaagcag atgtcagttc tgaagaagga atacagcgtt
    22081 tacttgatac ggctttagaa aattttggga cactggacgt ttgggttaat aatgctggga
    22141 tggaaaatca gcatccaacc catgaactta gccttgaaga ttgggaaaga gtgttaaaag
    22201 tcaatttgac tggcgttttt atcggaacga aagcggcttt aaattacttt acagagaata
    22261 ataaaaaagg aaatatcatc aatatgtctt ctgttcatga acagatccca tggccaacgt
    22321 ttgctcatta cgctgcttca aaaggcgggg taaaattatt taccgaaacg gtagcaatgg
    22381 agtatgcacc gaagggaatc agaatcaata gtataggccc tggtgctatc cgtacaccta
    22441 ttaatgcggc aaaatttgat gatcctgaac aaaaagaact cttggaaagt atgatcccac
    22501 taggacgcgt aggtgaacca aaagaaatag cggcgacagc tgcatggttg gcttcagacg
    22561 aatccagtta tgttacaggt attacactat ttgcagatgg tgggatgact ttatatcctt
    22621 ctttccaagg tggaaaagga taagggtgaa taaaaatggg tttattgatt gcattgatcc
    22681 cggcagttgg ctgggggatc cagccactga ttttgaaaaa gattggtggc agaccaacaa
    22741 atgagatatt aggaactggt attggagcgg tgcttgtcgg cttactcgtt caattattcg
    22801 cttctccagc tggtatttca gtgactacat ttttgattag tttactttct ggtgcatttt
    22861 gggttattgg acaaacagga cagtatacat ctttcaactt gattggtgtt tctaaaacga
    22921 tgccaatttc gactgcatta cagttagtcg gaacctcgtt gattggtgtc ttcgcttttg
    22981 gagaatggtc tggaagtatg gggaaaatca ttggagcagc agcaatcatt cttcttgtat
    23041 ttggttctgc tttaacggca atcagtgatg atcctggaaa aaaaggcggt atcacaaaag
    23101 gaattagtct attagcatca acaagtattg gttattgggt atacagtgca ttgcctaaac
    23161 tagttgatgc aagcggactt tccattttct ttccgcaaat gatcggtgtc tttttagggg
    23221 cagtgattta tgtgattttc cgtaaaccaa ccgcattttc tgaagacaag agttggaaag
    23281 caacgatcgt tggagccatc tttagtgtgt cagcattggc ttatattttt tcagcaaatg
    23341 aaaatggggt tgctactgca tatatcatta cacagctaaa cgtagttatc tctacattag
    23401 gcggtatgta catcctgcat gaaaataaga caccaaaaga aaaaaaatta acgattatcg
    23461 gattattgct gatcgtagta ggaagcatgg tcacggtctt tatttgagga taaagatggt
    23521 aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac agtaagtact tataaaaaaa
    23581 tagcgctaga atttccaaca tctctggaat ttctagcgct ttttaattgt ttaagataca
    23641 agggaaatat ctgtctggat caataatttt tcattgatcc attgactaaa gtttacgaca
    23701 taattgaagt taccaccgat tagtatctcc gcatgattgt ctttataaag atcgtattgt
    23761 aaaagacggt cttttttatt tgcgtaaaca tcgttatggt actggactaa ttgttctcgg
    23821 aattgattga ttgctgtaaa atcacgatat tgaaggttga aacgcatcgt gattggtgca
    23881 tttagcatga atcgctgcat ttcttttgct gcttcctctg cttgatctcc gtataggaac
    23941 tcaaaaagat tacgttcaga aaatttccat tgcccgccaa cctttgttcc ttttagtttt
    24001 ccttcatgaa gataatttct gatagaacgt gtagtaacac ctaattgttc ggctaattca
    24061 tttactgagt aaaaatcttt caatgttcac cctcctatgt gaatggttct actgacttag
    24121 ttgatatttg cttaatcacg aaatgtagaa agaacgattt taattaaggt cattgtaaaa
    24181 atcaagtcag aaaattctaa cttttatttc tctaaagaaa ttcgttctct cctaaagtga
    24241 tattatattt aaatatacct gaaaataatg ggcagtatta tgaaagatca ctgatatttt
    24301 ctcgtatttt ttc
//
LOCUS       DARDHU010000032        23830 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000032 DARDHU010000000
VERSION     DARDHU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.032"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(54..788)
                     /locus_tag="SDD03_001321"
     CDS             complement(54..788)
                     /locus_tag="SDD03_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG4303771.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="SDD03_001322"
     CDS             1115..1525
                     /locus_tag="SDD03_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG4303772.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="SDD03_001323"
     CDS             1518..2219
                     /locus_tag="SDD03_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG4303773.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="SDD03_001324"
     CDS             2219..3805
                     /locus_tag="SDD03_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303774.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="SDD03_001325"
     CDS             3824..4045
                     /locus_tag="SDD03_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG4303775.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="SDD03_001326"
     CDS             4042..4803
                     /locus_tag="SDD03_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG4303776.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="SDD03_001327"
     CDS             5183..5692
                     /locus_tag="SDD03_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG4303777.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="SDD03_001328"
     CDS             complement(5767..6306)
                     /locus_tag="SDD03_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG4303778.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="SDD03_001329"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="SDD03_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG4303779.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="SDD03_001330"
     CDS             7319..8170
                     /locus_tag="SDD03_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG4303780.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="SDD03_001331"
     CDS             8198..8833
                     /locus_tag="SDD03_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG4303781.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="SDD03_001332"
     CDS             8853..9626
                     /locus_tag="SDD03_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4303782.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="SDD03_001333"
     CDS             10034..10831
                     /locus_tag="SDD03_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG4303783.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="SDD03_001334"
     CDS             10809..11222
                     /locus_tag="SDD03_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4303784.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="SDD03_001335"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="SDD03_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG4303785.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="SDD03_001336"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="SDD03_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG4303786.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="SDD03_001337"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="SDD03_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG4303787.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="SDD03_001338"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="SDD03_001338"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG4303788.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="SDD03_001339"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="SDD03_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG4303789.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="SDD03_001340"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="SDD03_001340"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG4303790.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="SDD03_001341"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="SDD03_001341"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG4303791.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="SDD03_001342"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="SDD03_001342"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG4303792.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="SDD03_001343"
     CDS             21906..22484
                     /locus_tag="SDD03_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4303793.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="SDD03_001344"
     CDS             22536..23018
                     /locus_tag="SDD03_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG4303794.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="SDD03_001345"
     CDS             23135..23509
                     /locus_tag="SDD03_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303795.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6649 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DARDHU010000033        23789 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000033 DARDHU010000000
VERSION     DARDHU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.033"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            384..2045
                     /locus_tag="SDD03_001346"
     CDS             384..2045
                     /locus_tag="SDD03_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4303796.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2115..2462)
                     /locus_tag="SDD03_001347"
     CDS             complement(2115..2462)
                     /locus_tag="SDD03_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG4303797.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2546..3487
                     /locus_tag="SDD03_001348"
     CDS             2546..3487
                     /locus_tag="SDD03_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303798.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3488..4531
                     /locus_tag="SDD03_001349"
     CDS             3488..4531
                     /locus_tag="SDD03_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303799.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4537..5607
                     /locus_tag="SDD03_001350"
     CDS             4537..5607
                     /locus_tag="SDD03_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303800.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5597..6550
                     /locus_tag="SDD03_001351"
     CDS             5597..6550
                     /locus_tag="SDD03_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4303801.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6562..7572
                     /locus_tag="SDD03_001352"
     CDS             6562..7572
                     /locus_tag="SDD03_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4303802.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7857..8678)
                     /locus_tag="SDD03_001353"
     CDS             complement(7857..8678)
                     /locus_tag="SDD03_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4303803.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8775..9053)
                     /locus_tag="SDD03_001354"
     CDS             complement(8775..9053)
                     /locus_tag="SDD03_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303804.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9321..11330
                     /gene="metG"
                     /locus_tag="SDD03_001355"
     CDS             9321..11330
                     /gene="metG"
                     /locus_tag="SDD03_001355"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG4303805.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11809..12396)
                     /locus_tag="SDD03_001356"
     CDS             complement(11809..12396)
                     /locus_tag="SDD03_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303806.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12571..13893
                     /locus_tag="SDD03_001357"
     CDS             12571..13893
                     /locus_tag="SDD03_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4303807.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14048..14824
                     /locus_tag="SDD03_001358"
     CDS             14048..14824
                     /locus_tag="SDD03_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG4303808.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14817..15386
                     /gene="rnmV"
                     /locus_tag="SDD03_001359"
     CDS             14817..15386
                     /gene="rnmV"
                     /locus_tag="SDD03_001359"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG4303809.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15379..16263
                     /gene="rsmA"
                     /locus_tag="SDD03_001360"
     CDS             15379..16263
                     /gene="rsmA"
                     /locus_tag="SDD03_001360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG4303810.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16714..17343
                     /locus_tag="SDD03_001361"
     CDS             16714..17343
                     /locus_tag="SDD03_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303811.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17467..18576)
                     /gene="ugpC"
                     /locus_tag="SDD03_001362"
     CDS             complement(17467..18576)
                     /gene="ugpC"
                     /locus_tag="SDD03_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG4303812.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18785..19207
                     /gene="mgsA"
                     /locus_tag="SDD03_001363"
     CDS             18785..19207
                     /gene="mgsA"
                     /locus_tag="SDD03_001363"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG4303813.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19530..20066
                     /locus_tag="SDD03_001364"
     CDS             19530..20066
                     /locus_tag="SDD03_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG4303814.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20106..21869)
                     /locus_tag="SDD03_001365"
     CDS             complement(20106..21869)
                     /locus_tag="SDD03_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303815.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21872..23587)
                     /locus_tag="SDD03_001366"
     CDS             complement(21872..23587)
                     /locus_tag="SDD03_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303816.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7675 a         4342 c         4822 g         6950 t
ORIGIN      
        1 aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag
       61 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
      121 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
      181 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
      241 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
      301 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
      361 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
      421 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
      481 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
      541 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
      601 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
      661 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
      721 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
      781 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
      841 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
      901 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
      961 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
     1021 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
     1081 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
     1141 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
     1201 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
     1261 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
     1321 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
     1381 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
     1441 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
     1501 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
     1561 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
     1621 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
     1681 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
     1741 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
     1801 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
     1861 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
     1921 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
     1981 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
     2041 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
     2101 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
     2161 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
     2221 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
     2281 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
     2341 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
     2401 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
     2461 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
     2521 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
     2581 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
     2641 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
     2701 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
     2761 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
     2821 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
     2881 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
     2941 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
     3001 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
     3061 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
     3121 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
     3181 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
     3241 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
     3301 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
     3361 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
     3421 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
     3481 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
     3541 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
     3601 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
     3661 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
     3721 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
     3781 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
     3841 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
     3901 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
     3961 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
     4021 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
     4081 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
     4141 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
     4201 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
     4261 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
     4321 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
     4381 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
     4441 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
     4501 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
     4561 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
     4621 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
     4681 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
     4741 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
     4801 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
     4861 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
     4921 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
     4981 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
     5041 ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact
     5101 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
     5161 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
     5221 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
     5281 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
     5341 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
     5401 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
     5461 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
     5521 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
     5581 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
     5641 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
     5701 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
     5761 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
     5821 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
     5881 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
     5941 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
     6001 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
     6061 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
     6121 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
     6181 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
     6241 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
     6301 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
     6361 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
     6421 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
     6481 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
     6541 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
     6601 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
     6661 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
     6721 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
     6781 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
     6841 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
     6901 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
     6961 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
     7021 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
     7081 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
     7141 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
     7201 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
     7261 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
     7321 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
     7381 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
     7441 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
     7501 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
     7561 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
     7621 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
     7681 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
     7741 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
     7801 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
     7861 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
     7921 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
     7981 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
     8041 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
     8101 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
     8161 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
     8221 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
     8281 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
     8341 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
     8401 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
     8461 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
     8521 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
     8581 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
     8641 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
     8701 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
     8761 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
     8821 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
     8881 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
     8941 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
     9001 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
     9061 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
     9121 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
     9181 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
     9241 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
     9301 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
     9361 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
     9421 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
     9481 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
     9541 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
     9601 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
     9661 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
     9721 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
     9781 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
     9841 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
     9901 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
     9961 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
    10021 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    10081 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    10141 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    10201 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    10261 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    10321 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    10381 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    10441 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    10501 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    10561 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    10621 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    10681 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    10741 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    10801 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    10861 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    10921 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    10981 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    11041 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    11101 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    11161 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    11221 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    11281 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    11341 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    11401 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    11461 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    11521 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    11581 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    11641 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    11701 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    11761 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    11821 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    11881 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    11941 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    12001 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    12061 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    12121 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    12181 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    12241 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    12301 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    12361 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    12421 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    12481 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    12541 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    12601 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    12661 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    12721 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    12781 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    12841 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    12901 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    12961 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    13021 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    13081 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    13141 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    13201 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    13261 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    13321 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    13381 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    13441 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    13501 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    13561 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    13621 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    13681 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    13741 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    13801 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    13861 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    13921 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    13981 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    14041 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    14101 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    14161 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    14221 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    14281 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    14341 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    14401 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    14461 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    14521 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    14581 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    14641 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    14701 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    14761 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    14821 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    14881 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    14941 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    15001 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    15061 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    15121 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    15181 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    15241 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    15301 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    15361 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    15421 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    15481 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    15541 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    15601 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    15661 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    15721 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    15781 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    15841 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    15901 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    15961 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    16021 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    16081 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    16141 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    16201 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    16261 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    16321 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttgttac cgcagatgca
    16381 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    16441 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    16501 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    16561 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    16621 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    16681 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    16741 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    16801 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    16861 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    16921 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    16981 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    17041 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    17101 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    17161 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    17221 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    17281 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    17341 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    17401 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    17461 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    17521 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    17581 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    17641 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    17701 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    17761 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    17821 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    17881 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    17941 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    18001 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    18061 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    18121 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    18181 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    18241 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    18301 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    18361 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    18421 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    18481 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    18541 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    18601 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    18661 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    18721 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    18781 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    18841 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    18901 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    18961 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    19021 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    19081 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    19141 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    19201 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    19261 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    19321 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    19381 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    19441 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    19501 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    19561 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    19621 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    19681 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    19741 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    19801 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    19861 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    19921 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    19981 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    20041 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    20101 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    20161 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    20221 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    20281 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    20341 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    20401 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    20461 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    20521 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    20581 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    20641 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    20701 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    20761 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    20821 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    20881 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    20941 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    21001 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    21061 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    21121 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    21181 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    21241 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    21301 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    21361 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    21421 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    21481 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    21541 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    21601 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    21661 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    21721 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    21781 atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    21841 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    21901 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    21961 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    22021 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    22081 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    22141 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    22201 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    22261 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    22321 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    22381 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    22441 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    22501 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    22561 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    22621 tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    22681 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    22741 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    22801 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    22861 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    22921 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    22981 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    23041 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    23101 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    23161 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    23221 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    23281 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    23341 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    23401 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    23461 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    23521 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    23581 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    23641 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    23701 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    23761 aacatgccag tctcttcatt cataggaac
//
LOCUS       DARDHU010000034        23619 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000034 DARDHU010000000
VERSION     DARDHU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.034"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="SDD03_001367"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="SDD03_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG4303817.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="SDD03_001368"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="SDD03_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG4303818.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="SDD03_001369"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="SDD03_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG4303819.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="SDD03_001370"
     CDS             4735..5628
                     /locus_tag="SDD03_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303820.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="SDD03_001371"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="SDD03_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG4303821.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="SDD03_001372"
     CDS             7565..8422
                     /locus_tag="SDD03_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG4303822.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="SDD03_001373"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="SDD03_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG4303823.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="SDD03_001374"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="SDD03_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG4303824.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="SDD03_001375"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="SDD03_001375"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG4303825.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="SDD03_001376"
     CDS             11178..11936
                     /locus_tag="SDD03_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG4303826.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="SDD03_001377"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="SDD03_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG4303827.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="SDD03_001378"
     CDS             12864..13895
                     /locus_tag="SDD03_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG4303828.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="SDD03_001379"
     CDS             13892..14659
                     /locus_tag="SDD03_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG4303829.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="SDD03_001380"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="SDD03_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG4303830.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="SDD03_001381"
     CDS             16624..16944
                     /locus_tag="SDD03_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG4303831.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="SDD03_001382"
     CDS             16944..17294
                     /locus_tag="SDD03_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG4303832.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="SDD03_001383"
     CDS             17461..18399
                     /locus_tag="SDD03_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG4303833.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="SDD03_001384"
     CDS             18414..19244
                     /locus_tag="SDD03_001384"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG4303834.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="SDD03_001385"
     CDS             19288..20313
                     /locus_tag="SDD03_001385"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG4303835.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="SDD03_001386"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="SDD03_001386"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG4303836.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="SDD03_001387"
     CDS             21391..21831
                     /locus_tag="SDD03_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG4303837.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="SDD03_001388"
     CDS             21834..22436
                     /locus_tag="SDD03_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG4303838.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="SDD03_001389"
     CDS             complement(22495..23601)
                     /locus_tag="SDD03_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG4303839.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         7738 a         4112 c         5149 g         6620 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttcta
//
LOCUS       DARDHU010000035        23063 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000035 DARDHU010000000
VERSION     DARDHU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.035"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..564)
                     /locus_tag="SDD03_001390"
     CDS             complement(109..564)
                     /locus_tag="SDD03_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303840.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="SDD03_001391"
     CDS             complement(718..1926)
                     /locus_tag="SDD03_001391"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG4303841.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="SDD03_001392"
     CDS             complement(2038..2370)
                     /locus_tag="SDD03_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG4303842.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="SDD03_001393"
     CDS             2518..3393
                     /locus_tag="SDD03_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4303843.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="SDD03_001394"
     CDS             complement(3475..4056)
                     /locus_tag="SDD03_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG4303844.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="SDD03_001395"
     CDS             complement(4062..5321)
                     /locus_tag="SDD03_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG4303845.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="SDD03_001396"
     CDS             complement(5323..6369)
                     /locus_tag="SDD03_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303846.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="SDD03_001397"
     CDS             complement(6604..6879)
                     /locus_tag="SDD03_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG4303847.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="SDD03_001398"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="SDD03_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG4303848.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="SDD03_001399"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="SDD03_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG4303849.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="SDD03_001400"
     CDS             complement(9978..10658)
                     /locus_tag="SDD03_001400"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG4303850.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="SDD03_001401"
     CDS             complement(10707..11342)
                     /locus_tag="SDD03_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4303851.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="SDD03_001402"
     CDS             complement(11408..12811)
                     /locus_tag="SDD03_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG4303852.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="SDD03_001403"
     CDS             complement(12798..13829)
                     /locus_tag="SDD03_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303853.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="SDD03_001404"
     CDS             13860..14081
                     /locus_tag="SDD03_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG4303854.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="SDD03_001405"
     CDS             complement(14246..14983)
                     /locus_tag="SDD03_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG4303855.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="SDD03_001406"
     CDS             complement(15136..16476)
                     /locus_tag="SDD03_001406"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG4303856.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="SDD03_001407"
     CDS             complement(16713..18236)
                     /locus_tag="SDD03_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG4303857.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="SDD03_001408"
     CDS             complement(18614..19225)
                     /locus_tag="SDD03_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4303858.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="SDD03_001409"
     CDS             complement(19555..20271)
                     /locus_tag="SDD03_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG4303859.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="SDD03_001410"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="SDD03_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG4303860.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="SDD03_001411"
     CDS             complement(20830..21627)
                     /locus_tag="SDD03_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG4303861.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="SDD03_001412"
     CDS             complement(21597..21968)
                     /locus_tag="SDD03_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG4303862.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="SDD03_001413"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="SDD03_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG4303863.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6466 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaa
//
LOCUS       DARDHU010000036        22825 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000036 DARDHU010000000
VERSION     DARDHU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.036"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..258)
                     /locus_tag="SDD03_001414"
     CDS             complement(<1..258)
                     /locus_tag="SDD03_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303864.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="SDD03_001415"
     CDS             complement(274..924)
                     /locus_tag="SDD03_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303865.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="SDD03_001416"
     CDS             complement(935..2107)
                     /locus_tag="SDD03_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG4303866.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="SDD03_001417"
     CDS             complement(2126..2998)
                     /locus_tag="SDD03_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4303867.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="SDD03_001418"
     CDS             complement(3020..3940)
                     /locus_tag="SDD03_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303868.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="SDD03_001419"
     CDS             complement(3940..4209)
                     /locus_tag="SDD03_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303869.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="SDD03_001420"
     CDS             complement(4206..4586)
                     /locus_tag="SDD03_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG4303870.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="SDD03_001421"
     CDS             complement(5053..6516)
                     /locus_tag="SDD03_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG4303871.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="SDD03_001422"
     CDS             complement(6580..9597)
                     /locus_tag="SDD03_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4303872.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="SDD03_001423"
     CDS             complement(9748..10587)
                     /locus_tag="SDD03_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4303873.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="SDD03_001424"
     CDS             10732..10977
                     /locus_tag="SDD03_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303874.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="SDD03_001425"
     CDS             11040..11462
                     /locus_tag="SDD03_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303875.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="SDD03_001426"
     CDS             complement(11556..11873)
                     /locus_tag="SDD03_001426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303876.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="SDD03_001427"
     CDS             complement(11900..12106)
                     /locus_tag="SDD03_001427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303877.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="SDD03_001428"
     CDS             complement(12087..12269)
                     /locus_tag="SDD03_001428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303878.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="SDD03_001429"
     CDS             complement(12283..12633)
                     /locus_tag="SDD03_001429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303879.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="SDD03_001430"
     CDS             complement(12659..12961)
                     /locus_tag="SDD03_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303880.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="SDD03_001431"
     CDS             complement(12964..13197)
                     /locus_tag="SDD03_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303881.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="SDD03_001432"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="SDD03_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="SDD03_001433"
     CDS             complement(14628..15743)
                     /locus_tag="SDD03_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG4303882.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="SDD03_001434"
     CDS             complement(15749..16147)
                     /locus_tag="SDD03_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG4303883.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17176..17562)
                     /locus_tag="SDD03_001435"
     CDS             complement(17176..17562)
                     /locus_tag="SDD03_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG4303884.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17559..18080)
                     /locus_tag="SDD03_001436"
     CDS             complement(17559..18080)
                     /locus_tag="SDD03_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4303885.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="SDD03_001437"
     CDS             complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="SDD03_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG4303886.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19149..20564)
                     /locus_tag="SDD03_001438"
     CDS             complement(19149..20564)
                     /locus_tag="SDD03_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG4303887.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20533..22686)
                     /locus_tag="SDD03_001439"
     CDS             complement(20533..22686)
                     /locus_tag="SDD03_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG4303888.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
BASE COUNT         6408 a         4623 c         3433 g         8361 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    16921 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    16981 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    17041 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    17101 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    17161 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    17221 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    17281 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    17341 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    17401 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    17461 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    17521 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    17581 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    17641 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    17701 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    17761 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    17821 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    17881 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    17941 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    18001 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    18061 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    18121 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    18181 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    18241 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    18301 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    18361 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    18421 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    18481 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    18541 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    18601 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    18661 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    18721 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    18781 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    18841 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    18901 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    18961 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    19021 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    19081 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    19141 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    19201 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    19261 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    19321 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    19381 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    19441 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    19501 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    19561 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    19621 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    19681 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    19741 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    19801 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    19861 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    19921 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    19981 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    20041 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    20101 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    20161 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    20221 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    20281 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    20341 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    20401 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    20461 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    20521 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    20581 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    20641 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    20701 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    20761 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgccctcc
    20821 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    20881 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    20941 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    21001 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    21061 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    21121 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    21181 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    21241 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    21301 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    21361 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    21421 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    21481 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    21541 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    21601 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    21661 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    21721 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    21781 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    21841 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    21901 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    21961 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    22021 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    22081 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    22141 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    22201 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    22261 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    22321 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    22381 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    22441 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    22501 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    22561 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    22621 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    22681 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    22741 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    22801 cactaacttt ttcattcctg aatca
//
LOCUS       DARDHU010000037        22732 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000037 DARDHU010000000
VERSION     DARDHU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.037"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(29..1291)
                     /locus_tag="SDD03_001440"
     CDS             complement(29..1291)
                     /locus_tag="SDD03_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303889.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1284..2183)
                     /locus_tag="SDD03_001441"
     CDS             complement(1284..2183)
                     /locus_tag="SDD03_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303890.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2328..4106)
                     /gene="uvrC"
                     /locus_tag="SDD03_001442"
     CDS             complement(2328..4106)
                     /gene="uvrC"
                     /locus_tag="SDD03_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG4303891.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4288..4602)
                     /gene="trxA"
                     /locus_tag="SDD03_001443"
     CDS             complement(4288..4602)
                     /gene="trxA"
                     /locus_tag="SDD03_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG4303892.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4689..7049)
                     /locus_tag="SDD03_001444"
     CDS             complement(4689..7049)
                     /locus_tag="SDD03_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG4303893.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7164..7711)
                     /locus_tag="SDD03_001445"
                     /pseudo
     CDS             complement(7164..7711)
                     /locus_tag="SDD03_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7769..8206)
                     /gene="zapA"
                     /locus_tag="SDD03_001446"
     CDS             complement(7769..8206)
                     /gene="zapA"
                     /locus_tag="SDD03_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG4303894.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8408..9325
                     /locus_tag="SDD03_001447"
     CDS             8408..9325
                     /locus_tag="SDD03_001447"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG4303895.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9581..10492)
                     /locus_tag="SDD03_001448"
     CDS             complement(9581..10492)
                     /locus_tag="SDD03_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4303896.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10895..11473)
                     /locus_tag="SDD03_001449"
     CDS             complement(10895..11473)
                     /locus_tag="SDD03_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4303897.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11493..12161)
                     /locus_tag="SDD03_001450"
     CDS             complement(11493..12161)
                     /locus_tag="SDD03_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4303898.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12176..13246)
                     /locus_tag="SDD03_001451"
     CDS             complement(12176..13246)
                     /locus_tag="SDD03_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4303899.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13417..14781)
                     /locus_tag="SDD03_001452"
     CDS             complement(13417..14781)
                     /locus_tag="SDD03_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4303900.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14778..16337)
                     /locus_tag="SDD03_001453"
     CDS             complement(14778..16337)
                     /locus_tag="SDD03_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303901.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16393..18873)
                     /locus_tag="SDD03_001454"
     CDS             complement(16393..18873)
                     /locus_tag="SDD03_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303902.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18857..19192)
                     /locus_tag="SDD03_001455"
     CDS             complement(18857..19192)
                     /locus_tag="SDD03_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303903.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19206..20240)
                     /locus_tag="SDD03_001456"
     CDS             complement(19206..20240)
                     /locus_tag="SDD03_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG4303904.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20330..21010)
                     /locus_tag="SDD03_001457"
     CDS             complement(20330..21010)
                     /locus_tag="SDD03_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4303905.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21294..22397)
                     /locus_tag="SDD03_001458"
     CDS             complement(21294..22397)
                     /locus_tag="SDD03_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG4303906.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         6485 a         4780 c         3862 g         7605 t
ORIGIN      
        1 ccagcttcct tctatatttt tctatatttt atgctttttt tcgttcattt ctcataattg
       61 aaatcgattg tttcaatgaa ctccataatc cgccttcgct atacacgagt acattcgatt
      121 tgtacatgtt tgcagacaag atcatcaata agaatgttac ggctacaagt accaacaagg
      181 aaatcaatgc tccacccata ccgactgtct catttgctaa gcgaaccggc ataatatatg
      241 aagataagaa tggaatatac gaagtgatcc gaataacgat attgtttgga tctgaagctc
      301 ctagtatcaa tccaagcata tatccgccta gagatagata agtcactggc aaaattgctt
      361 ttgctgtatc ttctgctttg ttgaccaaag agccacataa agctgctaaa acagaaaaaa
      421 tcaaaatgcc aaagatcatg aatagaagag aataccacaa gaatggtcca atcaagttat
      481 ccaatgagat accgtctaat atatttttga ccatttccat atctttgaat tgactgtatc
      541 ctataccaaa aataataccg tataatgcca tttgcgttaa tgcaacaagt aaaacacctg
      601 ttagttttcc gtaaaaatgc ttttgtgcag tcgtactgga caggatcact tccatgatcc
      661 gcgtgccttt ttcagaagcg atctcttgtg caatgatctg cgcatacgtc aaaataataa
      721 tgaacaagac aatcgttgct acataactta cgacatattg gactgtcgag ttatcttctc
      781 caactgtcat ttttcctttt gaatcaaaat tgactttttg tcgagtcaaa cttgcttgtt
      841 ggctcaagct agctacttct tctggtgaaa tgcctaattg acttgctcga agcatcgatt
      901 gcaagccagt cagttgttgt tgtatcagta gttgtgtact ttgtcctaat gaattttcgc
      961 tataaagcat cccatttatc ttatcattct cagttgagac tactaaatac gcatcgattt
     1021 tttcttccga aagctctttt tgtgcttttt cttcagaatc aactgctttg aactcaaaat
     1081 catctgtttt gacttggctc atttgctgac tgatttgctc attatcagcc aatacgccga
     1141 ttttattgac atcactattt tgttgagcaa agtttcctgc tacataaatg atacccagca
     1201 tgataaaagg gactaatatc atgatcaaga aagaaatcga cttaacattt tttaaataga
     1261 catctttggt aataatccaa aacttacgca tcgatttcac ctgctttcaa tttgaaaatc
     1321 tcttccaaag ttggcggttg ctggttaaac ataggaatgt agccgaactg agtcgcacga
     1381 gtaaagattt ctttcccagc ttcaggatct tctaaggtaa tctctaacgt attgtcttca
     1441 tgaaggatca cttttttcac gccttcgatt tcttctactt cctgttggct taaaccagat
     1501 tccaagaata acttcgtacg gccaaatgat tcacgaatct catgtacttt tccattcaaa
     1561 accatttttc cgtttcgcaa catgatcaaa tgatcgcaga ttttttctac attatccata
     1621 ttgtggctag aaaaaatgac acatgaacca tgttttttca attccaatat tccatctttt
     1681 agcaattcag catttaccgg atctaacccg ctgaatggct catccaaaat gatcaattta
     1741 ggttcatgga ttaacgtggc gatcagctgg actttttgct gatttccttt agacaaagat
     1801 tttactttat ccgtcttttt cccttttacc tgaaattttt ccatccaaaa atcgattttt
     1861 ggttcaatct cttttcttga tttccctcta agttctgcaa aataaagcag ttggtcttga
     1921 atcgttactt tcggataaag tccccgttcc tccggaagat aaccgatgac atcataatct
     1981 ttctccccaa gatgatggcc attccataac acttctcctt ggtcttgtgt caaaaaatcc
     2041 aagatcaaac gaaaagtcgt tgtcttccca gccccgtttt ggccaattag ccccatgatt
     2101 tttccatcgg ggatttggaa agagacatga tcgacagctg tgtagttccc aaaagtttta
     2161 acaagatcct tgacttctaa catttcttca ctccttcttc aagaaatcac atatatcctt
     2221 agacaccaga cgtaagcgat ttattattta ccaacaaaac gtaaacgctc tttttgttgt
     2281 acataaaatg ataggacact ttactttctt tgtaaagtgc cctttcctca ttcctgatgt
     2341 aatttatcaa atacgttttg tgcgacattt ttcggcagtc cgatttcttg taattcttct
     2401 gtcgtggctg ctgtgatatt tttcaacgat ttgaactctt taagcagcat tttttttcgt
     2461 tttggtccta acctttcaat ttcatctaat cgggaagcaa aactgttttt actgcgtagc
     2521 tgccgatgga aagtgattgc gaatcgatgg acttcatctt ggattcgctg gagtaagaaa
     2581 aactctggtg aatttcgctc aagcggtact acagccaaat ctataccgaa tagcaactca
     2641 tttgttttat gtttatcgtt ttttgccatg ccagcaatag gaatatcgat tcctaattga
     2701 ttttctaaga catcttttgc cacatctact tgtccttttc ctccatcgat caagatcaaa
     2761 tcaggtaaag gcaacccttc gcgaatcact cgtgaatatc tgcgatagat gacttctctc
     2821 attgatgcat aatcatccgg tccgttgact gtttttattt tatatttccg atattctttt
     2881 cttgccggtt ttccatcaat gaatacgacc atcgcagcca caggattcgt ccccatgata
     2941 ttagagttat caaaagactc gatacgtatt ggggtcggga tattcatcgc gtttcctaaa
     3001 cgttcaactg ctccgatcgt ccgttcctgt tttctggcaa tcagatcaaa ccgttcattc
     3061 aaagcaactg aggcattctt attcgccaat tcaactaatt ttttcttttc tcctcgtttt
     3121 ggttgcagca ctttcgtagc taacaaagct tctacactgg gcttgtcgat cgtatcagga
     3181 atcaagactt ctttaggaat gaaatgctca ttttcctgat aaaactgacc gaggtaagtc
     3241 aagaaatctt cttctgcttc gttatagaaa ggaaacattg aaacatctcg ttcaattagc
     3301 tttccttgtc ttacgaaaaa cacttggaca cacatccatc ctttgtcaat cgcataacca
     3361 aatacatctc gatccagtaa atcagtattt gtcatttttt ggcgagtcat gattgtctca
     3421 atggcacgga tctggtctcg gtattctgca gctttttcaa actccatgtt ttcagcagct
     3481 ttttccattt tctgatggat ctctgactcg attttttcgt gtcctccgtt taaaaacttt
     3541 ttcacttcat caacgatttt cgtatagact ttcggatcta cgtcgaaata ataaggacac
     3601 agacattgtc ccaaatgata gtaaagacaa ggctccttcg tctgactagg tttgcatttt
     3661 ctcagaggaa ataatcgatc gaggatcttc tttgtctcat tcgccgcacc gacatcggga
     3721 tatggtccaa aatacagtgc tttgtctttt agtactttac gagtaatcac caaacgggga
     3781 tatttttcat tcgttatctt aaggaaagga taggttttat catcctttaa cataatgttg
     3841 tatttaggat catttttctt tatcagattg atctctaaga gcaatgcttc gatattcgat
     3901 tctgtaacga tgtattcaaa gtcttcgatt tcactaacaa gccgttccgt tttcgtatta
     3961 tggctgccag taaaatatga gcggacacga ttttttaata ttttggcttt gccgacataa
     4021 atgatcgttc catttttatc tttcatcaag taacagccgg gctgatcagg aagtaaagct
     4081 aatttgtttt tgattcgttc gttcatctgg tactcccctt catattcaat taaagttatt
     4141 atagcatgtc tgtcaaaaaa acaatccagc ctgacaacgt aattatactc aaaaaatatt
     4201 aatttgaata accttaactc aaccgcaaaa aaaccgagga cggaactgga ttcagtccca
     4261 ccctcagctt atttttgctg taagttttta taaatattga tcgatcattt gacgtagttg
     4321 ttcttttgaa tgaacaccaa cagctttttc tactacttga ccatcttttt tcaacattaa
     4381 tgttggaatg ctcatgatac cgaattgttg aggagtttct ggattttcat ccacatccat
     4441 tttagcaatt ctgaattctt cttcatcata ttcttgttcc aactgatcca aaatcggtgc
     4501 ttgcatacga catggaccac accaagttgc ccaaaaatca atcaatacaa gtcctttatc
     4561 tgtttcttca ttaaatgtct tatctgtaat tacttgtgac atatcaaaga cctccatttt
     4621 taaaatctat gattagtata gcatttctat acttggatag cgaaccatct gcttaccttt
     4681 agtaagcttt acttaaattt aacaattgtc gcaccatttc cgccttggtt tgccggtgca
     4741 aactcaaagc ttttgacgct ccgatggttt ttaagataat cagtaattcc cttgcgaagt
     4801 gcgcctgttc cttttccgtg gacaatagtc acttgaggat aaccagctag aatggcagaa
     4861 tccaaatatt gatctacctc acttagcgct tcttcgtatc gttttcctcg aaggtccaat
     4921 tgattcggca cgtgactact agattcagat cgtacagcat ggataactcg ttgtgtcggc
     4981 tctttttgag gagcaacagg cgtcagctcg tcttcggaca cattcatttt taatataccg
     5041 atttctactt gccactgatt cccgttttta cgtaaaagcg tccctctttg gccgtaagtc
     5101 gtaaccaaca cttcgtctcc cggtttcaac gttttttgtt ctttcgcctt cttcaatact
     5161 ttatttttct tcaaagtttc ttcttgatgt agatttgcta attgggtctt tgcatcgatc
     5221 aactgatgct ctttcacatt tccttgccca atctgctgtt gcattttacg aatatcagca
     5281 atgatttttt ctgctttttc ttccgcctct gacacgactt cgtttgcttt tttctttgct
     5341 ttttccattt ctttttcacg ttcttcaaag aaatagctgt aagcttcttt caaatcatcg
     5401 tgaagttctt gtgcctcaga aacaaaatga cgcatttcca gatattcggt ctctgccatt
     5461 ttccggcgat tctctaaatc tgtgatcatt tcgtttaaat cttggctctc atcattcatc
     5521 aactgcttcg cttcatcaat aacttctgta tcaagaccta atcggctaga gatttcaaac
     5581 gcattgcttc gtccaggaac gccaatcagc aaacgatacg ttggacttag tgtatccaca
     5641 tcgaactcca tgctggcatt aatcgtattt gcccgattgt atccataaac tttcaattca
     5701 ggataatgcg ttgttgccat cacataagca gactttttac caagatcatc taagattgcg
     5761 attgctagag cagcaccttc ttgcggatct gttcctgcac cgagctcgtc aaataatacc
     5821 agactttttt cattgacttt tgataaaata tctacagtgt tcgtcatatg agaagaaaag
     5881 gtcgatagac tttgctcaat cgactgttcg tctcctatat ccgcaaatac ttcctcaaaa
     5941 ataccgattt gactttcttc acctgcagga atcggtaatc ctgcttgccc catcagttgt
     6001 aacagaccta aagtcttcaa cgtgatcgtt ttcccacctg tgttaggtcc tgtaatgacg
     6061 atggcttgat agtcttttcc gattgtgatg tcattagaaa ctactttttc ctgatcgatc
     6121 agtggatgtc ttgcttgttt caagacgaca tgattgtctt cactgattcc cggcacgatg
     6181 gcttttactt ctttaccaaa tcgtgctttt gcattcatca gatccatttt accaatcaca
     6241 taagcattgt ggatgatttc ttgacgatgg ggaacaagtt cagcagaaag ttcacttaaa
     6301 atacgttgaa tctcatttcg ttcagcgatt tggtattgac gcaaacgatt attcaaatcc
     6361 accacttgtt ttggttcaat aaataaagtc tgtcctgaag cactttgatc gtggactaca
     6421 ccgccaaata caccgcggta ttcttgtttg actgggataa cgtagcggtc atttcgcatg
     6481 gtgatgatcg catcacttaa atactttgca tttttcccac ggacgatccc atctaactgt
     6541 tcacgaacag cttgttcact tcgccgaatg ttctgtctta tgctctttaa ctcaggagaa
     6601 gcgtcatcag tcacgcgtcc gtcttcatcg attgcttcct ttaaacggcg gctcaaaaca
     6661 gggatcgtga ctaactgatc tgcccacata tacaaacgtg caaattcgat ttcactgtca
     6721 gaaagatcat caatgaaacg attcaattcg cttgtcgtag acaatactct ggaaacttgt
     6781 gcaagctcca ctccattcaa gtctgctccg atctcgatcc gtttcatatg gggacggata
     6841 ttttcaagtt taggtatagg gatcccgcct cgcagacgtt gcactttcaa tccatcctca
     6901 gtttcttgaa gccagtttgt gatcgtttgt ttctcactca ctggtaccaa ttctgccagt
     6961 tcttcttttc cttgtgcagt cactacaaat tgtctgacca tctgtttgac tttttcaaat
     7021 tccaatgttt caaggattcg gttattcatt ttatcacctt atctttcact atgaaaaaag
     7081 agctgtgccg gacatacgct acgcagcctt cttaaataaa ctactctgta caaaaaacga
     7141 aatattttcc agcactattt tccttaattc aaaatattcg ttacccaaag ctcatacatc
     7201 ttgttggtaa gaaatggcgt tctctcaacg ataaaacgag cgaaactatg ctcgccgaat
     7261 tgattttgga ccacactaat ggaacaaagg caagtaactg caaaatcaaa aatatagtaa
     7321 cgtaagccat gattagtgac aggatgcctc ctgcaatcca gtctacctgt ctcaagatcg
     7381 gcacatacgt caatccatgg acaaaaaccc ccacaaatcg ggtaagcagc cagccaatta
     7441 gcaaaataat cagaaaagca gttcctgcat aaaaagcttc atctaggtga aaagcaaata
     7501 cctgatcaaa aaatgctaac tttgatgttg gggtgacagc tgggtatgga atgtataatt
     7561 ccaaatgact tgctaatggt ttgtaaaaat gctgggcagc aataaatgac aatacatatc
     7621 caatcgcgaa tatgatttgc aaagcaaacc cgcgacgtgc tccagaaaaa aacgcaatta
     7681 gcaataaaaa taaaatcagc aaactcagca tgctacgttc ctttctcttc taactttttt
     7741 aaatttttct tattttgata aaaggatctt aattagaaga tttttgttta atttgtcctt
     7801 ttcgtttctc gtttaaaatc tgttgtgctt ccacatgatt tttaatggta tgatctgctt
     7861 gaccattttc tttcaatact tcccgagctt cgttctcgat tgcttcaatg cgtttcacac
     7921 gattttcaag ctctgtaaat ttgatcattt ttttctttaa ttctgctgtt tcttcttcca
     7981 gctccaagat tctttcttgt ttcttcaatt gatccgaaag cgcattgaca gccatcaaga
     8041 ttgctgcttg ttcattatcc atttgtggtg ataattgttt caattctgct aactgttcat
     8101 tgatcagctt cgttacgata tccatatgat gcttggtttc ccgtccgata atagtatatg
     8161 tttggttggc aatcactgct ttgtagcgtg ttttttcgtg cgccatacga atcatttcct
     8221 cctatttgat gaaagtttta tttagtgtac catgaagttg gttctttcac cgttctccac
     8281 aaatattttt cacatactta actattatat gataaacttt aaaaaaatgc tatgtctcgc
     8341 aatctcttag aaaacgaaaa gtcgtgagtt gcggtatagt tttatgtata aaaacgagga
     8401 gaattttatg agcaacgtca ttatcaaagc aacaaacgac caacttagtc aaatgaaagc
     8461 atactatcgc tcttatttgt taaataaaca gattccttat actagctttg ctgcaaaaaa
     8521 agacgggacg accattaccg cttatacatc cggaaaagtg atgtttcaag gtacgaatgc
     8581 agcaatagaa gctgataaat ggggggaggc acaggataaa caagccaagc cagcaaaaac
     8641 agcctcttct ttgccaaaaa atatcagtca attatccgtt ttaggtagtg atgaggtcgg
     8701 aaatggcagc tattttggtc cgctaacagt atgtgctgct tatgtgaaac gtgatcagct
     8761 tgcagaatta cgtaaacttg gtgttagaga ttcaaaagag ttaaaagata gccaaatcat
     8821 ccagcttgct gacatcttaa aaaaaaccat cccctataaa ctgcttgtct tatctccaga
     8881 aaaatacaat gagatccaac caaaatacaa tgccgttcgg atgaaagtcg ctttgcacaa
     8941 tcaagcgatc catttgctgc taaaaaaaat cgagccagaa aaaccagaag cgattttgat
     9001 cgatcagttt acgccggaag caaattttaa aaaatatgcg cgtttagaaa aaaatcaatt
     9061 acaagaaaaa ctgtacttcg ttactaaagg agaacaatat catttagctg ttgctgctgc
     9121 ttctatcatc agtcgtgcca gctttttgga agagttacac aaagcatcag ctgaagctgg
     9181 aatcaccctt ccatcaggag ctggaacgaa atctgaccaa gtagcggcaa aactattgga
     9241 gaaaggcggc atgcctatgc ttgaaaaata tgccaaactt cattttgcca atacagaaaa
     9301 agctttgaag ctgctaaaaa aataatggaa ctgtgacaaa agtcatgtca cagtcccttg
     9361 ttccgaataa acggtgttct catgccaact cctcggtcac aagtcacaat agtcatcaaa
     9421 catgagaagc tgttttttga aattgctcct tgtgacgcgg agttaaacgg caggctcaca
     9481 acctcttcct taaacggtgt tcaaaagctt gcatctgcga taataagccc ggccaaacga
     9541 aaaatatgag gagctatttt tgtttggctg ggcttattta ttactgaaaa taagtggtca
     9601 atgcttgtgt caaacttgca gcaatctgct gctgataact gtctgttact aattccttta
     9661 aatcactatc attattcata taacccatct cgattaaaat agaaggttga agattttctc
     9721 gaagaacttg atagttccca gtggagattc ctcttgaatc cagaggcaga tcagtgaagg
     9781 agtcagcaat tgtctgtgct aatgtttcct cttgtccata gtaataatat gtcgtgattc
     9841 cagttgcttc attcgcatat tccgtagagt ctgcgtgtaa actgataaag acatctgcct
     9901 ttttttcttc actgtagtcg catatatctc caagctgtac taattcatct gtagtatggg
     9961 tcaaataaac cgttgctcca gcatcctcta gcgccttttt tacttttatg gctgtactta
    10021 atgttatatc tttttcttct gtttgttcat cattgcttaa tgccccagta tcatttccgc
    10081 catgcccagg atctaataca atcactgcat cagatagctc ttccggtttt gtttgtatat
    10141 tctttgtgac tttggttcca tcttcgaggg ctaaatatcc agtatagcca tcatttgtct
    10201 tgatctgata agcatttttt gtttcagaaa gaaactccgc cagctctccg ccgtttatag
    10261 ttgcaagtgg ggtagttagc gagtcatctg tatatagtgt acggtcattt tttccgattt
    10321 tgattaatgt ctcttgttct gtatcatcaa atgattcctt tgtctttgta accgtactgt
    10381 tcgtactgct gaccgtttgt ccaactatct ttttttgacc aaaaacatct atttgaaaac
    10441 agataataat gataatgatt gcaaatacaa aagctccaat tatttttttc attcttgact
    10501 ctcacctcgt agatccagac taagggtcaa aacttaaaat cagcttaagt acgcaaaaaa
    10561 ggggctgtaa aagatataac cgtagaaaat aacattactg ctaaaatgat ttttttcatt
    10621 tttggcatat cctgaaatct ccttttgact attcgtggtg attcatctac ccaataaata
    10681 gtttccttca ttctacaaac aattaatttc ttcgttatta aattaaaaca cttcctggaa
    10741 caccgacata ataaataaaa atataccctg ataatcatga gagaataaca aatttgtatt
    10801 tatgaaaaac tacctatata aaaaaaacaa gatataacga tcttcgtcat atcttgcttc
    10861 ttcacctaac tatttttctc cctatataac agctttacgc ttctcctaat acaccattca
    10921 aatataaatc gatcaattgc gccacattta gcggttcttc cacaggtctt ttcgaccgag
    10981 tgatgtaagg tgctaacact aagaaaaaat gccttgctgc gatttctttt gaaatcccat
    11041 ttcttagcac agattggttt gctgaaaaga tttcttcaaa aggttcacga taatttttct
    11101 tccatagaaa gaaaagatga tctcttgttt cctttgaaag attgctttca agatcgtgaa
    11161 gcatcagaaa caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg
    11221 ttacttgaaa tcccgtttcg cttgtttgaa cgatttctct caaagctttt ccaacatctt
    11281 ttaagtggtt ttctaaaact gccgtatata gtttttcttt atcaccgaaa taatgataga
    11341 gattgggttg ggtgatccca actttttgtg cgatctctct tgttgaagtg ccgagatatc
    11401 ccttggacat aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt
    11461 atcgatggac catttttcca cctgcttttt ggttattctt tttctttcaa tacaccatct
    11521 tccatcataa atattctatc gcatttgtca atcagccgtg tatcgtgagt aaccatgatc
    11581 gttgctttat ttttgtcttt tgattctttt gccaagatat cgactacttc tcgtgccttt
    11641 tctgaatcca aactagcagt tggttcatct gctagaataa tggacggatc attgtacaat
    11701 gcacgagcga ttgcgaccct ttgccgttct ccaccagata gttcttctgg atacttgttg
    11761 atcaacttat ccacgccaag ctgctcaaat aattcttttg cttctttttg acgatgttca
    11821 tgtttgatct tatctactaa taataactgc ttttttactg tcagaaaagg aaccagattg
    11881 gaagcttgca aaataaagcc aatttcttta aaacgtagtt taacgcgttc actttctttt
    11941 tgattactga attcttgatc attgataaat acagagccac tgctaggcgt ctgcaatccg
    12001 cctgccaaag tcaaaaaagt acttttacct gaaccagaag gaccgatgat tgcaacaaat
    12061 tcccctttat cgatactcat ggttgtttct ttcaatgctt taactacttg gtcgccatct
    12121 tggaaacttt tttctacttc tttaaattct attgctttca tgctcttcct ccttcttatc
    12181 caattgcctt caacggatcg atttttacga ttgtccgaac agaaaatagt gcgcctaaca
    12241 cagctactac cagcatcaac aagctaatga cgccaaaaaa cagccaattg ctttggaatg
    12301 gaaccgcatc aggtagaaca agggatgttc ccactgttcc taatagacca agaagaatac
    12361 caacaaatga taaaatgaat gtttgtgcca ctacagaacg cgcaataaat cctcctgtga
    12421 tcccttgtgc tttcattaca ccaaagatat taatcttttg catcgtcagt acataaatga
    12481 aaatcccgat cacaatagcc gcaataacga tcaaaaatcc gatcataaat ccaaaagtca
    12541 atacttgcgc attatatcca ggtaattcat tgatgaattt agaaattttg atctgttcta
    12601 aatcttcagg taaattactg attttacctc tgacaactat tgcgttaatc cgtgcatttt
    12661 cacttgtatc ttctttttcg aagcggatct cttggtaagc attgatggtt gtatagagaa
    12721 ctggtgagac attgaatttg gcatgatccg taaagccagt tagtttaaac gtcttgtcac
    12781 taccagataa cttaactgta tctcctacgg ctagaccata ttcttctttc aaactaatat
    12841 cgcctactgc ctcatcatcg ttatcaaaag cttttccttc aacaagattg ggcatgatga
    12901 attgattttt atcgataccg aagaagctaa cattgatttt tccttcttct gtactgtctt
    12961 tactagtcac gactccaggt gtctgggcaa gatacgccac tttcccacct tcgacttctt
    13021 ctgctatctt cttagtaatc atggacatcc ctagatttga atttgcatct ttcgaaagca
    13081 caatcgaatc tgcctcccac ttgtcgactg ctgttcgatt atcttgagct aagccataag
    13141 ctaacccagt caaaaaaaat accaggtagg cgatcaaaaa catcacccct gcaactaacg
    13201 cataacgtaa ctttgaatgc ataatttcat ttaatgctaa aaacatttaa ctcctcctcc
    13261 tgaaataatc attttataca acaattttat cacttgataa atttttggcg aatattatgc
    13321 tcaaaaaatt cttccgctat ttcattatat gaatttttta ttctcgattg attaatattt
    13381 attatatccc cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt
    13441 gctatttgct ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga
    13501 ggtgtgttca gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg
    13561 tttcacgata tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg
    13621 tccgccaatt atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt
    13681 ttcttcatcg tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc
    13741 caagaatggc gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac
    13801 tgctgctgtt atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc
    13861 tactgctctt gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt
    13921 tttgtcaaaa ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg
    13981 cagatattca ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa
    14041 tgtcgtatta ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgcttgaga
    14101 tagaagttgt acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt
    14161 tggataaaaa ttgatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt
    14221 atcgaaatat cttgataaaa cattcggcat ataatcaact agatgaataa tgtatccttt
    14281 atttctcaaa gcatctgcta attccaatcc aatataacca gcaccgaaga tcactacttc
    14341 ttttgcttct tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta
    14401 gacattttca gcttcaattt gtttatacaa ggtgacagga gaggaacctg ttgctacgat
    14461 taatttatca taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg
    14521 tttgtctgga tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg
    14581 aagagtccgc atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag
    14641 gttgattcca tttgaaacat agcctagtcc atctttatga tcttcatcga tcagtaaaac
    14701 ttctgctttc ggttgaagtt ttcttagatt tagggcagct gtgatgcctg catgggaagc
    14761 accaatgatc acgattttca tgttttattc atccttaaat tcaatttttc taaataacat
    14821 tcttttaaaa attggttcaa ataggggaaa tcataattat attcgttgct agtgaataca
    14881 aaaactttag cggacttctg tgacgggtac tctttcaatt ggctagtcaa aatgatatca
    14941 tactgctttt ctttctcgta ttcgactaaa tcatatttga tcgtattcag caattgttcc
    15001 tggatgattt ctttcacgat atccgccatt agcctttcat aaggaagatc ataagcaatt
    15061 tgcagacgta attctaactg cttgatcagc gtccgcaaaa tagatgcata gcgagcaaat
    15121 aattcaaagc gggcattttc ctcgtctact tcagtaaaat caacaatagc aaatgtttgt
    15181 ctgaccaatc tttcacataa aaccaggaat tgtttatctt ctacttcagt taaccgctgt
    15241 tttaatcctt cttggccaaa aatattcatc catcctctaa aataggcaaa atgagcgtga
    15301 ttttgcatca ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac ataaaaagag
    15361 aaatattctt ctatcgattg tttaaacgtt cgattcaatt gagctactac gtcgtctttg
    15421 tatttttcgc ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta
    15481 ctgatcatga aaagataaaa aaccaacgtt tcattttgat tccaaggaaa ggcatattga
    15541 aagaagtaac gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat
    15601 ccaacaggaa gattgctata atctggtttg gattttttcg tacagcgcat tcgcttcttc
    15661 ataatggaaa gccatatttc aaatttcaat cgtccttctt ctgtaatagt aaaactaaac
    15721 tctttttgca gcagatctaa aaaagtgttt cctgataaat cactgtattc ttttttgttt
    15781 tcaagataag gacgtccaaa ccaaaataat tggaaataaa aataacaaat ttgactttct
    15841 ggaccggtca gccgcccgct ttttattttc agatgaaact catttaaaga atcatttaat
    15901 tccttcactc tgcgagacaa agtagccgaa ctgatattaa gttccatgac aagtttctgc
    15961 cgatcaaatg agccattttc caacagataa agaagtatct gatattttgt agaatagcgc
    16021 aagtagcaat gataataaac atcgactgct gctatatttc catgtctgat taataaaata
    16081 ttcccttctt ggataacaat ttccaattgt ttgatatttt ccaagttagc ttgtaaataa
    16141 aacagatctt ctttcaaggt agcccgatca actgataagc gggtaagtac gttattaacg
    16201 gaaatctctc ctttttcctc taataaaagt tggaaaagtt tgatttgccg ttgttcattt
    16261 ttttcaagca aactgaaaag ttccaagtcc actcctccaa gcatttctga ttttaacagc
    16321 agaagtttcc ttctcatcct tgaaaaagag gatctgtgac agttatcaca gatccataca
    16381 aattctaatc acttagctgg ttggcccagc tgtcagttcc catgtgacca tgccttgata
    16441 cgttgcgcca ggttgagcat tcgtactgct atttacctct aagagtaaac catcggtatc
    16501 gggaaagtca acgagaacct ctcctttagc tgttccatca ctctcataaa tcaacgtgtt
    16561 ttgaccttgc tgaatcaatt gttttgatcc agaatgattg aagtataatt ttgttccgct
    16621 taattcttga ccttgatcat tcttaaataa agaagtcaat gtccctgtca gttgccaagg
    16681 agacgtgctt ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt ttgcttcata
    16741 ataacttgtc ttcaacggtt tggggagatc gttaccaaat gtaaaatcat ctggtacatt
    16801 aactaatgtt aattcttgtt tggggacaag aattgtttta gcagatgttt ggacaactgg
    16861 gttaggaagc ttagcaccat ctgctaatgt aatattgctg gaaagtacag ctgcctttgt
    16921 cagtatgctg acggattgta cagaagtatc tacagtattt aattgatagt tgtaagtaag
    16981 atgaatggta tctcctattt tgctcaatag atctgctggg acagaaactc cttgttgaat
    17041 gccggtatct gcgatagttg taccatttaa ttttaaggaa ccattgatat aagtagtatt
    17101 ttcaggaagt gagatcgtga tttttttgtc ggttatcgca tccccagatt ttgttaacgt
    17161 gaatgtcaca ttttcttgaa tcgtatctcc tgatagagca gacaggggat ctcctgtctc
    17221 aggttgtctg ctaaggtttt ttagctgact atctgcactc acagatggaa aattagccac
    17281 tgttatttga ggaagagtgg tgattccagc ttgttcagac tgccatttgt acgcaaaaga
    17341 cggatacatg ttagtaagta catcgtccac caagcgataa tcaaaataat attgccctga
    17401 agccaatgca ggtagtgtag aagtagctgt atttacaccc ccgttatttg attggatcgt
    17461 ttggaaaagt tgatctgtcc atcctgtcgc taatttttgg gttcctttcg aataaagcct
    17521 gtaatggatc gaaccctttt ttagttcagt tgctgaccaa ttggccgtga cttggtacga
    17581 tgcagaagaa aggagctttc cccactgact atcggctgtt aagtttgttg gtaaaaccgt
    17641 tccgtctagc ttgtactgct ccaatacctt taaagcaaca ggagaaagca atccgccttg
    17701 cgcatctttc ccactgtaca cgatattggc tgtctgattc aatccacttg tgcttgctgg
    17761 agtatgagtc agtattgctc cttgtgtaac gttttttcct gtaaaatagt tgactacgcg
    17821 atcaaagcga ctaaatggca actgatcaaa acgtccttca gtcagcccca tcgtcaatgt
    17881 ccgactattg gtagagcctg gaaggttctg gttatttcca tccacgtatt ctgtggtatc
    17941 agcaaatgta ttatattttc ctgttgaatc acttgttgag atgtaactat tccacgtatt
    18001 tatctctgca cttgcagaaa atgcatatgc tcctggattc aagtttccgc cagttaattt
    18061 aggataaaat ctacctgcag ctggagctaa aatcgaattg aacatacgat cttggataat
    18121 attattcgaa tcataaaaat caacagaaaa aacggccttt ttagacatag aaggttgtaa
    18181 tttacccttg gtcaaaattt tgtttggact cgtatcatat ttatctccgt tttctgtcat
    18241 cttcgcgaca ttagctgtaa catcaaattg aagcgggata tcgtccacgt tgaattcgcc
    18301 tcgcgtaata atagcaataa tatctgccaa tagagtattt gtatcccacg accccatccc
    18361 aaaacgcaag gtatggcttc ctgctgataa taaactaccg ctagtaacct tgatgggggc
    18421 accggcaata tacaagtagg cgttgttcca gtcaattgct tttaatacat cactgcttga
    18481 tacatgttcc ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag tgatattcac
    18541 caaatcaagt acatttgtta gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc
    18601 tttccaagga tcatttgaac catcgttcaa ataagtagca gtcaatttag gcaattggtt
    18661 agccccagtt agttggacaa gcttcccgcc attgactgca ctacgtccac catttggagg
    18721 tacgatattt tcatacgtgt atggatcata agaaatggca ggaggcgttg ctccaacagc
    18781 ttctgccgaa ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa aaaagaagga
    18841 gcgaaaaaat gaaaatttat tttgtagcaa catgttcatc ctccttttcc tttcttctcc
    18901 ataacaacaa ccacgcaaga agccataatt ctataccaat gatcaaataa agaaaacgga
    18961 tcttttcttc attcgtctcc gggaatacgc catgttgatt agattcttta taaatcggta
    19021 gctgtgtctg ctgttttgat gcgccattga ctgaaccact tggatgcgga actggactta
    19081 gatcataaaa tgtaatcgta cctgaacttt tggtatctgc ttggactggc tcttgccagc
    19141 tgaagaacag caaacttgca ataatccctc ccaataagat cgatgttttc aacgaaatca
    19201 cctcatcatg ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc
    19261 aaccataaca ctagaactgc agcaaccaat cctgcataaa gccatgggaa agattgttct
    19321 tgtactgcaa aaacggaatt ttcatttact ttttgtgctt tttctttttt gatcgtaaag
    19381 ttttgtgtaa atcgccattc attactgtcg cctgattttg ctatgatttc tgcgcggtaa
    19441 tcgccggctt ttgctttatc accagctaaa tcaagattca agtgaaaatt cgaatttggt
    19501 gcaactaaaa attcattaga cgtattttca atataaagct gattttcata aaagatcttg
    19561 ctcgtaaata tcaaattgct gataatccta ggctgaggat tttggaaacg aagctgaaca
    19621 gttggctttg cgttgacctc gtccaaatct cctgataaaa gtttcagatc cggttctatc
    19681 gtctcttgac tttcttgtaa aacgactgcg agattataag caatacgatt tttgatttgt
    19741 gctccttctg tagcattttc tgtttcagct tcttctttag gtgaaacatg aatacccgct
    19801 aatacacgtc ccttaaagct ttttgctggg attttcactt ctataggtat agttgtttct
    19861 gcatgtgctg atacattgat ttcctttttc gcaatgagca aaacgtcacg gacatcaaac
    19921 ggcaatgtct catctaacgt tgggtttttt tgactataat cgatcgttcc accatcactc
    19981 gttaccgccg gattgacaga gacctcaaat gttcgttctt catctgaaga attgctgata
    20041 attacttcta gtgtctgatt ttgttccggt gccactaata aatcaaaata accgattgct
    20101 ttatctattt ggttctctgg aaataccggt ttgattccaa agtctcctgc tgaatccttt
    20161 gcttggctga ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac
    20221 agtcgtttac tttgattcat tgtccaccac atcctttcaa caacaactgc ggtaaaaaat
    20281 ccttcaccta tagtattttc accgcagttt gttgaattta tttcatactc tatgctactg
    20341 ggccagccac gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct gctttcggca
    20401 ctttcagtgt gatatttgct ggatcataaa cagaaagcca tgttcccaga ccttgatctt
    20461 ttgctgctgt aaatattggt gtcgctgcac ttgctttact caacccagtt acagtttgac
    20521 tagttggtcc agtggattcg ttatctgacg tacttttaac agtggatgtt ccaaaatcta
    20581 attcagcgcc ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt
    20641 tccacccttg tgcttggcca tctgcaccta cacctcgacg atctgatact tggagataag
    20701 gtgtatcgtt ttttgaagcg tctacttgat acgtttttgt tccgctttca atctcgtgtg
    20761 taccaaatgg caattcagga actacatcca atgttaaaga acctgtctgg ccagttgctg
    20821 gattttccgg atctgctggc tctactggtt ttgaaggatc agtagggtta agaggctcaa
    20881 cggtatttgt tggttcagta aaggtaatat ctccttttga agtcgcttga ccctcagcaa
    20941 aagctggtgt tgccaagccg cctagaagaa gtgttgaaag taataatgtc ccaatcatgt
    21001 tctttttcat aataaatcac tcctttttct tagtgaccta agtatagtag agacagtatg
    21061 caaaaaatcc tcatgaattc atccgcttac ttgaaattat gacccttttt gtataaaaga
    21121 gaaaaaaaca ataaaatcgt ttacatgaat attcttcctt ttttattaag tatgaaaaaa
    21181 tatttcaatt ttattttttc gtttcaaatt attctattag tgacatcctt gaactataaa
    21241 aataggagta cggcatcatc tgccgcactc ctattttttt cgtcatttat ttcttaaata
    21301 aaacgattct cacgcaattt tatttttttg atatcttcta ctgtttgtgt tccagctaat
    21361 tgcatgacca tttccaattc tgttttaaag aaatcaaaca cttgatggac tcctgtactt
    21421 cctcctaaag acaatccata aataacgggt ctgccaatag caacaagatc ggcacctgat
    21481 gcgattgctt tgaaaacatg ttgtccacgt cgtacaccgc tgtcaaacac gattggtaca
    21541 cgtttatcta ctgcttctgc cacatattgc aacgaatcaa acgcagcagg ccccccatct
    21601 aattgacggc caccatggtt agatacccaa attccaccag caccagattc taatgatcga
    21661 tagacatctt cttctgattg aacacctttc acgtagactg gcaaatcaga atgagcagcg
    21721 ataaattcta catcttttgg actaagtttt tgtttggaag atttatatac agcatccatc
    21781 gtttggccta ctcctgattg ataagcctga acgatcggca ttggcagcgg gaaggtaaac
    21841 ccgtttctgc ggtctgtttc acggtttccg ccaactgttg catccgctgt caatacgatt
    21901 gcttttgctc cattacgctt ggccacttca agaatatcca aattgattcc attatctttg
    21961 ctcatataaa attgaaacca ttgcggtgct ttctccccgc ctgcttctct tatctcttct
    22021 aaagtacaag aagcgtaaga acttgctgta tagatcgtcc caaaatctgc tacacctttt
    22081 gcagaagctt tctccgcctt cacatgagct aatccatgag cagcaactgg tgccatgatg
    22141 attggtgcag tcagcatttc ttcgtcaaaa tgtgtagttg tatcgggtaa ttctacatct
    22201 cttaatacat gaggaataat caaacgatga ttgaatgccc gttcattttc ttgataagta
    22261 aataagtcgc cagcaccact gctgatgtaa ccataaccac cagctggaat cacttgtgaa
    22321 gccgcgattt ctaaatcttc catattgata aaatctacta ttccttcatt tgtacctgct
    22381 tgatatgttt tttccatctc aatgccttct ctctataaaa tttgaatgat gaacaatatg
    22441 atagcgttta cactttttat attactcttc gacattgata caggcaaata atctgtttac
    22501 atttcagcca ttccagatgt cactactctg gaagaaatga ttgtaataaa aatagataaa
    22561 aaaccgatgg gactttgaca aaagtagtgt caaagtccct ttttttgaat aaacggtgtt
    22621 caaaagccat tttctcggta ttaagtcaaa tttatgcaaa aatgtggaaa gccattttca
    22681 caaaattttt cttactactc gaaactaagc gactttttca caacctctcg at
//
LOCUS       DARDHU010000038        22186 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000038 DARDHU010000000
VERSION     DARDHU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.038"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            54..1952
                     /locus_tag="SDD03_001459"
     CDS             54..1952
                     /locus_tag="SDD03_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG4303907.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2251..2970)
                     /locus_tag="SDD03_001460"
     CDS             complement(2251..2970)
                     /locus_tag="SDD03_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG4303908.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3307..3639
                     /locus_tag="SDD03_001461"
     CDS             3307..3639
                     /locus_tag="SDD03_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303909.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3626..5629
                     /locus_tag="SDD03_001462"
     CDS             3626..5629
                     /locus_tag="SDD03_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG4303910.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5648..6118
                     /locus_tag="SDD03_001463"
     CDS             5648..6118
                     /locus_tag="SDD03_001463"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG4303911.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6139..6723
                     /locus_tag="SDD03_001464"
     CDS             6139..6723
                     /locus_tag="SDD03_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG4303912.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6738..7724
                     /locus_tag="SDD03_001465"
     CDS             6738..7724
                     /locus_tag="SDD03_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG4303913.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7714..8025
                     /locus_tag="SDD03_001466"
     CDS             7714..8025
                     /locus_tag="SDD03_001466"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG4303914.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8047..9828
                     /locus_tag="SDD03_001467"
     CDS             8047..9828
                     /locus_tag="SDD03_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG4303915.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9821..11197
                     /locus_tag="SDD03_001468"
     CDS             9821..11197
                     /locus_tag="SDD03_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG4303916.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11204..11839
                     /locus_tag="SDD03_001469"
     CDS             11204..11839
                     /locus_tag="SDD03_001469"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG4303917.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11869..13224
                     /locus_tag="SDD03_001470"
     CDS             11869..13224
                     /locus_tag="SDD03_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG4303918.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13416..14810)
                     /locus_tag="SDD03_001471"
     CDS             complement(13416..14810)
                     /locus_tag="SDD03_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG4303919.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14902..15873
                     /locus_tag="SDD03_001472"
     CDS             14902..15873
                     /locus_tag="SDD03_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG4303920.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15870..16652
                     /locus_tag="SDD03_001473"
     CDS             15870..16652
                     /locus_tag="SDD03_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG4303921.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16821..17192)
                     /locus_tag="SDD03_001474"
     CDS             complement(16821..17192)
                     /locus_tag="SDD03_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4303922.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17326..17769
                     /locus_tag="SDD03_001475"
     CDS             17326..17769
                     /locus_tag="SDD03_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG4303923.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17812..18621)
                     /gene="yidA"
                     /locus_tag="SDD03_001476"
     CDS             complement(17812..18621)
                     /gene="yidA"
                     /locus_tag="SDD03_001476"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG4303924.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18640..20007)
                     /locus_tag="SDD03_001477"
     CDS             complement(18640..20007)
                     /locus_tag="SDD03_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4303925.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19991..20821)
                     /locus_tag="SDD03_001478"
     CDS             complement(19991..20821)
                     /locus_tag="SDD03_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG4303926.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20984..21421
                     /locus_tag="SDD03_001479"
     CDS             20984..21421
                     /locus_tag="SDD03_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG4303927.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21497..22117
                     /locus_tag="SDD03_001480"
     CDS             21497..22117
                     /locus_tag="SDD03_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG4303928.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         7116 a         3850 c         4783 g         6437 t
ORIGIN      
        1 tagagcataa caactttata atgttgagta taccaatttt tggaggcgtt ttaatggacc
       61 gctatttaga tttactttca gagaagttcc ctacaacgga agatgtcatc acggaaatca
      121 tcaatctcga agcaattttg cagcttccaa aaggaacaga gttatttttg agcgatatac
      181 atggcgaatt tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa
      241 agatcaaaga ccttttctct gaacaaatga cggaaacgga gatcagccaa ttcactattt
      301 ttacggctta tccagaatat gtgttgacga ctgattggta caaaaaacaa gataaatccc
      361 agctgatcca tcgtttgatc gatcttttac ggtttacgac agtcaaatac actcgatcaa
      421 aagttcgaaa aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg
      481 atgatcgtat caacgggaaa aaagactatg taaaaaaaat cattgaacaa cttgtcttga
      541 tgaaggaaga agaaaaattt ttaacaaaac ttgcgcagac gatccaaaaa tctgtcatcg
      601 atcatttgca tatcgtgggt gatatttttg atcgaggaac tcaagcagcc aaagtaatgg
      661 atcgaatcat ggatttttcc tctgtagata tccagtgggg gaatcatgat attttgtgga
      721 ttggtgcgta ttgcggctct gaggcatgtt tattgacgct tttacggatt gccgcacgct
      781 acaactattt atttgaatta gagaaagaat atggattaaa tttacgtcca ttattttctt
      841 ttgcccatca aacctatcag aagaatccag tatttacccc taaaaacagg aaagatctat
      901 ctgaaagaga gcaagaagaa ttagaaaaga tccatcaagc actttctatc ctccaattca
      961 aatcagaaca tcagttgttg aatcgacgac ctgaatttaa aatggacgat cgtaaattgt
     1021 tggaaaaaat caactatgag gaatcaacga tcgttttaga aggtcagctc tatgagttga
     1081 aagggacttg ttttcaaacg atccagccag atgatcctaa aaaattcttg gaagaggaac
     1141 aaaaagtgat cgatagtttg atgtattcat tccagcactc gttaagaatg caaaaacaca
     1201 tgacgttttt gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtactgttcc
     1261 atggctgtat acctgtagat gaaaaggggc agctcttagc atttgaatta gaccaatctt
     1321 atcgcggaaa agaactgctg ggattttttg aaaaacagat aactgaaagc gcaaaagatt
     1381 tgactgcaaa tgaagatttg gcgaccgatt tgatttggta tgcgtggagc gggccatttt
     1441 cgcctttatt cggcaagagt aaaatggcaa cttttgagcg ctattttctc acagagcctc
     1501 atacgcacgt cgaaaaaagc aatccctatt atcacttgcg agatgaagag tggttcagtg
     1561 aaaaaatttt ggcagaattc gatgcaaaag aagaaggttc agcaatcatc aatggacaca
     1621 cgccagtaaa agtaaaaaaa ggagagtcac cgatcaaagc aaacggaaaa gcatttgtta
     1681 ttgacggagg tctatccaaa gcttatcaaa aaacaaccgg gattgctggc tactcattac
     1741 tatgcaattc ttacggtttc caaatcgtga cacattatcc gttcttgcct attgaacaac
     1801 aatttgcaaa aaaatctgat caaacgaatg taaaaaaagt gatcgagcag cagctcccac
     1861 gcaaacttaa tcggaatacg acaaaaggaa tggaattaca gcaacagatt actcaactcc
     1921 aaaggctgtt ggattatcga aaggacgatt gagaaaaatg ctagccaaca ttgctcaata
     1981 gactaaaaag cgactgcaac aaaaggcgtg tcgcagtctc cttcggaatt agctcaaaaa
     2041 acggtaaaat attagaagct atttttctgt ttatttctct aatactcagg tcagggcgct
     2101 gatttcacga cctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
     2161 atatccaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttgaac
     2221 ggcaggctca cgacctaatc agaaaaacga ttaaaattca ttcacaaaga aatcgaataa
     2281 acgctgttca gatgaatcta cacgatgaga aagttccgga tgccattgga caccaaggat
     2341 tttgtgcata ttatctttac tttggactgc ttcaatgagt ccatcgttgg aaaaggcgat
     2401 ggcttttaac aaaggagaaa gatctttgac tgcctgatga tgataggaat tcacaaagta
     2461 tgaatcaggg agaaggtcac ttaataaact gtccgaaacg attttgactt cgtgagttgc
     2521 aaactgcggg gcagtgggct gttgttcgtg ttttacggac cattttggat ataatgaaag
     2581 atcttgataa agtgttccct ccaacacgac attcaatagt tgaagtcctc ggcagactgc
     2641 aaaaatcggt tttctttgtt tcaatgcttc caagatcaat gcctgttcga attggtctcg
     2701 ttgaatattt gtttccgtca gttttggatg agggtcttcc atgtaaagtt gcggactaac
     2761 atcctgaccg cctgcaagaa tgagtttgtc gattttctca attagttgag gcgctgaatc
     2821 tggagacatg actggcagaa taagaggtaa tccttgagca tctaaaactg catccacaaa
     2881 tccctgcggt gtatagctta cttgatttcc ttggaaagta tctgtcgctc gaatcagttg
     2941 attcccggca atgccgatga ttggtttcat ttttattccc cctaaagtag actaattgta
     3001 acaaggattt aaaaaaattt aaactatgta agacttgccc ctatacttat ctgttcttgc
     3061 tgtgttacta tctgtaaaga gaattttcgt gtgaaattca tgacgatagt tgaaaatatt
     3121 gcttaatttg ttataataaa aagtaagcag aaataaaaag gttgggcgta agcttcaagg
     3181 atctagttct tcaaggggtg agaaggacat tttattgaag gggcctgcct atactaagtt
     3241 atctttagag gtacatactt tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga
     3301 aaggaagtga agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga agaagaaaat
     3361 gagaagcgtg ctcgaaccct acggcagcag ttgaagacct atgcagaaga aaaagagcaa
     3421 gaattggaga taatccaaac gaacagtcgg caacagctga aacagttggt agaagaaaaa
     3481 gaacaattcg aaaagcaaga attagaaaaa gaaaaaatga aattgaaagc agaagcagac
     3541 aaggtgcaag caactttgaa agaccaatat caaaagcatc agagtcaagc aatcggtgca
     3601 atcattgaaa gggtgaaaga aacttatggc cgtcactaag atggaaaaag tgaccctcat
     3661 ttcagataaa agaaatcagg agaggatttt acaagcagta caaggaatcc aaaatgttga
     3721 aatccgggat ctgtatcaaa atagaacaaa caaccagtgg gtagaaaaat attttccaga
     3781 taccacgatt attgataaag aagcaaaatt aagcgcttta ggcaatcggc tgaaagacat
     3841 ccaagaagct atccaattca tacaacacca cggcgatagc cgtcaaaaga aactccattt
     3901 gaaacgtcaa gaactttcct tacaagactt ggaagcaaat tattccgaag aaaatttcat
     3961 gaataaatta gaagaagtcc ttgatttaaa agaaaagtgg gaacagctaa ctgaaaaacg
     4021 tgatcgttta gaagaagagg aagaatggct tcttcattgg caacagctgg atattgctcc
     4081 aagtatgtat gcaagtaaga cgaccatttt tgagatgggg actgtgagtg cagcaaattt
     4141 tgaaagtttc aaggaagaac taagtaagct tgatgacgtt tatatggaag aaatagatta
     4201 tacgccaaaa caagtttatc tttcgtatgt tgctttaaaa aaatcagttg ctgctataga
     4261 agaaattgct gggaaatacg ggttcaataa agaaacgtat ccttatgaac aaaatcctaa
     4321 agaaaaattg caagaaacca aacagcaatt atccattgtt cacgagcagc aaaaaaagat
     4381 aacgacagcg ttaggtcagt gcagcggcta tatccaagaa tttgaatggg tagaagaagt
     4441 cacacttgcc gttgcagaac gggaaaaagt aaaagaacgg tttatccatg ccacttatct
     4501 gatcgttttg caaggttggg tagatgcaga agaaaaacag aatttgatgc atgtgttaaa
     4561 cgaaactgta tcagaagaag atatatacgt tagtttcgaa acaccaaact cagaagagat
     4621 tcagcaggaa gtccctacta aattgaaaaa ccatccgctt atcgaacctt ttgaattgct
     4681 gactgaaatg tacagtctgc caaaatatga agaagtagat ccaacgcctt ggatgacacc
     4741 attttatctt gtcttcttcg ggatgatggt cgcagatgtt ggttacggat tgctgatgct
     4801 cattgggaca atactagctc aaaaactatt tgttttaccc cgcgggatga aacgtttcgt
     4861 aaaattcttt gagattctct cggtaccatc gatcatctgg ggattgatct acagttcatt
     4921 tttcggacag gcattaccaa aagaactatt tggtatccgc ttgcctttcc caatcctatc
     4981 tacgacggat gacgtcaata cgattttgat tttatccgtg attttcggtc tgatacagat
     5041 cttggttgga ttattcgtgg cagcaaagga aaatatcagg aggaaagatt acttgagtgc
     5101 tgtcagtgat ggttttgcct ggcaagggat cctgattggg atcgttattg cattgattgg
     5161 tgtcatgttg ataaaaaata cagcatttgt ttatcttggc gcggggctat cgatcattgc
     5221 tgctttatgt atcttaatcg tgccgattat ccaatcgccg tctaaagtca agggtgctgc
     5281 caaaggagcg tataatcttt acggattgac tggttatatc ggtgatttag tcagttatac
     5341 acgtttgatg gcactaggta tttcaggcgg aagtattggc gcggcattca atatgctagt
     5401 tgctttcatg ccgccagccg ctcgattcag tgtagggatc cttttgatca tcgctttaca
     5461 cgcactgaat atgtttttga cactgttaag tgcctatgtc catggtgcac gattgcagta
     5521 tgtcgaattt tttggaaaat tttatacagg aggcggacgt gcctttgacc cgctgaaaac
     5581 ggcggaaaaa tacgtcaaca tcaatcataa gaaaaaaaac aataaataat tggaggattt
     5641 ttatacaatg atggattact taattagtca aaatggtgga atggtatttg cagtattagc
     5701 gatggcaaca gcgacgattt tttcagggat tggttcagct aaaggtgttg gaatgacagg
     5761 tgaggcagca gcagcgttaa caacaagtca acccgaaaaa ttcggacagg ctttgattct
     5821 gcaattgctt ccaggtacgc aagggttgta tggattcgtt atcgcctttt tgatttttat
     5881 caacttagga aatgatatgt cagttgtaca aggattgaac tttctaggcg cttctctgcc
     5941 gattgctttt acaggtttgt tttcaggtat cgcccaaggg aaagttgcag cagcaggtat
     6001 ccaaatttta gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg ctgcgatggt
     6061 tgaaacatat gcgattttag gtttcgttat ttccttccta ttagtattga atgcctaagc
     6121 ataaaggagg aaaagcagat gaacgccatt gaaaaaatca tctctcagat gaatgaagca
     6181 gcagagcaag aaagagcggc gttagagcaa gaagagcgaa tgaagatcga tcaaaacttt
     6241 gaacaaaaac gtacgcaaat cgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa
     6301 ttacttgaaa aaaaataccg tcagcttagg aatcgccaac aagtagaagt acgacaagaa
     6361 aatttgaatg cgaagcagga atttttacga cgattgtttg cagatgcaat agctgaaatg
     6421 gagaattggg atgaatctga acaaatccag tttatcaaaa attcattgta ttcattacct
     6481 ttgacaggca aagtaacatt cattgctgga gaaaaatcag cagcttatct ttctcagaca
     6541 ttgctggatg aatggaacaa tgaacttccc tttatgatgg ttttaagtga tgaaactgtt
     6601 gctgatcaag cgggttttct gataaacgat caaggagttc agtataattt tcttttcagc
     6661 agccttgtac aagatatcca aggaacgatg agttttgaga ttgccaatca attattcgaa
     6721 taaggaggga tcagtgtatg gaataccacg aactaaatcc cttgattcgc ggaagagagc
     6781 ttgaactttt gtccaatgag acatttgaac ggatgattca aacaaattct gtcgaagcac
     6841 ttggtgaaat attgaaatcc acgatttaca gtccatatat ttatgacgga tatgaatatg
     6901 acttcgaaca gaatctttta aaggagcaga gccgtctatt tcgctggtta aaggaatcag
     6961 caccggagcc tgaaatcgtt tggatctata cgatgcgcta tactttccat aatctgaagg
     7021 ttttgacaaa agccgagatg acaaatcaaa atctggacca tttgtatatc gacgatggat
     7081 tttattcatt agaaaccttg aaggatgcca ttcatacgca agtttcaaat gaactccctg
     7141 aaagcattat ggacagtata cgagaagtac gtgattattt tgaagagtct accattcttc
     7201 agggaattga cgtgatttat gatcgacagt ttttgacaga gcagcgacgg cttgccgata
     7261 agctaggata tcaagaattg ctgacagaag tcattgcttt tattgacttg accaatatca
     7321 caacgatggc tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg acggtacttt
     7381 ccagctcggg aagtatcgaa aaagaagtat ttttgaactt tgtagataaa gatatggaag
     7441 attacacgaa ttttctatta gagacagatt atgctgattt tctgcgccct gtcatccgtg
     7501 agggaaatct tgatctcgta aagcttgaac agttgaaaga tgattatcta agttcattgt
     7561 atcaagaagc acaaacccag gctttcggac cactgccact actagccttt ttgaacgcaa
     7621 aagaggtaga aagcaaaaac cttcgtttac tggttatcgg caaaagaagc catttcgaac
     7681 cagaaacaat cagaaagcgg gtgagacaag tctatgacgc ataagatcgg tgtagttggc
     7741 gacaaagatt ctgttttgcc attcaaattg tttggctttg atgttcgtta tggtacaacc
     7801 aagcaaaccg ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg cgtgatttat
     7861 gtcacagagc aatgtgcagc attgagccca gaaacgatcg aacgttacaa ggaacaaatg
     7921 atacctgcta tcgtattgat tcccaatcat caaggatcat tagggatagg gctggaagaa
     7981 attcaaaaaa acgtagaaaa agcagtaggt caaaatatct tatagtatga aaaggagata
     8041 gaaattttgc aaattggaaa aatcataaaa gtctccggac ctcttgtaat ggctgagaac
     8101 atgtctgatg ccagtatcca agacatgtgt ctagtgggag atctaggagt tatcggagaa
     8161 attatcgaga tgcgtggcga tgtggcgtct attcaagtat atgaagagac ttctggaatt
     8221 ggtccaggcg aaccggttcg ctctactggt gaagctttat ctgttgaatt aggaccaggg
     8281 atcatttcac aaatgtttga cggaatccaa cggccattag atacctttat ggaaatcaca
     8341 caaagtaatt tcttagggcg cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg
     8401 tggttcgaac caacagtaga agccggagaa acggtgagtg ctggagatgt tatcgggatc
     8461 gtagaagaaa caaaagttat aaaacataaa atcatggtgc caaatgggat aaaaggaacg
     8521 atcaaaacaa tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt cattgaaacc
     8581 gaaaatggtg acaaaaattt gacgatgctt caaaaatggc cagtaagacg tagtcggcca
     8641 atcaaagaaa aattaaaccc ggatgcaccc atgatcactg gacagcgagt gattgataca
     8701 ttcttcccag tgacaaaagg aggagcagca gctgtacctg gtccttttgg tgcagggaaa
     8761 acagtcgttc agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga
     8821 tgtggcgagc gtggaaatga gatgacggat gttttaaatg aattccctga attgattgac
     8881 cctaatacag gagaatcgtt gatggaacgt acggtattga ttgctaatac atcaaacatg
     8941 ccagtagcag ctcgagaagc ttctatctat acgggaatca caattgcgga atatttccgt
     9001 gacatgggtt atgacgttgc tatcatggca gactccactt cacgatgggc agaagcactt
     9061 cgtgagatga gcggacgttt ggaagaaatg cctggtgatg aaggttatcc agcttatctt
     9121 ggttcacggc ttgcggaata ctatgaacga tctggaagag tcattgcatt aggatcagaa
     9181 ggtcgcgaag gaagtataac cgcaatcagt gctgtatccc cgtctggagg ggatatttcg
     9241 gaaccagtca cacaaaatac attgcgggtc gtaaaagtat tctggggtct tgattccagt
     9301 ttagcccaaa aaagacattt tccttcgatc gattggatac aaagctattc tctttattca
     9361 acagaagtag ggaagtatat ggatcatatc cttcaagctg actgggcatc catggtcacc
     9421 gagggcatgc gtattttgca ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg
     9481 attgattccc tttcagataa tgaccgtctg acattagaag tggcaaaatc cattcgtgaa
     9541 gattatttgc agcaaaatgc ttttgatgat gtcgatacat tcacttcaag agagaaacaa
     9601 ttcaaaatgt tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta
     9661 ggtgcttatt taagtgagat catggacggt accgtatcga ttcgtgaaag gatcggacga
     9721 agcaaatata tcccggaaac agaattagaa aaactagatc aaattattgg tgaaatcaag
     9781 caaacgatgc aaacgatcgt ttcagaagga ggcatgagca atgattaaag aatatcgtac
     9841 gatcggtgaa gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg tcaaatatga
     9901 agaattaatc gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag tattggaagt
     9961 ccaagaagat aaagcaatgg tccaaatatt cgaaggaaca agtggtatca atttacgtga
    10021 ctcttctgta cgatttttag gccatccttt agaactaggt gtatcggaag acatgatcgg
    10081 tcgagtattc gatggtttgg gacgaccgaa agataacgga ccagagatcc tgcctgaaaa
    10141 gcatttggat attaacggag aagtaatcaa tccagtagct cgggattatc cagatgagtt
    10201 tatccaaaca ggtatctctg caatcgatca tttgaataca ttagttcgtg ggcaaaaatt
    10261 accagtattt tctggctctg gtcttcctca taaagaatta gctgctcaga tcgcgcgaca
    10321 agcaacggta ctcgattcat cagatgattt cgccgtagtc tttgctgcta ttggaatcac
    10381 ttttgaagaa gctgaatttt tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc
    10441 tgtcatgttt atgaatttag caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat
    10501 ggctttgact gctgcagaat atcttgctta tgaaaaggga atgcatgttt tggttatcat
    10561 gacagacatg accaactatg cagaggcatt gcgagagatt tctgcagcac ggcgagaagt
    10621 tccaggaaga agaggatatc ctgggtatct ttacacgaac ttagctactt tattcgaacg
    10681 agcaggaaga atccgcggac taaaaggatc ggttactcaa attccgattc taacaatgcc
    10741 agaagacgat aaaactcatc caatccctga cttgactggt tatattacag aaggacagat
    10801 catcttgact agagagcttt acaagagtgg gattcagcca ccaattgatg tactcccgtc
    10861 tctttcgcgg ttaaaagata agggaactgg acccggaaag acaagagaag accatgcagc
    10921 tacgatgaat cagctttttg cagcctatgc acaaggaaaa caggcaaaag aattagccgt
    10981 agttttaggt gaatctgctt tatctgatgt agacaagatc tatgcgaagt ttgctgagcg
    11041 gtttgaaaaa gaatatgtca atcaaggatt ctacacgaac cgttcgatta ctgaaacact
    11101 tgatctaggg tgggaactgc tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga
    11161 cgacatgctg gacaaatatc tgccagaagg gaagtgagtt ctgatggcac gattgaatgt
    11221 gaatccgacc cgcatggaat tgactcgatt gaaaaaacag ttgacaactg ccacaagagg
    11281 gcataaatta ttaaaagata agcaagatga actgatgcgt caattcatcc tgttgattcg
    11341 aaagaacaac cagctgcgtc aagaaatgga agagtctatg acagaagcaa tgtctgattt
    11401 tgtattggca aatgcttcga tcacggaacc tttcatcgaa gaattatttg ttttgccggc
    11461 ggaaaacgtt gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat
    11521 gaattttgaa tatgatgaac agctgacaga cacacctttg gaatacgggt atttgaattc
    11581 cagtgccgaa ctggatcggt caatcgatcg gtttactacc ctgcttccaa aacttttgga
    11641 actgacagaa atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg
    11701 gcgagtcaat gcattagaat atatgacgat ccctcaatta gaagaaacga tctattatat
    11761 tcgtatgaag ctggaagaaa acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa
    11821 tatgggtacc actgactaag tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa
    11881 agcaaacaaa taaagaaacg tttgccggct gttcagcttc tcgcattagg attttttctc
    11941 ctgattttag ttggcggcgg cctacttacc ctaccatttt ttagtaatag tgggcatggg
    12001 acaaatttta tcgatgcgct tttcactgca acttctgctg tctgtgtaac gggattgaca
    12061 acattgaata cagcagagca ttggaacagt gctggtcaat ttttgatcat gcttttgatt
    12121 gagatcggcg gtcttggttt tatgatggtt ccgattctat gctttgcatt gataaagaag
    12181 aaagtcagtc tcagcacacg tattatatta aaagaagcgc tgaatctaga agaagtatca
    12241 ggtgtgatga agctcatgct ttacatccta aaattggcct ttgttatcca attgattggt
    12301 acggttgctt tatcttttgt atttgtgcct gaatttggct ggttgaaagg tatctggtac
    12361 agtttgttcc atgcaatctc tagtttttgt aatgcagggt ttgatttgtt aggagacagt
    12421 ttagccaatc accagcaaaa tgtttatttg atcatggtgg tatctgcttt gatcatttct
    12481 ggtgggttag gcttcatcgt atggcgtgat ttactggaat atcatcagaa aaagaagatg
    12541 acgattcatt caaaaatcgc attaaccgtt acaggtaccc ttttattagg cggattcgtt
    12601 gtgttctttt tcacagagcg caatggggca acgctagtgg aagggaatat gtttcaacga
    12661 ttggcaaaca ccttttttat gagtgtgaca cctagaacgg ctggatatta ttcagttgat
    12721 tacctccata tgagccatgc aggtctgatt ttaacgatgt ttttgatgta catcggcgga
    12781 acatctgggt cgacggccgg cggattgaaa acaactacgt taggtgtgtt gctgatccag
    12841 atgcgttcga tgttgaaagg acgtacgaga gcggaagcat ttggccgtac catccgacaa
    12901 ggcgcagtgt tgcgggcact tactttattt tttgttacgt tgacgttgtg tgtagtcgcc
    12961 atcatgattt tatcggtgac tgaaacgata cctaaaactt ctggcatcga atatattgct
    13021 tttgaggtct tctctgcgtt tggtacagta ggtcttacga tgggattgac gccagacttg
    13081 actgcaatag ggaaattgat cattatagcg cttatgtaca ttggacgagt tggtgtattg
    13141 actgtggtct tctctctgat gacaaaagca caaagaacac aatcaaaata caaatatcca
    13201 gaagaaagtg tcatgattgg ataatttcat cttctgacag aaacctagtg atttagtaaa
    13261 ataagaaaat aaaataattt ttcttgcttc ttggatactt taagtgcaaa atgttcgttt
    13321 attttttgag gcactacccg ttgttgaacc tcgggtagtg cctctttttt attgaaagga
    13381 atcaataaga tttgattcct ttatttatca ataggttata attcatattt ttttattttt
    13441 tcttttacga tacttagtcc agaagctaaa atttcaaggt tattagagga tagggaaatt
    13501 cgaacgaatg catctggttg cgcacgtgta gtaaagcgtc tggaatgata aagtcggata
    13561 ccgttagcaa gaaagtcatt ctcaattatc ttagggtcaa tctcaggatc aactggaaga
    13621 attcgatagt aagggacacg attcgttggt ttttctactt tgaaaagaga atcaaaaagc
    13681 tgattggctc tttttgtaag acttaattta gtttctaaga tcctatttgc ttcatttgaa
    13741 tggaggattt gagtgatgac ttccacatct aatccagaag ttttcacatt tatattaaaa
    13801 attgcttgtt ctatcttgct ttggacatgt actggaacta caagataagc aactcgcaat
    13861 ccagaacaaa taaattttgt cattccagct aagtagattg tttggttagg taataaattt
    13921 tggaaaggag gaatgatcgt atgttgatag taagttgtta aaaatgaatg gatatcatct
    13981 tcaataaccc atagaccttc tttttcaatc acttcggcta attgctctcg tcttttgtga
    14041 gagatttgaa accctatagg attattacaa gaaggcataa gaaagacacc atgaatcttc
    14101 ttttttctac attcctgaat caatatatca ggtcgcattc cttgctcgtc aaaatcaatt
    14161 ggaacgatct ctaaatgata taactgagcc aattcaatga aattagcgta tgtatatcgg
    14221 tctgttgcta ttctatcacc tggagagaaa agtgcagaaa gtgtgatagc caatccattt
    14281 tgaacaccgg aaacaataga tagagattct aaggacggaa tgactccttg agtttttaac
    14341 caatttgctg cagtagtcaa ttgaaaattt gtgcctttgg gatcttggta attaagcaaa
    14401 ccaacaagat ttgggttatt actagttgat tgaataaacg ggacaacgaa tttattacat
    14461 tcttcaaaag aactaaccag accaaaatca atgatatgtt cggttacttc attggtagag
    14521 atggtgatgg actcaaagac attgggagaa ataaatgtgc cacttccaat attcgtatac
    14581 agcaaacctt tctcaattcc atatttataa gccttgccaa cggtagtaaa atttaaatct
    14641 aaataatcag ctagctctct ttgggagggt aactgagtgt ttttctgtaa tttattcttt
    14701 aaaatatctt gttctaattg ttgaatcaac gatcgataat aaggacgctt caatttttgt
    14761 ttgtctggtc gccaagttaa cgggtaattg tcataagaat taataggcat tttctccact
    14821 ccttaaaatt gtataccata caattttacc attgattgta ttgattttag aagttaatct
    14881 tttgtgagga gggaattgtc tatgaaaatt attaattttg aggaagtagt agcttctttt
    14941 agttttgaag aagcgattca agtaatgaaa aaatgttttt ttgattatga aagtggtaaa
    15001 atcagccaaa atcctcgatt ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt
    15061 gcgatgatgc ctgcttattt gggggagaac agatattttg gcacaaaagt cattaccgct
    15121 tttccggata atcatcagta tcatttacct tctcatttag gagagatttt cttgtttgat
    15181 gctcaaaacg gactacccaa agcattgata aacgcaaatg cagtcacctg gattcgaaca
    15241 gctgctgtat ctgcgctggc aacagattac ttagcaaatc cgcatgcaga aacacttgct
    15301 ttaattggag ctggtcaaca agcattgtcg catttgactg cgatttctac cattcgtccc
    15361 atcactacag tgtttgttta cgatatttta gaagaaagac gaaatacttt tatcgctaaa
    15421 gctaaaaaac agtatcctaa aataacgttt ataaatgcaa tatcagtaga agatgcagta
    15481 cgtccagcgg aaatcatttg tacgttgacc tctagtaaag aggcatttct tcaaaaagag
    15541 tgggtaaggg aagacgcaca tatcaatgct gtgggcacat ttactcctga tacaagagag
    15601 ataagtacat cgttgatgga aacttctcaa atctatgtag atgattactc agctgcctta
    15661 acagaaagcg gggacctgct tatagccatg gcagaaggag atttagagga atcttgtatt
    15721 gttggtacat taggcgaatt agtttctgag aaaaaaacat tttataaagg agaatcgagg
    15781 ccgacgattt tcgacgcagt tggtctagct attgaagatc tttgttgtgc agaatatatt
    15841 tataacaaat tagagaaagg ggctttaaaa tgaacttaga ttattacgta gttgatgcat
    15901 ttgcagatga ggtgttcaaa ggaaatccag cagcggttta tgtgctagaa gaatggctgc
    15961 cagaaggtac gatgcaaaaa attgccattg aaaacaattt atctgaaaca gcatttacag
    16021 tgaagaaaaa ccaagaattt gagcttagat ggtttacgcc agatcgagaa atcgatcttt
    16081 gtggtcacgc gacattagca acagcatttg ttttatttaa ctattacaaa atacctgatg
    16141 aaacgattaa attttctact caaagcggta atctatttgt gacaagaatt aaagattatt
    16201 attacatgga ttttcctagt attatgccga aaaaagtgcc gatattgtct gaatatgaag
    16261 aagcgattgg ggctaagatt aaagaagcct acttagcacg tgatttattt ttcgtattag
    16321 aagatgaaaa aacggtggct aaattgcagc cagattttac agctatcaaa aattttgaat
    16381 taggaatagg agtcatcgtg acagcagaaa gtgtgcaaaa agactttgtt tctagaacct
    16441 tctttccaaa attaacaatc aatgaagatc cagtatgtgg atcagcacat tctaatctta
    16501 ttccttattg ggcagaaaga atgaataaaa caaaattgtc agcttatcaa atgtcatcaa
    16561 ggggcgggga tctagaatgt gaattaaaag aagatcgtgt gataatagga ggaaaagcag
    16621 ttttgttttc taaaggtacc tcgtttattt gatttctttc aaatgaatta aaaataaatt
    16681 ggtggagagt ggcaacctta tagctacaat ggttgttaaa aaaagacgca aactttttca
    16741 atttaatgaa aagtttgcgt ctcattttaa ttattggtgt ttgtttttta ttcaatcaag
    16801 agtaaaatgc cccatactaa ctatcctcgg atgacttcga ttttataacc atcagggtca
    16861 atgacaaagt agtaagaagg cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga
    16921 ccggcagctt tatgttgctc gtgtaatttt tctagatcat cagctgctat agcgatatga
    16981 ccatagccgt ctcctagatc atacgcacca tgatcatagt tgtaagtcaa ctctaattca
    17041 tagtcatccc caggaagtgt caaataaaca agtgtaaatt tgttttctgg aaaatcacgg
    17101 cggcggcttt cttcaaaacc aaatgccttt tgataaaatt cgattgatgc ttcaagatct
    17161 ttcactcgaa cacatgtgtg agccattttc atagattcat ctctcctttt ccttttactc
    17221 aatcataaca aaaagaagta aggaaatagt tgatttgtgc ttgttcatga gaaatttctc
    17281 atgtaaaatg gatagaaaat aaaactgtta aggtggaaac tattcatgga taaaccaatt
    17341 tttggtgaaa aaaagcaatt tgctgattat caaacccgtt atgcagcata tattgtcata
    17401 gaaagagatc agcagattgc agtaatcgag gctccaaatg gtgctttttt tcttcctggc
    17461 ggggaaattg aaggagaaga gacgaaagaa gaagcgattg tccgtgagct gatagaagaa
    17521 atgggaattg tggcagaaat ttcttcttat ctgggagaag ctgatgagta tttttactcg
    17581 aattatcgtg caacttatta ttataatcca ggatatttct ttgttgctga ttcttggaag
    17641 cgtataggag aaccaactga aaagaccaat aaaatttggt gggtgagccc tcaagaagcg
    17701 cttgtgaaat tgaaacgtgg tagccaccga tgggcagtcg agagatggat cgaaaaaaat
    17761 aaaaactgaa agaaagcgag tagatcagct ttctctcagt ttttttattt attacagtac
    17821 atattttttg attgcttcag ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc
    17881 tgcttctttg acaagagaaa cggcatttcc cattgctact cctagaccag catattcaat
    17941 catggataag tcgttctcgt tatctccgat agccatgatt tcatcttgat caataccaag
    18001 gtgctgtgcg agattcgcaa ctgccgcacc tttacttgct tctttattta gtacttcgaa
    18061 gtaatagggc gcacttttga ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc
    18121 aattgctgca tctaaaattt ctggttcgtc gatcatcatc attttgacga tgttcatgtc
    18181 tggtgtcatt tcttcaggtg ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg
    18241 gatcgtataa gcgctgatat ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg
    18301 gagatgactg ccgacacggc gtgccatcac ttcgatctct aggaaatctt catgtgtcag
    18361 tccgtgacgg gcgatgattt tacctgattt cgtttgttgt acgagtgccc cgttataagt
    18421 aataacgtaa tcattatctt gaaaaaggtt caactcgttt aactgttcct ggacacctgg
    18481 taagggacgg ccagtgcata aaacgatcga aacacctttt gcaacagcag cggcaatcgc
    18541 ttcttttact tcttttgtta cttctctttt ctcatttaat aaagtaccat cgatgtcgat
    18601 agcgactagt ttgatagaca taatttcctc caatatattt taatttattt caatcaatgc
    18661 gccattacgg atatggctgg caaattcttg ataagtttcg tcaaataaat cataatgatc
    18721 acgcaaggac ggatcaacca tttcttttgg aaagaaaaat cgctcatctc cttgttcttg
    18781 tcccgctaat gcagcaacaa gctgactaac ctgtgataat tctatcaaag ttccgtcatt
    18841 tcgcaaaatc tcgatttgtg tgcgatgtct gccttgttct gggcgataaa aatcataagg
    18901 tagatcgtag ctagaattca cagcagtgta ataaacagga ttatatccga ccttttctac
    18961 taacaaagtg agttcttgga ctaattttga atcccggttg ccagaaaaag tagcagattt
    19021 cagtggttta cgattcaaaa aacgtttagc taaatcattt aaaataggat cagcagattc
    19081 agaccattga gtaaagtaag tgcccaagac cccatcatca agtttgaggt attcttctaa
    19141 agtaaagctt ccgtcaagaa atggcagaag taattgagaa tggagttcaa aactgctggc
    19201 atcttcttga tacaactcgt gtgcgcgatg aagcagatgc tccaaaatta cttccatacc
    19261 acgggaaaca ggatggaagt acacttgcac atacatttga tatcggctaa cgatataatc
    19321 ttccactgca tgcatgccac tcatggaaaa agcgatcccg ccttcataag gacgaatcac
    19381 acgtaatatt cgggtaagat caaatgttcc atattctgtt ccagtgaaat aagcatcccg
    19441 aagcaaatag tccattcgat ctgcatcgat ctgactagaa atcatttgga caacttgtgg
    19501 gttggggtaa gttttttgga tcacactagc taccttttca ggaaaacctt cttctactcg
    19561 gttaagaatc tgatacactt ctgtttctgg agaagtgatg atctctacag tgatggcttc
    19621 atgattcgta tggaagatat gttcaaatgt atgagagtaa ggaccatgac cgacatcatg
    19681 caacagtgcc gcacataaag tcaccagccg ttcctgatca tcccagcctc cgttgcctat
    19741 tttttctttt gaaaaatttc gagaaaagat atcgcagatt cgtcttgaaa tctcataaac
    19801 gcctaatgaa tgagtaaaac ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc
    19861 taattgttta atgcgtcgta gacgttggac ttctttcgaa ttgatcaaat ctaagattac
    19921 ttgatgctgg acatggatat agttatggac tggatcacga aaaacttttt ccataggcag
    19981 catttgatat ttataagggg ttgtcataat gtagctcctt tatgttatta cgttgttcca
    20041 ttctctggat ttggttgttc cattgctcca gctggatttc tttttttagc cagtcggtag
    20101 aatcctgtaa atcacttgca gaatacagct tttcaaaagc ttttgcaaat gcttctttga
    20161 cttcttttac aaaaaggggt tggtcaagca gttcttcaag tgttgccatt gtagcaggat
    20221 ccactgcagg atagccattt gtaccaaagt cttcttttaa agcagattga taaaatcgct
    20281 tcatgacttg cccgcgtttt tcctgattac cgttgacact taagtacatc ataacagaaa
    20341 tcccattttt gatccgacgc tgagcgatac cggcaaattt tttgccatgg atgcttaaat
    20401 cataagttcc aggacaataa gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa
    20461 aagcgtattt agtcaatagg agcatttgct cgtaagcgtc atctatggtt attttttttg
    20521 tgtcactttg ggggaaaatc aaagaaatat tcaaaattcc ttcatctgat acgatcccta
    20581 atccacctga atttctgatc actggagcgt aacctgcatc tttcagtgtt tggatcccgt
    20641 cacttagaaa aggagttcga acatccttca tacctaagat gactgtgcag tcatattgcc
    20701 agaaatgtaa aaagatttgc tggtgttctt tagaatattc cgttaatata tctgttaatg
    20761 caaaagggga aaagtcgttt ttttgataaa tgtgctgatc tagtcgaatc gcagggttca
    20821 ttcaaccggc tcctttgtat aaattcatat tttctacaat attatactct aaaatcaaag
    20881 agttgttttg gaaaaaaggt tttctgtaaa aatggacaag ttggttgaca aaacaaaaga
    20941 aactaggaac aatgtagcta tgagaagtaa aggagaatga ataatggaat tatcacaagg
    21001 aacaccgatt tcaatcaaat tgcgtacaaa agtaaaacag caaggggaag tgcaagattt
    21061 ttattttgat ttgaaagggc aaatggtgac aattggcgat acgctgtaca tacgttataa
    21121 agagattcag gaggagacgg gcgatgagat tcctgtaacg atcaaattag ttccagatgg
    21181 tcatgtacag ctgatccgtg caggagaaat gcggatgcgt ttgaaatttg gctataaaga
    21241 acggttagaa acatcctatc gaacaccata cggtatgatc cagattgcta cttttacgaa
    21301 agaactgcat gttagtttga aagaccgtcc cacaggaggg aaggttcgaa tcgattatga
    21361 tctgttcatg ggaccggaaa aaatcggcga atattatttg accttggatt ttacagcgta
    21421 aatataaaag aaaagtttga ttttttgtgt ctgattttgt atgataagga gtagactgtg
    21481 aaaggacgtg tcctgattgg aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc
    21541 aatgatcgaa gtagcacacg cgatcttaga acaacgtggc gatgtcatgg atttttctga
    21601 tttagctaac cagatccaaa actatcttgg gaaatcagac agtgaaattc gtgattcact
    21661 cgcacaattt tatactgact tgaatattga cggcagcttt atctcattag gggataatcg
    21721 ttggggcttg cgttcatggt atgctatcga ttcgatcgat gaagaagtaa accatggaat
    21781 ggacgaagag gatgaagata ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat
    21841 caatgatgat gaagacgcga tcgattacaa tgacgatgac ccagaagata cagatctgac
    21901 agaagaagat gacgatgatc tgtttgatga cgatgatgat gaagatgaag aaatcgctgc
    21961 ttataattct gacttgcaag aaatcggagc agatgacccg acagatgatg aagaagactt
    22021 gccaggaaat atcgaagaag atttaagtat catagacgaa gacgatgatg atgaagatta
    22081 tgaagaggaa gaattttctg actctgaaaa agaataaaca ttattttctt acgagaggtt
    22141 gtgacagaga cgatcagcct atcagaataa ggaggtgtga cacgat
//
LOCUS       DARDHU010000039        21988 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000039 DARDHU010000000
VERSION     DARDHU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.039"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SDD03_001481"
     CDS             <1..395
                     /locus_tag="SDD03_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303929.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SDD03_001482"
     CDS             398..1147
                     /locus_tag="SDD03_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4303930.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SDD03_001483"
     CDS             1159..1428
                     /locus_tag="SDD03_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303931.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SDD03_001484"
     CDS             1433..1597
                     /locus_tag="SDD03_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303932.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SDD03_001485"
     CDS             1590..1892
                     /locus_tag="SDD03_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303933.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SDD03_001486"
     CDS             1889..2050
                     /locus_tag="SDD03_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4303934.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SDD03_001487"
     CDS             2047..2352
                     /locus_tag="SDD03_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303935.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SDD03_001488"
     CDS             2352..2708
                     /locus_tag="SDD03_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG4303936.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SDD03_001489"
     CDS             2695..2913
                     /locus_tag="SDD03_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303937.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SDD03_001490"
     CDS             2910..3329
                     /locus_tag="SDD03_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG4303938.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SDD03_001491"
     CDS             3326..3883
                     /locus_tag="SDD03_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG4303939.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SDD03_001492"
     CDS             3880..4176
                     /locus_tag="SDD03_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303940.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SDD03_001493"
     CDS             4253..4666
                     /locus_tag="SDD03_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG4303941.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SDD03_001494"
     CDS             5012..5386
                     /locus_tag="SDD03_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303942.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SDD03_001495"
     CDS             5423..5590
                     /locus_tag="SDD03_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303943.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SDD03_001496"
     CDS             5616..5960
                     /locus_tag="SDD03_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4303944.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SDD03_001497"
     CDS             5965..6246
                     /locus_tag="SDD03_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303945.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SDD03_001498"
     CDS             6349..6663
                     /locus_tag="SDD03_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG4303946.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SDD03_001499"
     CDS             6641..8335
                     /locus_tag="SDD03_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4303947.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SDD03_001500"
     CDS             8355..9533
                     /locus_tag="SDD03_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4303948.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SDD03_001501"
     CDS             9496..10182
                     /locus_tag="SDD03_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG4303949.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SDD03_001502"
     CDS             10182..11342
                     /locus_tag="SDD03_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4303950.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SDD03_001503"
     CDS             11352..12227
                     /locus_tag="SDD03_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303951.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SDD03_001504"
     CDS             12224..12535
                     /locus_tag="SDD03_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303952.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SDD03_001505"
     CDS             12525..12878
                     /locus_tag="SDD03_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG4303953.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SDD03_001506"
     CDS             12868..13269
                     /locus_tag="SDD03_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303954.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SDD03_001507"
     CDS             13262..13666
                     /locus_tag="SDD03_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303955.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SDD03_001508"
     CDS             13678..14286
                     /locus_tag="SDD03_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4303956.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SDD03_001509"
     CDS             14306..14668
                     /locus_tag="SDD03_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303957.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SDD03_001510"
     CDS             14671..14853
                     /locus_tag="SDD03_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303958.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SDD03_001511"
     CDS             14870..18289
                     /locus_tag="SDD03_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG4303959.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SDD03_001512"
     CDS             18340..19077
                     /locus_tag="SDD03_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4303960.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SDD03_001513"
     CDS             19087..21378
                     /locus_tag="SDD03_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4303961.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SDD03_001514"
     CDS             21402..>21988
                     /locus_tag="SDD03_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303962.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARDHU010000040        21912 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000040 DARDHU010000000
VERSION     DARDHU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.040"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SDD03_001515"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SDD03_001515"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG4303963.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SDD03_001516"
     CDS             complement(1172..1867)
                     /locus_tag="SDD03_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG4303964.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SDD03_001517"
     CDS             complement(1880..2596)
                     /locus_tag="SDD03_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG4303965.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SDD03_001518"
     CDS             2926..3534
                     /locus_tag="SDD03_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG4303966.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SDD03_001519"
     CDS             3859..4182
                     /locus_tag="SDD03_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG4303967.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SDD03_001520"
     CDS             4364..5734
                     /locus_tag="SDD03_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG4303968.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SDD03_001521"
     CDS             complement(5864..7165)
                     /locus_tag="SDD03_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG4303969.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SDD03_001522"
     CDS             complement(7519..9477)
                     /locus_tag="SDD03_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG4303970.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SDD03_001523"
     CDS             complement(9654..9920)
                     /locus_tag="SDD03_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4303971.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SDD03_001524"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SDD03_001524"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG4303972.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SDD03_001525"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SDD03_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG4303973.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SDD03_001526"
     CDS             13264..13827
                     /locus_tag="SDD03_001526"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG4303974.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SDD03_001527"
     CDS             complement(14080..14601)
                     /locus_tag="SDD03_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG4303975.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SDD03_001528"
     CDS             complement(14604..15446)
                     /locus_tag="SDD03_001528"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG4303976.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SDD03_001529"
     CDS             complement(15439..16164)
                     /locus_tag="SDD03_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG4303977.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SDD03_001530"
     CDS             16340..16636
                     /locus_tag="SDD03_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303978.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SDD03_001531"
     CDS             17204..18796
                     /locus_tag="SDD03_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG4303979.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SDD03_001532"
     CDS             18809..19549
                     /locus_tag="SDD03_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4303980.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SDD03_001533"
     CDS             19717..21066
                     /locus_tag="SDD03_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4303981.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SDD03_001534"
     CDS             complement(21194..21784)
                     /locus_tag="SDD03_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG4303982.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DARDHU010000041        21466 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000041 DARDHU010000000
VERSION     DARDHU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.041"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            53..802
                     /locus_tag="SDD03_001535"
     CDS             53..802
                     /locus_tag="SDD03_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4303983.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1226..1396)
                     /locus_tag="SDD03_001536"
     CDS             complement(1226..1396)
                     /locus_tag="SDD03_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4303984.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1590..1769)
                     /locus_tag="SDD03_001537"
     CDS             complement(1590..1769)
                     /locus_tag="SDD03_001537"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303985.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1779..1955)
                     /locus_tag="SDD03_001538"
     CDS             complement(1779..1955)
                     /locus_tag="SDD03_001538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303986.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2156..3268
                     /locus_tag="SDD03_001539"
     CDS             2156..3268
                     /locus_tag="SDD03_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4303987.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3346..3639)
                     /locus_tag="SDD03_001540"
     CDS             complement(3346..3639)
                     /locus_tag="SDD03_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG4303988.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3807..4121
                     /locus_tag="SDD03_001541"
     CDS             3807..4121
                     /locus_tag="SDD03_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG4303989.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4142..6121
                     /locus_tag="SDD03_001542"
     CDS             4142..6121
                     /locus_tag="SDD03_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4303990.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6134..6460
                     /locus_tag="SDD03_001543"
     CDS             6134..6460
                     /locus_tag="SDD03_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG4303991.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6476..6994
                     /locus_tag="SDD03_001544"
     CDS             6476..6994
                     /locus_tag="SDD03_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4303992.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6994..8346
                     /gene="celB"
                     /locus_tag="SDD03_001545"
     CDS             6994..8346
                     /gene="celB"
                     /locus_tag="SDD03_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG4303993.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8430..9935)
                     /locus_tag="SDD03_001546"
     CDS             complement(8430..9935)
                     /locus_tag="SDD03_001546"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG4303994.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10027..10617)
                     /locus_tag="SDD03_001547"
     CDS             complement(10027..10617)
                     /locus_tag="SDD03_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4303995.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10796..12442
                     /locus_tag="SDD03_001548"
     CDS             10796..12442
                     /locus_tag="SDD03_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4303996.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12491..13210
                     /locus_tag="SDD03_001549"
     CDS             12491..13210
                     /locus_tag="SDD03_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG4303997.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13689..14636
                     /locus_tag="SDD03_001550"
     CDS             13689..14636
                     /locus_tag="SDD03_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4303998.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14648..15568
                     /locus_tag="SDD03_001551"
     CDS             14648..15568
                     /locus_tag="SDD03_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4303999.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15609..17066
                     /locus_tag="SDD03_001552"
     CDS             15609..17066
                     /locus_tag="SDD03_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4304000.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17411..18025
                     /locus_tag="SDD03_001553"
     CDS             17411..18025
                     /locus_tag="SDD03_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4304001.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18018..19742
                     /locus_tag="SDD03_001554"
     CDS             18018..19742
                     /locus_tag="SDD03_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4304002.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19739..21190
                     /locus_tag="SDD03_001555"
     CDS             19739..21190
                     /locus_tag="SDD03_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4304003.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6923 a         3643 c         4338 g         6562 t
ORIGIN      
        1 tgaattagat gcgacaccga tcgagtttga gatcgaaaga agtcagacag cggtggtaga
       61 attgacgaaa gaaaaccgac ttacaccagg cggtgtcgtg ttgacaaaga tcgatgacca
      121 aagcggggaa gtcctgcaag gagctgtttt tgagttacaa gatgccaatg gaaccgttct
      181 gcagtcaggt ctaaccacag gagcagatgg aaaactggcg attgacggct tagcaccagg
      241 agtgtatcag ctggtagaaa cccaagcgcc aacggggtat gaattggatg cgacaccaat
      301 cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag aaaatcgact
      361 tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa agcggagaag tcctgcaagg
      421 agctgttttt gagttacaag atgccaatgg aaccgttctg cagtcaggtc taaccacagg
      481 agcagatgga aaactggcga tagatggctt agcaccagga gcatatcagc tggtagaaac
      541 tcaagcgcca acggggtatg aattggatgc gacaccagta actttcaaga tcgaaaaaga
      601 acaagaagtg gcgatctttt taacaaaaga gaatagaaaa gttgctgata gctcagatat
      661 aagaggaaac aatccaacaa gcagtggaaa taagcatctt ccaaaaactg gagaaaccat
      721 cattagccag tttatattgt caatattggg tgtgctactt gttttcatca gtataaaatt
      781 tagaaagaaa cgaaatatat gagctaaaat aaaaaattga ataagcatag tttttaaaat
      841 caactagtac ccatcaattg aatggaaact accgatttta gaaggggtat acaaacaaat
      901 attagtatcg gagtagagaa ggttgaaccc aaccagtaaa taagtaaaga caattttgtg
      961 ctgcgttgag aagtgaaaaa taaacctttg tagtaatact agttaataaa aaatatacaa
     1021 aaacactgcg aaaatcattc ttgagtttcg cagtgttttt tgcctaatga ctcaagttta
     1081 ttgggtaata ctttgatttt ctttctacaa ataatcagcg atcagttgta attttttctc
     1141 tagttttaat ggctctttgt caataaggag aaaaacaagt cgggtctttt gtctcggaaa
     1201 gcttcaacca actcgtgaat aaaggttacg cctccttgaa aggcgtggaa aaaccaagta
     1261 atcgatcaat caccaatttc gcatttggta gaacatgttt ggtgagctga aagtaggtgg
     1321 cgttcatgtc tgtaactaag aattctattt ctttaggatt agtgcagcta aggaaatagt
     1381 ttgttaatcg aggaattttt tgtgtaggta aattttctcg attaattttt ctgtctcgcc
     1441 atccgcggaa ataaaactca gtttatcctc aacggaaacg tgagaacgaa attcacaacc
     1501 ctcaacactc aagacagaat tcttttggaa gactttggta aatagcttct caactctttc
     1561 aatgtgcgaa taacggttgt caaagatacc taacaacttt tcgaaataaa aaataacgac
     1621 actttttcag tcagtaaaga agtgatttta tgtctgacat gatttgaaat cgagtgcctt
     1681 ggacggataa aataactttg agcgttccaa tggcttcgac agtttttaca ggtatagcgc
     1741 tgctttttta ggcgtataac cattggcata tgattatatt attcaaagtg gacaatggtt
     1801 tccttcttct tgtttttcac caccattaat ttcctatttc catctaccac agcagaacca
     1861 caatttctac agacacatgg agcagggaag agaactgcag caacgatcaa tgtttttttc
     1921 ttctgtaagg tttcgtaaga gacctctgta atcatcaaat ttttgtctat tgatctcaga
     1981 atttttttga tagaaccatt cataaagcat atcgttctct cagttgttta ttttgtggtg
     2041 atttaatcat acaagaaaac gatatgtttt tttataaaaa catgaatagg aaaaatagtt
     2101 gacaagaaaa tgacaacgcc atatgatgtt ccatagtatt tacaacctgt catagatgac
     2161 tgtaaaaaat aaaagtaaaa agaaaggacg acaacaggtg gatttttatc agtctctaca
     2221 actagatccg tttattttaa aacaaaaaat acgagaagca gcttctaaaa aagaaaagtg
     2281 catgtatatt tgttcattgt ttttacgatc attattcatc gtattgtttg ccatttgttt
     2341 tatcattata attacaacat tatttgaatc gaagcataaa ccatacgctg tggttttatt
     2401 ctgtatgttg atgagtattc gttttgttga ctttggttat aagatttccc attcgatcat
     2461 aggtttagca atcgtcatgt tctcactatt agtagcaccg tatgtccagc tgatcaaatg
     2521 gtcagttatg ggcatgttga ttcattttat tttactaagc agcatattaa tggcaaccgc
     2581 tagtgatccc aaaatgggga atgcttcttt gtacggcttt tcatatctct ttattgttta
     2641 ttcgttacct aaagattcgt tgaacaagaa tttctttaca caaacaagta gcttattatt
     2701 tttgtttttt tgttggtttt cagttctcct ttacagaaaa catcgagaaa agaacaaaga
     2761 caaaagcttg tttaaagaga tttttttgaa aggcatgtat tcgcaggaaa aaatttggat
     2821 gctcatctat gcatttggca ttagtctttt gatcgttgca ggagagtatg tcccttttca
     2881 gcgattgatg tgggcagggt ttgcattttc ttctattgta tctagctatg gattgatgtc
     2941 gattggtttc aaagaacgag cagtagaccg aatcattggc tcattgattg gatgtgtttt
     3001 atttattggt atcagccaat ttattccttt taactgggtc ggtatattag gtggtctagc
     3061 tttaggtgtt tgttctacct atagatataa aacagttttt aattcttttg gtgctttggc
     3121 aatcactgcc agtttattcg gggttccagg agcagtaacg atccgcattt ttgagaatat
     3181 ccttggggta tgtttaggaa tcatgtatat tggggtgact gaaatactta tccgaaaaat
     3241 aagagaaaaa cacggtttga atcattagat gagatcgaat aaaaaataag gaaaaagtga
     3301 agatacacat ggatgataaa ttgctatcat tccatgtgta tctttttacg gtaaatcagt
     3361 gaaacgttcg actttcattt ttaatttata ctttttacgg agttcgttta tttgagccat
     3421 catttcggat ttgtgatgct tttcgattgc ttcttcatct ttccaacggt cgatcagtaa
     3481 aagagattct ggatcttcta gtggaaagta atagtcatat ttctcgtttc cttcttctgc
     3541 acgaatcgct ttgacaatgt ttctttcgac catttcttct gcaaattttc tggcttgtcc
     3601 atttgtactt gtatacaaaa tattcactgt gatactcact aaatcccctc ctgaaaaaat
     3661 tctatcacta gtatatcacg gtgccagaaa ttattattca agatatgata cacatttttt
     3721 ccgcaatgct acggaaatat atattttgaa tagaaaacgc ttacaagata tgcttggttt
     3781 ataaaaataa aaggagtgat tgatttatga aaaaagcatt gattatttgt gcggcaggaa
     3841 tgtcatcctc gatgatggcc tcaaagacaa cggatttctt taagcaaaaa agagaagagg
     3901 tactcgtaga tgcagtttca gctacagaag gcgataatat gattaagaat agtgattttg
     3961 atttatttct gatcagcccg caaacgacga tgtttttgga taaattcaaa aaattaggag
     4021 aatctgttgg aaagcctgtt gttagtatcc ctttccaagc gtatgtccca atccctacag
     4081 gcatccagaa gttagcagag gtgatcgaag agaacatatg atggaatagg agtggatgat
     4141 aatgaaacag aaggaaaaat tgctgttgag atatttgatt gatcacaaga aggagtatgt
     4201 caccagtcaa cgtttagctt ctgaattgtt attatctgat cggacaatcc gtaattactt
     4261 gcagcggatc aaagagttag tagaaaaaaa cggaggtaag atcattgcta aaccaggtta
     4321 tggctaccaa ctgcatattt tacaaagact gacatttgac ttgtttttga gtcggcaaga
     4381 aatagtacct ggatatgcta gagaaccgca aggattttat gagtccgaag atcgacaaaa
     4441 gtatatattg aaccaattgc ttttggaaga tgccattctt tttatggatg atttggcaga
     4501 agagctttat atcagtcgtt ccagtttgac aaaggacatg caagaaatca aggagcgatt
     4561 gattccatat tcactaaaaa tcgtttctaa gcatggtcaa gggacatgga tcgaagggga
     4621 agaaagaaat cgacggcatt ttatcatgga tacttttttt ggaaaccatt atggaaattc
     4681 attgaaagaa tatttagggc aaagccgttt tttcccagac atcagttttg aagagctagt
     4741 cattatcatt ctcgatgaaa caagagaagc taagttaaaa gtttcggatt ttatcataca
     4801 gaatctgact ttacacttgg ctttggggat caaacggctc cgagaagggt ttgaaatcaa
     4861 ggaattaggc atcgaacaag aagtttctaa gcgagtagag tatcaagtgg cgcagcggat
     4921 cgttcaacgt attgaggtaa tcgcaggtgt atcgtttccc gaagaagaaa ttgcttattt
     4981 gacattacat ctcatggcta agtcgaatca caaagaaaat caaaatgatc aggaactatc
     5041 aaaggaatta gcgaaagttc tgaaaagatt gtctcaggaa tttcattttt cattagtaga
     5101 ggatctccag cttttcaatg gactactcga tcatttgtca ccgatgatga ttcgattgag
     5161 ccgaggtatt cgcattgaga acccattgac tgaggagata aagaaagaaa atcccaaagt
     5221 ctttgacgca gtaaaaaggc atttcagcaa catgccggct ttgaaaaact ataccatcaa
     5281 tgaagatgaa tgggcctatt tggctttgca tctcatggcg gcattggaaa aagaaagagc
     5341 tgcgcataaa ctacatgcat tgattatctg tgcaacgggt tacggcagtg cccaactatt
     5401 aaagaatcgc gtagtcagtg aattcggtaa aaatattaca gtggtaagtg taaaaggcta
     5461 ttatgagatc aatgaaacaa ctttaaaagg aatcgacctg attatttctt ctattgatct
     5521 ttcgacaatg tttttcaaaa ttccagtttt acatgtcagt atttttttga atgaagatga
     5581 tgttcgtaaa atacgaaagg tagttggaga atctataccc tatcattcct cagatagacc
     5641 gataccagca ttttctctac agcaaaaaaa gcagatttac accgaacagt tgtctgaaaa
     5701 atttttcaag acttatacgt acgaaccgaa aaaggaagaa attcttcatg atttgctgca
     5761 aaagctgagc ataaatgaag aagaaggata tataagagaa atgcgaaagc agatccagca
     5821 acgtgaaaat atgggacaaa tcatattcag tgatacagtg gcagtcccac atcccgctgt
     5881 tccagttgga atcgaaacaa aggtagccgt tgcattgatt ccttctggga tgcagtggga
     5941 tcaatatcaa gaaattcatt ttgttttttt agtttcgcct tcttatgtcg aaaatgaagg
     6001 aattacggtc gtgactaaag caatcgttag gttagttgac cagctaaaag tacagcagga
     6061 aatcttagct gaacctattt ttgagaattt cagcagtaaa tttatcaaac tgatcgaata
     6121 aggaggaaaa cagatggctg aaaaaataaa cagtgaaaca ctacaagtga cttcttttga
     6181 tatcattttc catagcggaa atgcacggac actgatacat gaagcatttc gctcgatgcg
     6241 taaagagatg tttgaagaag tagaagagaa gttgcgacaa gcaaatgaag agattcttca
     6301 tgcgcatcag tcacaaactc aacttctgaa agaatatgcc agcgggacaa aaatagaaat
     6361 ggaaattatc atggtccatg cacaagatca tctgatgacg acgatgacat tattggaagt
     6421 tgcacaggaa atgaattatt tgtatcaaaa aaataaatag aaaggaagga tcattgtgca
     6481 ggatacagta aaaaggaaaa tcgaattaga aaaaaagcaa cttagtttga aaaacatgta
     6541 tttcaatcgt tatctaatcg tacgttacct gactgccttt ttctttttca tgaacatgca
     6601 atggatgatt ctgcttctat cagctaaaag tttaggaagt agtttaccga tgattctgat
     6661 tttagctatt ttgcctgcag taggagaaca agtaaagctt tatcgaaaac accaaacgaa
     6721 cgttccatgg acaaagtggt attttttgtt ccagagtgtc tttaatttat tagtgattcc
     6781 agtgttgttt acctcaggat ttacattctt gtatcctttt atggcaaata acaatggagg
     6841 acagttattt gttttcattc ttgttgtaag cggtattttt gccagtgcat ttatacagta
     6901 tcgtttaaag aaaatttcac tcaaccaaga ccaacagtat atccgtatta aacaatatga
     6961 aaaagcgtta tatttaggaa aggagaataa ctaatgtttg attttttgca gaaatacctg
     7021 atgggaccta tggggaaaat tgcgcaactt aaaatcgttc gggctgtcat ggcagcgggg
     7081 atggcttcta ttcctttcac gattgtaggc tcgatgtttt tggttttaaa tattttgcca
     7141 ttaccgttcc catttttaga aggatttttt aatgcaactt ttttcaaagt tagtgacttg
     7201 tacatgatcg tcaatacaat gacgatgggg attttatcta tttactttgc cattgtgttt
     7261 gcttatgaat taacgtcaat tgaacgagat gaacaaaaat taaatgttaa tccgctcacg
     7321 ggggcattac tatctgtttt tgcattcttt atgtgtatcc ctgaattgat catgactgat
     7381 ggtaaaatca gcttgattgc ttctatcaat gagacagaaa ctgtagtaaa tggtattagg
     7441 atgggctctt ttgtcgaacg tttaggaact tcaggaatct ttacagctat tattatgtcg
     7501 attattgcgg tcgaattgta ttgtatgtgt gtcaagagaa attgggtcat aaaaatgcca
     7561 gatgttgttc cgccaggagt ttctcgttct ttcactgctc tgatcccaac atttgtcatt
     7621 gcgtttgtgg tgatgatcat caatggatta ttagttgcac taggaacgga tatcttcaaa
     7681 atgattgcta tcccatttgg gtttgttacg cagttgacga atacatggct tggtgtcatg
     7741 gtgatttatt tcttgatcca tgcattgtgg atcgtaggga tccatggagc gaatatcatc
     7801 acttctttct tgaccccgat cgtattagct aatatggcag ctaacgcaca aggcgcaaat
     7861 tatccgttgg caggagaatt caacaattct tatgtaaccg ttggtggatc aggtgcgact
     7921 ttaggattga tcatctttat tgcattcctc gcgaaatctg atcagctgaa agtgttagga
     7981 aaagcctcac ttgtaccagg attattcaat atcaatgaac caatcatttt cgggatgcca
     8041 atcgtctaca atccctattt agcggttccg tttttcttag ctccgatggc ttctgctaca
     8101 ctcgcttatt ttgccattcg gctggaaatc gtcaaaccga tgcttgcaca gatgccgtgg
     8161 ccttcaccag tcgggatcgg agcatttgtt ggtagtggtg gtgattggaa agctgctgtg
     8221 ttagcagtca tttgtgcaat catcgcattc attatctggt tcccatttat caaattctat
     8281 gataataaat tattaaatga agaaagagaa aaggcagcag aattagctag tgaaaagtta
     8341 aaatgatttc taagaaaaaa ggaactgtga caaaaaatca cagtcccttt ttttcttaga
     8401 aatgggaaac cactagtata taaataccat tattcttcaa tcaaccgctt cacgattgca
     8461 taatcccctt cataaggttc atctacacca ttgatttttt gatagggatc cacgcctttt
     8521 cccgcaataa cgactgaatc ttgcggagcg ctttctgcta aagcgcgtcg aatcgcttca
     8581 ggacgatcca tttcataatg aacgatcaaa tctggattag taattgcttc attgatttct
     8641 tccgcgattt ttttcggatc ttcaaatgct gggtcatctg ccgtcaaaaa ggcgatgtca
     8701 gctgtttctg atagcacttt tccaaaatca tgtcgacggg agattgcttt gtttcctggg
     8761 ctgcctaata caacaattac acggccatct ggatgttcat ttttggcaaa tttcagcaat
     8821 gttttcaagc ttagataatt atgtgcatag tccacataga catgcgctcc attggattgg
     8881 atcaattgat ccattcggcc tggtacacga gcttctttta atccgtcttg tgcatcttga
     8941 ctggaggcac ctgttagtgc tgcggcaatg atcgcactgg ctgcattttc ttggttgaat
     9001 cctccagcca gcaaaatatc ataagcagta tcgcagatag ctaagcgatc ttcatgagaa
     9061 gataaagtaa aaccgtgagt acctttatct gaacggatca cttgataatt gctttcttcc
     9121 gaacgaccat aagtgatcgt tggaatctgg aatagatcgg ctgtttccct aagtaaatca
     9181 aaatagtcac tttcatgatt taaaacaact gtttttgaat tcaagatcaa ttgtcgcttg
     9241 caatagaaat agtcatcaaa tgtcggatgt tcgatcggac tgatatggtc aggcgagata
     9301 ttcaagaaga ttccgacatc taaagtcaag ccgtatacgc gttgtgtttt gtaagcttgt
     9361 gaagaaactt ccatcactag atgagtcata ccattttcaa ctgcttctga catcatacga
     9421 tataaatcta atgactcagg cgttgtcaaa tgggatttga aatatgtttt gccatctaac
     9481 gttgtattca tagtagaaag caatgctgtt tttttatttg ttgtgtgatc caagattgct
     9541 tttgtaaagt acgcagcagt tgttttacct tttgttccag tgaatccgat cactttcaat
     9601 ttattttgcg gatagtcgta aaaagccata gcaagaagcg ccattgcttt acggatatcc
     9661 gttacgatga ttcctactgc tgaattttcg tatggttgtt ctgatacata atattgaata
     9721 ccctgttcga ttgctgaatc caaatattct tctttgaaat tcattccttt acagaaaaac
     9781 aaagtagaag catcagcttt tcttgaatca taacttaact gaagaaattc agctttttct
     9841 ggtacatcaa gatgccagcc tcttgttgaa acaaattctt ttaatagatt ttcttttaat
     9901 agtaaatgac ggatgtcttc taaagagaag ctcattgatt gatccctcgc tttattcaat
     9961 ttcttggttt attatagcac actcttttta atatatcgtt tgtttccgct atgacaaaat
    10021 ccagctttat tcaaattttc ttgacacagg ggacaagcgg atgagtattt tcataaacaa
    10081 tgaactgact gtaatagata ggagaacgaa tagtgtctcc aacaatccaa aaacataata
    10141 attcaaaaag acgataagac tattggtaag taaaagtcct tgcggcattg gaagccgaaa
    10201 aaatttgtgg aaaataatcg gcggtatcgt tataccgcca tttgaggccc cgactgtgta
    10261 caatgtgtaa accccaagac tgaacaaaag acttcctgaa atcagtgaaa agaaatgtga
    10321 ggagatcatt tccacttttg gaacaatctc taaaacaaca ggaaaaagca gacttcccgc
    10381 tacagtctta aaaaatactg attttcctaa taggacagcg gctagaagca gcatcataag
    10441 attgatgctc caaacagtaa ctgagcgttg gatagaaaag gccgtttcaa ctaaatgtcc
    10501 gatggcgccc actccggcgg aagctatatc gtgagggagc aagaaccaat tcaatccaac
    10561 tgtgataaag aaaagaccta aaaatataac gggtagtttt tttatttctt gtgtcattga
    10621 tatctcctat aaatgttagc cataaaaggc tgtatatttc tttactttac caaaaaaacg
    10681 aaaccgaatt caaaaaaaca attgttctta taaaagttat ataaaatcga ttgccagttt
    10741 ctgttttacc aattaaaatg atagtaatgt tttgtagaaa gttggaatct gaccgatgga
    10801 taatcaaaga aaaccagcat tagatcaatt aacaccgcaa gaaaaaatgg cacgaggctc
    10861 tgcttggatg accgccagta atatcatttc aagattgcta ggcgctgtct atatcattcc
    10921 ctggtatgct tggatgggcg aaaatgctaa ggctgccaat ggattattca atatgggata
    10981 caatatatat gcattgtttc tactgatctc tacagcagga atacctgctg cgattgcaaa
    11041 acaaactgca cgctataatt cattgaatga atatggcact tcacgacgat tatttatccg
    11101 tgcattacag atgatgggcg ggttaggcct gttgtttgct ttgttcatgt acatcgcctc
    11161 tccttggcta gctcatgctt ctggtggcgg agaagaactg attccaacga tgcgttcttt
    11221 gagtatcgct gtattggttt ttccttgtat gagtgtgata cgagggtatt tgcaaggcaa
    11281 tcaagaaatg atgccttatg cgctgtcgca gattgtagaa caggtggcac gtatttttta
    11341 tatgctattg gctacattca tcatcatgaa agtcatggat ggtgactatg tgacggcggt
    11401 cacgcaatca actttcgcgg catttatcgg tatgctagca agtattcttg tccttttgta
    11461 ttttttgaag aaacatcaag cgtatacatc ggcgtttatt cactacagcg aaaacaaaat
    11521 aaatattgca acgaaggaat tgttgttaga taccgttaaa gaagcgattc cgtttattat
    11581 tgtgggttca ggggtaacga tcttcaaatt agtcgatcag ttcactttta tgaagatcat
    11641 gtctgcatcc accgagtatt ccaatgcaca gttactggat cttttttcga tttttagtgc
    11701 caatccagat aagttgacga tggtagttat tgcattggca acatcgatcg cgtctactgg
    11761 gttaccgttg attacagaag ctgttacgtt gaaagatcga gtaggtctgg caaaattgac
    11821 aagcggaaat ttacagttgt tttcattttt tatgtttccg gcgacatttg gtgttatgtt
    11881 acttgcctat ccgctaaata cattatttta tacaccggat tctctaggaa gtaatgtact
    11941 gatccaagca agttttgtag gactcttcct aggactatat atgctagttt ccaacatgct
    12001 gcaaggaatg tttgaaaaca aggcggcaat taattatttg gttattgggt ttctagtgaa
    12061 actccttctt caatacccag ccattcgttt atttgaagta tacggccctt tattggcgac
    12121 aatgatcgga tttgctgtgt cttgtacctt gatcttaaag agaatccatg aagtagcccg
    12181 ctttaaacca agttttgtct ggcgcaggac attgctgatc tttattctta ccttgattat
    12241 gcttttggct tcgtggatca caaagatgat ttttggtgct tttttaaacg aagatagcaa
    12301 attccagtct ctattgttga ttcttgtagt agcaggtata ggggctttag tgtatggtta
    12361 tttagctttg aaaatccgct tggcagaaag actactagga ccaggaatag cgcgtttacg
    12421 acataaatta aaaataaaat aagtgaatca gaagagctag attgatgctc ttttgaaatg
    12481 ggggaaacgc atgcgtttag ataagttttt agcagatatg ggacttggca gccgcaaaga
    12541 agtcaaagga ttaatcaaaa aaggaacgat cgaagtcaat ggacaagtgg tgaaaagcga
    12601 taaagcgcaa gtaaatgaat tcgaagatgc ggttacttat ttaggagaaa tcattacgta
    12661 tcaaaaagat ttttactaca ttttgcataa acctgcagga gtcatttctg ctactcagga
    12721 taatcatgat caaaccgtaa tcgatttatt ggaagaccag gattatcggg aagatctttt
    12781 tccagtcggc cgattagata aagacacaga gggcctcttg attttaacga atgacggggt
    12841 gtttgcccat cagttactat cacctaaaaa acatgtggag aaagaatatc tagctgaggt
    12901 tagcggtata atgacagaag aagatatccg tttatttgca gaaggactga ttattgatgg
    12961 cgatgagcag acattgccgg caaaactcca aattgatcaa gtagatcaag agacagaaac
    13021 ttcaattatc cgtctgattt tgcatgaagg aaagtttcac caagttaagc gaatggtcaa
    13081 agcagtagga aaagaagtct tatatttaaa aagaatccgt atgggaaaat tttggctgcc
    13141 taaagatttg aataaagggc aatatcgacc catgaccaaa gaagagctag aacaagtgag
    13201 agaaaaataa ttgaatggat agctaaaaga ataagcagag tagttgtagc gccatctgaa
    13261 aagcagactt gtccgtctga ctttcggatg gcttttttgt gaaatttcgg cttatttccg
    13321 tagaagctac ttctggaaca ccgtttgttc ggaataaggg actgtgacaa gacttttgtt
    13381 acagtcccca ttgttccttg tgactcagag ttagatagta aggacacgat ctccctaata
    13441 ctcaggccaa aacgctgaat tcacaacctc tgataaacgg tgttcaatca gctgaccctc
    13501 ggtattagct caaaaaacgg caaaatatga ggaactattt tcccgttttt ttctctaata
    13561 ctcaggccaa aacgctgatt tcacaacctc tcctatactt tgtttcattt tccctatact
    13621 ttgtttattt attctcatta ttgaaagcgc tataattacg ttgcgaatga atcgaaaggg
    13681 gtttttagat gaaaaaacga aaggggttca atcatttttt tatgaatcta tggcgttata
    13741 aagcacttgt cctgatggct gtaccgggta tgctttggat gatttttttc ttttacatac
    13801 ctgtaatggc gaatgtcgta gcattcaaaa attttcatat ttcagcagat gggttcttgg
    13861 ctagcttgag ggaaagtcct tgggtgggtt gggaaaattt caaattttta ttttcttcgg
    13921 atcaagcatt tctcatcact aaaaatacaa ttttatataa tgtagcattt atccttttga
    13981 atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat aaacgactcg
    14041 ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc attatcagtt
    14101 actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg atcactggac
    14161 atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt atcttggtgt
    14221 tcattggtac atggaaaggg attggttata acagcattat ttactttgca tctgttatgg
    14281 gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa tggcagcaga
    14341 tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg attttagccg
    14401 taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg aattccggtg
    14461 cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg acatccacag
    14521 gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc tttgtcttgt
    14581 tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta ttctaaggag
    14641 gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca ataagacggc
    14701 taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt tgccatttat
    14761 atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg ggtacagttt
    14821 ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga tgcaggataa
    14881 aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac tgatcaatag
    14941 cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat tccgtcgttt
    15001 ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg ccaattatct
    15061 tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct tgccaatggc
    15121 tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg tctctgattc
    15181 gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg ttcagatcgt
    15241 tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat tgggttactg
    15301 gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc cactacagta
    15361 tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag gagcaggaag
    15421 ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg cgatcgtagt
    15481 catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt ttgtcaaagg
    15541 tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata aaattaggag
    15601 gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg ttgttgttgg
    15661 gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct agcaattcgg
    15721 acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa ttgatggaag
    15781 tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat tacattggct
    15841 ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat tatgatatcg
    15901 cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat ttgactgaat
    15961 tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat attaaaggaa
    16021 atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt gctcaacaag
    16081 tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat ggaatccaat
    16141 cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca aacacggcag
    16201 cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct ttagggaata
    16261 cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac caatacgata
    16321 atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa ggactcattc
    16381 caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact tggctaatgc
    16441 gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat gcagcaaaac
    16501 aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa gcacaaatgg
    16561 caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa cttctagggc
    16621 tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag ggagaagcat
    16681 gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat cagccaaata
    16741 cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa gagtctatta
    16801 cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact tcaccgattt
    16861 taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt tctaatgtca
    16921 tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa acattaccga
    16981 aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa atgcaaaaac
    17041 aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg tgacaaaaga
    17101 cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct cggtattaag
    17161 ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac tactcgaaac
    17221 taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc ctcggtattc
    17281 gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta ataatcaggt
    17341 caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact taatgtttgg
    17401 aggagaagat atgttaggga aggttttgga agagttattt atccggatct gggtcattat
    17461 aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct ttggattagg
    17521 tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt ataaagaaat
    17581 cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca atctgctatt
    17641 tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat ctgttcaaat
    17701 caaagggctc atttttttag ctattgattt tattttactt tttgccttgc tctgcctatt
    17761 tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg ttcctaatct
    17821 gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa aaataattat
    17881 tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc tgtttggtgt
    17941 cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg aagaattaga
    18001 taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga accttactaa
    18061 atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact gctaccgtta
    18121 tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg acaagtgatg
    18181 ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg atcatcaaaa
    18241 accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta acaaaaccca
    18301 tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa ttagtttctt
    18361 tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg atatatatcg
    18421 tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt ggggaaataa
    18481 aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc acacaaggag
    18541 gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat atgattttag
    18601 aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg acaaatcgac
    18661 tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa gaaatgatca
    18721 tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa aaaaacaatt
    18781 ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta gataaaaaaa
    18841 gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc attattttag
    18901 acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag caagtatcta
    18961 tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg atcgacataa
    19021 caaacacgga atatgaactg agggaacttt caatcggtat caacgaaatg ttggatagca
    19081 tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac gcacatatgc
    19141 gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa tatatccgaa
    19201 tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg ttttccgctt
    19261 tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa ctggactttt
    19321 gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc tatcatttta
    19381 tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag ccattagttg
    19441 aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta agtgtcaaag
    19501 tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa gggatgactg
    19561 aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa cttcacggtt
    19621 ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc agttattata
    19681 tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc aaggagggat
    19741 gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg ggttaaaaaa
    19801 gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga atgccaaaga
    19861 aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg tgacaatgcc
    19921 agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata attttgaatt
    19981 tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc aattaggcgc
    20041 cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc tcaagaaaat
    20101 caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct atcaagaact
    20161 gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag aaataatagg
    20221 acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa ctcatgatac
    20281 tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac gaaattacgg
    20341 agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct tctgttgttt
    20401 tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag agacagattc
    20461 tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt tgcatcaatt
    20521 ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa aagatcaaac
    20581 catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag tcgctcaaaa
    20641 aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg aagatatacg
    20701 atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc tggaagatga
    20761 ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag aacttcagca
    20821 gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg acaatgtgga
    20881 aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca aagaagtgtc
    20941 agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag aaacgaaaaa
    21001 aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac tgcttttgca
    21061 cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca ccacgtattt
    21121 ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag aaaacagtgg
    21181 agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg tctagaaatc
    21241 taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc tctcgataaa
    21301 cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtggaaagcc
    21361 attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga cctctccact
    21421 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaatgg
//
LOCUS       DARDHU010000042        20484 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000042 DARDHU010000000
VERSION     DARDHU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.042"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="SDD03_001556"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="SDD03_001556"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG4304004.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="SDD03_001557"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="SDD03_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG4304005.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="SDD03_001558"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="SDD03_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG4304006.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="SDD03_001559"
     CDS             2856..3731
                     /locus_tag="SDD03_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG4304007.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="SDD03_001560"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="SDD03_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG4304008.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="SDD03_001561"
     CDS             4628..5062
                     /locus_tag="SDD03_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG4304009.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="SDD03_001562"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="SDD03_001562"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG4304010.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="SDD03_001563"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="SDD03_001563"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG4304011.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="SDD03_001564"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="SDD03_001564"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG4304012.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="SDD03_001565"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="SDD03_001565"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG4304013.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="SDD03_001566"
     CDS             13333..14259
                     /locus_tag="SDD03_001566"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG4304014.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="SDD03_001567"
     CDS             14441..15334
                     /locus_tag="SDD03_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG4304015.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="SDD03_001568"
     CDS             15468..15593
                     /locus_tag="SDD03_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304016.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="SDD03_001569"
     CDS             complement(15727..16359)
                     /locus_tag="SDD03_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304017.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="SDD03_001570"
     CDS             complement(16507..17049)
                     /locus_tag="SDD03_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG4304018.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="SDD03_001571"
     CDS             17184..17573
                     /locus_tag="SDD03_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304019.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="SDD03_001572"
     CDS             17577..18341
                     /locus_tag="SDD03_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4304020.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="SDD03_001573"
     CDS             18470..19417
                     /locus_tag="SDD03_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4304021.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="SDD03_001574"
     CDS             19547..20287
                     /locus_tag="SDD03_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304022.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDIEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7192 a         3480 c         4316 g         5496 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata tagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agtt
//
LOCUS       DARDHU010000043        19987 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000043 DARDHU010000000
VERSION     DARDHU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.043"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..152
                     /locus_tag="SDD03_001575"
     CDS             <1..152
                     /locus_tag="SDD03_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304023.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="SDD03_001576"
     CDS             complement(230..763)
                     /locus_tag="SDD03_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG4304024.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SDD03_001577"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SDD03_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG4304025.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SDD03_001578"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SDD03_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG4304026.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="SDD03_001579"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="SDD03_001579"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG4304027.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="SDD03_001580"
     CDS             complement(4488..5066)
                     /locus_tag="SDD03_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG4304028.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="SDD03_001581"
     CDS             5388..5858
                     /locus_tag="SDD03_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG4304029.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="SDD03_001582"
     CDS             complement(5966..7192)
                     /locus_tag="SDD03_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4304030.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="SDD03_001583"
     CDS             complement(7946..8146)
                     /locus_tag="SDD03_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4304031.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="SDD03_001584"
     CDS             complement(8280..8888)
                     /locus_tag="SDD03_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG4304032.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="SDD03_001585"
     CDS             complement(8973..9443)
                     /locus_tag="SDD03_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG4304033.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="SDD03_001586"
     CDS             complement(9491..10405)
                     /locus_tag="SDD03_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4304034.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="SDD03_001587"
     CDS             10551..11354
                     /locus_tag="SDD03_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG4304035.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="SDD03_001588"
     CDS             11532..12479
                     /locus_tag="SDD03_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG4304036.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="SDD03_001589"
     CDS             complement(12547..12840)
                     /locus_tag="SDD03_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG4304037.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="SDD03_001590"
     CDS             complement(12868..13533)
                     /locus_tag="SDD03_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG4304038.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="SDD03_001591"
     CDS             complement(13673..14305)
                     /locus_tag="SDD03_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4304039.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="SDD03_001592"
     CDS             complement(14312..15379)
                     /locus_tag="SDD03_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4304040.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="SDD03_001593"
     CDS             complement(15376..16107)
                     /locus_tag="SDD03_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG4304041.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SDD03_001594"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SDD03_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG4304042.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SDD03_001595"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SDD03_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG4304043.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="SDD03_001596"
     CDS             18270..19439
                     /locus_tag="SDD03_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG4304044.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            19764..>19987
                     /locus_tag="SDD03_001597"
     CDS             19764..>19987
                     /locus_tag="SDD03_001597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304045.1"
                     /translation="MIIEINNEILEKNVYEYLIVTLFIPLSLFTVTKGPPCFSTVAFF
                     ELTVTVNPLYRNTGKTIVLPFEDLNVNVISS"
BASE COUNT         6045 a         3994 c         3188 g         6760 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaaag gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatc
//
LOCUS       DARDHU010000044        19631 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000044 DARDHU010000000
VERSION     DARDHU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.044"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            591..1280
                     /locus_tag="SDD03_001598"
     CDS             591..1280
                     /locus_tag="SDD03_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4304046.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="SDD03_001599"
     CDS             1267..2475
                     /locus_tag="SDD03_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4304047.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="SDD03_001600"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="SDD03_001600"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG4304048.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SDD03_001601"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SDD03_001601"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG4304049.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="SDD03_001602"
     CDS             complement(6578..7273)
                     /locus_tag="SDD03_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG4304050.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SDD03_001603"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SDD03_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG4304051.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="SDD03_001604"
     CDS             complement(8472..8663)
                     /locus_tag="SDD03_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG4304052.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="SDD03_001605"
     CDS             complement(8684..9574)
                     /locus_tag="SDD03_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG4304053.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="SDD03_001606"
     CDS             complement(9561..10328)
                     /locus_tag="SDD03_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4304054.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SDD03_001607"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SDD03_001607"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG4304055.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SDD03_001608"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SDD03_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG4304056.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SDD03_001609"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SDD03_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG4304057.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="SDD03_001610"
     CDS             14899..15282
                     /locus_tag="SDD03_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304058.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="SDD03_001611"
     CDS             complement(15450..16208)
                     /locus_tag="SDD03_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG4304059.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="SDD03_001612"
     CDS             complement(16226..17044)
                     /locus_tag="SDD03_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG4304060.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SDD03_001613"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SDD03_001613"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG4304061.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SDD03_001614"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SDD03_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG4304062.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="SDD03_001615"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="SDD03_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG4304063.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         5558 a         4027 c         3608 g         6438 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa t
//
LOCUS       DARDHU010000045        19480 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000045 DARDHU010000000
VERSION     DARDHU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.045"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..3660
                     /locus_tag="SDD03_001616"
     CDS             133..3660
                     /locus_tag="SDD03_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG4304064.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="SDD03_001617"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="SDD03_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG4304065.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="SDD03_001618"
     CDS             7448..7789
                     /locus_tag="SDD03_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4304066.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="SDD03_001619"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="SDD03_001619"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG4304067.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="SDD03_001620"
     CDS             9146..11566
                     /locus_tag="SDD03_001620"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG4304068.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SDD03_001621"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SDD03_001621"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG4304069.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="SDD03_001622"
     CDS             13000..13737
                     /locus_tag="SDD03_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4304070.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="SDD03_001623"
     CDS             13751..14566
                     /locus_tag="SDD03_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG4304071.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="SDD03_001624"
     CDS             14579..15226
                     /locus_tag="SDD03_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4304072.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="SDD03_001625"
     CDS             15242..15898
                     /locus_tag="SDD03_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4304073.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="SDD03_001626"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="SDD03_001626"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG4304074.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="SDD03_001627"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="SDD03_001627"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG4304075.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="SDD03_001628"
     CDS             18240..18758
                     /locus_tag="SDD03_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4304076.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="SDD03_001629"
     CDS             18865..19365
                     /locus_tag="SDD03_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4304077.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4182 g         5402 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DARDHU010000046        19126 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000046 DARDHU010000000
VERSION     DARDHU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.046"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..703)
                     /locus_tag="SDD03_001630"
     CDS             complement(<1..703)
                     /locus_tag="SDD03_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4304078.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="SDD03_001631"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="SDD03_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="SDD03_001632"
     CDS             1758..2837
                     /locus_tag="SDD03_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4304079.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="SDD03_001633"
     CDS             2869..3462
                     /locus_tag="SDD03_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4304080.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="SDD03_001634"
     CDS             complement(3572..3799)
                     /locus_tag="SDD03_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4304081.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="SDD03_001635"
     CDS             complement(3902..6490)
                     /locus_tag="SDD03_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG4304082.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="SDD03_001636"
     CDS             complement(6510..7169)
                     /locus_tag="SDD03_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4304083.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SDD03_001637"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SDD03_001637"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG4304084.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="SDD03_001638"
     CDS             complement(7942..8808)
                     /locus_tag="SDD03_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4304085.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="SDD03_001639"
     CDS             8917..9558
                     /locus_tag="SDD03_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG4304086.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SDD03_001640"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SDD03_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG4304087.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="SDD03_001641"
     CDS             complement(10999..11898)
                     /locus_tag="SDD03_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4304088.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="SDD03_001642"
     CDS             complement(11900..12697)
                     /locus_tag="SDD03_001642"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG4304089.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="SDD03_001643"
     CDS             complement(12675..13379)
                     /locus_tag="SDD03_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG4304090.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="SDD03_001644"
     CDS             13510..14085
                     /locus_tag="SDD03_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG4304091.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="SDD03_001645"
     CDS             14203..14880
                     /locus_tag="SDD03_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG4304092.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SDD03_001646"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SDD03_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG4304093.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="SDD03_001647"
     CDS             complement(16943..17941)
                     /locus_tag="SDD03_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG4304094.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="SDD03_001648"
     CDS             complement(18167..18820)
                     /locus_tag="SDD03_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG4304095.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DARDHU010000047        19058 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000047 DARDHU010000000
VERSION     DARDHU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.047"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            290..2761
                     /locus_tag="SDD03_001649"
     CDS             290..2761
                     /locus_tag="SDD03_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4304096.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2864..4600)
                     /locus_tag="SDD03_001650"
     CDS             complement(2864..4600)
                     /locus_tag="SDD03_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG4304097.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4597..5301)
                     /locus_tag="SDD03_001651"
     CDS             complement(4597..5301)
                     /locus_tag="SDD03_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4304098.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5432..5935
                     /locus_tag="SDD03_001652"
     CDS             5432..5935
                     /locus_tag="SDD03_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG4304099.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5895..6233
                     /locus_tag="SDD03_001653"
     CDS             5895..6233
                     /locus_tag="SDD03_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG4304100.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6254..7672
                     /gene="ffh"
                     /locus_tag="SDD03_001654"
     CDS             6254..7672
                     /gene="ffh"
                     /locus_tag="SDD03_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG4304101.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7784..8362
                     /locus_tag="SDD03_001655"
     CDS             7784..8362
                     /locus_tag="SDD03_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG4304102.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8366..8887
                     /locus_tag="SDD03_001656"
     CDS             8366..8887
                     /locus_tag="SDD03_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4304103.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8966..9520)
                     /locus_tag="SDD03_001657"
     CDS             complement(8966..9520)
                     /locus_tag="SDD03_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4304104.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9773..10048
                     /gene="rpsP"
                     /locus_tag="SDD03_001658"
     CDS             9773..10048
                     /gene="rpsP"
                     /locus_tag="SDD03_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG4304105.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10060..10311
                     /locus_tag="SDD03_001659"
     CDS             10060..10311
                     /locus_tag="SDD03_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG4304106.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10436..11227
                     /locus_tag="SDD03_001660"
     CDS             10436..11227
                     /locus_tag="SDD03_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304107.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11397..11918
                     /gene="rimM"
                     /locus_tag="SDD03_001661"
     CDS             11397..11918
                     /gene="rimM"
                     /locus_tag="SDD03_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG4304108.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11908..12669
                     /gene="trmD"
                     /locus_tag="SDD03_001662"
     CDS             11908..12669
                     /gene="trmD"
                     /locus_tag="SDD03_001662"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG4304109.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12805..13152
                     /gene="rplS"
                     /locus_tag="SDD03_001663"
     CDS             12805..13152
                     /gene="rplS"
                     /locus_tag="SDD03_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG4304110.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13284..14420)
                     /locus_tag="SDD03_001664"
     CDS             complement(13284..14420)
                     /locus_tag="SDD03_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4304111.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14536..15450)
                     /locus_tag="SDD03_001665"
     CDS             complement(14536..15450)
                     /locus_tag="SDD03_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG4304112.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15541..15969)
                     /locus_tag="SDD03_001666"
     CDS             complement(15541..15969)
                     /locus_tag="SDD03_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG4304113.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15974..16348)
                     /locus_tag="SDD03_001667"
     CDS             complement(15974..16348)
                     /locus_tag="SDD03_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4304114.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16634..16819
                     /locus_tag="SDD03_001668"
     CDS             16634..16819
                     /locus_tag="SDD03_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4304115.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16831..17079
                     /locus_tag="SDD03_001669"
     CDS             16831..17079
                     /locus_tag="SDD03_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304116.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17110..17289
                     /locus_tag="SDD03_001670"
     CDS             17110..17289
                     /locus_tag="SDD03_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304117.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17332..17802)
                     /locus_tag="SDD03_001671"
     CDS             complement(17332..17802)
                     /locus_tag="SDD03_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304118.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17889..18587
                     /locus_tag="SDD03_001672"
     CDS             17889..18587
                     /locus_tag="SDD03_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG4304119.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18765..>19058
                     /locus_tag="SDD03_001673"
     CDS             18765..>19058
                     /locus_tag="SDD03_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304120.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYG"
BASE COUNT         6262 a         3338 c         3747 g         5711 t
ORIGIN      
        1 ctagtatgat tatccattga gagactcctt tggtaggatg acgttttggt cgactaaatt
       61 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc
      121 cttcgatttt ccaccaacac aaaatattat agaacccttt ttttttgcta tatgacagga
      181 atgaaaggtg tttgcaagct taatgtgagg aacactttcc atatctgttc tatactggat
      241 aggagaagtt tggaaatatc cagaaaacga aatcaatcga ggtagccaca tgaagaataa
      301 atggataaat cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata aagtcatccg
      361 tctttttcca tcgtataaag aaggattaac ggcagatcaa gtcaaggaac gaatagaaaa
      421 aggtgcagca aataattcag tcgatcccac attcaaaaca aatcagcaaa tcgtattgga
      481 aaacatattt acgtatttca atttgatctt tcttatctta gccattttgc tttgtttagt
      541 agaatcctat aagaacttga cttttttacc agttatcata gccaatacag gaatcgccat
      601 ttttcaggaa attcgatcca aaaagatctt ggacgaacta aatgttttaa atacagcaaa
      661 agtcaatgtc atacgagacg ggatagaaca aacaatagat atagaagacc ttgtaataga
      721 tgacattgtt attttagaga ctggatatca aattccagca gatgcagagg tcgtggaagg
      781 gaaactgcaa gttaatgagg ctttattgac aggcgaagca gatgaaataa ccaaagaaat
      841 aggtgatcca ttaatgtccg gcagtttcat tgtttccgga aaagcttacg cccgattaga
      901 caaagttgga gcagattcgt atatttccca gctgacagtc aaagccaaga caatgggaga
      961 aggagaacag tcggagatga ttgcttctct taatcaattg atcaaatgga ttgggatcat
     1021 cattattcct atcggtatcc tgcttttttc tcaaagttac ttttttaatg gaaacacgat
     1081 caaagaaagt gtcgttgcaa tggaagcagc attgatcggt atgatacctg aaggactgta
     1141 cttgctgacg acgattgcat tagcattaag tgcgactaaa ctagcaaagc aaagagttct
     1201 tttacacaat atgaaaagca ttgagacatt ggcaagggta aatgtccttt gtgtagataa
     1261 aacaggaacg atcacagaaa ataaaatgtc tgttcaaaaa atgatcgtct ctaaaaaaca
     1321 agaaataatc agtcagtcag atcaattgga agcatgcatt gctgactatg caaaagcgat
     1381 ggcaggagac aatgcaacaa tggaagctgt taaagcatat tttacaaaaa ccactgatca
     1441 atcctatcac catgttattc ctttttcttc tgtccaaaaa ttcagcagtg tagcgttggc
     1501 agacaaggtg tatgttttag gagcgcccga aatggttttg cgcgatcaat tgccggaata
     1561 tgcggaagag tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct ttggtgtgta
     1621 cgatgggatt cttgaagaac cagtattgac agaagcagtc acaccattag gctatattct
     1681 gattgctaat cctatcagaa aagaagcaag agccacattt gattacttca aaaaacagca
     1741 agtagccatc aaagtgattt ctggagataa tccgctcacc gtatccaatg tagctgtcca
     1801 agcagggatt tcacatgctg atcagtacgt tgatgcatct aaattatcag aagaagaata
     1861 tgaagcagct gtcgaaaaat atacagtttt tggacgcgtc aagccagaac aaaagaaaat
     1921 cttcgttcaa ttgataaagc gtaacaatac agtagctatg acaggtgatg gcgtgaatga
     1981 tattctagcc atgaaagaag cagactgcag tatagcgatg gcttccggaa atgaagccgc
     2041 catgcaggcc tcacaagtgg tcttacttga ttctgacttt tcacgcatgc cagaagttgt
     2101 tgcagaagga cggcgcgttg tgaacaatat tgagcgatca gccagcttgt ttttagtgaa
     2161 gaatattttc tcctttttgc tctcactgtt ttcagtgatc tttgctctga cctatccttt
     2221 agaaccatca caaatcacat tgattagttt gtttactatc ggacttccat ctttcttgct
     2281 tgctctagaa gaaaacaaaa agcgaatcag agggaaattc atcatgaatg tgatggaaaa
     2341 agctgtgcca ggtggactaa cagatatgat cgtagtcggt gcgttggtga tctgcggggt
     2401 gactttgaat ctaaataaaa cagatgtttc tacagcatct accatgcttt tgattgcagt
     2461 agggttcctc gttctctata aaatctgttc accgctgaat aaattcagaa gtcagatcat
     2521 tcttttctgt gctagcggta actttttttc ggtcatcttt ttgcataagc tgttttcaat
     2581 caccggtatc tcagctgtat cacttttgct gttgagcatt ctatttttct cggcagattc
     2641 gatttttagg catttgacca cattggtaga atatttgttt gaacgtaaaa atgaaaaaga
     2701 gcaaaagccg tttagtcgcg tagctaaaaa atttctatcg atatttaaac gagaaaattg
     2761 acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct ttcgaagcag
     2821 gatgagtcat ccaactttaa aacgcacgag cagttgctct tttttacatt ttcggaatgg
     2881 taacggtgaa agtcgaacct gttccaagat gactgtctac agtaatcgta ccattaagga
     2941 gttgcacgta gtcttttaca atcgctaacc ctaatccggt cccgcctgaa tgtcggcttc
     3001 gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc ctgatcctca atgtcgatac
     3061 ctataccaaa gtcttggaca gaaaacgata gatcatctgt tgcctgatag cggataataa
     3121 tctcgctgtc gctttttgaa tattggacag cattttcaat tagatttttg ataattggat
     3181 aaaggatatc tgttttagta gtaaatggtt cgttctttcc aatcaatcga atcgtcaacc
     3241 gtttcttttc gatgattggc tgataatttt gaacgatttg ctggaaataa ggttctgtat
     3301 aaattgtctg gatctcgtaa gaattctcgc catcttttga cagctggatg atttcccgaa
     3361 tcagtttatc taaccggatc gcatcttttt gcatgatctc aagaaaagaa gtcagagttt
     3421 gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa accaatcagt gaagtcacag
     3481 gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt tctaaacgtc
     3541 gcactttcgt taagtcatac gccaccccta gaatctgtcc ggtaccttct gaattagtaa
     3601 agtagcgcaa actcaagtct aatgtctgcc gaccttctgt aagggtgatc tcctgatgga
     3661 tcagcggatg ctcaggagtc acttgatgaa tcagctgaat gagttttggc tcttgaatga
     3721 cttctgtgta ccgctgttca ggttgatagc ttaccacgtt taaatgtagc tgcatctttg
     3781 gattaagcag tagtaaacga gaatcagcat catcaatcag gaatacgcca atcatcagtt
     3841 cgttcagtaa cgtatataat tgctcttctg tggtagtaca cgcccgatac atcttactca
     3901 tttgttcgct caacgcatta atcgtctgat acaattcttc ccattgatcg ggtgtttgca
     3961 tgattacttc cgcctgatta ggattttgca ccattttttt tagtacgggc agcacggttt
     4021 tcaatggctg gtttttttga tagatcagat aatagatcat tcctgcaaga acaaggaaga
     4081 aagacagaaa gaagaaaaag acccaacggc gaaaactctc agttctcggt aaaaaaccgc
     4141 ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc actttttttg actggtaatg
     4201 cgacatataa aagatcctca ttcaaagtag tacttttgcg caaagcgcta cctaggttcc
     4261 caccattcaa tacggctttg atttcgggac gggagtttcg ctgttcattc aatgtttcat
     4321 tgtttgtgtc aaataagatc tttccagtag catccattaa agtaatccgt tcgttcgact
     4381 gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt tcgacatcta
     4441 attgccggat cagcagttgt gctttttttg tcaggtagct ttcttgctgt tccacgatct
     4501 gctgttgaaa ataatgggaa atcaattgcc agccaccaaa aaacaaacta actaaaagaa
     4561 taagaaagaa gagacgttca taaatttttt tccatttcat cgtttgggtt cctgaaactt
     4621 atagccaaat cctctaatag tcaccaagta ctttgggtgt tttggatctc gctcgatttt
     4681 ttctcgcaaa tgactaacgt gtacatctac gattcggctt tgtcctgcga agtcaaaatt
     4741 ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt tcatgaaata
     4801 aacaagtaat tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta cggtcacttg
     4861 ataattggtc aaatcagcag tgatttgtcc aatcgataga tattcaggct cagcttcatc
     4921 ttttgattgc ttacgaggtt caattcgtct gaatattgct ttcatacggg ctaatacttc
     4981 gcgaggacta aatggtttgg tcaaatagtc gtcagcacct atttctaggc caatgatccg
     5041 atccacttgg tcatctttag ccgtcagcat caaaatcggt gtttcatttt tttcttgacg
     5101 cagccgctga gtgatctcca tgccatccat cgttggaagc atcacatcta aaatgatgaa
     5161 atcaaatgat tgttctaatg ctaattctaa tccttcgcct ccatctgtgg cactggttac
     5221 cttgtagcct tctttttcca aattgaacgt caagagcgtt acaatagaag gttcatcatc
     5281 aacaactagt atttttttca ttcaaatctc ctttaaatag gtttcattct gtttatattt
     5341 tagcatagaa tcagtacaaa gggctatttt acaaaagaaa taaatggtat aataggaagg
     5401 cactttgctg aatagaaagg aagagcagtc aatgattgga atcagtgcat gtttaggcgg
     5461 tctggcctgt cgctatgatg gacagaaaaa agaagtacca gaattgatgg aactagttga
     5521 aactggagaa gctgtcatgg tctgtccaga agtgatcggc ggattaccaa ttcctagaga
     5581 tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg aaagatcaag caaaagtatg
     5641 gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca aagcctatca
     5701 aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc cttcatgcgg
     5761 aacccattcg atttatgacg gaagtttttc cggaataaaa aaaaatggtg ccggagtggc
     5821 tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat ggctgaaaac
     5881 tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc tttgaatttt
     5941 attcgacctt attgacagaa aaacaaatga actacatgga attatattat gcagatgatt
     6001 tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta tatgacaata
     6061 tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa actccatctt ttttctaatt
     6121 atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat aagtacccgg
     6181 aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa taaataaaag
     6241 gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg cgatgggaaa
     6301 aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc gggaaatccg
     6361 ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta ctaagcgcgt
     6421 acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac aacaaatcgt
     6481 aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag agttaaataa
     6541 atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag gtaaaacgac
     6601 ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc cgctcctaat
     6661 cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag gtcaacagct
     6721 agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg tccgtcaagg
     6781 gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg caggacgtct
     6841 gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga cgcaaccaaa
     6901 tgagattttg cttgttgtcg atgccatgac tggtcaagat gccgtcaacg tagctgatag
     6961 cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg gggatactcg
     7021 aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat tcatcggttc
     7081 tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta gccgtatcct
     7141 tggtatgggg gatatgctga cattgattga aaaagcgcag caagactacg acgagaaaaa
     7201 agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg actttattga
     7261 acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa tgatcccagg
     7321 aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg ttgaacgcaa
     7381 aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc tgttgaaccc
     7441 aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag tgaatcgaat
     7501 gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag gggatatgaa
     7561 catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag gcaaaatggc
     7621 gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat aagatgaaaa
     7681 aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc agcaattttt
     7741 attctttact aatacaatta ctctaaacga agaggtggca cagatgagat gtccgtggag
     7801 tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc ctgaatatga
     7861 tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat tgagctggca
     7921 gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg atatcaaaaa
     7981 agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag gaattattcg
     8041 taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga aaattcaaga
     8101 agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa tcgttgattc
     8161 ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg aattagcaaa
     8221 agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg cttttttaga
     8281 agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag aggtcgtacg
     8341 tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg ctataagttt
     8401 agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca ctccaacagg
     8461 taagcaaact acttatgaag ctttttatga aacaatcgat acgaccatca tgggcagaaa
     8521 aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag agaaaaagaa
     8581 tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg taaacgaaga
     8641 tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct gggttgtggg
     8701 tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat ggtatatcca
     8761 gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa catatgaagc
     8821 gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc attatgtacg
     8881 aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct gtacatagtt
     8941 gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa tctagatacg
     9001 catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca ttttcatcaa
     9061 ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc acattcaaaa
     9121 agacaagtga ttggcgtaga tggtggtttg cgccaaagcc gccgatccca cctggtgatg
     9181 cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt cttgagccta
     9241 catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga gtaacgaaaa
     9301 cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca ggagcctgtt
     9361 ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca aatacacttt
     9421 cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg taagaatctt
     9481 ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc ctccttcaaa
     9541 ctatctataa taatcttaca aaagataagt aactggagca aataaaaaca cttactgaca
     9601 aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa aatcttctat
     9661 tttctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac agggtaaaaa
     9721 aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa taatggcagt
     9781 taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta tcgtcgtagc
     9841 tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt acaatccttt
     9901 gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt tatcaaaagg
     9961 tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta tgcaaaaaca
    10021 ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt aagcgactta
    10081 gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt agacgtcgta
    10141 gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat tggccgtatc
    10201 attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg tgttcggatc
    10261 aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta aataaacgtg
    10321 ctgtactcga attttcgagt gcagcttttt tgtttttttt attaattgaa gaaatgatta
    10381 tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac agccaatgag
    10441 aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt cggttactca
    10501 agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca gtccagaaaa
    10561 aagtagtacc aaaacggctt ccaccactga acaaacaatg gctacctcaa ataatgagaa
    10621 aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc ctctagatgt
    10681 accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag gttattctat
    10741 tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga atcaagaaac
    10801 gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa caatccaagt
    10861 tttgcagcca tttgagatcg acacaaatgg tcagcaagtg gacttaggtt ctcgaatcac
    10921 agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag gaaactggcg
    10981 tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag ctaaagaaat
    11041 cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta aaattactgt
    11101 tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac cgaaaaatgc
    11161 ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt ctatgaagag
    11221 gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac aggaagtaat
    11281 caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa gaaaactaga
    11341 attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga tataagttga
    11401 ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc gaagtccgtg
    11461 ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg ctaacactat
    11521 tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa cataaaaatt
    11581 ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa tatcgggatg
    11641 gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa ttctattacc
    11701 atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa gtaaaagaaa
    11761 tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa gctgatatat
    11821 tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga gtggtccgtg
    11881 tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt atttccaaga
    11941 atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa aggattatta
    12001 gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac agtagatgat
    12061 tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta tgataacatc
    12121 caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct tgacccagct
    12181 ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca cttagtattc
    12241 atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac agatgaagtt
    12301 tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat gatcgatgca
    12361 acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac ggattcttat
    12421 tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa agggatgaaa
    12481 gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca attaaaagaa
    12541 tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga atggacacct
    12601 caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac agcagatgaa
    12661 gccaagtgac tttttctaaa atcttcttta cagtaggttg ataatatgtt agtattttat
    12721 ggtgagtgca aaagcactta actattacga tattccgctg tgagaaaagc tcataagaat
    12781 atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac aaaagaacaa
    12841 ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca cgcaaaagtt
    12901 gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa acgccgcggt
    12961 gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg cgttgaacgt
    13021 acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta cggtaaagta
    13081 cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg tatcaaagaa
    13141 atccgtcgtt aatcatcgtt atcagactga gaaagaaccc ttggtattca agggttcttt
    13201 ttttgttctc tgttttttta tattaacgat ttctatttgg gacatttttg attaataaaa
    13261 aggggcaaaa aaggggcaac aagttacagt ttttctatat cttctttcaa tttttctttt
    13321 actccctttg tcacatgcaa ataaatcttt tcagtgatat cactattttc gtgcccaacg
    13381 cgatcctgaa tagcatacag aggagttcct agttccgcta attttgaaat atgggtatgt
    13441 ctaaaaatat gtgaactaag ttttttatca atcttcatgt cagctttatg atttctcaaa
    13501 taggtgttaa ttgctgttag ttggaaagga gttcctttcg ttgtttgaaa tagaaattga
    13561 ccattcggat ttaattctag aagctcattg taaatagcta tcgctttttt tggtaaatca
    13621 atttctcgca ttccagcagc agtttttgta gaatcagatt ttttcatatc agctattgaa
    13681 cgttctctat acatcatcgt cccatttata actactgatg cattattatt ggtaatgtgt
    13741 acatcgtctt tacttaatgc gattgcttct ccaggcctca ttccagtcaa atatagccac
    13801 tggaaaagga gagaatatct tttattgtgt gaagttgtga aatttactag tcttttatat
    13861 tcatcatctt ctaaaaactt atctttaatt tttatcgttt ttgactctcg tttataatca
    13921 ataactactt cgttgatagg atttttttct acatagcctt ttttcatagc gtatgaaaaa
    13981 agaagattca atttggattt aattacactg acgtacttat ttgacaagtc atctttatat
    14041 atcatatctt caaaaatatt atttaaatct actgtattaa taccagaaac gatgtaagtc
    14101 tcaggtatct ttttctttat agtgtttaag atattattcg taggataata tgtagactcc
    14161 ttaacttgtc ttttgtaaat aactaaccat tcttcgacaa gctcatgaaa tgttagatca
    14221 ggcttctgaa gtgttttttc gtttaatttt atatcaattt ttttatttaa ctctagcaat
    14281 gccactttct gagtttctcg agatttattt ttataagtaa tgctcacttt ttttcttttt
    14341 ctagtttttg gatcaatata tcgctcatta tatttatata ctttttttcc gtttttatca
    14401 gttttggttt caatccacat tgttttcatc tcctatttgt tgctataata ggaatggata
    14461 agtaagccaa ttatagcagg tttatttttc atcacgtcca caaactttgg cgagggaggg
    14521 ggcgtgtttt tttgtttagt agctaccttt ccatgaagta tctgcgaaga acacttcaaa
    14581 tgttgttcca tcatttttca cttcaaaata tgctgttcct tgtgcggatt tacccggttg
    14641 gatggtttcc gaaaagtaat ctctactagc taaatcagat ttaatatctg atgaatcata
    14701 aaattctaaa taacttgaac ttgcatcaaa aggtgtagtc cctgtgtttt ctacaagaaa
    14761 atctaccttt gcgtaaaata ggccttctgg tttatagaaa tcatcaccgt aatcttttga
    14821 tacagactgg acagttacgt taatagagtt accattttca gaagtaaaag gaactgcttc
    14881 tcctatacta tgtgcaactg atgccgtctg gggttctgaa gacgaagttg tagttgtagt
    14941 tgtagtagag cttgaatctt tggattttcc atctttatct aagaaagttc gttgattata
    15001 atatacaacc tgttcagttt ttggactaac ccaaattaac attgtttcat catcagaatc
    15061 agttgaataa gtatattgga gcatctctaa tttttttata tcttttgcac tatttccatg
    15121 ttcaattaat tcagagtgct ctttataata atctccttgt gcatcctttg aaatagatac
    15181 aattaattca tcccgtgtga tagcattgtc taattctttg taaacaaaat catttgtcca
    15241 tttacgtaac ggtgaaccaa gtgtctgata aacttgagtt aaatcatcac caacttttat
    15301 tttatgaacg tctgatgaag ttagtttttt tgtttcatat tcagatgttg atgaaacagt
    15361 attttcttta gtgctagcag aattaggact agtggagcat ccgcttaata ccaagcctaa
    15421 aaaaagcaac ccaaaaacaa tttttttcat ttttaattcc tcacttctat gatatgattt
    15481 ttttgtagga tcttagaaat aaggttttta gtccgtgttg cagcacggac tttttttaca
    15541 ttatataaga aagcgataag cgctttctgg aagtccgtaa agattcttta attcctcaat
    15601 tcgtttagga tattgatcat tgtcttcttt gtatagagaa acaatgagat tagcagcaaa
    15661 gcaattagct tcgctttcag atttgcttct agatgttctt gttgatacat agtaactgga
    15721 taagccacga tgaaaaatag cgtgacctaa ttcgtgagcg caaatgtaga atctttcctc
    15781 agagtctctc agttcatcat ttaagaagat tattgcacga cctctaattt cttgaaactg
    15841 tcctttggga ttctcgataa aaggaacgta ttgaatttta atgcccatct tttcacaaat
    15901 ataaaaagga ttagcggact ggtatttccg cttcaactcc tcgactaaat taatcgtatc
    15961 catctccata agctcacatc tttttgcctt tttctttgtc ttctttcaca atatcccaga
    16021 aggttccaat aaggatatct tttacgcgtt ggacctgttc aggcgtcagt gtttctccgc
    16081 cataagacat attgacattt gaatctagta atttatcaag ttcaactact tcatcttttg
    16141 ttgcccagtc aggaacttgg tttctaccga gtaaataatc agtagttaca ttaaaataat
    16201 ctgctacaag ttgtagtctt ttagtactag gagttctatt tttccattga taaatcgtgt
    16261 ttttaggtat atttaattct tcttccaatt gagttatact tatgcctctt ttatgagcta
    16321 attcctttat tctatctagt aaattcattt tcgcacctca caagctacga aaacactaat
    16381 aaaaaaatta gttaaaggtg ttgacaacta ataaaagtgt tagtatactg ttttcgtaag
    16441 ctaaaatatt agctaaaaag acttgataac taataaacac ttcacggtcg gcaaactctg
    16501 aaatgtaaat tactaggcat ttctgtgtct tatttagcta tgtctatata ctaataaaaa
    16561 tattagttat tgtcaacgaa tattagctaa ttttttagct tacagattat ttttttagaa
    16621 aggagctatt tttatgtctg agaatttaga cttaaaaatt cgagcggaga tgagaaaaag
    16681 aagaatgact ttcaaagaac tagctgcgct tatcggtatt tcaggagctt atttatcaga
    16741 tattctaaac ggcaatcgtg atggaaagaa agcacaacag catatcgaaa cagtgaaaaa
    16801 aatattggac atccgatagg ggggtaggcg atgacaaaac taaaaaaaca agattttgta
    16861 aaaaaataca attattctcc atctacttat caacgtcgaa tgtcggaact aaaaaataca
    16921 gcaattttct cagcggcgta tgaacgggtc acaggacaag aagtttggat caatacagaa
    16981 ttatacgata aatttttgtc tttcaaatcc tataacaggt tacgcacaag aaaggtaaca
    17041 cctaaagaat ttatcgagaa gcatttagtt gatttataaa aaacagaaca ttttgagagg
    17101 tgaaggttaa tgggtaaatt caacagagca ttggtattca gcgcaccgct aatcatctac
    17161 gctttaggac tttggggaag caggcaagcg ttgataggaa cgattgttta catggtttgg
    17221 atttttatgg ggcttgatga agctgaagct gagtacagag cgaaaaagcc aaccgagggg
    17281 gctgactaaa aatgaataga aaagagaaac tagaatgtat attactatta ctcagtttaa
    17341 ttctttcact aatttctcta ttgggaagtt tgtatttttg atatcaacaa ttttgtggat
    17401 ttgggttcta tttagagtaa atatcatttt tgtttggtga ttaaggagtt tattaaaatt
    17461 aattttccct acttcaaatg caaggtaaca agatatagca cttttaggag gaatatttag
    17521 cggtacagct tcagagtata ttctattatc atcgactgat cctcctttag caattaattt
    17581 cttaaatgga gtagctgacc atctgcggtt taagttagcg gatgaaagtt ctaaattaac
    17641 taacatagct ggttctgttg aatagttaga aatgattact tttgtataaa tcatatctgc
    17701 agcgaagtaa gaagcattaa gttcaacttc tagttgaggg cgcattcctt ttatttttat
    17761 ccctgttaaa aacgtactca atataaaact tattattgac atccattgaa aaattgttaa
    17821 gtgaaaaaaa ttcaaaataa acaccaccag tttttaacta aattatacca aaaaggagag
    17881 aagaaataat gcaagaatta gtaattttga aaaataaaga agctgtgact acgagcttac
    17941 aagtggcaga aagcttcgag aaaaaacatc aacatgtttt aagagatatt gatgcactaa
    18001 aaaaagatgt gtccaatttt ggacagatgt ttgtagaagg taatgaacca gattcatatg
    18061 gcagaaatcg acgagttttc ttcattagta gagatggatt tttcttgctg gctatggggt
    18121 ttacaggaaa gaaagctatc tacttcaaac aaaaatacat tgaagcattc aacgaaatgg
    18181 aagatgttat tcgcaagaat actgttcctc aaacaattga agatatgatg atctatcaat
    18241 tagaagaaat gaaagatgtt aaaaaagatg tttccatgct taaagatact atgcgaatta
    18301 gcggacaaca agagtttgaa attaagcaaa aaggaaatat gaaagttatg ggagttctag
    18361 ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca aaattttggt
    18421 ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt aagagattcg
    18481 atgatgctgt ttcatttatt gaaatgtggt tgccagaaac tgcgatccgt atggaaattg
    18541 atcaactgaa cagacaacag agactttttg gtgatgacaa tgaatagagc tgaagcgcta
    18601 agaataggga cggcaattgc taattgctgg tggaaatact ataaaccaat catcctaagc
    18661 caacaacata ttgacaagca aaaagcatgg caacaaataa aaaagtgact ccgccggcaa
    18721 gcaaagagtc acaaagaaaa cacatcataa ggagatttta gcatatggaa aaagaacttt
    18781 ccactctaga tcaatatttg attgatcctg attggggcaa gccgaaaatt gaggaaacaa
    18841 gtggtcgaaa aatcagacga aatcttttga cgaatgaaga actagcttgg gatcaagatg
    18901 atttaggcaa ccatgtaact atttgggatc atgtttatct tatccatcta tcgaagcatt
    18961 cgaataaacc tgaatatatt tacgtcatcg aagatggctt gattgatgcg ctagaagagt
    19021 acgacagaga taacttgatt gatatctctt attacgga
//
LOCUS       DARDHU010000048        19032 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000048 DARDHU010000000
VERSION     DARDHU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.048"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(47..1615)
                     /locus_tag="SDD03_001674"
     CDS             complement(47..1615)
                     /locus_tag="SDD03_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG4304121.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1619..1900)
                     /locus_tag="SDD03_001675"
     CDS             complement(1619..1900)
                     /locus_tag="SDD03_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG4304122.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1897..2820)
                     /locus_tag="SDD03_001676"
     CDS             complement(1897..2820)
                     /locus_tag="SDD03_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG4304123.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2817..3695)
                     /locus_tag="SDD03_001677"
     CDS             complement(2817..3695)
                     /locus_tag="SDD03_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG4304124.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3682..5019)
                     /locus_tag="SDD03_001678"
     CDS             complement(3682..5019)
                     /locus_tag="SDD03_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG4304125.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5022..6125)
                     /locus_tag="SDD03_001679"
     CDS             complement(5022..6125)
                     /locus_tag="SDD03_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4304126.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6199..7707)
                     /locus_tag="SDD03_001680"
     CDS             complement(6199..7707)
                     /locus_tag="SDD03_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4304127.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7673..8365)
                     /locus_tag="SDD03_001681"
     CDS             complement(7673..8365)
                     /locus_tag="SDD03_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4304128.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8387..9361)
                     /locus_tag="SDD03_001682"
     CDS             complement(8387..9361)
                     /locus_tag="SDD03_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4304129.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9661..10533
                     /locus_tag="SDD03_001683"
     CDS             9661..10533
                     /locus_tag="SDD03_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4304130.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11115..13313)
                     /locus_tag="SDD03_001684"
     CDS             complement(11115..13313)
                     /locus_tag="SDD03_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4304131.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13545..14537
                     /locus_tag="SDD03_001685"
     CDS             13545..14537
                     /locus_tag="SDD03_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4304132.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14597..16018
                     /locus_tag="SDD03_001686"
     CDS             14597..16018
                     /locus_tag="SDD03_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG4304133.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16011..17510
                     /locus_tag="SDD03_001687"
     CDS             16011..17510
                     /locus_tag="SDD03_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG4304134.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17549..18943
                     /gene="uxaC"
                     /locus_tag="SDD03_001688"
     CDS             17549..18943
                     /gene="uxaC"
                     /locus_tag="SDD03_001688"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG4304135.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKKMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5715 a         3877 c         3367 g         6073 t
ORIGIN      
        1 aactagtgaa aataagttag tgaacctcat ttattaaaga attaaattac agatgatctt
       61 ttccatcata cttgaaaaac tcaaaatcag cataacttcc tcttttttta tttagatcat
      121 gcactgctat cccgataaaa tttccagtaa accctcctga gagtatacac atgttttttt
      181 gcggacctac tttcttccat tcttcagttg atacgtcttt caaatgaaac tgtgctgtct
      241 ctttctttac agtgactttc aaaaaagtct tcttttctgt gagtggaatc gtccaatctt
      301 ctatttgtgc tactccgttt tctgattcta tcatacgtag gcattttccc ttgtcctcat
      361 tccaagtaac ataacaataa atataattgg attcatttaa gtacagtacc agtccagcca
      421 tctgattaaa attattaggc atgtattcaa atgctgtctc cgcatcaaaa gtaaaatccg
      481 tttgccgaat agctaacaaa tgatgttcga aggtagattg aagagattca ccggaaatca
      541 gacgtaaaaa acctggtcta cattttgtat cacaccaatc agcatttggc aaaatgcgtc
      601 tggcattcca gttcttcttt agctctcctt caaatgtatc cataaactga tgatcttttt
      661 cttgttgtac ctcttcattt gtctcaatca ttatttcttc catcggagtt gtctggctat
      721 ctttaagtct taaccaatcg tcggacgtcc aatacacttc ttgaatagcg gtttctcttc
      781 ccaaaatagc cgcaccgttt aatggtctgg tacaaagata tgtcatgtac catcttcctg
      841 acggggtttg tactaagctc ccatgtcctg tacattgcaa tgtagaagca ggtttgtcac
      901 tagctgtcag cataggaaaa ccaggatcaa gctcgtaagg accagtaata ttctttgctc
      961 ggcaaactgt aactgaatgt cctcttcctg taccgccttc tgccgtcatc aaataataat
     1021 acccgttacg aaaatacaaa tgtggtgcct ccgttttcgc taattctgta cctgcaaata
     1081 ttttatagat gggaccatca agcttcttct tctctgcatc atattcttgt aatacgatac
     1141 cgctggattt attcggtgtg tccagacgat aatcccatag ttcattgaca agccattttt
     1201 ttcccgtttc aagatcatgg aacaaagaag gatcaaagcc gttactattt aaataaattg
     1261 gttcggacca aggacctgtg atctcagatg cagtgatcaa ataattgtgg acatctttaa
     1321 acggacggcc tgcacttttg acatctgtgt aaacaagata aaacaatcca tcagcataac
     1381 tgatctgtgg cgcccatata cttccatttg taggatttcc ttgcaaagat acttgagtgg
     1441 tcaaaatatc cgttgtatgt gaccagttga ctaaattttt cgactcatat actcggattc
     1501 ctggaagcca ttcaaaagag gagacaataa tgtaataccg ctctttcact cttaatatat
     1561 taggatctgg attgaatcct cttaaaatag gatttttcac tttgatagtc tgcatgattc
     1621 aggctccttt tctcattctt actcgatata aactgaatgc cccagctgct aaaaaagcaa
     1681 gtgaaataaa caacacaacg gcaaacagac cagtcatgtc ctgtaataat ccaaatgtat
     1741 tttctaaaga caaagttgaa tgatctacag caagcgactg ataacttttt ttaaactctg
     1801 acgcagacat attacttaaa gtaactgcta acccgccaaa gaaaatcaaa gatagactgc
     1861 caccgaccat tcctagcaat agttcccatt ttcttttcat ttctgaaccc cctgcttttc
     1921 taaatgtatc cattcaggtc ttttgatcga tgtttcataa ttgttattac gttcagagaa
     1981 tttgacagtc tgttcatttt tcttgtcatc ccaattatcc caaccagttg ggtgaacatg
     2041 cccacctatc ttacacgctt caaataagac atttgcatac ggacgccacg gtcttcctaa
     2101 atagacgttt ctagtatcag gttctgcagt caaggtacag ctttcaaaac gataccctgt
     2161 tttttgattt ttcggcgtag atgctgctgt gatgtatcca ggatttttac tggctaagct
     2221 atggatatga cactgatgaa aaacggcatc tgccccacca aatatgaaat ccaccgttcc
     2281 ttcaatccag caatattcat aggtctgacg acaatactca ggattggcag aagccggtgt
     2341 acaaaaagga gtaccatctt tttgcaaatc aggcaacgga cccgtgcata aggtatcctg
     2401 ataaccagat agtcgacagt tttttaaaga tacgcgatct cctgaattga ataaagcaac
     2461 agcctgtcct accttttctc ctggacctgc tgtattttcg atgtgaagat tttctaatac
     2521 gacattcgat ccttctaaga aaaaagtagc acttcggaaa gtccctaact ggctaccgtc
     2581 aagatactcc tgttttgcaa aacgattacc ggagataact actgttcctg ctcccacaag
     2641 tttaaaattg gataatctgc tttctatata ttcatcatat cttccttctt ttactatcaa
     2701 gacctttgga tggctatccg gcaggcctgc caaataatcg attccttctt ggatagtttc
     2761 aaaatccgtc ggggtatcta tagcattctt tccaattatc agtttgtctt tcatcctcat
     2821 aaatgctccc ttatgaattg tttccaaata tttctcattt cttgtgtttc catatgaccg
     2881 cctgttaaag aaaaagaaga aaagttttct ggactaccca ttgattgata ctttttgtcc
     2941 agcccttttc tcagcttcag tacgccttcg tccaagcaca tcgtatcgtt ttttccaact
     3001 aagctaagac gagggcgcgg agcgattttt tcttggattg ctaacgttgt ataagctgtc
     3061 aatagatttg gcacatagta ataaaaccca tgatgatcta ggcggcgatg tagcaaaagt
     3121 gtttcgatgt ccacctgtgc cgctagatca atacaaactt tgacacgaga atccaatgct
     3181 gtcacccacc agcttaataa gcctcccata gaaagaccaa tcgttgcgag ccgttgagta
     3241 tctgtgtctt cccttgtctc caaataatcg atcaagctga tcacatcata aatacgcatt
     3301 ccccataacg tttttcctgt taataaaaat tgtttgaaca actcgctttc agaaatacct
     3361 ccgcgttctt caaatcccca agcatcaatc gaccaaacag catatcccat tcctgtcagt
     3421 acttctgcaa aagatggaga agctaagtaa ggagcaccat ggatcaattc acttttcccg
     3481 actgaaaaat ctccgccgtg agaatgcagg taaaggaccg tcggaaacgg tccttctttt
     3541 tctggataag catagagggc tggtactgtt tccaattcat ttaagtacaa ttgatagtat
     3601 tcaagcataa aaccgttttg tttttctcgg tataatcgtt ttccactagg ggtatgtctg
     3661 ctagatggag cacctaaaaa atcattcaac tgcataagca aagtttttac gaacaaccag
     3721 ttcatccata ttttctgatt gatttgattg gaatttctca atactgattt ctttagtatt
     3781 ttctaataga aatgctggtc cctcgtgatt ttcaatcgac atctggttaa aggaaacctt
     3841 acccgcaaag ccaatataaa atccttgtct gctcatttcc tctattcctg tcatcatagc
     3901 aggaacaccg ggaactgcat cttgggccat cgagacatcg atattgtcaa atgtgatttc
     3961 ttgtatgaat tgttccgcta atccataaat aaaacctgca gaagcatgga cattccttgc
     4021 cgtgatattc gaaaaatgga tccttctgaa tgctgatgtc cgttcatcta tcggataagc
     4081 ctttttttcc catacatacg gttctttccc tcgcggacca cagaaataat aaagattcag
     4141 tataaatgga cacatcacat tatccatgac tatattggat actcgaatat cttcaacgat
     4201 cccgcctcga cctcgtctag atttcaatcg tattcctcga tcagtttctt ggaagataca
     4261 atttgaaatg gtaatattac gaatactacc actcatttca cttcctaata cgacaccgcc
     4321 atgtccatgg accatcgtgc aatttgtgat cgtgatattt tcgcaagcga tcctttcgta
     4381 cgtatcttca gtccctgcct tgattgcaat acagtcatct cccacgtcaa tatggcaatt
     4441 actaatacgc acatttttgc atgattctgg atcaattccg tctgtatttg gtgaatccgc
     4501 tgggttcaag atcgtcaagt tatcaaacgt tgcattactg caaagaatcg ggttgatcgt
     4561 ccaacttggt gactggatca atttaatatc tttcacagta attcgatgac agttatggaa
     4621 actcatcaat ttaggacgag gataagcgag attatctggt tcatttctaa aagtatgcca
     4681 ccattttttt ccatttccat ctaatgtacc gaaaccagtg acagagatgt tctctacatt
     4741 ttgagcatag atacaggaag cataaacttt tcgatggact ccttcccaac gtgaatgaac
     4801 aacaggataa tcttttggat cgtcagagaa tttcagaacc gctcctgcgc ttaaatgaag
     4861 ttcgacattg ctttttaaaa ataaagctcc tgttagaaat tcacctgctg gaacaactac
     4921 ggtcccgcct ccgtcacttg ctgcagcatc aatggcttgt tgtattgctt cagtattcaa
     4981 ttcatcaatt gatgcgccaa atgtcaaaat atcatacata tttataatgc ctccacttca
     5041 atcaatgctt gcaagaatgc accaacgcct ttttgatcat tacaaataat tggttcactg
     5101 atgtagtaag catatgaacc atctcgctga tctgctccgc ctaaccctgc tacttgacag
     5161 tttttattca agttcagcca gccttctttt gtttccagaa caaattcatc aagtaatcct
     5221 tgatgtgatt tcttcacttg tggaagccat tcttctttgt tcagcagtcc caaacgtatt
     5281 cctttagcca ttgcgtaaac aaacatacta ctgccggaag cttccagata atttcctttc
     5341 cgttttgttt ctgttgttac ttgataccat gtcccgctgt tttcttcttg atatggaagt
     5401 aatgcagcca gtgtcttttc tagaatttct ttcagcaaag aacaatctgt ttcttcatga
     5461 agtaattcat aggtatctgc tgcagccatc aaataccagc caatcgatcg gctccaaaaa
     5521 tgatgggata gacctgtttt tggatcagcc catggttgga ccttcttttc atcccatgca
     5581 tgaatcagca aatttgtttt tggatcgaat agatgtttat aactgatttc aaactgtctc
     5641 aaaacatctt ggtaatcttt ttcttcacta aaattctgga taaattcagc ataaaaagga
     5701 gaacccatat ataagccatc caaccaaatt tgataaggat aaatttcttt atgccaaaaa
     5761 gctccttcgg atgtgcgtgg atggtttgcc aactgctggg aaaggagttc tgctgctttt
     5821 ttatattttt cttcgcctgt ttctttgtac aataaaaaga aaagctttcc tgtatttaca
     5881 tggtcgatat tgtattcttc aacgctgtag ccacggatcg ttccatcttc ttgcacgaaa
     5941 tagtcgatat tgtcttttat gaaatcaaaa taaatttttt cttgggttcg ttcccaaagt
     6001 aattggaacc ctttaagtac gacgccatag tcataagacc attttccttt ataccacttt
     6061 tcttcatata agcgaggtgt tctttccatg atactttttg ctaatttctt tgaccagttt
     6121 tccattattt gctctccttt aaaaattaaa ataggatttg ggacaaatac caagaagtat
     6181 ttgtcccaat cgtctatatt actgtgccgc tgctttgtat gcttcctcgt actcttgtgt
     6241 gattcgatcg cctccttgtg ctttccagtc ttctacagct tttttgaatc catcttcgtc
     6301 aagctgtccc atgatgtatt gcaccgtagc atcaatgacg attttttcaa tttctgatcc
     6361 tacttcattg ttcgtcggtg aatccaatgg cacagaagga tccattacag caaattcttc
     6421 attttctttg atcaattgat tagagaattc tttttcaggg tctgtatctt tgaaagagaa
     6481 cgtcacttca ctaggacgag agcttgataa cggttgcacc tcttgttgcc aaagatccat
     6541 atcgataaat tcgatagaac catcatcatt caattcataa tgcgttcctt cgataccgcc
     6601 agtcatcaat ttatacacat cttcttctaa aagatcatca acaaattgaa gtaatttctt
     6661 caattgtgct tcgtctttta cttgagattt gggaaatgct agcaagccgc caataccatt
     6721 tccttctgac caaacatgat aaccgccgcc ttcaccatct gagatcttgt ttacaggcac
     6781 taattccata tcatcttgaa taccttgacc catcgtcttc agattcttga tatccaccat
     6841 tccagtgtag attccagttc ttccttgtgc aaattgttct tgctgatcag tcttttgggt
     6901 aactgcaaag tcttgtgcca aatagccatt ctcaaataga tctcttgaaa aattcaaagc
     6961 tttcatatac tcttctgtat caaattctgg agtgaatttg ccattatcgt ctacagccca
     7021 gccatttggt gctccaaaat aagaagtgaa aagtttaaag ctggtataac gaatatcact
     7081 aggcgcacga tcgccaaaac caactgtgtc gtccacacca tttccatcag gatcgttttc
     7141 tgtaaattgt ttagctactt cataaagctc atccatggta gttggaacat ctagtcctag
     7201 attatccaac cagtcttttc gaatgactaa gccagcacga gcgtagtctt tttgtatcgg
     7261 tacgccgtac aaggcaccgt cgatactcgc agcttcgatt ctatcttcag aaatttgact
     7321 taaattttta tagtctttta aatattctga tacatcccag aatacgcctg attttaacgc
     7381 attgcggaca gaagagtttt tcaacatcgc aatttgaagt gtaacgatat ctcctagttc
     7441 atcagaagcc aatgcagttg ttaatctttc ttcttttgaa gcatcaggaa tccagttgaa
     7501 tgtcagttcc gtatcggttt tttcttcgat caagttcaca atcgtgtctg taggcggtga
     7561 agcatgatgc aaaatattta accaagtgat tttagctttt ccatcttctg tctctgaaga
     7621 actgttactt gaactgctgc cgcatgcggc aagtgctaga attcctgcaa aactaagacc
     7681 taattttacg agcgttgctt ttttcataaa ttttctcctt cactgtttta ataatatttt
     7741 tgttttcttc tgttttttct gtatatgagt tcttccattc caaatggcaa aatatagaat
     7801 ttgctgtcca cgtgactgcg gaaaagaaga gactgccacc gataaaggtt aaaacacctg
     7861 gcattgtttg gatgatcatc agataaatcc ctaccatact taatatcaac actaatgagt
     7921 aatgaagatg tgagaatccg aataaaaatg ccattttcat tttgaaaaaa tatcctttcc
     7981 aatcataatg agccatgatt gggaaaatat agagtgctga gagcacatat atgaacgcag
     8041 caatatacaa agcgatccta agataccaat tgtgtatgaa agaaagatcc acaagtaaaa
     8101 caatcccgat ggcgccatat atccagctca tcagcaaggt ttcacgatag ttttctttaa
     8161 aatatgtgaa gtacgttttc gtcaaaggaa acgtttcttt tgagcgcagc cattttctaa
     8221 tcactgcaac catcgcatac gatgcaggac cgaaagtaaa aactcccaaa cctgcgatga
     8281 ctaaaccgcc ccacagaaaa ttcaaataaa ccaactgtaa aatacgattt agccttccgt
     8341 ttattttatc taatgactgc agcatatctt tccctctctt tctatcttag taaacgccgt
     8401 cttctcctag ctttttggca atcttattag ctaaaattac cataaacaat cctacgaccc
     8461 ctttaaacaa tcccattgct gtactgaaac ttaactgtcc attcttcaat ccagctgtat
     8521 aaatataggt gtcgaaaatt tctgcaacgc ttcgattcaa tgaattcaat aacaagtaca
     8581 tatgctcgaa ccctaagtct aatgtctgac cgatttttag aatcagcaag gtaatgatta
     8641 ctggacgaat tgccggcaat gtaacatgcc atgttttgcg taagcgtcct gctccatcca
     8701 tttctgcagc ttcatacaac tgtgtatcga ctgcagttat cgctgaaagg tagatgatcg
     8761 tagaccagcc caactctttc cagatcactt gtccgatata tactgtgcgt gtccattcag
     8821 gggatgttag aaaactgatt ttttcaaatc ccattgttgc aaggaattca ttcaataccc
     8881 ctccgtcaac attcaaaaat acataagtaa tcgaaacaat gattacccaa gacatgaagt
     8941 gagggatgta aatgatcgtt tgaatcccgt ttttcaatcg tttgttcgtt acttcattta
     9001 atagtaaaga caaaatgatt ggcatcggaa aaaacacgaa gatatttaat ccgaacaaga
     9061 tcaacgtatt tcttaataat gtaaagaatg tcggttctgt aaacaaacga atgaaatgtt
     9121 tgaaaccgac ccaaggactt cccattattc ctaaaaatgg ttgataatct tgaaaagcaa
     9181 taacgagtcc ccccatagga agatacctaa aaatcaagaa ataaaggact cccggcagaa
     9241 tcatcacata taaaaattta ttcgttttga ttcctgcaat tatttttttt cgtcttctga
     9301 gcttttcttc ttttttcgat agcgtcagtt gaaatggttc ttgtttcttt acactttcca
     9361 taagcccctc cttagttttg tgaatgtttg ctgcttatga cttcatgata gataactatc
     9421 tgaattacgt atataatcat ttttatcgta agtaaaaacg cttacatagc aatgattttt
     9481 gtgctataca aaacgataaa gtgtattttt tcacaaataa actacgcata gcaaaccttc
     9541 tatttttttc tatctgacac ttagcatagg aaatgattat atacgtaatc tttcaccgat
     9601 gataaattga acatatagaa acagaaacat ttaatttatt ttgaggagga gcctatctat
     9661 atgaaaaaga aagtctctat aggagaaaga agttttacac ttttcaacaa tacgtttcta
     9721 attttgctag ctttgatctg tatcgttcct tttttaaata tcatcgctac atcttttgct
     9781 tctacacaag aagtcgtcgc gaaaaagttt atcctttttc ctactacctt ttctttagac
     9841 gcgtatcgct acattctttc tacgccgacc atttttagag cacttgctgt ttcgattggt
     9901 gttacaggtg tcggaacgat cgtcagtatg tgtgcaactt cactgatggc ctacggtctt
     9961 tccagaaagt atttgtttgg tcgaggtttc gtcaactttc ttgttgtatt ttctatgttg
    10021 ttcagcgggg ggatgattcc aacgttctta gttgttcgtt ctctagggct ggtcaattct
    10081 tactgggcca tgatcctgcc tgtggctgtt aatgcaatga atatgatcat tatgagaaac
    10141 tttttccaag ccttgccgga tagtttggaa gaatcagcaa aaatggatgg ttgcacagat
    10201 tttggtgtat ttttcaaaat tatgctgcca ttagctttac cgtctattgc tactatctcg
    10261 cttttttatg ctgttactta ctggaacact tatatgactg ctattttgta tatcaacgat
    10321 tcatctaaat ggccgataca gattctttta cgccaaatcg tcattgtatc aagtgggatg
    10381 caagcagaaa gtagtgccgt tgatgtgatt ccaccagcac aaacagttaa aatggctgtt
    10441 atcgttattg caacagtgcc tatgctgatt gtttatcctt ttgttcagaa atattttgtc
    10501 aaaggagcac tcgtaggttc tgtcaaaggg tgattatgta ctgaacgcaa aatggtagtt
    10561 gtgtcagaag tggtcagctt caagaaataa gaaggcattt acgaaaattg cttcttaaat
    10621 ttttgtgaaa tttcggctta tttttcgaag aatctactcc tggaacaccg tgtatcagag
    10681 gttgtgaaag agacgttcag ccttgagcga tgataaaaac tctcacaaac attgcgacga
    10741 gtaacccaca ggagcaataa gaagcgattt tgaaaaatag cttcccatat ttcacaaaat
    10801 tatgccttat tgtcgatagc ctgtctcttg aacgccattt attcgtaaaa aagaactgtg
    10861 acaagacttt tgtcacagtt ctttttgcgt gcttagcctt gaaatttaac tatccataaa
    10921 tacaaatact agggttattc taacttcttt tattgcttga atcagtccac aagaaaaaca
    10981 gcctcttgat taaacggcgt ttatcttgtt gattctgcgg caataaaatc agccagcagc
    11041 aaaacatgaa gtgctgtttt ttgctgtcct cttttattgc ttgaatcaga tcacaagaaa
    11101 aacagcctct tgattcacaa atattttttt cgatattctc ccggcgtaat cgattccttc
    11161 tttttaaaga aacgaataaa attttgcgga tttcgatatt gcaatctttc tgcaatctct
    11221 tttacggaga ggttagtctc tttcaaccat ttttttgcta cctctaaacg atgattttga
    11281 acaaaatcac caaatttttc tcctgtttcc tttttaaata cattacttaa atagttaggg
    11341 ttataatgca acctatcagc gatcacatct aaagacagat cacgatcata ttctgtgtag
    11401 acgatatgca taatattctc cgacaaattt ttaaattcat gatcagtgat tgcttgagag
    11461 cattctgtga ttggcagaac taattgttct aaaacaagtt tttctagttc tttgggatga
    11521 taattgttca gtgcttttac ataaatttgt ttcatgttat caaagttttt tgaatctact
    11581 cccaataact gtcctaattg aacgagttca ttgatcaagc ggaccgaaac tacttcccga
    11641 ctcaaaggat ttttattttg tgcaaacaat gcatcaatca attcatgtac taaattcttc
    11701 acttcctgat ttccagaacg aatggcttca aacaatcgat tttgttcttc tatcggatat
    11761 ctaataagtg ttttttcatg agcagcagga acgatttcct gataaaaaat aattgaactt
    11821 ggaccagtat ttacacgata atacagggct tcttttgccc gatcaaccga ttgcttgctt
    11881 tcattcagcg tttcgaaccg gttactgatg cctatactga tagttaaatt caagtattct
    11941 tttacacgtt tttgaatgag tcggcaatac tccatgactt ttttgcttgc ttctgcatca
    12001 tctttatcca ttcgataaat agtggcttgc atttcttcat tcaaaacgat cggcagcatt
    12061 cgattttcct ttggcacaat ttccgtgatc atgttgttga tcgctaaaag tagcaaatca
    12121 cgctccccac cgtgactatc ttctaacaga tcgatttgaa ccaaagctgt ataatagcag
    12181 tcattttgag aactataacc aaattgctgc aaccgctgat ctagttctcg cctttctact
    12241 cgatttctaa ataaactaag tacaaatagc gtttccagct gtggcttttg ggtaactaag
    12301 tgtgcactta acgcttcttt ttctcctatg atccgactga ctgattgagc gaccaaatct
    12361 agttctttcc cactgaatcc ttttgattta agattcagac gttcctgtat ttgagaaatc
    12421 ggacgggcaa atctgtccga aaataagtag gaaaaaagac caatgagtcc taccagtgtt
    12481 aaagacactg caactagccc gattctcaga ttgcggatca ttgcgccaat tgcactagag
    12541 tcaacttcta atctgtaaat ccaattatta taatctgatt tcagtactat atacgtttta
    12601 tctttttctc ttatcacttt cagctgattg taatctagat ccacgtccgt taatacagtt
    12661 ggttgctctt tattttttac tgtctgagta gtaaaaaggt tgccaacttc gttttcaata
    12721 gaaagtatgt cttctccact ttcaccaaca acttgttcta ttgtatgttt ctctatcgta
    12781 gcaattccca atgcatattt ttctttttca aacattggca aagaaatcac catttcgatt
    12841 cctttgtttt tcttttgcca aaataaatta tttttgttat ttatgtactt ctgttgatac
    12901 ttttcaactt cctcttcggt cagttgactc aacgatccat tgattactcc ccatttacca
    12961 gccaaactga ctaaatcata attattatca tccataacaa tcgtttctat aaaatttagt
    13021 tctttcctga tttcagaata agtggagtaa tctgtatatg tcagcggatg cttgaatatg
    13081 tccgagaaat ttttggtcga accatacgta gaaaacgaat attcaatcgt tctaattttc
    13141 cattcaacgt tagttttaac ttgcctgagc aaattctttt tgtctgttaa ataactcgat
    13201 tcgatcgatt cataggtatt ctggtaaatg aataaactaa atcctatgac aagtacggct
    13261 cctaataaaa acatcggtaa aaatatttta taaaatactg tacttttctt cattttcttc
    13321 ctctcctcct tttaaaataa aaggctttca tttttctgta aaattgatac tcatctaatt
    13381 aaatgagcat tccccgcttt caagaatagc ataattaaaa ataaaaatcc aagtaatttt
    13441 taacgtgtgc aatcttttca tatcaaagat ctattgactt atcgtagtta tcgtaatatg
    13501 atgaatgcgt taacaaaaag tgaaaaagta aggaaaatga tggaatggta acgatcaaag
    13561 atatcgctaa agcagctggc gtttctcata cgacagtttc tcgtgctttg aataacagca
    13621 gtttaataaa aaatgaaaca aaacaaaaaa tacgacaact agctaaggaa atgaattatt
    13681 cgccaaacta tagtgcaaaa ggtttagtga atcaaaaaaa atatctaatc ggactatttt
    13741 tctcaagttt gcataaagga acttcatcaa atttcctagc agattccatt gcaggcatcc
    13801 attcagtatt agacacaaat tacagtttat ctgttgaaag tatcgatgtt gttgatttaa
    13861 cagaaatcaa cctgcaacgc tacgatggac tgatcgttat gagtcaaagt gatgatgatc
    13921 agcctttcat cgactatctc aaagaaaaag aatttccttt cgttgttgtc aatcgtcaca
    13981 tcaatgatcc agcggttatc aacgtcactg ccgatgatgc ggccggtgtt atgcaggcag
    14041 tagattacgg tattggatgc ggacatacga agatcgctta tattggcgga aagaaaaatt
    14101 ttcgttcgac aaaagaacgg gaaaaagggg tattgaaaag tctaaaaaaa gcgagcatcc
    14161 ctgtcaatct agcctatttt ttaacaggag attacagctt agaaagcggc ctccaaaaca
    14221 tggaatacct attatcactt tctgacctgc ctactctggt tttttgcggc aacgacgaca
    14281 tagcgattgg tgcattgaga gctgtacatt tagctggcct ccgtgttccc cagaacattt
    14341 ctctgatcgg gttcgatgat tctccagtcg tctcctatct caatccgccg cttaccaccg
    14401 tacgtaagcc tttgaaagaa atgtgtcaag aaggaacaaa gcttttacta gatttgatcg
    14461 aaggcaggac gatcgaacaa tctaaaattg agctctccac cttgttgatc cttcgtgagt
    14521 cgatcaatta cttgtaaact acaagtaatt ttatataaaa atgttaacgt gtgcataaca
    14581 cagaaaggaa gtttatatga aaaaactgac aaaagaatta gtgaaaaaaa acgaagcacc
    14641 tgtaaaagtt cttcagtttg gtgaaggcaa ttttatgcgt ggatttattg attggcagat
    14701 tcagcagctg aataaccaac aattgtttaa aggtaatgtt gcaatcattc aacctttaga
    14761 acaaggtctt ggaaatatga tgaaccagca agataattta tatacggtca ttttacaagg
    14821 actcagcaat ggcgaaatcg ttgatacttc cgagattatc accgtagtag aacaaatgat
    14881 caatccttat gatgaatggg atgcttatct atctctagca gaaaatgacg acttggccta
    14941 tatcgtctcc aatacaacag aggcaggaat ccaattcgtg gagactgata atttaacaga
    15001 tactccccca cagagcttcc cgggcaaact gacagccttt ttataccgac gtttcaaatt
    15061 aaacaaagct ggctttacca tcattccttg cgaactgatt gacagaaacg gcgaacgtct
    15121 aaaagaaatc attttacgct acgcagaaaa atgggattta gaacctgctt ttgttgagtg
    15181 gttagaaaaa gaaaatgttt tctgttgtag tttagttgat cgcatcgtcc caggttatcc
    15241 tcgcgaagat gcaggtgttt tcaatcaacg gcataactat gaagatcaat tgatggtcaa
    15301 agcagaacca ttcatgcttt gggtgatcga agggcctaaa gaattggaga atcaactacc
    15361 attcaaacag gctggcttga atgtcatcat cacagatgat atgactcctt atcgtgaacg
    15421 taaggtgcat ttgcttaatg gaccgcatac agcgatggtc cctcttgctt tgttggcgga
    15481 attggaaact gtagaagaag taatgaaaga tcctttattc tccgcatatg ttgatcagtt
    15541 attcaatcta gaattgattc ctatgctttc tttacctaaa gatgaattag ccatttatgc
    15601 tgaccaaatc aaagaacgtt tcttgaatcc ttttgcccat cacaaattag aagcgatttc
    15661 attaaatagt gtttcaaagt tcagcacccg tctgcttcca gtgttcaaga agtatatcga
    15721 agaacaaaat caggtaccac cgttaatcac tgtttcacta gctgcgctac ttcttatgta
    15781 tcgaggcgat caagtaaagc cacatgatga tgaaaagacg ataagcgaat ttactgatgc
    15841 ttggtctgat aatggaacag ctattcctcg attattgcaa aacgctgcgc tttggggaga
    15901 agatttgtct caaataccga atgtgacaga tacagttcaa gaaatagctg caaagatcga
    15961 tcaaaaaggt gtccggtcag tattacaaga aatgaaagga acgatggtcc atgcttgaaa
    16021 ccctgcaacc aacaatgaaa ttaaatcgac gagacagtgt ggtcgtcgca ttaaccccta
    16081 ttccaaaaca aacacaactg acgattgatc agcaaacgat caccactctt gaagatatcc
    16141 cgcaaggtca taagattgca ttgggtgatt tgaaagctgg agacaacgtg atcaaatatg
    16201 gctatccaat cggtcatgtg acgacagacg taactgccgg tcaatggctg cacactcata
    16261 atgtaaaaac caatctttct ggtgaattgg attatcgata tgaaaaagat gtccatccaa
    16321 gtcattacct ttttgagaac cgaacatttc aaggttactt gcgaaaaaat ggaaaagtag
    16381 ggattcgtaa tgatttgttt ttagtaccta ccgttgggtg tgtgaatggc attgctgaac
    16441 tgatcgtcaa acagttcaag gaaaaacatc cagatctggg acagtttgac catattacga
    16501 ttttaaagca tccttatggc tgttcgcagc ttggaaaaga tcatcaaaat actcgagaaa
    16561 ttttagcgga cgcagtaaat catcccaatg ccggaggagt tttagtattt ggattaggtt
    16621 gtgagaacaa tacagttcct gaattcaaaa aaattttagg tgaatatgat gaagaacgag
    16681 tgaaatttct agtggctcaa gatgtttacg atgagatcga acaaggtgtg gctctattag
    16741 aagagctttt aacagcggcg gaaaatgacc agcgcatatc agtcccgctc tctaagttga
    16801 atgtcggttt gaaatgcggc ggttctgatg gtctatcggg aataactgct aatcctttac
    16861 ttggtgcatt ttctgactac ttgattgctc aaggaggcag cacgatctta acagaagtgc
    16921 cagaaatgtt tggcgcagaa caagtattga tggcacgagc ggaaaatgaa gaagtttttg
    16981 actcaatcgt tgacttgatc aatgacttca aacagtattt cttgtcttac ggggaaccag
    17041 tttacgaaaa cccatctcct ggtaataaag caggtgggat cactacatta gaagataagt
    17101 ctttaggctg cacgcaaaaa tcaggaagct ctcctgtcgt tgatgtgcta caatatggcg
    17161 aaaaaatcag aaaaccaggg cttagtttat tgcaagctcc cgggaatgat cttgtagctg
    17221 cctctgctct tgcttcatcc gattgccagt tggtcttgtt taccacagga agagggactc
    17281 cgtttggctc atatgtccca acggtcaagg tatctacgaa tacaactctt tttgaacgca
    17341 aaggtcattg gatggatttt aatgcaggcg tcttattaga acaaccaatg gaagtaatct
    17401 tagaatcgtt tgttcaaaaa atcatcgctg tagctagtgg agaagaaacg aaaaatgaac
    17461 aaaatgacgt tcgagaaatt gccattttta aaaacggagt aacactttga ttttcataaa
    17521 acagtataaa aagaaagaag gaagatttat gtttttatct gatgattttt tattaaaaga
    17581 cgattgggcg aaaaagcttt accattccta cgcaaaaaag atgcccatta ttgattatca
    17641 ctgtcactta gatccaaaag aaatttacga aaacaacaat ttcagcaatc tgacagaagc
    17701 ttggttagga ggcgatcatt acaaatggcg cttgatgcga gcttgcggcg tgcctgaaga
    17761 aaaaatcaca ggaaatgctt ctgattttga aaaattttta gcttggtgtc aaaccgtacc
    17821 aaaaattatc ggaaatccgc tttacagttg gtcacatctt gaattgaagc gtttcttcgg
    17881 tattgacttg ttgataaatg aagaaaatgc agagaaaatt tggacacaag caaatgaaat
    17941 tttggcagct cctgattttc gccgtcggga aatcgcaaaa aaatcgaatg tagaagtgat
    18001 ttgtacaaca gatgatcccg cagacgactt gcactatcat cagttgcttg ctcatgaaga
    18061 accagatttg aaggttctac cttcattccg tccagataaa gcactcaata ttgaaaaaga
    18121 aggatttaat gcatgggtca cactactgga agaagcagct gaccaaacga tcaccaccta
    18181 tcaagaactg atagaagtct tagggcagcg gatagaatac ttccatcaac atggatgtcg
    18241 tgtttccgac cacgctttag acatcttacg ctataaagaa gcggatgaaa cagtattaga
    18301 agagattttt caaaaagcac gagctaacaa actattatct tcagatgaaa tcgatgctta
    18361 tcttacagaa acactgatac atttgaccca cttctatcat tctcataatt ggacgatgca
    18421 gctgcacatc catgcttatc gtaactgtaa tacacaaatg ttggaaaaat tagggcccga
    18481 taccggctat gatggaatga atgatctttc tttgactatt cctctgcaaa aattattgaa
    18541 ccgtgcagaa gaaacggatc agttgccaaa gacgatcctt tattcattga atccgaatga
    18601 ctatcctgcg attttagctt tgatgggctg tttccaaaaa gaacagaatg gaaaactgca
    18661 gttaggttct ggatggtggt acaacgatac tcgagctggt atgcgcgatc aattgacaca
    18721 atttgctgat ggtagtgctt tggggaattt tgtcggcatg ctgacagatt caagaagttt
    18781 cctgtcctac actagacatg aatatttccg tcgtgttctt tgtgaatttt taggcgaaat
    18841 ggtcatgcga ggagaagcac ctgaagatga acaactttta ggaagtctag ttcaagcgat
    18901 tagttataca aatgctaaaa attactttgg cttttttgcc taattgatta atagtaacag
    18961 taacgaatca aaaacaggat agtgaaataa cggaatccgt ttatccacgt tttagagaag
    19021 aagacggacc aa
//
LOCUS       DARDHU010000049        18840 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000049 DARDHU010000000
VERSION     DARDHU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.049"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..538)
                     /locus_tag="SDD03_001689"
     CDS             complement(17..538)
                     /locus_tag="SDD03_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG4304136.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="SDD03_001690"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="SDD03_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG4304137.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="SDD03_001691"
     CDS             4618..5505
                     /locus_tag="SDD03_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG4304138.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="SDD03_001692"
     CDS             5502..6671
                     /locus_tag="SDD03_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304139.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="SDD03_001693"
     CDS             6695..8050
                     /locus_tag="SDD03_001693"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG4304140.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="SDD03_001694"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="SDD03_001694"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG4304141.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="SDD03_001695"
     CDS             10336..10626
                     /locus_tag="SDD03_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304142.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="SDD03_001696"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="SDD03_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG4304143.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="SDD03_001697"
     CDS             11667..12326
                     /locus_tag="SDD03_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4304144.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="SDD03_001698"
     CDS             12483..13415
                     /locus_tag="SDD03_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304145.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /locus_tag="SDD03_001699"
     CDS             13569..14858
                     /locus_tag="SDD03_001699"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG4304146.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="SDD03_001700"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="SDD03_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG4304147.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /locus_tag="SDD03_001701"
     CDS             16438..17025
                     /locus_tag="SDD03_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG4304148.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17099..17254)
                     /locus_tag="SDD03_001702"
     CDS             complement(17099..17254)
                     /locus_tag="SDD03_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304149.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17390..18718
                     /locus_tag="SDD03_001703"
     CDS             17390..18718
                     /locus_tag="SDD03_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304150.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         6158 a         3360 c         4059 g         5263 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17101 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17161 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17221 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17281 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17341 tatcattggt tataattaaa ttataaaaag atttgaaaga ggtgagaaaa tgattggcga
    17401 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaaacagga
    17461 aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta tccgctcttt
    17521 cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc
    17581 ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta gttttcgctt
    17641 ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca aagaaaagca
    17701 atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt tcgcctatat
    17761 gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac ggctattgtt
    17821 tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg gggaagtagt
    17881 agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat gtttcacttc
    17941 gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac cgatttttct
    18001 cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat gtatccagct
    18061 ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat cctattttag
    18121 ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc
    18181 attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc agaatgcaca
    18241 aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt atgatatcca
    18301 acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg cgtattttcc
    18361 ttgggttgac gagaatcgat cacgcatgat gtgctataaa gttatcgatc cgactgttgc
    18421 tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag aagtagaggt
    18481 aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc cttcagaatc
    18541 cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta ccttcaaacg
    18601 aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg
    18661 tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa aaatatgagg
    18721 tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca ttattatccg
    18781 tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt ttggtcatgt
//
LOCUS       DARDHU010000050        18741 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000050 DARDHU010000000
VERSION     DARDHU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.050"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            257..532
                     /locus_tag="SDD03_001704"
     CDS             257..532
                     /locus_tag="SDD03_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304151.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="SDD03_001705"
     CDS             545..2617
                     /locus_tag="SDD03_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG4304152.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="SDD03_001706"
     CDS             2782..4668
                     /locus_tag="SDD03_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4304153.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="SDD03_001707"
     CDS             4680..5627
                     /locus_tag="SDD03_001707"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG4304154.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="SDD03_001708"
     CDS             5647..6174
                     /locus_tag="SDD03_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4304155.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="SDD03_001709"
     CDS             complement(6237..6890)
                     /locus_tag="SDD03_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG4304156.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="SDD03_001710"
     CDS             7022..7864
                     /locus_tag="SDD03_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4304157.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="SDD03_001711"
     CDS             8022..8918
                     /locus_tag="SDD03_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4304158.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="SDD03_001712"
     CDS             8921..9634
                     /locus_tag="SDD03_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG4304159.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="SDD03_001713"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="SDD03_001713"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4304160.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="SDD03_001714"
     CDS             10169..10396
                     /locus_tag="SDD03_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG4304161.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="SDD03_001715"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="SDD03_001715"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4304162.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11682)
                     /locus_tag="SDD03_001716"
     CDS             complement(11182..11682)
                     /locus_tag="SDD03_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG4304163.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="SDD03_001717"
     CDS             complement(11961..12359)
                     /locus_tag="SDD03_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4304164.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="SDD03_001718"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="SDD03_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG4304165.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="SDD03_001719"
     CDS             13472..14239
                     /locus_tag="SDD03_001719"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG4304166.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="SDD03_001720"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="SDD03_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG4304167.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="SDD03_001721"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="SDD03_001721"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG4304168.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="SDD03_001722"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="SDD03_001722"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG4304169.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5093 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt t
//
LOCUS       DARDHU010000051        17795 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000051 DARDHU010000000
VERSION     DARDHU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.051"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SDD03_001723"
     CDS             202..1077
                     /locus_tag="SDD03_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304170.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SDD03_001724"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SDD03_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="SDD03_001725"
     CDS             complement(1642..2973)
                     /locus_tag="SDD03_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4304171.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="SDD03_001726"
     CDS             complement(3122..3835)
                     /locus_tag="SDD03_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4304172.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="SDD03_001727"
     CDS             4010..5434
                     /locus_tag="SDD03_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4304173.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="SDD03_001728"
     CDS             5733..6440
                     /locus_tag="SDD03_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG4304174.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="SDD03_001729"
     CDS             6427..6750
                     /locus_tag="SDD03_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG4304175.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="SDD03_001730"
     CDS             complement(6839..7876)
                     /locus_tag="SDD03_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4304176.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="SDD03_001731"
     CDS             complement(8197..9180)
                     /locus_tag="SDD03_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG4304177.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="SDD03_001732"
     CDS             complement(9595..10365)
                     /locus_tag="SDD03_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4304178.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="SDD03_001733"
     CDS             complement(10358..11293)
                     /locus_tag="SDD03_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4304179.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="SDD03_001734"
     CDS             complement(11609..13258)
                     /locus_tag="SDD03_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG4304180.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="SDD03_001735"
     CDS             complement(13375..15978)
                     /locus_tag="SDD03_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG4304181.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="SDD03_001736"
     CDS             complement(15990..17039)
                     /locus_tag="SDD03_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG4304182.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="SDD03_001737"
     CDS             complement(17065..17379)
                     /locus_tag="SDD03_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG4304183.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..>17795)
                     /locus_tag="SDD03_001738"
     CDS             complement(17395..>17795)
                     /locus_tag="SDD03_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4304184.1"
                     /translation="ASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIGK
                     NIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRILA
                     ELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         5451 a         3608 c         2954 g         5782 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcat
//
LOCUS       DARDHU010000052        17383 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000052 DARDHU010000000
VERSION     DARDHU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.052"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(27..725)
                     /locus_tag="SDD03_001739"
     CDS             complement(27..725)
                     /locus_tag="SDD03_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG4304185.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="SDD03_001740"
     CDS             complement(718..2070)
                     /locus_tag="SDD03_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG4304186.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="SDD03_001741"
     CDS             complement(2293..3693)
                     /locus_tag="SDD03_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG4304187.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="SDD03_001742"
     CDS             3854..4591
                     /locus_tag="SDD03_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG4304188.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="SDD03_001743"
     CDS             4716..5195
                     /locus_tag="SDD03_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG4304189.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="SDD03_001744"
     CDS             5192..5959
                     /locus_tag="SDD03_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304190.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="SDD03_001745"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="SDD03_001745"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4304191.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="SDD03_001746"
     CDS             complement(6862..7464)
                     /locus_tag="SDD03_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4304192.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="SDD03_001747"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="SDD03_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="SDD03_001748"
     CDS             8145..8729
                     /locus_tag="SDD03_001748"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG4304193.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="SDD03_001749"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="SDD03_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG4304194.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="SDD03_001750"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="SDD03_001750"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG4304195.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="SDD03_001751"
     CDS             10630..10809
                     /locus_tag="SDD03_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304196.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="SDD03_001752"
     CDS             10831..11856
                     /locus_tag="SDD03_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG4304197.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="SDD03_001753"
     CDS             12031..13275
                     /locus_tag="SDD03_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG4304198.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="SDD03_001754"
     CDS             13428..14375
                     /locus_tag="SDD03_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4304199.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="SDD03_001755"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="SDD03_001755"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG4304200.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="SDD03_001756"
     CDS             complement(15277..15519)
                     /locus_tag="SDD03_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304201.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="SDD03_001757"
     tRNA            15640..15712
                     /locus_tag="SDD03_001757"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="SDD03_001758"
     CDS             complement(15831..16469)
                     /locus_tag="SDD03_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4304202.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="SDD03_001759"
     CDS             complement(16592..17224)
                     /locus_tag="SDD03_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG4304203.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5928 a         3083 c         3447 g         4925 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taa
//
LOCUS       DARDHU010000053        16950 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000053 DARDHU010000000
VERSION     DARDHU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.053"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1152
                     /locus_tag="SDD03_001760"
     CDS             <1..1152
                     /locus_tag="SDD03_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEG4304204.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="SDD03_001761"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="SDD03_001761"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG4304205.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="SDD03_001762"
     CDS             2728..3003
                     /locus_tag="SDD03_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG4304206.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="SDD03_001763"
     CDS             3009..3776
                     /locus_tag="SDD03_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG4304207.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="SDD03_001764"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="SDD03_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG4304208.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="SDD03_001765"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="SDD03_001765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG4304209.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="SDD03_001766"
     CDS             7920..8222
                     /locus_tag="SDD03_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG4304210.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="SDD03_001767"
     CDS             8225..9388
                     /locus_tag="SDD03_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG4304211.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="SDD03_001768"
     CDS             9414..12842
                     /locus_tag="SDD03_001768"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG4304212.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="SDD03_001769"
     CDS             12863..13987
                     /locus_tag="SDD03_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304213.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="SDD03_001770"
     CDS             13977..14414
                     /locus_tag="SDD03_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4304214.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="SDD03_001771"
     CDS             14398..14736
                     /locus_tag="SDD03_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG4304215.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="SDD03_001772"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="SDD03_001772"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG4304216.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="SDD03_001773"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="SDD03_001773"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG4304217.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="SDD03_001774"
     CDS             15893..16936
                     /locus_tag="SDD03_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG4304218.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5744 a         2932 c         3739 g         4535 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa
//
LOCUS       DARDHU010000054        16932 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000054 DARDHU010000000
VERSION     DARDHU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.054"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..587)
                     /locus_tag="SDD03_001775"
     CDS             complement(<1..587)
                     /locus_tag="SDD03_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4304219.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYK"
     gene            complement(835..1671)
                     /locus_tag="SDD03_001776"
     CDS             complement(835..1671)
                     /locus_tag="SDD03_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG4304220.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1682..2611)
                     /locus_tag="SDD03_001777"
     CDS             complement(1682..2611)
                     /locus_tag="SDD03_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG4304221.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2803..3156
                     /locus_tag="SDD03_001778"
     CDS             2803..3156
                     /locus_tag="SDD03_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4304222.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3230..3886)
                     /locus_tag="SDD03_001779"
     CDS             complement(3230..3886)
                     /locus_tag="SDD03_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG4304223.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4112..6046
                     /locus_tag="SDD03_001780"
     CDS             4112..6046
                     /locus_tag="SDD03_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4304224.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6132..7355)
                     /locus_tag="SDD03_001781"
     CDS             complement(6132..7355)
                     /locus_tag="SDD03_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4304225.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7349..9247)
                     /locus_tag="SDD03_001782"
     CDS             complement(7349..9247)
                     /locus_tag="SDD03_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG4304226.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9241..10359)
                     /locus_tag="SDD03_001783"
     CDS             complement(9241..10359)
                     /locus_tag="SDD03_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4304227.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10442..11275)
                     /locus_tag="SDD03_001784"
     CDS             complement(10442..11275)
                     /locus_tag="SDD03_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4304228.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11268..12185)
                     /locus_tag="SDD03_001785"
     CDS             complement(11268..12185)
                     /locus_tag="SDD03_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4304229.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12208..12387)
                     /locus_tag="SDD03_001786"
     CDS             complement(12208..12387)
                     /locus_tag="SDD03_001786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304230.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12412..13536)
                     /locus_tag="SDD03_001787"
     CDS             complement(12412..13536)
                     /locus_tag="SDD03_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4304231.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13719..14627
                     /locus_tag="SDD03_001788"
     CDS             13719..14627
                     /locus_tag="SDD03_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4304232.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14768..16381
                     /locus_tag="SDD03_001789"
     CDS             14768..16381
                     /locus_tag="SDD03_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4304233.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5344 a         3297 c         2800 g         5491 t
ORIGIN      
        1 tttttatatt ttttaattgt gccgtttacg agaatctctt catctttcaa tactaggcct
       61 attttatttt tataacctgg catgctcatt agataaagga tttcatcgta atggatatag
      121 tgatcgctat taccataacg gacagttgtc cacctttttt tcgtagcatg ttgaacagga
      181 tagttttctt tttctttcaa taagtaactt tggatgcttt ttttattttt ttcataagtg
      241 aattctttta tcaaataacc actaggcgta atctttcgat taataacagt tattgcttta
      301 ttcggatgag acgttagata aacaatttta cacttttcat tcttttctct aagcatttcg
      361 cctagttcga taccgctata ataggcgttc aaatcgatgt ctaggaaaaa aatatctgag
      421 gaatagattt cattattttt aatgaattca acaaattgta taacgttcaa tccctgaacg
      481 atctcaaatt tccaaggact taataaaatt ctggattctt ccaaaatttc tactagcatt
      541 ttttcaaaaa ccaaactatc atccaaaata aatatacgcg ttaccataaa aagtacctcc
      601 actcgatatt ttactatcaa aatcatttca tacaactttt caacatacat gttaggaata
      661 aattgtaatt ctcttcacaa tcttttccaa cacctttgaa accgatatca aatactttca
      721 ggatgctgct tctaaaataa atatcaaaca aatgaataca tcatgcaaaa aaagccagac
      781 aaccgacgtc cttgcgtgtc agctgtctga gctatgttta tttattttgt tcttttaaaa
      841 agaacgatac acatactgat ttttcggagc agagactttc tcagcgtttg taacaactac
      901 agatgggatt tcacgcttga atggttgata atagtctgat tggatggttc cttctatttt
      961 ataccattca tcatttttaa ccgttactcc ttccggcata tgcgtcaaca aaccaaagac
     1021 tcctgaatct gccacacaat ggataatgcc gaatcggaac aagaagatat ctgtattgct
     1081 atcgttcggt gtttggtaaa caaatccttc atagctgatt tttttgccaa tgaattcgct
     1141 agggtaatta taaattagct ccataacttc taaatagttt tcatctgtga tcttgatagt
     1201 ttgcttatcc ccatactttt ctttcaactc attcatttgg tctaagtagt ctgatttgtt
     1261 gaaataaata ctggtatccg gctttaagta ttgtgtctgc atctccggat cccctactga
     1321 ttctttactt actgggaaat taaatccttt ggcttctacg atcgtagtgt cgagactaac
     1381 tgttggaaac aacgtcccca cgattactgg caaagctagc agtacataag cgattccacg
     1441 ttgataaggt tttgacaacc catggtcatg atcgtgctcg tgtgcctttt cttctgcttt
     1501 tccgtttttg acccacaaat acagctgaac taaagccaaa agagcagata aaatcattga
     1561 aagaatagct aagtagcgat aatggacatt gatatattga tcgagtttgc cagaaacttg
     1621 caagtacatc atcaattcga aatacccaga taaaatcaaa aaccgcagca tattttccct
     1681 cctaaataaa taatgtgaat ccaataacgc taagggcaat cagtccgatc agcgataaaa
     1741 taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt tttgatatcg accattgggc
     1801 caaagaccaa aaaggcagct acaggtgctg tgccgaacag acttaacaaa gaagatccta
     1861 taaacgcatc tgcttctgag caaagagaca tcgtaaaggc caataacagc atgatgataa
     1921 tggctaaaat ctttgttgag ccgagcgtta gcataattct agttggtaaa taagtctgca
     1981 tcgcagctgc gattaatccg ccaatgatca aataacgacc tgtatcaaaa aattcgtcga
     2041 ttgaatgtgt caacacatgc ccagtttgat ggatcagctg attttttggt tcattcttct
     2101 cactttgttc ttcttcatgg acatgatgat ggtgattttc tgctacttta gacgtgattt
     2161 tttctttcaa tacagcgtct ttttgaaaat aggcaagcca gacaccaatc acaagggcaa
     2221 caaaaaagct gcctatcacc cgccagcttg ccatctgcca agaattgcca aatgcgataa
     2281 aagtggaaaa aatcacgatt ggattgatga tcggagcagt cagcataaaa gcaaacgctg
     2341 tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat cggtacgatg ccgcattcac
     2401 atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat agagaagata cgattttcag
     2461 gaagccatct cttcacacgt tctggggtca aaaatacttg taaggcgcct gagataagac
     2521 agcctaataa tacgaaaggt aatgcttcaa taacgatcga taagaaaatc gtactcatct
     2581 gcaaaaccga atttggaata aaagaaaaca tgagtttcct cctttttatt tctagttcat
     2641 tcgctttgca aatatacgct tttattcaaa aaaaacaagt tgctctgtaa atttttacac
     2701 aactcttatt ttttaccaaa gcacttcaat tgactttttc tgttattccc attaaattaa
     2761 ggtctcatac atatcacttc tatcataaaa gggagaataa aaatgaaaca aaccactaac
     2821 ttgactgaag ttcaagacat actgcaacag tcatccgttg ccgtcattta tctttcgatg
     2881 cctaattgca gtgtttgtca cgctgttcgt ccacgactag aggaacttct cacacattat
     2941 gatattccgg cacttcattt ggatgcattt gaaacaccag aagtagctag taaatttgaa
     3001 gtgctgactg cgccagttgt cctgattttt catcaaggaa aagaagtctt tcggcaagca
     3061 cggttcatcg actttaaaaa aatccgttat ctactagatg aactgacagc agaagatgat
     3121 tccctttctt atgaagaaat cttccgaaca aaataatcgt tgtccaaaca gaaaaaagcg
     3181 attgaacaag tgaaagtttt gccttcattt gttcaatcgc tttttatttt tattctgctg
     3241 tattttcgga atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt tgcaattcat
     3301 tcgtcaaatc aacattttgg atcaatacat ctgacggagc attcaatcga gcagcggtaa
     3361 agttcaagat tcctttgatt cctgcttccg ttaactgatt gactacttct tgtgctcttt
     3421 cagcgggtat cgttaaaatt gctacttcga tttgttggat cttgatttgt tctgacatat
     3481 cttccattgg ataaacgggg ataccgtcta cgatgcgccc aacgatttcc tcattcacat
     3541 caaaagcaca actgatacgt atgctattac tttgatggaa tttgtatttt agtaaagcac
     3601 ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg tttaatgtct
     3661 ttgcaaaaaa gtgcataaga tcttctacat cataaccata cccacgtttt cctaattcgc
     3721 caaaataaga aaaatctcga cggatcgttg cactatctac ttgaacggct tcacttaatt
     3781 cagttgaaga cacttttttc tttccagcat tatttaaaat ccttaaatag cgataataca
     3841 aaggcagtct tttagctgtc gcctttggaa taatatgatc tttcacgaaa tgacctccac
     3901 gtattgtcta attacatctt tgatttctcc atcatagcag tatttatagg aaaaaatcaa
     3961 ttccattgct cagatattgt gaaattttat tctttattca tttatttatc atttctgcaa
     4021 atctcgcagt ataatggaat ttatggtaga ctactaattg aaaaatagtt ttttatttga
     4081 ggagaattca caaagtagaa aagaggattc catgatatta ttacaagcca atcagattgc
     4141 tcgccatttt ggtgcagaag tcttatttga aaacatacat ttagaaatca gtacaggcgc
     4201 tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg actttattaa agatcattgc
     4261 tggaattgaa gcacctgatg aaggaaagat cgctaaaaat aaaacagcga ctctcggata
     4321 cctcgctcag gacacaggac ttgattccaa tgaaacagtt tggaacgaga tgctgaaagc
     4381 ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag ctagagattt tcattagcga
     4441 aggtacgcca gacacacctg cttatagttc ttatttaaaa gaatacgacc ggctgcaaca
     4501 cgatttttca gatcgaaacg gctatggtta tgaaaatgaa attcgttccg tattgcatgg
     4561 gtttaaattc gattcttcat tttatgatca gaaaatcgat actttatcag gcggacagcg
     4621 cacacgttta gctttagctc gcatgctttt gcaacaccct gacattttga ttctagatga
     4681 accaacgaac catttagata tcgagacact ttcctggctt gagaattatc tgcaaagcta
     4741 tactggtgca ttattgattg tttcccatga ccgttatttt ttagataaag tagtcaatga
     4801 agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt cgaaatatct
     4861 agatttgaaa gcagaacagt tagcaagtga atggaaggct tatgaaaagc agcaaacaga
     4921 aatccataaa ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta ccacaaaacg
     4981 agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc ctcaagggga
     5041 tgaaaaacaa gcaaactttc tattcagtat agaaaagcct tctggaaatg tagtcttgca
     5101 aatcgaagat gccgctatcg gatatgataa tgtcacctta tctgaaccaa tcaatctaga
     5161 tatccgtcgt caagaagcga ttgctctagt aggtccgaat ggtatcggga aatcgacgtt
     5221 gctgaaatca atcattcacc agatcccttt tatccgtgga aaagaaacgc tagggacaaa
     5281 tgtcaccatc ggctattacg atcaaggtca agctgatctg catacaaaca agacgatcct
     5341 agcagaattg tgggatgagc atcctaccac tccagaaaaa gagatccgaa gtgctttagg
     5401 tgctttctta ttttctggcg aagatgtaga aaaaccaatc cctctgctaa gtggcggtga
     5461 aaaagcacgg gttgctttag ccaagttagc gatgaacaaa gaaaattttt tgattttgga
     5521 tgaaccaaca aaccatttag atattgacaa caaagaagta ttagaaaatg cattgatcga
     5581 ttatgacggc acactgcttt ttgtttccca tgaccgatat ttcatcaatc ggatcgcgac
     5641 aaaagtcgtc gaactctctg aaaatggcag caaagtctat ctaggagact atgattatta
     5701 tttagagaaa aagcaggaag aagaagaaat cgccgaattg ttggcacaag atgagccaga
     5761 atctgctcct gctcccacga acaaatctga ttattaccaa agcaaagaac agcaaaagct
     5821 tatccgtagc cttcaacgaa aaattactca ggttgaagaa gaaatggcgc ggatcgatga
     5881 atggatcgat cagttggaac aagaaatggt cgcgccagaa aacctggaag atcacgtaaa
     5941 actcaatgag ctgaaccaag atttagaagc tgctagacaa gatcaagaaa caaaattgac
     6001 ggaatgggaa gaactaagct tggaattaga agaatatgaa aattaacgaa aaaaccaaaa
     6061 cagaaaccat tcatgctgtt ttggtttttc tttttttatt ttatcaaaca aaaattccat
     6121 atttaccttt ttcaccaata agaagaccaa ttactagtca ttctcactaa agcttctaaa
     6181 taatagtaat caccccaaat acaaggtgta tttaccccat tatttttggg cttattataa
     6241 acagactgtg ttaaaaagcc atcttcttct tccgttgtag catatctttc tttcaattgt
     6301 cgcataattt tccaaacagc agactggtat aagttctttt ctgttttgtc taatagacct
     6361 gattctatta attctaataa tccacaaacc atgatcacca tactagatgt atctttttct
     6421 tctctgctag aatcatcaaa gatcaaatcc caataacaaa tggtatcttc tggcaaccga
     6481 ttcataaaat aattggctaa tattttgctt aaatcaaaat atttttgttc tttcaaataa
     6541 cgataactta atgcaaaacc gtaaatcccc catgcttggc ctctagccca acaggaatta
     6601 tcactataac cttgatgcgt acttccatat cttggttttc catctttgac atccatataa
     6661 aatgtatgat aagttgaacc atcttctctt actaaataat ctgctgcatt ttgaatatga
     6721 gcctgagcag cttttttata tttttgattt ccagtttctt cactcgccca atacaataat
     6781 ggaagattca tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc actaggatct
     6841 ccccaagctt gtataactcc tgccttctcc caaaaacgct gaagtaatgt ttctgcagca
     6901 tcaattgaag cttccttcat ttttcgattt cccgttattt tataatctgc tacggttgat
     6961 aaagaataca aaaaaccaag atcgtgatga ttagtgataa tttgacagtc aatcctgtgg
     7021 ataaaatcta tatctgcttt ttcagctgtt tgttgaaata aaggatcacc cgtcttctca
     7081 aataacaacc aattaattcc tgtccaaaat ccttctgtcc aaccaatatt gtctcttaat
     7141 ggataacgtc cttttatagg acactcgctt gggaattgat actgactgtt actgatacgt
     7201 ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct cattattcca ttctttttcc
     7261 attaattctt tataacattc atttatccat ttttctggat gtattatctc ttcaaacatc
     7321 actttatcaa gcatttcccc accagccttt agcataattt tagtcttttt ttattgtttt
     7381 tattttggat agttagcccg gttgtcccat caaaatcaat tttgggacga ttgttccata
     7441 cttctaaact ataatcccga tcttgatgag caaccactcc tgttccgagc caatgggttc
     7501 cttttccaaa tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc atcaagtttg
     7561 tattgggcaa acccataata gctgtggatt ttcgtttaat agtatcgtca cttaaacaga
     7621 taatacctga aaagccagct tcactcaccc tatagttttc cttgtcttct tctatgatta
     7681 ttccttcttt ctcaggagaa cattcaatag caaaccctgt ctcatataga acatattctg
     7741 tatctaattc tactttgtgt acgcgaatct gccagccttc aaaaggaata agccaagttc
     7801 tgatttttgc ctgtaaagta ggttcccatg tagaggcaag tacctctttc ttacatacaa
     7861 tgtctcgtac attatttcga acgatgaact gtccataggt tttgatgctc tcattcttaa
     7921 aactggtcgt gatatatgtg tccgctcgct gaatccctag cattgaatcc attgccattt
     7981 ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa taagcaaatt
     8041 ttaaatattt ttcattggtc aatgtatgga aatttggccc taattgtccc gcattcaaaa
     8101 aaaatgtatg tccattatca tgttgtatga gcaaattggc ttctgaaaga agtttagttc
     8161 cttcacgaat aggcataggc atttctttag ccgtccaata ctcatggtct tcaggtagcg
     8221 ccaacaataa aaaaatctta ttcagccata atggagataa cgtactatta tagggttcag
     8281 tcaatgtcaa ttgagggtag gtataaccaa tattcaggat acctttttca tcaaatattt
     8341 ctttttgcat ccaccatctt atattccgat tgactaaccc cttgatttca cctaatttaa
     8401 aaggcaaaag atcggcccac aacatagctg accaaaagct tgaaactgca aaacgataaa
     8461 tcatgcttcg accaaaaggc acatttgcac catcttccgc aaaaaaatga atatagtctc
     8521 tagaaaaatc aatagctcgg ttcttatata tctggctaat gggcttttct ggttctagta
     8581 cgctataaat caaaccataa taatgaaatg caaaaggatt ataataatct tggcgacctc
     8641 ttgaactgtc tcgataccag ccattttgaa gataacaatt ttctatatta tcaaatgctt
     8701 ctttcagtgc tgattcttta actggataac caaatttttg gataattgtt ccgacaatta
     8761 tcttaaaaaa ttgccaatta ccatctgcac attcatattc aaaaatttgc gagaaccaat
     8821 gaataatctg gcttttttct cctgtatcta attttttcca taataattgt tcatgatgat
     8881 ataacgctaa taacattgcg ggcatttcta ccatacgctg gtctctttca tgtatttctc
     8941 cccaataatc gggatgggaa ggatctgttc cagcttttat tcctgataga attaatttca
     9001 accatcgttc atctaattgg tcaattgcgg gccccgctcc ccataaaacc cgagagaatc
     9061 cttccattcc tgcaatttta tttccatatc ctaccccttg atgtatataa tcaatttgtg
     9121 cgccacttgc tcgataaaac ggctggagtg gttgatttat ctttttaaat aagtcaaagt
     9181 aatcctgtct ggtccttaat agtggtactg gatgtttcat tgtattttct cctcttttta
     9241 ctaccaaata gctaattccg actgttctaa tcgaagtaag ccttcaataa aaaaataatc
     9301 agaatagatc agtggcacat tataagtcag ttctctatgg tatgcttctg ctccgcctcc
     9361 aatgatccca tcttcatcca tattccaatt tgcatagtgc ttttctgtct tttggagtat
     9421 attgatagca gcttcatgat acatttttga tcgtacagag gaaacatatc ctgctaattc
     9481 cagaagaccg catgcagcgc atactccagc actggtatca atattcgaat cttttacagc
     9541 aaaatcaatg agaggaatat aaccagtttt ttcgattttc tgaataaaat ttactgctat
     9601 cttttctgct atagctagat agtacggatt ttttgtgtaa cgataactta acgcgaatcc
     9661 atataaagcc catgcatttc ctctgctcca acaagaattc tcattgtatc cttgcccgcc
     9721 aagtaaccga atatatttcc ctgattctgc atcaaattct gcgatatgcc cggttgatcc
     9781 atctttcctc actagatact tagctgttgt atctgcatgc tgccgagcaa ttttagagaa
     9841 cctcggatca ccagtttccc tcgaagccca atacagtaat ggtaaattca tcatactatc
     9901 aataatcatc caaccaggtt tatggtcatt ccatgctact aaatagttcc ctgaaatatt
     9961 atagcgtcca gcaagtaccg tagctgcatg taaccctagt tgtcgactat gtttgttttg
    10021 agactgttga taatgtgcaa tagccgatgg taagaataaa aaacctacat catgatgaac
    10081 gtctgaaaaa tggtacaggg cagattctaa taattgttct tgttgtattg ctgcctcttt
    10141 gaacttttct cgttttgtct tgtgaaagag ctgccataaa ataccactcc aaaaaccatt
    10201 ggtccaccag tcgattcctc tgtcctttat tacgtctgta tattttccgt tgtaagaaat
    10261 atatggaatt ttattcccca ttctgtctaa ttccgcatct atcttttcag aagctacatt
    10321 caaaattttt cttgaccatt ccttcgtctg ttcattcatg gatatcccgc cattctttta
    10381 atttttatga atgtggagtt gctcctagaa cgaactagat ggttaacatt cgttattttt
    10441 tttacttatt tgcagctcca atgtcattct ccttaaattt agactgcgcc cacacataaa
    10501 tcaagaccat tgggatcact gaagttgcta aaattagcat aattgcgcca taattaattg
    10561 gctcacctgt actagaaacc gtcatatttt tgataattaa gggaaccgta tatttatctt
    10621 tatctgaaag catgactaat ggaaatagat attgattcca acttcccata aagataagta
    10681 aaccactagt aataatcgta ggtgataaaa tcggtacagc gattttcgta aaaatcaccc
    10741 actcattggc accatcgatt ctggcagcct ccatcagtgc tgtaggaaat tgactgctat
    10801 attgtcgcat cataaacact gaagttgtac tggcaatcga agggatgatc acacctgcat
    10861 aagtattcga taatcctaaa cgactcataa tgtaaaataa aggaatcaat gtggtaaaaa
    10921 aaggcaccat cctagatagc ataactgtat tgaacaacag tttattcccc ataaaacgat
    10981 actttgctaa tgcataaccc gccattgtag aactggctgt agctaaaact gtactgatta
    11041 cagcaataac aatcgaattc caaataatca agggataatt atatctcgcc atgatggctt
    11101 gccagttttt taatagcata tccccaaact tgaaattgat tccgccactt aaaatttctt
    11161 gattactttg cgttgaagct acaatcatat ataaatatgg catcaaaaat attaaggcac
    11221 caaacacaat aatgacgtac gtaaaaattc tgctttgtat cttttgttta gtcattttta
    11281 tcacctccga atttaaattg aatagccgat aatataacag atacaaacac catcgtccag
    11341 gcaatagcag aggcttttcc aaaatcaacc atttcaaaac ttgttttata taaattgact
    11401 cctacgacca aagctgaatt ttctggtcca ttggaagtcc caaagaatat atttgggata
    11461 ttgaaattac ccaagccatt gatagtggct aaaactgtgg ccattaaaat gattggcttt
    11521 agcatgggga tcgttaataa aaagaattgc cgaaatttcg atgctccatc tatttcagca
    11581 ctttcgtata tctctttaga aatcccttga agaccagcta aaaataaaat agtgttatat
    11641 cctacactaa gccaaatcat cgtgatgatc attgatattc gtatccaaaa aggatttgtt
    11701 aaccactgta caccgttaat tcctagcgca ttcaaaaatc cattgaataa cccagtcgga
    11761 ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg atgtaatggt tggaatataa
    11821 tagacagttc taaaaaatcc ttttcctttt aactgtgctg aattcaataa aacagcaaag
    11881 taaatagcta atagcatcgt tattggaatt gatcctgcca tgataatcaa aacattcgtg
    11941 taagatttaa taaacaatgg atctcggaag atagctagat aattgtcaaa tccaacaaat
    12001 tcaaatgagt agccaatttt cataaaacta aaagcaaacg agataatgat gggaatcaaa
    12061 atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc cataagcact ttttttatga
    12121 gacgtatctg ttgctttttt cttgctgttc tttattgtct tgcttggtaa cgaagattgc
    12181 atcatcgtta agtcctccta atatttttta atcaccagtt aattttacgc cgtacttatg
    12241 agcaaaattt tctgcttctt ttagatatga attttcaaaa tcacctgttt gccaatagtt
    12301 atatgcagcg gtagtcaaat aagacgtagc atctgaataa gtggctggta aaacagtatt
    12361 tgatgtattt tcattgattt tattcaatag ttcatagtat ttttgtcctc cttagtagtc
    12421 aaactttttc tcagctgctt ctgtttgata ggccaatgag ttagatgtag ccacgcccag
    12481 ctccagtgcg ttggctaagc attcttcatc tgtcagcata aatgtaagaa attgttgtgc
    12541 cacttgtgga tttttggcat ctttagggac ataaaaactt gatcctcctg atactgcacc
    12601 ttttcctgga ttttcttttg aaaaaggaat cagtggagcc atgcgccatt tgtcagatgc
    12661 atcaggaaaa ttcaaagaca tattcgtagc ataccagccg ccagcaacaa acattgcaga
    12721 ttcttgaaat gctttctcag catcttgtgc accgtagaaa tgtacaatat ctgagtcgat
    12781 catttctttg attaactttg cagcatgttt cgcttcttta gtattcagac ggatatttcc
    12841 ttcttcatca agcaaagatt gattctgctg ggctagcatg ttgataaaca attcttggtc
    12901 cccagattct ggcaaggcaa ttccaaaact accagttttt tcttttaatg cttttgccac
    12961 ctggatatat tcttcccaag aactgatttc ttcaaaatct acacctattt cagcaaattt
    13021 ttttggttga taataacaaa gtgctggggt aaaatcacta gcccaaggaa taatcgtttc
    13081 attactcgtt tgttgacgca aaatattcaa tttttgatca taaaactgat cgccatattt
    13141 gtcataaaat ccatattcat cagcactata aaaagcatct tgaaattttt ccagcacacc
    13201 tgctgctgaa gtgtctgcca tggagataat atccggcata tcttcgtgtg ttaccaattt
    13261 tggggtgatc atttgtactg ttgctgttcc cgaagctaca ttcacaattt ttaagtcaat
    13321 atttgctcca gttttgcggt tatagatttt tgcagcattt tcaacatagt ttgtcagttt
    13381 gccgtttgcg acatatactt ctaatgtagt ggcttttttt atcattggat ctgcgatgtt
    13441 tgctgttact tcttcgttag tttttccaca tgcacttaag ctgaataata gggtaatgtt
    13501 tagcaaagct atcaccaatt tgtatctttt tttcatgtct atcccttctt tcttttcaag
    13561 ttcttatata aaacgctttc acttatgtat attaacggaa ttagtaacac aaatatttat
    13621 ataaaaaaat attttacttg tcgttttggc ttttcatttt tgttataata gtcattaatt
    13681 ataaatagaa agctttttat ataaaaagag aaaggaaagt ggttcttttg ttaacaaaca
    13741 accaatcctc caatcttgtc aaaaaaacac taacctattc catagatacc catcttcatt
    13801 tatctgctgc tccctatttt cgactactaa taaatgaaag cgctaattta tatgctgaat
    13861 ttgcacataa atctatagaa ctagataatc cttccctttt attcattgat aaacagcaag
    13921 aagtccaaat cttttctact caaaaggaaa cacaaacttt tatcattatc cagttttatt
    13981 ttgatgagca attattagca catattacgg gacaatcttt ttctatagat caagaaataa
    14041 catctatcat tacttttctg aaaaatgaac agttcaaaat tgattatcta aaacatagta
    14101 agccagctat ttcctatgat caggaaatca attctttttt catactatgc caacagatga
    14161 gcattcattt gctttcctta ttagttactt attgtctgca aaaaattcaa ttgtcttcct
    14221 taccaaggca ttatcaacag gaacttgctt tattgacaat accttcaaat gagaaagtca
    14281 ttcctaaaaa aaagcatatt ctttattcag atgaattagt tgatccaatc atacgatatt
    14341 tagaagaaca tctttcagaa aattttttgc ttgaacaatt agcccaaatt ttttttatca
    14401 gtccagccag cttaaaaaaa cattttagaa aagtgaatgg aaaaagtgtt attagctatt
    14461 ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct agaaaatgaa ttaacctata
    14521 cagaaattgc agatatatta ggatttcatt cgatccatca tttttcttca gcttttaaaa
    14581 aatatacagg gttatcccct tcaaagtatt atctatcctt aaaatagaaa aaaattttta
    14641 ggttttatcc acaaattatt tctctatatt ttctgtctat tcaaaataaa caataatttt
    14701 ccggcacaat tatttgctgt tttttaccat aacatagtac gatcaacgta gaaaaggaga
    14761 tgtttttatg aaagttgttg tcattggcgg tgtagctggc ggtccttcat ttgccactcg
    14821 tttccgtcga ttgaatgaag cgcacgaaat cattatctat gaacgcggag aaaatatttc
    14881 ttatgcaagt tgtgctttgc cttattattt aggaggtgtg atcactgacc gtgactcgct
    14941 gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac atcgatgtct ttactaaaca
    15001 cgaagtaaca gcaatcgatc cttctactaa gcgattaaca gttaaagacc tatccacaaa
    15061 tgaagaaata aaaacagatt acgataagtt gatcatctct tctggtgcta gaccagatta
    15121 tccggatatt cccggagttt ttgaagcaga aaacggcttt gtcctccgta gtgtgacgga
    15181 tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca caacatgtcg tcattcttgg
    15241 tgcaggtgtt atgggtctgg aattagctga gaatttcaag catcgcggct tagacgtgac
    15301 tttaatcgat caattgccac aagtcgcttt cccttatgat ccagaaattg ctaatttagt
    15361 ttatgacaaa ttgctgaaag aaggattagc cgttcattta gaaacaagag ttactgagat
    15421 ccgtgataaa ggtcgagaaa tagtattatc agatggctcc gtcctttctg ctgatatgct
    15481 aatttttgct gttggtgttt ctccgaagaa tgaagtggtg aaagcaaccg gcatacaatt
    15541 atcagataca ggtcagatca ttgttgatga ccagttacaa accaatctgc cggacatcta
    15601 tgcgattggc gatattatcg aaacgactag tgtagtgact ggtcagccga tccaaagtat
    15661 gctttccagt gcggccaatc gtcaaggaca tatgctggca gatattttaa atggtacgcc
    15721 tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa atctttgatc atacagcaag
    15781 ttatgcggga atgacagaac atgaattaaa agcatcaggc ataacaaatt ataaaactgt
    15841 ttttatcact ccctttgacc atgcctattt ctatccagaa gctacaagat taaatctaaa
    15901 gctgattttt gatgcagata gcggtcgtat tttaggtgga caagcatttg gagaaaaagg
    15961 tgtcgataaa cggatgggag aactttctgt agcaatcacc ggaaacttga cagtctttga
    16021 tttgcctgat ttggagttgc cttactctcc accgtattct actacccgtg acccgttgaa
    16081 tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc gtagaaacga tcaaagcaag
    16141 tgatataccc gaaaacgatt tgaaagaagc gttcttttta gatatacgtg aacctaataa
    16201 agcaccttct ggcagtatct cggcaacaaa aaatattcca atgaatgagt tacgagatcg
    16261 aatcaatgaa atcccaaaag ataaaaaagt ttacattact ttcagaagag gattgaatac
    16321 ttatacttct gcccgaatct tggcaggttt gggtatcaaa gcgattttga ttgaagaata
    16381 aaaaaacgac cgcggtgggt agccgcggtc taggagttat ttttacttga ggagtaaaaa
    16441 tgaaaaagta tatagggttg ttggttggta tgtatataga atagaagata aatgtgaaga
    16501 aattgtgcat tatttcttca catttattag gttatagttt aagaaagttt aaacattgcg
    16561 caatttaatt attttagctg tttcttttca taaaaaaaca tgttgtgact ctgagacaaa
    16621 tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa tattacgagg catcatatta
    16681 atctatcagc actagtacct aaactagcat ccctttcgaa tattttttct tatataataa
    16741 agtttgcttt cactcctcta atctcaccaa atcgactggc ttgcgttgtg tgatactagg
    16801 aactgattgg tttagaacga cttttctatt gtaatcatat ttttcgccat cctcgttgtc
    16861 ttctaatcct tcaatgaatg atccctaatg tgtgtattaa ataatttaat ttttttgttc
    16921 ctatttattt aa
//
LOCUS       DARDHU010000055        16665 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000055 DARDHU010000000
VERSION     DARDHU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.055"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(157..864)
                     /locus_tag="SDD03_001790"
     CDS             complement(157..864)
                     /locus_tag="SDD03_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG4304234.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(943..1281)
                     /locus_tag="SDD03_001791"
     CDS             complement(943..1281)
                     /locus_tag="SDD03_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG4304235.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1392..2354)
                     /locus_tag="SDD03_001792"
     CDS             complement(1392..2354)
                     /locus_tag="SDD03_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG4304236.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2369..3700)
                     /locus_tag="SDD03_001793"
     CDS             complement(2369..3700)
                     /locus_tag="SDD03_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4304237.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3708..4388)
                     /locus_tag="SDD03_001794"
     CDS             complement(3708..4388)
                     /locus_tag="SDD03_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG4304238.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4607..5086)
                     /locus_tag="SDD03_001795"
     CDS             complement(4607..5086)
                     /locus_tag="SDD03_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG4304239.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5213..6892)
                     /locus_tag="SDD03_001796"
     CDS             complement(5213..6892)
                     /locus_tag="SDD03_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG4304240.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7440..8942
                     /locus_tag="SDD03_001797"
     CDS             7440..8942
                     /locus_tag="SDD03_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4304241.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8955..9617
                     /locus_tag="SDD03_001798"
     CDS             8955..9617
                     /locus_tag="SDD03_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4304242.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9718..9921)
                     /locus_tag="SDD03_001799"
     CDS             complement(9718..9921)
                     /locus_tag="SDD03_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG4304243.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="SDD03_001800"
     CDS             complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="SDD03_001800"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG4304244.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="SDD03_001801"
     CDS             complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="SDD03_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG4304245.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13885..14499)
                     /locus_tag="SDD03_001802"
     CDS             complement(13885..14499)
                     /locus_tag="SDD03_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4304246.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15105..15482)
                     /locus_tag="SDD03_001803"
     CDS             complement(15105..15482)
                     /locus_tag="SDD03_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304247.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15782..16663)
                     /gene="galU"
                     /locus_tag="SDD03_001804"
     CDS             complement(15782..16663)
                     /gene="galU"
                     /locus_tag="SDD03_001804"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG4304248.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4829 a         3592 c         2846 g         5398 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcccttatt ccaaataaat ggtattcaat cagctaactc tcggtattag
      121 ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt tgccgccgaa
      181 caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag
      241 tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca gtagtccgcc
      301 ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa
      361 ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg tagagttcca
      421 gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa agttagctgc
      481 tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt gtaccgcatg
      541 tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg cagttgcttg
      601 agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc cggcaatttg
      661 ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg cttgtgtacc
      721 agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat tgacgtacgc
      781 tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa
      841 accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact ttattttatc
      901 attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct ttttcacaaa
      961 ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat tatgatcgcc
     1021 ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg acgcaccttt
     1081 tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt ttccatttgc
     1141 atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt tccattcgtt
     1201 cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg agccacattc
     1261 tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag cactattgta
     1321 acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa ggcacgtttt
     1381 cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc ttggatctct
     1441 ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc taatggatgg
     1501 cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga acgtaaacgt
     1561 acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc
     1621 actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata tttatcaaga
     1681 atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat attttgataa
     1741 agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct gttcaaagca
     1801 cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg
     1861 gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa ccgtgcagcc
     1921 ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat catctcacta
     1981 cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc attgggatga
     2041 tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat tctgggtgaa
     2101 ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc aacttcttgc
     2161 atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat ttctttttct
     2221 gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc atcaggatca
     2281 gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc tactatttct
     2341 tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga caaacaacga
     2401 ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta gcggaacgtc
     2461 tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg atcagccgca
     2521 aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc accattgttt
     2581 tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt ccaacgccat
     2641 tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga tttccttcac
     2701 ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta tctgcaatct
     2761 gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta atcaagcctt
     2821 ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag atcatttgat
     2881 ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc gtcatcaccc
     2941 gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca atccccatca
     3001 aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct gacaaacgct
     3061 gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct ggcatataga
     3121 tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct cggttgatca
     3181 tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca agatcatcag
     3241 ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact gcggcgccat
     3301 cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt gatcctggcg
     3361 taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta ttcaagacct
     3421 tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg acctcgccaa
     3481 atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc cctactcgtt
     3541 gaattgttga aactagtccg acattttctg catctttgat tgcacggtac gctgttcctt
     3601 cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca acaggtaaac
     3661 tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta cacttccagc
     3721 gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac aaaagattcg
     3781 ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt cttggcacga
     3841 attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc gccaatcggc
     3901 aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact gaaataagca
     3961 gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc gctaggctcg
     4021 cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc atgtacgaat
     4081 tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa tgaatctact
     4141 cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc ttgattttct
     4201 tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat cagcaaccaa
     4261 tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat aaacgggtca
     4321 atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc atgatacgta
     4381 attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa cgatttcatc
     4441 acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat tgatagtaca
     4501 gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa gcagatataa
     4561 tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa ctaattaaat
     4621 cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga aggcttctcc
     4681 cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg tctgctaaaa
     4741 aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg tggggatatc
     4801 ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct ttcgtcactt
     4861 gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg atcaccccag
     4921 ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc cctctttttt
     4981 gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt tgatagctga
     5041 tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat tcactccctt
     5101 tattctatcg tttattataa agcgagggat gctgtattca aaaattattt gtttttcgca
     5161 aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt gtttattttt
     5221 tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg cgttttgttt
     5281 gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg ctccactcaa
     5341 aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg ctttctttca
     5401 tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga ggttttgaaa
     5461 tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg tcttctgaca
     5521 agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat ccatcaatca
     5581 ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta tattccagca
     5641 cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca tgagccaaat
     5701 ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat gttggtttca
     5761 tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg cgcatattct
     5821 cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt ttcgccacga
     5881 tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct tcttcaatct
     5941 tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt tcacccattc
     6001 ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct ttcaacgtaa
     6061 caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg cgaataattc
     6121 gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct gcatccaata
     6181 cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc ccttcccaat
     6241 atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca ggctgaggat
     6301 tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg atttccgcca
     6361 atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta aaatctcccg
     6421 tataaacgat actaccttct tttgttttca gattgatccc cactgaatct ggtatcgtat
     6481 gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta tgctcatcta
     6541 ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc aattttgcta
     6601 actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac aagtaaggaa
     6661 gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca cgatcaatgt
     6721 tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac tcattttcag
     6781 gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg acatacatgt
     6841 tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc acaaacttca
     6901 cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct aaatatatga
     6961 gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga atcagcagga
     7021 aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg gatatacttc
     7081 attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata agtattcagc
     7141 tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat tgttagaaca
     7201 actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt cctctttcat
     7261 ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt gcaataacta
     7321 tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc ataagattta
     7381 agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg atgcaaaata
     7441 tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt tcattgttat
     7501 tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg attcgaagcg
     7561 ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg actttaagtg
     7621 ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt tctaccactg
     7681 aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg gacgcggaaa
     7741 aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc tcagctatgg
     7801 caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt tcagaaacta
     7861 ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg actcaagcag
     7921 actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct gatggaacag
     7981 caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac aatgtagtca
     8041 ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc acaatcacga
     8101 gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat gaatccagcg
     8161 aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat acactttaca
     8221 cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg atagattctg
     8281 ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa gctgagaagc
     8341 tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag tcaatgttgg
     8401 aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg gctgtagccg
     8461 gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg catgagttag
     8521 gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg tttaccgaag
     8581 tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga
     8641 atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa aaccagcaag
     8701 ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc
     8761 aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa ttggaaatca
     8821 atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc agtctgtttt
     8881 ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc ctattaaact
     8941 aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa gatccgcaag
     9001 atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac gctattttag
     9061 gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta gatgcaccaa
     9121 aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta agaagttatc
     9181 gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat ttatcggctc
     9241 tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat tatcgacaag
     9301 ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga
     9361 ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac
     9421 atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact tcacaagata
     9481 tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc atcgtgactc
     9541 atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat aaaaaatttc
     9601 aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa ccatatctgt
     9661 ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa gtggttttta
     9721 ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt
     9781 ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc
     9841 tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca tatctttaga
     9901 acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg atctaccttc
     9961 agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa aaagatagtt
    10021 agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc tttttcttgg
    10081 accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt atccaagata
    10141 cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt caatatacgt
    10201 gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc tttttgcaca
    10261 gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc taggaaatga
    10321 ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt gaaattatct
    10381 aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac ttcaccatct
    10441 gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc accacgtttg
    10501 gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat
    10561 tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag ctttcttttt
    10621 cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc tcctacacga
    10681 gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg gtgatcggat
    10741 actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt gatgatagaa
    10801 tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt ggagattcct
    10861 tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc ttctccgtct
    10921 gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct caatgtgact
    10981 tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg gaaatgacag
    11041 tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac ttccactcct
    11101 tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc aatcagtaca
    11161 tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat tttcatacat
    11221 ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc caaacgatag
    11281 gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt atctactgtt
    11341 tggattactt ttttatttac aggtctccag tactactgca gacctattgc aatggcaatc
    11401 atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg gaatcttgcc
    11461 cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc tgatacgggt
    11521 gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac ttgtctggat
    11581 acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc ttgtcctgaa
    11641 tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca agcaccaatg
    11701 acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt caaagctgaa
    11761 ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat acctactacg
    11821 attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat gtcattgact
    11881 ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc catcacaaag
    11941 aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca aacgatccag
    12001 acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa ttttttattt
    12061 tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta agtcaacgaa
    12121 ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc ggctaatgca
    12181 gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg gattccagca
    12241 atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt tccgacttga
    12301 gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc aatgacaaat
    12361 ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt agcgccaccg
    12421 aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt agttttactg
    12481 ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc aaaagcacct
    12541 ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc tccagatact
    12601 agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt tccccatatt
    12661 ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta ctgatcgtaa
    12721 agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt tttttctccg
    12781 tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg ttctgctgga
    12841 tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc gtaagcataa
    12901 cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc atctttgtat
    12961 tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag
    13021 atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt gaagtcgcgt
    13081 ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct tttttcagta
    13141 attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg acatttccac
    13201 atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc tatgtatgat
    13261 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    13321 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    13381 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    13441 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    13501 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    13561 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    13621 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    13681 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    13741 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    13801 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    13861 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    13921 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    13981 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    14041 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    14101 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    14161 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    14221 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    14281 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    14341 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    14401 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    14461 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    14521 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    14581 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    14641 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    14701 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    14761 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    14821 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    14881 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    14941 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    15001 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    15061 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    15121 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    15181 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    15241 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    15301 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    15361 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    15421 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    15481 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
    15541 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
    15601 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
    15661 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
    15721 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
    15781 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
    15841 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
    15901 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
    15961 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
    16021 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
    16081 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
    16141 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
    16201 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
    16261 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
    16321 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
    16381 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
    16441 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
    16501 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
    16561 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
    16621 ttcctagccc agcggcagga atgactgctt ttttgacctt cactt
//
LOCUS       DARDHU010000056        16339 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000056 DARDHU010000000
VERSION     DARDHU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.056"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1773)
                     /locus_tag="SDD03_001805"
     CDS             complement(<1..1773)
                     /locus_tag="SDD03_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4304249.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVF"
     gene            complement(1985..2881)
                     /locus_tag="SDD03_001806"
     CDS             complement(1985..2881)
                     /locus_tag="SDD03_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4304250.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2874..3554)
                     /locus_tag="SDD03_001807"
     CDS             complement(2874..3554)
                     /locus_tag="SDD03_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4304251.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(3555..4337)
                     /locus_tag="SDD03_001808"
     CDS             complement(3555..4337)
                     /locus_tag="SDD03_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4304252.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(4330..5247)
                     /locus_tag="SDD03_001809"
     CDS             complement(4330..5247)
                     /locus_tag="SDD03_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4304253.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(5411..5734)
                     /locus_tag="SDD03_001810"
     CDS             complement(5411..5734)
                     /locus_tag="SDD03_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304254.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            6522..9749
                     /gene="fss3"
                     /locus_tag="SDD03_001811"
     CDS             6522..9749
                     /gene="fss3"
                     /locus_tag="SDD03_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG4304255.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            9862..10176
                     /locus_tag="SDD03_001812"
     CDS             9862..10176
                     /locus_tag="SDD03_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4304256.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            10189..10563
                     /locus_tag="SDD03_001813"
     CDS             10189..10563
                     /locus_tag="SDD03_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4304257.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            10573..10908
                     /locus_tag="SDD03_001814"
     CDS             10573..10908
                     /locus_tag="SDD03_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG4304258.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            10991..12331
                     /locus_tag="SDD03_001815"
     CDS             10991..12331
                     /locus_tag="SDD03_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4304259.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            12409..13083
                     /locus_tag="SDD03_001816"
     CDS             12409..13083
                     /locus_tag="SDD03_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG4304260.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            13076..13240
                     /locus_tag="SDD03_001817"
     CDS             13076..13240
                     /locus_tag="SDD03_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304261.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            13316..14458
                     /gene="dcm"
                     /locus_tag="SDD03_001818"
                     /pseudo
     CDS             13316..14458
                     /gene="dcm"
                     /locus_tag="SDD03_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            14448..14738
                     /locus_tag="SDD03_001819"
     CDS             14448..14738
                     /locus_tag="SDD03_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304262.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            14997..16181
                     /locus_tag="SDD03_001820"
     CDS             14997..16181
                     /locus_tag="SDD03_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4304263.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            16178..16315
                     /locus_tag="SDD03_001821"
     CDS             16178..16315
                     /locus_tag="SDD03_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG4304264.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         5185 a         2973 c         3283 g         4898 t
ORIGIN      
        1 gaaaacagaa tgaacaagtg tcccatccca atcaattcct tctggattaa cgtagacaca
       61 gattactggc ttttttataa aatctacttc gatatgcggc atagcaaaat cattcatcgc
      121 tgttgaagat agtttttctc ttttgattgc tccctctagt atcccttctt tcgcgtaatt
      181 ttcttttacc aattttttgg taattatatt taaaacgttc acttgattat cctttatttg
      241 gtgggtaaaa aagcaaaatc gtttatctaa taaatttaga aaagcatctt ctgtttgttc
      301 atttaactgt agatactgtt ttacttttct gacatctgcc cctaacatca aaggactaac
      361 aacaataaca ggaaaatttg tattttcaat cggaatcgtg cttataacaa gttcatcagt
      421 gacttttcta tcttgtagtt cattgatgct tagtacatct tctatctcaa ccatatctgc
      481 aaaaccgtta tttattcttt gctctaataa ttttgatgtt ccatatccgc tactacaaac
      541 taatatcact ttcgttttgt caggtttgta acgatccaaa agtgattgaa tatggagtgt
      601 tacgtaagcg atttcatctt cattaaaatg tatacagaat ctctcttcga tttcctctaa
      661 taaatctaca cttatcataa atgacctttt aaaagaatat ttgattttgt ccgtataagg
      721 gttatagata tttactccca atttgagtct tttaactgct gcattgagat gtaatactag
      781 attattgatc aattcttcat caggataaaa ttcatttaat ttaattctta ctaactcttg
      841 tagctcaaca cccatatcta aataggaaag cttcgtttga tctaagtcgt gattcatagc
      901 atttttttca atcgaagtaa tgtgaatgtc aacataagct tgttcatcta ttggcacaat
      961 aattgaaaat gctttttcaa tcattttgat taaaataagt gtattctttg agatttcttc
     1021 ttcagaatta ttactacgtg ataagaaatt gcttgtttca atcctttcta tcgcaatagc
     1081 aaggtggata gctaaagttt gaaattcata atctgtaaat ttcaattgtg tcttattcat
     1141 gtatcgatta agcaattcgg ctactttttt caatatgtcc ttatttaaga aattattcgc
     1201 ttcaccaatg aaattgatat tcaagtctaa gagatctgtt ttactgttag atgctgtaaa
     1261 gccaccccaa taatagttca caacttctga catcaacctt cttttacttt tttcatcagc
     1321 tttgatgcgc acgccatttc gatctgaaac taaggacatc ccatgcttgt ttagtctttt
     1381 tctaacatct ttcattgtgc tttctaccgt cgacctgctc acgtataatt cgtcagcaag
     1441 attttgaatc tttagagaat cattagtatt caataattta gcaaacacat aagttagacg
     1501 gtcctccgga gagtctgcga tattgatttt atgactggat aataaatttc gaagtgtttc
     1561 tttttttcca ataagcgaaa cgcctttttt aggttgaacc aacatttgta catctggcat
     1621 tttttggatt atttctttca aaattaaaat atctttgcta attgttttcg tagatacata
     1681 taattgttct gataattctt tgtaagttgt gtgctcattt tcaatcaaaa agtatagtat
     1741 tttcatttgc cgttcgctaa attcgatgcc cattatcttc acctcctgaa tacagcttaa
     1801 gtgtacaatt attatctcta ttttcataca gaagaaagtt cttgattaat gtagaagttt
     1861 ctaatattct tgattatttc aaatcaataa aaaagcctgc aatatttata tcacaggctt
     1921 ttttctctat taaatatata taagataata catttttcta ctagttctca ctaatcttgt
     1981 ataattaaac cctccgcaac ttgacggaga tggagaagat gcccccgtct aatctagcgg
     2041 tcatctctcc tcccattcct tccaccaatt tttttgacag gtaaagcccg attccgctag
     2101 attccgtatt tgtgcggctg gtattttcgg tataaaaacg ctggaagact ttttcaacag
     2161 aggtttgcgt tcctggtttg ataggattgg ccactgttaa tcgaatgaat ggatcctctt
     2221 ttgaaaatgt cacctttgct tgtccatctg cataacgcaa taaattctgt atgatatttt
     2281 gcagcactcg cagcaacatg agcggatctg ctaatacagt tgtatttatc gagtgttcag
     2341 cgaacgctac ctcgatattt ttctcagaaa attgttcata aaaagagaaa aatacttctt
     2401 ccacctcttt agaaaaagaa agcgtatgga gatccatttc tttttgcttc gtctctattc
     2461 gagcaagatc ataaaactga tccgtcatac tgagtaatct tgcaacagat tgtttgatga
     2521 tcgttaaata cttctcccgt ttctccgtat cctctacctg ttccagtaat tgtacataac
     2581 cgttaatagc agtcaaagga gttctgagat catgagaaag gccgacgatc gatagtctga
     2641 tgttttcttc catttcttga aatgcttgtc ctttttgttc attttcatcc aaccatgtat
     2701 tcagtgcatc tactaggtcc attagattct tttctcgaaa ttcgactgaa agacgaccac
     2761 catattcttg tcgttcagaa agagtggcga ttttcttagt taatctcttg atttcccttt
     2821 gcaggaagaa aataaaactt cccataaaga taacaacgag aagcaacagc catttaatca
     2881 aatctaaacc ccactcccca caccgtatga atatacacag catgtgcctt tgctagcttc
     2941 cctcgaagat tggaaatatg catattcact gttttctcac tatcaaaata tggttcttgc
     3001 caaactgttt cataaagatt tgctcgcgaa aatacttttt tcggatgttc taaaaaaagc
     3061 ttcagcagat cgaattctct tcctgtcaaa tggacagcca caccattcac atttacatcc
     3121 ctcgactctt tgtccagtaa gatttccttg taatagatgc gttgcgacag atcttttact
     3181 tggacagaag tctgatgacg aagttggatg ccgattcgta acaaaagttc ttcgatatca
     3241 aatggtttcg tcacatagtc atccgctcct gctgccaaca aggaaacttt atctctttga
     3301 tcgctttttg ctgaaagaac gatgatcgga acattcgatg tctgtcggat ttctgctaac
     3361 aatgcttcac ctgaaagccc tggaagcatg agatctagaa ggatcaaatc gacaggatat
     3421 gtgcttaaca ataattttcc ttccgtacct gaataagcat caagaagttt gtatctctgt
     3481 tcaagaacct cctccagtaa ggaatgaaca gcttcatcgt cttcgataat caaaatagtt
     3541 tccagcagga ccacctactt aatgtcagat ttttggaaaa taaacattcc tagagctgat
     3601 gataccacga ttgttactag actgaccaga atcattttcg gtgcttgcga ataagttgct
     3661 tgttgcgcaa agtccgtata catgagccac gtttccgtaa accactccgt tagaaattcc
     3721 aagcctttga tttttgcgaa aacaccggga atgaaggccg ttcccatgat ataaagaaaa
     3781 gaaagaagca aactgacact ggtacttcta gtgatgaatg tcaaagccac tggaacagat
     3841 aatgtcccta atgtcagcag aagcaataca ggaaatactt ttagaacata atttccatag
     3901 tttttgattc cctcaacttc accaaatccg aaagcaagtg tgtaagcaag catggctagt
     3961 cccgtgatca aaaccacgcc aagtgttgtg atcaaaagcg taacgagaag tttcgacaca
     4021 taataagtca ttcgattccg tcctaaagat aacgtattgc gaatcgtacc gttttgaaat
     4081 tcttccccaa tataaaagca cgcaaatgct gcgaaagcca gaagaatcca tgcagccatt
     4141 ttttctacca ttaaaatccc tgttgcaccg ttgatgactg tatccgttgc ttggtttaat
     4201 tcattgccgt attttgtatg catataccat tcgttccaag aataaaaaag cggaagtacg
     4261 accatcagtc ctagtgagac ccacatttta cggctcttcc atacttttat tttttcagca
     4321 gttaatagat taaacatgtg gattgcctcc agttaattgt aagaaataat cttccagttt
     4381 ttgcccaact aggtgaatac catccacagg aacttttgct tctaccaatg cttgattgat
     4441 ggctgctact tggtcaattc cttcaaaaag ctggataaca cggctagatt gtacaaaata
     4501 atccctataa ccaagagagt cgagaaccgt tactgcttgt tccgtcgccg aagtgtcaat
     4561 ttttataaat tgacgagatt cttgtgccaa ttcttctttc gataattctt tgatcaagtg
     4621 tccttcatgc aaaataccat agtgagtggc gatctgtgac aattcatcta gcaagtggct
     4681 agaaagcaag acggtgatgc cccgttcttt taccaaacgt tggatgattt ctcgcatttc
     4741 tgccatccct gctggatcca tcccattcgt cggttcatcc aaaatcaaaa attctggatt
     4801 cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata cctaaagaga aattctttgc
     4861 tttctttttc cctgtatttt ccaatcgcat cattttcagc aggtcttcga tttctctatc
     4921 cgacacaccg ccgttcgctg cttggatacg taggttatct cttgcagtta attctggata
     4981 aagagcaggg gtttcaatcg attgacccat acgtcgtctt gctgcctgca aatccgtcgt
     5041 tccaaataat tcgatcgaac cttcatcaat cgagatcaaa cccataatag ttctcataaa
     5101 ggtagactta cccgctccat tttccccaat caatccatag atcattccac gtttgatttc
     5161 gattgatact ttatctaatg ctttggcagc accgtatttt ttggttatac cagttgcttt
     5221 caagaccgtt tcatttccaa aatccatact tatccttctt tcttattgat gagtgaagta
     5281 tagttgacgg gtataaagaa agcagccaga aatgtataaa gaaagtgtaa attttccaat
     5341 tgaatgtcaa aaaaaacaat cggacaatac gtgtctttcc tattatcagg cagtttgtta
     5401 tacgcttgta tcagtttcct gattctttct tttcgaccaa agtcgtttct ctgtattcct
     5461 cttcactgaa aatttttgct tctttcagat caagcttgag taattttacc agaccggtta
     5521 taaggataac tatagcaaga ctccctccca gaaaaattag actgtctcgc agacgtggtt
     5581 gcgttgtctt ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
     5641 tggtaatcgt tgttatcgtt gacataagtc gtccttgtat atgcgttgga acttctcgct
     5701 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
     5761 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
     5821 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
     5881 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
     5941 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
     6001 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
     6061 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
     6121 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
     6181 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
     6241 gttttttctt cgtatcattg aataagtttg aaaaaccttg atttataaag gttttcccgt
     6301 aaatatattt tattatattt gattaaattt gaaatgtttg tgttctaaaa tgtgttctaa
     6361 tttaaaggca gtatatttcg tattttagat taaattttta ggctcattca ttctcaaatc
     6421 cattctattc agtcaataaa aattttaaaa agactttata aacaaagatg ttcaagcgag
     6481 catcttattt ttttgtgctt aatttgaaag gagaacattt catgtataaa aaacaaaaaa
     6541 agaaattatg tgtattaact attttgagtt tggctgttca agcaattgtt ggagcaattg
     6601 ctcctacgat tacttttgct gatgaaatta cccatccaca aacggtaacc gtagaactag
     6661 atttagcaca tcaatatgct gttgaaggaa cttttagtga tggtcgtcca atgtcagaag
     6721 tcactgttcc gcattatgca gtgtacaacg gctttaaaca agatattttc tgtattgaac
     6781 caggagtccc gatttacaat gagttcactc ctggctatga gaaaaacccg ttaccagata
     6841 tgtccgataa agccaaatta gtttccgttt tatggaaaaa agtaggtaca gatgcagata
     6901 ctcatattgt ggcacagaag atgatctggc aagaagttaa tgggtatacc cttcactcaa
     6961 taaaacgatc agatggtagt gccgtaaata tcgcagccat tgaggcaaaa ataaatcaag
     7021 ccattgacga ttatcagaag aaaccaacct tccataatag tacggctaaa acagtattag
     7081 ggcaatcgac tactgtgacg gatacgaata atctgaattt gtcagagttc gatgaggttg
     7141 tggaaaatgc cgcaaacatt gattatcgtg tgaatggtaa tcagttgata atcaccccaa
     7201 atgccaattc aaaagaaagt ggtgtgctga cgcttaagaa atctgctggt accggaacac
     7261 ctgtcgccta taaaaaggct ggccaacaga ctttaatggc cggggcgatt gataaaccta
     7321 atacctacac aatcaaaatt gatgtggaaa cggagggttc tttaaagatc aaaaaagtcg
     7381 ataaagaatc gggtgatgtt gtaccaggaa cggttttcca tttagacttt ggaaaaactt
     7441 tacctgcaaa agacgtgact actgacaaag aaggcattgc tacattggat gagattcctc
     7501 atggtacaaa agtaaccatt accgaaaaat cagtaccggc gccttatacg attgatacca
     7561 cccccatgac taccaccatt aaagcaggcg aaaccattta cgtgacttca aaaaacgcac
     7621 gggaaaaagg gcaaatcatt ctggataaaa gcggagttga aacagggagt gatctttgga
     7681 atgacaatta ttccttagct ggtaatacgt ttgccattcg caaagacagt ccaaccggtg
     7741 aaatcgttca agaaatgacc acggatgaaa acggtcatgc ggaaacacca aaagagattg
     7801 ctaatgcgtt ggaattggga acctattatg tgaccgaaac taaagctagt catgggttcg
     7861 tgaatacgtt caaacctgta aaagtcgaat tgaagtatgc caatcaaacc gtagcccctg
     7921 ttaccagtaa cgtaaaaggg caaaatcaag aagtcactgg cgaaaccact ttgacgaaag
     7981 aagacaaaga tactggggat aaagcacaag gtaaggcagt ctttgagggt gctgaataca
     8041 ccttgtttac tgctaaggat ggaaaagctg ttaaatggag tgaggctttt aaaccagagt
     8101 tagcgaaggg aacgaaaggt tctgatgaaa cggtcacttt gactttagac gaaaagaacc
     8161 aagttgccgt taaacattta gctatcaacg agtattattg gcaagaaaca aaagcgcccg
     8221 aaggatatag ccttgatgaa acaaagtatc ctgtatccat caaaaaggtg gatgatagcg
     8281 aaaaaaatgc cgtgattacc cgaaatgtta cggcaaaaga acaagtgatt cgctttggct
     8341 ttgatttctt taaatttgct ggatcagctg cgggcactac cgaaactggc ttcaatgatc
     8401 tgacctttaa agtgtcgcca ttggaaggga ccaatgaaat cacaggtgcc gaagatgaag
     8461 cgaccacagc ttataacgag caattagggt ttgatggtta tggcaagttt gaaaatcttc
     8521 cttatgggga ttatttactt gaagaagtag aagccccaga aggttttcaa aagattacgc
     8581 cattagaaat ccgttctact tttaaggaaa acaaagaaga ctttgtgaag agtgagtatg
     8641 tctttaccat taccgaacaa gaccaaaaac aaccaattaa aacggtgacc gttccttacg
     8701 agaagctgac gaacaaagca ttttctgtta gtttgaaccg tttaatgctc tatgatttgc
     8761 ctgaggaaga agatagttta acttctcttg cgacttggaa agacggaaac aaagaattaa
     8821 cttctctgga ttctaccgag ctagttgata aattgagtta caatctacat gaaatcaaag
     8881 aagactggta tgtggtagcg caagccattg atgtggatgc gacaaaagct gcccaagaaa
     8941 aagatgaaaa agccaaacca gtggtgattg cagaaacaag cgcaacgttg gcgaacaaag
     9001 agaaaacagg aacttggaaa attcagcata aattaaccgc cgaacaagtt ttaaataaaa
     9061 ctattgtctt attcaactat gtgtatgaaa ataaggaagc ttttgaagca ggcgataagc
     9121 cagtagcgaa ggatgttagc ttgaataacc aagcccaaac tgtcagttgt acagtggaac
     9181 accatgtttc tatccaaaca aaggcccacc tagaaaacgg ttctcagacc tttactcatg
     9241 gtgatgtggt ggatatgttt gatgatgtgt cgattactca tgatgtactg gatggctcaa
     9301 aagaagcttt cgaaacaatt ctgtatgctt tattaccgga tggtacaaac aaagaaattt
     9361 ggaaatctgg caagattgat tatgaagtaa atgataaaga attcaccaaa actgtgcttg
     9421 cgaaaaaagt agataccgga aaatatccag aaggaaccaa gtttactttt gcggaaatca
     9481 attacgataa agatgggaat atcaatggaa aacacaatga agatttgaaa gaaaaatctc
     9541 aaaccttaac accaaaagaa gttccaacca tactaagtac gccgaaacaa ccggaaacac
     9601 caactgttcc aagtggctct caagaatcta gtcccacagt gaagaccttc cctcaaaccg
     9661 gggagaaaaa ttccaatgtt ttactgttca ttggttttac tttgattttt gcgacggcgg
     9721 gctattattt ctggaatcgc cggaactaag gtgatgagat gatacaagta aatcctaaga
     9781 accaacgtgc gcgaccgcct cctaaaaaag tagtcgcgct atttttgtat ccaaaactaa
     9841 caatgaaaag aggaaaagaa gatggaattg aaatttgtcg tgccagatat ggctgaaacc
     9901 tttggaaagt taagttatgc cggtgaagga gaaattataa cagaaggata tggacggaat
     9961 actacggtga ttggtcgcag ttatcatttg tattccagta agcaacgggc cgatgatatt
    10021 gaggtggtag tagctgcgga agccggtgaa aaggattttg atcaagacca accgctaaaa
    10081 gccgtgaatc cccatttggt tgccaaaggc tatgagattg agaatcgtgg gtttactgat
    10141 tacgtgttgt atgttgatga tttagtgaaa gcataggagg aaaagaagat gagattaaca
    10201 gaaggtattg tcgtagattc aggtttaaca tttgggaagt tgcgtttttc cgcattacgc
    10261 cgagaagtac ggaagcaaaa tgaggatgga acaattggta atgaggtaaa agaacgaacc
    10321 tataatttaa aatcctctgc acaagggcga atgattcaag ttagtatccc agctaatgta
    10381 cccttacgcg agtttgctta tgatgcagag gtagagttgg tcaatccaat cgtggatacc
    10441 gttgccaatt atgtttttcg agaaggaact accgttaatt ggttcattaa ggcggatgat
    10501 ttagtcttga agcggcagcc gaatcaagga aatcctacga accaaaatga aagaaagaaa
    10561 taggtgaaga ttatggaaat taatgttgaa catattttag attgtctcga tcaatatgga
    10621 aaaggcgagc tgactgagga acaactcgtt aaagcattaa cctctgatga aaaaatgttt
    10681 ctgattatac atcaaggttt acttggagaa aatagcgaca gtgaagaaga ctctgatgta
    10741 ctagactggt taggagaaga agagtctttc tttatagttg tcgaagtaaa tgaaaactta
    10801 tgtcggcagg cagaatccat tttggaagaa attggtgtgg agatgccgga tgctattgaa
    10861 ggctttttaa atcaattggt tgaaacgaaa caactcccct cctcgtagca gtaaatgact
    10921 agcataactt tttgaaattg cctaatgaaa tcctattggg ctttttcgat tggaggtgga
    10981 aaccatcaaa atgtacaaag gacatcgcat acgcgcagga gatcagcact tggtttatca
    11041 ctttgttctt ggctggctac tcgctctgtt catcggttgg atgagcgtct tttattttca
    11101 agagtttaga caatttgata tttctaagct atcactttct actattgaaa tcgtttggtc
    11161 tataaaagat ttagtatgtc tgcttggaag tctcgctttt tcaggagcta tgatattgct
    11221 ttatattcac ttttttcttg atcattggcg aagcttgtgg catcgacaaa agctagctcg
    11281 gatgattcta gagaaccatt ggtatgaagt gaaacaaact caaagtgaag gctttttcaa
    11341 agatttgaat agtagccgaa ccagagagac tatcagttac ttccccaaaa tctgttatcg
    11401 aatgaaagat ggattactgt ctattcgcgt tcaaatttca cttgggaaat accaagatca
    11461 gcttttaaaa ttggaaaaga agttagaaag cggactgtat tgtgagttag tagaaaagga
    11521 actcaaagac tcctatgtgg agtatacttt actgtatgat atgatcgcca atcgaattgg
    11581 gatagacgaa gtagtggcag aaaatgggac tctgcgattg atgaaaaatc aagtatgggc
    11641 ttatgattct ttaccccata tgttaatcgc tggtggcacg ggtggaggga agacctattt
    11701 cctcctcacc attatcgaag cactattgaa gtcagatgca gaattgttta tcctcgatcc
    11761 caaaaatgca gatttagctg atttaggtac ggtgatgcct catgtttatt cacaaaagga
    11821 agaaatttct gcttgtgtgg aagatttcta tgaacgtatg atggctcgta gcaaggcaat
    11881 gaaagaaatg tctaactaca aaacaggaga gaattatgcc tatcttggac ttccacccaa
    11941 ctttttaatc tttgatgaat acgtggctta tatggagatg ttaacgacga aagaaagtgc
    12001 ggtgattttg aataagctca aacaaatcgt gatgttgggt cgtcaatctg gtttctttct
    12061 gattttggct tgccaaaggc cggatgcgaa atatttagga gacggaatcc gcgatcaatt
    12121 caactttcgc gtggctttgg gtcgtatgag tgaactcgga tattccatga tgtttgggga
    12181 ggtcgaaaaa aacttcttta tgaagcacat caaaggtcga ggttatgtgg atacaggtgg
    12241 cagtgtgatt agtgagtttt atacgccact tgtaccaaaa gggtatgatt tcttagattc
    12301 aattagtaaa ttagaaaaga tgaatgattg agtgatcttc aatactgact ttatacttca
    12361 agtaacgtat aatactttta tatagtagga agaaagggga taattaatat ggaaagtaat
    12421 gattcttatt tagagtatgc aaaagcaaat aaggataagt ttatttctta tattatcaaa
    12481 gggaaatccc ccgaagttgg acgagacgca gtttttatgg cagggagtcc tggggctgga
    12541 aaatcagagg ttgccattgg attagctgag aattatgata accatgtgat tattgatgcg
    12601 gattactttc gcacccagtt tcctgattat aatggaaaaa actctagtct ctttcaaaaa
    12661 gcatcttctt ggttggtaga gcaggcattt agatatgtct tagatcatgg ctattctttt
    12721 attctggatg caacttttgc aatattaagt gctgaaaaaa atatctcgcg ggcgttgaaa
    12781 aaacattttc gagtgacgat tttctatgtt tatcaagacc ctaaaatcgc ttggaaattt
    12841 acacaagaac gtgaaattgc agagggacgt catgtgccga aaaaggcatt tattaacgct
    12901 tttttcaaat caagagagaa cattgcaaaa gtaaagagta aacatccaga tgtattgctt
    12961 catatcataa taaaagatta tacgaataat atttccgaag ttcattatgc tacggataat
    13021 ctagaattat ttttaccact agggtatagt aggaaagatt tggaggttca attacatgac
    13081 tgagaaagaa caagtaacga aaattgtaaa gaaatacaat aagagcattg cggatttatc
    13141 ggaaaatgct actgcaaaag agttcaaaac ggtgattaaa tatgtagccg atcaggccaa
    13201 tgaaaaacaa cggaaattag ttggattaaa taaaaaatag taggacaaat taataatgta
    13261 tgagccaaga gcaaaatgtt cttggctttt ttctttgcaa tgaatgaggt aatgtatgag
    13321 atttttagat ttattcgccg gtattggtgg ctttcgttta ggtatggaac aggcgagcca
    13381 tcattgtatc ggtttttgtg aaattgataa atttgctaga cgtagttata aagcaattca
    13441 tgatacaagt aaggaggtgg aaatgcatga catcacaagt gtatcagatg agtttattca
    13501 atccctcgga ccagtggaca tcctttgcgg cggatttccg tgccaagctt tttcaattgc
    13561 gggaaagcgg caaagatttt ccgatactcg aggtacttta ttctttgaaa tcgctcggtt
    13621 caccactctt ctccgaccta agcttttatt ccttgagaac gtccgaggat tgctcaatca
    13681 cgaaggaggg gctacgttcg agacgatcct ccgaacgctg gatggattgg ggtatgacgt
    13741 ggagtggtag gtgcttaact caaaggccta cattccccaa agccgggagc ggatattcct
    13801 tatcggacat tctcgagacg catgtaccga acaagtattt cctatcattg gaccgtcttc
    13861 aacatctgat caaaacatca gaaatttggt aaatatcaat ccttccaatc gaggaatggg
    13921 tggacaagtc tatggttcag atggcgtagc gccaacctta acaggtgatg aaggaatcaa
    13981 aatcgcactg ccagcaaggg atggaatctc tgtagcagga ctattaccgg gaaagtttga
    14041 acaaggtaat cgagtttatg aagttactgg aaccgccccc accttgtcaa cgaaacaagg
    14101 tggtacaaaa atcatgattc gaaaaaatga aacagaatat caagaggtga agccagaaga
    14161 cagtgtgaat ctggcttttc ctaattccac aagtcgccga ggtcgtctgg gaaaacagag
    14221 tgttcatacc ttactaacag gagaccaaca agcgatcgtc acggatcagt atcaaattcg
    14281 gaagctaaca cctagagagt gttggcgact tcagggcttt cccgattggg cttttgatcg
    14341 agattctcaa gtcaattcag atagtcagtt gtataagcaa gccggaaact ctgtaacagt
    14401 tccggtcatt tttgatattg ccagaagatt aaaagagttg gatgtacatg gtttatgatg
    14461 agttgattgg agaaatttat tgggtgattg gaaagattca gtcagaccca gaattagaag
    14521 aacaattgcg acggttaaaa tttgatattc gcaagaatgg tgtcaaagtc gctggcgatc
    14581 catatttaat gaatgaggag acggatgctc gaattgaaat aaatcaagta attgcagaat
    14641 ttgagcggat tgcggaccaa gcaaaagaac cagatattcg tcaatacttg ttagaaatga
    14701 aagcagagtt ggaagtaaat ggtattacag atgagtaacc taaaccaaca caactgactg
    14761 ttaggcgctg accgcggagg attcgcgaag cagaaatcct ccgtggtgct gtgcctgttc
    14821 atggacggcg ttagccgacc atgatgtctc acccccgtat ctaacagggg ggtacaaaaa
    14881 cacaaaaaat aagtaagcaa ccattaagaa tgcttgttgt atcaaagata gtaagtaatt
    14941 tgtataggtg ttaactcatt tgaaaaagtt aacaccttga atttggaggt gagaacttgg
    15001 cacaacggaa tttagattat cggctattaa aagaccgacg caacgaatac ggcgtttcac
    15061 aaaataaatt ggctacggct tgtggactta gtcggccgta cttaaatcag atagaaaatg
    15121 gtggcgtaac cgcatctata aaaaccatga ggaaaatttt tgaccaactg gaaagcttta
    15181 atcccgattt gcctttgacg ttactgtttg actatgtaag gattcgcttt cccacaacgg
    15241 atgcacgaaa aattattcaa gaaattctcc atttgaaatt tgagtatatg ctccatgaag
    15301 attatgcctt ttactcctat caagaacagt atgtcatggg tgatattgtg gtgatgttgt
    15361 ctcatgaaga agataaaggt gtgcttttag aattaaaggg gcgaggttgc cgacaatttg
    15421 aaactttttt actcgctcaa aagcgtagct ggtacgactt tttcgaggat tgtctaaagg
    15481 ccggtggcgt gatgaaacgc ttggacttgg caatcaatga tcgagtgggg ctattggata
    15541 tacctgagct aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt cgtaccttta
    15601 aaagttatcg ttccggggaa cttttaaagg ctgatgaaaa ggacggcatg ggcaatacct
    15661 tatatatcgg cagtctcaag agtgaggttt atttctgttt gtatgaaaaa gactatgaac
    15721 aatatatcaa gttagggatt ccattagacc aaactgagac gaaaaatcgt tttgagattc
    15781 gactgaaaaa tgaccgtgct tttcatgcga ttcaagattt gttaaaaggt cgtagcattg
    15841 agagcaccac gttttccatt atcaatcgtt acttgcgatt tgcggataaa gtagaaggta
    15901 aaaggcgaac caactggccc ttgaatgaac aatgggggcg ttttattgga cggaatcgga
    15961 aagagattca attaacttcc gagccaaaac cgtataccat tgaacgaacc ttgaattggc
    16021 taggccgaca agtcgcccct acgtggaaaa tggctaagga gttggatcga ttgaatcgga
    16081 caacctatat ccaggatatg gtacagaatg cacgcttatc agaccgacat aaaaagattt
    16141 tggagcaaca aagtatggca attgaaaatt tgattatatg aaaagaggaa tcgtcatggg
    16201 aattgtaaaa gatatttttt tggtatttgg tggcagtatg cttggtgtta ctgtgatgtg
    16261 cttgatgaat gcaagtgcag cagcggatcg agcaatggaa aagaaaggaa agtgacaaga
    16321 tgaatttcgg acaaaattt
//
LOCUS       DARDHU010000057        16225 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000057 DARDHU010000000
VERSION     DARDHU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.057"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1475
                     /locus_tag="SDD03_001822"
     CDS             138..1475
                     /locus_tag="SDD03_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG4304265.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="SDD03_001823"
     CDS             1523..3652
                     /locus_tag="SDD03_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG4304266.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="SDD03_001824"
     CDS             3627..4316
                     /locus_tag="SDD03_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304267.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="SDD03_001825"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="SDD03_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="SDD03_001826"
     CDS             complement(5546..5740)
                     /locus_tag="SDD03_001826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304268.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="SDD03_001827"
     CDS             complement(5790..6230)
                     /locus_tag="SDD03_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG4304269.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="SDD03_001828"
     CDS             complement(6234..7079)
                     /locus_tag="SDD03_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG4304270.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="SDD03_001829"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="SDD03_001829"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG4304271.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="SDD03_001830"
     CDS             complement(8703..9650)
                     /locus_tag="SDD03_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4304272.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="SDD03_001831"
     CDS             complement(9873..10223)
                     /locus_tag="SDD03_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG4304273.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="SDD03_001832"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="SDD03_001832"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG4304274.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="SDD03_001833"
     CDS             11646..11966
                     /locus_tag="SDD03_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4304275.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="SDD03_001834"
     CDS             11988..12452
                     /locus_tag="SDD03_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4304276.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="SDD03_001835"
     CDS             12657..13262
                     /locus_tag="SDD03_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG4304277.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="SDD03_001836"
     CDS             complement(13301..14590)
                     /locus_tag="SDD03_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG4304278.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="SDD03_001837"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="SDD03_001837"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG4304279.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16225
                     /locus_tag="SDD03_001838"
                     /pseudo
     CDS             15700..16225
                     /locus_tag="SDD03_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5134 a         2971 c         3297 g         4823 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttga
//
LOCUS       DARDHU010000058        16078 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000058 DARDHU010000000
VERSION     DARDHU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.058"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            164..1309
                     /locus_tag="SDD03_001839"
     CDS             164..1309
                     /locus_tag="SDD03_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4304280.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="SDD03_001840"
     CDS             1446..1790
                     /locus_tag="SDD03_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG4304281.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="SDD03_001841"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="SDD03_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG4304282.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="SDD03_001842"
     CDS             complement(3485..4084)
                     /locus_tag="SDD03_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG4304283.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="SDD03_001843"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="SDD03_001843"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG4304284.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="SDD03_001844"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="SDD03_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG4304285.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="SDD03_001845"
     CDS             7290..8408
                     /locus_tag="SDD03_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG4304286.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="SDD03_001846"
     CDS             complement(8468..9760)
                     /locus_tag="SDD03_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4304287.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="SDD03_001847"
     CDS             9892..10470
                     /locus_tag="SDD03_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4304288.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="SDD03_001848"
     CDS             10599..10976
                     /locus_tag="SDD03_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG4304289.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="SDD03_001849"
     CDS             10909..12981
                     /locus_tag="SDD03_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG4304290.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="SDD03_001850"
     CDS             complement(13192..14139)
                     /locus_tag="SDD03_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4304291.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="SDD03_001851"
     CDS             14385..14849
                     /locus_tag="SDD03_001851"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG4304292.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="SDD03_001852"
     CDS             complement(14911..15954)
                     /locus_tag="SDD03_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG4304293.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         5255 a         2931 c         3388 g         4504 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaata
//
LOCUS       DARDHU010000059        16071 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000059 DARDHU010000000
VERSION     DARDHU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.059"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..894
                     /locus_tag="SDD03_001853"
     CDS             226..894
                     /locus_tag="SDD03_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG4304294.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="SDD03_001854"
     CDS             complement(1094..1480)
                     /locus_tag="SDD03_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304295.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="SDD03_001855"
     CDS             complement(1452..2834)
                     /locus_tag="SDD03_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG4304296.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="SDD03_001856"
     CDS             complement(2990..4177)
                     /locus_tag="SDD03_001856"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG4304297.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="SDD03_001857"
     CDS             complement(4184..5215)
                     /locus_tag="SDD03_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4304298.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="SDD03_001858"
     CDS             complement(5231..6685)
                     /locus_tag="SDD03_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4304299.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="SDD03_001859"
     CDS             6860..7765
                     /locus_tag="SDD03_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG4304300.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="SDD03_001860"
     CDS             7856..8533
                     /locus_tag="SDD03_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4304301.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="SDD03_001861"
     CDS             8530..8700
                     /locus_tag="SDD03_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304302.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="SDD03_001862"
     CDS             8814..9710
                     /locus_tag="SDD03_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4304303.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="SDD03_001863"
     CDS             complement(9765..11135)
                     /locus_tag="SDD03_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4304304.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="SDD03_001864"
     CDS             11373..12119
                     /locus_tag="SDD03_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4304305.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="SDD03_001865"
     CDS             12116..12955
                     /locus_tag="SDD03_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4304306.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="SDD03_001866"
     CDS             12956..13906
                     /locus_tag="SDD03_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG4304307.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="SDD03_001867"
     CDS             14047..14799
                     /locus_tag="SDD03_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304308.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="SDD03_001868"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="SDD03_001868"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG4304309.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5214 a         3007 c         3231 g         4619 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa t
//
LOCUS       DARDHU010000060        15986 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000060 DARDHU010000000
VERSION     DARDHU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.060"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..187)
                     /locus_tag="SDD03_001869"
     CDS             complement(59..187)
                     /locus_tag="SDD03_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG4304310.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SDD03_001870"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SDD03_001870"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG4304311.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="SDD03_001871"
     CDS             complement(1674..2729)
                     /locus_tag="SDD03_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG4304312.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SDD03_001872"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SDD03_001872"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG4304313.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SDD03_001873"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SDD03_001873"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG4304314.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SDD03_001874"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SDD03_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG4304315.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SDD03_001875"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SDD03_001875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG4304316.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SDD03_001876"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SDD03_001876"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG4304317.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="SDD03_001877"
     CDS             10491..12050
                     /locus_tag="SDD03_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG4304318.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="SDD03_001878"
     CDS             complement(12161..14092)
                     /locus_tag="SDD03_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4304319.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SDD03_001879"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SDD03_001879"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG4304320.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="SDD03_001880"
     CDS             complement(15021..15773)
                     /locus_tag="SDD03_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4304321.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         4121 a         3499 c         2919 g         5447 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggtttt
//
LOCUS       DARDHU010000061        15951 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000061 DARDHU010000000
VERSION     DARDHU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.061"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..809)
                     /locus_tag="SDD03_001881"
     CDS             complement(387..809)
                     /locus_tag="SDD03_001881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG4304322.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="SDD03_001882"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="SDD03_001882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG4304323.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="SDD03_001883"
     CDS             complement(2252..3154)
                     /locus_tag="SDD03_001883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG4304324.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="SDD03_001884"
     CDS             complement(3168..4724)
                     /locus_tag="SDD03_001884"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG4304325.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="SDD03_001885"
     CDS             complement(4747..5289)
                     /locus_tag="SDD03_001885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG4304326.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="SDD03_001886"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="SDD03_001886"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG4304327.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="SDD03_001887"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="SDD03_001887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG4304328.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="SDD03_001888"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="SDD03_001888"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG4304329.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="SDD03_001889"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="SDD03_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10781)
                     /locus_tag="SDD03_001890"
     CDS             complement(10026..10781)
                     /locus_tag="SDD03_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4304330.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="SDD03_001891"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="SDD03_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG4304331.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="SDD03_001892"
     CDS             complement(11238..11516)
                     /locus_tag="SDD03_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG4304332.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="SDD03_001893"
     CDS             complement(11629..12597)
                     /locus_tag="SDD03_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4304333.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="SDD03_001894"
     CDS             12880..13854
                     /locus_tag="SDD03_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG4304334.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="SDD03_001895"
     CDS             13859..14434
                     /locus_tag="SDD03_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4304335.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="SDD03_001896"
     CDS             complement(14501..15640)
                     /locus_tag="SDD03_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG4304336.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5295 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat t
//
LOCUS       DARDHU010000062        15687 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000062 DARDHU010000000
VERSION     DARDHU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.062"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            68..751
                     /locus_tag="SDD03_001897"
     CDS             68..751
                     /locus_tag="SDD03_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG4304337.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="SDD03_001898"
     CDS             753..1589
                     /locus_tag="SDD03_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4304338.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="SDD03_001899"
     CDS             1600..3354
                     /locus_tag="SDD03_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4304339.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="SDD03_001900"
     CDS             complement(3630..3971)
                     /locus_tag="SDD03_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4304340.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="SDD03_001901"
     CDS             complement(4039..6210)
                     /locus_tag="SDD03_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG4304341.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="SDD03_001902"
     CDS             6379..7278
                     /locus_tag="SDD03_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4304342.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="SDD03_001903"
     CDS             complement(7349..8206)
                     /locus_tag="SDD03_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4304343.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="SDD03_001904"
     CDS             complement(8191..10893)
                     /locus_tag="SDD03_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG4304344.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="SDD03_001905"
     CDS             complement(10906..12195)
                     /locus_tag="SDD03_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG4304345.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="SDD03_001906"
     CDS             12345..13391
                     /locus_tag="SDD03_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4304346.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="SDD03_001907"
     CDS             13393..15546
                     /locus_tag="SDD03_001907"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG4304347.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4454 a         3282 c         2887 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DARDHU010000063        15561 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000063 DARDHU010000000
VERSION     DARDHU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.063"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            367..555
                     /locus_tag="SDD03_001908"
     CDS             367..555
                     /locus_tag="SDD03_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304348.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="SDD03_001909"
     CDS             545..805
                     /locus_tag="SDD03_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304349.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="SDD03_001910"
     CDS             846..1073
                     /locus_tag="SDD03_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304350.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="SDD03_001911"
     CDS             1138..1710
                     /locus_tag="SDD03_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304351.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="SDD03_001912"
     CDS             1711..2022
                     /locus_tag="SDD03_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4304352.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="SDD03_001913"
     CDS             2013..2273
                     /locus_tag="SDD03_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304353.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="SDD03_001914"
     CDS             2434..2793
                     /locus_tag="SDD03_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304354.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="SDD03_001915"
     CDS             2790..4433
                     /locus_tag="SDD03_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4304355.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="SDD03_001916"
     CDS             4468..5550
                     /locus_tag="SDD03_001916"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304356.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="SDD03_001917"
     CDS             5594..6766
                     /locus_tag="SDD03_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4304357.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="SDD03_001918"
     CDS             6753..7457
                     /locus_tag="SDD03_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG4304358.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="SDD03_001919"
     CDS             7462..8622
                     /locus_tag="SDD03_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4304359.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="SDD03_001920"
     CDS             8698..8976
                     /locus_tag="SDD03_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG4304360.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="SDD03_001921"
     CDS             8957..9328
                     /locus_tag="SDD03_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG4304361.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="SDD03_001922"
     CDS             9344..9748
                     /locus_tag="SDD03_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304362.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="SDD03_001923"
     CDS             9745..10155
                     /locus_tag="SDD03_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304363.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="SDD03_001924"
     CDS             10216..10803
                     /locus_tag="SDD03_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4304364.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="SDD03_001925"
     CDS             10876..11343
                     /locus_tag="SDD03_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG4304365.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="SDD03_001926"
     CDS             11389..11697
                     /locus_tag="SDD03_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304366.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..>15561
                     /locus_tag="SDD03_001927"
     CDS             11892..>15561
                     /locus_tag="SDD03_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG4304367.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLS"
BASE COUNT         5569 a         2305 c         3375 g         4312 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca t
//
LOCUS       DARDHU010000064        15371 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000064 DARDHU010000000
VERSION     DARDHU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.064"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            83..433
                     /locus_tag="SDD03_001928"
     CDS             83..433
                     /locus_tag="SDD03_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG4304368.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            532..1062
                     /locus_tag="SDD03_001929"
     CDS             532..1062
                     /locus_tag="SDD03_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG4304369.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1216..2367
                     /locus_tag="SDD03_001930"
     CDS             1216..2367
                     /locus_tag="SDD03_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG4304370.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2476..4515)
                     /locus_tag="SDD03_001931"
     CDS             complement(2476..4515)
                     /locus_tag="SDD03_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG4304371.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4573..5130)
                     /locus_tag="SDD03_001932"
                     /pseudo
     CDS             complement(4573..5130)
                     /locus_tag="SDD03_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5669..5875)
                     /locus_tag="SDD03_001933"
     CDS             complement(5669..5875)
                     /locus_tag="SDD03_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304372.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5891..6505)
                     /locus_tag="SDD03_001934"
     CDS             complement(5891..6505)
                     /locus_tag="SDD03_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG4304373.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6652..7335)
                     /locus_tag="SDD03_001935"
     CDS             complement(6652..7335)
                     /locus_tag="SDD03_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG4304374.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7444..7902)
                     /locus_tag="SDD03_001936"
     CDS             complement(7444..7902)
                     /locus_tag="SDD03_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG4304375.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7986..9749)
                     /locus_tag="SDD03_001937"
     CDS             complement(7986..9749)
                     /locus_tag="SDD03_001937"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG4304376.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9970..11430)
                     /locus_tag="SDD03_001938"
     CDS             complement(9970..11430)
                     /locus_tag="SDD03_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304377.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11638..12564)
                     /gene="glsA"
                     /locus_tag="SDD03_001939"
     CDS             complement(11638..12564)
                     /gene="glsA"
                     /locus_tag="SDD03_001939"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG4304378.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12587..14014)
                     /locus_tag="SDD03_001940"
     CDS             complement(12587..14014)
                     /locus_tag="SDD03_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4304379.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14045..15259)
                     /locus_tag="SDD03_001941"
     CDS             complement(14045..15259)
                     /locus_tag="SDD03_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG4304380.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4395 a         3078 c         2648 g         5250 t
ORIGIN      
        1 tttttgaata atcgcttttt cttctctttt tcttatatac tataagtagt aaataatttg
       61 aaaaaaagaa aggtgactga ttatggcgca aaatcgtatt tttacgacaa gttttgcaag
      121 cgtttatcct atgtatgtag caaaagcgga gagaaaagat cgtataaaag aggaagtaga
      181 tgaaatcatt tgttggctga cgggttattc ccaaacagaa ctagaatatc aactacaaaa
      241 acaagtgact ttcgaacaat tttttgcgga agcccctgtc cccaatcctt cacgttcctt
      301 gattacaggt gttgtttgtg gtgtacgagt agaagacatc gaggataact tgatgaaaga
      361 aattcgttat ttagataaaa tgatcgatga gctagcaaaa ggcaaaaaaa tggaaaagat
      421 cctcagaagt tgatttttcc agagatattc ttctccttcg ctcattttgt aacatttaat
      481 ttggtaaaat gactagttca cgttaaaata agtcaaagga ggttttctta tatgtttgag
      541 acgattaaac aaaatattca aaaatacgcc ttattaagag ctgctgttta tattatttct
      601 gggattgcga ttgtgctaaa cccttctgct gtttttcatt ttatcggtta tctgatcacc
      661 gcctatcttg ttttgcttgg gttgttgaat ctttttgagg cttaccgttt taaaaggaaa
      721 acaggttctt ggagtatcgg tctgttcagt ggtctttttt tcctgatctt agctctgatc
      781 gtctatgctt ttgcacctgc aatcgtttct atcctgccga ttcttcttgg attagctgtt
      841 atcatcaaca gcttgttcca gttgttcttc agcatgaata caaaagctaa aagttggagt
      901 gtttatagta gtattctgtt gttgattggc ggattcgtct tattgttcaa tccattcaaa
      961 agtttgatgc ttctattcca aatattcggc tttattttga ttttcatggg catccaggag
     1021 attatcaatt tcatcagaat aagaaaaatg actatacgtt aatcattttt agttattaaa
     1081 atagaccgta taaaaaccaa caatccaaaa aaatcttttg gattgttggt ttttttgtga
     1141 gtatattttc ctattttttc aataatgtat gttatgctaa gttcgggcta tattttatac
     1201 aaaggaatga tgagaatgga atttattggt attttatgcc tgatattgat ttcaacaacg
     1261 attggctcac atatctcgcg gagattaggt atcccagcag tgattggaca attacttgtt
     1321 ggtgttttgc taggccaagc tggattgaat tgggtcaaac ctgatattct agtccatgac
     1381 ttttcggaga ttggtgtgat cttattaatg tttttagctg gtctggaaag tgatttatct
     1441 cttttgaaaa aatatttccg acctggtatg ttcgttgctt tgcttgggat catcttcccg
     1501 gttgtttttg gctggttgac aggcaaagca ttccaagttt cgggaaatga agcgattttc
     1561 tttgggatta ttcttgcggc aacttctgtc agtatttcag tagaagtatt aaaagaacta
     1621 aacgtagtga atacaaaaga aggatcaaca attttgggtg cttctgtggt agatgacatt
     1681 ttagtcgttt tagttctaag tttcagccta tcttttctaa cgggggaagc ttctagcaat
     1741 gcttctttac cgcttgtttt gattgaacag ctaatttatt ttgtttttat tttcttatta
     1801 gtcaaatgga ttgcgccttt cctactttca tttgcagaaa aagtgtacgc aaattcttct
     1861 attatcatcg tatcgctagt tatttgttta ggaatgtctt acgtagcgga tcttgttggg
     1921 cttagctctg tcattggtgc tttttttgct gggatagctg tcagtcagac aaaggttaaa
     1981 gaagaagtat accacaatgt agaggcttta ggatatgcag tatttatacc agtatttttt
     2041 gtcagtgtag gattagaagt tgatttttct gactttagtg aacaactact ctttatttct
     2101 atattgacgc ttgtagcgat tttgaccaaa ttgcttggag gttattttgg tgctcagatt
     2161 gccggttttt ctcaacatag ttcattgatg gtcggtgctg gtatgatttc gcgtggagaa
     2221 atggctttga tcattttaca aattggacag caaagtcaat tgatcgaacc acagtactat
     2281 tcacctttag ttatcgtgat tttattaagt acacttattt ctccacttgt tctcaaatat
     2341 tttacaaaaa aagtttatga agtgtgagta aatcaaaaag aagttgtgaa agagacatcg
     2401 tgaaaaccgt ttatttgaaa aaaggactgt gacgataacg ccacagtcct tttactgtac
     2461 ttaaaaagca attttttatt gataattttg gttgaggtat tgcaatagtt ccgatgctcg
     2521 tttggttgct gaatcatcat cttctttatt ttccaacatg ctgaccatca tataaccttg
     2581 gttatcaaga ttgaaagcaa acaagaaact gttctcttgc ccctttacat cctgtttttc
     2641 tttgatttcc gctgtaccag ttttcgctgc caacggaatc cctaaagcag aaagagaatg
     2701 tgctgtacca tttacatctt gcacaacttc tctcagatct ggcacgatcg tttgtactgc
     2761 tgtttcgctg atgacattct tcttatcttt tgtctcttta tctgcaatca atttaggata
     2821 gacaagtgtg ccattgttgg taaaaacaga atacattgct gcttgctgga taggagtaat
     2881 tagaagttcg ccctgtccat atccagtatc agctagcaag atatctgagt taaagctatc
     2941 ttcattagaa atttgtgctg gattcatact aattggcaaa tcaaggtctt caccaaaaat
     3001 gaatttatct aagcctgtac gaaatttttt ctcacccatt ttcaacgttt cttgtgccgc
     3061 atatatatta tcggaataga tcaaagcagt ttttaagtct acttgtgata catcactact
     3121 gacacgcgtt gttttaaaag atccccaaga actgtctttt tgccatttaa gcccgttgat
     3181 cgtcaacact tcatttggat cgatagtgcc gttgtcgaga ccgattgctg ctgtaatcat
     3241 tttaaacgta gatccaggag cataacctgt cgcaaatcgg ctgatgaatg gatgttcagg
     3301 attttcttca taagctttgt aatcttcttg tgagatcccg tttgtcattt tgtttggatc
     3361 atagcttgga gagctagcaa gcgcaagaag atcaccggtt tttggcgttg tcgcaacagt
     3421 tgatgcagct tttcctccta gactgtcaaa agctattttt tgtgccttcg catcgattgt
     3481 caatttgata tctttgccat tttgaacttc atgttcgatc agaacttttt tctctacacc
     3541 gtctgcatct gtaatgctta atttcccacc tgttgtccca cgaagatcct tatcaaaagc
     3601 catctccaaa cctgagcgac cgatttttcc attactgctt aattctggat tcttatcaat
     3661 atcttctgct gtgatatctc ccacgtaacc aatcagctga gcagctgctt cacccaaagg
     3721 ataatatctg ccgtctactt cttgaatggt agctccagct ggaagttctg gcgttgctcc
     3781 atcaatgatt ttcaatggga caaagtaatc gggttgtacc cagctttgtg aaatcgcctg
     3841 attgatagca tcttctgtta agtcgaagga ggaagcaatc gctttgatat tggctgtttt
     3901 ttcgcctcca tctccaagtt tacttggaac gactcctaat tgttttagtt tgcctgttgt
     3961 tgctaatggt tcaccatttc gatctataat attccctctt gctgcttctt gtgtggtcag
     4021 gcttactttg tcatttcctt ccatttctgg aaaaaccaag ttgggttgcc agttgatcgt
     4081 ggtttgacca tcatttctgt ctaatgttcc tttataagaa agatctttca attcacctaa
     4141 tgaggtattc atctttgctt tatatgaaaa actgtaagta gaatcgtctt ttttatctac
     4201 ttttagattc gatatctgaa gtcctttgac atcagcagca ccgtatatat tctggtattt
     4261 atctaagatc tctttttcag ataatgcact tttatttgcc gccttttttg atgtcatttc
     4321 tgcagctttg ttataatctc ccttgttcaa agcttggaca aattgctcaa ctgtcttttc
     4381 tccagcttct actgcttggg tttcttgata gtgctggtag taaaaataac cgccacccgc
     4441 agctgctatt aagatcactg cgccggcaat ataagcgcct gttcgatttt tgccgtgctt
     4501 gtcacttctt ttcatttttt cctccttctc ttgcttgtca ttcctttatt tcactattta
     4561 tacctgttta ttctaacata tgtctttgag gaaatccata ccacaatcca gaagcacact
     4621 ttctggaagg ttcgtttgga tactaccaag taatttccca accgctttgg gtagcttgaa
     4681 tattgaagtt acgtctttca gctgctgaga gatagctttg atcacttgct gctgccttct
     4741 gatcctcccg aagtctcctt ctttgtcttc ccgaaatcga gcatactgga gtaaagtatt
     4801 accatccatc acttgctctc ctttgtttat atatacgcca tctaaatcta gagatttttc
     4861 tgcatcgatc ttgactccac caggaaacag ttcattaacg caatcgataa aatcttgaaa
     4921 tgtgatactt gcataatagg gagcttcaaa tttgaaattt tcctttaatg tttgatttaa
     4981 taaatcaact cccccataag agtaagctgc atttatctta ttcattccgt aacctggaat
     5041 atcgacatat gaatctctca tgatagagat cagttttgct tgttgtgttt tctggttgta
     5101 ttgtgcaacc agaagtgaat ccgagcgacc tccaccaatc gatctttttt caccgcatct
     5161 cctcctcaca cagcaatcca cttgtcaggt gaggagcgag tttgtctcat caaataagtc
     5221 agtcacaaaa atataaagaa aaggtcagaa accttttcga acaagaattc tgacctagca
     5281 tcatctcatc gtccgagttt tagcactata caacgtaaag aaaacattct tagctattgg
     5341 aaaaagcctt aatccaacag tttctgtttt acccattggc gactttcgac gtttctgggc
     5401 aacagcctat gtttgtatag gagcctcacc taacaaggtt attactatat tctatttacg
     5461 agttcatgat agacttttcc taatgattag gcaatggaat ttgaaatgaa atagtgaaat
     5521 ttaacaatct ttaatatttt ttagaaaaaa ataacaaata agttgaaaga atcccgttgc
     5581 aaattctttc agttgcacca ggattctttg ataatttttt agttttatgt gtttttttat
     5641 atgttttttc acttttcgga ataaatattt atttgttaaa gtaattaaac aatccgctaa
     5701 tcgtttgatc tggcatttct atttccttac catctaaagt aacagtagta acgacaaaat
     5761 ggtcttgcaa tggcggaact tctaagtact gcccttgcag aaaatcattc actgtttcat
     5821 attctttttt catttctttt gaatctgtcg atggtttata gcttattttt aacataacta
     5881 gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc tcctattgct gcataatcat
     5941 ttcctgccaa acggcttaca gcattcaatt ttcttggatc gatccttcct gtcttatcat
     6001 catagactgt ttcatttaaa tgatatttct gtacttctag caaaaataaa tcagctgtta
     6061 ctttattttt attccgtatc tcgacatgtt catataagga tgtttcaaaa cgaacttttg
     6121 cttctgtcaa tgctggtact gaaatgatta ctgaatcagt taaagtcaag tcagtcaggt
     6181 acagttcgct ttcctcatta ggcagattgg ctgctgtttt atttacttct tctacgttgt
     6241 cctcatcaag gatatggata acagcttctc ttgtctcaag aatattcttt gctgtgtctt
     6301 ttactgcatc ttgttttctc tggatcgcca aggataagat gggtcgattc gaactgacga
     6361 tattgaagta actgaagggt gcgatattca ctatatcttt tgctgattta gtcgttacta
     6421 ctgctactgg tctagggatg actgaaccaa tcagcaactt gtaattttca cgttctgtca
     6481 attctgaagg actccgttca aacatggttt cgccttcttt ctataaaatt cgttaaaaag
     6541 cagatcttga agacaaaatg aaaagtaatg cttttttcat cttcttagaa tctgctttaa
     6601 actatctatc aatttttctt ttttgtcaag aatctgtgcc gtgcgtgttt tttagttatt
     6661 tgctttactt gcggcttcga catatctttt agtgatcaat tgcggacggg tgataataga
     6721 tccaactacg actgaaaaag cacctaattc atatgctttc tctacttgtt caggagagtc
     6781 agtatgtcct tccataatta ctgggatatc gacttgttct atgatttgat tcaataaatc
     6841 aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag cctgctaaag tcgtagacaa
     6901 aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca aaagtcgaaa tatctgccat
     6961 agctaaccga ccgtgttcgt gaatcatttg gacaagttca gcagttgttt tttctgtcgg
     7021 acgtttacga gaagttgcat ccagtgcaat gatttctgct tgcgtctcca aaagttcttc
     7081 cacttctttt tctgtcgcag taataaacac ttctgaccca tcgtaatctc ttttgataat
     7141 ccctattacc ggtaaatcaa cgattcgctt gatttcattg atatcttctg tcgtatttgc
     7201 tcgaatacca actgctccgc cttcttttgc agccaaagcc attctcccca taataaaaga
     7261 actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg acgactccgc ctttgatttg
     7321 ttccagtgta tccacaatat ctcctccagt aatgttatat aaaaatacag cttcatataa
     7381 aaatgtaacc cttttctatt caggagacaa taatgagcta tttctttcag cagacctttc
     7441 ttactaaaag aaaattactt caaattcttc gtatacgacc agcttctctt ctgtaaattc
     7501 tatgttcaac gcttctttat aagcatgttt aaaaaactca gcatggactt tccgccaata
     7561 agctaatgtc cgatcccctt ctccttcttt ccaagcatgc tctttcgtga cttcactaaa
     7621 aggcaagatg gtcacattta tatttttgat gatacaaact ggttcatttt ttccattcag
     7681 gaccacgtct aattgaccaa cttttggtaa aggctcattc tcttgtttat acagatcgta
     7741 accacttgat gtaccagtct ttatcttttt tacaactagt tcgctcaact cgtctgccat
     7801 tttttctgta tccccaaatg aaaagaaacc taatttttct gattgataac taggatattt
     7861 ttcttggaac tttttgacat attcttctac ttgctgatac ataacatcca cccctttgct
     7921 ttttataatg aaaaaaccat ccagatacta gtataaacaa ttatctggat ggtgagcata
     7981 atgaattatt gaatattcaa gacacgcaga ctatcttcaa cgatttgttt tggtgttaaa
     8041 tgataacgtt cataaagctg ccctacagga acgctatccg tgaattcttt ctctgcacca
     8101 aaatttaata cgttcattga agaatcacca taataacgac tgatattttc gccaaatcct
     8161 cctgacaaac taccatcttc taatgtgatg actaattcgt gtgtttcttt taatttatct
     8221 aatgtttcta cgtcattttc tgtgatcgtt cctggattga tcaacgttgc ttcgattcct
     8281 ttttttctaa aagcttttac tgtttctttt cctaattcaa gaaaaccgcc taaagctaaa
     8341 attgctactt ttcctccatt atttactgtt tgataggctg gaatactata atcatttgcg
     8401 atagctgtac catgttctac accatgtgta ggaattcgga ttgctacagg ctgcgctgtt
     8461 tgttcgaacg cccaatctag catagagatc atatcctctt ttgttgctgg agcaagatat
     8521 ttgatgttcg ggatacttga taagtaagga atgtcaaatg ttccttggtg tgttcggtca
     8581 ctagctgaga tcgttccgcc accgactaaa ataatcactg gattctcgtt gatcgctaaa
     8641 tcatgtgata attgatcata tgcccgttgc aagaaagtac ttgattggaa gacgactgga
     8701 cgtactccct gagaagccaa agcagaagcg aaagtgatag agtgctgttc agcgatcccc
     8761 acgtcaaaat aatgatctgg atgtttttct tggaatgttt tcagtccaaa ggcgcctggt
     8821 atcgctgcat tgactgcggc aataggagct gtacttgttt gcattttctt atccaaataa
     8881 tccatgatga tcatattaaa tgtttctcca gaagttactt gctttgcctc cccggtggac
     8941 agatcaaatg gtacatgcca atgatatttc attttattat cgattgccgg ctgataaccg
     9001 cgaccttttt ccgtatggac gtgaagtaca atcggatgat caatatcttt tacttcttca
     9061 aataaacgga tcaccgtttc cagatcgttt ccattatcca cgtaacgata atcaaatccc
     9121 attgctttaa ataggttatt gctgctttta ccgttggttt gacgaagctc agctaagttt
     9181 ttatataaac tgccatgatt ctcatcaata gacatctcat tatcgtttac taatacgatt
     9241 agattggatt gtaaaacacc accattgttc aatccttcaa aagctaagcc accacttagg
     9301 gagccgtcac cgataaatgc taccacattt cctgattctt tcttcagatc tctcgcttta
     9361 gctaaacctg ttgctaagct gatagatgta gatgtgtgcc ccactgtaaa atagtcatgt
     9421 ttactttcat gttggttgga atatccgctc acttcacggt aatgtccttt ttcaaaacca
     9481 ttttttcgac cagttaaaag cttatgacca taagactgat gggaaacatc ccaaacgatt
     9541 ttatcctttg gtgaatcaaa tacataatga aacgcaatcg ttaattctac tacacctaga
     9601 ttcggaccaa cgtgcccacc tacatcgctg tccttttgca agattttttg gcgcatctct
     9661 tctgccaact gtttcatttc ctcaacagat aattttttga catctgcggg gctattgatt
     9721 gcatccaata catgtaatac tgtactcata atacattctc tcctccgtac tatcttcttt
     9781 taatgaaaat agatttgtta cactttatct attctactac ttagagttga ctctaagtca
     9841 atagcatttt aaaaaaattt cagagaaagc ttgttatata agtagatctt cgcacgaatc
     9901 aggactcaaa taaaaacctt ctacagacaa ttaaaaagct ttgtagaagg tctcctaatt
     9961 aaggtctcat caagaaatat gtttttttaa taggtgaagt tcctgagcaa tatgatacaa
    10021 atggatgttt gcacctttta atgagattgg atagatcttt gtcttttcag tttctaatcc
    10081 attttgatag ctagcagcat cgcacaaaac cactcttttt ttcgcacata gacattcatc
    10141 aggcaattct tctaatgaac gaaataaaat aggatttatt tcagcgatgc cttcttccgc
    10201 atacaaatga aattgggttc gataaaaaaa ttgtttgact gaactttctg aaacaacaaa
    10261 aatttctatt tcctgtctcc ccgcttgttc actgttgatc aaagaaagac ttttgacgaa
    10321 ttttcgaatg aaattttcat tccatcttag ttggatttga tttttctttt tccactcttc
    10381 caatatttct acaatttttc cgtacagaag ttgttctttt tctgatagaa gatagatcct
    10441 ttccggtaaa aatacttgtc catctgccca caacgtacgg acaaattgta aaaaggattg
    10501 cttgaaaata aaattttctg catcgtcgat ttcagcgata ctaataatct gtaaaatcaa
    10561 ttcattcagt gataggtcat tgagaacaaa atttttataa acaacttcga aatcttttct
    10621 taacagctcc atgttctcat tggtataatt tctactgttg aacaaagaaa aaatataata
    10681 gacatcttct tcttgtaagt ctagaattcc tttttgctct tccaatcctt gacagactaa
    10741 ttgaaataac ggcttcgttt ctgcgctttt cttatcttct tctttcagtc caaggggaaa
    10801 tgcctttctt ccgattgcga tctctattcc tcgtatgata aaggactttt catcttccga
    10861 atatcttctt ttaaaaaaat gagtctctac ataatcaatc aactcgttac aagcttcttc
    10921 tacatacgga tttttatttt catagccttc ccaattgaca taacgtacta aagctagcaa
    10981 gatgttccgc gagtcaaatt ctgggataat gatttcttta tttttcaaat aatcattttc
    11041 tttaaaaaaa tccaatacga tttttttgat tgtatatact ttgctcaaag aaataaattc
    11101 aagatcagcc agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa
    11161 aaaataagat tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa
    11221 tacgagttta tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc
    11281 ggctagactt tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt
    11341 ttgttcgatg tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa
    11401 tatatttcgc tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg
    11461 attttattta atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct
    11521 ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc
    11581 tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta
    11641 ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc
    11701 gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac
    11761 agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa
    11821 ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact
    11881 ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc
    11941 agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc
    12001 gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga
    12061 gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt
    12121 aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc
    12181 tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg
    12241 atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc
    12301 cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac
    12361 gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag
    12421 ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa
    12481 tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa
    12541 ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat
    12601 gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag
    12661 aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa
    12721 caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt
    12781 ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca
    12841 gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg
    12901 acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc
    12961 agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt
    13021 cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag
    13081 gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa
    13141 ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa
    13201 tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc
    13261 caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct
    13321 ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta
    13381 aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt
    13441 tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa
    13501 acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag
    13561 tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg
    13621 ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac
    13681 cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga
    13741 ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt
    13801 tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata
    13861 gcaaaccgcc taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat
    13921 attcatataa tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt
    13981 tttttgcagg aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt
    14041 tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt
    14101 ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat
    14161 aggcatacac ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc
    14221 ttggattcgt tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga
    14281 atccgcccat gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac
    14341 gaaaatcaac agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt
    14401 ttgcttctac ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta
    14461 gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca
    14521 tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc
    14581 gaagtgcacc accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag
    14641 cagctgccat aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa
    14701 gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag
    14761 cgaaatctct aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag
    14821 ggaaataaat cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc
    14881 tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa
    14941 gaaagaacat taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat
    15001 gtctcatcaa aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg
    15061 ccaatccgaa tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt
    15121 gagacatgat cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac
    15181 caggagtcat caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat
    15241 tgataggtaa tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg
    15301 tgtagttgtt tttttcttct gtttttttga attaatactt gcatttataa aagacagaca
    15361 attaataaaa g
//
LOCUS       DARDHU010000065        15188 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000065 DARDHU010000000
VERSION     DARDHU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.065"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..787
                     /locus_tag="SDD03_001942"
     CDS             278..787
                     /locus_tag="SDD03_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4304381.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="SDD03_001943"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="SDD03_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4304382.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="SDD03_001944"
     CDS             2322..3302
                     /locus_tag="SDD03_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4304383.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="SDD03_001945"
     CDS             3424..4842
                     /locus_tag="SDD03_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG4304384.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="SDD03_001946"
     CDS             4913..6382
                     /locus_tag="SDD03_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4304385.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="SDD03_001947"
     CDS             6392..6859
                     /locus_tag="SDD03_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4304386.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="SDD03_001948"
     CDS             6895..7767
                     /locus_tag="SDD03_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4304387.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="SDD03_001949"
     CDS             7848..10382
                     /locus_tag="SDD03_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4304388.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="SDD03_001950"
     CDS             10538..11311
                     /locus_tag="SDD03_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4304389.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="SDD03_001951"
     CDS             11304..12095
                     /locus_tag="SDD03_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4304390.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="SDD03_001952"
     CDS             12105..12596
                     /locus_tag="SDD03_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4304391.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="SDD03_001953"
     CDS             12614..13024
                     /locus_tag="SDD03_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4304392.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="SDD03_001954"
     CDS             13028..14428
                     /locus_tag="SDD03_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG4304393.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="SDD03_001955"
     CDS             14451..14825
                     /locus_tag="SDD03_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4304394.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5272 a         2129 c         3041 g         4746 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatt
//
LOCUS       DARDHU010000066        15073 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000066 DARDHU010000000
VERSION     DARDHU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.066"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(645..1148)
                     /locus_tag="SDD03_001956"
     CDS             complement(645..1148)
                     /locus_tag="SDD03_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG4304395.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1507..2532
                     /locus_tag="SDD03_001957"
     CDS             1507..2532
                     /locus_tag="SDD03_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4304396.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2855..3733
                     /locus_tag="SDD03_001958"
     CDS             2855..3733
                     /locus_tag="SDD03_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG4304397.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4209..4325
                     /locus_tag="SDD03_001959"
     CDS             4209..4325
                     /locus_tag="SDD03_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG4304398.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5253..5591
                     /locus_tag="SDD03_001960"
     CDS             5253..5591
                     /locus_tag="SDD03_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304399.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5685..5915
                     /locus_tag="SDD03_001961"
     CDS             5685..5915
                     /locus_tag="SDD03_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304400.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6315..6686
                     /locus_tag="SDD03_001962"
     CDS             6315..6686
                     /locus_tag="SDD03_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG4304401.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6960..7652)
                     /locus_tag="SDD03_001963"
     CDS             complement(6960..7652)
                     /locus_tag="SDD03_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4304402.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7655..8440)
                     /locus_tag="SDD03_001964"
     CDS             complement(7655..8440)
                     /locus_tag="SDD03_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4304403.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8677..10077
                     /gene="lacG"
                     /locus_tag="SDD03_001965"
     CDS             8677..10077
                     /gene="lacG"
                     /locus_tag="SDD03_001965"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG4304404.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10102..10419
                     /locus_tag="SDD03_001966"
     CDS             10102..10419
                     /locus_tag="SDD03_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4304405.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10432..12105
                     /locus_tag="SDD03_001967"
     CDS             10432..12105
                     /locus_tag="SDD03_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG4304406.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12119..13750
                     /locus_tag="SDD03_001968"
     CDS             12119..13750
                     /locus_tag="SDD03_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG4304407.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            14622..>15073
                     /locus_tag="SDD03_001969"
     CDS             14622..>15073
                     /locus_tag="SDD03_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4304408.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGR"
BASE COUNT         4818 a         2457 c         3068 g         4730 t
ORIGIN      
        1 ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa
       61 atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa
      121 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
      181 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      241 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      301 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      361 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      421 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      481 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      541 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      601 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      661 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      721 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      781 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      841 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      901 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      961 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
     1021 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
     1081 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1141 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1201 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1261 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1321 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1381 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1441 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1501 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1561 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1621 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1681 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1741 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1801 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1861 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1921 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1981 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     2041 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     2101 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2161 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2221 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2281 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2341 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2401 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2461 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2521 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2581 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2641 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2701 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2761 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2821 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2881 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2941 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     3001 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     3061 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3121 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3181 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3241 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3301 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3361 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3421 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3481 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3541 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3601 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3661 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3721 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3781 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3841 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3901 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3961 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     4021 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     4081 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4141 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4201 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4261 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4321 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4381 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4441 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4501 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4561 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4621 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4681 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4741 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4801 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4861 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4921 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4981 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     5041 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     5101 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5161 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5221 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5281 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5341 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5401 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5461 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5521 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5581 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5641 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5701 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5761 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5821 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5881 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5941 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
     6001 cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt aagtagctac
     6061 acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt cttaattagc
     6121 ttattgattc tataatattt ttgatttata taggaaatta aaaatattat agaatcaaca
     6181 ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa atttctcctg
     6241 gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac tttgcggagt
     6301 atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc atcaatcaat
     6361 cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc attatttctt
     6421 cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa
     6481 ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat cttaagttta
     6541 atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa gatacggaaa
     6601 cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact cagcttggtg
     6661 gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa gtatatcaaa
     6721 aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac actattttta
     6781 atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga
     6841 tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt tcactggcag
     6901 ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag ttgaatcgtt
     6961 taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa atctttgatg
     7021 gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc gaatggatca
     7081 attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct
     7141 tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt aagaaaaata
     7201 tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc ccagtcaaac
     7261 cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac agtagccgtg
     7321 gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc aagccctttt
     7381 gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct ttctttttct
     7441 tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc
     7501 tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa accatctttt
     7561 gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc caaaaacatt
     7621 gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg gtattttcgg
     7681 ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga
     7741 tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca taaaaatcat
     7801 ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta tttagagcga
     7861 tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg tgaacgccat
     7921 ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc
     7981 cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt tctggtccat
     8041 ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga attttcttgt
     8101 tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa atcgtttctc
     8161 cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga atctgcagag
     8221 tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt tgagcccctc
     8281 catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg cgaacggtca
     8341 tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct tttttattga
     8401 ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc tctaatttaa
     8461 ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag taaacattta
     8521 gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt ttatgattat
     8581 ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc agttagaaag
     8641 agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc agaagatttt
     8701 atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga aggtggcaaa
     8761 ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc cgatccggca
     8821 agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat
     8881 ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg gaaaccaaat
     8941 ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg
     9001 ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca tggagatttt
     9061 ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt tcatgaattc
     9121 aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac caatcaatat
     9181 ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt cgcctgtctt
     9241 cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg
     9301 ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga cgcgccggaa
     9361 gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt agatgctacc
     9421 tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg tgaagcaaat
     9481 caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt
     9541 aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta cgatggagaa
     9601 aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa agtgaaggga
     9661 atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg gctgatttat
     9721 ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca ttacaacaaa
     9781 atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg cattattatg
     9841 gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa ggcgataaca
     9901 gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt ctcatggaca
     9961 aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca gaagcgttac
    10021 ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa
    10081 gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg ttttgagatc
    10141 gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga agtaagacaa
    10201 ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt aacccttgca
    10261 cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat ggagatggga
    10321 ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag agatctgatt
    10381 caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc tatggatgga
    10441 atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg taatagttat
    10501 ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt ttcaagcatt
    10561 ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga atctgtctcc
    10621 acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact cgtttcagca
    10681 accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc
    10741 aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg
    10801 attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc ttcctttgtc
    10861 gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat taccattaaa
    10921 atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt tcctttctct
    10981 ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt ccaaatctca
    11041 tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga cgggtactta
    11101 ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg
    11161 tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac caatctgcaa
    11221 ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg gaattttgta
    11281 ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct actctttgct
    11341 aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag tttcgctgtc
    11401 aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt tattccattt
    11461 ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg
    11521 aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt ggttctagga
    11581 acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt tgacttcctg
    11641 atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga agcacaaaaa
    11701 gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga agtacctgtg
    11761 gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt actttgtgct
    11821 ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa
    11881 gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct gcaggatttt
    11941 gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa agaagacacc
    12001 gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg attaacgaga
    12061 gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag gagccataat
    12121 gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag gggctttggg
    12181 ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag cagatatttc
    12241 gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata tttcaaaaga
    12301 tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt tgtggcagga
    12361 tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg aaacgacgat
    12421 tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt ttcactacag
    12481 tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt
    12541 tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg aaatgaagca
    12601 gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg gaatgctctg
    12661 gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg ctgctttttt
    12721 gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag taatgctcca
    12781 cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa tcacgagtcg
    12841 aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg gttcaatgaa
    12901 tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt
    12961 tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg cttttggtga
    13021 agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag agtatttgct
    13081 ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct tcttctattg
    13141 ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta tgaattatct
    13201 cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg
    13261 aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa
    13321 aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc aaacctggtt
    13381 tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt ttggggacta
    13441 caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa atgttagtgg
    13501 cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag agtttgatac
    13561 taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg ttgttaaaac
    13621 aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg tttgtgaaat
    13681 tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg tttcattggt
    13741 gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca gccacatcgt
    13801 tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga
    13861 tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac tttgtattat
    13921 gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag
    13981 ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga
    14041 gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag aacttctttt
    14101 tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg gaaccatctt
    14161 atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata gggattcccc
    14221 ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg tataaaacag
    14281 aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac tataaataaa
    14341 aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt atatatgaaa
    14401 actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa cgtcttttat
    14461 atattacttt caaataaata tgaggtagaa tactatctat tttcttttta acccaattgt
    14521 tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc ttctgtagaa
    14581 tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat tttactacag
    14641 aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt gtccatttgg
    14701 agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat tacgaaaagt
    14761 atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac cgtacggtca
    14821 agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt gagttcaagc
    14881 aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc gtcattcacc
    14941 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
    15001 attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa gaggtaacgg
    15061 cgtttaatgg gcg
//
LOCUS       DARDHU010000067        14962 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000067 DARDHU010000000
VERSION     DARDHU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.067"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            30..1160
                     /gene="dnaN"
                     /locus_tag="SDD03_001970"
     CDS             30..1160
                     /gene="dnaN"
                     /locus_tag="SDD03_001970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG4304409.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            1389..1631
                     /gene="yaaA"
                     /locus_tag="SDD03_001971"
     CDS             1389..1631
                     /gene="yaaA"
                     /locus_tag="SDD03_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG4304410.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            1618..2742
                     /gene="recF"
                     /locus_tag="SDD03_001972"
     CDS             1618..2742
                     /gene="recF"
                     /locus_tag="SDD03_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG4304411.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            2739..4685
                     /gene="gyrB"
                     /locus_tag="SDD03_001973"
     CDS             2739..4685
                     /gene="gyrB"
                     /locus_tag="SDD03_001973"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG4304412.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            4705..7176
                     /gene="gyrA"
                     /locus_tag="SDD03_001974"
     CDS             4705..7176
                     /gene="gyrA"
                     /locus_tag="SDD03_001974"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG4304413.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            7412..7711
                     /locus_tag="SDD03_001975"
     CDS             7412..7711
                     /locus_tag="SDD03_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG4304414.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            7761..8273
                     /gene="ssb"
                     /locus_tag="SDD03_001976"
     CDS             7761..8273
                     /gene="ssb"
                     /locus_tag="SDD03_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4304415.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            8299..8535
                     /locus_tag="SDD03_001977"
     CDS             8299..8535
                     /locus_tag="SDD03_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG4304416.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            8678..10651
                     /locus_tag="SDD03_001978"
     CDS             8678..10651
                     /locus_tag="SDD03_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG4304417.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            10657..11109
                     /gene="rplI"
                     /locus_tag="SDD03_001979"
     CDS             10657..11109
                     /gene="rplI"
                     /locus_tag="SDD03_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG4304418.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            11383..12750
                     /gene="dnaB"
                     /locus_tag="SDD03_001980"
     CDS             11383..12750
                     /gene="dnaB"
                     /locus_tag="SDD03_001980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG4304419.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            13530..14822
                     /locus_tag="SDD03_001981"
     CDS             13530..14822
                     /locus_tag="SDD03_001981"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG4304420.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4982 a         2698 c         3235 g         4047 t
ORIGIN      
        1 aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga gctagcttta
       61 tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc cctattttga
      121 caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc aacgctgata
      181 tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta
      241 ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga cttcctgaag
      301 aaacatttac tttagaagtt ttagaaaata aacaagtagc gatcacttct ggaaaagcga
      361 attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt gtcgaaagcc
      421 ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa acagtttttg
      481 ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt ttatctgata
      541 attctttatt agctgtagct actgattctc accgtctaag tcaacgcgtg attccagtag
      601 aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc gaattatctc
      661 gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt
      721 tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat ccagatacca
      781 atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct agctttttag
      841 cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc gttcgtttat
      901 ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg aaagtagaag
      961 aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc aatccggatt
     1021 atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa tttatctctg
     1081 ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt cagctgatta
     1141 ctcctgtacg tacaaattaa caatgatttt caatgttctt gaaaatttaa ttgaaaagaa
     1201 atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc tttctgttta
     1261 tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat atatggataa
     1321 atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat gaaaatgagg
     1381 gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt
     1441 tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca
     1501 ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga
     1561 tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg
     1621 aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt
     1681 tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa
     1741 agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt
     1801 ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt
     1861 ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacag
     1921 aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt
     1981 tctttagtaa aaggcagccc gcagaccagg cggaagtttt tagatatgga attaggccag
     2041 atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac
     2101 cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg
     2161 tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag
     2221 cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg
     2281 gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa
     2341 gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg
     2401 acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact
     2461 tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat
     2521 ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt gatgagcgag
     2581 ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc
     2641 ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt
     2701 tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag
     2761 tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg
     2821 ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg
     2881 tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc
     2941 atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg
     3001 tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg
     3061 ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat
     3121 cttctgttgt taacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg
     3181 tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa
     3241 cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa
     3301 ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg
     3361 ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt
     3421 atgacggcgg gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc
     3481 cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat
     3541 atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag
     3601 gcggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc
     3661 gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag
     3721 gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa
     3781 caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca
     3841 tgaaattctt gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa ggattactag
     3901 cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt
     3961 tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat
     4021 gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc
     4081 gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca
     4141 tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg ggtacagggt
     4201 ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc atgactgatg
     4261 ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat cgttacatgc
     4321 gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac ggggtaaaac
     4381 aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag
     4441 tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa ggacttggag
     4501 aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg atgatggccc
     4561 gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg atgggggatc
     4621 gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag aacttagata
     4681 tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat ccatgatgtc
     4741 aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt tattgtcgca
     4801 cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat tttatacggc
     4861 atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag aatcgtcgga
     4921 gatgtcatgg gtaaatacca tcctcatgga gactatgcga tctatgagtc aatggtacgg
     4981 atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa ctttggttct
     5041 gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag caaaatcgct
     5101 acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa ctatgatgat
     5161 acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt gaacggaaca
     5221 acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc tgaggtagta
     5281 gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt gatggaagta
     5341 ttacctggac cagattttcc aacaggtggt ttggttatgg gaaaatcagg aatccgaaga
     5401 gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat tactgagatg
     5461 ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa taaagcaaaa
     5521 ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat cacagatttg
     5581 cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg tgacgccagc
     5641 gcatctgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc atttggattc
     5701 aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca aattcttgaa
     5761 aactatatcg aacatcagcg cgaagtcatc acaagacgga cagtctttga aaaagaaaaa
     5821 gcagaagcaa gggcacacat tttagaaggt ttgcgtattg ctttggatca tatcgatgaa
     5881 atcattgcga ttatccgcgg gtctaaatca gatgacgaag caaaagcgac gatgatcgaa
     5941 cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt acgccgattg
     6001 accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa actgattgaa
     6061 gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa gaatgaatta
     6121 gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt cggtgaggtc
     6181 ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac attgacaaac
     6241 aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag gggtggacgt
     6301 ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt ttcttgttct
     6361 acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc aaaaggatac
     6421 gaaattccag aatacgggcg cacagccaaa ggaataccaa ttatcaattt actaggtatc
     6481 gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga agaaggccac
     6541 tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct
     6601 aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat
     6661 gtgctactga cagatggtca atctaatatc attattggaa cacataacgg ctactctgtc
     6721 actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt tagaggaata
     6781 cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaga tcaagaagta
     6841 ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa
     6901 ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct
     6961 ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt
     7021 atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg
     7081 atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca
     7141 gaacaaacag ttgaagaaca aggatcagaa gaatagccat caagactaat tgtgtaagct
     7201 agcaagaatg accgattaaa aaatctggtt attcctgctg gcttttttga ttagttgaat
     7261 ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat
     7321 aatcagagct tgtgagtagt ctaattaatt agataactct ccttgctctt aggaacctaa
     7381 gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg aaatcatgta
     7441 cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc
     7501 aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa aacgccgttt
     7561 cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag tatcttctcc
     7621 atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg acgatatcat
     7681 tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac aatagaaaac
     7741 gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt ctgacgaaag
     7801 atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact ttggctgtga
     7861 atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac tgtgtgattt
     7921 ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt ttaggtgtta
     7981 ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta tacgtgacag
     8041 aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa aatcgccaac
     8101 aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga aacaataact
     8161 ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat acttctgacc
     8221 cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc taaacgaatt
     8281 tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta
     8341 gactacattg ctgctaacca tattgaatat atcgattata aagatatcga attactaaaa
     8401 agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac aggcgctaaa
     8461 aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc
     8521 gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat cagctattca
     8581 attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg acgaaagtta
     8641 tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa acaatcattt
     8701 atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg tagtcttaat
     8761 tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat ggggtgttcg
     8821 ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc
     8881 ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg taggagtgat
     8941 tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat tgaaaatcaa
     9001 agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg caaaagaaaa
     9061 aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg atacagtaaa
     9121 gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc atcagacgaa
     9181 cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca ttgataaatt
     9241 ggatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt ctgactggat
     9301 gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct tcgtagcaca
     9361 gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc gctttcgagc
     9421 agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt atggctcaag
     9481 aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt tagtccgtgg
     9541 aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt attatggggg
     9601 gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt
     9661 gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt ttccggatat
     9721 ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc
     9781 ttacatcgtt atcaacgaga acgagatcat tccagatgta gaacggtgtc ttgaagaaat
     9841 ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca tagagctgaa
     9901 aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat cgatctctca
     9961 gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc gtggggaaga
    10021 gtttccaagt aaacctctgc tgacttatat cgaatcttca gcttcttcag cttcagagct
    10081 agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc gttttgaggc
    10141 cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc
    10201 acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat ccttagtaca
    10261 atatttattg agctcggatt tagcgacata cttggagatc agcaatttga ttgctggaag
    10321 cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag agtataatag
    10381 tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca atgcagcttt
    10441 cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta ccggcacgat
    10501 caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa acgcagctac
    10561 tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg caatcaaaga
    10621 aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt tttacaagat
    10681 gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta tgcacaaaat
    10741 tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca
    10801 ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc taaaaagtta
    10861 aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc aggggaagac
    10921 agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa caaacaatac
    10981 aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc actaggctac
    11041 acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta
    11101 caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga aggaaaaaag
    11161 acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga
    11221 tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag aaaacgagta
    11281 taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact tttctctcat
    11341 tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga agtgtggcag
    11401 gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc tgtttttctt
    11461 gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt ttatcgtcgt
    11521 ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga agcgattgac
    11581 gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc cggtggttta
    11641 agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt ctactatgcg
    11701 aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac aggaatcgtt
    11761 acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc cgaacgcagt
    11821 atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc tgaagtactg
    11881 aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat cacaggtcta
    11941 ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga agaactaatc
    12001 attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat
    12061 gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg ggcagaatct
    12121 cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt acgtacaggt
    12181 cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct ttctcgagca
    12241 aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc gaaatgtcgg
    12301 aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt gcagttgatt
    12361 gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa
    12421 aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc tcgtggagta
    12481 gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg atcaattgaa
    12541 caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag agaaggcgga
    12601 gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat catcgagaag
    12661 aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata caataagttt
    12721 tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg agcaatcccg
    12781 agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta cttaatttaa
    12841 acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc cgaataaacg
    12901 gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg aggaactatt
    12961 ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct cttttcctta
    13021 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa catgagaagc
    13081 agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca acctctttct
    13141 taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga aaaatatgag
    13201 aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
    13261 gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc acaaaaattt
    13321 gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc tgttacaacc
    13381 tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt ttataaaata
    13441 aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac aatggttcag
    13501 gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat
    13561 ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct gaagtgattg
    13621 cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt gtgacttata
    13681 aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg atcggaaacg
    13741 gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat gaaaaaggtg
    13801 tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct tatcatatca
    13861 aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca acgattaaag
    13921 gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt gcagacttat
    13981 tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat
    14041 ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa gaatattatg
    14101 aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg aatgatgctt
    14161 tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc
    14221 agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta acaatcggaa
    14281 gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac
    14341 gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa caaatccgcg
    14401 aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt
    14461 ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct ttaaactcga
    14521 tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg
    14581 aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg
    14641 aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca gaactgccag
    14701 aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt atctcaacat
    14761 tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg gcgcaaatct
    14821 aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga agtgtttatc
    14881 tatacgataa taataacttg aacactgttt attcagaata agggactgtg acaaaaaccg
    14941 tgtcacagtc cctttttccg aa
//
LOCUS       DARDHU010000068        14815 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000068 DARDHU010000000
VERSION     DARDHU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.068"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(296..1024)
                     /locus_tag="SDD03_001982"
     CDS             complement(296..1024)
                     /locus_tag="SDD03_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG4304421.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="SDD03_001983"
     CDS             complement(1002..2576)
                     /locus_tag="SDD03_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4304422.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="SDD03_001984"
     CDS             2805..3743
                     /locus_tag="SDD03_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4304423.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="SDD03_001985"
     CDS             3755..4666
                     /locus_tag="SDD03_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4304424.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="SDD03_001986"
     CDS             4690..6162
                     /locus_tag="SDD03_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4304425.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="SDD03_001987"
     CDS             6213..6824
                     /locus_tag="SDD03_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4304426.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="SDD03_001988"
     CDS             6854..8767
                     /locus_tag="SDD03_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG4304427.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="SDD03_001989"
     CDS             8895..11876
                     /locus_tag="SDD03_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG4304428.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="SDD03_001990"
     CDS             11873..13534
                     /locus_tag="SDD03_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG4304429.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="SDD03_001991"
     CDS             13574..14506
                     /locus_tag="SDD03_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG4304430.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DARDHU010000069        14482 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000069 DARDHU010000000
VERSION     DARDHU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.069"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(287..649)
                     /locus_tag="SDD03_001992"
     CDS             complement(287..649)
                     /locus_tag="SDD03_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304431.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="SDD03_001993"
     CDS             complement(723..1103)
                     /locus_tag="SDD03_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG4304432.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="SDD03_001994"
     CDS             1167..2360
                     /locus_tag="SDD03_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4304433.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="SDD03_001995"
     CDS             complement(2437..3021)
                     /locus_tag="SDD03_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4304434.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="SDD03_001996"
     CDS             3222..4220
                     /locus_tag="SDD03_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4304435.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="SDD03_001997"
     CDS             4332..4841
                     /locus_tag="SDD03_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG4304436.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="SDD03_001998"
     CDS             complement(4871..5530)
                     /locus_tag="SDD03_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG4304437.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="SDD03_001999"
     CDS             complement(5532..6296)
                     /locus_tag="SDD03_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG4304438.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="SDD03_002000"
     CDS             complement(6304..6981)
                     /locus_tag="SDD03_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG4304439.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="SDD03_002001"
     CDS             complement(7018..8403)
                     /locus_tag="SDD03_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4304440.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="SDD03_002002"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SDD03_002002"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="SDD03_002003"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="SDD03_002003"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG4304441.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="SDD03_002004"
     CDS             9342..10199
                     /locus_tag="SDD03_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4304442.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="SDD03_002005"
     CDS             complement(10280..10615)
                     /locus_tag="SDD03_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304443.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="SDD03_002006"
     CDS             complement(10682..12052)
                     /locus_tag="SDD03_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG4304444.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="SDD03_002007"
     CDS             complement(12130..12666)
                     /locus_tag="SDD03_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4304445.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="SDD03_002008"
     CDS             12975..14456
                     /locus_tag="SDD03_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4304446.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2883 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta tt
//
LOCUS       DARDHU010000070        14018 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000070 DARDHU010000000
VERSION     DARDHU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.070"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            276..2441
                     /gene="acm"
                     /locus_tag="SDD03_002009"
     CDS             276..2441
                     /gene="acm"
                     /locus_tag="SDD03_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG4304447.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2713..3198)
                     /locus_tag="SDD03_002010"
     CDS             complement(2713..3198)
                     /locus_tag="SDD03_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG4304448.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3288..3633
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3678..4319
                     /locus_tag="SDD03_002011"
     CDS             3678..4319
                     /locus_tag="SDD03_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4304449.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4338..4973
                     /locus_tag="SDD03_002012"
     CDS             4338..4973
                     /locus_tag="SDD03_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4304450.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4991..5806
                     /locus_tag="SDD03_002013"
     CDS             4991..5806
                     /locus_tag="SDD03_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4304451.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6125..7561
                     /locus_tag="SDD03_002014"
     CDS             6125..7561
                     /locus_tag="SDD03_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4304452.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7750..10197
                     /locus_tag="SDD03_002015"
     CDS             7750..10197
                     /locus_tag="SDD03_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG4304453.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10427..11494
                     /gene="rlmN"
                     /locus_tag="SDD03_002016"
     CDS             10427..11494
                     /gene="rlmN"
                     /locus_tag="SDD03_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG4304454.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11687..12790)
                     /locus_tag="SDD03_002017"
     CDS             complement(11687..12790)
                     /locus_tag="SDD03_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4304455.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13343..13891
                     /gene="aac(6')"
                     /locus_tag="SDD03_002018"
     CDS             13343..13891
                     /gene="aac(6')"
                     /locus_tag="SDD03_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG4304456.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4821 a         2309 c         2959 g         3929 t
ORIGIN      
        1 gttgcatttt tgtaagttaa aaattggaaa tatagattga aaaaatgaaa gcaataagta
       61 aaataaaatg aatatatgcg aaaaaataat ttacattagg aaaaaccttg taaaaacagt
      121 tttttggcat atttattttt tataaatatt tgtaaaataa ctatcaattt tttagttaaa
      181 agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg atatagtagg
      241 ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga aatattattt
      301 tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat gtctatgcgg
      361 atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca acgaatatta
      421 cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa aatatccaac
      481 caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt gaaggattcc
      541 aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg gtcagaaaag
      601 attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta gaaggttggg
      661 ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat acgggaccat
      721 tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt
      781 cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat actaagcaca
      841 ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca gttaaaatca
      901 gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt aatcaaatac
      961 acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt ttacaagatt
     1021 tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata actataccaa
     1081 aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata gaaaacccag
     1141 aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat aaacctgctg
     1201 ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt ggagtcaacg
     1261 gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag attggtatcc
     1321 caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga cagtctagta
     1381 tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa gtaggagact
     1441 atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta agttcaaatg
     1501 aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct atttataaca
     1561 agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc actccaagac
     1621 cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg
     1681 cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat atccaacagt
     1741 atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa aacggaaatg
     1801 attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca aatgagaata
     1861 aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac caagacggca
     1921 aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc caacacaaag
     1981 aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa tacagagacg
     2041 ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt acgacgattg
     2101 acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct ataccaggta
     2161 gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa attagaaatc
     2221 tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa caaagaagca
     2281 attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa agcattcttc
     2341 ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg atagtaacta
     2401 tctgtgctgg gatattctta caaaaatata aaaaagttta gttagaatat aatatttttt
     2461 gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa aaagaagcat
     2521 ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta actatggaag
     2581 aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag
     2641 aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa
     2701 actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt
     2761 ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac
     2821 ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg
     2881 catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc
     2941 ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc
     3001 gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa
     3061 tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc
     3121 aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg
     3181 ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag
     3241 agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga
     3301 aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag
     3361 ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg
     3421 agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga
     3481 gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact
     3541 ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa
     3601 cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt
     3661 taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag
     3721 cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg
     3781 ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg
     3841 tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc
     3901 cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact
     3961 atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat
     4021 atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc
     4081 cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag
     4141 attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga
     4201 tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga
     4261 ctggtattct aacaatcata atgaaacaag ttgaaggaaa attaaattat taccgctaac
     4321 tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa
     4381 aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc
     4441 cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca
     4501 gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac
     4561 aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa
     4621 acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag
     4681 cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc
     4741 tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg
     4801 tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc
     4861 gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag
     4921 tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga
     4981 gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt
     5041 tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa
     5101 aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg
     5161 gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac
     5221 ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat
     5281 cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac
     5341 aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa
     5401 ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt
     5461 atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta
     5521 tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat
     5581 ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat
     5641 aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac
     5701 attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa
     5761 aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg
     5821 tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct
     5881 tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag
     5941 tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt
     6001 cttactactc gaaactaagc gactttttca caacctcctt tacataattt tattttgttg
     6061 tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag
     6121 atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc
     6181 tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga
     6241 tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga
     6301 ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta
     6361 ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc
     6421 tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat
     6481 gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga
     6541 atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg
     6601 cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta
     6661 tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca
     6721 tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg
     6781 aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa
     6841 tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat
     6901 gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac
     6961 gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa
     7021 ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat
     7081 gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa
     7141 agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt
     7201 aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa
     7261 tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat
     7321 caattactta caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt
     7381 cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa
     7441 gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata
     7501 taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta
     7561 gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa
     7621 tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc
     7681 tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag
     7741 ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg
     7801 aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt
     7861 ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc
     7921 tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga
     7981 ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc
     8041 ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc
     8101 catactgggg aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa
     8161 caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt
     8221 acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga
     8281 ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt
     8341 ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg
     8401 gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca
     8461 aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa
     8521 agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt
     8581 tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta
     8641 ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat
     8701 gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta
     8761 ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa
     8821 attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag
     8881 gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg
     8941 gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa
     9001 gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat
     9061 ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat
     9121 aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt
     9181 tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt
     9241 aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg
     9301 atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg
     9361 gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa
     9421 gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt
     9481 gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg
     9541 atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt
     9601 attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt
     9661 ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt
     9721 gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc
     9781 ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa
     9841 ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa
     9901 gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca
     9961 gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg
    10021 gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta
    10081 gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa
    10141 gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac
    10201 ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca
    10261 aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt
    10321 ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat
    10381 catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc
    10441 catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa
    10501 attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga
    10561 aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc
    10621 tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact
    10681 ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg
    10741 tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa
    10801 aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta
    10861 ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga
    10921 gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg
    10981 attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa
    11041 ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa
    11101 tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga
    11161 agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt
    11221 gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa
    11281 gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata
    11341 ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt
    11401 aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg
    11461 aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta
    11521 ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt
    11581 ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg
    11641 tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct
    11701 tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt
    11761 taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt
    11821 tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg
    11881 gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca
    11941 tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt
    12001 tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt
    12061 taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg
    12121 catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac
    12181 gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc
    12241 cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata
    12301 agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt
    12361 ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag
    12421 tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt
    12481 caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg
    12541 ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc
    12601 cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt
    12661 ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga
    12721 cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac
    12781 tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga
    12841 tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa
    12901 taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa
    12961 aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt
    13021 gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt
    13081 atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca
    13141 aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt
    13201 ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag agatactgat
    13261 tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa
    13321 gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt
    13381 gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc
    13441 ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca
    13501 agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt
    13561 gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa
    13621 ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga
    13681 tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt
    13741 ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa
    13801 aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa
    13861 aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag
    13921 cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg aaattccaac
    13981 ttatttccga agaagctact tttggaacac cgtgtacc
//
LOCUS       DARDHU010000071        13810 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000071 DARDHU010000000
VERSION     DARDHU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.071"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SDD03_002019"
     CDS             complement(221..1423)
                     /locus_tag="SDD03_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4304457.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SDD03_002020"
     CDS             complement(1426..1620)
                     /locus_tag="SDD03_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG4304458.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SDD03_002021"
     CDS             complement(1667..2515)
                     /locus_tag="SDD03_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG4304459.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SDD03_002022"
     CDS             complement(2720..3070)
                     /locus_tag="SDD03_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304460.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SDD03_002023"
     CDS             3665..4111
                     /locus_tag="SDD03_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG4304461.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SDD03_002024"
     CDS             4735..5727
                     /locus_tag="SDD03_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4304462.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SDD03_002025"
     CDS             5743..6627
                     /locus_tag="SDD03_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4304463.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SDD03_002026"
     CDS             6676..8283
                     /locus_tag="SDD03_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4304464.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SDD03_002027"
     CDS             8284..9768
                     /locus_tag="SDD03_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG4304465.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SDD03_002028"
     CDS             9945..10937
                     /locus_tag="SDD03_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4304466.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SDD03_002029"
     CDS             10989..11858
                     /locus_tag="SDD03_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4304467.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SDD03_002030"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SDD03_002030"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG4304468.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DARDHU010000072        13404 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000072 DARDHU010000000
VERSION     DARDHU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.072"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            79..1125
                     /locus_tag="SDD03_002031"
     CDS             79..1125
                     /locus_tag="SDD03_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG4304469.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="SDD03_002032"
     CDS             1474..2106
                     /locus_tag="SDD03_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG4304470.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="SDD03_002033"
     CDS             2694..4133
                     /locus_tag="SDD03_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4304471.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="SDD03_002034"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="SDD03_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="SDD03_002035"
     CDS             4895..5110
                     /locus_tag="SDD03_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304472.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="SDD03_002036"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="SDD03_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="SDD03_002037"
     CDS             complement(5770..6681)
                     /locus_tag="SDD03_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4304473.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="SDD03_002038"
     CDS             complement(6700..7704)
                     /locus_tag="SDD03_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG4304474.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="SDD03_002039"
     CDS             complement(7701..9824)
                     /locus_tag="SDD03_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4304475.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="SDD03_002040"
     CDS             complement(9829..12276)
                     /locus_tag="SDD03_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4304476.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="SDD03_002041"
     CDS             complement(12263..12652)
                     /locus_tag="SDD03_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4304477.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="SDD03_002042"
     CDS             complement(12712..13215)
                     /locus_tag="SDD03_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4304478.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13389)
                     /locus_tag="SDD03_002043"
     CDS             complement(13228..13389)
                     /locus_tag="SDD03_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304479.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4099 a         2642 c         2454 g         4209 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DARDHU010000073        13343 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000073 DARDHU010000000
VERSION     DARDHU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.073"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            15..161
                     /locus_tag="SDD03_002044"
     CDS             15..161
                     /locus_tag="SDD03_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG4304480.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(223..2034)
                     /locus_tag="SDD03_002045"
     CDS             complement(223..2034)
                     /locus_tag="SDD03_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG4304481.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2031..2396)
                     /locus_tag="SDD03_002046"
     CDS             complement(2031..2396)
                     /locus_tag="SDD03_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG4304482.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2559..2954)
                     /locus_tag="SDD03_002047"
     CDS             complement(2559..2954)
                     /locus_tag="SDD03_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4304483.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2972..3934)
                     /locus_tag="SDD03_002048"
     CDS             complement(2972..3934)
                     /locus_tag="SDD03_002048"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG4304484.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3934..4146)
                     /locus_tag="SDD03_002049"
     CDS             complement(3934..4146)
                     /locus_tag="SDD03_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG4304485.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4167..4865)
                     /locus_tag="SDD03_002050"
     CDS             complement(4167..4865)
                     /locus_tag="SDD03_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG4304486.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4885..5427)
                     /locus_tag="SDD03_002051"
     CDS             complement(4885..5427)
                     /locus_tag="SDD03_002051"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG4304487.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5559..6563)
                     /locus_tag="SDD03_002052"
     CDS             complement(5559..6563)
                     /locus_tag="SDD03_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG4304488.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6560..7549)
                     /locus_tag="SDD03_002053"
     CDS             complement(6560..7549)
                     /locus_tag="SDD03_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG4304489.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7546..8352)
                     /locus_tag="SDD03_002054"
     CDS             complement(7546..8352)
                     /locus_tag="SDD03_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4304490.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8518..9474
                     /locus_tag="SDD03_002055"
     CDS             8518..9474
                     /locus_tag="SDD03_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4304491.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9551..10069)
                     /locus_tag="SDD03_002056"
     CDS             complement(9551..10069)
                     /locus_tag="SDD03_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4304492.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10157..10306)
                     /gene="rpmG"
                     /locus_tag="SDD03_002057"
     CDS             complement(10157..10306)
                     /gene="rpmG"
                     /locus_tag="SDD03_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4304493.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10534..10980
                     /locus_tag="SDD03_002058"
     CDS             10534..10980
                     /locus_tag="SDD03_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG4304494.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11175..13070)
                     /gene="asnB"
                     /locus_tag="SDD03_002059"
     CDS             complement(11175..13070)
                     /gene="asnB"
                     /locus_tag="SDD03_002059"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG4304495.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4152 a         2831 c         2276 g         4084 t
ORIGIN      
        1 aatttgtatc tggaatatta gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg
       61 ttgcttctgt gaaaaaaaca gtgatcgatc caattgacga aaaagaagca atgatcgaag
      121 aaaaccgtaa aaaagcaatg cgtaaacgcg tttctcgtta ataattattt aaaaatacgc
      181 atgaagactt tctaaaaaac agataagtca tcatgcgtat ttttatttgt cagtcaatat
      241 aagttgtgct attttatctg cctgtctcag attccatgga tctgtataag gtctagctac
      301 tgctgcagtt agccaatcca caccttcaac aggtattcca tagcaataaa agtcagactg
      361 gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta tagcttctga
      421 atgataactt cctgtattat ctgaaaatga aaaagggcga acaagtttgt tttccttcaa
      481 attttttatg acaggattac ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat
      541 caaatagcga ctgctgattt ctttatcttg tagagaaata acatagcggt tgtgcttttg
      601 ctgaatatca aatggcggtt cgatcaaatg aaaaataccc gcatctatca gtgctgctaa
      661 ttctttcgtc cgaatgattg gcggaccgat cgttaaaaag ctatttagat gagtaaacca
      721 tccccatagt atttctttgt attccttagc agaaaaaacg tgccattcaa tagcctgccg
      781 gattggatca cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt
      841 gcctttttcc gcttctttta tctgcccttc aataaatatt cggctagctt ctgaaaaggg
      901 ctcattctga taggcaagag gagtttctaa atacgaccaa tcccatctat aagggattag
      961 tgctggatgc tttccgagac actcctctga agagagggag agaaaatcat gctcaaaatt
     1021 tttctttgga atcggcaagt gatattcttc gatgattttc ttgtaataaa acaactcaac
     1081 gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa aatcccgact gatgccattg
     1141 ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt
     1201 tcttcctcga gggtaataag ggagccccct tcttgatcca ctgtatacaa tcggttcctt
     1261 gcctgatgga tgatagacca gttttccctt ttcgttctcg aagcgccctc ctcttgatac
     1321 tgtaaacaat ccgacataat caaaaaaact taagcctaga cctcttaaaa agacattttc
     1381 tttaggcggt atcttcgaaa gatctacatc tgcaggatta gaaggaagct gatacagtaa
     1441 aacatttttc tttgcgtagt ctacccacac tctttcttct tctgtctcat cattttccca
     1501 gtgaccgctt gctaaaacaa gctgatcgat agaatagctt tgctgatctg tctgtaaaag
     1561 ataaccatct tttcttttgc ttactttttt tactaaagaa taaacaaact gaatagaagc
     1621 tggaaattct tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg
     1681 agtagattgc tgatcttttt ccagacgatt gatttcttct ataattagtt gcttattttt
     1741 ggttaccgtc tgttcgatat aatctgtagc aggtccttta ctccattgaa acaagttggg
     1801 tccctgtgtt accaatcctc cactggacaa tgtttcatct gtaaaaagcg tcacttgttg
     1861 agaaacagaa ttcattaata actccggtgg ctgattcaac cgccaaactc gacctccagg
     1921 accattggga tcgaaaagaa aaagttcaag agaatgtctg tcctgattat tatggagaat
     1981 cctttctgct acacttaatc ctctaggtcc ggcaccaatg atccctattt tcatttgcat
     2041 gcctccattt cgataaattt tttcaataaa acagcatggt tctgatgttc attttttgca
     2101 ccataaacaa aagtaatgac tggatgttct ttcataagtt gactgagttt aaaaatagct
     2161 tgcttctttt cttcttcgaa aatcaattct tgctggtatt tctttgcaaa tgaagcaaat
     2221 cgttctggta catggtcaaa agttttgcgt gcttcattac taggtgcaat ttctttcaac
     2281 cacagatcta actgcgcttt ttcttttgaa attcctcgtg gccagaatct atccacgagt
     2341 attctatagc catcctcttt ttcagcatca aggtaaacac gttttaattt gatcatagtt
     2401 gtttccttct ttctaccttt tcgcttagct tatatatgta gataatagca caaaataaaa
     2461 aaaatgaacg gcacaactat ccattgcgcc gttcatgtga tcttggtcaa aatctaatga
     2521 ttgattcggt ttttaaagct gtcatacaat catctgtttt atactttttt acttttgatt
     2581 tttccagacc atccgtcata tccaccctta agtaaataaa tctcttgata acctgcttta
     2641 cgtaatttat ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat atagacaggc
     2701 tggtcttttc gaatagatcc taatgaatct ttcaaaacag tgtaagggat gtttcttgca
     2761 cctaaaatat gcccagcatc aaacacatct ttttcgcgaa catcgattac ttgtgctttt
     2821 ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt ggccattact
     2881 cgtagataga cttcccaaag gatgattgca agcacaatca atagcaaaat gatattaatt
     2941 acccataaaa ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct aatgaggcag
     3001 caccaatgac accagcttca ttaccaagct ctgctaattt gatttttgtt gattctctta
     3061 cttgcgggaa agtaaattct ttaaaatatt tctctacacg gctacgtaaa aattctcctg
     3121 ctgccgatac tcctccacct aacacgatac ttgacggatt taatgtattc cccaagtttc
     3181 cgcaagctaa acctaagtag aagcatacgc gatcaacaac catcaatgca aacggatcgt
     3241 cttcttgagc catttcaaag atatctttac ttgttacttc ttctccgttg tctagcattg
     3301 ctttcagttt tgaatcccct gcgtattctt ctgccaagaa acgacctagg cgtaccacac
     3361 ctgtagcgct agatacagtt tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt
     3421 caggatccac agtgatatgt ccgatttctc ctccagctcc gctcacgccg tgaatcaaat
     3481 ttccgccagc aacgatacct ccgccaacac ctgttcctag agtaacgaat acaacgtctg
     3541 gatcgttttc tccagcacct ttccatcgtt cacctaaagc agcaacattc gcgtcattat
     3601 cgatagcgaa tttgattcct gttcctgcct cgatcagttt tttcacaaat tgaacttctg
     3661 tccagtttaa gttatacgca ccaataaccg ttccagcttc gctgtccacg cttcctgggg
     3721 tacccatgcc gattccgata aagtcttcgg ctttcatatt atataaattc aaacgatgat
     3781 tgattgattc aatgatttca ggaacgatat gcttaccatc atccaaaata ttggtatcga
     3841 tgctccattt ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt gctgttgttc
     3901 cacctaaatc aatcccaata atcttcttgt ccattacaaa tttcctttct ttcggctttc
     3961 ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat catgatcaat
     4021 caaacggcta tcatacagat tacgcaattc gatagtcatt aattcaatgt catagatgcg
     4081 tgcacccaca tagataaaga tgccaaattt tttgaacagc tgctgtacat catataagct
     4141 ctccatcttt aagcacttcc ttcatattat acaagtaaac catatttttt taaacccagt
     4201 aaaatacata ttacaaaaag aaatacaaaa actagtgtag aaagaatcct ttggtgtatc
     4261 gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa tccgccaatt
     4321 agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa aacaaatgtc
     4381 aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt atgtttaaaa
     4441 tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact tgcaccacca
     4501 gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat cccgctgaac
     4561 atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg ccccataaaa
     4621 tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa gattggtgtc
     4681 actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc ggcataataa
     4741 gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg tatccctaaa
     4801 aaagcatagg tccataacgg tcgtcttaac catgcacgca attttatttg ctgttgataa
     4861 ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct tctgatcaaa
     4921 gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac atgttttttt
     4981 atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc gataaccggc
     5041 tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca aatagagctt
     5101 ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact gttcatccat
     5161 ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt tcttttgttc
     5221 tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg ataaagtcgt
     5281 ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat agattcgctg
     5341 ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga ttcctttccg
     5401 gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat aaaatggtat
     5461 aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag aataaaaaga
     5521 agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag cgcgtgtttt
     5581 tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat caaaattcct
     5641 gtaggtactt ccccacttgg tagaatcact ctagcaacca tatctgcact aagcagaaaa
     5701 atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg tacgactagc
     5761 ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac cgctacacta
     5821 ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc cacgttaagg
     5881 cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg tgcataaagt
     5941 cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata aggccagctc
     6001 gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc cttagataat
     6061 tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat accggacaat
     6121 aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata gacacagcaa
     6181 gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag cgaatagtgc
     6241 gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat gcccaatata
     6301 ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga aacgccaaga
     6361 caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct aacaatcagc
     6421 gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa
     6481 ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag caagaaatag
     6541 aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt aaaaacggaa
     6601 cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg atcatcctcg
     6661 ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca ggtagaacat
     6721 aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc ccaataaatg
     6781 aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga cagaaaacaa
     6841 tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa
     6901 tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata tataaaggaa
     6961 acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac caaaaagcaa
     7021 gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta aaaaaagaac
     7081 taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc gaaaactgca
     7141 aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt agaaacacag
     7201 aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac gaagcacccg
     7261 cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcacccct tgcatcaatg
     7321 caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa
     7381 tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac gcctgccaaa
     7441 aatctgcttt ttccacagtc gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa
     7501 gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata attgatcctc
     7561 ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc ttggaagcgt
     7621 gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga tcacttcttc
     7681 aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat ctgaaaaacg
     7741 tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca tttgattgat
     7801 ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc
     7861 atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga cacgctgggt
     7921 ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac ctgtagcatc
     7981 caacgcccac tcaattgctt catgatcttg cgtgtccatg ccactgaaca gtttttgata
     8041 agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt gttcactagt
     8101 ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag agatatcaga
     8161 gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac ttttcaataa
     8221 agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt caggaatcgt
     8281 aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt cagctgctct
     8341 taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg acgctgtttc
     8401 aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg acagcgttcg
     8461 aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag gaaagaaatg
     8521 aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg agtactgagc
     8581 gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac gacttcatcc
     8641 aatagtactc atacagtcac agatacactt ggacataaag tagagattcc aaacgcgccc
     8701 aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga aaaacctatc
     8761 gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga tttaaaggat
     8821 gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga gcctgatttg
     8881 ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc
     8941 gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca actaaaagat
     9001 gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa
     9061 tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac agctgcagtg
     9121 ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag tggaagaatc
     9181 gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc caaaaaagcc
     9241 acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc tgactacatc
     9301 atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg gaagaatcta
     9361 aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg gctatataac
     9421 ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa ttgattttaa
     9481 ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta acaaactaga
     9541 cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc catttttgaa
     9601 aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct ataagggcga
     9661 agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg
     9721 ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta ccacccgaaa
     9781 catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc gaatgcatta
     9841 cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg atatggcgat
     9901 aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact aaaattcgat
     9961 ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt aacaaaccat
    10021 ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata ttatccgctc
    10081 cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca gcaaggaatt
    10141 gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc
    10201 aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt gttagatagt
    10261 tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt ttccctccca
    10321 tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat gattttttct
    10381 agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata aatacaacgg
    10441 ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac ttgtttatat
    10501 agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa ggactttcct
    10561 gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat cgcaaatgca
    10621 ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa
    10681 aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca
    10741 agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga ttcagatgtg
    10801 atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc
    10861 aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa
    10921 gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat cgattattga
    10981 acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg
    11041 gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg aagagctgtt
    11101 ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc ttttttattg
    11161 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
    11221 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
    11281 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
    11341 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
    11401 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
    11461 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataaa
    11521 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
    11581 acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc ttttggcatc
    11641 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
    11701 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgttaac
    11761 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
    11821 ggagcaagtg attcatataa atggatccgt ccatggaagt ttttcatttt tccgatttta
    11881 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
    11941 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
    12001 ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa
    12061 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
    12121 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa tagttcggtt
    12181 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
    12241 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
    12301 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
    12361 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
    12421 tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg caaacgatag
    12481 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
    12541 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
    12601 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
    12661 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
    12721 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
    12781 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
    12841 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
    12901 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
    12961 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
    13021 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
    13081 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
    13141 cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg
    13201 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctacc
    13261 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa
    13321 aagacacgac ctaaagatcg tgt
//
LOCUS       DARDHU010000074        13111 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000074 DARDHU010000000
VERSION     DARDHU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.074"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            46..915
                     /locus_tag="SDD03_002060"
     CDS             46..915
                     /locus_tag="SDD03_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG4304496.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1124..2149
                     /locus_tag="SDD03_002061"
     CDS             1124..2149
                     /locus_tag="SDD03_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG4304497.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2188..3138
                     /gene="manA"
                     /locus_tag="SDD03_002062"
     CDS             2188..3138
                     /gene="manA"
                     /locus_tag="SDD03_002062"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG4304498.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3232..4935
                     /locus_tag="SDD03_002063"
     CDS             3232..4935
                     /locus_tag="SDD03_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4304499.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            4937..6970
                     /locus_tag="SDD03_002064"
     CDS             4937..6970
                     /locus_tag="SDD03_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4304500.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            6981..7421
                     /locus_tag="SDD03_002065"
     CDS             6981..7421
                     /locus_tag="SDD03_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4304501.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7621..8748)
                     /locus_tag="SDD03_002066"
     CDS             complement(7621..8748)
                     /locus_tag="SDD03_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4304502.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8751..9428)
                     /locus_tag="SDD03_002067"
     CDS             complement(8751..9428)
                     /locus_tag="SDD03_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4304503.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9692..11458
                     /locus_tag="SDD03_002068"
     CDS             9692..11458
                     /locus_tag="SDD03_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG4304504.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11473..11910
                     /locus_tag="SDD03_002069"
     CDS             11473..11910
                     /locus_tag="SDD03_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4304505.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            11923..13074
                     /locus_tag="SDD03_002070"
     CDS             11923..13074
                     /locus_tag="SDD03_002070"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG4304506.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4446 a         2281 c         2720 g         3664 t
ORIGIN      
        1 aatccatcgt taaaataaag cttgacgtag aggggagcaa gaaatatgaa agattttcaa
       61 ttgggggctg ccacctattt aaaaagtgta gccattcggg tgaaagaccg agataaaatg
      121 attgattttt acaagaacgt tgttggcttt gatttgaaaa gagaagaaaa cgaattagcc
      181 attttaggaa cagaagaagc tggaagtgaa atgttgctgc tggaagaaag tcctagagcg
      241 aatgatcaca tgggggtgat caaaaagctg aaccgttttt cattgattat ccctagcaca
      301 gaagaaatgg cagatatcct ttgtcgtatc cgtaaaatga attatccaat caaagaagct
      361 ttagaagatc atggacgtat ggggattttg ctcgtagacc cagaagagaa tgaattggaa
      421 atttaccatg aagagaataa gaacgaccaa agagaaattc cagaaacaat ggatcaagaa
      481 gcattattaa gaaaatctga agaaaaattc agtaaactat caacaggttc ttattttgaa
      541 aaaatccatt taaatgttac taacttaaca aaagaacgcc aatttcttaa tcatgtactt
      601 ggtttgaagg tacataatga agaagagggc atccatgtat tgaatgaagg agatttccat
      661 gttggtttga cagaagctag cggaggcgca attgatttgc ctacagatga agtgattgga
      721 cttgattttc tgaaattcat gatcgatact gaaagcatgg aaggactgat tgctcacttg
      781 cagcaactat cagttgattt cttcttagac aaaaagcaca cagtactcac aatctatgat
      841 gctataggaa tcgaatggtg gttcgtcagt gagccaaaag aaaagaataa aactgacatt
      901 cgatgcgacg cttaactttt tctttcttaa cgattgtcgc atgtctcgtc attttatgaa
      961 cggtcatact atcgcttatt ctagagatag aaagagaaaa tagtataccg ggggaaactg
     1021 cgcggttttt cgcgcagttt ttttgtgtaa aaaaggttga ttcctcatag attaagcttt
     1081 aagcttggac tgtaagaatc tgatagaaaa gcaggagaaa aagatgtatc cacacacgaa
     1141 acagcttatt ttgaaaaaga tggaagaagg attttatcca ggtgtagcag tcgccttcat
     1201 aaaagaaaat caagtagaaa catttacagc cggctttgcc caagtcgttc ctgaaaagca
     1261 gctgatgaca gaagatctgt tgtttgatgc agcctcactg acgaaagtcg ttgcaaccac
     1321 ttcattagtt ttacgtctat tagaagatgg aaagatagaa ctcgatcaac cgcttcataa
     1381 atatcttcct gaatttgaaa atcagacaat cactttgcgt catttactga cacatacttc
     1441 tgatatcaaa acatggatac caaacagaga taagctatct caggaagaat tgatcgccgc
     1501 ttatttgaag ctagaaccag gtgagcaact aggaaaagaa gtaaaatata cagatgccgg
     1561 tacgattctt cttgggttga tgctagagga aatttaccaa gaaactgcag tggaaatctt
     1621 tcaagaacaa gtattggaac cattaggaat gatgaacagc ttatttcttc cttttccttc
     1681 caaaaaaatc gttcccacag aacaactgcc ttcgggtgag gttttgcgtg gacaaactca
     1741 tgatccgaaa gcaagagttt tggctgaaca tgcaggtaac gcgggtctct ttattgattt
     1801 gaaagacagc attacttttg tccaaatgta tttgaacaag ggaaaaatcc gtaatggtcg
     1861 ttttttagaa gaagagacga ttgattcttt actgcaagat caaacaccga aaaaaaatgg
     1921 ctatcgttct ttaggatggg acttaaaaaa ggatgtagaa gacggtcatg cgattctttt
     1981 tcatactggt tatacaggta cgtttttctt gattgatata aaaacacaag aagcgttgat
     2041 atttttgtca aatcgcgtac atcctgtcga tcatcgtgcg gagtatctca tatgtcgaga
     2101 tgaaattgtt gaaaactttt tgaaagagaa agcggtatta ccgaaataat gctataatag
     2161 ctatgaaaaa tgagaggagt ttgaattatg caggaaccta tgtttttaaa accagttttt
     2221 caagaaaaaa tttggggagg aagtcgttta cgttcagtct ttggttttga tatccctaac
     2281 gataaaatcg gagaagattg ggcgatcagt gcgcatcctc atggtgtcag tgtagtagaa
     2341 aatggcgaat tcaaagggaa aagattagat gaactatggt cagaacacaa ggaattgttt
     2401 ggccatcctt cagaaccagt gtttccatta ttgatcaaga tcttagacgc agaagatgat
     2461 ctatctgtac aagtacatcc agatgatgcc tatgggatga aacatgaagg tgaattagga
     2521 aaaactgaat gctggtatat catcgatgct gaacctggtg cagaaatcat ctatggtcat
     2581 catgcaaaga caagagaaga actagctgag atgatcaaag atgggcgctg ggatgacctt
     2641 ttgaagaaag taccagttaa aaaaggtgat ttcttttatg taccaagcgg aacgatccat
     2701 gctattggta aaggaatcat gattttagaa acacagcaaa gttctgacac gacttaccgc
     2761 gtgtatgact acgacagaaa agatgcgcaa ggacaaacaa gagagctgca tatccaacaa
     2821 tcgatcgatg taacaactgt ccctgcgaaa acaccagaat tacaaatcaa agaagtccga
     2881 aaagggaatt catcgatcgt cacttattta gaaactgagt ttttcaatgt atatgagtgg
     2941 gatattaaag gaatcacatc tttcaaaaaa caagcccctt atacattgat gacggtaatc
     3001 gaaggagcag gagaccttgt cgttgacgga aaaacatatc cattagaaaa aggaacaagc
     3061 tgtattattc caaatggcgt ttctgagtgg gcggtccaag gtgaactagc aatcattgcg
     3121 actgaaccag gcaaatagtt cccagatagc tgaaagcagc agacggtttt tcgtttgctg
     3181 ctttttctac ataaaatccg tccatcaata aatagaagta tggtgaaaaa gatggaatac
     3241 aaatggaaag caaacaacag cagtcgcgtg tctatgaaac aactgagaaa agctggacat
     3301 cacttaaatg aaatgatcat gccaaatcta agtctgttta tcgcttgggg aatcttgtca
     3361 ttagcaacaa aaggtgtgtc tggcgatttg cacaccacat tagaagaggt atgccattgg
     3421 atgatccagc tgcttttacc catcttgatc agttattcag gcgggaaatc ggtaggggga
     3481 caaaaagggg cagtcgccgg tgcaattgct tctttagggt tgatcgctcg ttccaatgct
     3541 ccacaaatcg taggagcgat gattatcggg ccgcttgccg gcatcgtata tgaacaattt
     3601 gtttctagat tccacgtgaa attcaaggct ggatatgaaa tgcttgtcag caacctacta
     3661 gtaggactga ctggtagcgt tatttgtata attggaatcg tttttattga accagtttta
     3721 aatagtgttc acttgttgct cgcctccgtt gtgtcttggc tagtcacaat gaatctgtta
     3781 ccacttgtca gtctgatctt agaacctcta aaagtactat ttttcaataa tgcaatcaat
     3841 catggtgtat tgacaccttt aggaatcgaa ttctcacaaa cagtcgggta ctcttcctta
     3901 tttttgatgg aggcaaatcc tggacctggt ttagggatct tactttctat cctttgtttt
     3961 gcagaaaaac aggaaaaagt aaatgcgagt ggagcattga tgatccatgc gattggcggg
     4021 attcacgaaa tctattttcc atttgtcttg ctgcgaccgt atctattttt agctgtaatg
     4081 gctggtgggg caagcggcac gttgatattt caatggttag atacgggatt agctgcacct
     4141 gtatcgcctg ggtcactact cgtgattttt gccaatgcgc cttatccaca actagctggg
     4201 gtaagtatag ggatagcaat cagtacattt gtgaccttca tttgtgcagc gatactattg
     4261 aaattaccaa ataaagaaaa agagaacaaa acggtcaaag aaaggggaga aatcttggaa
     4321 ccatctatca ttcattccgt catttttgct tgcgactcag gtatgggatc aagtgctatg
     4381 ggagccgccc tgctgaaaaa gaaattagca gcagaaaaaa tcgatctttc tgtcggatac
     4441 agctctatct atgaattgaa taaccaagta aatcagctgg ttatcgtgca acatgaacta
     4501 gcgggtcttg caagagaaaa agtacctcaa agtcaaatct atgtactgga tcactttttg
     4561 gaaacagata tcttgctgtc acgaataaaa gaagcgatcc aatttccaaa gcgggaagaa
     4621 aaaaaagaga tagcagatat cacagaacaa aaaaagtcgc ctgctaatat tgtatttttg
     4681 tataaaaaca acagacgcgg ctcacaaact atgggcatga cgattttaag tcaattagca
     4741 aaaaaagcga aaaaacagtt tgttatctca aaagaatcaa tagaaaaaat caaaatccaa
     4801 gaagaaacga tttatatcgc acaaagagaa ctagctgtaa ctgaacagct agaagaaaag
     4861 atccctcggc tagttctagt tgaacattgg gtagctacga atgaatacga aaaactcgtg
     4921 aagggagaaa tataagatgt atttatcgcc cagagaaaaa caattattag cagaatttct
     4981 cagtagcccg tcacctgtat ctatccaaaa aatgatgaat ttattgaagg tcagtaaacg
     5041 gactatttac cgcgaattag ataatcttga agtctcattg aaatcggtaa atgcttcttt
     5101 gaaaaaagca gctagaggct catttgtcat tgaggcatca gaagaagtat tagagcaatt
     5161 aaaagaaaat acagatggga aaagtaatga cttatccact gctaaacgac aacacgcgat
     5221 tttagccgag ttgttactga cgacagagcc gttgagtctt gctcatttca tggaatcata
     5281 tcgcatcagt aataccactt tttatgcaga tatcaaacag ttggaagaaa gtattcgcca
     5341 actgcctttg gaaatcattc ggaaccaggg atacgagatc gaaggaccgg aaaaataccg
     5401 ccggcttctg acagcgaatg tattagaatt agaaatcaat gaatatgaac tttttcatag
     5461 tatttctttc gatgcatcac tgaattattt ttttcgattc gttgatccgc aacatctatc
     5521 tcttgcacgc aaagtaatcg gggaagaatt gatccaaaaa aagaccaatc taagtgatag
     5581 aaagcttgaa catttggttt tgatgctgac attgacgatg gagcgtgtga ctaaaaatca
     5641 tttactgaca aatgagacat ataatagtct ggcaaacaaa gaattactac agacagctaa
     5701 acgattgttt tcacggatcg cttctgagac aaaacaactt tatccagtaa atgagatcgt
     5761 cttttttgca ggattattaa atgacttcac gaactcattt gatcagaatt tctttgaaga
     5821 gacgttcgat acacagcttg cttatttagt aaaacaactg attcaagaag taagtgaaga
     5881 gacgaatgtc catttttatg aagatgaaac tttgtacaaa atgctgttga ctcatttatc
     5941 tggtgtgttt tcaagagccg ttttacaaga agagacactg accaatccga ttttagaaaa
     6001 aatcatggtt caatacgagg aagttgcgaa tgcaatccgt aaagcagtag agcggatttt
     6061 tcaagaaaaa aaattatccg aagaagagat cgcttatatg gtccttcatt ttgcgaattc
     6121 attggaacga agtccgaaag aaagcaatat cagtattgca ggattttcgc caagtggatt
     6181 agcttctact agcatgttgg aactacgatt gaaaaggtat tttcccttca tccaacaaat
     6241 tcatttctat cggattgctg atttaaaaaa aatcgattta caagaagaat atgatgttgt
     6301 tatttctaca tctattttac caggatacgg tggaaaatat attttagtct ccccgctact
     6361 gctagaagat gaagtcaagc agctaaaaga agctttttcg catattgaga agaaaaaaag
     6421 gcaaaaaatt acgtataaca gcaataacga gtggatgata gaagatactt atgaggaaac
     6481 aatgcatttc atggatcaaa tcaatcaatt actcggtcgt ttttttatcc aagaactgga
     6541 taatcctgag acgatttccg aaacgattcg cagcttattt ccttattttc ctgaaggact
     6601 agtaacggat gaagaggtgg ttcatcgccg gctgcttaag cgatatgaac aagcacctat
     6661 cggtattcct catacaaata tggggttatt ccatacatcc agctcattga tttctaaacc
     6721 gatcttttgt attttcaatt tgaaacatcc gttgaccatt gaagggatgg atagacagcc
     6781 agtcgatttg tcccgtatgt tagttatgct ggcgccttcg ccaatccaag aaaaagacgg
     6841 gattctttta ggaaagatca gtggagcaat catcatgaat gacttgaaca ccgagatctt
     6901 ccgctcagga aataaagaaa tcgtttatca gttgttagcg aaaatcctga ttgaagaaat
     6961 aaagaaatga aggtgaaaag atgatacagc taccagcaga aaaaatattg ctcgatcaat
     7021 cctatcaaaa ttggaaagaa gcgttgaccg agatcggcca gtggatgacc caacaagggg
     7081 gaatcaaacc ggcatatatc caatcaatga tcgaaagaca agaaaaagcg agtgtctata
     7141 tcgggaattt cgtagccttg ccacatggga ctgacaaaga tgaagaagta gtagaagaag
     7201 gaatctattt agtccaagtg cctgatggtg tggactttgg aacaaaagag gaaccaaaaa
     7261 tcgcgacact cttatttgta gtggctttaa aaaaagaaca tcagttagcg gtgctccaag
     7321 atttagcctt tttctgttca gacatcgacc aagtaatggc gctcagtgat gcgaaaacca
     7381 ttgaagaagt ccaaatgatt tttgaacatg gacatatata accaatgttc acagtaaaaa
     7441 aagctgcctt gcatcaagta ataacttcaa ccaagataaa aataagagga gctatttttg
     7501 ttgggaggtt cttattactg cggatgcaag cttttgaaaa ctatttattc tagaaaaccg
     7561 tttatttgaa ataggggacg gtgacaaggt ttttgtcacc gcccctatta tttttgtgtt
     7621 ttaatagctc ttttttatat agatagtgcc catacctatg aataataaaa cgatactcca
     7681 tataccaagt gtcaagatac cattcatgaa agtaacctgt tctgaagcta gtggcgagaa
     7741 aatatctcct cctatgatca cattttgaaa atcaacataa gaagttggca agtaatgact
     7801 gattgattct atccatccaa tcgaagaaaa caagcgatac tctgctaaga aaagacaaag
     7861 aagtgtacct aaaagattga ctagagaatg ttcaaataaa gcagatagaa gaaaactgat
     7921 tgtacttaaa aagagcaccc acaacaacag gaacagaaag ctctggaaaa gatactcata
     7981 catcggaagg attcgtatct cctgaccaat gatcgttcct acaggataac ggaagtctcc
     8041 caaaccattt ttgattatta ctagtataga gacgatcaaa aatggcatca cgagacttag
     8101 catagctgca ccataataaa tggtaaaacg gatgctatgt ttctttacca tactatccgg
     8161 taatagattg actagagaga ttacctgctt tcttttttca taagagatga tggaagaaag
     8221 aaacaaggcg gttatacata aaatcaaaaa actagaaatc attcctgaaa aaatattttc
     8281 gtagtaattt gctaaaggga gatgcgctgg caagtcaaaa atagaatatc tttggatctg
     8341 atgttctttc atataacgga gcaactcgac acgtttttgt tgttcaatga ttggaattcc
     8401 ttgtctttga ccagaaatca gtcgatccag tatatctttt tcataatgat aagttgcttc
     8461 gaccatttgc tggacattcc ctgtgttttt tgcatgtaag attgcttcga tcagtttgtt
     8521 gcttttctct aaactttcca caaattcttt ctcattctca tcttctttca acgtttcaat
     8581 caaacgttgc atctcttcat agttatgatt cagttcatcg aattctgtat aagcaagttt
     8641 atgatcaaaa aaaagtggga atgcaaagag cacgaccaac aatagaggaa ataaaaagtt
     8701 tactttgttt tttagtaaga gattgcgttc aaatgtaagt aaagacatag ttacacctca
     8761 tataatttac gataaatagc taaaatctct tccatatttg aggattctgt gacgatttct
     8821 ccatttttta gaaaacatgc tttagaacaa atcgattgga tattcgcgat agcgtgggaa
     8881 gaaagaagaa tcgtcttccc ctctttctgc cattgtctga tgaggctatt caagatatca
     8941 atacttcctg ggtctaatcc gttcaacggt tcatcgaata acatgacaga tgaatcactg
     9001 atcaagtaag cagcaagcaa gacatgttgt ttcattccta gagacatttt tccaatcggc
     9061 aaagtaaggt actcagtcat cttcaatcgt tggaaaacag tttggcattc tactttgctc
     9121 tgccaatagt atttgatcaa agtcaaataa tcaacagccg atagatagtc ggataactga
     9181 ttcgttgatt ctagaaaaaa gaaagaccgg cgataatctg tataagcagt aggagagatg
     9241 ttgtgaaggg acaattgatc ataagttgtt ggcagcagtt gggcaatgcc attcagcaaa
     9301 gttgttttgc caaatccgtt cggggcaatc aaagcagcta cttctcctga ttctactaaa
     9361 agattcaatt gtcgaatcac acgtttttta ccacgtttca tgttcatgtt tttgatcagt
     9421 aatgtcatag ataggcctcc aatataaaat ttaataacat aatagaatgg aaaacatcta
     9481 aattaaatgc taacaaaagt tattgtttgc atattataaa aaaattaaaa taacagtttt
     9541 atttaaaatt gaagttttct gataccgaaa tctttttatt ttgctgacat actcaaacct
     9601 ttggcacaaa gtttcgtgcc aaagtacgac tatttctctt tttagtaagc ggatacaatt
     9661 agagtatcaa aaggagagag ggagagacaa aatggaacgg acgattccca aaaacaacat
     9721 ttcgctaaaa gcgagggtac aaaaattagg cagtacttta tcaagcatgg tcatgccaaa
     9781 tattggagca ctgattgcct ggggtgtatt aacggctcta tttatcccag atggttattt
     9841 accaaatgag gctttggcta ccatggtaag cccaatgcta acttatctga ttcctttatt
     9901 gatcggttat actggcggaa aagtaattgc tggtgaccgc ggatcagttg ttggggcaat
     9961 tgcaacgatg ggtgtgattg tcggaacaga tattccaatg atgctaggcg cgatgatcat
    10021 gggacctctt ggtggatatg ccatcaagaa atttgatcag ctattccaaa aacggatcaa
    10081 atccggtttt gaaatgctag tcaataattt ttcagcaggt cttatcggtt ttggattagc
    10141 gttattaggt ttttctgcga ttggtccagt tgtcgatgca ttgactcaag caatggcaaa
    10201 aggtgtagag atcattttat ctgcccattt gattccattg acaagtattt ttattgaacc
    10261 agcaaaaatc ttatttttga ataatgcaat caatcatggg attttgacac cattgggaac
    10321 tgaacaagta gcagaggctg gaaaatctat tctattcttg cttgaagcaa atcctggtcc
    10381 aggacttggc gtattacttg cttttacttt gtttggcaaa ggagcagcaa aatcttcagc
    10441 accaggagcc atgatcattc actttttagg cgggatccat gaaatctatt ttccttacgt
    10501 tatgatgaaa ccgttattat tcttatcagt tataggcgga ggaatgtcag gaagtttcat
    10561 tttccaattg atggatgctg gactaagagc gccagcttct ccagggtcga tcattgcgat
    10621 tctagcgatg acaccgttca attcttacct tcctgttatt ttgggcgtga ttgttgccgc
    10681 agctgtttcg tttggtattt ctgcagtaat tttgaaagcg gatgcgaaag atacaaatga
    10741 agacttcgaa aaatctgttc aagagacaaa ggcagctaag caagcttcca aaggtgccca
    10801 aggtattcct caaggagcta cattctctgg cgtacaaaaa atcatttttg cttgtgatgc
    10861 aggaatggga tcaagtgcga tgggcgcttc gatcttgaga aaaaaagtac aggaagcaga
    10921 cttgcctcaa acggtgacca atcaagcaat cagccaactg acagatgact cgaatacatt
    10981 gatcgttact caggtcgaac tacaagaaag agcaaaacaa aaagcaccga gcgcgcgttt
    11041 tgtctcagta gaaaactttt tgaattcacc tcgatatgat gaaatcattc aagaattaag
    11101 cagcaagcag aaaacatcag tttctgaaat tgagaatgag ccagcagaag agtttctttc
    11161 agcaaacatc aaacaaatca aagagattct acttgtccac gatgatcgtt ccggatcagc
    11221 aacaatgggg atgaccgtgc tcaaagatat attagacaag aaccatttaa ctatcccgtt
    11281 aagaaaagtc aatatccaag atttaaaaga tgaacctgat tcactgatcg tagccaaaga
    11341 aagtttagcg gaaaaagtac agatggctgc acctcaatcc attcatttat cagtcaacag
    11401 catggtcaat ccgcaaaaat acgaatcgat tgcacaatat ttgaaacaga cagcttaagg
    11461 gaggaaaaca gcatgcaaag tttgccattc gacatgatcg agctcaatcg atcttttaaa
    11521 accaaagaag aagcgatccg ctattgcggt cagatgcttg tagaagcagg gtgcgttgat
    11581 gaggaatata tcgatgccat ggtagataga gacaaaatgt tatctgttta tatggggaat
    11641 tttatcgcga ttcctcatgg aacagatgaa gcaaaaaaat atgtgaaaaa atccggcatc
    11701 tgtgtcgttc aagtaccaga tggtgtagat tttggtaccg aagaagaaga aaagattgcg
    11761 actgttttgt tcggtattgc aggagtcgga gatgagcatt tgcaacttgt acagcaaatc
    11821 gcactttatt gcagtgatat ggacaatgtt gtccagctag cagacgcgct cagcaaagaa
    11881 gaagtaacgg aaaatctagc aattgcttaa ggagagagaa aaatgagagc agtacatttt
    11941 ggtgcaggaa atatcggtcg aggatttatt ggagaggttt tggcagctaa tggatttgag
    12001 attactttcg tagatgtgaa tcacacgatc attgatgctt taaatgaaag aaaagaatac
    12061 gaaatcgaat tagcagatga atcaaaagaa aaaatcactg taaaaaatgt caaaggaatc
    12121 aacaatcaaa caaatccaga aaaagtaatc caagaaatcg ctgaagcaga tttagttact
    12181 accgccattg gcccgaatat cttacctttt attgctgagt tgattgcaaa aggtatccaa
    12241 gaaagagaaa aggaaggaag cacaacatct ttagatatca ttgcctgtga aaatatgatt
    12301 ggcggttcag catttctaga aaaagaagtg tataaatatc ttccggaaac atcatttact
    12361 gacaaatata tcggctttcc aaatgcagca gtcgatcgaa tcgtcccatt acaacaccat
    12421 gaagatcctt tattcgtcca agtggaacct ttcaaagaat gggtcattga cgatagccaa
    12481 agaaaaaata aaaaaatcca attaaaaggg gttttatatg tagatgatct agaaccatat
    12541 atcgaacgca aactatttag tgtcaatact ggtcatgcga ctgtcgctta tacaggtgcc
    12601 ttgttaggtt accagaccat tgatgaagca atgcaagatg cgttagttgt ggcacaatta
    12661 aaatcagttt tgcaggaaac aggaagtttg ctgattgcca aatgggggtt tgatgcagag
    12721 cagcatcatg cgtatattga aaaaatcatt catcgcttcc aaaacaaaaa tatctctgat
    12781 gcgatcacac gtgtcgctcg gacaccatta cgtaagttag gttatcaaga aagatttaca
    12841 cgtccagtga gagaactaca agaacataac ttgacttgcc cacatttgac ggcaacaatg
    12901 gggattattt ttaattacta tgatcctgaa gatgaacaaa gccgtcaatt gcatgaaatg
    12961 aagatccatg aaaacttgga acaattgatt caagaagtca caggaatcaa tgatccgaaa
    13021 acaatcggga atatcaagca aaacgtgaac cgttatgcca agcaagtcgc ttaaaatagg
    13081 atacgaaaaa ggggaatgtg acgaaatttt t
//
LOCUS       DARDHU010000075        12943 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000075 DARDHU010000000
VERSION     DARDHU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.075"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SDD03_002071"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SDD03_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG4304507.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="SDD03_002072"
     CDS             complement(1195..3036)
                     /locus_tag="SDD03_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG4304508.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="SDD03_002073"
     CDS             complement(3218..5533)
                     /locus_tag="SDD03_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4304509.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="SDD03_002074"
     CDS             complement(5718..6578)
                     /locus_tag="SDD03_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG4304510.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="SDD03_002075"
     CDS             complement(6637..7158)
                     /locus_tag="SDD03_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG4304511.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="SDD03_002076"
     CDS             7436..8197
                     /locus_tag="SDD03_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4304512.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="SDD03_002077"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="SDD03_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG4304513.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SDD03_002078"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SDD03_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG4304514.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="SDD03_002079"
     CDS             12557..12913
                     /locus_tag="SDD03_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4304515.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DARDHU010000076        12736 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000076 DARDHU010000000
VERSION     DARDHU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.077"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="SDD03_002080"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="SDD03_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG4304516.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="SDD03_002081"
     CDS             complement(324..893)
                     /locus_tag="SDD03_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG4304517.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="SDD03_002082"
     CDS             1079..1981
                     /locus_tag="SDD03_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4304518.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="SDD03_002083"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="SDD03_002083"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG4304519.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="SDD03_002084"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="SDD03_002084"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG4304520.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="SDD03_002085"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="SDD03_002085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG4304521.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="SDD03_002086"
     CDS             8549..8734
                     /locus_tag="SDD03_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG4304522.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="SDD03_002087"
     CDS             complement(8796..9992)
                     /locus_tag="SDD03_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4304523.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="SDD03_002088"
     CDS             complement(10162..11598)
                     /locus_tag="SDD03_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG4304524.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="SDD03_002089"
     CDS             11891..12700
                     /locus_tag="SDD03_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4304525.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2674 c         2249 g         4009 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctagtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc acgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgaca
//
LOCUS       DARDHU010000077        12322 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000077 DARDHU010000000
VERSION     DARDHU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.078"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            30..1391
                     /locus_tag="SDD03_002090"
     CDS             30..1391
                     /locus_tag="SDD03_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG4304526.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1413..1673
                     /locus_tag="SDD03_002091"
     CDS             1413..1673
                     /locus_tag="SDD03_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304527.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            1687..2439
                     /locus_tag="SDD03_002092"
     CDS             1687..2439
                     /locus_tag="SDD03_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG4304528.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2533..3138)
                     /locus_tag="SDD03_002093"
     CDS             complement(2533..3138)
                     /locus_tag="SDD03_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4304529.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3191..4165)
                     /locus_tag="SDD03_002094"
     CDS             complement(3191..4165)
                     /locus_tag="SDD03_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG4304530.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4543..5262)
                     /locus_tag="SDD03_002095"
     CDS             complement(4543..5262)
                     /locus_tag="SDD03_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4304531.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5389..6660)
                     /gene="celB"
                     /locus_tag="SDD03_002096"
     CDS             complement(5389..6660)
                     /gene="celB"
                     /locus_tag="SDD03_002096"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG4304532.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            6813..8210
                     /locus_tag="SDD03_002097"
     CDS             6813..8210
                     /locus_tag="SDD03_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4304533.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8291..8731
                     /locus_tag="SDD03_002098"
     CDS             8291..8731
                     /locus_tag="SDD03_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4304534.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            8879..9205
                     /locus_tag="SDD03_002099"
     CDS             8879..9205
                     /locus_tag="SDD03_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4304535.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9206..9955
                     /locus_tag="SDD03_002100"
     CDS             9206..9955
                     /locus_tag="SDD03_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304536.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10035..10490)
                     /locus_tag="SDD03_002101"
     CDS             complement(10035..10490)
                     /locus_tag="SDD03_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG4304537.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(10917..11192)
                     /locus_tag="SDD03_002102"
     CDS             complement(10917..11192)
                     /locus_tag="SDD03_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304538.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11486..12301
                     /gene="thiD"
                     /locus_tag="SDD03_002103"
     CDS             11486..12301
                     /gene="thiD"
                     /locus_tag="SDD03_002103"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG4304539.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         3934 a         2129 c         2506 g         3753 t
ORIGIN      
        1 ctatatttag aaagaaagaa ggtttattta tggaagtttt ccaaaccatt atcaattatg
       61 ttttgaactt aggatcagcc atttttgttc ccctgattat tttgatactt ggattgttgg
      121 cagggatgaa attaaaaaaa gcattcatgt ctgctttgac tttagggatt gcttttagcg
      181 gaatgtctat ggtcattggc tatatgtcta acgcagtaag tccagcatca gaagcattgg
      241 ctaaaaatac gggaatcagt ttgcctgcac ttgatcttgg gtggacaggt gcagcaagca
      301 tcacatggtc gtggagttat gcttttatat tttttgcagt aacgattggg gtcaattttg
      361 tactgttact ttttaattgg acgaagacgt taaatgttga tatgtggaat gtttggggga
      421 aagctttaac tgcttattta gtttattatg tttccggtag tttggctgca ggctttctta
      481 ctgcaatggt ccaagttata ttggaattaa agcttggtga tatgtttcaa aaacatatac
      541 aagatctcac agggattcca ttagtaacag tgactcattt tatgacatct gccgctgtct
      601 tgttgctgcc tttcaatatg atcatggata aaattcctgc tttaaataaa cgagcggata
      661 cgaatgcatt gaagaagaaa attgggatct tctctgaaaa ctctgtaatg ggattcatta
      721 ttggattggg gcttggcttt gctgctgcgt atggcgtttc aggttctctt aatctagcga
      781 tccaaacagc tacagcatta agtttgtttc ctatgatctc taaaatgttc atgcaatctt
      841 tgtctcctct agcagacgct atgtcagaat ttatgaaaaa gcggtttaag gatcgtgaag
      901 tatatatcgg attggactgg ccgatattag ctggtcgttc agaaatttgg gttactgcta
      961 ttttacttgt tcctgtgttt attggttatg ccattatttt gccaggaaac caagtattgc
     1021 cattagcagg gattatcaac tattcgattg cggtcggcgg attactttta acaggaggaa
     1081 acttggctcg tatgctgatc ttgggaatca tcactatgcc tatttatttg tacggggcca
     1141 cttaccttgc gccaatactc agtgatttag cagaaaaaag cggagcagtc gagggaatca
     1201 agaaaggaca gttgatcaca tggtcatcca tagaagggcc ggagttcagg gttttatttg
     1261 cggaggcatt taataggaat attctcgcta ttgtaggatg cgtgattttt ctaggactgt
     1321 ttgttctact ttataaatat atgataaaag cgaaagtacc gagtgaacgt tatgctgaac
     1381 tacaaaaata gcattgaaat ggtgggaaag taatgaaaag agaagagaca gataaaataa
     1441 aatggacagt cgctctttgt ggtacacttc tgctatttct atatggactc tttactcaga
     1501 atatcatcat caatctattg gtcatttttt tcgcactagt gatctataaa tatggaaatc
     1561 acgttttatt cagagagtac gatgaaaaaa gaaaacggaa aatagaagaa tcaatgaaaa
     1621 taaaagaagc taccaaagaa atattgagag aaaaaagttt tataaaaaga taatggaggg
     1681 gtcattatgt cagatggacg tatgttattt gagagagaac gacaggacat ggcacgtatc
     1741 gtaagagtga ttttcgaaag gaaaaacaca aatgtcgcag gagggaattt ttcctttaaa
     1801 accactgatg atcaagggaa agactacatc atcatgacgc cgaccatgat gtcagaagcc
     1861 tatctaggag agatttctcc tgcacaggtt ctagtagtag aaccaagaac gcgtagaatc
     1921 gttgacggag tcggtcattt aactcgtgaa atcaatatgc atgaagcgat gtatgagact
     1981 aatccagcta tcaaagctgt cctacatgct catgcgccta atgcaatgtt ctgggcgact
     2041 actgggttgc ctatgcctaa tttgacagaa gccacagcta aagtaaaaga gattcctgtc
     2101 atggactttg agcctaattg ctctgaagaa cttgctgaat tgacaagcga ctatgtaaaa
     2161 aaacagcatc tcgaattacc gcatatgttg ctgctcaact ctcatggcgt attgatcaac
     2221 acaggtggag aatcaggaat cgaagctatc cataaagcac tgcaaatcct tgatacagtt
     2281 gaatggaatg cagaaattgc ctacaagcag acgatttttc aagcattagg gattactgac
     2341 ggtttttatt caaaaggtga gaaaatcggg ggattggaag agttgaaaca taaaacggct
     2401 atttataata aatctgttat tgcagctggt ggagactaac ctatctgaca caaaaaacta
     2461 cgactttcac aaaatctgat ccagatcgat atctgcttca ggttgtatta tgtaagtcgt
     2521 agttcttttc ttttatttag ttaagaggtg gtctgtccat tccctcgctt gcttgatttc
     2581 gtccatcgtc aactgatgac ctgttacagt tagctgaata gtcacatttg cttggcgtgt
     2641 ttcgaattga tcggccaatt ctttaaagga agcaggggaa acgattggat cgttatcacc
     2701 ataagaaatc cagacggttt tatcctctaa tgtggaacgg ctttgatgaa cacctagaga
     2761 catcggatgg aaaaacaacc ctttatttag ctcggtctca cgctccaata ataaatgagc
     2821 agcgatgttt gcgccatttg aatagccgac aagcagccag tcacttaacg gtatcagttc
     2881 tttttcgctg atttctttga ttttttcatg gagtttatct gtttcttttt ccaaactgtc
     2941 caggtcgaat tgattcaatc cattacgctt gaaaaaacga ttcatcccat cttcttggac
     3001 cgtgccacga aaagaaagca atgtactgtt tggtgcaaga aagtgagcga tatccagtaa
     3061 agaatgttca tcgccacctg tgccatgaag taagatcaaa tgcttgccac ttgttccttt
     3121 ttcaaaaatg taggtcattt tgttccctct tttctgtttc tatagatgca aatgaagtag
     3181 catgaagaga ttattcacgt tttttatttg catcacttgt atcgaaagga cggacataag
     3241 cttcaatggc ttcacgacgt tcttctaaga atggaggaag agatagtttt tctcctgctg
     3301 attctgctgg ttcatcttca aggaatccag gaccatcagt tgctaattca aacagtacgt
     3361 gatgagcggc taggaagtac tcagattgga aatagaaacg atcgacgaaa ccagaatttg
     3421 gcaattcaat cttattgatt acttgaatcc agtattccaa tgcttctttg tctgctacac
     3481 gcaatgctaa gtgatggata tttccaaatc cttcgattgc ttttggcaag tctgtccgat
     3541 gttcaataat gatgcttgca ccatttcccc cttcaccgat ttcaaataag tagaaatctc
     3601 ctgaatggtc tgtttctttg aatcctaaga ttttttcaag aatcaagcgg atatgctcta
     3661 attgactgac acggacaaag acaggtccaa gtcctgtgat cgcaaactct gtcggtacat
     3721 tgctgttttt ccaaggaatg ccagctgcta caccttgatt gttttcgtca gagatcaatt
     3781 gataatactg attatcgaaa tcttcaaatt cgatatattt tttgccgaaa cgttcattga
     3841 ttgctgaatg agtaacgtca tatttttcaa aacgttccaa ccaaaagtct aatgctgcgt
     3901 cacttggcac acggaaagaa gcacgagaga tcgtatctgt tccttttttt ccttttggct
     3961 gatttggaaa atcaaagaaa gtcatatctg ttcctggcgc accagtatcg tctgtaaagt
     4021 aaagatggta agtttcgatg tcatcttggt taactgtttt tttgattaag cgcattccta
     4081 aaatatctgt aaaaaagcgg tagatttttg tagcgctaga tgtcattgct gtgacgtggt
     4141 gaattcctct gatttgtgta tccatgataa atttcctcct gttaacttac aaaaagtaag
     4201 tagttgattt agacatagta tagagggtgc atctctattt tgcaattaat ttgtcttcta
     4261 aagtatcagg aaggaatcaa gaatggaaaa gaggagggac ggatcaataa aataaaaaga
     4321 cttacgacta gctgtatcga tttcaaaaaa actagcttta aaaagctaac tttagaatca
     4381 ttacgggtgg tcataagtct ttttatgctg agttattttt tcatttttga gctaatgctt
     4441 aggatagaat agaatgttga tttcacgatc tcttcttgtt acttgaaact gcttaccact
     4501 gtcacgatct cttggtaaac ggctttttca caacctcttt ggttaagttg aacgcacatc
     4561 cagcacagaa tagttccgaa catcaaatcg gtttcggctg gaagaatatt caatcggctg
     4621 tccatctttg agatagacga cttgctgaac ttccagtaca ggatcgtttt ctgcacagtt
     4681 caaatattgc tgatcatatt catcactttt atctgcagaa atagcacgat aagcccctgc
     4741 aaaatggaca ttcaattctt gatgaagatg atcatagata gaggctaaca aatgttcttt
     4801 ttttaaaccc ggcacaagat ggactggcat aaaggtatgc tctaaaacat atggctcgcc
     4861 atctaagatc cttaaccggt gaatattgta gacaggttgc tcggcatcga tcatcaatcg
     4921 ttcttggata tattgattag ggaattcaac atcaaaatga atgatttgac tcttcaaaga
     4981 acgatgtctg cgtttcattt ggagactcaa accttcatac tcgttagcgg gcgatgtgtc
     5041 tttttgcata aatggatgat tcaagatttt cgttcctgat cctcgctgcg aatagacaag
     5101 tccttccatt gctaagatat tgatggcttt tttgattgtc atccggctcg caccaaattc
     5161 ttttaccaag tcgacttgat taggcaaaaa actatcgaga ggatactctt ttgaacggat
     5221 acgttctctt aaaatatttg cgatttcttc gtattttggc acgttattca cctcttctag
     5281 agtataacga aagattatgc ttccgacaac tcgaagtagc atctggaaca ttgtttattc
     5341 gaaaaaagag gtcatgacaa gtttttgtca caacccctta atgatctatt aagcaatatc
     5401 taaatctggt tgtttatcta gtactttcag gaatgggtac cagataaagg caacgatgac
     5461 aaagtcgatg acttgcagca taccaccaag ccatgaattt gttgctaaga caccgcttgc
     5521 aatgattggc acagtccatg gaacagagac gccagttggt gcagggacaa gtccagcagc
     5581 cattaccaaa taattgaacg tcgttacgat tagaggtgcc acaatccaag gaataaggat
     5641 tgttgcattc aatacgatcg gtagaccgaa gatcgctggt tcatttacat tgaaaatacc
     5701 aggtgctaaa gctaatcgtc caacatcacg atactgtttc tttttaagaa caaaggccat
     5761 caataaaacg acagctaaag tcattccaga gccaccaata ccaacagtga aggtttccat
     5821 gaatggtttt gtgatgatgt gtggtaattc ttttccatgt tgataagctt ctaagttatc
     5881 aagcatcagt gtattccaaa tcgggtccat tacagagttg acgatgattt gtccgtgaag
     5941 tccgaagaac cataagaatt ggacaaagaa caaagcaatc aatgttgcag gtaaactact
     6001 tcccaaacca gttaatggtt tttggatcac ttcataaacg acgtcatgta agttagcatt
     6061 gaaaatacca gtcatcacag cattgattac taagaaaata gttagtgtca ctacagcagg
     6121 gatcaaagcg gcaaatgatt ttgttactgc aggcggaact ccatcaggca ttttgatctg
     6181 ccagccgcgt ttcgtgattc tgcagtagat ttctccagcc aagaaagcag cgatcattcc
     6241 aatgaacatt ccttttgcgc caagacgatc aagtgaaagc acaccagtca catcttcacc
     6301 gccagcggtt ttcattgcaa aaggcgtaag aatcaagaag ctggcaaaag acacggcacc
     6361 accgaaaata ccttcaacgt cataagattt tgataaataa tagccgatac caaaagtaac
     6421 aaaaaccgac atgatgctca ttgttgcatt ttgcccatta ccgaataaat tcgataaagt
     6481 tccctttgtt gcgtcactga aaaaaggcaa gttattgata acgacaacga tcgaaccgaa
     6541 catcgttaaa gggaaggaaa gcatgaaagc atcccgcagc actgtaaggt aacgattttg
     6601 ccctaataaa ttagctaagg gcatgatttt ctcagaaagc ttgtccataa aaccgttcat
     6661 ttttattcac ctcaaattta tttgcaatcg attacataca aagtataacg ccaaaaattt
     6721 taaatgtcta gtcattttca taaaaagtat tgatttattt tttagggttc gttatgatta
     6781 cggtatcaag aaaaaagaga ggaagtaaaa aaatgaaaac ctacacattc ccagaaaatt
     6841 tttggtgggg ttctgctgct agcggaccgc aaacagaagg aagagtaagt gatgatggta
     6901 aaggagaaaa tatttgggat cattggtatg aattagagcc tgaaaaattc ttcgatcaag
     6961 taggacctga aaaaacttcg caagtttaca caaaatacaa agaagatgtt cagttaatga
     7021 aagaaactgg ccataacaca tttcgtacat ctatccaatg gagtcatttg attccagaag
     7081 ggacaggtga aatcaatcaa aaagctgttt ctttttatcg agattatttt actgaattgc
     7141 tggataatgg tattgaacct tttgtgaacc tttaccattt cgatatgcca ctaccactgc
     7201 aagaaaaagg cggatggtta tcaagagaaa cggtcgacgc ttatgtttct tatgcagcag
     7261 cgtgctttga actgtttgga gatctggtca aaaaatggtt tactcataat gaaccaatcg
     7321 tacctgtaga aggaggctac ttgtatcaat tccactatcc aaatcatatc aatatgaaag
     7381 aagcggtcca agtaggctat catgagaatc tagcaagcgc aaaggtaatc aaagcctacc
     7441 atgaaggcgg acatgatggt gagatcggaa tcatcttgaa tctgacacca agctatccga
     7501 gagatgaaac gaacccagaa gatgtaaaag cggcgaagat cgctgacgca ttcttcaacc
     7561 gatcattcct tgaccctgca gtgaaaggag agtttccaca agagttgatc gaaattgtca
     7621 aagaattggc tataatgcca gagatccatg aaggagacct agaactcatc cgtgaaaaca
     7681 cgattgacct gctaggaatc aattactatc agcctcgtcg aatcaaagca aaagaaactc
     7741 caatcaatca cgaacatagc cccatgccag atgattattt tgataattac gacatgccca
     7801 ataaaaaaat gaatccatat cgtggatggg aaatttacga aaaaggaatc tatgatattt
     7861 taacgaatac aagagaaaac tatggtaata tcaaatgcta tatttctgaa aatgggatgg
     7921 gggtcgaagg agaagaacga tttgttaaca gcgaaggaat tattgaagat gattaccgga
     7981 tcgagtttgt tacggatcat ttgaaatacg tccatcaagc aatccaagaa ggaaccaatt
     8041 gtgtgggtta ccacatgtgg acatgtatgg acaactggtc ttggacaaat gcttataaga
     8101 atcgctatgg ctttatcgct gttgatttgg cacaagacgg taaacggacg atcaaaaaat
     8161 caggtcgctg gtttaaaacg gtctctgaaa aaaacggatt tgatgcctga tattttttga
     8221 taaaacgttc acaaaatgtt tgttctaaca ctgtaatcgt gctacactaa cagtgaagga
     8281 ggcgtttaca atggtagata cttatcgtaa tattttagta gcaattgatg gttctgaaaa
     8341 ggcagaaaaa gcatttcttg aggcaatcac gattgccaaa cgaaatcagg caacgttgca
     8401 catcctgtat gtcaatgaag taacaggcaa ttactttggt gattttgcat ttgttacaac
     8461 aagccttcag gaagaattgg atgaggttgc ggaaaaccag atgaaagaac atcgaaactt
     8521 ggcaattgaa aaaggcctga cagatatcga gacatatgtt ctttacggtt atccaaagac
     8581 actgattgct aatttccatg aatcgaaaga accaatcgat ctgattgtca tgggggcgac
     8641 aggtctgaat gcggtagaac gtgcactagt aggttccact acttcctatg tagtcaacca
     8701 cgcatcatgt aacgtcatgg ttgtaaaata aataaattaa gggaacagaa agcaaaaaag
     8761 cataacgctt cttttgcttt ctgttccttt tatttttttg taaaaaatta ctttacctga
     8821 tgaagtaatc atgctattct aattacaacg acagatggag taatatggag gaaagaaaat
     8881 gatttcaagt gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa
     8941 aaacgattct tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta
     9001 tgtgatcaaa gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat
     9061 tgtttcgtat cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga
     9121 aatagggcgt aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt
     9181 cgatcaattt ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt
     9241 tatcggagta cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat
     9301 ggaagaccat tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac
     9361 agtaatttcg acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa
     9421 acatcaagct gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa
     9481 ctactggaaa gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag
     9541 cttatctttt gcaagttttc ttttcttttg ttcagcagga tatgtgttca tgggtatttc
     9601 ttgtctgatt ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat
     9661 cacacgttta aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc
     9721 aaaagaaaaa gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg
     9781 attatgtatt ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag
     9841 tatcggtgcc tctatatttt ttgttacggt aggaacgggt gtcttcttga tcatttatgg
     9901 cagtctagtc cgtcattctt atcgtcaatt cactcaaagc gcttattact ggtgaatgta
     9961 ttcagatttg taaaaatatt ctatttatcc ggcgtagaaa aaaagactgt gacacgcttt
    10021 tgtcacagtc ttttttatat ataataattt aaaaattcat catctaaatc cgtagaattt
    10081 tctagattgc ttacccatgt aaaatcgtta tagacagatt tcagttgact tctgcgccat
    10141 tgtacaggga taaaaagacg catgctatct tcattgaatc cgactaataa tttctttccg
    10201 tcagtaatga tgggacgttg taacaaagta aaatgcgaga cgatcagatc gacaagctct
    10261 gaggtcgtac attggtcgat ttggtctttc aaatttttat aatcaatcga gcgtttagct
    10321 aatagatcgt caaatccgtt ttctgttaat gacaataaat aaaatatctc tttccttgtt
    10381 aaaggctgtt taggtgtgaa aattcgttct ctgtaaggca tttgatggtc tttcagccag
    10441 tttgccatca gatggtaact atgtgtgcga gaagaataga tcgtaagcat aaaaaaactc
    10501 cttaaatagt ccattaattt gttagatctg ttgagataaa taataggtaa ggagctgtgc
    10561 ataagcattg atcaaaacaa cccaaacatc attagaaata actttctaaa gtcttaaact
    10621 tgcgacgaga aacaactaga ctaatagaaa aaatgtctta gccatttggc ttcctgtttg
    10681 tttttttgct tatgctgcag ctcctcggga aagggttcta acaatttgaa gaaggttgat
    10741 gattgatcta gtttaaattg tgtgaaattt gaaatctggt gtttttacta gatgaatcac
    10801 ctaacaacta tgattataac ttatagaact tctgttttgc attcggtttt gttatagttg
    10861 ttataaagtt ttattttatt acagaacatt tttaattaat aaagaacagt tagaagttaa
    10921 aattgctgat tcacatagag tttatgactt cttggaaatt ctttttcaat cagttttttc
    10981 atctgtttat gaaattcttt tttttcagta aacataaagg tttgggtatc gtaaccttct
    11041 tcatgtagga aatagattat tcctttttct gtgttttgcg tataagcttt tatactcctg
    11101 tcttttttga aagaacagat cgtatagcca tctatttggt ctggtagtcg tttgattttc
    11161 ttcaaaatga cttttttatc ctcaagtttc atttttttca cctgacttta tttttttcat
    11221 atggaaatca tagcgccttt ggttatatga aaacaagaag gatactgaaa tatatataaa
    11281 tcaaatagtg gttatttatt attagaatag cgaatacaaa taaacgacca ctattttctg
    11341 atttattgca aatgtttttt caaataaatt tgattaccaa gaaaaggtag tggtatattt
    11401 actgagggat actttgaaaa acatatgata gaaacagtaa cagtattatg aaaaatcttt
    11461 ttataaaaat ggaggaatcg atttgatgga agatcagtat gtacaagcaa tgacgatcgc
    11521 aggatcagat agcgatggaa gcgcagggat gcaagcagat atgcatacat tctttacaag
    11581 aaatgtttac ggtgtttctg tcatgaccgc atgtgttgca ggtaattctt acggaatcgg
    11641 agcaagtgtg acattgccaa cagattttat cgacaaagaa tttgaactga ttgcgaagga
    11701 ctttcaaata cgtgcagcta aaacaggtat gctagcagat tcaaagttga ttgagacagt
    11761 tgtaaaaaat taccaaaaat atgattttgg accattagtc gtggatcccg taatcgtaac
    11821 gaaacatgga aatcttttac tagaagaatc tgctttgcaa agcttgaaag aaaagcttgt
    11881 ccctttggcc gaagtactga cacctaattt ttatgaagca gaaaagctga ctggacggac
    11941 attagcagct gaagaagatt ttgtgcaagc agcaaaagaa ctgcaagata tgggtgcaaa
    12001 aaacgttatg atcaaaggac atcatgctaa aaatgcgcag caagaaacag tcaatgacta
    12061 cgtattgttc gaatcaggaa aatcattttg gttaaatgca ccattttatc ctaccgaccg
    12121 gatcaacgga actggcgatt cgctatctgc atgtattgca gctgagttag cgaaaggaaa
    12181 aacagttgaa caagcgatcc gattggcaaa agactatgta aaccgtgcaa ttcgtgaaga
    12241 aataaatgta gggcacaaat atggccccat caatcattgg gcagctggtg aattagagta
    12301 aaagaaagaa gttgtgaaaa ag
//
LOCUS       DARDHU010000078        12172 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000078 DARDHU010000000
VERSION     DARDHU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.079"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(394..975)
                     /locus_tag="SDD03_002104"
     CDS             complement(394..975)
                     /locus_tag="SDD03_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4304540.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1158..1784)
                     /locus_tag="SDD03_002105"
     CDS             complement(1158..1784)
                     /locus_tag="SDD03_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4304541.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1806..3137)
                     /locus_tag="SDD03_002106"
     CDS             complement(1806..3137)
                     /locus_tag="SDD03_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG4304542.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3292..3693)
                     /locus_tag="SDD03_002107"
     CDS             complement(3292..3693)
                     /locus_tag="SDD03_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4304543.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3779..3985)
                     /locus_tag="SDD03_002108"
     CDS             complement(3779..3985)
                     /locus_tag="SDD03_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304544.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4080..5582)
                     /locus_tag="SDD03_002109"
     CDS             complement(4080..5582)
                     /locus_tag="SDD03_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG4304545.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5600..6055)
                     /gene="argR"
                     /locus_tag="SDD03_002110"
     CDS             complement(5600..6055)
                     /gene="argR"
                     /locus_tag="SDD03_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG4304546.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6153..6770)
                     /locus_tag="SDD03_002111"
     CDS             complement(6153..6770)
                     /locus_tag="SDD03_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG4304547.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6991..8364
                     /locus_tag="SDD03_002112"
     CDS             6991..8364
                     /locus_tag="SDD03_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4304548.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8439..9551
                     /locus_tag="SDD03_002113"
     CDS             8439..9551
                     /locus_tag="SDD03_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG4304549.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9623..11260)
                     /locus_tag="SDD03_002114"
     CDS             complement(9623..11260)
                     /locus_tag="SDD03_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG4304550.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11428..12036)
                     /locus_tag="SDD03_002115"
     CDS             complement(11428..12036)
                     /locus_tag="SDD03_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4304551.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3227 a         2564 c         2192 g         4189 t
ORIGIN      
        1 cgactttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
       61 cccaaccaaa cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc
      121 agggcagctt tttcacaacc tctccactaa acggtgttca atcagctaac cttcggtatt
      181 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
      241 tcaaagcgct gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa
      301 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
      361 gaagctgacc acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa
      421 tctttctttg atcaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa
      481 caataagata cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc
      541 atcggacagt ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg
      601 attgactaat tctagtagca acagacggta ttcttcatcc actgtactca actcaataat
      661 caaattacct acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca
      721 gtccaacatt tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa
      781 caattctgtc tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt
      841 tgaagaaaag taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg
      901 gattgacgtt tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt
      961 ttcctttgtt ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta
     1021 aattcatcat tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag
     1081 ccctcactat aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga
     1141 caaaagcaat tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga
     1201 tctgcaataa agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca
     1261 attgcatctg tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa
     1321 tcgctacaca agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc
     1381 aattggagcg ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc
     1441 gtattttcat ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc
     1501 actgattcat attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct
     1561 gcaccgccaa attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt
     1621 ttgtatcctt tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat
     1681 ttttctaaga tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact
     1741 tgcgggtcag tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc
     1801 gttatttatt ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga
     1861 tctctcaata acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact
     1921 tcatgagaat cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact
     1981 aaaactttga atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc
     2041 atatacttga ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct
     2101 acattggata aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt
     2161 gttcgtttat tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata
     2221 taagtgaact gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta
     2281 cgaaggtatt catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct
     2341 aatccaagtg atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg
     2401 attgttactt cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct
     2461 acttgatcta cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa
     2521 atcatttgcg cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg
     2581 acaactttag atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc
     2641 ccgatgatca ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc
     2701 aagtagcgac ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg
     2761 atgaaaatcc gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga
     2821 tggttagaaa gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc
     2881 atatgataat ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct
     2941 gtaaagtcga ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg
     3001 ttgatacatg ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct
     3061 tttgaagcta acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt
     3121 tgatattttt ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata
     3181 tgataaccga taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga
     3241 caaaaagcgg tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat
     3301 cgttacttct acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc
     3361 atcttctgca attttttggg cttcttcttc gctgatgcca tcgatatgca cttctaatac
     3421 tacaccgatc tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt
     3481 ggactctgct gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga
     3541 agcataacct gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg
     3601 agtgattttc atatgcatcg agccatttgg tgcctcgact tctccattgc gcccgccatg
     3661 gttgatcatt gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt
     3721 gctacttggc tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt
     3781 attcgaatac cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg
     3841 tgaacaacag cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta
     3901 tatgattgat tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta
     3961 aaaaaataac catattcgtt accatttttt cctccttttg ctggtctccc taccagcact
     4021 cgatttaacc ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt
     4081 tattctgctg ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact
     4141 aacgccaact tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca
     4201 tgtaagctta aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga
     4261 ataccttctt cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc
     4321 atttcttcta atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg
     4381 atttcttgta cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct
     4441 tttcttttta attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc
     4501 agaaaatagt tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct
     4561 ttcccaagac ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa
     4621 cgaacttgga tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa
     4681 ttcgtcacga ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc
     4741 aaattatttc gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct
     4801 gtatcgaaaa tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc
     4861 atcgcttctt tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa
     4921 aattgggaac ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca
     4981 ccagcaccat ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt
     5041 gttccaatcc cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct
     5101 gctttttcta cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa
     5161 gcaatatctt ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa
     5221 tagggaagat ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga
     5281 gtggacataa acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt
     5341 tctttcagac tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga
     5401 ccaatcccaa tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag
     5461 tttgctagtc cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct
     5521 tcaactggta atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc
     5581 atataaaagt cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg
     5641 cttcttcttc tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa
     5701 cttcagggta agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag
     5761 tcaatacgat aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg
     5821 tacgcaaacg ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta
     5881 ttttgttttc attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg
     5941 cttgagtcac atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa
     6001 taggttgttc tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag
     6061 acacctctca atgttgatta tagcaaaaga taagtaatat ttcttcatat tttggtattt
     6121 tttactttta ttttttgttt tcattcaggt tgctaacctt tttctatttt aacacgcaaa
     6181 atcgtcttca ttttttcagg tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt
     6241 tctttagaag ctctttcata gccttctttt gcgatgaatg cttctaattt ctcaaacgtt
     6301 gctggttctt cggcataggg cccgacgtgt aagatttggg cacaaagacc ttcttccatc
     6361 tttttgaata ctagttgttc tttcaagtct tccggaagtt tgctagaacg ctgaatggct
     6421 tcttgtgcta tcgcttctgt aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa
     6481 tgttcttttt tcatcactgg ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc
     6541 agtggataga cagtatacat ctgataatca ggaatcagcc aatttttttt aggcgacatg
     6601 cgtattgcat agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc
     6661 gggtcgccga tccctctgat gctaaagaat ggttttttag gaacctctag tatttgcggg
     6721 ctttttttca tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct
     6781 ccgcgaaatt ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg
     6841 tgttgacact atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa
     6901 cacgtttcgg cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt
     6961 tttttacgaa attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta
     7021 cctgcagtaa ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg
     7081 aatgtaactt tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg
     7141 gtaacaacga tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat
     7201 cgcaatttta gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata
     7261 atgatcgatt tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt
     7321 gcagcgacag gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc
     7381 aatcgacgag gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt
     7441 ggccccactt atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt
     7501 cttgtgcccg ttttgattct ttctttgatt atcggctggt ttgctattcc cgagatggca
     7561 gtacaaaaaa gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt
     7621 ggaagtctaa catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc
     7681 ggcggtattg gctggttcct tttttactat cgaagtaaaa aagtggagca tccactgatc
     7741 cgactgtcta ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat
     7801 cagtttttat tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga
     7861 agtagtcctt ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt
     7921 tctccttttt ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg
     7981 ctgattcttt ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg
     8041 ggtgtattga ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat
     8101 atgatgacca ccggaatgaa tgcactgaat gaagaactac aaggagacgg gaacgcaatg
     8161 ttcaatacct tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc
     8221 aattacgtcc agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa
     8281 gcagcattag gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat
     8341 ctgttctttg ctaaaaaagc gtagattcca tatacggcag gtgttgcatc tgccgttttt
     8401 ttgtgtgaaa atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg
     8461 gtggcgggat cattggtatg actctagcca gttacttaga tacatcaaaa ttcaatgttg
     8521 ctttatttga tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt
     8581 ggttgtcgaa acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt
     8641 tccctaaact tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac
     8701 ttttgcttcg acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag
     8761 aagaagcgcc agaaattgga aacatcgaat ggctttcacc tgaacagaca tctcaagcac
     8821 ttccgttatt gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa
     8881 aagcatattt agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg
     8941 caaaagtcac tttgcataag aagagcgatt cttggctcat caagggaccg tttggagaat
     9001 acacagctga tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat
     9061 taggctatgc tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt
     9121 ataagatgag tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt
     9181 tcatggatgg aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg
     9241 ctcctactag cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc
     9301 cagatcagat aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag
     9361 atttttcccc atttttcggc gtactgccag atgatccaag cttactggct gctagcggtc
     9421 taggctcttc tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa
     9481 atactggtca ttggtctacg gaagattatc aaaaggacat ctccacctat attacgaaaa
     9541 atccatcata gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt
     9601 acttcttgaa aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt
     9661 ttgcttcatc tggcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat
     9721 caagtcgttg tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg
     9781 gtataggtaa atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt
     9841 gcacgattct ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat
     9901 atccagtatt caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt
     9961 gatagtcttc cctaagagga tagacacgga aaggattagc aaatggttca atgactagct
    10021 catctagctg tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg
    10081 caccaaaaga cgctgctaaa actgggtcat tgatcttgat aattggcgga agcgaatcat
    10141 ctttcatgat ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg
    10201 tcgcataacg tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga
    10261 ctttttctcc tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa
    10321 tggccggatc atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag
    10381 tagcaaaacc attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt
    10441 gaccttccgt atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata
    10501 cagccatcgt ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa
    10561 taaagccatt gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc
    10621 caaagtaacg taatcctagc acagccgctg tattatggag agcatcaaac aaaatgagtc
    10681 catttggaaa tgtttcatgt gtccattcgg gatcacaaaa gatataaaga tctccctcat
    10741 caaaggaaac agacttttga taagtctcta agatcttcga attcgctggc tgaaaattca
    10801 gcaaataaga atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag
    10861 aaaattcttc agataggcca acgattactt ttgtatggta aaactggcgc ttgcgacttt
    10921 gatacacagc ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa
    10981 agatccgccg agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc
    11041 taacatcagc tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg
    11101 ttcctggact cccttttgct agttgataag ctgtcttcct gtcaaaaact tcatgaacat
    11161 tattaccgta aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac
    11221 ttgtgatttc gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa
    11281 acaaaaaaac gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt
    11341 gggaagcaaa cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct
    11401 cggtttcatc attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt
    11461 agcagcatcc catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt
    11521 ggcagctgaa tagtcgccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg
    11581 tccgttagta gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga
    11641 gcttgaagtt ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac
    11701 aggtgcaacg tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt
    11761 tgttttgttt tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc
    11821 aacataaatg cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga
    11881 gttgtctcct gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata
    11941 aacttcatca gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa
    12001 agttgtacct aaaacaattt tcttaaatga attcatatta aactcctttt tttctccgtt
    12061 ttttattctt tcgacgctat taactttatc atttagaaat atttcatagg taaaagaaac
    12121 atgacattta atgacaaaat aacgaattat ttaatatttg taacatttaa ac
//
LOCUS       DARDHU010000079        11667 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000079 DARDHU010000000
VERSION     DARDHU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.080"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..216)
                     /locus_tag="SDD03_002116"
     CDS             complement(37..216)
                     /locus_tag="SDD03_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304552.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="SDD03_002117"
     CDS             complement(597..1304)
                     /locus_tag="SDD03_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304553.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="SDD03_002118"
     CDS             complement(1297..1734)
                     /locus_tag="SDD03_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304554.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="SDD03_002119"
     CDS             complement(1749..2000)
                     /locus_tag="SDD03_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304555.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="SDD03_002120"
     CDS             complement(2000..2206)
                     /locus_tag="SDD03_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG4304556.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="SDD03_002121"
     CDS             complement(2643..2903)
                     /locus_tag="SDD03_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304557.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="SDD03_002122"
     CDS             2978..3181
                     /locus_tag="SDD03_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG4304558.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SDD03_002123"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SDD03_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4304559.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="SDD03_002124"
     CDS             complement(3810..4709)
                     /locus_tag="SDD03_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG4304560.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="SDD03_002125"
     CDS             complement(4722..5315)
                     /locus_tag="SDD03_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG4304561.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="SDD03_002126"
     CDS             complement(5391..5540)
                     /locus_tag="SDD03_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304562.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="SDD03_002127"
     CDS             complement(5556..7760)
                     /locus_tag="SDD03_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG4304563.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="SDD03_002128"
     CDS             complement(7771..9231)
                     /locus_tag="SDD03_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG4304564.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="SDD03_002129"
     CDS             complement(9292..11664)
                     /locus_tag="SDD03_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304565.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DARDHU010000080        11626 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000080 DARDHU010000000
VERSION     DARDHU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.081"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..629)
                     /locus_tag="SDD03_002130"
     CDS             complement(105..629)
                     /locus_tag="SDD03_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304566.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SDD03_002131"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SDD03_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG4304567.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SDD03_002132"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SDD03_002132"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG4304568.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="SDD03_002133"
     CDS             complement(2351..3031)
                     /locus_tag="SDD03_002133"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG4304569.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="SDD03_002134"
     CDS             3157..4026
                     /locus_tag="SDD03_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4304570.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="SDD03_002135"
     CDS             4052..4567
                     /locus_tag="SDD03_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304571.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="SDD03_002136"
     CDS             complement(4614..5207)
                     /locus_tag="SDD03_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304572.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="SDD03_002137"
     CDS             complement(5232..5411)
                     /locus_tag="SDD03_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304573.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="SDD03_002138"
     CDS             complement(5488..5997)
                     /locus_tag="SDD03_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304574.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="SDD03_002139"
     CDS             complement(6093..6473)
                     /locus_tag="SDD03_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4304575.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="SDD03_002140"
     CDS             6803..7552
                     /locus_tag="SDD03_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG4304576.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="SDD03_002141"
     CDS             complement(7736..9013)
                     /locus_tag="SDD03_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304577.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SDD03_002142"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SDD03_002142"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG4304578.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="SDD03_002143"
     CDS             complement(9935..10879)
                     /locus_tag="SDD03_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG4304579.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="SDD03_002144"
     CDS             complement(10881..11567)
                     /locus_tag="SDD03_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG4304580.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3319 a         2464 c         2045 g         3798 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaa
//
LOCUS       DARDHU010000081        11552 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000081 DARDHU010000000
VERSION     DARDHU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.082"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(156..701)
                     /locus_tag="SDD03_002145"
     CDS             complement(156..701)
                     /locus_tag="SDD03_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4304581.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(713..1405)
                     /locus_tag="SDD03_002146"
     CDS             complement(713..1405)
                     /locus_tag="SDD03_002146"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG4304582.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1424..1708)
                     /locus_tag="SDD03_002147"
     CDS             complement(1424..1708)
                     /locus_tag="SDD03_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304583.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1721..2284)
                     /locus_tag="SDD03_002148"
     CDS             complement(1721..2284)
                     /locus_tag="SDD03_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4304584.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2512..3174
                     /locus_tag="SDD03_002149"
     CDS             2512..3174
                     /locus_tag="SDD03_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG4304585.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3298..4494
                     /locus_tag="SDD03_002150"
     CDS             3298..4494
                     /locus_tag="SDD03_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG4304586.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4595..5971)
                     /locus_tag="SDD03_002151"
     CDS             complement(4595..5971)
                     /locus_tag="SDD03_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4304587.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6008..6874)
                     /locus_tag="SDD03_002152"
     CDS             complement(6008..6874)
                     /locus_tag="SDD03_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG4304588.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6963..7988)
                     /locus_tag="SDD03_002153"
     CDS             complement(6963..7988)
                     /locus_tag="SDD03_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4304589.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8006..8680)
                     /locus_tag="SDD03_002154"
     CDS             complement(8006..8680)
                     /locus_tag="SDD03_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4304590.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8864..9757)
                     /locus_tag="SDD03_002155"
     CDS             complement(8864..9757)
                     /locus_tag="SDD03_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4304591.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9831..11528)
                     /locus_tag="SDD03_002156"
     CDS             complement(9831..11528)
                     /locus_tag="SDD03_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4304592.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3435 a         2524 c         1979 g         3614 t
ORIGIN      
        1 taacttaagt tgttgataat aaagagattt attaacgtaa aaagaataag tttttttaaa
       61 ataacgtata aaaaagagca taaaacaaag tcgtttgttt tatgctcttc ttttattatt
      121 ttgtgctttt tattttatca aatcactttg tttttttata aaatttctgc gcctagtgtg
      181 gactcaaaat gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggcatct
      241 ttgaaaatat ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag
      301 atatctgtac atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg
      361 tctagatctg ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc
      421 tcttgctcat agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt
      481 cctatcacat tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca
      541 tgcgcatcaa tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca
      601 gtcagctttt tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca
      661 acaaaatcat atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga
      721 gatgtttgac aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt
      781 cgtcaaagct ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa
      841 atgggatatt gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta
      901 atgcttcagg gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat
      961 cacctgtaac gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca
     1021 tttcactgcg caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg
     1081 gtttgtagcc aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga
     1141 tgtcacctac ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt
     1201 tcacgccgta ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac
     1261 cggaacgtac gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg
     1321 cgcgttccca tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca
     1381 ttgcgccgat aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg
     1441 ctaaaaaaac cacacataac attaggataa caataataag agatttcatc aaaaaagagt
     1501 tccatttggt cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt
     1561 tgctttcttt acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg
     1621 cttttcgctg tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc
     1681 ttcgcgtaat aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata
     1741 gttcccagta ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg
     1801 cttgttgcgc ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg
     1861 ctaaaggatt ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg
     1921 caaaacctgg tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag
     1981 tttcttcttc taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga
     2041 tttttccagc tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa
     2101 gaagcagacg atcttgttcg tcaaaagcaa tgatcccgac accaccagga tgaaatacca
     2161 gttctctctt cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa
     2221 tttttccgct atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca
     2281 gcatatcttc tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt
     2341 ctcaaaaact ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa
     2401 atccttcttt agtccgatac tctttctagt agtccgtatg ctaaaataag tatgtaatcg
     2461 aataacgaaa agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa
     2521 cgaaattggc cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt
     2581 ttttcttttg ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt
     2641 ggttcgcgca ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa
     2701 actcactatg aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg
     2761 aatcaaacaa aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt
     2821 aaagcaatcg atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg
     2881 gtgaaagaac caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat
     2941 ctagttgatt tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag
     3001 acctatgaaa aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca
     3061 caagattatc aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt
     3121 gcgaaacaga gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt
     3181 tgttcataaa agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc
     3241 taaagtagag acattcatgt aagaatcgag aacagattca acaaggagga attttttatg
     3301 gatcataaac cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta
     3361 ttgaacaacc cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt
     3421 gatcagctcc aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc
     3481 caagaacaag ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc
     3541 agctacggat cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat
     3601 gtgcaagcac aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc
     3661 caagaagcca atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc
     3721 gggaaggtca aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa
     3781 attgacaaga tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg
     3841 atgttagaac agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc
     3901 gctgctggag aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa
     3961 agagcagaag aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc
     4021 ctagatcgtc tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag
     4081 caagcaccac aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa
     4141 gcatctatcg ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg
     4201 cttcgtcaaa aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta
     4261 ttgaagaaaa attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa
     4321 cgtggaatcg tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc
     4381 caagagacat tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag
     4441 cttggacaca tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc
     4501 tttcgcttgt tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt
     4561 tttcttatct ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc
     4621 catagccata gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc
     4681 atgtcacagt ttgcagacga agcatcaata caatacaagt caagatggag acgattggca
     4741 atagcggaac gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca
     4801 acttcagtaa gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt
     4861 ccgctaatac attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat
     4921 aagtcgcttt tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta
     4981 agccatcctt gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta
     5041 ccgtcagtag cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta
     5101 catacctcat tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc
     5161 ctgtcaagat cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga
     5221 ttcctcttgg cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat
     5281 caaatcccaa gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa
     5341 aaggcatgaa tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca
     5401 acgcgatgat accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg
     5461 cttcaagcga cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt
     5521 atgtaccatt ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac
     5581 ttttcaaaaa accttgcata tagccagacc atccggaagc aacagaggaa accgctagta
     5641 aaaattcccc gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat
     5701 acagataggc atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta
     5761 gtccagacaa aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag
     5821 cattttgatt ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa
     5881 ttccaagcat gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga
     5941 gattgatctc tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa
     6001 acagcgatta aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc
     6061 aaccaacaat aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc
     6121 cacctaaagt agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa
     6181 gtgatttgac tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca
     6241 tgtttttcca cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta
     6301 agatcattcc gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga
     6361 cgatgatggt atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac
     6421 caaaatcaag cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca
     6481 aatacgcacc gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt
     6541 gtgtccattc atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag
     6601 tagacaatgg tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt
     6661 gtccaacact ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta
     6721 caggactaac aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta
     6781 gtgtttgttg attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg
     6841 ccaacatagg gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga
     6901 taagaaactt atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt
     6961 ttctaacgtt tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct
     7021 tttccttcag ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct
     7081 aaataaagtc ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat
     7141 cccttatcaa agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact
     7201 gaaaggctga ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc
     7261 gtatatttga tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg
     7321 gtcaaaacag tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa
     7381 tttgcctgac tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg
     7441 tagtcccgcg agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg
     7501 atttttgata attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc
     7561 gaacgaacta gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca
     7621 atataatctt tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca
     7681 cggatcagct gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc
     7741 aaataatttt gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc
     7801 ttgctcaagt aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa
     7861 taagcaattg tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc
     7921 aatgcaataa aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa
     7981 aaattcatct taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt
     8041 cgatatatcc ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg
     8101 tcaatgtatt gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc
     8161 gacttaagat ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc
     8221 tcaaatcaat gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc
     8281 cattgtggct catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg
     8341 cattgatttt tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc
     8401 ccatgttcat agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg
     8461 gtactttcga tacttcacgt attttttgac accaataata gccatccaaa acgggaagat
     8521 taatatctag taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga
     8581 aatcggtcgt tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc
     8641 gaatgatctc ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc
     8701 ttcttgctac tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg
     8761 tcattttagc aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc
     8821 caagcagctt aaatttatta tgttattctt aataattggt ctattatctg ttatttttct
     8881 ttcgttgttt ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta
     8941 gcaatatacc tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta
     9001 tctttgaaga gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag
     9061 tgttcacggt gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg
     9121 ctaatgtatt tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca
     9181 tccgaccata cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa
     9241 agagtttttt ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc
     9301 cagttggaaa agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg
     9361 tttcatcaaa taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat
     9421 gttgttcgta atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat
     9481 ctttgacttg tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt
     9541 catgcactgc taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat
     9601 aattctcgat ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg
     9661 taaaaactgc agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga
     9721 tttgggtttg aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa
     9781 aaactgcagc aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca
     9841 gacttcattt tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt
     9901 agtaagaatg ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc
     9961 ggtactcttt ctggccctgg attttctact cctacgatct gaatttcttc attatagctt
    10021 ccataagaga tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct
    10081 ggcgccatgg tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat
    10141 ccatttccat ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct
    10201 gctcctgatt cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa
    10261 cggtcttcat tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca
    10321 tatttttcaa actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca
    10381 taatcaaatt catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc
    10441 gcttctagtt ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca
    10501 tctgctaatc tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc
    10561 acgacaaacc cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc
    10621 tcgccggctc gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct
    10681 gtgattttgt aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa
    10741 ggaataggtg ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa
    10801 atcgtttctt gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct
    10861 acattcatct gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta
    10921 ttgtatgtgt ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc
    10981 actggcatca taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt
    11041 tctaagaaaa gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta
    11101 gagaataccg cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat
    11161 tttaaaaacc aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta
    11221 agaccgtctt ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc
    11281 gttacgtcaa acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa
    11341 accaatccgc tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta
    11401 atcgtcactt cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg
    11461 aaaatcccta ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt
    11521 ttattcattt ttttctcccc gttctttttt ta
//
LOCUS       DARDHU010000082        11208 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000082 DARDHU010000000
VERSION     DARDHU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.083"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            170..1429
                     /gene="lysA"
                     /locus_tag="SDD03_002157"
     CDS             170..1429
                     /gene="lysA"
                     /locus_tag="SDD03_002157"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG4304593.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1416..2216
                     /gene="dapF"
                     /locus_tag="SDD03_002158"
     CDS             1416..2216
                     /gene="dapF"
                     /locus_tag="SDD03_002158"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG4304594.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2219..3100
                     /locus_tag="SDD03_002159"
     CDS             2219..3100
                     /locus_tag="SDD03_002159"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG4304595.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3097..3813
                     /locus_tag="SDD03_002160"
     CDS             3097..3813
                     /locus_tag="SDD03_002160"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG4304596.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3819..4514
                     /gene="dapD"
                     /locus_tag="SDD03_002161"
     CDS             3819..4514
                     /gene="dapD"
                     /locus_tag="SDD03_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG4304597.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4530..5531
                     /locus_tag="SDD03_002162"
     CDS             4530..5531
                     /locus_tag="SDD03_002162"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG4304598.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5528..6889
                     /locus_tag="SDD03_002163"
     CDS             5528..6889
                     /locus_tag="SDD03_002163"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG4304599.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6849..8000
                     /locus_tag="SDD03_002164"
     CDS             6849..8000
                     /locus_tag="SDD03_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG4304600.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7975..9144
                     /locus_tag="SDD03_002165"
     CDS             7975..9144
                     /locus_tag="SDD03_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4304601.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9319..10698
                     /locus_tag="SDD03_002166"
     CDS             9319..10698
                     /locus_tag="SDD03_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4304602.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10979..11208
                     /locus_tag="SDD03_002167"
                     /pseudo
     CDS             10979..11208
                     /locus_tag="SDD03_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3730 a         1954 c         2502 g         3022 t
ORIGIN      
        1 tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata
       61 tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca
      121 aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt
      181 gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt
      241 gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa
      301 acgttttgat acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca
      361 tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc
      421 tttgcaagct gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga
      481 ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga
      541 agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg
      601 gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc
      661 aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga
      721 aagcagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt
      781 tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc
      841 tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat
      901 cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg
      961 aaaactgacc attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg
     1021 tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt
     1081 agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga
     1141 tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg
     1201 tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct
     1261 tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc
     1321 gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca
     1381 agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct
     1441 gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt caattagccg
     1501 cttctttatg cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc
     1561 cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca
     1621 ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg
     1681 tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca
     1741 gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc
     1801 caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt
     1861 taggaacgat tcatacagtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa
     1921 gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag
     1981 aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac
     2041 ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt
     2101 cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg
     2161 cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat
     2221 gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta
     2281 tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt
     2341 tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca
     2401 actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa
     2461 tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt
     2521 ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc
     2581 aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat
     2641 gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa
     2701 agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt
     2761 cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg
     2821 tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac
     2881 gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca
     2941 tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt
     3001 accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt
     3061 agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt
     3121 ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt
     3181 ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt
     3241 attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa
     3301 cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa
     3361 catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg
     3421 aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa
     3481 aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca
     3541 atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa
     3601 aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg
     3661 tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt
     3721 gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa
     3781 ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc
     3841 gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc
     3901 caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg
     3961 actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa
     4021 tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc
     4081 gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca
     4141 tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg
     4201 gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg
     4261 tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac
     4321 tggtaggtgc gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg
     4381 cagcaggagc agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg
     4441 cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt
     4501 tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg
     4561 caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag
     4621 gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa
     4681 agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat
     4741 tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat
     4801 ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta atcgttccag
     4861 aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca
     4921 ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt
     4981 attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta
     5041 cgcaaaatga cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg
     5101 aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga
     5161 cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa
     5221 tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag
     5281 aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac
     5341 ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca
     5401 gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca
     5461 aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg
     5521 tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga
     5581 agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt
     5641 tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt
     5701 gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga
     5761 tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc
     5821 acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg
     5881 tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa
     5941 agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat
     6001 cacaaaagca tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc
     6061 tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc
     6121 gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc
     6181 tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag
     6241 agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga
     6301 ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat
     6361 atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt
     6421 tctttccatc actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc
     6481 aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa
     6541 tatcggagtg atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga
     6601 attaaaagaa acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc
     6661 tgtcgttggg ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt
     6721 gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat
     6781 catcggtgta ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt
     6841 gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc
     6901 atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc
     6961 atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg
     7021 cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt
     7081 ctaagcatga aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag
     7141 ggttgactga ctttataact aaacacattg aggatcttat ttataatgta gtactgatct
     7201 atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg
     7261 ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag
     7321 aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta
     7381 aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg
     7441 tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc
     7501 taatgaaatt tggccaaatc caaggtggaa cgatccgcaa tgttttagct ggagaagtta
     7561 ttgtatccgg aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat
     7621 tagaccattt agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg
     7681 gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac
     7741 tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat
     7801 atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt
     7861 tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta
     7921 cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg
     7981 aatttatcca atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa
     8041 ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat
     8101 tttccaactc ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct
     8161 cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa
     8221 cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg
     8281 gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca
     8341 gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg
     8401 atagatactt ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa
     8461 caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg
     8521 acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt
     8581 gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct
     8641 acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact
     8701 ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc
     8761 catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta
     8821 ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat
     8881 ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac
     8941 ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta
     9001 gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt
     9061 tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc
     9121 aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt
     9181 gcggtatatt ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct
     9241 tttgattcat aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata
     9301 ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga
     9361 gggcatcaat cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta
     9421 tttgaagcag gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac
     9481 aaggtaacag atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat
     9541 gttcatattt taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg
     9601 gttactagcg catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt
     9661 tctggtgtca aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg
     9721 atgcgtggat acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa
     9781 aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa
     9841 gctgggaaag aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat
     9901 gctgagatga cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca
     9961 aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa
    10021 aaagaaatcc cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg
    10081 ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa
    10141 acgaatcttc cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca
    10201 cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta
    10261 aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca
    10321 ttaagtgtct ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa
    10381 gcaggagtag atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca
    10441 gaagaacaag gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa
    10501 ttgctaggag gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca
    10561 ctagcgatcc aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag
    10621 ccaggctttg accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa
    10681 gaaccatttg tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt
    10741 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga
    10801 cttattaccg cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca
    10861 cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat
    10921 tttttatggt taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg
    10981 gcaaaggcga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat
    11041 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
    11101 tttctctatc aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac
    11161 ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DARDHU010000083        10922 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000083 DARDHU010000000
VERSION     DARDHU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.084"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SDD03_002168"
     CDS             complement(<1..281)
                     /locus_tag="SDD03_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304603.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="SDD03_002169"
     CDS             423..1541
                     /locus_tag="SDD03_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4304604.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="SDD03_002170"
     CDS             1669..3045
                     /locus_tag="SDD03_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG4304605.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="SDD03_002171"
     CDS             3446..5026
                     /locus_tag="SDD03_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG4304606.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="SDD03_002172"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="SDD03_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="SDD03_002173"
     CDS             complement(5652..5972)
                     /locus_tag="SDD03_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG4304607.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="SDD03_002174"
     CDS             complement(6120..8354)
                     /locus_tag="SDD03_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4304608.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="SDD03_002175"
     CDS             8833..9099
                     /locus_tag="SDD03_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG4304609.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="SDD03_002176"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="SDD03_002176"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG4304610.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DARDHU010000084        10817 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000084 DARDHU010000000
VERSION     DARDHU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.085"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SDD03_002177"
     CDS             complement(25..162)
                     /locus_tag="SDD03_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG4304611.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SDD03_002178"
     CDS             complement(159..1343)
                     /locus_tag="SDD03_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG4304612.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SDD03_002179"
     CDS             complement(1599..1853)
                     /locus_tag="SDD03_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304613.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SDD03_002180"
     CDS             complement(1878..3020)
                     /locus_tag="SDD03_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG4304614.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SDD03_002181"
     CDS             complement(3080..4432)
                     /locus_tag="SDD03_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4304615.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SDD03_002182"
     CDS             complement(4503..4847)
                     /locus_tag="SDD03_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG4304616.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SDD03_002183"
     CDS             complement(4857..5231)
                     /locus_tag="SDD03_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4304617.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SDD03_002184"
     CDS             complement(5244..5558)
                     /locus_tag="SDD03_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4304618.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SDD03_002185"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SDD03_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SDD03_002186"
     CDS             complement(8976..9638)
                     /locus_tag="SDD03_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304619.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="SDD03_002187"
     CDS             10255..10485
                     /locus_tag="SDD03_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304620.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="SDD03_002188"
     CDS             10491..10790
                     /locus_tag="SDD03_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304621.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DARDHU010000085        10724 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000085 DARDHU010000000
VERSION     DARDHU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.086"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(241..1089)
                     /locus_tag="SDD03_002189"
     CDS             complement(241..1089)
                     /locus_tag="SDD03_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4304622.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1088..2155
                     /gene="gnd"
                     /locus_tag="SDD03_002190"
     CDS             1088..2155
                     /gene="gnd"
                     /locus_tag="SDD03_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG4304623.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="SDD03_002191"
     CDS             2157..3158
                     /locus_tag="SDD03_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG4304624.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="SDD03_002192"
     CDS             3155..3439
                     /locus_tag="SDD03_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG4304625.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="SDD03_002193"
     CDS             3472..4269
                     /locus_tag="SDD03_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4304626.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="SDD03_002194"
     CDS             4283..5116
                     /locus_tag="SDD03_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4304627.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="SDD03_002195"
     CDS             5134..5991
                     /locus_tag="SDD03_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4304628.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="SDD03_002196"
     CDS             6013..6522
                     /locus_tag="SDD03_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4304629.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="SDD03_002197"
     CDS             6559..6960
                     /locus_tag="SDD03_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304630.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="SDD03_002198"
     CDS             7072..7425
                     /locus_tag="SDD03_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304631.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="SDD03_002199"
     CDS             7459..7902
                     /locus_tag="SDD03_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4304632.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="SDD03_002200"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="SDD03_002200"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4304633.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="SDD03_002201"
     CDS             8997..9710
                     /locus_tag="SDD03_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4304634.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="SDD03_002202"
     CDS             complement(9917..10066)
                     /locus_tag="SDD03_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304635.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="SDD03_002203"
     CDS             10462..10662
                     /locus_tag="SDD03_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4304636.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1695 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attc
//
LOCUS       DARDHU010000086        10687 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000086 DARDHU010000000
VERSION     DARDHU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.087"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..879)
                     /locus_tag="SDD03_002204"
     CDS             complement(<1..879)
                     /locus_tag="SDD03_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG4304637.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(978..1760)
                     /gene="rpsB"
                     /locus_tag="SDD03_002205"
     CDS             complement(978..1760)
                     /gene="rpsB"
                     /locus_tag="SDD03_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG4304638.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            2119..2598
                     /locus_tag="SDD03_002206"
     CDS             2119..2598
                     /locus_tag="SDD03_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG4304639.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            2815..3906
                     /gene="phnW"
                     /locus_tag="SDD03_002207"
     CDS             2815..3906
                     /gene="phnW"
                     /locus_tag="SDD03_002207"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG4304640.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            3899..4027
                     /locus_tag="SDD03_002208"
     CDS             3899..4027
                     /locus_tag="SDD03_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG4304641.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            4031..4576
                     /locus_tag="SDD03_002209"
     CDS             4031..4576
                     /locus_tag="SDD03_002209"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG4304642.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(5030..6721)
                     /locus_tag="SDD03_002210"
     CDS             complement(5030..6721)
                     /locus_tag="SDD03_002210"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG4304643.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(7141..8088)
                     /gene="arcC"
                     /locus_tag="SDD03_002211"
     CDS             complement(7141..8088)
                     /gene="arcC"
                     /locus_tag="SDD03_002211"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG4304644.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(8203..9222)
                     /gene="argF"
                     /locus_tag="SDD03_002212"
     CDS             complement(8203..9222)
                     /gene="argF"
                     /locus_tag="SDD03_002212"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG4304645.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(9313..10542)
                     /gene="arcA"
                     /locus_tag="SDD03_002213"
     CDS             complement(9313..10542)
                     /gene="arcA"
                     /locus_tag="SDD03_002213"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG4304646.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2980 a         2195 c         1931 g         3581 t
ORIGIN      
        1 ttttttcact tggctcataa cttcttctac aaagttatct tcgcgttttt cgataccttc
       61 gccaacttcg aaacgtacga atgttttaac agttgcgcct tttgatgcaa cgtatttttc
      121 aacagtcata tcaggatctt taacgaatgg ttggtccacc aaagcgattt ctgctttgaa
      181 tttcttcaag cgtccttcta ccattttttc aacgatattc gctggtttgc cttcgttcaa
      241 tgcttgttct gtaagaacag ttttttcatg ttctaattca gcttctggaa tttgtgattc
      301 gtttacataa cgaggattga tagctgctac gtgcatagct acgtctcttg caactgtttc
      361 gtcagttgtt ccatctagaa cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc
      421 accaaatgca gcattgtcgt ctttttctac aacttcaaag cgacggaagc tgattttttc
      481 tccgataact tgagttgctt cgatcaagtc agattcaatt gtacctttat ctgttttgat
      541 tttcattgca gcttccatgt ctgctggttt gttttcagca acaagttctg caatttcttt
      601 tactaaatct tggaacattt cgtttttaga aacaaagtct gtttctgagt ttacttcaac
      661 gattgctgca gtgttacctt tcacagcaac tgaagccaat ccttcagctg caatgcggtc
      721 atttttcttc gcagcctttg ccattccttt ttcacgtaaa agatcaactg ctttttccat
      781 gtcgccttct acttcaacta atgctttttt cgcatccatc attcctacgc cagtcatttc
      841 gcgtagttct tttaccattt tagctgtaac gtctgccatg tttaactcct cctgtgtgtt
      901 tttcttttta aaaaaagctg cttcaaagga gagtgaggct tgtcgcccag cctttgaaac
      961 agctccatca ttcaaaatta ttctgctgaa gaattgtcgc cttcaacaac atctacgatt
     1021 tcttcgattg atgttgctgt gttttcttct gtgaaatctt cttcaactac ttgatcttca
     1081 ccttggtttc cttcgatgaa agcatcagcc atttttgaag taatcaattt aacggcacga
     1141 attgcatcgt cgtttgaagg gattactacg tcgatttcat ctggatcgca gtttgtatca
     1201 accatagcta cgattgggat atttaatttg tgtgcttctt gaacagcaat acgttcttta
     1261 cgtgggtcaa cgatgtacat tacatctggg attcttggca tatcagcgat accgcctaag
     1321 aatttttcaa gacgttcacg ttctttgttc aaaccagcaa cttctttttt aggtagaact
     1381 tcaaaagttc catcttcttc cattgcattg atttgtttca aacggctgat acgtttttgg
     1441 atagtatccc agtttgtcaa tgttccgcct aaccaacgat ggttaacgaa gtattgtcct
     1501 gcacgagttg cttcttcttt gattgcttct tgtgcttgtt tttttgtacc tacgaataat
     1561 gctacgccgc cttcttcagc aacgctcttc atgtaatcat aagctgcatc tactaattta
     1621 actgtttttt gcaagtcaat gatgtagatt ccgtttcttt ctgtgaagat atatttcttc
     1681 atttttggat tccagcgacg agtttggtga ccaaagtgta cgccggcttc tagtaattgt
     1741 ttcatagaaa ttactgccat gtttgtttcc tccgatttgg ttttattttt cctcttctta
     1801 tcttctacct tgccggcaaa ctttttcaag cacctaccga caatccaata agaatgtgga
     1861 tttgctgatg cataaagcac cctcagctat tatacagaca aaagatccgt atcgcaagag
     1921 caaatatact tttctgctca acattcaata gtttacctga aagataaaaa gaattcaaga
     1981 aaaacagtag atttctcttt gtttctttat tatccttttt ccataaaata attttaggta
     2041 taaaagacga atattattgg gcaatccttc ttttttttgt tatgatacat gtgaaatctt
     2101 tcgtaaagga gaaagaaaat gcgcaaagct caaagacatt tgctgattaa acaagtcatt
     2161 gaagaattcc cgatccgtac gcaagaagaa ctattaaaca aacttgggca ttatggcgtt
     2221 accgccacgc aggcaacgat ttctcgagat atccgtgatc taaaaatcgt gaaggcacct
     2281 gatgaaaatg gcgtttcccg cttcgtattg tttcgcgaaa aaggaactcc tgagactagg
     2341 actgaggaag aggatcggct ggttcaaatg attgaagaca ttgtattgaa agtcgaacga
     2401 gtccactttt tgacgatcgt caataccttg ccagacaatg ctcatttatt tgcatctgtt
     2461 ttagatgaga tcaaacctcc ctctattgtc agtacaattg ctggattcga tacgatcatg
     2521 gtcatctcgg aaaatgagga ggacgcaaaa cgagtagaag aatttcttca ccatccaact
     2581 gaagtatcac ttttttaatc agagcacgta ttcctaatat ggaatgcgtg tttttttatt
     2641 ttcaatattt tccttattga ataaaccttt acactcttta caaaaataca tcatttttta
     2701 ttcaaatttt tcaacacctt gattgaatca caataatcat catttatatt ggatgttaac
     2761 aagagcagct tcttgctctt tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat
     2821 aaattattaa cttctggacc attgacgact tctgaaacag tgaaaaatgc tatgctgatc
     2881 gatcattgta cttgggatca agaatacaaa gaaattacac aatggataag aaaaaaatta
     2941 ttggatcttg ctcaagtatc ggaaggaata tacacaacgg tcttaatgca aggtagcagc
     3001 tccttcgggg tagaatcggt attaagttct gtgatcaggc cagaagatac tgtactgatt
     3061 tgttcaaacg gcgcatatag acaacgaatg atcaacatgt gcgaatgtct gaaactgaac
     3121 tataccgttt atgaggtatc ggaacatcaa atcctccaag ctcatgtgat tacacagctg
     3181 attgaagaga atccgtctat ctccgcacta gcaatggtcc atagcgagac tacttccggt
     3241 attttgaata atttggaaga gattgctgct gtcacacaag aaagagatct tttgtttata
     3301 gtggacgcga tgtcaagctt tggtgggata ccgatttgtg tagccgaact agggattgat
     3361 tttttgattt ccagctcaaa caaatgtatt caaggtgttc caggcttttc ttttgtactt
     3421 gctaataaag cggaactgga aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta
     3481 tatgaccagt accgcgtgat ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc
     3541 catacggttt tggcttttca acaagcgctg aaggaactag aggcagaagg aggaatcgaa
     3601 actcgatatt cacgttatct cgccaataat catgtgctac gacaaaaaat gacagagatg
     3661 gggttccagc ctttcattca agaatatcag gggccattca tcacctcttt tctttaccct
     3721 aaggactggc gatttgattt taatcatttt tatcagtatt taaagcaaaa tggatttgtg
     3781 atctatcccg ggaaaatcag cgatcatgaa tgttttcgta tcggaaatat tggcgagatc
     3841 tatccggaag atattttgga actgacagca attatcaaag actataagga gaacgttaat
     3901 gattaaaact gtaatttttg attgggcagg aacaactgtg gactttggct gtatggctcc
     3961 tgtccacgct tttagaaatg cttttctaga aaaaggaatc caactgactg acaaagaaat
     4021 ccgctaaccg atgggaaaat taaaatggga tcatatccaa cagcttcttt ccctcccttc
     4081 tgtcaaaaaa caatggatag ctatttatgg acaactacct caggaaaaag aggtggagga
     4141 attgtatcaa caatttgaac attatctgtt taaagatttg gtcaagcatt ctaccttgaa
     4201 acccgacaca ttggaaacga tcgaacggtt gagaaacgct gggatcaata ttggttccac
     4261 gaatggattt aatgctccta tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta
     4321 ttcaccagac ttcatggcaa cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta
     4381 tatgatccat aaaaacatgc agtacttcaa aaacaagtcg gttgatgaag taatcaaagt
     4441 cggagatacg gtctcagata tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt
     4501 aattgaaggt agttcgctaa tggggatttc ttatgatgag taccaaaaat tagagatggg
     4561 gaaaaagatc gtttaaaaca gtctgttgct cttagattcc aacaagccgg cgctgatatt
     4621 tgtgtaaaca atttaaaaga gttaacagaa tacattttgt acgctgacat gaatccagaa
     4681 tgacttttat acaaaataag aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca
     4741 gccaagcaaa aatagctcct catatttttc gtttggttag gcttattacc gcagatgcaa
     4801 gcttttgaac accgtttatt cgaaaaaaaa aagggagctg tgacacgact tttgtcacag
     4861 tccccttatt tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa cggtgttcat
     4921 tcagctgact ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt
     4981 tgtttctcta atactcggat cagaacgctg atttcacatt ctctttttct tacatcttat
     5041 ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa taatacagtc actgcataaa
     5101 ctagagctaa gcgtgcttct ttttgttcat catcagctaa aatcttcgta tgagcatagt
     5161 atttgttgaa tgcttgtgct aatttgattg catgtttcgc aatcactgac ggttcgtatt
     5221 tttccccagc agacaagacc gtttcagggt atttttgaac taatttaacc acttcccagc
     5281 tggtatcgtc attcaatgca tagtctgctg tttctgaagg gacaaatcct gctttttcca
     5341 acaggctgac tgcacgagca tgtgtatatt gcacataagg tccagtttct ccttcaaatc
     5401 gaacaacttc ttccaggtta aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga
     5461 aaatgactgc cccaacccca acttgtttag ctacagcatc tttgttttcc aaatcagggt
     5521 ttttctcgct gatttgttct ttagcagatt ggattgcttc atttaatact tcttctaata
     5581 agacgatctt tcctttacga gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg
     5641 gaatatggtg catgtcgtca gaccagtcaa agcccatttc cttcaagaca gctttcaact
     5701 gtttaaaatg atagctttgt tcattcccta ctacatatag agattgtttg aagtcgtaag
     5761 tacgtttacg gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg
     5821 acttcttaat caacgctgga ttcaggtcat aagcagatag gtcaacgatc tctgcacctt
     5881 tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac gatttcatcc atcttgtcat
     5941 tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc cagcagatca tagattttat
     6001 tgaattcttt catcgattca tcacggaacc attgccataa ttgaatagcc tctttatcgc
     6061 cttcttccag acgtttgaac caagcacgag cttcttcatt taattctggt tctgtttcag
     6121 ctacttcatg gaattggacg tataaacgaa gcaattcatt gattggttct gctttaacag
     6181 cttcttctgt accccatttt ttgtaagcga cgatcagttt tccaaactgt gttccccagt
     6241 cacctaaatg gttgatgcgg attggctgat aaccgatttt ttccataatg aaaccgatcg
     6301 aattaccgat aactgtcgaa cgcaaatgtc ccattgagat tggtttggca atattaggag
     6361 aagacatgtc gattggtact gttccttgat tcccgatgtt gctatcccca taatgttctt
     6421 tttctttcac tactgtttgc aaaacttttt tgctgataag ttctttattc atgaaaaagt
     6481 tcaagtaagg tccgacaact tcgatttttt caaagttggc agaatcgatt ttttctgcta
     6541 aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa
     6601 atgcaacatc tccgtgttca gctgattttg gattttctag taattgctcg acttgttcca
     6661 gtgttaaatc atctttgaca gcgtcataga cagcttttgc gacaagatct ttattattca
     6721 tctttattcc tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc
     6781 atacttagta tgccatcact agagacgaga tttcccgcgg taccactcca attgtcctaa
     6841 aaaaggacca gctccatctg atatcggtag acgaccgtct gtttaaaaca gaattctcca
     6901 agtgcgcttc gttgtatctt cttcattcgg cttccaccac ttccgaattc gctagccaag
     6961 aatgatacaa gtactctctt gttcaacaaa tcttagtctt attaaacttt cttgaattat
     7021 atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa acaatccaga tggcctaaag
     7081 gagaagaaat accacctgga tagctataaa tcatttcgta aatacgaaag acttaacaaa
     7141 ttaatcagct acgataatcg ttccttcttc agaagctaaa agattctcga tattttctaa
     7201 agaagtgatg attgccttag cgtttggttt tgcttcaaca aatgcaatag ctgcttctac
     7261 tttcggaagc atgcttcctg gagcgaattg cttttcatca atgtattgtt tcatttctga
     7321 aacagtaaca gtttctaatt tcttttgatc tggtttttgg taattgacat acacattgtc
     7381 tactcctgtc aatacgatca gcaaatccgc atcgatgatt tcggctaatt tttctgaagc
     7441 aaaatcttta tcgattacgg cttctcgtcc ttctaatcca gtagctgttt ctacaacagg
     7501 aatcccaccg ccaccaacag agactgtgat aactccttga tcgaccagcg tttcgatgac
     7561 acgtgcttct ttaatcgaga tgggtttagg ggaagcgact acttttctcc atccacgtcc
     7621 agcatcttct ttgaatgtca cagtggagtc agcattcatt tgttctttgg cttcttcttc
     7681 cgtgtagaat ggaccgactg gtttacttgg atttttgaat gaaggatcat tttcatccac
     7741 aataacttga gtgactaaag aaaccacatc tttatcgatc cctttttcct tcagtacttc
     7801 gcccatggcg ttttgtaacc agtaaccgat agatccttct gtcattgcaa cacatgtatc
     7861 taaaggcatt gccggtgttt tctcagaatc agcagcttgc tgctgaatca ataggttacc
     7921 gacttgcgga ccatttccgt gggagatgat caattcatct ccttgttcga taaatttcac
     7981 taaatacttt gctgtttcca ttaacgcttc ttgttgggct tttgcacttg catccgtaga
     8041 caagatggca ttgcccccta aagcgacaac tacttttcgt ttggccattt cattcgtcct
     8101 ttcttcagtt ctaccgaatt ttctagataa caaaacgagc cagcgttttg tgattttccc
     8161 tcaatgctga ctcgtttttt cgtgagatga atgtggttat gcttaaacgc gagggataaa
     8221 caagttacct aatgttgctg ccatgatcgc tttgattgag tgcatacgat tttctgcttg
     8281 gtcaaattga cgagcatatt tgctgcggaa gacttcatct gttacttcca tttcagtgat
     8341 gccaaagcgt tctttcattt gttcaccgta aacagtgttt gtatcatgga aagctggcaa
     8401 gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt tctaccattt ccatattgat
     8461 ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta tcttcttcac ccattgatac
     8521 ccatacatca gaataaagga cgtctgctcc gtctacacct ttatcaacat cgtcggtaat
     8581 catcaattgt gcaccggatt tttcagcaaa gccttctgcc attttgacga tttcttcttc
     8641 aggttgtaat tcttttggtg caacgatacg catattcgct cctaaaattg cacctgtgac
     8701 taataaagag ttagccatat tgtttcgacc atctccgcag taagcaacag tgataccttc
     8761 tactgttcca aagttttctt ggatagttaa aaagtcagcg atcatttgtg ttggatgcca
     8821 ttcatcagtc aagccattcc aaactggaac accagagaat tctgctagtt cctctaccat
     8881 tttttgactg aatccacgga attcgattcc gtcaaacata cgtcctagta ctttagctgt
     8941 atcttctgtg gattcttttt tacctaattg gatatcattt gcacctagat attcaggatg
     9001 tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag atgttttttc
     9061 aaataaaagc gcaatatttt ttccttctag atagtgatgt gggataccac gttttttcaa
     9121 gtctttcaag tgttctgaaa aatcgatcaa atattgtaat tcttcttttg taaagtcttt
     9181 ttctgctaat aagcttcttc cttggaatac tgattctttc atattaaaca ttctccttta
     9241 gattgttttt atttaagatt aaagaggaaa tcccgacggc agctgacagt aagactgccg
     9301 tcaggaaaca aattacttca agtcttcacg gacaagtggt tggctcatgc agcgtggacc
     9361 accacgacct cgtgacaatt cacttgaatt gatttctaac actttgatgc catagctacg
     9421 aagcaattcg ttcgatacat aatttcggtt ataagtgact acaacacctg gagcgattgc
     9481 caatgtgttt gatccatcat tccattgttc tcgaggtgca acgatctcat ctccattacc
     9541 agttgggatc agtacaagat catctaatcc aagagcagct ttcatcgttt cgtgtaagtt
     9601 atctgaatga gtaatcttga tatcatcacc atctggttcg attgtaaaga catccatctt
     9661 accgtcttta ctttgaattg ctggatggat agtgaattta tcgtgatcta ccattgtaaa
     9721 tacggtatcc aaatgcatca ttgcacggtt gttcggaatt ttgattgcca acactttttc
     9781 aaatcctgaa ttttttgcga ataggtggcg tgctaatttt tctaaagctt tcgcattagt
     9841 tcgttgagaa atcccaacag ctactacttt atcgcttaag atcaattcgt ctccgccttc
     9901 gatatgatct ggattttcac gatccaacca tacttcgatt cctttatttg caaatcttgg
     9961 atgatgtttc aaaatatatt ctgtaaacat cgattcacga cgacgtgctt caaaagtcat
    10021 cttattgact gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt
    10081 tggcattggg tccatgtaaa atggatagtc atcgtctgct gacagatcat atagactgct
    10141 agaacgtact tcgatatctt ttgtacgaac cccagccatg attttatcta ccatttcttg
    10201 cgtttccatt gatagaagga actcatgaag accctctctt acagcattcg atgcaatatg
    10261 agattcatcc aacattttat tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc
    10321 tgccgctaat ttttccaagt aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa
    10381 gttgtcatgt tcttcttgag caatcggaag atatggaata tcatcgaaca acagacgatc
    10441 cataatatct ggtgtcaaat tttccacttc ttgtccaggc cgtttcagta aaactgtctt
    10501 taatttccct atttcagaga aaacgtgaat aggtttatcc atgttgtgtt gcctccttct
    10561 ttttgattac atgtatagga tatcctcctt ttgtaatcac tttcataatt ttgtctcatt
    10621 caaaatgaga cattcctcac attaatattc gataattaaa taattaaacg tttttctatt
    10681 tttaagc
//
LOCUS       DARDHU010000087        10264 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000087 DARDHU010000000
VERSION     DARDHU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.088"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            298..1146
                     /locus_tag="SDD03_002214"
     CDS             298..1146
                     /locus_tag="SDD03_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG4304647.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1291..2232
                     /locus_tag="SDD03_002215"
     CDS             1291..2232
                     /locus_tag="SDD03_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG4304648.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2222..4393
                     /locus_tag="SDD03_002216"
                     /pseudo
     CDS             2222..4393
                     /locus_tag="SDD03_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4505..6676)
                     /gene="copB"
                     /locus_tag="SDD03_002217"
     CDS             complement(4505..6676)
                     /gene="copB"
                     /locus_tag="SDD03_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG4304649.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(6696..8882)
                     /locus_tag="SDD03_002218"
     CDS             complement(6696..8882)
                     /locus_tag="SDD03_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG4304650.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(8882..9091)
                     /gene="copZ"
                     /locus_tag="SDD03_002219"
     CDS             complement(8882..9091)
                     /gene="copZ"
                     /locus_tag="SDD03_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG4304651.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9104..9544)
                     /locus_tag="SDD03_002220"
     CDS             complement(9104..9544)
                     /locus_tag="SDD03_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG4304652.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9618..10157)
                     /locus_tag="SDD03_002221"
     CDS             complement(9618..10157)
                     /locus_tag="SDD03_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4304653.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         2900 a         2175 c         1830 g         3359 t
ORIGIN      
        1 taaaaagata aaaaactccc tttctattta tttaaatacg caaatcttcc tcattatttt
       61 ttattttact caatttaatc gtattctcat ttcaaaacga atgtacttta aaaaaacata
      121 ataagatcac tttgcttgta tgatgtattc ccttccatta caatgaaata aatagcgatt
      181 ttatgagaag gtgatgtgaa aatggaagaa aaaatagaag aatccaaaca aaaaatcgat
      241 cagttgatat aagaattaga aaaagcggtc atcagcaaac gttcaactat ccaattgatg
      301 atcgtggccc tattagcagg tggtcatgtc ttattcgaag atattcccgg tgtaggtaaa
      361 acgttactgg tcaaagcact agcaaaagct gtacagggaa ccttcagcag agtccaatgt
      421 actcctgatc tgctgcctag tgatattctt ggtttttccg tatacaattc acaatcaaaa
      481 gagtttgaat ttcgtccggg accgatcttt accacgatct tgttagcaga tgagatcaat
      541 cgtacgactc ctcgtactca gtcagcactt ttagaagcaa tggccgagaa tcatgcaacg
      601 atcgacaaca atacataccc tctagacaat catttttttg tcttagctac gcaaaatcca
      661 atcgagtacg aaggaacata tcctttgccg gaagcacagc tcgatcgttt tttattccga
      721 ctacaaatcg gttatccttc ttttgatgac gaacttttgc tacttttgga taaattcgaa
      781 aaagtgttgg ataacttaaa tgaagtattg aacagttatg aaatagaaga aataaaaaca
      841 aatgtggcga atgtctttgt cagtcccgaa gtggcatctt atgccttgca gttagtgtca
      901 gcaacaagaa gtcatgctgc catccagctt ggcatcagtc cacgaggaag cctttccttt
      961 attcaggcag caaaagctta tgctttaatc catgggagaa attacgtcac gcccaaagat
     1021 ttgcaggatt tagttcctta cgtgttttcc catcgcctga ttttttacga tcgttcattg
     1081 aaggaagacg aaaagaaaca aatcattcat actcttgttg cacaaattcc tattcctgtg
     1141 aggtagattg aggtagacaa tggaaaaaat caaagattat ttcattaatt ttgtgttatt
     1201 tctggtatat ttcatcattg tgctttttgc tcttgtattc aacaattctt taggctggtt
     1261 cttgttggcc ttttttactc tttatgtatc atggagcttg atttcattaa gcagctttct
     1321 aaagaaactt tcggttactt gtacattgca gccactcgtt tatcgcaaaa aaagtatgca
     1381 ggttattttt tccatagcaa aaaagagaac tttttcttta cctctccctc gtttacaaat
     1441 cggcttccca gcaatctttg cagaaaaaaa caagagattt tgattcttac aaataaaccg
     1501 caagaagtcc aattgctttg gcagccaaaa gaaagaggat gccatgaaac attaactgtc
     1561 atttttacag cctatgatag tctaggactt ttcagaaaaa gtgcaaaaag agagatctct
     1621 tttcctgtga ctgttctgcc tgctttttgt aagattcaag cagaaaaatt acagagagtt
     1681 ctggaaaaaa aacagcagct gaacgcgtac caaagaggtc ttgattttcg ggaacatcgc
     1741 gcttatcgtc caggtgattc tttcaaccga atcgattgga aattgtctgc tcatactcaa
     1801 gagtggttat accgtgaata cgattatcaa gaagaagaat gttctcctct tttttgcttt
     1861 tggggtttgc ccagtccaaa atttgaacct ctgctagact tgtatttttc tttatggcat
     1921 ttgagaaaag aaaagcagcc ggaacttctg atattaggca accgcgcctt tcatggcaaa
     1981 gaccccggtc atacctattt tgcttctttg gaagctggcg atgagaaagc ttttttcgca
     2041 cacttgaaaa aatatgcaaa aaaacaaatc gtcctctttg ttcctgagaa ttctcctgaa
     2101 gtctcagaag ctattgaaac attgtcaaaa gatcgaacgg tttatcttgt ttatttcgta
     2161 gaaaaacaac tcatggtcca agaaaaagac aagatatgtt cattgccagg aggtgaatgg
     2221 attgacgatt aaacgggaaa aatggctgcg tggcgtttac tcctttgtga tactaagtgt
     2281 aggtgctccg atatttgtag tagctaatca attgcaaaac agctaccttc ttatcctaac
     2341 tattggtgtc ctatgcctta ttgtctgctg tattcctaaa aaatgggtac gtttgctgct
     2401 ttctttgcca gtaatgatag gttgtttgtt tttatatttt ccttcgaaga cattttccat
     2461 attatggatc ttacagtttt tttcgtcagt cacagaggaa ttcttccatc tatcccaagg
     2521 taaactttat tatttgccag aaaagacagc tttttttctc ataatgttgc tgatctattt
     2581 atttattaca ttgaccgtgg attacgacta ttggtacgca ccctttcttt ctttgatggc
     2641 ttacttcatg atgcttaccg tttttcggaa aaaagattta ctgtacgaaa tgattgcggt
     2701 agtaactgtt ttgttcgtgt atctggcagc aaggcaattt ttctccatcc ggttgttgtc
     2761 gggaaacact cgattccagt cattgactac agttctgctt atttgtttct taacttttag
     2821 cgctatcttg cctttatatc ttccgaaagt acaccaatct ctcgaggaaa cgagtgaacc
     2881 gatccgagaa tatcttaatg atgaagggct ttatgacaca ctccatgagt atcaattaac
     2941 cggaagtgca agaacaggct tcagtgagaa tgatgaacag cttggtggac ctttatatga
     3001 taatggcgaa gttgttttta cagcgaacca aaaaggaaat ggctattgga agatcgaaaa
     3061 taaaaatcat tacaccggtt ctggatggat agaagatcag gacgcaccca tccaaccaat
     3121 ccaagaattc cctgttgaca tcacgagcta ccaaaccgtt atagaagaca aagaaaacat
     3181 tacattgacc ctaaaaaaac aagttttctt accttatcct tatggaaata ctaccttgac
     3241 tgatttttca ttagctagtg gacaaacgct ttactatttg cctgaaagcc agcgattcat
     3301 tcctgtcccg aatgaatcga cacgttctgc aactttacaa acagaaaggt tagtttttac
     3361 gagagaagaa ttaaaaagca gtcagttaaa agctgatgat ttagaaaatc agcagacaaa
     3421 tcttcaattg ccaaaaagtt tgcctaaacg tattgaggac ttggcggtcg aattgaccaa
     3481 aaatgaagca actttaattc gtaaagtcga agctgtctaa cagtatttaa aaacaagcgg
     3541 aaatttccgc tattccaaaa cggataccgg gcatactcca aaagaacacg attatgtgga
     3601 ttatttcctc tttgattcaa aagtcggcta ttgtgacaac ttttcaactt ctatggtcgt
     3661 ccttcttcga acacttggta tcccagctcg ctggacaaaa ggttttacaa atgggctcag
     3721 aacaggcaca gataatcaag ggaatgagga atacagcatt ctcaatagtc atgctcattc
     3781 ttggccagag gtatactttc ctggatttgg ctggcttcca tttgaaccaa ccccttcatt
     3841 tgccaatcca atgggttcac aaccagttgc tgaaacaacc gaaaccgtcc agcctgctat
     3901 cgaacaacca aatgaaagcg aaaatatggc agaaactatt tccagtgaga gatctgaggt
     3961 cattccatca gaagaaacaa cactcacacc agcttataaa atcgtcttat tcagccttgg
     4021 agtgtgttta tccgtcttgt tgttctttct attgttattc agaaaatctc tgcgctggac
     4081 aagttatctt atctatcttc atttttttgc ttctactaat acgattgcat atcaaaaact
     4141 tcttaaccct ttggaaacat tggtaccgcg caaacccgca gaatctttgc aggaatatag
     4201 cctgcgaata gaacaagaat tcaagctaga tctgcgtttc attcgattaa ctactcaata
     4261 tgaagcagaa atatatggac gacaagcaga tccggtaaaa ttcgataaaa aaacctattt
     4321 agaaataacg catataataa gaaaaatggt atacaaaaaa attctgacaa aagggagccg
     4381 cttcaaaaaa taagataaaa tttctggaga atctacttct ggaatgccga gaaccagctg
     4441 attgagtatc gattactcga aaaaaaggtt gtgacacgtt ttttgtcaca accttttttc
     4501 attattatct gattttcaat gtcaaagcat ttagagctac aactaccgta cttaacgaca
     4561 tcaagacagc accaactgct ggacttaata tgataccaat tggcgcaaga attccggctg
     4621 ctagcggaat tgcaagaata ttgtaacctg ccccccacca gaggttctgg atcattttct
     4681 ttcgcgtttg cttggctaga tcaagaaaat gcaaaatatc ttttggatcg ctgtttgtca
     4741 atacgacatc cgcagaatcg attgcgatat ctgtccctgc accgattgcc atcccgattg
     4801 aagcacgggc taaacttggc gcatcattga tcccatcacc caccatgatt acttttttcc
     4861 cttggtcaag gtatttctgg acaacagctt ctttatcatc tggtaataaa ccgccataat
     4921 attcttcgat ccctaaataa tctgctactg cttgggcagc gtttttattg tcaccagtaa
     4981 gcatgacagg agtgattttt ctttctttca atgttcggat aaattctttt gcttctgttt
     5041 ttactacgtc acctaaagca acgattgcag ctagatgatt ttctaaaatc aggaagctga
     5101 tggtatttcc ttgttcttgg taaggtttca ataatgcttg ttctacgttt aatccgagac
     5161 gctcaacttc tttttcatta actattttca cttgttgttt tttgactgtt gcctctagcc
     5221 cgactcctga aagatttttt agattttcag cttgataagg cttgatttca tgttctttca
     5281 aataattcat aatgccaaca gccaatggat gattggcatt cgcttccaag gctcctagat
     5341 attgaaggat ttcattttta ttgaacgcat catcaagtac ttcgacaccc gttacagtaa
     5401 attttccttc tgttagtgtt cctgttttgt ccagcatgat cacatctaga tcattggctt
     5461 gctccagtgc attgcggttt ttcaaaagta atccgttttt agctgctatt gatgtggaac
     5521 gagcaacaac taatgggata gctaaaccta gagcatgagg acaggcgatg ataaagactg
     5581 ttaccatacg ttctaatgca ttaggaagat ctgtcaagaa caaccaggcg atgaatgcca
     5641 ggatacccac aattaatgcg acataaaaca gccattttgc cactttgtca gataatgact
     5701 ctagcttgga tttttctcct tgtgcttttc gcaccatttc cataaccttc gctagataac
     5761 cattttcacc agttccagtt acttcgattt cgatcgttcc atccccattg atagaaccgc
     5821 caatcacttt gtcattggct tgttttttga ttcctcttga ttcgccagtc acagctgatt
     5881 cgtcaacaat tgtttcacct ttcaagatta caccatctgt tggcatttta tcaccagacc
     5941 ggacgatcaa acgatcacct tcgtttactt ctttcaatga aactgtctct tcttttccat
     6001 ctttcgtcaa gcgtttcact gattctggca gtaattctgc tagtttttgt aaagcattgc
     6061 tggcattcga cacagcattc atttcaatcc agtgacctag tagcataata acgatcaaag
     6121 tagccaattc ccagaagaaa tccatgacgt gtgtatgtgg attgagcaga tttgcgataa
     6181 acgaatagac actataaata tatgcaactg taatccccat cgcgatcagt gtcatcatag
     6241 caggactttt ttgtttcaat tccatcttag ctccacttaa aaatggctga ccgccataga
     6301 taaatagtat agttgctaag atcaatacga gccaatcaga accaggaaac gttacttgaa
     6361 atggaaattc cattcccatc attggtgaaa aaagaatgat tgggatagct aggataagcg
     6421 atagccaaaa tttttgtttg aaattcccca tgtgcatgga atgatccatg ccgccgtgtt
     6481 cgtgatccat gtgcatcgaa tggtccattt gtttgctgtt tgctatatct ccatggttat
     6541 gtcccatgtg catggaatga tccatctgac tatggtcatg tttcatctcc atctgttttt
     6601 catgagattc tgtactttct ttgtttacta cttctttttt atgctcatgt tttttctcca
     6661 tatcgttccc attcattttt actccatccc ttctcttact ttttcatgtg tcgatttaag
     6721 cacagggagt tcaacaatac actgacggaa ctaaacgcca tcgctcctcc tgcaatgatt
     6781 ggattcaaaa agcctagtgc ggcaaaagga ataccaatcg tattatagac aaaagcccag
     6841 aaaagatttt gtttgatttt tctcaatgtc aacctagaca actcgattgt ttgtgcaatg
     6901 gatgccaaac tgctattcat cagcgtcaca tcggctgttt ccatcgcaat gtccgttcca
     6961 cttcccattg ccataccgac atcagctaat gctaaggccg gtgcatcatt gattccatcg
     7021 cctgccatac cgactttttt gccatctttc tgaagttttt ctacgtaggc tgctttatcc
     7081 tctggaagta cttcagcaaa gatatgcttt ggatcaatac caacttgttt accaatcgtt
     7141 tcggctgcca gtttgttgtc tcctgtcagc ataaaaacat ctaatccttt attctggagc
     7201 tgcttgattg cttgtttgac ttctagtttg atttgatcag ccacggcaat caacccgatc
     7261 acttgctttt catcagctaa aaacatgact gtttttccct gctgttctaa ttctaacgcg
     7321 tgttcttggt attcatcaaa tgataggttc aattcaatta atcgtttacg tgtacccgca
     7381 aaatagcgta ctccgttaat cgtaccgctg attccagcac ctgggtgggc tgtaaaatca
     7441 atcatatcgt atgttgctgc ttcttgcagt tttccgtatt ccacgattgc tttacctagt
     7501 ggatgttcag aagcttgttc taaagtataa aaaatagata ggacttcttt agaaccgact
     7561 agatctgtaa ctttgggctt tccttctgta atcgttccag ttttatccaa aacaatgctg
     7621 tctaagtgag cagctgcttc taatgcttct ccgcctttaa ttaaaattcc atttcgtgca
     7681 ccaacacccg ttcctaccat aatagctgtt ggagtcgcta aacctaatgc acatggacac
     7741 gcaatgacta atacagagac actatggatc aatgcgagtt gccaatcacc agtgatcaaa
     7801 cctgtagcta tgagcgtgat aaacgcgatg actaatacga tcggaacaaa aatccctgag
     7861 atccgatctg cgattttttg gattggggct ttgctccctt gagcatcttc caccatttga
     7921 atgatttgcg ctaatactgt ctgcttgccg acttgtgtaa cttccatgtg aagtagacca
     7981 ttcgtattga ttgtcccgcc aaagagcgtg tcatccggat tcttttctac aggaagattt
     8041 tcaccagtaa gcatactttc atctatcgca ctgcttcctg aaataattcg accatctgct
     8101 ggaatctgct caccaggacg gatgactagt tgatcaccaa cgactacttc ttcaatcgct
     8161 aacgtttctt ctttcccgtt tcttatcact tgggcggttt ttgtttgaag agacatcaat
     8221 tgtttgattg cattacctgt tttcgttttt gctgtatgtt ccaaatattt gccgagcaaa
     8281 atcagcgtga tgatcatact gctgctttca aaataaagat cttgggggtg tccacggaaa
     8341 aatccgttgt aaatgctcaa tgcaaaggca gccgatgttc caattgctac caatacatcc
     8401 atatttggtg ctttcgtctt gattgcatgg tacgcacctt tataaaaacg agcaccgaca
     8461 taaaattgga caggagcaga cagcactaac tggacgattg gaaaatgaaa aaagtgaaca
     8521 atcgccgcat ggcttcctaa catcatagca atcattgaca acatcaacgg caaagtcaaa
     8581 accgtactca atataagatc gaatagcatt tttttcaaat acgcttgctt ttcttctgca
     8641 attttttgtt tgtgggcttc atcgtacaaa attgcaccat agccgatgtt ttctacgctt
     8701 tggattaatt tttcagtggt cgtcccttca tatttgacac ttgctttttc agtggccaag
     8761 ttgactgtgg cattgaccac accaggttgt ccgttcagct ctttttcgat cctagcagaa
     8821 caatttgcac aagtcatacc tgtaatgacg aacgtatcca ttttggtttc tgctgttgcc
     8881 atcaaatcac ctctgcttta taacccaatc cattgattgc ttgacaaatc ttttcggatt
     8941 gaacttttgt ttcatcaaac ttgaccgtac cgtttgcttt tttcaaattt actttcactt
     9001 tttgcacgcc gtcaagtgcg cttactgttt cttctactct tgctgcacaa tgattgcagc
     9061 tcataccttc gattgaaaat tgttgtttca ttttttcttc ctcctattga tgttgtttac
     9121 attcacattg acctgggata cagttacatt cgatcgtatc tactggttgc ttttgttcca
     9181 gttgtttcaa aatctcattg atatcttctt gagtcaaagt ggcttcttct actagatccg
     9241 caatcgtctc tccaatcctt tttgcacaga tatgggaaaa aagattttcc gtcgcactgc
     9301 gaactgtttc catctccgag acagctggat aataaatgaa tttttttcct tcttgttctg
     9361 tccagatcac ttcttttttt accaaacgac ccagcaaagt tttgatcgtt gctggtttcc
     9421 aatccatcga atcagctaag atctgcgtga tttcttgggc tgtcgtctgt ccaagtgtcc
     9481 aaataacacg catgatctcc cactctgaat cactgatctt tactggcgtt ttgacttttt
     9541 ccatttattt cacacctttc gtttacaaat gtaatcatta tcttatgaat acagattaca
     9601 cttgtaaaca aaaactgtca attatttttg ctctctattt tcaatttagt taaaaattct
     9661 tgtttctctc gtcttgtttt aaggatgatc agtttatttc ttgcattagc tacttggatc
     9721 agctgctgta ttttcggttc attgttttct tcaaaatgcc agataaaaga taaaaattct
     9781 aagtattccc tgtttagttt ctccgaaaat gattcaggca tatctggccg agattttttg
     9841 tttatctttc tttttagaga acgccaaact actcggtaga tggcgagata acgcggcact
     9901 ttaagccaaa tgatcttatc tgcttcttta atcctttcct ctaatgtaga agtataattt
     9961 ccttcaacga tccatgacgg attagatacc ataaacaatt gctgttcttg caaaaaccac
    10021 ttttttgcct gaatactgta gtctgttgta tgccataaaa ggtctagcgg cagtagcgga
    10081 aagtcggtga tttctttgat cttccttgcg agggtcgatt ttcccgaccc actttgtcca
    10141 atcacgatga tcttcattct tctccctcac ttctctttgg aatttggttc gatcatacca
    10201 cttataacgc cgcaaaaaaa caagggactg tgacaaaagt cgtgtcaaag tcccttgttt
    10261 cgaa
//
LOCUS       DARDHU010000088        10193 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000088 DARDHU010000000
VERSION     DARDHU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.089"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            120..626
                     /locus_tag="SDD03_002222"
     CDS             120..626
                     /locus_tag="SDD03_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG4304654.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="SDD03_002223"
     CDS             643..1542
                     /locus_tag="SDD03_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG4304655.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="SDD03_002224"
     CDS             1629..2513
                     /locus_tag="SDD03_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG4304656.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="SDD03_002225"
     tRNA            complement(2705..2776)
                     /locus_tag="SDD03_002225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="SDD03_002226"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="SDD03_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG4304657.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="SDD03_002227"
     CDS             4319..5410
                     /locus_tag="SDD03_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG4304658.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="SDD03_002228"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="SDD03_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG4304659.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="SDD03_002229"
     CDS             6562..7752
                     /locus_tag="SDD03_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG4304660.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="SDD03_002230"
     CDS             7856..8611
                     /locus_tag="SDD03_002230"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG4304661.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="SDD03_002231"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="SDD03_002231"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG4304662.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DARDHU010000089        10086 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000089 DARDHU010000000
VERSION     DARDHU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.090"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(247..759)
                     /locus_tag="SDD03_002232"
     CDS             complement(247..759)
                     /locus_tag="SDD03_002232"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG4304663.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SDD03_002233"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SDD03_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG4304664.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="SDD03_002234"
     CDS             complement(3212..3655)
                     /locus_tag="SDD03_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG4304665.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="SDD03_002235"
     CDS             complement(3656..4444)
                     /locus_tag="SDD03_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4304666.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="SDD03_002236"
     CDS             complement(4441..5382)
                     /locus_tag="SDD03_002236"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG4304667.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="SDD03_002237"
     CDS             complement(5559..6146)
                     /locus_tag="SDD03_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG4304668.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SDD03_002238"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SDD03_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG4304669.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="SDD03_002239"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="SDD03_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG4304670.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="SDD03_002240"
     CDS             complement(9063..9998)
                     /locus_tag="SDD03_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4304671.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DARDHU010000090        10084 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000090 DARDHU010000000
VERSION     DARDHU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.091"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SDD03_002241"
     CDS             162..392
                     /locus_tag="SDD03_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG4304672.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SDD03_002242"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SDD03_002242"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG4304673.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SDD03_002243"
     CDS             1803..1979
                     /locus_tag="SDD03_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG4304674.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="SDD03_002244"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="SDD03_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG4304675.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="SDD03_002245"
     CDS             2474..2941
                     /locus_tag="SDD03_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304676.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="SDD03_002246"
     CDS             3026..3910
                     /locus_tag="SDD03_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4304677.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="SDD03_002247"
     CDS             4024..4260
                     /locus_tag="SDD03_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG4304678.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="SDD03_002248"
     tRNA            4348..4428
                     /locus_tag="SDD03_002248"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="SDD03_002249"
     tRNA            4435..4506
                     /locus_tag="SDD03_002249"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="SDD03_002250"
     CDS             4631..4801
                     /locus_tag="SDD03_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304679.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="SDD03_002251"
     CDS             5021..5728
                     /locus_tag="SDD03_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4304680.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="SDD03_002252"
     CDS             complement(5788..6432)
                     /locus_tag="SDD03_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG4304681.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="SDD03_002253"
     CDS             6559..6846
                     /locus_tag="SDD03_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304682.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="SDD03_002254"
     CDS             6954..7274
                     /locus_tag="SDD03_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4304683.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="SDD03_002255"
     CDS             7355..8668
                     /locus_tag="SDD03_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4304684.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="SDD03_002256"
     CDS             8665..9384
                     /locus_tag="SDD03_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG4304685.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="SDD03_002257"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="SDD03_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG4304686.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DARDHU010000091        10031 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000091 DARDHU010000000
VERSION     DARDHU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.092"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(495..719)
                     /locus_tag="SDD03_002258"
     CDS             complement(495..719)
                     /locus_tag="SDD03_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304687.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            910..3687
                     /locus_tag="SDD03_002259"
     CDS             910..3687
                     /locus_tag="SDD03_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4304688.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3768..4187
                     /locus_tag="SDD03_002260"
     CDS             3768..4187
                     /locus_tag="SDD03_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG4304689.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4199..4669
                     /locus_tag="SDD03_002261"
     CDS             4199..4669
                     /locus_tag="SDD03_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4304690.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4686..5465
                     /locus_tag="SDD03_002262"
     CDS             4686..5465
                     /locus_tag="SDD03_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4304691.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5452..6273
                     /locus_tag="SDD03_002263"
     CDS             5452..6273
                     /locus_tag="SDD03_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4304692.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6290..7339
                     /locus_tag="SDD03_002264"
     CDS             6290..7339
                     /locus_tag="SDD03_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4304693.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7352..8356
                     /locus_tag="SDD03_002265"
     CDS             7352..8356
                     /locus_tag="SDD03_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4304694.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8989..10008
                     /locus_tag="SDD03_002266"
     CDS             8989..10008
                     /locus_tag="SDD03_002266"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG4304695.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1653 c         2094 g         2922 t
ORIGIN      
        1 cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta aaaaagtggg
       61 ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa atatttattc
      121 gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta tggtttggcg
      181 cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc aaaagctact
      241 tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat gtttctacct
      301 tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata aaaaggatgg
      361 tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct cattatgcca
      421 ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga caggaaagtt
      481 gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat caagataaag
      541 atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa acgagtttgt
      601 tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata agatccgatg
      661 atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc cgatttcatt
      721 gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa aactggaagg
      781 aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt ctataataag
      841 atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt tttctatgat
      901 ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga gaaccagaca
      961 gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt agctgatcat
     1021 tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga tgagcaacta
     1081 ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt tgaaaaacaa
     1141 ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga ggaacagcct
     1201 tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc cagtttgaaa
     1261 gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt accacttttg
     1321 attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta tcagtattgt
     1381 ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg tgcacaatat
     1441 gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg agcgtacact
     1501 ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat cttgttttta
     1561 gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta tcttgatcaa
     1621 ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac acgtctcttt
     1681 tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg aagaatacct
     1741 atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct agaactgatt
     1801 tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt cagtggacca
     1861 gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct gaaaaatatc
     1921 gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga aatccatgtc
     1981 acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa tacagcacag
     2041 caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa gcaacagccc
     2101 gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa gaaaaataac
     2161 tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt atttgatttt
     2221 ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg gacacaatct
     2281 atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga tggaaacagt
     2341 gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt accggaagaa
     2401 catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc ttatccatct
     2461 agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga tattgaagac
     2521 acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg gtcaaaagaa
     2581 aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag cagtatggca
     2641 aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat gccactagat
     2701 gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag cgatgtttca
     2761 aatggattga tcatattagt agacatggga tctctgaaag aaatccatca gttttttaaa
     2821 aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt agcaatcaca
     2881 gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga gactgttcga
     2941 cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa agcactcatt
     3001 actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct agaaaaaagc
     3061 ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt aaaagataag
     3121 aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg caccacagat
     3181 cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg ggaaaagatg
     3241 tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga aatattcaat
     3301 cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac tattctagat
     3361 acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt gcagttgaat
     3421 cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg tctcattgaa
     3481 cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg gaagcagtgt
     3541 caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga gcattatagt
     3601 gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga agatacagag
     3661 tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta attttggcat
     3721 gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg gatagagaaa
     3781 ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta gagctgatct
     3841 atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat tttgatttag
     3901 cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt gttctgacgg
     3961 atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg gatcatgtct
     4021 atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg ttggacgctg
     4081 aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa atgatccagt
     4141 tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag gaagaaaaat
     4201 gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt tttcatggac
     4261 acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta cgaatgagat
     4321 acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga tcaaatcaat
     4381 tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt tattcatcgt
     4441 agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa tcaaaaccat
     4501 caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag cggtaaatat
     4561 tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag aagtagaaat
     4621 ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag aaaggaagaa
     4681 cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca caatcagaat
     4741 atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt acaggaatcg
     4801 tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta gcatttatcg
     4861 gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg attttaggga
     4921 cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta ggtttgccga
     4981 ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt ccaattatga
     5041 accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag catatgcact
     5101 tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt tcttatatgg
     5161 tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt caaaaggggt
     5221 tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct cgtttgctga
     5281 tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct tacttagaga
     5341 ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta gtcaatatta
     5401 tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa gatgaagaat
     5461 tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt ttggcgttct
     5521 tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta tgtttatgca
     5581 atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc agctttgaaa
     5641 cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt aggaatcacg
     5701 gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc gatcgatagt
     5761 atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt ttggggaact
     5821 ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa tattttaggc
     5881 cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta tctagcaaca
     5941 ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa cggtatgatg
     6001 gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc aatgactgcc
     6061 acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc tgtgactgtt
     6121 caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt tggcatagtg
     6181 ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact agctttcttt
     6241 ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa tgtttacgat
     6301 gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa agaatgattt
     6361 ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa cgggttcatc
     6421 ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc ttactgtcga
     6481 tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa tcgatactgt
     6541 gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa aaatgataca
     6601 aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac ttgctttaga
     6661 agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg ttacaaaagg
     6721 ctattctgcg actgttttac agttactact cttagggata ggaattggaa taagtaaaaa
     6781 taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa taatcaacca
     6841 tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa gtttatttcg
     6901 tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag ctaaagaagc
     6961 agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt tagaagctta
     7021 tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta ttgaagggga
     7081 aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt acgttggaga
     7141 gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat tagcaataga
     7201 atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat tcgcttataa
     7261 aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact ttgatacagt
     7321 cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag aagaacaaat
     7381 caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca tcgataaact
     7441 ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt atgcttcctc
     7501 gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg tacagcatgc
     7561 tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg ttattttgtc
     7621 ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca agaaagtagg
     7681 agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc tatgcgattt
     7741 tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg cagcagatcg
     7801 attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac agctcgaacg
     7861 ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg gcttatcatt
     7921 tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga atcaatgggg
     7981 agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc catcgaaatc
     8041 tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa caaccccagt
     8101 cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg ctaaactttt
     8161 gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag tagaaggtat
     8221 cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag tgacacaacg
     8281 catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc gctactatcg
     8341 tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag agagcagcga
     8401 taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa acacattttt
     8461 tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg ccaaccattt
     8521 tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag tccaagtaca
     8581 acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa acaaaagttc
     8641 ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga tttagaaatg
     8701 ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt cctaacctcc
     8761 taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac aagcaaagca
     8821 taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat ttcaagtaaa
     8881 aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca actttaagaa
     8941 atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat gaaacaaaaa
     9001 atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt ggcagaaaac
     9061 gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat caataagtat
     9121 catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat cggatataaa
     9181 gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc aacaaaagcg
     9241 attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat cattatacat
     9301 gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt ggctgaagaa
     9361 attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca tgctgaggaa
     9421 gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa agcagcagcc
     9481 tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa tgatgtgatt
     9541 ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg cgctatccat
     9601 ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt ggcagaaatc
     9661 agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt gagtggtgtg
     9721 ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc cggtaacttg
     9781 ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt cgttgagggt
     9841 gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga gatgccaatt
     9901 actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc agcaaaagaa
     9961 atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat agatgatcaa
    10021 tagttaatcg t
//
LOCUS       DARDHU010000092         9752 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000092 DARDHU010000000
VERSION     DARDHU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.093"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..479)
                     /locus_tag="SDD03_002267"
     CDS             complement(<1..479)
                     /locus_tag="SDD03_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304696.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRT"
     gene            complement(579..1088)
                     /locus_tag="SDD03_002268"
     CDS             complement(579..1088)
                     /locus_tag="SDD03_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG4304697.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1289..2158)
                     /locus_tag="SDD03_002269"
     CDS             complement(1289..2158)
                     /locus_tag="SDD03_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4304698.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2178..3299)
                     /locus_tag="SDD03_002270"
     CDS             complement(2178..3299)
                     /locus_tag="SDD03_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG4304699.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3301..3696)
                     /locus_tag="SDD03_002271"
     CDS             complement(3301..3696)
                     /locus_tag="SDD03_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4304700.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3715..4521)
                     /locus_tag="SDD03_002272"
     CDS             complement(3715..4521)
                     /locus_tag="SDD03_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4304701.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4514..5302)
                     /locus_tag="SDD03_002273"
     CDS             complement(4514..5302)
                     /locus_tag="SDD03_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4304702.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5316..5792)
                     /locus_tag="SDD03_002274"
     CDS             complement(5316..5792)
                     /locus_tag="SDD03_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4304703.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5864..8521)
                     /locus_tag="SDD03_002275"
     CDS             complement(5864..8521)
                     /locus_tag="SDD03_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4304704.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(8540..8944)
                     /locus_tag="SDD03_002276"
     CDS             complement(8540..8944)
                     /locus_tag="SDD03_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG4304705.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(9176..9433)
                     /locus_tag="SDD03_002277"
     CDS             complement(9176..9433)
                     /locus_tag="SDD03_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG4304706.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT         3032 a         1992 c         1358 g         3370 t
ORIGIN      
        1 tccgtcctta aaattatatt ttcagtgcca gacaagtgat ttatattgag ttgataaaaa
       61 agatctccat ctaaaaaaat atcattagaa gaagatttcc ccacagtaat gaccattcca
      121 tcagctctat gtgttacata caaataattg tatgacctca tttcacctaa tgaatcaatt
      181 atttcttgcc ctttagctct aatatattga tctctattac ctatctcact cttatttaac
      241 aaaagttttt tcatgtcaaa tttaatcggc tgttttattt ttgtaaattc gcttctacta
      301 ctaatccaat ccgagattaa tttttcaaca tgtcccagag taaactctga tttattcgct
      361 aaatactttt tttgttgtat taaagacatc tttccccgaa gctcaaaatt gagcctacat
      421 cgattctcta tctcttcaaa aagcatctta tcttcatcat tcttatattc atcattcata
      481 aaaccaatcc agctcctcga ttaagataat attttaatct catacaaaaa ctagctctca
      541 tcaaaacaac cattgttttg atttctcatg aaatttcttt atacttttct ccgattataa
      601 attcctaacc ccttgagaaa attacgcata tatttatctc cgcaaggctt aaaatttcgt
      661 tggccttctt ttctcaaaaa tgccccgatt tcgccttttg atggatagat ttctgcctca
      721 gcaaaaacgt ctaaaatatc atcagtggtc atagacaagg cgatttttaa tttttttagc
      781 aacacattgt tgatatattt gatttccttg cttttttgcg gcgtgtctac tatcggctta
      841 tctttttgcg gaccacgcgc caagagaatc aggccattta ggaatgcgtc aaaaacttgg
      901 ttgttgatgg ttttttcata cacattattt tctgcattat ctttgttctt atcctgtact
      961 ttggtcagta ccgcctgagc ttcttcttta gtcagtggta caccgcctag ctcaaaagct
     1021 ttaatcaaat ccgtatcttt caaatcaatc gcataacgca atcgcaataa cctatcattg
     1081 ttattcattt gttcctccga agtatttagt ctttactttc ttacgtactt tttgtcttgc
     1141 taagattttt aggatcaatg ataaaaaccg catcacttat gataaggcgc tgggtatcat
     1201 gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc ggacttcata
     1261 agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct tttgctaaag
     1321 ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg tattcttcga
     1381 tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt actttctttt
     1441 tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt gaaagcatca
     1501 gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag aacggatctt
     1561 ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt ccagatgcca
     1621 acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc
     1681 tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa atcccatctt
     1741 gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg gataaacctt
     1801 ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt gtccaggcaa
     1861 ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc caggcagttt
     1921 ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca atcggtccaa
     1981 atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa tcaattacca
     2041 aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa acctttaaat
     2101 catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag ccatacattt
     2161 cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag tttttcgaaa
     2221 aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc ttgacgctca
     2281 taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa
     2341 ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc atcatcgcca
     2401 aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg ggtgtgatct
     2461 cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc atatgccctt
     2521 tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc tgctcctgta
     2581 gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc tccttccccc
     2641 caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata cccagtgcct
     2701 ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc acggttccat
     2761 ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt aaatgtataa
     2821 aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc tttttttcga
     2881 aatggcatct caaaatagca attcatccct gatgttggat tcactacgat tggtaaggaa
     2941 ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg actttctaca
     3001 gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa gacgaaactc
     3061 ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc agggccttcc
     3121 atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga cccttttctc
     3181 ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt tttattttct
     3241 ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa acaattcatt
     3301 ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga tttgttgttg
     3361 tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca aagcgattga
     3421 cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa
     3481 aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt cgtctgtagt
     3541 atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat atgcacataa
     3601 gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa aaccctcagc
     3661 tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt tcctttacgc
     3721 taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa tcaaaatggt
     3781 catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa ttactggaat
     3841 taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt caccaaaagt
     3901 gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa ctactgttag
     3961 ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt cagtaacaat
     4021 cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga agattaccat
     4081 gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca tataagccgc
     4141 aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa taccagcaat
     4201 tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta atccttgctt
     4261 ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct gtgtattgta
     4321 atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt ataattttct
     4381 taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt
     4441 acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg ttacttgata
     4501 tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct cccgcagaag
     4561 cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa gcgaatccta
     4621 tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat ccaacaagta
     4681 aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata ccaacagcag
     4741 gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa acatccaaaa
     4801 cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt gctgcagata
     4861 aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat tctcgtttat
     4921 tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca attacatcta
     4981 gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct actccaagct
     5041 ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca gatgagccac
     5101 caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc aaacctgttt
     5161 ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa tttacaaaga
     5221 tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc agcataataa
     5281 tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga ttgaatttcc
     5341 tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc caacaatttt
     5401 ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg taatgcatta
     5461 tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt atctactaaa
     5521 tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc gtatttcccc
     5581 tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat tccagcaggt
     5641 ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc atcgatcacc
     5701 atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc atgcaataaa
     5761 cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat ctttcatttt
     5821 cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct ctttttacat
     5881 aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa tgttcaacga
     5941 cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc gtttcattga
     6001 aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag caactaatgt
     6061 gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca ttttgtaaga
     6121 tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaatatg gtcaattgct
     6181 ccattaaatt cgttagacta aaattcttca gaatattatt gttcaaaact ttcagttcat
     6241 tatcattaaa gtgtctattc aattgattgg agaatttact tatattcatt cctaaaatca
     6301 tatcttcaat tgaaaagaat tctacatctt ctagatgtgg atttagtgtt ccaacaataa
     6361 aaagaatatt gttctcttta cgaagctccg ataaatagtt ttcattcact aaagagtaat
     6421 agtcacacgt ttctatgaca agatcagtat cccttggaaa gctatcttca agtatctttt
     6481 ttaatttaaa cgaagttcct aatccgcttg cacaagaaca gacaatcata tccttcttaa
     6541 tatttgatga ttcataaata taatccacat tttgattttc ttttacttgt tcaagaatct
     6601 gtttaagcgg taaatctaat aatatctgat ttgcaacatc aatagccatt cttgttgaaa
     6661 cattgtttag tagtccgaat tttttttgag cgtctagttc caaatttaga taaattttct
     6721 ccagtgaacc catatcaaca agtaaaataa tttcttcata tccggtacac tcttgtaagt
     6781 atctattaat tttgtctgca atctcactac ttgaagtcat tagtggcata tcaatggcgt
     6841 cgtatatata tctctcaacg atcctattag cagtacttgc tatacaggtt gccgttagat
     6901 ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa ttctttacta cacattaact
     6961 taaataaaat aaccaaaaca tttttttgaa tttttgttaa ataaatatcc cctatttgaa
     7021 taaaacgatt gactaattga acttctcgac tacaccaact attaatatca ttgatgaagg
     7081 aatataattt atcgtccgat gttaattgga tttgataaat ccaagaattc aaaaagatag
     7141 acgttatttg aagataataa tagtcaaatt ctatttgaaa atctctctct atttccttaa
     7201 catactgtct aaatcgaatc tcttgatagg tgggatatgt taaagctgaa ttattttgga
     7261 tatcaacatt cttttgtcta aaatatctat catatttctc aaaaagctgc tgcttatcag
     7321 tgtctttctt ccacgtttga taaagatact ctataagttc gttgatatta aaaacttcgt
     7381 ctactttttc caaatcatcc atttgaatat attttaattc attctgtctt ttcataacct
     7441 tcatgccgtt gaaattcacc cattgtggta aatgatacat ctgaatataa agttcttcca
     7501 aatcatctaa gaaataaaag gcattggcac aggtcaattg aatgatattt ttcaaattgc
     7561 caacattttc actaaagcga tagtttaata aaaaatctaa taattgttga gatatcagta
     7621 ttttctttgc tagtctttct tcttctgatt taaaaaatat cttgattaac tgttttcttt
     7681 cataaagaga acgactgtct aatgttggaa gtgttagctt tacaggtatt cttctcaaca
     7741 aagttgataa tagtttatcc tcaggtggtt ctgatgttgc aaatataatc cagcaacttg
     7801 atgtgtacca gttttcatta tcacccatcc gatgaaattt cctcgtatcc ataaactgaa
     7861 aaagtttctc ctgacattca ttgctcaatg catgaatttc atctaaaaat aagactccat
     7921 tattagctaa ttctgctaaa ccctttcttc catcttcggc tcctgtatat gcacctttta
     7981 catgcccaaa aaaattggac aagaaaagtt ctggattatt ggcatattca ctacaattaa
     8041 ctgaaatgaa agatgccttc tcttctatat aattattggc caacaaatcc ttatacacat
     8101 aatttgctaa atagcttttt ccagttccag tctcgccatg aagtaaaatg ggtaagccat
     8161 ttggcggata gtagattgct gctttaattt gagcaattac atttcgcaga cttccttgac
     8221 ttccgataac cctttctatt ccaaccctaa aagagctatc ataccatttt tccgcttcct
     8281 ctgaagagct aaaaatttgt tgatttggaa ctttctccgg taaagaaaag aagattactg
     8341 gacgagtatt gatttttcca aattctccat cttttacgaa ttcattcaaa tattgagaga
     8401 caatattacg actaacattt aatttttcag atattttctg tgctgtattg ttcccccatt
     8461 tatttttaat ctcagagttc tctatgacat aattgacaat gcgttctttt aattcttcca
     8521 tcacagcacc tcctcgacac tattcgaatt ttgccgacag tatttttcca taaatatgac
     8581 ttgctaaaaa ggctttgttt agatcattca attggaacgt tgcttgtttt gcttgatccg
     8641 ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc caaaatatca acgattgttg
     8701 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac atcgtttgtt
     8761 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     8821 aacggtcgga taaatattta tagaaaatca gccccaataa ataattttta tattctgacg
     8881 catccatttt actacggaga ttatctgccg cactaaataa cttctgattt aattctgcac
     8941 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     9001 actcagtttt agaaaaagca aaaaaataaa gaaacttatt cttgataaca agctacgtga
     9061 cgtatatccc taacacaaaa atagccatat cctttggttt ggatacggct atttgatatt
     9121 actcattcaa ttatcaaatc acagtaggtg tgaatctcat cgggatcggc aatatctact
     9181 tctgattagg atcaaaggtg attttctcaa taaaggtgtg atacaaagct ttaacttgct
     9241 tcttctcact ctggataaga aagcaatcca aactagtcaa tatctgttgg acatctttga
     9301 cttgaatcgg ttccccttgt tgttgaaaga tccctaagat ttcattctct cgaatttgta
     9361 actgtcttcg tttttcagtc aattcattta aacgatcttt taacatcgga aacaattcgg
     9421 gactatcttc caaagcagct tcctgttttt gctctaatag ttagattttg cgtaccattt
     9481 tcttctttta ttttccttcg attgctgatt ttaatatagt ctaaaggaaa tttatattta
     9541 ttgtattctt tataacttca agagaacttc gtttgagtat tactgtattt ttttaattcc
     9601 ataagaaaat tagttattac ctgccaccta atcagcaaat ctgaacctga gtcaatataa
     9661 ttaatattat tttccatctc caacattgta agtactgttg gtgttaatat agggactttg
     9721 tacaattgta tttttttgat tttatcatct gc
//
LOCUS       DARDHU010000093         9659 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000093 DARDHU010000000
VERSION     DARDHU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.094"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            179..2107
                     /locus_tag="SDD03_002278"
     CDS             179..2107
                     /locus_tag="SDD03_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG4304707.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="SDD03_002279"
     CDS             2245..2700
                     /locus_tag="SDD03_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG4304708.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3170..3439
                     /locus_tag="SDD03_002280"
     CDS             3170..3439
                     /locus_tag="SDD03_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304709.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /locus_tag="SDD03_002281"
     CDS             3839..4093
                     /locus_tag="SDD03_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG4304710.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4182..5228)
                     /gene="holA"
                     /locus_tag="SDD03_002282"
     CDS             complement(4182..5228)
                     /gene="holA"
                     /locus_tag="SDD03_002282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG4304711.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5266..7539)
                     /locus_tag="SDD03_002283"
     CDS             complement(5266..7539)
                     /locus_tag="SDD03_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG4304712.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7532..8071)
                     /locus_tag="SDD03_002284"
     CDS             complement(7532..8071)
                     /locus_tag="SDD03_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG4304713.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8207..8425)
                     /locus_tag="SDD03_002285"
     CDS             complement(8207..8425)
                     /locus_tag="SDD03_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG4304714.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8422..8667)
                     /locus_tag="SDD03_002286"
     CDS             complement(8422..8667)
                     /locus_tag="SDD03_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4304715.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8783..9511)
                     /locus_tag="SDD03_002287"
     CDS             complement(8783..9511)
                     /locus_tag="SDD03_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG4304716.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2987 a         1875 c         1678 g         3119 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa
     4141 aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac
     4201 tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg
     4261 atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat
     4321 ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca
     4381 atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat
     4441 tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg
     4501 tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata
     4561 ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga
     4621 ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg
     4681 gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga attctctaag
     4741 gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt
     4801 gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca
     4861 aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta
     4921 ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat
     4981 actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa
     5041 cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt
     5101 tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag
     5161 taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta
     5221 ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt
     5281 ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg
     5341 atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg
     5401 gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt caatatggga
     5461 aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc
     5521 tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa
     5581 aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc
     5641 ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc
     5701 atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa
     5761 tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga
     5821 taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat
     5881 tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat
     5941 cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat
     6001 cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa
     6061 agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc
     6121 aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc
     6181 gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga
     6241 agaaaaatcg attattcgct caaataagat taatccctct tctatcagca aaagtaaagt
     6301 ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt
     6361 gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg
     6421 ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa
     6481 ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc
     6541 acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa
     6601 taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag
     6661 caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag
     6721 tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata
     6781 cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc
     6841 actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa
     6901 agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga
     6961 tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt
     7021 ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt
     7081 ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc
     7141 ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag
     7201 ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc
     7261 acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga
     7321 atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc
     7381 gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata
     7441 accccaattg tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat
     7501 caaccgattt cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt
     7561 gaagttttga aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt
     7621 cgatcaacgc ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca
     7681 caccagcttg caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt
     7741 ctgaaccttc cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat
     7801 ggatcgttcg caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat
     7861 ctccactgac tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg
     7921 ctcctacttc taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa
     7981 agtactggtc ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg
     8041 tatcgcatgc ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg
     8101 attaaaaagt actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct
     8161 gacaaaagat cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa
     8221 aaattcttcg aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat
     8281 ggcgttttgc tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat
     8341 atttttgttt tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat
     8401 agtctttttt cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag
     8461 ttgtttatgc tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata
     8521 gctaagtaca tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc
     8581 cgtattcata atatgaatcc cagatatttt attttggaga tagacattca gcattggttt
     8641 ttttaatgtt tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt
     8701 cctgccaagc tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg
     8761 tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg
     8821 tcttttcacc gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt
     8881 cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa
     8941 gttctgatgg agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct
     9001 gttttccttc cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag
     9061 ggatatatag gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg
     9121 cttcttctaa taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa
     9181 aggaatagat accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac
     9241 gagcatcctc ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg
     9301 aagaaagtat ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata
     9361 ataaaaaacc acttgcgata caaaagataa gaactatcat agacatccaa atgatcaatt
     9421 tgggtgaagc tagtattttc ttgctgattt tttctatcat tgttcttttt tcaaagaatg
     9481 accagtaaaa atcattcatt actcctttca tttaaaattg aagaaacagt gagctctggc
     9541 aaaatcgtcc cttcaacatc taattacgtc aattttttct tttgttttta tttttatcgt
     9601 gaagattttt gacgaaaaag aggggttgtg acagaactcg tgtcacaacc cctctttaa
//
LOCUS       DARDHU010000094         9443 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000094 DARDHU010000000
VERSION     DARDHU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.095"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..479)
                     /locus_tag="SDD03_002288"
     CDS             complement(57..479)
                     /locus_tag="SDD03_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4304717.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(469..2643)
                     /locus_tag="SDD03_002289"
     CDS             complement(469..2643)
                     /locus_tag="SDD03_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG4304718.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2659..5001)
                     /locus_tag="SDD03_002290"
     CDS             complement(2659..5001)
                     /locus_tag="SDD03_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304719.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5023..7566)
                     /locus_tag="SDD03_002291"
     CDS             complement(5023..7566)
                     /locus_tag="SDD03_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4304720.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7576..7974)
                     /locus_tag="SDD03_002292"
     CDS             complement(7576..7974)
                     /locus_tag="SDD03_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4304721.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8055..8339)
                     /locus_tag="SDD03_002293"
     CDS             complement(8055..8339)
                     /locus_tag="SDD03_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304722.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8363..9361)
                     /locus_tag="SDD03_002294"
     CDS             complement(8363..9361)
                     /locus_tag="SDD03_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4304723.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2690 a         2015 c         1471 g         3267 t
ORIGIN      
        1 aatttgaact cataacaatt ttttgttttt ttcaacgtat tctctttcaa ttttcactaa
       61 aaattaggaa gaaattgatt caatgtcttt tcaaaaaaga atgtttttct ctcaattttt
      121 ttgacgtttc tatcatgaat cacgagaaaa cttgggacac ttttaacatg gtaacgttgt
      181 aaaaaagttt gaagttcttc ttgtcttggt tcttttcttg tttcaattcg atatacattc
      241 ctagagaatt ttttgtcgta tctttccaag aaagattcaa cttcttggca ttttttacaa
      301 tcatttcttg ctagatacac aaaaaatgat tgattcgttt tttgaagttc tagtacttct
      361 tctaaattac tattgattag ttttccattt gattcttcgt tagtttttat cagtttaaaa
      421 caaccaaata agaggaaact aacaataagt atcctaacaa taactttctt atttcgcata
      481 ttgtttctcc aaatttggtt tccgaatata gacgactcgc ggtggttcct ggacgtcttc
      541 agctgttcga tacaccacaa attcgcctgt tctcaatgct cgaatgcgat tcggattgaa
      601 tttaaagcgg tctacgtctc gtttcgtgcc tctttctcct gtccaatcaa cctttcccat
      661 atcagaataa cctgcttctt gtgtcgtcat ttccgttaat tcaatgtctt cttctgtccc
      721 cattaaatct gcccaataat caatttctgt cgggtcattt gtcttcccaa taaagaacgt
      781 attcacattc ccaatcaact gttttcgaag gacaggatca attctatcaa tgtccgcaag
      841 cgtttgaggg gcatacgccg catgcacccc agcgcctcgt cccttattga ctaagtctat
      901 aaggttatca tttagataag aagctggttc gtcaaatatt actaggactt tcgagcgatt
      961 ggctgtttct cctgcttgtt tatacacttc tgacgcaaag aaattaatat ctcccacaag
     1021 catttgagcc aatgtccgaa taaactcact ataaatcaat ccgtttaacg agatataaac
     1081 aatctttcct tctttaacaa cttcttgaat atctaattct ctttttcctc cagaagtatc
     1141 aaagagttca ccaagctcac tatccaataa catattgata ttggtctgta aattctcaga
     1201 agtcgcatac aaataaaacg ggttcttctt atgttcgtaa cgaacaaaca accgtttaaa
     1261 caacgtttgc tcgatttctg tcatgagaga aatgttttct tttaataata gataatacgt
     1321 ttctaagtca acggttttac gatcatgttc aacgattcgc tcttcactta actgttccac
     1381 atcggcaaaa tcacgttctg ttttgtccat cgcttcattc tttttcgttt ttttcttcgt
     1441 ggttttcttc ttttctacga ccactttttc tttaaataaa tctggatttt tcaactgaat
     1501 acgatccgca aacaaatgta agacgtttcg tggcaataaa taatgttgta accaaactag
     1561 atttctttca attttatggc tcttttggaa ctcgtctaaa agctgaatcg ttacctgtaa
     1621 taacgctttg gctgctgtcg aataatacac gctttctgtt tctgctagat tggttaattt
     1681 atctcgaatg gcaatatcat tgccattttt tacaggatta taacgcaaat cgccaccgtc
     1741 cacaaagact tcaatcgggc gattgtatgc ctctgccact tcttttaacg cttgtaaagt
     1801 ctctcctgct cctttcccat cgactaagat aacggggata cgaaacttac acgcatgttc
     1861 tgctagtaat tggatcaccg tcgtttttcc tgaacctgta gtcgctggaa taatcacatg
     1921 ttggttcaat tcactaaagt tcatcacata agggagccca tactcatctg ttcctaatag
     1981 aatcactttg gctaatgaag ccagttgttt attccgcaaa gcaacgtctt ttatgacaat
     2041 tgctacctgg tacgcttgct gatactctgc tgtatatttt tgggactcct gaaatttatt
     2101 cttccgacgt tccttgtatt ctttactatt tagcaaagct gtttgccgtt ctttctttga
     2161 aacaactttt cttttttcaa taaaagacgc atacgcaatc aaacaacttc ctgcacctgt
     2221 cccacctaaa aggagggcaa aaatcgtttg tccattaaac tgcaacgcat gagaaaacaa
     2281 gtttaatgga aataataagt aacacgacaa ataccctcta ggaagaacaa aaataaaaca
     2341 aaatagattt aatagcgtaa aacctaacgc aaaaataaaa aaaagagagc ttcttcgttt
     2401 taagaagctc tcatactcaa agtcataaat atgtgcacgt gtctcttttt tatctatcat
     2461 aaagacaatc gctaaaggaa tactgcctac aataaaaaat ggatatagac aaacacctaa
     2521 taaagtgacg ataaaataaa gttgttgaaa ccgacttttt ggcgtttctt tttcttgttt
     2581 tcttttgcga tcgacagaaa acatttgctc aaaatgtgtc ggtcgttttg gtgcattcat
     2641 catactatcg atttcctcct atcgttttcg ttgtttcatt tctcttcgat aaatcggttg
     2701 tttttctcgt tttcgtttca tttttgcttg agttgctcga aactcactcc gtgtcttcgg
     2761 tgcttgtttt ggtgattttg tttgtttcat ggttgagtat gctttagata cggttgaagg
     2821 cgctttggtt tgtgctgtta ctcgattgac tgctgacata tgttcccgaa ccccctctgg
     2881 acgtcgaaca gatttcggtg tggtagaacc tgttgctcgt ggtacattcc cttttcttag
     2941 tggtttttgt accgcatgtt tttgtcctgc aactgtccgg gttttcatcg aacttgtttt
     3001 tgttacggga gtaagtgcct ccttccgacg acgacttgtt tgtccctgac gacgttttcg
     3061 atcgttcaat tctgttctag gcgtatgctt ctggccataa gacttgtgtt tttgctttgg
     3121 attcgctaat gacaccacca ttgcagaacc ctgattgcga tacggactac gagacgcacc
     3181 ttttctactt ctttctctta gactaggatt gccttgcaat tctcgttgta ataaatcggc
     3241 tgtttttctt gcctgtttct ccattgctgt tcgttgcata cgttcttgac tttgctcttc
     3301 tgcttgtttc gcttgatcat gcaagtctct tgctttttgt gccatacgtt ccatacgatt
     3361 tggtgcttta tgacgaggtt gttgtgcgga ttcacgccga aaagcacttc gtgtggcttg
     3421 tttcgccgtt tgtggcatac gaacgtctga gccaccccca cgttttcgtt gacgttcttg
     3481 tctcttccgt gcgttttctt tttctttttg tttctgtgct tttctcgctt ctttgcgttc
     3541 tttcaaccgt tgtttggctt cccttctcaa ttttgcttct gccccaaatg gatttcccgc
     3601 aaaagctaac gctcctcgaa cacccattct tccagtaaat agactgccta aaaagcctcg
     3661 gaacaaataa attcccaacg gcgcaaacaa ataaacaatg atcaagagaa ttttttccca
     3721 tggatcgtct tgtgtactac ttaattggaa cccgtaacta agaaaagaag aaaaaaggat
     3781 acatgcaaaa cctgtcattc cttttaaaaa cgtcaaaata ccaaaaactt tggcatagtt
     3841 accaaacaca ttcattgtga caagaaacaa tcctaaaaaa agtaagaacg gcaacatcgg
     3901 tactaacaat agttgcatag ttccaataat caatcgaaac atacttaaga caaaataaac
     3961 aacggtttga atcagattca cgacaaaata aaacaaggca aatgctgtat ttttccaact
     4021 attgtaatat tgtatgttct tgtttttcag tgtatctgct tcatactctg tgactttgtc
     4081 atgaattttc tcccctattt ttgtacctaa atcattgtcc aataaaagat tgattcgatc
     4141 atattctttt ccagcatatt tgactttctt ctcacgaatt gtgtctacat tggttgtgcc
     4201 ataatttaac aacagatatg gttcataaac attactataa aagacccgag ccgccatcaa
     4261 tttgcctgcc gtttccactt ttttatccgc tgttacctct tctgtttctt ttgtttcata
     4321 atttgtttct tcttcaggtg ttaacactgg attgaccttc acgaacgccg tttccactgt
     4381 agaatctaaa gaaaaaagaa catcaaaaag attcttattt gtcccagaat cttttaatac
     4441 gcttaaccca gtaaaaataa ggatcgtcat aagaaagaca cggagtgctt gtttcattcg
     4501 agcctctcca gcatatttga ttgccatcat aattgccgaa aaactgattc caatagttcc
     4561 tgcaatactt aacatattgt ccgcaatttt ttgtgttaag ttttgaatgg gttctcgaat
     4621 ggcggaaata atatctaatt caaagagaaa ttcaaccatt gaagcattga attctcctaa
     4681 catttttacg ccactccaaa caaaattttt catcactcct gcaacattgt acgatagctt
     4741 ttttttaagg ttcattgact catagtcatt ggtcatcaat tcaaacgaat tctttttgta
     4801 atttttatac aattgatcca catcagtcgt tacttctttt acttctactt tatcgttggc
     4861 attctctcct tcagaacgat cggtttcagt gacacccgca ccatcattca tttgatcggc
     4921 agaaacagta gccccttgta gacagaaaaa aagaccccct aataaaagga gcgtccacca
     4981 tttcagagat ttttttcgca aaaaaaccac ctcttttttg ttttatcgtt gtttttcatt
     5041 ttctaacatt gcttgaattt tttcatcttg attttcagta gaagaaacag ccaataacca
     5101 actatcaaac aacacatcaa tggcaatcgg ttgatttctt cccaaatgat cctggaataa
     5161 acaggttcct gtttgtaatt gttccatcat ttctaaattg gctgaattca cttccatatc
     5221 aaagaaggac aatagtttct ctcgtgcttc tttttgtttc ggacgaaacg caaatttgta
     5281 agaaattaac tcttttttat cttcttgatc gtaatcttta aacgcttgca caacaaggta
     5341 aatatccgta ttattcgcac gtccttttcg caagctatca tcaatcaaat agcttccttg
     5401 tgcggtatct tcataccctt ttgcctcgtc ataaataatc gcagcgtctt cttctttgtt
     5461 tgtagaaaag atatacgtaa acttcataat gacttccata attgccattc ctgcaagttg
     5521 ttcgctattt aatcgacccg aatcaatctc tttttgtgtc ggaatgcgta ttccttgtgt
     5581 ccctaacacg ttgatttgtg aatcaaaacg tatcggcgta ctgtgctcat tcccaatgag
     5641 aatctttcct aaaccaacgc tatgccctaa aatcaattta gctagttctt tgtcactttt
     5701 tcgaatttct tcgataactt gacttaacga atgcttacct ggcatggtgt aaactacatc
     5761 gatggcgtct tggatcaccg ttttcttgtg gctcgctgtt tctcgattgg tatctacttc
     5821 tcctaacatt cctaacacat ttttagcggt ttgaattgcc tcttcttttg gtaaaaacaa
     5881 tagaggatca agcatccctc gacagctttc atctgaagat aacgaaataa aattaattcg
     5941 ttcatacata gccacaaact ctggtatgtg tccgaatttt tcaagtgcct ttaaaaagaa
     6001 cttttgggtt tcatttttcg gatcaatata aagaattttt tgacctaaaa acatcagcca
     6061 taaaaaaata tattttacaa ggaccgattt tcctttacct ggtggaccaa taatcaacgt
     6121 atttccattg gtatgttccg cgccttcaat acttttcttt gttaaattgg gataatacca
     6181 tactagttta ctagatagcg ctaacgcttc tttcgcagat ttaaactttt ttgcaataac
     6241 aatccgtcct aaaggaaatc ctttcttatt tcctacttct ttctttaagt ccaagccata
     6301 atctgccaca aaacctgtcg tagcgacctg ctcgtaacta cgaaaacgtt gcgtacttcc
     6361 aattaagcat tgattaaaca acgttagctg atccgctacc gatcgataaa atttgaaatc
     6421 tgtagactca atcgcaaatt caatatcttt gactcgttct tccaattctt cttttgaaga
     6481 agcagataca accagcgtga cggtagaata acacaatcgt ttttctttgc gcttcagctt
     6541 atcttttaca tccattaacc gttctgtacc aaaatttaat acatcgtctg aatctaaaat
     6601 gccgtcaacg gtctcatatt ctttttcttg ttcataaatc cgtccagcca ttttattaat
     6661 ttttcgttca tcagattttg aatgctcaaa tcgaagtcga atatgcgtat caattggaaa
     6721 agataagcta tcctgaagat tttgaataaa gctactccca tacatggcta aaggagcgtc
     6781 cgtttgactt aaaaacgtcg aataatgcgt ttgattcaac tgttcaatcg caatgtatcc
     6841 tgcttcattc gaaatgacgc cctcgcaaat ttccattggc gtaagctgtc tttcaggcaa
     6901 tttttcttct gatcgatgga aaaaataata ataaatttgt ccaatctctt gggcagataa
     6961 gcgctctatc ggcttataat tggacaattc cgtatagagc tccttttcta atgtttcata
     7021 cgttggtaac acactactta taggaactcg ttgtcccgta atacgataga taaacttttt
     7081 aaaaacatgc ttaagcaatt gaaaatattc gatcggatca gacactttca tttctgtagt
     7141 gaaacgcaca aacagatacg ttcgatactc gtttaattgc acttcgtctt ttaaaatctt
     7201 tcctgcctgt tcaaagtaca ctttcccaag atctgcgcat tctcccttta ccaaatctcg
     7261 aatggtttct tcaatgtgcg tatctaggtt aaattgacgt ggaatattga taaaatgata
     7321 gtcatattca tcatgtccaa gtatgccttc gccatcttga agatagttct tgaaaaaatc
     7381 aatgtcattt aaaggaaata cttgggaaga aagttgatag cctacccaca catcatcatt
     7441 tttggtcaaa acaaaatttc ggataatggc gtctatcgga aattctaatc gaggaagctt
     7501 cgtttttttc tttttgaact gccaaatccc gataataaag acgaccaaaa gaaacaaaaa
     7561 cgccatttac tcgactcact ccttccattt gtgtagggaa tacgtcactt tttcatggaa
     7621 aaacggcaca tacatttcat gttcaaagcg ttgtttccgt ttctgataat aggacgttcg
     7681 cccaattaaa tactcgaaaa agtgtttatt gtcccatttc attgaaaaca atgtccaaac
     7741 gagaacacca atcccaaaaa tcataatcaa ccaggacttt cgaaacagtg tctgcaagaa
     7801 actaatttga ttcacatacg caatcaaaaa taggatcaaa agaatgaaga gaaatacccc
     7861 acctatgact aattgttcca tgcgaatgcc attagaaatc cagggaattt tttgtccttt
     7921 aatcccgtaa attttgtgag ggtgcttaaa ctcctttgta tagttgtatt ccatgttcac
     7981 tctacctcct tttacaaaaa aagaagcgac cagaagttct aatcttctga tcgcttttgc
     8041 agtttgtctt tttcttatga taattgtatc cagggcacaa tcatttgcgt tacccccacg
     8101 ttctcttcac tatccttaat aaagataatg ttctttagcc attcaccaat atcaacggca
     8161 ttcgataaca ataagacgag cattatcaaa ataaagacgc caataatggc aaacgctgcc
     8221 cgcgcaaagc cttgggtcgc ataagcacca accattagaa caatcgcaac gattgccaat
     8281 agccaaatga cttgtccaga tacatcatca aataacgatt taagatcaaa aagtttcatt
     8341 tccattctcc tctaagttca ttttattcat ttaacgtagt aacaaaataa ctatcgccac
     8401 ttgtttttac ttccattgaa aagtttgatt ggaactcgtt tcctttctca aaatagaaac
     8461 gatacttccc ttgcaaaaag tagtttcctt ctttattttt tactgcatga tccagttgta
     8521 cattgatcaa ttcgccgcct tctaaccctt tttcgttaga aatcaatgcc aatttttcat
     8581 catttttgac atataactca aaaaaccgtt tcacaaatgc ttccagcttg atttcttcgc
     8641 tttcagtgac cgcttgccca gaagaataaa atcgattttc ctgatagttc acagaattgg
     8701 tattcttact ctcagaaaga ttcaaaaatt gaacgccatc aagtactttt aaaacattgt
     8761 tttgataact gactggaaaa gaaagttgac tgcttttcca atgttctcct tctgtaaaag
     8821 taacctcata aaataggcga tacattgctt gtgtcaaatc gatttcttct agtttaataa
     8881 agcgaacatc tttcgctact cgattggtag ccgtcgttac aaagcctaga tcagacgctt
     8941 ccaatccttg tgctaaataa tgattgacgg aagcctccca ttcgtttcgt ttttctggcg
     9001 aataagtaaa taacctttca ataaaatgtt gtccctcgta cttcaattgt tccgtagaag
     9061 acaaggtttg gttgacttca gttaacaatg tttctggatt gacttgtttt ttcactgcta
     9121 aacgcgttaa tgtatccact cgtccaaagt ttaggaggag cacgacaccc attaacagaa
     9181 acaaacaaaa aaaagcgcct gaaaatacac tcatggcact tttttgcgaa aattggatca
     9241 ttcgatctga cggttctgtt ttcttttttt ggaacaaaaa acgtttcttt tgtttctttt
     9301 ccttctgttg tttcggtggc ttagtttgga acgcttgtgg attaaatttt gcattactca
     9361 tgagaaatgc cataccgttc taacaataat ttcggtattt tgtccacaat cactacttcg
     9421 tggacaacca cacttgcccc ctc
//
LOCUS       DARDHU010000095         9407 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000095 DARDHU010000000
VERSION     DARDHU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.096"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SDD03_002295"
     CDS             99..293
                     /locus_tag="SDD03_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304724.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="SDD03_002296"
     CDS             562..861
                     /locus_tag="SDD03_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304725.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="SDD03_002297"
     CDS             complement(958..1392)
                     /locus_tag="SDD03_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304726.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="SDD03_002298"
     CDS             complement(1624..2334)
                     /locus_tag="SDD03_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG4304727.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="SDD03_002299"
     CDS             complement(2324..3184)
                     /locus_tag="SDD03_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG4304728.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="SDD03_002300"
     CDS             complement(3189..3827)
                     /locus_tag="SDD03_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG4304729.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="SDD03_002301"
     CDS             complement(3842..4141)
                     /locus_tag="SDD03_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4304730.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="SDD03_002302"
     CDS             complement(4184..5668)
                     /locus_tag="SDD03_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG4304731.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="SDD03_002303"
     CDS             complement(5688..6149)
                     /locus_tag="SDD03_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4304732.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SDD03_002304"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SDD03_002304"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG4304733.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="SDD03_002305"
     CDS             7452..8222
                     /locus_tag="SDD03_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4304734.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="SDD03_002306"
     CDS             complement(8495..9322)
                     /locus_tag="SDD03_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4304735.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2923 a         1931 c         1478 g         3075 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaaca
//
LOCUS       DARDHU010000096         9369 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000096 DARDHU010000000
VERSION     DARDHU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.097"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..656
                     /locus_tag="SDD03_002307"
     CDS             33..656
                     /locus_tag="SDD03_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304736.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="SDD03_002308"
     CDS             1106..2155
                     /locus_tag="SDD03_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4304737.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="SDD03_002309"
     CDS             2152..3033
                     /locus_tag="SDD03_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4304738.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="SDD03_002310"
     CDS             3036..3806
                     /locus_tag="SDD03_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4304739.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="SDD03_002311"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="SDD03_002311"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG4304740.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="SDD03_002312"
     CDS             5117..6280
                     /locus_tag="SDD03_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4304741.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="SDD03_002313"
     CDS             6397..6807
                     /locus_tag="SDD03_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4304742.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="SDD03_002314"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="SDD03_002314"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG4304743.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         3262 a         1588 c         2022 g         2497 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttt
//
LOCUS       DARDHU010000097         9280 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000097 DARDHU010000000
VERSION     DARDHU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.098"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..435
                     /locus_tag="SDD03_002315"
     CDS             <1..435
                     /locus_tag="SDD03_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG4304744.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="SDD03_002316"
     tRNA            complement(759..842)
                     /locus_tag="SDD03_002316"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="SDD03_002317"
     CDS             1370..1870
                     /locus_tag="SDD03_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304745.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="SDD03_002318"
     CDS             complement(2060..2404)
                     /locus_tag="SDD03_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG4304746.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="SDD03_002319"
     CDS             complement(2558..3478)
                     /locus_tag="SDD03_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4304747.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="SDD03_002320"
     CDS             3665..4156
                     /locus_tag="SDD03_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG4304748.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="SDD03_002321"
     CDS             complement(4180..6555)
                     /locus_tag="SDD03_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG4304749.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="SDD03_002322"
     CDS             6881..7507
                     /locus_tag="SDD03_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4304750.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="SDD03_002323"
     CDS             7530..8234
                     /locus_tag="SDD03_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4304751.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="SDD03_002324"
     CDS             8426..9259
                     /locus_tag="SDD03_002324"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG4304752.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3010 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct
//
LOCUS       DARDHU010000098         8933 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000098 DARDHU010000000
VERSION     DARDHU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.099"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(93..761)
                     /locus_tag="SDD03_002325"
     CDS             complement(93..761)
                     /locus_tag="SDD03_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4304753.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="SDD03_002326"
     CDS             895..1182
                     /locus_tag="SDD03_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304754.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="SDD03_002327"
     CDS             1598..3136
                     /locus_tag="SDD03_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG4304755.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="SDD03_002328"
     CDS             3133..4098
                     /locus_tag="SDD03_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4304756.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="SDD03_002329"
     CDS             4085..5407
                     /locus_tag="SDD03_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4304757.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="SDD03_002330"
     CDS             5648..6391
                     /locus_tag="SDD03_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4304758.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="SDD03_002331"
     CDS             6406..7398
                     /locus_tag="SDD03_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4304759.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="SDD03_002332"
     CDS             7410..8147
                     /locus_tag="SDD03_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4304760.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="SDD03_002333"
     CDS             8137..8859
                     /locus_tag="SDD03_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG4304761.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2980 a         1518 c         1868 g         2567 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aat
//
LOCUS       DARDHU010000099         8891 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000099 DARDHU010000000
VERSION     DARDHU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.100"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1014
                     /locus_tag="SDD03_002334"
     CDS             103..1014
                     /locus_tag="SDD03_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG4304762.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1501..1767
                     /locus_tag="SDD03_002335"
     CDS             1501..1767
                     /locus_tag="SDD03_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304763.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            1839..2180
                     /locus_tag="SDD03_002336"
     CDS             1839..2180
                     /locus_tag="SDD03_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304764.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2235..2654
                     /gene="ssb"
                     /locus_tag="SDD03_002337"
     CDS             2235..2654
                     /gene="ssb"
                     /locus_tag="SDD03_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4304765.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            2731..3027
                     /locus_tag="SDD03_002338"
     CDS             2731..3027
                     /locus_tag="SDD03_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304766.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3042..3473
                     /locus_tag="SDD03_002339"
     CDS             3042..3473
                     /locus_tag="SDD03_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG4304767.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3655..3960
                     /locus_tag="SDD03_002340"
     CDS             3655..3960
                     /locus_tag="SDD03_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304768.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4364..4852
                     /locus_tag="SDD03_002341"
     CDS             4364..4852
                     /locus_tag="SDD03_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4304769.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            6186..6488
                     /locus_tag="SDD03_002342"
     CDS             6186..6488
                     /locus_tag="SDD03_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4304770.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            6481..7509
                     /locus_tag="SDD03_002343"
     CDS             6481..7509
                     /locus_tag="SDD03_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG4304771.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            7565..7783
                     /locus_tag="SDD03_002344"
     CDS             7565..7783
                     /locus_tag="SDD03_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304772.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            7854..8669
                     /locus_tag="SDD03_002345"
     CDS             7854..8669
                     /locus_tag="SDD03_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304773.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            8733..>8891
                     /locus_tag="SDD03_002346"
     CDS             8733..>8891
                     /locus_tag="SDD03_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG4304774.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDF"
BASE COUNT         3373 a         1119 c         1658 g         2741 t
ORIGIN      
        1 aaacttacaa gagaggggag taaagcagtt gcagaggaat caggaagatt tgttcgaaca
       61 gaaacttgca agaaagcatt attaaattga aggtgatgaa atatgaagta tttattgcat
      121 ggctatttta ttgattattc tactcgcaat agtagtactt tagcgattct atgtttgctt
      181 catgaattga gagtggttac tagaaaacag ctatacaaac tattgaattt tgacagatca
      241 attagtaaac aaggagtaac gaatgcttta gtgagattat atgaaaaaca gttaatcgat
      301 cgaaaaaaat atggaacaga aatgtattat tatttgacga aagagggaca tcaaagcata
      361 ggtgggtatt atacgttgcc gaaagttcct gaatataatt tgaatcacca tttacaaatt
      421 aacaattatt tgattaaagg aatggcattg gcaaaagacc acccacattt aaaacggatt
      481 atttcagaaa gacgtaccgt ctatgaaacg aaggatttaa atcctaagac aaaaggaaga
      541 aaattttttg tgccagattt tatttttcgt tttcgagaca aagaaaatca tgagatcaat
      601 tggtcctttg aaattgagct aacgttgaaa acacgtcgtc gctaccgtga agcgattttt
      661 ccgaaatata ttcgtgagtt gcgaacacgt cctgaatcac atttaattta tgtgacacct
      721 agtggcgcaa ttcaagaaga acttcatgcg tttaagcgta tttttttcaa gcgcgaaact
      781 gaaaaagatg cgagtcaaca agaattgttt gagcggttgc atattttttc agctttagag
      841 tttgaagagg gattaaaagc attattagag aatgatccag cagtcaattg gcggacagta
      901 gaacgagaaa aaatttatcc gaatggcgct gttcaaagtc gtttgaattt atttgaagaa
      961 tcaactgaaa atgaggaaaa accaaaagtg aagtttacgc ctgaagtatt gtaaaagagg
     1021 gtgttcgctt cgctcttttt ttgtgccagt ttgctggcac cgttcagacg caagcgtctg
     1081 aaccggctcg tttttaggaa ggactaactt gtttgattta tccgttgtca aagaagtgga
     1141 cgttcgagag tccctaatcg tatagcagtc attcggcgag tataacctaa aacataacct
     1201 gccaattgcc aagggtaaaa tgcacgctgc gcgcccttgg caatcgtcag aacatgtttt
     1261 cgagccggtt atgccgaacg accgctatac gaaaagggcc tctaaacaga agtccacttt
     1321 gatccttgac aactgaaacc caaaatcaaa caagcaggcc ttcgtctaaa aacgagccga
     1381 atgaagacct tgggggttgc acccccaaac ccccgtgccc ccgacacact accgaaaagg
     1441 tgggggtggg tggcgaaaaa atcaaataaa acattctatt caagaaagga agaaacaacc
     1501 atgacaacta aaaacgagca actgaaagaa caggcaagaa aagagttgca agaaagaggt
     1561 ttgattattg aggggagctt tgaaggagat tttgaaacct atattggttg ttatgcaaga
     1621 ccgataaaca aacctacagc cttagaccca atgaacgaac aagaagtaaa agaacaagaa
     1681 aaacatgcag tgaatggttt tccgcaagat ttcactgaat ggtatgagtg ggaaattaac
     1741 aacgggaagc ttgaaaactt cttataaaag tcagatgacg tgcaaagaga atgattctct
     1801 ttgtacggta aaaatataaa gaaagaagga aaaagaaaat ggcaaaaaac acaccttatc
     1861 aagtaggaga cattttttat agtagttggg gctacgaaca aacgaacgtg actttttggc
     1921 aaattgtgaa gttaacagaa aaaacagcat ggtttcagcc tattcaaaag aaaacggtcg
     1981 agcattattc gtccatgagt ggaaaagttg ttccaatgaa agacaagttt atagagtatc
     2041 ctttagcaaa aactaaagac agtattgtaa aaaaacgaat tgatccagaa acctatcctg
     2101 atatgttatg tggttatcgt agttgggaca cgtttcatag atataaaaat caagctgttt
     2161 atgaaagtag ttattattaa ggggatagtt tcccctttca aaaataataa atagaagaat
     2221 ggagagaaaa aatcatgatg aattcagtga atttagtagg acggttaaca aaagaagtgg
     2281 atttaaaata cacaccaaaa ggcaatgcga caggcacatt tattttagct gttaatcgaa
     2341 attatacgaa tgcaaacggc gagcgtgaag cagattatat ccgctgtgtc atttggcgga
     2401 aagctgcgga aactttagca aaatatactg gaaaaggaag tttgattggc attaatggac
     2461 gtcttcaaac gaggagctat cagaatcagc aaggacagac catgtatgtt acggaagttc
     2521 tagtgacaga tttctatttg ttagagagta aagaaatcaa tgagcaacgg tcaaaatcaa
     2581 tggcgaataa tcaaaataat tcttcaattc caggaaatga gattcaaatt tcagatagtt
     2641 atttgccctt ttaagtgaaa aataattttg ttctatacct gatttcttca ggtatagaac
     2701 aaaaaataaa tagagatgag gcgatttttt atgaaaatca ttgacagtta cgatcaacga
     2761 accatgcaat atttgggctc gtttgattat acagatgaaa atattattaa ttatgttgca
     2821 agtttacctc cttatcaaag cgttcgcttg gtagattttg caagtgatga attggtatta
     2881 acaacgatgg gcaacttttt agattatgtc ggagacaaaa aatggttgga aaaaattttg
     2941 tctaaattga ttgcgaaaca aacaggagaa gaacccataa atgcgattca attgttcaat
     3001 cgatatgaac aagaaaacaa tgattaaaga ggagagaaaa aatgaaaaga ttaaaacgaa
     3061 gtatttttat tgtgctagca ttgattgatt ttgttgggac aggttgtgaa gcaattgacg
     3121 ttcaaaaagc tcccttatcg gaaataaaac aagcaataac aaaacaagat gaagaacaac
     3181 cgctaaatac acaagtagaa atagtgaatc aaaataaacc gagtttttct gaagaagatt
     3241 tttcgttaga aaatggaacc aggcaatttt tttcagattt ggatcgttta aatcgggtag
     3301 gtgtagcaag tgcacttttg cataaaagca ggatgccaac agaagaacga gaccgattat
     3361 acattaagcc aactggctgg aaacaaaaga aaatgaagaa tggcgattat ctttataatc
     3421 gttgtcattt gattggctat caacgtgcga caagtttccc cataagtatg taatacgaaa
     3481 cgggaggatt aatcttaaga ttaatcacaa ctaacctatt tgacagttgg aatgaaagct
     3541 atcatgtctg ctgaatcaac tggtctgata ctcctacatg caataaagtc tactaggaag
     3601 ttattgtatg aagctaggta aaggcggcag aacaatttct aagtgtggaa tcatatgaag
     3661 ttggatatgc cgggacgcta taaattactc agggtgagac tgtttttaat taaactgacg
     3721 aacttgcgaa tgtacgggtc tataaccgac ttatattcga aagatataag attgcgaatg
     3781 caaagaacat tatcgaaaag taagaatgtt ggtcatgaaa ttcgagatgg aagactagcg
     3841 gtgtatctgt caagagttgg taaaacatgt ttgattgatt cagatgagag tgggaatgcg
     3901 acaaaacgtt ttacagaaga aatagagcaa ctagcagaac tgaatatacg atatttttaa
     3961 atactttagc ggagaataaa aaaatcctct acagaaaact acggagggca acatgtgcta
     4021 ttgtttcaac aatacatttt taatttttac cataacaaaa aagtacttga aaaaaaacag
     4081 aaatatgtta aattaaaaat gaaaaaatgg aagcggttac ttattctttt gaatctaaga
     4141 taaaaattca aaagaataac ataacccaaa tatgattata cttgagattc taaatattta
     4201 tcgaaaagga atttgtttta aggaaaaatg attctatgga cggttattga tttatattta
     4261 ccaaatggct aataaaatag tatttcttat attgtatagt ttttgttttt atgtgaaaac
     4321 atgagcaata gaattgtact agtaggataa ggatggatgg aaaatgaaaa aaactatttt
     4381 tgttttagcg atttttatat gttttgggct acttttggta ggctacttgt tgtttaattc
     4441 taatgaaatg agaactattt ctacgaatga cttgttaaaa aaaagtgaat taaaggataa
     4501 aaaaaaagaa tctcttgcct ttataatgtt tactcaagaa gagtgtgagt attgtatagc
     4561 aataaatcca atcatagata gtgtaaataa ggaaactttg aataagataa agaaatatga
     4621 tatatctgta gattttcaat ctaaacaaaa gtgtaaaatt aagtttgttc cgactattgt
     4681 tgcatatagt aagaataaag aagtggggag aattgacaat gaattagata atttgtatcg
     4741 aaaaaagcaa gatggttcaa ttgtaataga caagaaaaaa acaaagacat tgcttataga
     4801 ttggatgaaa aaaatggata aacaaggaga atatttgtat ggagaagatt aaacagacat
     4861 ttccgttaat taataaatat tggaataaga aaaaaatttt tatatttttt attacgtttc
     4921 tgctgtcatc tttattttct ctaattttcc cgtatctatt tagtaatgtt attgatttat
     4981 atttagagaa tgagttatca cagaatattg tgcgaatagt aacgattctt atatttcagt
     5041 tatttttagt cttaattaaa gaagtgtatg gatatataaa aactatttat gcatttgaag
     5101 ttgatttatc aatgaaaatt gattttctta aggttttaca aaaaaaaaat cttttttttt
     5161 cgcaaacgaa gcattcagga gaaattcaat ttaggatgtt ttcggatatt tctactatcg
     5221 taaatactat ctttattttt tctgttgaat tacctgtgat aggcgtttta gttcttgtag
     5281 cggtatttat tataagtaaa gtgaatctta tttttctgat tttttcacta ataatgatgg
     5341 gagctatggt gttacagtat aatttatttc aattaccgct taagaaaaca atcgagaata
     5401 caaaaagtgt taatcaaagt attacaggag aattgaatgc acattttgat caattagaaa
     5461 ttataagcgc atataatttt gagaatatct ctattttaaa attcgattct agatttaaag
     5521 aattatttaa tgttttcaaa aaacaaaata aattactaac actgggaagc tgtttggcac
     5581 aggttatttt ccaattatgg attggaggaa tatttcttat tgatgttatt ttattagcta
     5641 aaggaacaat aagtattact aattttgtaa tagtatttat gtacgctaac atggtcccta
     5701 ctttatggac tactttaatt agtattattt ggaaatggca agactattca gtgtcattaa
     5761 atagatatta cgaatattat tcaaataaca acaaagtggc tggcacaaaa aaaataaata
     5821 aaataaattc tattactggt caatcattaa atttttcata taacaaaaga aaggtattaa
     5881 gcaatataag tttttcaact gaaatgaata aactattcat gatatcagga gaaaatggaa
     5941 cagggaaaac aacactgatg aaattattta ctaaacagtt gccggttagt aataatcaat
     6001 tattcgtcaa cgggatagat attaatatga ttgataacga atcattaaga aggttaattt
     6061 caatcgcaac acaaatacca tatataatta atagctccgt gaaggagaat attttaatgg
     6121 ggactaaaaa ctatgataaa aaaaaattca gtaaattaat agaactattg ggaataagta
     6181 aatttatgac gaaattaggt attactttgg acgacaatat ttatgatgaa aaaatattat
     6241 tatctggagg agaaaaacaa aaaataagtt tagctagggc gcttattaaa tcagcttcag
     6301 ttattatatt agatgaacca tttagaaatt tagataatga aactcgcgat aatttgaggg
     6361 attatatact aaatacaaaa aaagaagttt gttatataat tataactcat gatcacattc
     6421 tagataattt tttggattca aatgatgaaa tattatattt aaataaggag tatgataata
     6481 atgaatagca aaaaacttca gatgggtgaa attgttagag gtcctagtca aatatctatt
     6541 gatgttacat ataaatgtaa ttttagatgt aaacattgtt acaatgcgag tggtggagac
     6601 aattgtttta tggataatga gttgactgat gatgaactgt tagaacttac aaaagatata
     6661 tgtaatttaa agccatataa tatttgtttc tgtggaggag aaactttact gagaaaaaaa
     6721 atagttttat cttgtgccac tttattaaaa gaggcaggta ttcctaatat tgctattgtt
     6781 actaatggat cattagtaac tcaggatgtt gcaaaagaat tattcaaagc aggtattaat
     6841 cgatttcaag taagtttaga tggaaaaaat gctagtagcc atgaagcact tagattatat
     6901 ccaaacagtt ttgaaaaggc aattaatgct atcaaattat ttagagaatt gtccgaagaa
     6961 gtggatgttg ctttttgtcc tactagtttt aattacaatc aattacgaga agtatatcaa
     7021 ctagtaaata ctcttaaagt gaagtcttta agaattcaac catttatgct tagtggtagg
     7081 ggaatgcaaa atagggaaat agtgccaact gtttctcaat atagaaaact agtttctgat
     7141 attaactata ttaaaagaaa tgaagattat accaagatag attggggaga tccaattgat
     7201 catatcatcc gacacagaac tttattgaaa gatttttcga attttgtttc tattaggtca
     7261 gacggaggat taatggcatc accttacttg ccaattcaag ttggaaatat taaatgtcac
     7321 agtctttcta aatattggcg agcaggactt tataaagttt tttctttaga tttagttcaa
     7381 gatatagcta ctcaagtttc ttcaatacaa gatatgagta aagaattaaa aaaaggacat
     7441 attaaatggc ttaatgaaga tatagaaatt gatattctcg atgaatacaa acatgatatt
     7501 ttaatttagg tttatcattg gaaagtttag ataaacaaag tacttttaga aaggagggaa
     7561 aaaaatgatt tctgaacctc aaatgaataa tatgtatgaa agcccaacta taatgggagc
     7621 tggtgaagct gcgccaagag cttgttcagc aattatggtg tgtgtgttat tagcatctgc
     7681 aggagtaggt atccaagcag gagcggtgta tacttatgct gtagttgcta cagtagctgt
     7741 agctgcagcc gcagtttgga aaacagtagg attagttggt taggaattaa taggagacta
     7801 actgtctcct attgattcca atgattaagc taaggaggaa ttttaagaaa ataatgaaat
     7861 acaataattt aaaaccgaaa ttacttaatg agaacataga atatataaga gaagaaaatt
     7921 atggatattt aaccatagtt cccaaaaatg gaactgaatt gaaagataac gttttcaatc
     7981 caacagtaag atatatattg gctaactgta ctggaaaaaa cagtgtaaaa aaaatagcga
     8041 atggtttaaa aaagaagttt cctagtttag aatatgaaaa attctatcaa gatgttgtag
     8101 gaactattaa atttttcaaa cactataata ttattgatat aggagattat agtatggaag
     8161 aaaagaaaat atataaaatt gaacaaaatt tagaattaga attactaaat gaatcacatt
     8221 atgaagaaat ttataatttt atcacagatc atcctgaaag tatgtatttt catgaagata
     8281 tttatcatca aggaaatagt aaagaattag aagttgtcct tagatataat ttattttcgt
     8341 ttacgagtga attttacttt ttaagacata aagagagaat tgtaggaatg gtttctataa
     8401 ttgttaatcc atttcaaaac ttatcctaca gtatagtaaa ttacctcata tgtgagcctg
     8461 catatactac gactattttg acaacagtat tagaaaatat taacgaagtt tcaattttag
     8521 aagtaaagaa gctagtgatt catttgctaa attcaacaga agatgaatca ctaatcccct
     8581 atttaaaaga ttcacatttt tttaaagaag cggtactatc taaagaatat agagagcatg
     8641 atgttttgag atacgcttac atttattaaa taaaatttct gatagtattt taataattcg
     8701 gatcatatca taagatatag gagggaaaag aagtgatttt attgattttg tctctcgtat
     8761 tactagttgt atctaatcta atgaaaaata atcctcctac aaaaattaat tctttttatg
     8821 gctttagaac taaaaagtca atgaaaaatg aaaaaagttg gcatttagca caaaaattgt
     8881 tgggggattt t
//
LOCUS       DARDHU010000100         8800 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000100 DARDHU010000000
VERSION     DARDHU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.101"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="SDD03_002347"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="SDD03_002347"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG4304775.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="SDD03_002348"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="SDD03_002348"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG4304776.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="SDD03_002349"
     CDS             complement(3072..4325)
                     /locus_tag="SDD03_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG4304777.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="SDD03_002350"
     CDS             complement(4331..5254)
                     /locus_tag="SDD03_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG4304778.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="SDD03_002351"
     CDS             complement(5475..7967)
                     /locus_tag="SDD03_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG4304779.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="SDD03_002352"
     CDS             complement(7974..8441)
                     /locus_tag="SDD03_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG4304780.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8577..8650)
                     /locus_tag="SDD03_002353"
     tRNA            complement(8577..8650)
                     /locus_tag="SDD03_002353"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8614..8616),aa:Arg,seq:acg)
     gene            complement(8708..8781)
                     /locus_tag="SDD03_002354"
     tRNA            complement(8708..8781)
                     /locus_tag="SDD03_002354"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8745..8747),aa:Pro,seq:tgg)
BASE COUNT         2441 a         1867 c         1461 g         3031 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
     8581 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
     8641 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     8701 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     8761 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DARDHU010000101         8675 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000101 DARDHU010000000
VERSION     DARDHU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.102"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1101
                     /locus_tag="SDD03_002355"
     CDS             <1..1101
                     /locus_tag="SDD03_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4304781.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="SDD03_002356"
     CDS             complement(1280..1609)
                     /locus_tag="SDD03_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG4304782.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="SDD03_002357"
     CDS             complement(1599..1871)
                     /locus_tag="SDD03_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG4304783.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="SDD03_002358"
     CDS             complement(2322..3125)
                     /locus_tag="SDD03_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4304784.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="SDD03_002359"
     CDS             complement(3103..4227)
                     /locus_tag="SDD03_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4304785.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="SDD03_002360"
     CDS             complement(4243..5058)
                     /locus_tag="SDD03_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4304786.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="SDD03_002361"
     CDS             complement(5048..5920)
                     /locus_tag="SDD03_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4304787.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="SDD03_002362"
     CDS             complement(6005..7294)
                     /locus_tag="SDD03_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4304788.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="SDD03_002363"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="SDD03_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DARDHU010000102         8495 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000102 DARDHU010000000
VERSION     DARDHU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.103"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..174)
                     /locus_tag="SDD03_002364"
     CDS             complement(<1..174)
                     /locus_tag="SDD03_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4304789.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFLM"
     gene            complement(594..1715)
                     /locus_tag="SDD03_002365"
     CDS             complement(594..1715)
                     /locus_tag="SDD03_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG4304790.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2043..3452
                     /gene="istA"
                     /locus_tag="SDD03_002366"
     CDS             2043..3452
                     /gene="istA"
                     /locus_tag="SDD03_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG4304791.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3449..4177
                     /gene="istB"
                     /locus_tag="SDD03_002367"
     CDS             3449..4177
                     /gene="istB"
                     /locus_tag="SDD03_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG4304792.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4305..5216)
                     /locus_tag="SDD03_002368"
     CDS             complement(4305..5216)
                     /locus_tag="SDD03_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4304793.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5233..6240)
                     /locus_tag="SDD03_002369"
     CDS             complement(5233..6240)
                     /locus_tag="SDD03_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG4304794.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6237..8366)
                     /locus_tag="SDD03_002370"
     CDS             complement(6237..8366)
                     /locus_tag="SDD03_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4304795.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8371..>8495)
                     /locus_tag="SDD03_002371"
     CDS             complement(8371..>8495)
                     /locus_tag="SDD03_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4304796.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1729 c         1486 g         2681 t
ORIGIN      
        1 cattaaaaag ttagtaaata attgccctct aagttccacg gtctgttcat taataacttt
       61 atatcctcgt ttttcaaaaa atggctttgc ggtaatggaa gcgtgggtag tgattttttg
      121 cataccagtt tctaaaacat ggttttccaa ttcatctgca attttagtcg caataccttt
      181 tccttgatgg ctatgttttt gtcacaactt cagtattcct gtaacaatac ggatttcagt
      241 atacaacaac agcttttgaa aagatacttg caaaaggatt aagatatcta tgacctgcta
      301 tgctcactga tgttgaaaag aatgttttat taggattgag gtaagcaaga tagtgaatat
      361 gtccggaaag cctgtaaaat aaggcttttc ggacatatgt gaagttataa aaagatagcg
      421 aaaaatctat ttagtatcac taatgttgcg aacatatcgt cagaatagtc tgacatttat
      481 ttcttgctaa aaatttcaaa aaagtcaagt gctgacgcag cggatgacta ttttccagaa
      541 aattttagta tgtattcaaa aatcaaacgt gacaacagct attaactaca tcctcatcca
      601 ggtctgtaac gtttgaacca ctcttctgcg ggctcaaaca ttaagtaatg gaaggtacgt
      661 ttcaattgaa acagcgtcgc tgtcgtattc aactcaattt ttatcacata agtgagaagc
      721 gttgcgatta aggtgaggat gacttgattt atcgcccctt gttcactatg tgaatatagt
      781 cgtttaatgg tcagattttg ttttaagtgt ttgaacaaca attcaatctg ccaacgggct
      841 tgatacatgt cggccacttc gtttggcgat acatcaaaga gattggtcac cattcgcagg
      901 attttttgtc ccttggtaag aaccgttacc aacctaaaac gctctgtcac atggttgaca
      961 ccgccgacaa gaatcacttg ttggtcactg attatcgtcc catcacgggt tgttgtatct
     1021 gctacttcaa tttgatccaa gatggtaatt tttgtattag attttgtccg tgtaacgaag
     1081 aaatagccgt cacgatttag tttgtctaac aacttgtaat caaagtagcc gcgatccacc
     1141 acatacgttg cttgcgtttt atcaacaaga acttcaaggt gattacggtc atgctcactc
     1201 gcatttgtca tcgtaaaact ttcaggatac tgattggttt tatcaagata acagagattc
     1261 aaatgtagct tgatgccaga ctttgttttc cgaaacttgg cccatggaaa ccatgctttg
     1321 ttcaacggaa tagtcgttga atcaatcaat tgtagactgg ttgtttttga gcttggctgc
     1381 tgagcagaaa ttctggcgac taattgcgta aaaatttcca tcaatacttc ttgcttgatt
     1441 tctggcgctc tacgagataa cgagctgtaa cacagggaat caatgccaag ttccttgcag
     1501 aggcgctgat ctaaaaaggc gcgatcaatc tcgcgatagc ttggcaattc ttcataaacc
     1561 gcatgaagta acactttaag cgtcgtttgg aagtctagtt ttttactgta gaaatcaaag
     1621 tcgctaatta agctttgaga gttttctgat aaaccagaaa aatttattgc agaaaaccat
     1681 ttttgaaatg aggtttttac gttatactta tccatgcgtg agtccttttt attggtcttg
     1741 gataagtcat ccgagaatag tataaaggac ttttttgcgt ttggatagct ttgtattagg
     1801 cttttgatgg gttaaaaagt ctttgcaaca ctagtaattt agtatttatg tataaattga
     1861 aagatatttt accaccactt agtcttctta aaagtgatcc aacattttgt ttgtggcaga
     1921 atttaactac acactaatgc agccgaaagt acacaactat gcagcagttt gactgcgaac
     1981 tggtttcctt ccatcataag tttgaatgac ttagtgatgg aggtacagaa aggttgttaa
     2041 ctttggatga tattaaaaca ataaaacatt tgagaaataa tcgtgatcag tcaattgatc
     2101 agataagaaa agcaatgaat atcaattggc gaacagcgaa aaaatacgcc gatagtgatc
     2161 aactattcat tacagatccc actcgaaaaa acaaatccat gatggatgat ggttggggag
     2221 aaatcatcgg tagttggcta gaagaagacc tacgtttacg cagaaaactt cgtagaacgg
     2281 ccaaagctat ccatgaacaa ttgaaacctt tgggtttccc tggctcctat cgaacagttt
     2341 gtgtatacat ttctcaatgg aaaaagcagc gttacgacga aacgaattac caagctgtcg
     2401 atcgtcttga tcatccgatc ggagaagcac aggtcgattt tggaacaatg gaagttatgc
     2461 atgaagatca catgaaagat atacaacttc ttatcatgtc atttccctat agtaatgcga
     2521 cgtccgcaag tgccatgcca gctcaaaacc aagagtgttt cctggaagca ttaaaacaac
     2581 tatttaatca atgcggcggc gttccaaaaa cgatcagaat agataactta actccagcgg
     2641 tgaaaaaaac aaaatcaaaa tttgaagaag cacaactaac cgatgaattc ttacgtttca
     2701 ccattcatta tggttgtgat gttcaagtct gtaacccacg tagtgggcat gaaaaaggac
     2761 atgtcgaaaa taaagtgggt tatgtccgat acaatttctt ctcgacttcg ccaatcatgg
     2821 actcttttga gtccctaaat aagcaacttg aagaacaatt aaaagcagat cgaaaaagga
     2881 cgcattattc aaaacatcaa ttaatcgaag atttgtggaa gaaagaaaat aaagcgttac
     2941 attctcttcc acaagaggat tacccaattt ttaaagaagt ggaagctaaa ctaaatcgtt
     3001 atcacgaaat gaaattggat caaacggtta ttcatattcc taaagcatat ggctattctc
     3061 aattgactgt tgttttgact tggaataaat tcaaagttct tacgcctcat ggagaaatat
     3121 taatgaacga atatcgcccc tatatgaaca aaaaaagatt gatcccatgg agtgacatca
     3181 tgaaacaatg gagtaataaa ttgaatgtcg ttagttattc gaggtattgg aagtacttgc
     3241 ctgcacgcct tcaaaattat ctagcgattg ataataatca cctgaaatac agacggataa
     3301 aacaattact aagtttatta gcgacctacg atatccaaca aattaatgaa caattttacg
     3361 aattagtcgt tcaagaagac gccccctctg actttgatgt gaattggtca aactatgatg
     3421 cattaacaaa aacaggaggt gtgcaaaaat gattggacag acgttagaag attattgtaa
     3481 aaagcttcga ttaagccata tcatcacgaa cataccaaga gcttctttcg aagctccaga
     3541 acaatttatt accgatttat tccgtttaga actggagacc agacagcaga tcaaattaga
     3601 acggatggaa aaattagcca aatttcctgt accaaaatcg ctaaagaact atgaatggca
     3661 cgaagatatt tttctaccat ctcatctaga taaggagacc cttgtctctc ttgattttct
     3721 aaaaaggcaa gaaaacattg tttgtgttgg ctctcctggt acagggaaaa cacatctagc
     3781 gatcgcatta gggagaaaag cctgtagtga aggtgtttca accaaatttt tccgagttaa
     3841 caatctcgta gagcaattag agaatgcatt aaaaaaacaa cagctagcca gtttcaagaa
     3901 gcgattcgaa acaacagaat taattattct tgatgaaatg gggtacttac cattgacaaa
     3961 agaaggagct gaacttctct tccaactgat ttcagattgg tatgagcaaa agagcattat
     4021 tattacctct aatttagaat tcagtcaatg gaatcgagtg tttatggatt ctcgattaac
     4081 agctgctctc gttgatcggc taatccatca cgctcacatt atcagcttca aaggcgaaag
     4141 tttcagattg aaaaattcat tatctaaatc atattgaagg aaaaaactag ttgcagcaaa
     4201 actctgcact ttcggctgca tttttttgta aaaagctatt gcacaacaca aacatttgaa
     4261 cgacgtaatt ccaaatgttg gattgctttt agataatctt ttatttattc acaattgcta
     4321 atagtccaat tttcattttt gtatagtttt aatctatact gaaaaacact tgttgttttg
     4381 gacaactcat caagatactc aacaccaaca atcacttgaa tcgctttacc cttttgtttg
     4441 taaataggat tcactaattc cacaaatttc aaattagtat taattggttg catcgcgtct
     4501 ttttcaacat aatgctctag ttctttttct gaggctgctg gataaagcct gaaaaacgtc
     4561 tctaaaaact cggtgatttc ttccgttgtc tttgaatcaa tttttgaatc actttttact
     4621 tgtcttactt tatgttttgc cttttctggc ttaccaatca tagtgggtaa actcgttaca
     4681 atatgattgc ctttttcatc ttcaaagatc atcagacgat atgcagaaga aattgattgt
     4741 gtctttttcc cttctgtaat cttttgatct accacaaatg aaacgacaaa ttcttccgag
     4801 tgtttttcaa catctagaat ttttactgat acaacctcgg aactagtggg aatatccgct
     4861 cgaaccatat cttggctcaa agcgagccct tcttctgtaa ggtactgctc aagattagtc
     4921 attctctttt caattacctc cttgttattc ttccatgaaa aatattcttt cacaaaatct
     4981 acagtaaaat tttctactcc agaggtattc actattttct ctttcacgac cactttctca
     5041 tgagttgtat gctgatcaat ggccgtgaag tttttgtaga ttccaaaagc aaggctacaa
     5101 gacaataaaa gccaaagggc aagcaccatt tttcgatgct tgcccacgga tactactcgt
     5161 tctttcttct tttttggaat cttttccttg ttacttcgct caatttttat cttcattttc
     5221 cattcctctt ttctattgtt tataccgacc agcaccaata atgtgctgtt gccaatagct
     5281 atcggtaaga ttggtccaac ccaccggatc tcctgcatga aacattctgt tctctcctgc
     5341 ataaatgccc acatgagtca cataagtacc ggcatcataa gttgaatgaa agaaaatcaa
     5401 atcgcctggt ttggcatctt ttaagtcaat atgagtcata gcatcatatt gatcctgagc
     5461 cacccgaggg agtttcaaac caactttggc ataacaccat tgagttaaac cactacaatc
     5521 aaacccagtt ccgggacttg acccaccaaa tacataagcc gttccttcat acttgaacgc
     5581 ctcgtcaaaa attccttgga ctgttttatc atcaaactgt gttgtagtta ggtactgctt
     5641 cactaataag caataaaaca tattgccata gttataccgc cacccactat tttccttaat
     5701 cgcgatggga ttgggataat cgactttttg accacccgat ttttcttttg aaaaagattc
     5761 ggctaaagca aaactatgct tctttccgtt tttcgcgaca taatccaaat acccgtcacc
     5821 atagttataa gcctgaatga tactatcttg atcgcatcct agttctttgg aacacttaac
     5881 caaagacgaa aaatagagac acacttgttt gatggatgcc tcggtactta atgaattcgg
     5941 tgataaacct gctgattcag aactttgcat gacatcttcc attgtacctc cagattcgac
     6001 ttgaataatc gccaaaagaa tcgaaacata ctctgttata tctgattctt tggcatactt
     6061 ttccaccatt gaacggtgtt tcaaaactac ttctgagaca ctactacctc caggagaagt
     6121 tccagaacta ccacttccct caccgtcctc accaaacaac aaacccacac acatcaagat
     6181 gcccacaaaa gagatggtca ggaaggttaa cgaaataata ctcaattttt ttaagttcat
     6241 cttttctcac ctggctttgg ttttgggcga gtgttctttt ttctttgtac ttgttgattg
     6301 gttcgctttg gaaaagtgaa tctctgtttc acttgttgaa aggaattgtt cttgcttagt
     6361 ggctgagtgc ttccgtttgg aatgacttct tgactagaaa gtttccgttt gatttcagga
     6421 ttcgaatgta cttgtaccct tggttttaaa gacctatttt ctggtagttc atttttctgt
     6481 ggttttatct ccttttttat cggaacattt tcccttcctt tctcatgacg attccatgct
     6541 tcgttcaata ctttgcgttt ttcatccatt tttgcgtctc ttcgttgtcg ttgtttttct
     6601 cgcaactccc ctcgatttgc tttttcttcg aaaggccctc gcttaaagtc ctctggtact
     6661 tttttcgtct tttccatgcc tttatgaagg ctgtatttca agttagttgg tgcatcggtt
     6721 acttgatttt ttgcttgttt cgctttgtcc ttcacgattt cttttgtatc gagtactttt
     6781 ccaacttttc taccaactaa atttattcgt gattctttct tcgtgcccac tggttgttct
     6841 tgttttttca gatcagaatt tagctcgtta atgcttctcg cggaatggtt ctttctattg
     6901 gacactctat tgatttttga actacctttc ccttttgtag aactggtagc tagtttccca
     6961 ccaactgctg caccgccacc aaaagcaaaa gttcgaccca catttcgatt aagctgtcgc
     7021 aattttcgat tcatcaacat ttgcggtttc cgcatgactc gccttcccat tgactgacta
     7081 tcattacttt gaaggttaaa catgctcata atgtcaccta atttgaaata gataccggca
     7141 aaggtcacaa tttgtagaaa agctaccata aagaagggat aggtggccga aatattgaaa
     7201 aacatcgtgg agatactgaa tgcagtcgta atgactaagg taactcccgc tcgcatcata
     7261 atcgtattga acaaacgaat aatcgccctt ttgcccaaac tctcatacgt cggcaacatc
     7321 gacaataaga aactaatggg cagaaacatc gcaaagataa tgaaaaggat ttgtgagaac
     7381 aacataatcc ctgtcagaag aaacacaaaa aaggaaatcc caatattaaa caacactaaa
     7441 aagaccacgg ttcctaaacg gttcatggtc ttggtaatac tgagattcgc attgtcatta
     7501 tcttcaatct ctgctttgac tgcttcttcc cgatccttac ccttattttc atccggactc
     7561 acggataaaa tcttattaac ccgaccctcg ccaatttctt ctatattgga atcatcgaat
     7621 tgtaatagta accagggttg ttgaacttga atcgaaaata aactgtctcg aatcaaatcc
     7681 acactatctt ttccctggct ttctgaatta ggtacaacaa tcttggttcc aaggctaaga
     7741 gccgcttcac taacgtctga gctaaagtca ttgatttttg taatataggt gggcgcatac
     7801 gcaatgaaag aacccgagag tagaaaaatc atcacaaaat taatgacggt tcggactgct
     7861 ttggtagttt ctcgttttaa taatccggta tacgccacat aaactcctag agctaaaatc
     7921 atcaataata ggaacccaaa gtagaatccc gaagtctgta atccattttc agtgataccg
     7981 gctaaagttt ggatattctt tccaatactt ttagccgtat ccgaaatgaa atccaacttg
     8041 taagcttctt gaaccacata gcccgtggcg tttgataaat acagacttac tgtccaaata
     8101 aaattggtaa tggcataaag accatacatc acgcttttcc ctaagccatc cccccaattc
     8161 caaggtaacc aatcccaaga actatccaca aagaaatcca gttgataatt gttcagagaa
     8221 tatttggaat aaaggtttcc ggcttcaact gtatcatcaa ccagacctgt cgcctcagct
     8281 accgtaccga ctaaactcaa tagaactgtc agagtgacga tgctaataaa aacgattccc
     8341 acaatccgaa aaagtttctt tttcatcccc tcactccatt ctctcttgaa ccggcggacg
     8401 agtgtcaaaa gcatggaaga gttcttcaaa gactggatga acttgaacca cacctactcg
     8461 cccatacaag tcttgcatca agcattgacc gtttt
//
LOCUS       DARDHU010000103         8458 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000103 DARDHU010000000
VERSION     DARDHU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.104"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1118)
                     /locus_tag="SDD03_002372"
     CDS             complement(150..1118)
                     /locus_tag="SDD03_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG4304797.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1350..2183
                     /locus_tag="SDD03_002373"
     CDS             1350..2183
                     /locus_tag="SDD03_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG4304798.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2217..3056
                     /locus_tag="SDD03_002374"
     CDS             2217..3056
                     /locus_tag="SDD03_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4304799.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3082..3642
                     /locus_tag="SDD03_002375"
     CDS             3082..3642
                     /locus_tag="SDD03_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4304800.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3841..5562
                     /locus_tag="SDD03_002376"
     CDS             3841..5562
                     /locus_tag="SDD03_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG4304801.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5727..6869
                     /locus_tag="SDD03_002377"
     CDS             5727..6869
                     /locus_tag="SDD03_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4304802.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6885..8096
                     /gene="thiI"
                     /locus_tag="SDD03_002378"
     CDS             6885..8096
                     /gene="thiI"
                     /locus_tag="SDD03_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG4304803.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2905 a         1459 c         1757 g         2337 t
ORIGIN      
        1 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
       61 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
      121 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
      181 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
      241 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
      301 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
      361 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
      421 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
      481 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
      541 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
      601 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
      661 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
      721 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
      781 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
      841 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
      901 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
      961 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     1021 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     1081 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     1141 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     1201 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     1261 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     1321 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     1381 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     1441 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     1501 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     1561 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     1621 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     1681 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     1741 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     1801 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     1861 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     1921 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     1981 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     2041 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     2101 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     2161 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     2221 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     2281 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     2341 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     2401 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     2461 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     2521 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     2581 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     2641 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     2701 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     2761 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     2821 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     2881 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     2941 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     3001 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     3061 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     3121 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     3181 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     3241 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     3301 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     3361 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     3421 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     3481 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     3541 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     3601 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     3661 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     3721 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     3781 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     3841 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     3901 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     3961 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     4021 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     4081 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     4141 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     4201 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     4261 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     4321 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     4381 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     4441 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     4501 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
     4561 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
     4621 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
     4681 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
     4741 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
     4801 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
     4861 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
     4921 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
     4981 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
     5041 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
     5101 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
     5161 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
     5221 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
     5281 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
     5341 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
     5401 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
     5461 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
     5521 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
     5581 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
     5641 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
     5701 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
     5761 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
     5821 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
     5881 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
     5941 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
     6001 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
     6061 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
     6121 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
     6181 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
     6241 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
     6301 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
     6361 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
     6421 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
     6481 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
     6541 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
     6601 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
     6661 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
     6721 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
     6781 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
     6841 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
     6901 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
     6961 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
     7021 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
     7081 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
     7141 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
     7201 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
     7261 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
     7321 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
     7381 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
     7441 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
     7501 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
     7561 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
     7621 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
     7681 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
     7741 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
     7801 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
     7861 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
     7921 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
     7981 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
     8041 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
     8101 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
     8161 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
     8221 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
     8281 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
     8341 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
     8401 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DARDHU010000104         8276 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000104 DARDHU010000000
VERSION     DARDHU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.105"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            56..529
                     /locus_tag="SDD03_002379"
     CDS             56..529
                     /locus_tag="SDD03_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG4304804.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            538..765
                     /locus_tag="SDD03_002380"
     CDS             538..765
                     /locus_tag="SDD03_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4304805.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1401..1772
                     /locus_tag="SDD03_002381"
     CDS             1401..1772
                     /locus_tag="SDD03_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304806.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2028..2270)
                     /locus_tag="SDD03_002382"
     CDS             complement(2028..2270)
                     /locus_tag="SDD03_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4304807.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2302..3204)
                     /locus_tag="SDD03_002383"
     CDS             complement(2302..3204)
                     /locus_tag="SDD03_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4304808.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3217..3405)
                     /locus_tag="SDD03_002384"
     CDS             complement(3217..3405)
                     /locus_tag="SDD03_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG4304809.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3419..3982)
                     /gene="amaP"
                     /locus_tag="SDD03_002385"
     CDS             complement(3419..3982)
                     /gene="amaP"
                     /locus_tag="SDD03_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG4304810.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4020..4913)
                     /locus_tag="SDD03_002386"
     CDS             complement(4020..4913)
                     /locus_tag="SDD03_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4304811.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4991..5914)
                     /locus_tag="SDD03_002387"
     CDS             complement(4991..5914)
                     /locus_tag="SDD03_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4304812.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5963..6313)
                     /locus_tag="SDD03_002388"
     CDS             complement(5963..6313)
                     /locus_tag="SDD03_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4304813.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6346..7248)
                     /locus_tag="SDD03_002389"
     CDS             complement(6346..7248)
                     /locus_tag="SDD03_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG4304814.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7241..8098)
                     /locus_tag="SDD03_002390"
     CDS             complement(7241..8098)
                     /locus_tag="SDD03_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG4304815.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2272 a         1592 c         1399 g         3013 t
ORIGIN      
        1 tttacaataa ttattctgct atattttagc tatcaaagat aaaagaggga taaaaatgaa
       61 atttaaaaca ttgctattaa aatttgtcgt tttaggtata agtgccgtat ttattttgtt
      121 cggcgtactt ttttatactc agatcgctac aagcgaaaaa ggattcaatt ttgattggca
      181 gaccacatta tttgtttctg tcatttactt tgcccttctc ctagggatcg ttattgctta
      241 tttcttattg aaactattga aaaaaatcga agataaccaa gctttttcca ctcaaagctt
      301 aaacatacta aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct tttttggctt
      361 acttcctaaa tttatgaaag tagcggattt agacgatgct ccaggtttga tgttggtagg
      421 aatcgctgtc gtattcattc cttttgcagt atacacatta acaagcgtgc tggaaaaact
      481 gcttgaacat gcgattttac ttaagacaga tcatgatttg acagtgtgag gtgcgacatg
      541 gcaattatcg ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg
      601 agtgagaaag tgggcattac gatggcaaac atctctattc taaaaaatgg aaaagcaaaa
      661 gccatccgtt tttccactct cgaaaaaatc tgccaagttt tggagtgtca accaggtgat
      721 ttgttgacct ataaaaatga ctcgaatact ataccagaag aataaaataa agtagaacaa
      781 acagtaaata aaaaataatc tgaatgatag atgtgtgaga cttttagaag ttatttacgt
      841 ctattattca gattttttat ttcgattgaa taccgtttta tctataaata agatatgtac
      901 tgactcatac aaagggggcc ttttcttctc catgtactat cacttttcct gatacaactg
      961 gtttcctgtt acgttgtagt tggatctgct tttagtataa tactttattc attccaccag
     1021 actgatagca gtttgagcag tctaaataat catgtagtat tacaggccat tctgtttcat
     1081 accttgaagt gcttaaaact tcagctaatg gcccattgtg ttaactctta ctccattctt
     1141 ttttagtaag ctcccatata tactgtaaag ccttctatat attatgtcat gttgcttttt
     1201 aacatttact atgtagtttt gataaaatga tttgaataac agtgatggac aattcttttt
     1261 tatttttaac attattttga taataatgat tatttattta aaaatacatt agataacatt
     1321 tgttacgaca cgaaaaagct tcacaaacaa atatcgttat tttaaaatga gggcgaactc
     1381 acacaattag gaggaatagt atgccattta ttgtttactt ttttctttca ttactcgtga
     1441 tctatattcc attaccaaca tttttcctgc tcgtcaatcg tctagcagag caacatgata
     1501 cgattgctgc tcttacgaaa ctttgtttac ttctatttgt ttgtctagat tactttacga
     1561 gctattattg gctaaaacag acaagagtaa aaaagagata ttatttcttc cttgtctttt
     1621 tcaatctctt tgagtttctg atccattgct ttttacttca tcagtatcat acgatccatt
     1681 tgtggatcat ctgggttttc aaagtggtcg tattattttg tatgttcacc tttcctatct
     1741 caagaaaatt tagggaggca ttgttttatt aaagaagctt ctctgtatca ataaagacac
     1801 atttaaggaa aaacttttaa aaatctaatt gggaatttgc ccgcataaat aagaggctgt
     1861 gacaaaagtc ttgtcacagc ctcttattcc gtataaacag tgttcaaaag cttgcatctt
     1921 cgataatatg cccaaccaaa tgaaaaatat gagaagctat ttttgtttgg ctgttcttat
     1981 tacttgatga agaacagctt tttcacgacc tctttttaaa agtcttttta cagttttcga
     2041 gcaaagaatg atacgattag tactaaaatg atggcaccaa tcaatgatgg cacgattgcc
     2101 attcctgcaa tacttggtcc ccaagaacct aataatgctt gaccaagtgc tgatccaact
     2161 aaacctgcta cgacatttgc aataatcccc atcgaagaac ctttattcgt gattgctcct
     2221 gcaattgctc cgataattcc tccgacgata agtgaccaaa taaatcccat gtttcgttcc
     2281 tcctaatgtt ttacttcctt attactgaac ccgtggttct gtattttctt ttactttttg
     2341 tgcgccgctt ccagctgctg attttacttt atctgtttga ttcgaagcaa attcaccggt
     2401 ttttttcgct gcgtcagtta ctttgtcttg caccgtttct tgatcttttt caaattcttc
     2461 ttttgttttg atgtccacta cgttagcgtt gacttcgatc acttctaaat gtgtcatatt
     2521 ttttacttct ttgccgatta cttcttgcat ttcttcaaag attttttcga tatctttgcc
     2581 atattccaca atgacatcca tatcaactgc tacttgtttt ttaccgactt ctgtttcgat
     2641 cccagccgtt acattatctg tattcacaag ttttcctgcc acattagaga agaaaccacc
     2701 gtttgcagat aataaaccgt ctacttgttc aattgcgatt ccgatgattt tttgcacaac
     2761 tttatcttca taattcagct caccgcgcat tgaatcttcc tgttcagaat catgctttcc
     2821 ttggttattt gtatgatcag aaccaagttt tccttgtccg tttgtgtggt cagaactaag
     2881 tttttcttgt gctttcgcat ggtcagaagg atgtttttgc tcttccacag gtttagaagg
     2941 atcgattgca tcatgttgtt ctggaacttt agattctgga gttcctggat gatgaggtgc
     3001 atgcttttct gcatcatgtt tcttctcttc tactccttgt tgaacgttag ggactactgg
     3061 atctggatgt gcagatactg gtgctgtttt gttcttttca tcgttcacac catttgattg
     3121 gtttggtgtc gttgggtctg gatgtgcaga cactggtttt tggttgttca ttttattgtt
     3181 attatttttg ttattgttat tcatggatgt ttcctcctaa gatttaaaat agtgatctaa
     3241 gattttcgtt tgtttcagat aaaaaccgat agcgacgccg atcgctgcca tgatcaagat
     3301 caataaggtc ttgaaaaagc ccacggtgat ccataaaacg gctaatatca atccaagtac
     3361 cccaaagatg atagggagct tgtatgtcga aaatagttct ttcatttttg gtccccccct
     3421 attctactcg tttgcggtca ttgcttcgtt tagctggttc ttcaaagcct gtgtatttca
     3481 ccgatacttt cactggctct tttgttccca atatgtgttg tacttcattt tcgatttctt
     3541 tcatcaatgt tcctgtttta ccaatgagtg ctgaagttcg tttcaactcc ccttttacat
     3601 tgacggcgat cttgtttttc gttgctttga catttacttt tggtgaatgg atgaattcat
     3661 cttcatgcaa gtgagaacga accattcctt caattgcttt tttatccaac gtcaactccc
     3721 catcaccatg tttcagtaga aagtttccga ttgtttttgg ataaaacagg aaaacaagta
     3781 acgtgataat tgtcaaaata gcaaatgcaa cggcaagcca aaatacatat tgttgcacgt
     3841 atgaattagt gatggaaaag aatcgaaact tctctagctg aaaaggcaat ggataggttg
     3901 cacttaatag tatcgcaaac aaaagcacag atagcaaaag taatgttccg atcactccga
     3961 tcgtttttac acctttattc attttcgcac ctcctcatag gggtgaaacg atcaacttct
     4021 tataagttga tcgcttctcc accagttact ccgtaaattt gggctgtaac ataacttgct
     4081 ttattcgatg ctaagaaaac ataaactggt gctaattctc ctggctgacc tgcacgttct
     4141 agtaaacttt gctgaccaaa ttcagggatc gaatcagaag gttgtccttc gtctagttgt
     4201 aatggtgtcc agattggacc aggtgctaca ccatttacac ggattccttt aggtgcaagc
     4261 tgttttgcta aaccgatcgt aaagttagcg attgaagctt ttgttgctgc gtaatctaat
     4321 aaatgttcgc ttggattgaa tgcttgaaca gaagttgttg ttacgattgc tcctcctgct
     4381 gggatatgcg gaacagcttc tttcactaaa gcaaacatag cgatgatgtt tatcataaat
     4441 gtgtctttga cttgctcaag tggtaactca gcaatagact gacaagaaat ttgctgtcct
     4501 gcgtttagta ccatcgtatc tagcccgcca agttcactca cggctttatc tatgatttct
     4561 ttaggtgctt gttcgtcacg aagatcaaaa ggtagtaaga caatcttacg tcctgcatct
     4621 ttcacgtatt ttgctacttc ctctgcatct ttttcttctc ctgggaagaa ttgaagagcg
     4681 atatctgccc cttctctagc aaatgcaatc gctgctgcac gaccgatccc tgaatctcca
     4741 ccagtgatta gtacacggcg gttttctagt tgattattgc cttcatagga ttcttctcca
     4801 caatcaggaa caggaatcat attctgctgt aaagctggtg tatcctgatc ttgtttagga
     4861 aatttctccg tatggtacaa gtttcgcggg tcgattaatt ttggttctgt cattgctgta
     4921 ttcctcctag tttttaaatt gtattgccaa aacaaatttc tttgtttgat acctgtttta
     4981 aatagattct ttaatttgta taatctttcc ggtataaatg gtaacctaaa actactgctg
     5041 ctatcaaaga gccaaagact acgagtataa agcctagagc agaactttta cccggtaatc
     5101 ctcctgtatt cattccccaa atccctgcga acaacgccgg aacggaaatg attaatgcag
     5161 ctgaatctag atatttcatg atatgattta gattattgtc catcatcccg ttgaatagat
     5221 caccaatact ctctaataaa tctcgataga tctcgatcat tttttctgtt tgtcgctgtc
     5281 tcaaatggac atcatagagt agctgttcat cgtctaaacg tttgatgaaa gtttcagaat
     5341 tccataattg atccattgtt tcccgttgat ctcttaaggt atgatcgata tatacgatgt
     5401 ctctttcaag atctgcctgt ttatacagtt cttcattttc cgttgtttta cgagctgctt
     5461 tatccaaagc gtcaattcgc tttttcatct ctcttagttc tttcacgtaa tgggtgtaaa
     5521 tatctaataa tgtatacgtg atgatctttt caaacgagtt taaatgattt ccatgctttg
     5581 tcagcagccg atccacaaat tcggtttttc tatcgataca agtaatcaag agattatcgg
     5641 aaagtacaaa agatattggc gttaattttt tttcgatacg attatctgac tgatctagat
     5701 taatcactac caaagaaaat ggatgctcta gtttcgtctc ataaaggcgc tccatccgag
     5761 aaacttcttc cggatattcg gttcctacga aaatatcatc aggaagctcg aattcttcga
     5821 ttgcttgctc aatctcttcg ggctttgcac tatcaagtac gagccaatta tatttttttt
     5881 catctgtttc tattacattc ttttcttcat ccacaaagta gtatctcaat gtatacctcc
     5941 tttttggact ttctcgaaac tattagctga agaagcagtt ctttaatttg atattataat
     6001 cgagtactgc gatttcattt tctaatgaaa agctgtcaag ctgtttgttg aagtccatgt
     6061 tcaatcgttt accgaaataa ttttgctcta atttagatgt atagatgtcc aataatggat
     6121 gctcgatttt agtcgcatat tctccaaatt ccgaaacaaa aggaatgatt tgaaaaaagc
     6181 tgccttttgc aaaagcacct ttgtccctta gaaatggatc ggaatcattg ataagcgcga
     6241 gtgcgatttt tgtattttcc ccaacctttt caattaacgt ctctactgca tttttagaca
     6301 ttgtaatttc cattcctact ccccctcttc agttgttgtc ttctattatt taacaactac
     6361 atctgttttt ggttgaactt ttgcatattt tggatattta gggaatttag ctacacaacg
     6421 gaaagccgtc atccctgcat atttttcgta atatttcttc gttggccctt caaaatcctt
     6481 gctccacggt ttcacatgtc ctgaaaaatg gataatgtaa ggattgttgc gagtttcttc
     6541 gtattcacgt tcacctgctg ctgttggatg tttttttgct tttgccatga tatagccttg
     6601 tgcattccaa cgcggatgaa gtggtaacca acggtcatgt aagatagcat ttaatgcatc
     6661 ttggtcatgg aatcgtaatt tttctggatt atgttcgatg aaatctaata ctttttgcgt
     6721 aatattttca tctaaccatt ttttcacatt gatcagcatc aaacctgagt tgaaataacg
     6781 cattgattta gctgaaattt tcattttttc cagtcgttga tggaacccag cgtcttcaac
     6841 agctgctacg attgaatcac cgaagtcaag acgccataat ttagagatat cttgtaaagc
     6901 gatcatgtcg caatccatat ataagatacg ttcaacttct gtgccgcgga acaattccgg
     6961 aatcgcaata cgataataag ctgtttctgg aatacgatcg ctgattacca catttgtaaa
     7021 gaattcttta ttgattttca agaattccac atctgagttc attcgtgcgt ttttcactga
     7081 gaagcgtaaa agctgtttac tttcaaaatc gatatcatcg tcaatcacat agaaccgaac
     7141 aaatttagac gggttgcaat tatctaatac tgagacaaat aatgcagcta aatgtggtac
     7201 gaattttgtg ttacaacttg cgactactgc tatttctttt ttattcattg atctcgccta
     7261 ctttcgttag agtactgtgt gctaattttt ttaagtataa attggtatac ggatgatctt
     7321 tcaacgtatt ccaaggttta tcatgtcctg taaagtgaac gatcgaaggt ttttcatttc
     7381 cttctttata ctgacgttca tatttttcat ttggtgctgg atgacgttca aaaatcagcg
     7441 aagtctgcat gttccatttt ggatgaagtt gttcccaatc ttcataaagt acagcattca
     7501 aagcatcttg gtcgtgatag atgattcgat cgccattttc actgaggtaa tggatagttt
     7561 tttctgtaat ttctttttta ttccattgat caatgtcaat caccatcact cctgaattga
     7621 agtacaagtc atcggaatcg attcctaatc tttccaaagc tttcacttgg cctggatcga
     7681 taatagcgcc gattgttttt ccatttaacg gctcatcata tagttttacg atatcgtcta
     7741 gtactaacac atctgaatct aagtaaagga ctttcttata atcctcttta gctaataagt
     7801 ttggtaaaga aattcttaag taagcggtag ttgtgatatg gtcactgact aggaagtctt
     7861 cgtagatatc tttttccact gtcaggaatt ggatacttgc attacttgaa tatttattta
     7921 ccgtttcttc caatccttct ttactatatt ctgatagacc atcgtcaatc acataaaatt
     7981 ttatgcgtct tgttttattg caattttcta atgcagttgc gatcatcacg ctcaagtaag
     8041 gagcataatt ttcgtctgaa gcagttacca caggaactac gccgtatctc ttctccatca
     8101 aaatcactcc tttttctaat ctttttctaa cctgttctac atctagaata ttcctcattt
     8161 ggttttaaag caaaatatat gctttgtttt cattgaatat tttaaaattt ttttatcaca
     8221 aaacgcatcg gtaagcgcta actattcgtt tttcatggct ttttattcaa aggtat
//
LOCUS       DARDHU010000105         8235 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000105 DARDHU010000000
VERSION     DARDHU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.106"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..935)
                     /locus_tag="SDD03_002391"
     CDS             complement(<1..935)
                     /locus_tag="SDD03_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG4304816.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKE"
     gene            complement(1009..2088)
                     /gene="uxuA"
                     /locus_tag="SDD03_002392"
     CDS             complement(1009..2088)
                     /gene="uxuA"
                     /locus_tag="SDD03_002392"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4304817.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(2091..3722)
                     /locus_tag="SDD03_002393"
     CDS             complement(2091..3722)
                     /locus_tag="SDD03_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG4304818.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            4039..4710
                     /locus_tag="SDD03_002394"
     CDS             4039..4710
                     /locus_tag="SDD03_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4304819.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(5546..6502)
                     /locus_tag="SDD03_002395"
     CDS             complement(5546..6502)
                     /locus_tag="SDD03_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304820.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(6611..6856)
                     /locus_tag="SDD03_002396"
                     /pseudo
     CDS             complement(6611..6856)
                     /locus_tag="SDD03_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7001..7423)
                     /locus_tag="SDD03_002397"
     CDS             complement(7001..7423)
                     /locus_tag="SDD03_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304821.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(7603..7974)
                     /locus_tag="SDD03_002398"
     CDS             complement(7603..7974)
                     /locus_tag="SDD03_002398"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4304822.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         2584 a         1666 c         1231 g         2754 t
ORIGIN      
        1 tgttccttta atatacttga attatttctt aaagcattta aatgcatttg catcgtccaa
       61 ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt
      121 tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt gtcgagtatt
      181 ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa tcgacagcca
      241 atttgatgaa aataatccac tcgttggctc aatgcctgct tcaacccatt aaagtttttg
      301 attggtatac ccgctgcttg actaagtttt aaaatatagt tcgaaaatcc tgatgatttg
      361 atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt atcctgttta
      421 gaaagctcag tatgatatcg caagtcatct gtaggatcat ccgttgtaca aatggctttc
      481 acatccatca tcttcagcaa ttcttttggg cgataggaat ccgtttggat tttttcgttg
      541 gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa
      601 cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt ctccactgtt
      661 tttacaaaag ctaaaaattt ttcataatca ttacctgcac cactgatcaa ctcttcttct
      721 atcccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt atcatttaac
      781 catacctgag tcaaattctt gtattgctta ttttctagca aacatgctgg gtctaaatga
      841 caatgataat cgatgattgg ttggtgttct gcaaatttat gaaaaagtct tttagctatt
      901 tcattctcta ataaaaagtt cttatctaaa aacatattta agcactcctt tccttccctt
      961 agtttttaga agagaaaatt tttcttctaa atagactcca ttatataatt atttactcgc
     1021 ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag ctcggtcata
     1081 taacccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat ctggacgaat
     1141 atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat ctagagaacc
     1201 atattctgat ttatgcggcg cttcataaaa atcgagtcca ttatcactta aatatttgat
     1261 attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata gaatttcata
     1321 gacatcatta tctggtcgtt ctgcaatact tccagtacat aacgtaatac cattagaagg
     1381 agaatccact acattacaca accattctaa atcgtcacga tttttaacaa tacgaggtaa
     1441 tccgaaaata gaatatggtg gatcatctgg atgaactgcc attttaatcc caacttcttc
     1501 acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgcaatt tcgcttcatc
     1561 aacttcttta taggcttcta ataattgctt gatttgtgat aaccgatgag gttcccaacc
     1621 cggcaattca aaattattag tagaattttc tacttttcta ataatttcct ctggatcagt
     1681 aggaatattt tttgaaatat aggctaacgt agaagaccca tcatctaaat gataatccaa
     1741 atctgatttt acccaatcaa agactggcat aaaattataa cagataacct caatcccaaa
     1801 ttctgccaaa ttgcgaatcg tctgtttata attctcgatg tattgatctc gtgttggcaa
     1861 accaattttg atatcatcat gaacattaac actctcaata actttcaagg taaggccatt
     1921 ggcttcgact tcatctttta aagctttaat tcgttctctc ggccagacct ctccggctgg
     1981 tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa tttcttgtag
     2041 tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag ttactttcct
     2101 ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc tactagcatt
     2161 tcgcaaaaga ttttttcaat tttctttcct atctcaactt gatataaatc taaaccaaat
     2221 atttccttat tttctaagat ttcctgtacc gcacaatgaa tctttgcagg atcttgttca
     2281 cctaatttca atactgagaa tttttcctgc aactctacca ataaaggatc aggacttggc
     2341 acaaaatttt ttccttgatc atcgactgcc aataaataac gtaaccaagc agcaatagcg
     2401 ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt tttaattgtt
     2461 tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg cggtgtatct
     2521 ggaatatagg gattaggcaa acgtttattg attacttcat caataaattg ttttggatca
     2581 ataacgcctg gactttcaac tactggtaag ccttctacat aacctatccc tttaattaag
     2641 ccaaccaaat catcatcttg catttctgcc tgaattgatt tataccccaa caaacaacct
     2701 gttattgcta atgccgtatg gagcggattt aaacatgtcg taaccttcat gatatctgct
     2761 ttatcaactg tttctctatc tgtcataatc acaccagccg cttccagttt aggtcgacca
     2821 tttggaaaac tatcttcaat gactagataa tggacttttt cagtattggt aaaagcagcg
     2881 atattggtat ttttcgcagt atgaattata tctaagtctt cgaaacccgc cttcaataaa
     2941 tccattttga cactttccga aggatttggc gtaattctat caatcatact ccaaggaaaa
     3001 gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacgta tccttttttt
     3061 tgccaagctt ctgcaatcat aaaaatgcta tcttgaaaac gttgaccatt ctgtgagaaa
     3121 ttatcagtag aaaccatcgc aatcggtaac tctccagcat taaacctgct atacaacaag
     3181 gatgtcacaa tactcatcgt atgttgagga ttggtaggtc cagattttat atctgcttcc
     3241 actaccgaaa aaaattgtcc tttagaatct tttaaactgt atcctttttc agtaatagta
     3301 aacgttgtaa actgtaatgt aggatcttca aaatatttaa ccactctttg atagtccgtt
     3361 atattctttg gattgcaata taaactatca gctgttactg ctaaaagtct ttttttgagt
     3421 tcaccatctt cacccataac aatctccaaa atatcattgt tgtagtcatg ataagctttt
     3481 tctactactt cttcatcaaa tgtctcacac acaactactc cggattctaa ttcattttga
     3541 tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc tccaccaaaa
     3601 tgaagccaca ctggatgttt ctgaccttct tgtttgaccg cttcgacatt aaaatacggt
     3661 agttgaactt gcattttgtt caatccttcg atagaatctt gccagtcttt aatcaaaacc
     3721 atatttaccc ctccatactg atatgttagt ttccgttaca attgtagtat aaaatcgaat
     3781 gtaatcgctg tcaataagaa aaaactaaaa atattctttt ttctaccatg tcagaattat
     3841 tgtgtctttt tttagtaaaa gcacaccaat atatattttt tagaactaac ttaaattaaa
     3901 gcttaatttc tctttgatct tatttgagta ttttttgttt ttttaatagc tagtatgtta
     3961 ctttgaaatt tgatattata taacgagcat agaactaaaa tatcaaattc aaaaatgaaa
     4021 ttttgagagg gattaaatat gacaattaaa actagtctcc aaaatcaagc ttatcaatct
     4081 attcgtcagc aaattatcta cgctgactta gaacctggaa aaaaagtatc agaaaaaaaa
     4141 ttggaagaaa cgctttctat cggtcgtact ccaatacgtg aagcattgat tcaactccgt
     4201 catcaaggac ttgttgatac aattcctcaa agtggaaatt atatctcatt aatcgactta
     4261 gatttagcaa aaaatgctcg ttttgtcaga gaacatttag aacgacaaat tatgctagag
     4321 tgttgtgcca agatgaataa tcaaaagaaa caagttttag aaactataat tgcagaacaa
     4381 gaaaaagctg ttagtcaaaa gaatgctcgt agtttttttc ataatgataa tttatttcat
     4441 aaagcttgtt ttgaaattgc agaacgggaa gaagtatggc aatggttgga agataataat
     4501 acgcacttgg aacgatttcg ttggttacga gtaaaaacgg aaggattaaa atgggaaaaa
     4561 attatggaac aacatcatca actatttaaa gccttgacca tgcaagaccc tgaagaagct
     4621 aacttcttaa catccttgca tcttcattta atgcttgatg aacaaaatgt tgtattgact
     4681 actcacccaa aatattttaa aaatatataa ctgctaaatt tctttttttt aatttattta
     4741 catttctgat tactacagaa aactaactac tttttttatg taaagttctg tcaaatatag
     4801 tcactttata ttactttctc ttgagtaaaa aaacatgtga ggtcgtaaac aaagattgaa
     4861 ctatctacat tcatactcta taatctgttt atttgcagac tgataatagc aaaagaaaac
     4921 ttataatcaa ataatatcaa catttgatca cataatttga gattgaatgc tgatttacct
     4981 atgttttacg taatggtctt tagttatttt tgctcataaa atgcataaaa cacgcgtttt
     5041 tatgtgtgct tgaaatttct tggctctata atttctaaga ctgataaaaa tattctatcg
     5101 gttctttttg caaataaaaa aacatataga agctccatta gattgaatca ccacaacaca
     5161 atcaaaggag cattccgtat gcttgatttt cattctataa aactactttt aggaatcaaa
     5221 gaaaatcaca acggttcaaa ctaaaagcaa gaatatccga agtaccattg tttcatcgat
     5281 catgagaaaa gcattcacct ctgtctgcag atgttcttat atcgctctgt cagtcctatt
     5341 aataaagtac tgttttgttt tcaaaaaaac tattacgagt actttttaag agaatgcctt
     5401 gcaaatattc atcacacacc gttatataag tcaaatagtt tctttcagtc gtcaagaaaa
     5461 gattctcctg tccttggcat tatcagttaa gtagcaactt aattctcgga cttgttctac
     5521 tctagcaaaa tttattttca tcaacttacg tccaaatgtt tttttgaata attacttcat
     5581 cgcgggtctg gaagacttgc ctcattcgat aagtcactag cttttggcgt ccttcaggat
     5641 ttctagtata caccaactga catttgggca gcgacgattg atttaattcg tttagaaaaa
     5701 ggttagcgat ttgcttattt cggccaaagc gttctggtac cgtcagaact atctttttcc
     5761 cttcgattaa ataccgcgga ttctccagcg gtgctaaaag ctccctcata gcttcagtaa
     5821 aaagctgtcc ttctcgatag ccagcattct caagatagca tctaaattcc tgctgatcag
     5881 ccgctacgat tacttttcta tcactaactt caccaaggcg ctgcaacgtc gtttgaacgc
     5941 atatggccaa gctttttttg cgggcacatt ccttttccca tagcttttgt cgcctacgaa
     6001 tttggaaata ttccagcaag ttacctttaa atatagctga ccagaagctt agaggctcta
     6061 tattttttac tactatcggc aatgaagttt cttgtttttg ctttacctga tcgactttcc
     6121 taacgacgat tggcatagat aggtgccagc catcagctaa agcagtactc cattgttctt
     6181 tcaaccgact tcgttcacaa gaaaaataga acatcgactg agtataagtt tccatcgtgg
     6241 tggtcgtata ttccagtggt aaagcaaagc gatcgccacc actctcaatt tttggtgaag
     6301 attcaaaggt cagacctgaa atattgccca aacgccgtag cattcttttc aaatcaggct
     6361 gttcagacag aggaactccc ggtaatccaa tcgctaaatg ccaaatgttc gttactttat
     6421 tttgtgcacc taggcgtaag ccccgccctc gtagttgttg tgtttgcaca aaagtggaag
     6481 cttgattagc caagatcaac agattaattg caggacaatc ccagccttct cctagcaaac
     6541 taacggttcc taccaaaata ttcaacttct tttcggttaa taggcgagta actgcttgca
     6601 tcaatgcttg gcggtttttg tcatccaccg tcaaataaaa ataacccgac atataaggat
     6661 gttcattcat acccgtcgct agttctgcca gctcaacagt tactagcatt ttcggtaaca
     6721 gtaagaactc tccacagatg gcaccgattt gctgagtgtt acctaataga cgaacaactt
     6781 catcaaacaa aggaaccacg ccataagcta aggtaccatc actgcctgct aatacctcct
     6841 tgcaaatacg atccatcaaa atgaccgctg tcagttcttg cttcagctga acttcttctt
     6901 ttaacaaaat ttgacaaact gcttgctttt tctcaggaat cgcctgacta ccaacagtgt
     6961 ctggatactc tggaaacaaa tgcagcgact catcaaacaa tcatccagct tttttcaatt
     7021 gataaaaaat aggcaattcc ggttgatctt caaacatctg ctgataaata gtgatcagct
     7081 cttttttacc taaagatgga actgaagctt ttttaccttg ctgcttagaa ctgcctaata
     7141 gctgccaatg ttcgggtatc aattcatagt tatgatgatt caagtaaatt aatgcactag
     7201 tgaacagctc aaaattttca taaatttcct tcgaatgctc aagcggatgc tgaacaaatt
     7261 ctaaagaaag caaataatcc accgtctctt ggctttccat taacgcttcc accagctcat
     7321 tttgtttctc taaaaaggta aaataatgct gagtagtttc ctgattagcc ggaatcaaat
     7381 atagaaaatc ctgatgagga catagaacct cctcctttac caactcggga atgctaattt
     7441 cttcgtcaat caggccattt taactcaata tattgcttcc actccgtctg agagctatca
     7501 atcggaggcg tggctgtcaa ggaaatcgtc aacagattcg gttgtttttt cttcaagtca
     7561 atcaacaatt caccccagct tttttttaaa tgatgagctt catcaaaaaa ctaacagctc
     7621 cagctgatca ccataagttg gctcctcctt cagtcggttg taaatggtct gatacgtttc
     7681 cactgtaata tgactaggat gttcaatcgc cccagaaaga tagcaactat ctgctggatt
     7741 tactaagaaa tattcctcga ttgttgttaa ccactgtttc ttcaatatta gggaaggaac
     7801 caaaattaag gttttacaat ccaaacgtcg gatcatctca attcctaaaa aggtcttgcc
     7861 agcccctggt ggagccacta gatggactga attgttattt aaataagtag caaagttagt
     7921 caatatacgc ttctgatagc tacgccactg aattttttca tcagatagcc acatcccagg
     7981 tccccctctc acgtattttc tctatcataa tctaaatgaa tacagcaaaa aaaagatttc
     8041 ttcaagaata tgtgttttta tagtaatatt ttacttactc taaactcaaa tgaaaaggta
     8101 aaaaaactgg tagtacaatc ttttaaattc tcattgtttt gtacgttctt tactatcaaa
     8161 aaaacaaaaa aaacgcgttt ctacgcgtga ccaaaatttt tagctctata atttctaaga
     8221 ctgatagaaa atcat
//
LOCUS       DARDHU010000106         8164 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000106 DARDHU010000000
VERSION     DARDHU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.107"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(116..1751)
                     /locus_tag="SDD03_002399"
                     /pseudo
     CDS             complement(116..1751)
                     /locus_tag="SDD03_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2306..2869)
                     /locus_tag="SDD03_002400"
     CDS             complement(2306..2869)
                     /locus_tag="SDD03_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4304823.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2884..3927)
                     /locus_tag="SDD03_002401"
     CDS             complement(2884..3927)
                     /locus_tag="SDD03_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304824.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(4060..4464)
                     /locus_tag="SDD03_002402"
     CDS             complement(4060..4464)
                     /locus_tag="SDD03_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4304825.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4697..5332)
                     /locus_tag="SDD03_002403"
     CDS             complement(4697..5332)
                     /locus_tag="SDD03_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG4304826.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5598..6650)
                     /locus_tag="SDD03_002404"
     CDS             complement(5598..6650)
                     /locus_tag="SDD03_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4304827.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6663..7601)
                     /locus_tag="SDD03_002405"
     CDS             complement(6663..7601)
                     /locus_tag="SDD03_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4304828.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         2638 a         1636 c         1290 g         2600 t
ORIGIN      
        1 tagcccaatt ctctataaag aagattaagc ttcattatta taatataaag tttaaatttg
       61 gtttattact tactttttct tattctctag taagctaata ttgaaaaaaa tgtaatcaga
      121 tcattatttt tatatgttgt aacttcctga taagaaaact aattaaaaga aagaggtaac
      181 tacataaact aattaaaaaa gctaacttca tagttggagg ataaatggaa gaaacttcaa
      241 tgattgtttc tccttgtgtt ccttcaatcg acactagtcc tattatcaat ctctcttttt
      301 tgtccattta cttttgcgac tgttccccaa taatcaaata caggaagcgt tattttttgt
      361 cctttagatt catgtactgt atagactatt ttcgaatcca tcacttgata aggcatagta
      421 tgccattcct tgccaattct tacttcatga ttctgcaaat gttctttgta ttgtaatgct
      481 tccgtgggtg catagtcata aagtcctccg ggaaggagtg tcgtgtaacg aataagattt
      541 tcttgattca ttaccaagtg atctctctga gtgacaactt tatgttcata gtttgctttt
      601 gttgcagcag atacagccaa cagtaataaa acaattgccg ttatattaac tacttttctt
      661 tgcttagctg atgtgctctt catgaaaacg aacgataaaa tcaacgagcc aaacagtact
      721 tgaaagccta agattcgcca aggaaattga atcatttgaa tgggtgtatt ttgtaataaa
      781 gaccatggaa aaaatttggt ggataagagc caagagacta ccgcactaag gtaaatcact
      841 ttttctttgc gattaaatcc tttaaaaaag aagagtggac agatcaaaga aataatacca
      901 atgattccta ccgtaaaggt tgtcaggtca ttattcatcg caccaatcaa actatccgag
      961 acatttaatg cttggtgttg taaggtcgtt ttatctggct cattaatcga ttgaaccatg
     1021 atttgtcgga acatcggata ccaaaagtat gctgttaaaa gaatcgtcat tcctactgcc
     1081 tttgacaact ccacaatcaa ccggatgctt tctttttttc ttgtttcact tactacaaag
     1141 ccgcctaaga ctagtaagcc aacggctatt acactcataa aagtggaaag aacatgtgta
     1201 taaatgagta agctcatact gactgctaat ggaattgcac atggcttttt ctcataaatc
     1261 agtgcgtaag cataatacat aactatcggt ataaccgtaa gtgcaatcgc ttcagcaata
     1321 gcagaacgat gataaatatc aatcgtacgg tacccataaa atgtatataa acagctaaat
     1381 ataaaagccg catcgattct ttttaaaaat tttagtccat aatgaaacat cagcatgata
     1441 gtacaaacat tcaatagcca aacatataag atataactaa cgattaaatt attacttatc
     1501 caataaaaaa tgactgccgg aaaaaaggtc aagtagggat aaaaataatt cactccgctc
     1561 ccataatgat taaacgtcgt aaaattgatt ggaccagaaa aaattgaaga taaccctttg
     1621 actctagcaa aatggaaggc aatatccccc gatgtagaaa tagtatgaac atttctaaaa
     1681 taattgattg aataaagtcc agcaataatt gctaggagaa tggacaatat tatttttttc
     1741 tttacttgca ttacttaatt atatactcct ttcatggatt gctttatcta gtataactta
     1801 aattttaaat aaatcgttat ttatttttac tctcacttaa aaaagcacta aaattataag
     1861 agagggaggt aatggtctga acaccaagaa ttgttccaac atcgaattgc ttaactaatg
     1921 agttcaaagc ttaattatgt ggctcttata tttaccgagc gtggattgcg atctctttag
     1981 cgaaacaaac atctttcata aaaaaaacag ccatatccgc tttcttggat atgactattt
     2041 actactactt tactatcttt cattcttctt gtctcactta tgataaggcg ctgcgtatca
     2101 tgagtgaatg atattaggaa aacttcataa aataaatctt ttatacattt ccacttctag
     2161 atggtcattc atgttagaag aatcaaacta tttttttact gtcgtaataa tattaaaaca
     2221 ctagaacaaa tcaattaaat tccataaaat ataaataata gacctagcat catctggtat
     2281 aaaatggatg ctaggtccgt tatatttact caaaattgat tctacgaata gatcttctta
     2341 tcatttctat tttagtagtc atgcgttgtt gttcttctaa aggtggtaat ggtataagta
     2401 gtttagataa atttgtacca tttacatttg gttgcccagt tccgtaagaa attttttcta
     2461 ataacttcca atataatggc gagtctaaga agcagtctac gtattctgat aataatttct
     2521 caactaacct aattcgaatc aagtaagaag caaaaacaga ttcctcactt atttccttaa
     2581 ccaagaaact ttttcccata gttcctcctg tccttgctat caaaatgtca ttttcttcca
     2641 atcttaaatc aactagttta gaattactaa tatcacagta aggcacactg gaccaattta
     2701 cacgtccctc ttgtatatct gtgattctca aaaacttaac attgccttgt tttttcgccg
     2761 aatctgtata gccatattga atcttagtag aaatacttcc taaataaacc catagtcgaa
     2821 catcgctaga tgatttttct tgtcgttctt tatgaacaac agtgatcatc ttgagttgtc
     2881 cgactatttt ttagttgcag caatcttttt gggacgctta tttgcatgaa gcaacgttgc
     2941 taagtctagt aataaaacat tggctaacac aggattggct ttctcgcgat acgctgcggc
     3001 gccactttca aacaatactg ctagacgatc ttctacaaca acgatttgtt ccaaatacgg
     3061 tggaaatttg attcgtttta ggatccggct aggagagtcc cgctcggaca ctaagagttc
     3121 cgaggtataa ggaccataag aacgactggc taagagataa tggccataaa aagccacacc
     3181 ttgaatctta tcagatgttt ttctgctcat tgtcacggtt ggttggattt tgtctttgcg
     3241 gcgaattgct gttttcattt cagctatcgt cggcaataga tagaaagtca actgtccttc
     3301 accttttagt gtgaagttgc cagataccag ttgatcattg ttttgagtca aatacgatgc
     3361 ttgtggaatc tctgagagat cagttgtcag ccaataagcg attggctgtc ccgtctcttc
     3421 agatgtatct gctaagatat cgatcaaacg taaagcggct actcgtccag tagcagatcc
     3481 tgtgattgtc aaccataaaa gttcccgttt tgtatcatat actaagcccc ctacatgtgg
     3541 ccgtttaggt aaaatcagca gtttgatcgg ctgtcctgtt tctctatcca gtacatgaat
     3601 aatagaatga tgctgatggt catagcagta ggcagaaata aacacaaaat cttgagaaaa
     3661 agccaaccct tgcggcgtca tccattgaca aacctcattt tcgccggttt ccggcaccac
     3721 cattcttgtt tctgttaatc caggaatgtt atattgtttt gcttcagcct tttcccaagc
     3781 tgcttgaaca ataggatacc gttttaactt tttttcaata tcctgatgta tccgttgctg
     3841 cttccaatgc ttaaacttta ggacatagta aacaagaagg ccaatgacca gcaacactaa
     3901 aatcattatt gctacccgct gtgtcatttt tttgcctcct tgttctattc gatcaatcgt
     3961 ccatataccg ctattaatta ccgtaactac atgtttcttt tactagcgtt tctttatagg
     4021 atctttcata atctcattta tagtaacatt aagaatccct tattctacta tagtcacttc
     4081 cacatcaata ttgcctcttg ttgctttaga gtatgggcaa aatgcgtggg cttcttctgc
     4141 gattttctct gcttggtcct tctcaagtcc tggcaaagaa acttctaaaa cgaccccgat
     4201 ttggtagccc ccatcttcta attgaaataa agatacccgt gctttgactt cagaatcaga
     4261 ctcaagatta ttttcttcca gcatgtgttg aaccgcgccg ttgaagcaag atgcgtatcc
     4321 agccgcaaaa agttgttccg gatttgtccc ttcactatgg attcctgggc ggtcgatctt
     4381 catgtgcata cttccgtttg gtgcctcaac ttcgccatca cggcctccac gattgatcat
     4441 tgctgtttcg tacatttttt tcattgagat catcctttct ttcgtcgttc tatattaagt
     4501 gtaacgagta ttttttaatc tgacaaaggt aatgcttgca ttcggcgaca tcagttagac
     4561 aacatcgatg atcagtgata gaagcagttt atctatacaa aaaaagaagg gttctatgat
     4621 atttggtgtc cttactcgcc ttgagtaaca acaccaaata tcatagaacc acttaatcag
     4681 cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc atcggcaaat
     4741 agttaaagaa aaaacttaat cgttgaaaga agcccaattg cttaaagaag gcaaactgac
     4801 taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta ataactgtta
     4861 aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc agaacaagta
     4921 acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta tggatccaag
     4981 tcgatagatt acaagagctt tcattcgtat tgatactgaa taagccagta aaaatacaat
     5041 cacctatccc atataaacca actgttgcaa caacaagtat tgcaagtgtt tttgatgttc
     5101 caacaaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca gatacaactg
     5161 accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca ctaattacgg
     5221 tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc gataaatcac
     5281 tcatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc attttcttcc
     5341 cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca ttttaacaca
     5401 tatttttatt ctagttgtct tttgaacaat caataacata aaggtgagtt tcttctacat
     5461 tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc tactcaaaaa
     5521 ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat tgattgaaaa
     5581 tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc atacagttta
     5641 aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat gactaaccaa
     5701 caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg accaacaagt
     5761 ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag agcgacagga
     5821 atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt tctaaataga
     5881 ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc tgacttatta
     5941 accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat cgcagccgca
     6001 ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga aataggagag
     6061 ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg ttgtaaggta
     6121 gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa agggaataaa
     6181 taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac aatcgacact
     6241 gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc tgtcagcatc
     6301 gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc caaatcaggt
     6361 gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat cattctcatc
     6421 ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt tgctagttga
     6481 aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa atattttaaa
     6541 atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc tgtaccagcg
     6601 aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat tcaatctctc
     6661 ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa gaaatcacaa
     6721 taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt gatataatcg
     6781 atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata atgtaaccca
     6841 attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac atctagttca
     6901 atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac cattttatga
     6961 gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca tagtccgttc
     7021 attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc agaaaaatga
     7081 gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc ttcttttcca
     7141 tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg accaggacct
     7201 tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg tacaaatttc
     7261 tcaataatat cttcatgccc gataccattt aatagacata agacctctgt atcttttttg
     7321 attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt aaatagaata
     7381 atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg tagttttgct
     7441 tctacctctt taccattaaa atttgcttgt aatccatgtt ctttgatttg ttggacatgt
     7501 tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat caacccaaag
     7561 cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc ctcctttttc
     7621 tttctattat actcatttga gaatcttttc acaacctcgt tttgttattc ttctcttatt
     7681 tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa aactggaata
     7741 gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga aacattcata
     7801 gaaaaaatct cactcctctg attataaaag agggggcatt aacctcgtgt atactaaaac
     7861 cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg tagtacgatg
     7921 ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca atacacttat
     7981 ttaaaaactt ctttcccaga ttttcttcca tttttacagt tataactagt attatcatga
     8041 aaaatagcct tttattgtct atgggaataa taatttaaaa ttggtacttt tttattaatt
     8101 agttcatcgc ccaataatct ctccagtttc ttagcatctc ctgactcaac cggaattatc
     8161 caag
//
LOCUS       DARDHU010000107         8162 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000107 DARDHU010000000
VERSION     DARDHU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.108"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..903
                     /locus_tag="SDD03_002406"
     CDS             202..903
                     /locus_tag="SDD03_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4304829.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1512..2528)
                     /gene="tsaD"
                     /locus_tag="SDD03_002407"
     CDS             complement(1512..2528)
                     /gene="tsaD"
                     /locus_tag="SDD03_002407"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG4304830.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2525..2989)
                     /gene="rimI"
                     /locus_tag="SDD03_002408"
     CDS             complement(2525..2989)
                     /gene="rimI"
                     /locus_tag="SDD03_002408"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG4304831.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2996..3538)
                     /gene="rimI"
                     /locus_tag="SDD03_002409"
     CDS             complement(2996..3538)
                     /gene="rimI"
                     /locus_tag="SDD03_002409"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG4304832.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3522..4244)
                     /gene="tsaB"
                     /locus_tag="SDD03_002410"
     CDS             complement(3522..4244)
                     /gene="tsaB"
                     /locus_tag="SDD03_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG4304833.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4435..5415)
                     /locus_tag="SDD03_002411"
     CDS             complement(4435..5415)
                     /locus_tag="SDD03_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4304834.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5439..6923)
                     /gene="galT"
                     /locus_tag="SDD03_002412"
     CDS             complement(5439..6923)
                     /gene="galT"
                     /locus_tag="SDD03_002412"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG4304835.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6935..7924)
                     /gene="galE"
                     /locus_tag="SDD03_002413"
     CDS             complement(6935..7924)
                     /gene="galE"
                     /locus_tag="SDD03_002413"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG4304836.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2320 a         1729 c         1424 g         2689 t
ORIGIN      
        1 atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa ttattcaata
       61 ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa acttgtttct
      121 aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa
      181 caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc
      241 agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat
      301 actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa
      361 cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact
      421 tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga
      481 gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct
      541 gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa
      601 aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc
      661 gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca
      721 aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc
      781 ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc cgggctgtta
      841 gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa
      901 taacgcgaac aaaaataagc agtgaccctg ataaatatca gggtcactgc ttattttcct
      961 ccctaaaact tctgaaagat ccggcagttt tagatcactg gacaagactc aaagattttc
     1021 tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgtta gattaactag
     1081 atgaagcgtc ttgtcctcat ccgatctaga ttattcgata ttcctcgaca ataaacgatt
     1141 actcagtgag ggcaatcaga ggaacggata atgataaata agctgtaaaa aatagcaaaa
     1201 taacaataat gattaaaagt atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc
     1261 tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata atgttcataa
     1321 acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg acaggcttac
     1381 gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc
     1441 aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag ggcagctttt
     1501 tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg atagctagca
     1561 aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc attatctcca
     1621 caaagtcgaa gagggggaat cactagttct acctcaggga gtttagctgc caaagcatcc
     1681 tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctataat cagttgcttg
     1741 actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat cacacttgct
     1801 tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg tgcattatgc
     1861 accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt atcttcatgg
     1921 atcatcgctc gcggaaaatg gtaattatct tttccctgat gagctagctg atcgatttct
     1981 tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc ttctcctgct
     2041 gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat atagactaat
     2101 tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg tttaaccaga
     2161 cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc
     2221 caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag tcctggtcca
     2281 taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac cagtgcatct
     2341 tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg tactacgcca
     2401 ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat ctctgttcca
     2461 tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag aatcaattct
     2521 tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca tcttccgtcg
     2581 gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat tcataaagtt
     2641 ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa tcttttattt
     2701 gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg
     2761 caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca ataaagccac
     2821 agatctggtt atcttcagct aaaagcagat aatcagagtg aggctgttgg atgtctgcca
     2881 aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta atcatccata
     2941 cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac taatctcact
     3001 cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg taatatcttt
     3061 ttttgatcgt tgttgcttga aaccccaatt tccgataaag tctttgtgca tcttgattac
     3121 tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag ttctcgattt
     3181 cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg gcaatattcg
     3241 tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt cttccattat
     3301 caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata aaggcacttt
     3361 ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc tctttgatat
     3421 cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgact gttttttctg
     3481 tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt ttcaacataa
     3541 ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac tcgttttaaa
     3601 tagcgaggaa gaaaagcttg tttctctgca actggttcaa ctaaagcacc taattgagcc
     3661 acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc actaggcaat
     3721 atttccttta tttcagagaa gaatttgtga caatcactcc cgacgaaata aatagcttca
     3781 ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca
     3841 gtttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt tcttcgagca
     3901 tcaaacatgg gcacaataat attatccaca ccgatgcaat tagccgcgat ggttttcaga
     3961 ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc tgtcgttaca
     4021 cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct
     4081 tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag tgttacactg
     4141 tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc taccacaccg
     4201 atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt gatcctttct
     4261 gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat gaaaatacca
     4321 aaggcaaaac tagtacaata atacatgatt atctgctggc gggcaaattt tgccccttcc
     4381 tgaaacacaa aaaagctgcg gtatcactcc attttcccac agcttttcca tcttttattt
     4441 cgttgattct cgaataatca actctgtttc cacaataatc tttcttgaaa ccggtgcttt
     4501 ggaattactt aattgaagaa tcgtctccac tgctagctct cccatccatt cggtataaac
     4561 ttttacagta gaaagcgagg gcgttacata tttcgctaca ctgacatcat tgaagcccat
     4621 cacagaaata tcttctggta ccactaaacc tttttcttga atcgctttca gtgcgccaat
     4681 cgctaatgca tcattagctg caaaaagcgc atcaggaaca tgcggattcg tttttaaata
     4741 ttgcttcact gcttgatatc ctgactcgac agaaaaatct gcttcgataa ttggaccttc
     4801 gtcgtagcct ttttctgcca aaacttgttg aaatgccata attctgggat cttttagccg
     4861 ctgatggttt ttcttcgtat attctgttcc agcaagcata gcgatatgtt gcgctttatg
     4921 ggagacaaag tgttcaatga ctttttgaac acttgactta aagtcgacaa ctagagagtc
     4981 gattccttga gaagtgccgt cagaatctac caaaagcaag gcattttggg atcctttcat
     5041 ttctaataac tgagcttcgt cgaattttcc tagtgcgatc gttccatctg ccggaagatc
     5101 actaatggaa tcccacgttt ctttcaataa agtaacgttt gcttcttctg cctttttttc
     5161 gattcctagg cgaatagata aataataaag atcttcaagc tcttccgtat cgtcgtacca
     5221 ctggatcagg cgaagcgttt gcttagtatt tgctgcattt ttacgatgtt tcgtataatt
     5281 taagcgttct gctgcttcaa aaattttttg ctttgtggct aagccaacgg aaagatcagg
     5341 atcatagttg agaactcttg aaacagtagc aggagaaact cctgccaact gcgcaatgtc
     5401 tttaattgtt gccatttgtc tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt
     5461 tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt
     5521 gcaaaaacct gacctacaga tttttgtacg acatcggtga ccgtatcttt cgtcaaatca
     5581 ggatgttctt tttttaattg ttctgcccac ggacgatgat attcagccat ctggttttct
     5641 tctcctaata agaaatgctc tacttctact agttctggtt tcaaacgagg tggtaaaaca
     5701 gcaagcccca ttacttcgat caacccaatg ttctcctttt tgatatgttg gacatcttta
     5761 tgtggatgaa aaatcccatc aggatgttct tctgaaacat tgttgtctcg aaggacaagg
     5821 tccaactcaa agtaattgtc ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta
     5881 ccatctgctg aaaaagctct gatagatact gtttcatccg aatatgcttt ccatttttcc
     5941 aatactgagt tagctgcttc aataagcaag tttttctctg ttgattgcaa acggatcaca
     6001 gacatcggcc atttcacgac tcctgcgatg acttgcggat aatcttctag tttgataagt
     6061 ttttctatcg gtgcttttgc cataggaaat tcatgacgtc ctgcctgata atgatcatga
     6121 gaaagaatcg atcctccaac aattggtaag tcagcattcg aaccaacaaa gtaatgaggc
     6181 aagacttcag taatccgaag cagtctagca aatgtttcct ttgtgatttt catcgggcgg
     6241 tgttgttctg ataaaatgat cgaatgttca ttgtaataag cataagggga atattggaat
     6301 ccccagcttt caccgtctag attcattcga ataatccggt gattggtgcg ggctggatag
     6361 ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca tgcaggtagg atagtctact
     6421 tttaccgcct cgcgttctgc tgcgatttgt tttggatctt tttctggctt agataaattg
     6481 attgtgattt ccagttcacc atactccgac tctgccggaa atttgatgtt ttttgcgatg
     6541 gctcttgttt tgatgtagtc atttttctta cttaaattaa agaaatactc agttgcttcc
     6601 tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg
     6661 agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca
     6721 ccattttctc ttgccttagc gacaagctga tccaataagt ctacagattc ttccttaact
     6781 ggtcttactt caatgctttc tagagcatct tctcctacca tccctaaaag acgattctgt
     6841 aaatataagc ggtccatttc catccatccg ccagcttcga tcgcaagtgt ggcaaaatca
     6901 caaatcgttt gactgattga catatctcat tcccctattc ttcataacca tttggatggc
     6961 tgacatgcca atcccatgct gttttgataa tcgctttgat atctgtgtat tcaggtttcc
     7021 agcccagaat ctctctcgct ttttcacttg aagcgactaa tcggctagga tctccagctc
     7081 ttcttggtgc tatttctgct ggaatttctt ttcctgtcac ttctctagct gcttctagca
     7141 tttccttgac tgagtatcct ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt
     7201 ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa atcttctact tgaacataat
     7261 ctctgataca tgttccatct ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc
     7321 tttgaccaag agctacttgt aaaatgattg ggacaagatg cgtttctggt gtatgatctt
     7381 ctccgataga tgcatctgct tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc
     7441 gcatgccata tgcttggtca caccacttca tcattttttc catcatcaat ttactttcac
     7501 catacggatt tttaggatta gctggtgtat cttctgtgat tggagactct ttcggctctc
     7561 cataagttgc tgcagtagaa gagaatacga ttttattcac gttgaactcg tgcatgactt
     7621 caagtaagat ttgcataccg tatacgttgt tgttgaaata catcaatggt ttttcgacag
     7681 attctcctac taaagagctt gcagcaaaat ggattactcc ttcaatcggt tccttttcaa
     7741 atacggaacg tagaaattct ttatcccgga tgtctccttc ataaaaatga gcatctggat
     7801 gtactgcttg tttgtgacct gtgagcaaat tatctaccac agctacttga tacccctttt
     7861 gaactagctg gtctacagcg tgcgaaccga tataaccggc tccgcctagt actaaaattg
     7921 tcataaaaaa cactcctttg attagtaaaa ttgtgtttgt tgattctata taatttaaaa
     7981 aacgaagttt acttttatgg tgaaaacaaa caaagtaaaa tttcgtaaat tcatatggta
     8041 aatttataat aaaacattta gtaaacattt tcaagggttt tttgcaaagg tttacaaaca
     8101 aacgtaaaac tagccttttt ttctcttttg ttccataata gatataacaa ataataaatg
     8161 ag
//
LOCUS       DARDHU010000108         7902 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000108 DARDHU010000000
VERSION     DARDHU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.109"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..1032
                     /locus_tag="SDD03_002414"
     CDS             169..1032
                     /locus_tag="SDD03_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4304837.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1161..2783)
                     /locus_tag="SDD03_002415"
     CDS             complement(1161..2783)
                     /locus_tag="SDD03_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4304838.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3032..3553
                     /locus_tag="SDD03_002416"
     CDS             3032..3553
                     /locus_tag="SDD03_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG4304839.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3739..5469)
                     /gene="spxB"
                     /locus_tag="SDD03_002417"
     CDS             complement(3739..5469)
                     /gene="spxB"
                     /locus_tag="SDD03_002417"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG4304840.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5634..6452
                     /locus_tag="SDD03_002418"
     CDS             5634..6452
                     /locus_tag="SDD03_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG4304841.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6555..6752)
                     /locus_tag="SDD03_002419"
     CDS             complement(6555..6752)
                     /locus_tag="SDD03_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304842.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6921..7499
                     /locus_tag="SDD03_002420"
     CDS             6921..7499
                     /locus_tag="SDD03_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG4304843.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7594..7731
                     /locus_tag="SDD03_002421"
     CDS             7594..7731
                     /locus_tag="SDD03_002421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304844.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2368 a         1408 c         1564 g         2562 t
ORIGIN      
        1 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
       61 tattttgatt agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg
      121 ctataataga cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt
      181 gtaacagatt cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt
      241 ttatcaatga atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc
      301 aattatgacc gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc
      361 aatgttggcc gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta
      421 tatgtggctt tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa
      481 ttgctgaagg aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt
      541 attggggaag ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc
      601 gaagaaaccc tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta
      661 gacgatctga aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc
      721 agtctattga gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt
      781 gacaaagttc gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca
      841 atcgttgcac cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg
      901 gagaaggcaa aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc
      961 ttaggaccaa cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga
     1021 atcagtcgat aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa
     1081 aacagcgact cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat
     1141 tttttaagct aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca
     1201 ttttccaaaa attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg
     1261 gcaatgatgc ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt
     1321 gagccagtaa atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg
     1381 ttcgttggat catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg
     1441 caacggactt tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa
     1501 aacaacattc ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca
     1561 tattgacgca accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt
     1621 ggtaaataag aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt
     1681 tcacccatga taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg
     1741 accttgtctt ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc
     1801 gttacactta cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat
     1861 ccaacgaatg gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc
     1921 atttttttac gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata
     1981 aattcttgga gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta
     2041 gccgctagtt gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta
     2101 ccaaaatcta gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat
     2161 acaacgatca cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg
     2221 atatccagac cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt
     2281 tgtgctaata acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc
     2341 aattcttcag ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat
     2401 ccattaagct cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg
     2461 aaatcctctt tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt
     2521 cccatgatga ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat
     2581 acggccatcc gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct
     2641 gataacactt taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag
     2701 tttccggggg taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa
     2761 ctcacatcat ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa
     2821 gtcttataga attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg
     2881 tttcacgtga aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata
     2941 ggatttatta gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa
     3001 aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg
     3061 ctcattgatt ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt
     3121 cggaaattta gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt
     3181 tgaactgttt gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt
     3241 aataatcggg atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg
     3301 agtcgcagca ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt
     3361 atttactgct gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt
     3421 caatatttta ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt
     3481 gacattaagc tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta
     3541 tgcatttaga taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact
     3601 gttgtaattg gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt
     3661 gtcgagacaa atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact
     3721 cgaagcatag cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc
     3781 atggacttca tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc
     3841 taaatgcaga tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac
     3901 aggacgatct gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac
     3961 tgtaaagcta tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa
     4021 tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc
     4081 attcgaaaag acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg
     4141 catgaccata gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct
     4201 tagttgtgcg gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt
     4261 cgtatgtttt tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc
     4321 gacatctgtg acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc
     4381 ctctggacga atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt
     4441 ttctttattt tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc
     4501 gacaagtctt tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg
     4561 tcgtccaaat tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa
     4621 tgcacgtcca aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc
     4681 ttcatttccg ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc
     4741 aggaataatt ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa
     4801 ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt
     4861 tgctttttca agataaggca aagcagctag caaatctttt tcttctggta atatcacact
     4921 tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc
     4981 aacagggata gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc
     5041 tactacatgc ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc
     5101 gaaaatagga ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac
     5161 ttgtcctaag aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat
     5221 caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt
     5281 tgcatctgca gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat
     5341 ttcggttttt tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc
     5401 gtaaatatgg tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat
     5461 ttttgacatg gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag
     5521 tatagaactt tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt
     5581 ttttgggaat atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg
     5641 aatggctatt gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt
     5701 ttatgacagc tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc
     5761 taaacttgat gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt
     5821 tagatccggc tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta
     5881 ttgggtttgg tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc
     5941 attttggacc acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc
     6001 ttgttttttc tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg
     6061 gtgcagcagc aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc
     6121 taggtattgg tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag
     6181 aaggtctgag tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa
     6241 ttgctggagt gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac
     6301 ttgcttttgc cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac
     6361 agcagacatt gtccagcaaa cgtcattttg cagtttttgg cgtcatgagt ggatttatca
     6421 ttatgatgat tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa
     6481 tagggccgcg aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg
     6541 aatggtatca tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca
     6601 agccagatga taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt
     6661 aggacactga caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg
     6721 aagatattta agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta
     6781 ctatagaaaa agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta
     6841 cttcatctac ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag
     6901 cggaaaagga ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa
     6961 tcgcgattat catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg
     7021 aggcgctcgc tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag
     7081 cgatttattg gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg
     7141 tcttatgggc gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat
     7201 ggatcagtga gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga
     7261 ccgctaatat tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg
     7321 agaaagcatt gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga
     7381 atgtgttagg aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca
     7441 tcgtgattct agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa
     7501 atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat
     7561 ccgagtgata ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga
     7621 ctgattacta gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca
     7681 gtagttggtg tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta
     7741 tgtaatctga tgttctatct atttaagaaa agcatgttta cagcagtatg attctgttgt
     7801 aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag
     7861 ttagtttttc cacagtggat aacttcgtaa gagataaaaa ta
//
LOCUS       DARDHU010000109         7840 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000109 DARDHU010000000
VERSION     DARDHU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.110"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..995)
                     /locus_tag="SDD03_002422"
     CDS             complement(21..995)
                     /locus_tag="SDD03_002422"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG4304845.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="SDD03_002423"
     CDS             complement(1075..1950)
                     /locus_tag="SDD03_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG4304846.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="SDD03_002424"
     CDS             2080..3987
                     /locus_tag="SDD03_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG4304847.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="SDD03_002425"
     CDS             complement(4528..7806)
                     /locus_tag="SDD03_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG4304848.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2208 a         1659 c         1461 g         2512 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt
//
LOCUS       DARDHU010000110         7777 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000110 DARDHU010000000
VERSION     DARDHU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.111"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..333
                     /locus_tag="SDD03_002426"
     CDS             <1..333
                     /locus_tag="SDD03_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304849.1"
                     /translation="VLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSI
                     SLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNN
                     FYFYRNKL"
     gene            complement(459..1136)
                     /gene="nth"
                     /locus_tag="SDD03_002427"
     CDS             complement(459..1136)
                     /gene="nth"
                     /locus_tag="SDD03_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG4304850.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1143..1841)
                     /locus_tag="SDD03_002428"
     CDS             complement(1143..1841)
                     /locus_tag="SDD03_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG4304851.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2109..3293
                     /locus_tag="SDD03_002429"
     CDS             2109..3293
                     /locus_tag="SDD03_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG4304852.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3299..3742)
                     /locus_tag="SDD03_002430"
     CDS             complement(3299..3742)
                     /locus_tag="SDD03_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4304853.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3968..4255)
                     /locus_tag="SDD03_002431"
     CDS             complement(3968..4255)
                     /locus_tag="SDD03_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304854.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4426..5778)
                     /locus_tag="SDD03_002432"
     CDS             complement(4426..5778)
                     /locus_tag="SDD03_002432"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG4304855.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6051..6497)
                     /locus_tag="SDD03_002433"
     CDS             complement(6051..6497)
                     /locus_tag="SDD03_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4304856.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6733..7659)
                     /gene="cysK"
                     /locus_tag="SDD03_002434"
     CDS             complement(6733..7659)
                     /gene="cysK"
                     /locus_tag="SDD03_002434"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG4304857.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2105 a         1582 c         1270 g         2820 t
ORIGIN      
        1 gtacttttaa gcactactct taaaggaaaa tttatggaaa gaagctctca tgcaatgatt
       61 ctcacttttt tatggttgtt gttcaccaga atctatattt ttctgatgag cctgaaaccg
      121 cccaaatcca ttagtttatg tttagttata aatggtattt ttttattatc acttatcatt
      181 tttattacac ctttagatag atacgtcact tatttatata atcctttgga atactggact
      241 tattttatag gtatattgga aggcattatt ttctacatcg ggtattttcc caataaaaat
      301 aacaactttt atttttatcg caataaattg taataagtta ccatcctagc tactgagtat
      361 gtacatttac tttttcatgc aaaaaagata aacgaaacag cttttctcca taataacaag
      421 ataattcata catttatttg caattaaaga aaccacgtct acaggcgtgg tttctttttt
      481 agcgcctttt cttttagacg catccgattt ttcccatctt gacatattga tagcaaagga
      541 catacctcgc acttgggatt tcttgccgtg caatgatatc taccaaaaaa aatcagtgta
      601 tgatgtgtct ttacccataa ttcttgtggt actttccgca tcagtgtttc ttctacttcc
      661 atgaccgtag catctagttt gcagatcctc aggcgttttg agacacgttc cacgtgggta
      721 tctactgcga ttgctggtat cccgaatgca tcccccaata cgacatttgc tgtctttctg
      781 cctacaccag gaagagacat caattcttct cttgacgttg gcacttgccc atcgaaacgt
      841 tcaatcagct gctgtgcaca cgctttgatg ttttttgctt tatttcgata caaaccaatc
      901 gtttttattt taagaatgat ttcatcgatt gaagcttctg ctaacgcatc gggtgtcgga
      961 aaagaggcaa acaaatctgg cgttgcttta ttcactgata catctgtcgc ctgggcactt
     1021 aagatcactg caatcaataa ctcaaatgga ttattgtgct tgagttctcc atgtgcttcg
     1081 ggaaacattc catacatcgt ttctagagct tccattgttt tttgtttgct taacatattc
     1141 cgctactcct ccaaccaatt tttcagtgag actttcggca attcttcttg ttgagctgac
     1201 tgttctactt ctctttgaag aagctgctgt tttcttcgtt tttggtcttc ttccacttgt
     1261 tgttttgtac gtagattttt tctttcccac gatagcaaaa tgcggtcaac gtaattaaaa
     1321 ctataagcct gatttaatac ggcttcccgc agtgctagct tcaaaatttc cggctgataa
     1381 tggtcttctt ctagccattg tccgatccgc tggaactcga tcgaagaaag cgggcgtcca
     1441 aattcttgtt caaataattg atacactgac tggatttctt tttcttgtgt tttttcttcg
     1501 tatttttttt cttgtgtttt taaatactca cctaatagat catagatggg gtaaagatta
     1561 tatcgatctg cttttttacc gttgacaatc gtcgtttcta tcttgataaa tccatttgtt
     1621 acgagacgat ccagtatttg aaaaatctga tcttgtttga ttcccatatc catagcaatc
     1681 atctgcaaat cagggaaagt atctccttca agctgtgcca tatgcaactg tagaacaaat
     1741 aaaaattctt cagattgtag accgatccta tgataatttt taagtaaaag attagatatg
     1801 actgtttgtc cagcgcttaa gtaattttct aaattcaaca tgatttctcc tccgctcaat
     1861 cacagtatac gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta tttacttcac
     1921 tgatcggtca gatttacaat gtcgtctact tgatttttgt ttgttcatgt ttttgatgcg
     1981 tatcttcact tttcggaata aataaattaa gtagatttcg ctgttcctta gaatgttgac
     2041 aaggaaacaa aattactata cactattttt gtttccaagt taacattatt gcataaagga
     2101 gtttttacat gtacagtttc acaaataaac attctaaagt ttttggatac gtctctgtct
     2161 tattcactgg attaggattt actatcgtca gtcctgtact tccgttttta acgctgcctt
     2221 attctcactc catacatcag caagctttct atatcacttt gctcatgtct gtttatgctc
     2281 tagctgcctt cttatctgcc cccattctcg gtagcttaag tgatcatttt gggcgtcatc
     2341 cgatccttat tatcagttta ttaggatcaa gtatcggata tttgcttttt ggtttaggaa
     2401 attcgatatg gatgttgttc cttggtcgta tcattgaagg attgacggca ggagaaatct
     2461 ccaccttata tgcttacttt gccgacatca cggaacctaa tgaaagaacc aaagtttttg
     2521 gatggatggg tgctttagtc ggtataggaa caacgctggg tcctattata gggggtctat
     2581 tagctgagtt ggggaattct gttcctatat ttattggagc actctttact ttcttaaatg
     2641 ccgtttatgg ctacacattt atgctagaaa gccttccaat aaaaaaacgt tcagtagatt
     2701 taagtttctc ccatgtaaga ccttttcacc aattacaaca attattcaag atttcatccg
     2761 ttattccttt attgacagca ggttttgctg tttggttggc agctggctct cttcaatcta
     2821 tcttttctca attttcgatt gacgcctttc agtggaaggc tgggttggtt ggtttaagct
     2881 tctctttgat tggaatcttg gatactgtct cgcaattgtt cattatgcct cgattgctca
     2941 aaaaattttc agaacagcag atcacaagaa tagggatgtt cagtgaaatc ctcgcctatc
     3001 tattcatcac attatctgga attcttctac tgccgatttt gtttttattt gggattatct
     3061 gttatggttt cggtgattct atttttactc ctgtattcaa tggccaacta tccaattctg
     3121 tctcagataa ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa tctctatcgc
     3181 gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc agcaccattt cttccgacgg
     3241 tattcggatt tttgcttatg atttttgcct attatcacta caataaaaaa tagtagcttc
     3301 atttgctact tcgtataaat tctctgtatc gaccgataaa acgaaatact gtgtcaaatt
     3361 cttcttttga catattttca aatacagtag catctcgctg tacccatttt tgatggagtt
     3421 gctcgtgtct attccttatt ttttgtccga ttggcgtcaa ttgaaaatag atttcttttt
     3481 tattctcttc gctttgataa gtgtttatga tccctctagc tatcagtttt tttgttagct
     3541 tactgatggc tcctctagtc atgtaactag cattcgctaa ttttgtcacg ttaggatcag
     3601 ggattttccc gatatgttca atcacttcta gttcatttag agtaaatcct gcaaattctt
     3661 tttccatttc tggtttatta cgatgaataa tctcattagc taaatccaga tattcttcta
     3721 ttagcagttg tcttttatcc atttctacac cttctttttc tttcattata ttgttttttt
     3781 gtttcctatt caacaaagat aaaaatattt tgtaggaaaa cccaatctga aaccaagaaa
     3841 aaaaggggtt gacaaagctt gtcataaccc ctttttctga ttaaaagata gtaaagagac
     3901 agtcatcggt aattgtgatg attttttgtc atcacttaag gatgagcggc cttttcaaaa
     3961 actgtcttta aagatcatca tcagtttcta gcacatatgg ctgaccttca tatgtgtact
     4021 ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg tctactgaaa ggaaaacgat
     4081 gagaaacatt atctttggcg tcatttgctg gcatcgtgta atattcctct tgcgtttcat
     4141 tcaactgaga gagtacttga tctgccaact catcaatatt gatcgttaat ttcttcgaag
     4201 cacttttata atcttctaaa aatgcatctt gttggacaaa atattcttta ttcatcttct
     4261 ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt cttttatgta acagctttat
     4321 ctttgctgct tattgtgaca aaaaattcga agaaagacaa ctaattgtac ttttcacaaa
     4381 aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt tttctttatt gtgcagtttc
     4441 gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa ttccatgggc ggtcaaaatg
     4501 tggttggaag aagaaatctg aaagtgcgag atcttctaca gtcattttat tttgaacagc
     4561 taaagacatg gtatttgctg attgtgtaat gtcgtatttg gacatgaact gagcacctac
     4621 gatacgattc gttccttttt catagactag ctccatcatt actttttcag tagttggcat
     4681 gaactctgga cgataattat cttcaaatac agtcgcttgg acttctagat cgttcaaagt
     4741 tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag ccaaataggt aaagtcctga
     4801 agttccttga gtaccacgat acgccatttt atgatctgtc aagttatttc ccacaagcaa
     4861 accttgacga acggcatttg ttgccaaagg aatatagttt tgcgtgccac ttggattgta
     4921 gttgactact gcagagtctc cagctgcaaa aatgtctgga ttacttgtct gcatgtattc
     4981 attcactttg attgccccgt ttggcagtgt atctactttt ccttcgatca gttttgtatt
     5041 tggacggaag cccacacata aaatgaccat atctgtatca aatgtatctg ttggtgtagc
     5101 tacttgtttc acttttcctt cttcatctgt gataaattca gtaacatttt cgcctagcgc
     5161 aagagtaaca ccgcggtctg tcaattcttt ttctaagata tctgtgaatg gtttatctag
     5221 atatttattc aagatgcgat ctaatccatc aatcaatgtc acttgttttc cagattcgac
     5281 aaaagcttct actaattcaa taccgatata tccaccacca acaattacta cacgttttgc
     5341 ttctttcgct tgtgcaataa tttcatttgc ttgattgtag tttttgcata ggagtacatt
     5401 tttcgcatcg attccactaa tcggtgggat aataggccat gaaccagttg tcatcactaa
     5461 tttatcataa gaaaccgttt ctgtttctcc tgttttcaga tcttttgccg ttactgtttt
     5521 attttctgta tcgacgtctg ttacatcgtg ttccatttta actgttgctc ctaatgaaga
     5581 taattcttca ggatttgagt agaacaagcc tgctgggtcc tttactactc cgccgacata
     5641 aagtgcgata ccgcaagata agaaagaaat attgtcattt ctttcatata ctgtcacttc
     5701 tgcttctgga tgatttttta aaatactttt tacagctgca gtacctgcat gagtacagcc
     5761 aattactact actttcattt atggtccctc cgattaacat tttgtccttc gacaaaacta
     5821 ctataacaga aataaaacgg atacaacagt ttaatgcttg tgaattcaca aaggtttgtt
     5881 tgtagtttca ttccttttca agcataagaa cccaatatct acaagattct tattttaata
     5941 atattcactt ttatatctaa gattgtgaat aaaagacaaa aaaataaaaa agctagcgtt
     6001 ttttttacac agctagcttt ttttaggtat aacacttgta gaaactgttt ttacatttgc
     6061 gccttttttg ctgactcttt acaatccgga cagagtccat acaattccaa ccgatgttca
     6121 ttaatctcaa aatcagtcag tctgcttgca gccatttcta tatcttcaag ccctgggtaa
     6181 tgaaaatcaa caatcttacc gcagttttca caaattacat gataatgacg ctttgaactg
     6241 aaatcaaatc ggctagaagc atcgccgtac ttcatttctt gaacaaatcc aatcgaagta
     6301 aataagcgaa ggttattgta aacagtcgca acactcatat ttggaaaccg atgttcaagt
     6361 gctttataaa tctcatcagc cgttggatgg gaatgatgtt caatcaaata ttctaaaacc
     6421 gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta acttttcgat aacctcttcg
     6481 actaacttat tttccatgta aacaccccac gttgttattc tcgtcttatc atactatttt
     6541 tacattttaa aatccactta acatgaaaag aaaattcaat tttattgtat tttaatgaaa
     6601 aaagacgtaa aacagtcgcg ttcactattc tacgtctcta ttcaaataat ttttctcatc
     6661 ataaattttt gtgtgttctt ttcatcttta aaagtttttt acataaacct atctggagaa
     6721 aaaccaagtg ccttatatct cttggtaaag ggctgtagat aaataacgtt caccattgtc
     6781 gggaaccaca gctaatacct tcttgccttt tcctaattct tttgcgactt ttagcgcggc
     6841 agcaattgca gcgccagatg agatgcctac taaaatccct tcttttcgcc caacttctct
     6901 agctgtttcc atcgcttcgt ctccactaat agaaaggact ttatcataca cagaagtatc
     6961 cagtgtttta ggaacaaaac ctgtaccgat tccttggatt ttgtgaggtc ccgcttcttt
     7021 cccttctaaa atagcagatt ctgctggttc taccccgatt aattcgattt tcggatacac
     7081 acgttttaat tctcggccag cgccagtgat cgttcctcct gtaccgatac ctgcaacaaa
     7141 cgcgtccaag ccatttactc caaatgcttg atgtatctca ggaccagtcg tcttctcatg
     7201 cacaagagga tttgcttcat tttcaaattg taatggtaaa aagtagccgt tttcttttgc
     7261 caaacgttct gcttctctga ttgatccaga aataccatcc gcaccaggag tcaaaatcaa
     7321 ctctgcgccg taccctttca tcaataaacg tctttcgata ctcatcgtct caggcatcac
     7381 gatgattact ttatatcctt ttgcaactcc taccatcgac aatccgattc ctgtatttcc
     7441 agaagtaggt tcaatgatag tatcacctgg tttcagtaga ccatcgtgtt ctgctttttc
     7501 tatcatactt aaagcaatac gatcttttac acttcctcct ggattaaaaa actcaagctt
     7561 tacaaaaaca tctgctgaat cttctggtac gattttattt aatttgacga ttggcgtttt
     7621 gccaatcaat tcggtgactg agttatagat agctgtcatc ttcaattcct ccttgtcttt
     7681 gctcttctta cttatcataa tacaatctgt tcatttatgg cgagtgattc gtctttgact
     7741 agcaaaaaaa agacaaagat ataccatata tggtaat
//
LOCUS       DARDHU010000111         7720 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000111 DARDHU010000000
VERSION     DARDHU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.112"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..787)
                     /locus_tag="SDD03_002435"
     CDS             complement(59..787)
                     /locus_tag="SDD03_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4304858.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="SDD03_002436"
     CDS             complement(800..2080)
                     /locus_tag="SDD03_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4304859.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="SDD03_002437"
     CDS             complement(2221..2823)
                     /locus_tag="SDD03_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG4304860.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="SDD03_002438"
     CDS             3068..3274
                     /locus_tag="SDD03_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304861.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="SDD03_002439"
     CDS             3586..4305
                     /locus_tag="SDD03_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4304862.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="SDD03_002440"
     CDS             complement(4386..5069)
                     /locus_tag="SDD03_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG4304863.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="SDD03_002441"
     CDS             5287..7473
                     /locus_tag="SDD03_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG4304864.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2772 a         1297 c         1446 g         2205 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggtgtgac
     7621 aagaattttg tcacacctcc cttatttatt ttgaaaatga gaatactatt atcaaaatag
     7681 agaatatata gaaaaattct cattattcaa acgtaaataa
//
LOCUS       DARDHU010000112         7664 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000112 DARDHU010000000
VERSION     DARDHU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.113"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..621)
                     /locus_tag="SDD03_002442"
     CDS             complement(<1..621)
                     /locus_tag="SDD03_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4304865.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNH"
     gene            complement(621..1757)
                     /locus_tag="SDD03_002443"
     CDS             complement(621..1757)
                     /locus_tag="SDD03_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4304866.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1789..3240)
                     /locus_tag="SDD03_002444"
     CDS             complement(1789..3240)
                     /locus_tag="SDD03_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG4304867.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3284..4048)
                     /locus_tag="SDD03_002445"
     CDS             complement(3284..4048)
                     /locus_tag="SDD03_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG4304868.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4076..4774)
                     /locus_tag="SDD03_002446"
     CDS             complement(4076..4774)
                     /locus_tag="SDD03_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG4304869.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4786..5565)
                     /locus_tag="SDD03_002447"
     CDS             complement(4786..5565)
                     /locus_tag="SDD03_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG4304870.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5581..6525)
                     /locus_tag="SDD03_002448"
     CDS             complement(5581..6525)
                     /locus_tag="SDD03_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4304871.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6571..7350)
                     /locus_tag="SDD03_002449"
     CDS             complement(6571..7350)
                     /locus_tag="SDD03_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304872.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2162 a         1615 c         1173 g         2714 t
ORIGIN      
        1 atgattttta gaatatgaaa ttcttccaat atgtcccatt acaaacgtct gacttgaaat
       61 tcctaatttc attcgtacat catttctaat actttcatca taataaaatt ttttagcatc
      121 cactgcattt ggaatcaaag tgaatttatc atttcgaaac aaaaaatcac ctgctacttt
      181 tccacatgaa aattcatgtg tcaaaacaga cgttaactta catcgataat attgctttaa
      241 aagatattta aaatctctat ctatcgatgt attatggcta tgtgctatcc gtatcggaat
      301 cccagctttt tttgcagctt ttaaaggtaa ataactcatc gcatcaatat gagaatgaac
      361 aatctcatat tctggatgat ccttaaaaaa ttgttccatc catttaaaat atttaggata
      421 attttgtgga taaagtctcg gtgcataata gacctttcct ccaagactta ttatttcctc
      481 atcataatca cttttatatg gacgatgtgt caaaaaatca aactgaattt tagttcgatc
      541 tatattccta taatagttca ttagcatcgt ctccaaccct gcacgatgca tatcattcac
      601 acactgtaat attcgaatca ttatatattt cccctataca aatttaatgt ttcttgtacg
      661 attttatccc aattatattt actacaaata aactcagtag cttgattctt caaaacttta
      721 accatttccg attgattgca tgcctcttct aatcttatct ttaaatcact tacatccgat
      781 ttcttaaaaa tcatagcctt gtcttctact acttccgtac actctgagat atttgaaact
      841 atacaacagt tcccataact cattgcttct aacaaactta atggcattcc ttctaaatca
      901 cttggaagtg tatagatata cgcattacta tataattctt ctaattcctg tccttgtaca
      961 aatccagtaa atataattcg ctcatcacct tttgctaatt cctttagttc atttgcaaat
     1021 tcatctgtat cagagcttcc tccggctatt ataagttttt tatctgtctg aacatcttta
     1081 aatgcctcaa ttagataccg aattcctttt tcgggaacca atcgccccaa aaacaaaata
     1141 taagaatctt ttgttaatcc atacttttct gtaatcaatc ctgctgattt tatttcaatt
     1201 tttttaacgc catttggtat aaaccttgtt tttcttccat atatatcttc aaaataattt
     1261 tggacgcttt cactaagtac aattatttca tctgcaaatt ttacagcaca cttttcccct
     1321 aacatgatgt atgtactagc aagcttgttc catttttctc tttgatgatc tagaccatga
     1381 attgttgcaa cacatctttt cccaaaaagt tttggcaacc acaacatagc acatggtcct
     1441 tctgcatgaa aatgaacaac atcatatttt ccgaatgctg ctctaattcc accaaatact
     1501 gaagaactca ttgctgcaat tccttttata tttaatgtaa aaatagtctt taattttaca
     1561 cctttatatt ctttattttt atgctggtca aattcctttc cgctaacatg atgaccactt
     1621 cgattatagc aagtcacttc atgcccaagt gcaaccattc gcgttgccaa ttcttctacc
     1681 actatctcaa ttccaccttc tcttgatgga attcttttat gacctagcat acagattttt
     1741 aatttttttc ttgccatcac gttttactcc ttctatagta tctttatctt acatggaacc
     1801 gtttctatga agcacagttt tgactgtcct aaacaaaatt ctaaaatcta atcccatact
     1861 ccaattcctg atatattctg catcaagttt tacaacctct tcgaagtctg tgatatcact
     1921 tctaccactc acctgccaca ttccagtaat cccaggtttc atcgcaattc tcgcacgatg
     1981 atgaaaatca tacttctcat attctgactt caagcaaggt ctaaacccca ccaggctcat
     2041 ttttcccatt aggacattat aaaattgtgg aaactcatcc aatgatgtag atcgaataaa
     2101 ttgtccaatt cctgtcttct ttgttccatc aggaagtatt ttattgccaa tgattcttgg
     2161 gtcaaaatcc atcttaaaca tgagattatt actcatctta ttttctttca tcaactctgc
     2221 tttgcgttct tctgcatcca tatacatact acggaactta tacatcttaa atgtcttacc
     2281 attttgacca atacgttctt gtgcaaagaa aatcggccct ggtgatttga tgtaaataac
     2341 tggtgctacg aagataaaaa taacacctgt tgcaatacat ccaataattc cagcaagaat
     2401 gtcaatcgtt ctctttataa atgcttgcct catcgtcata acattaatac tagatgtaat
     2461 aacagtataa tcaccaactc ggccaataaa ttgttttcca cgtgtactat cagacacttt
     2521 tgtaagattc atatgtacag caagtcccat ttccgaacat ctggcgatta attctttggg
     2581 atatggaaca ctttcgtcaa cattgatgaa tacttcatct acccaattct gtaaaagata
     2641 ttccgctgca ttatcagcat ttgcaactac tgggacacct ccgatggtag ttccatacat
     2701 atctctgtct actacaatca caccactaag tttatacatg ccataattct tctctttaag
     2761 attacgaact acattttttg caatcttttg agatgttaca ataactaacg atacatcttc
     2821 accgtttttc atgcggtgat gcaacaaccc tttccaaagt atacgaatta tatatgttaa
     2881 taatccatac accaccccca ttataaataa agaaactctg gaataatcat ttccatcctg
     2941 cattgtaata aggtataaac tgcacacaag taacagcata aaagattgct ttatagaagc
     3001 tacagcctct ttataatatc ctctttttaa aacattttta tatgcctcaa ataaaaatat
     3061 aatcgtaaga ttctcaagtt ctataaaaat tgccatgtgg cgataaatag gaatcacata
     3121 cggattccag tcaccctgtc gcattacata tgatatataa aaagcaagct ggacacaaaa
     3181 taagtctaat aaaataaaat caatatgttt agaccaacca cttgagtttt ttctatacat
     3241 ctctcactct ctcctcattt cctttttatc tacgaataaa gaactaaaat aaaccaaact
     3301 tcttcttttt tacctcttca tagattggat aattgatctc atccccgttg atcagatcct
     3361 ttgcgacttg tttcatctgt actacttttt ccttaccaaa ctccttggca atctgctcat
     3421 agcactcttt taagtaaaag gtgcgcttag agaccccgtg tgcatcagaa gcagccatct
     3481 gcacgagatt atgtttgacc attgttttgg ctgttttctg gatatctttg ccaaatacac
     3541 caacataact tggcgcagta agttgggcca aacatcccat atctaggaac ggaagcaaat
     3601 gatttgggtc ttctcgaaaa tgcgcgttgc gttctggatg gacgatcact ggtatctttc
     3661 ctagttctaa caactcaaaa aaaagccgtt ctgcatataa cggcacttcc agtgtcggaa
     3721 attcgatcag aagatatgta tcatctaaat ctgtaaataa aatttcatct cggtggatgt
     3781 cttcgatgag cgttccggta atacgtactt cttgtccttc caataacgtc aaaggcaact
     3841 gtcttttctc taattcggct tgtaacgaag cgactagaga aatgacttgt gatttttcat
     3901 tgctgtattt tccattattg tggtgtggtg tgcataaaat atgcgtaatg ccttgctgaa
     3961 tcgctttttc agccattgca atacttgctt ctaagttttc tgccccgtca tcgattcctg
     4021 gtaggatatg gcaatgtaga tcgatcattt ctcttgtctc ctctcttctt attccttaat
     4081 ttccgtagta ataaccagca tctttgctgt gttctgctcc gttgtatacg acacctaaga
     4141 ctcgtgcttg taccatatct aacagttctt ttgctttagt caatgattct tttcgtgacg
     4201 tattttcccg tacgaccaat aaggtgccat ccgtttttga agacatgatc tgtgcatctg
     4261 ttacggcaac gactggcggc atgtcgaaga tcactacatc aaatagttgt cttgcttctg
     4321 ctaaaatctg atccattctt ggcgaactta gtagttctga tggatttggt ggtttaggtc
     4381 cacttggcag aatcgacaaa ttgtcgactg gtgttcgttg gatctcatct gctacacttc
     4441 ctgatgaact aagaacagta cttaatccgc ttgcgttgtt caacttgaac gttttataga
     4501 tgacgggctt acgtaagtct gcatcgacta gcagcacccg ttgaccagat ttggcaaaaa
     4561 caacggcaat gtttgctgca gtggtcgatt tcccttctcc aggtcctgat gaagtcacaa
     4621 caatcgtttt gatttgctga ccagctgcgg aggcaaactg gatattcgtc cgaatcgtac
     4681 gatattgttc agcgattggt gaagagggat tggtgagtgt gaccaaactg actgcatgag
     4741 tttgggctgc tttttgtttc tgtgttcgtg ccattttctt tcctcctaga ctcttgagcg
     4801 gcttctgcgt gatggcgctg ttttatcgtt tgtagctttt ggttgtgtct gagctggtcc
     4861 ttttttacgg ataccagcat tcaattcttt cgctgacatt tctggaactg ttcctaagat
     4921 tgggaatcct aatgtatctg tcacatattt ttcatctttc actgtccgat cgagtaattc
     4981 caataagaat gccagaccta cacctaccat cattcctaac attaagccag tcattaaatt
     5041 caatttatta tttggcgaaa caggtgtcgt attggcaact gcacctgaaa tgatcgagat
     5101 tttatcaaca ttcatcacat cttttgcgtt ctcttggaaa acttgtgaag ttgtgttagc
     5161 gatctgttga gcagccactg cattcgtact tattgcctga atcgagaaca tttgtgaatt
     5221 ttctgcctga ttaactgaga tggcatcttt gatttgttca gcagtcattt tcacattgtc
     5281 ttctgtttct aaacgatctt tgacttcatt gagcactaaa tcactgacga tcatatcttt
     5341 atacgtattg atcatttgca ggttcgtatt gacatcattg acatttgcag tgtctgattg
     5401 tggcagtgtc acgatcaatt gtgcttgtga actgtatttt ggtgtcaaga tgaaaaatgt
     5461 tacgattcct gagatggcca agccagcaaa cattgagatg atgattatta ccaagtgttt
     5521 gcgtaatgtt tcaaaaattt ctttcaagct gattgtctct tccatttttt ctccactttc
     5581 ctatttattc tctagattca tttgatcttt taattctttt ttcacacgat caagttcttg
     5641 atcactgact ttttgatagg agatattatc ttgcatgaag ccttctcctt gcagctgatc
     5701 ttgcttaatc gtaccaaatg cctcccgata atctaaagca atttttttca tttcatcaaa
     5761 gttcatgtct gttttcatat tcgactctag agcattcaaa acggattgat agttgctgac
     5821 accgtttaga ctcaatactt ttttcgctac gccttctacg atctttcttt ggcgcccttg
     5881 tctaccgtaa tcaccatttg ggtcttcata acgcatacga gaataagata atgcctgatc
     5941 cccgttcaat gtaattttac cgattggaaa gtcatagcca tcttgtgaga aagtgaggtc
     6001 attattgact tcgataccac caactgcatc aacgagttcc tttagccctt tcatattgat
     6061 agagacatag tgatcgattg ggatgtctac atatttttgt acggtatcca tcgccatggc
     6121 agctccgccg aatgcgtaag cgtggttgat cttgtctttt gtgtcacgac ctacgatgtc
     6181 gacatacgta tcacgaggaa tgctgacgat cgttgtctgt ttgtcttcag gactgactgt
     6241 ggcaaccatc atcgtatctg atcttccttg atcgacccga cctaaatctc cagtatcaat
     6301 ccccatcaat agaacagaga aagattcttg tttgtccaga tcaactggtt tcgttcgttc
     6361 tccttgggtt cgttccactg attcgtaagt tttctcaata gaatgtgaga gatccagata
     6421 taattttgct cctactccta caattgctaa tactaatacg ataagaactg ctaaaataat
     6481 cagaaatatt tttttccctc ttgattttct tcttcgtctt gccatagttt cctccgtttg
     6541 ctaaccttgc ttgaatcaac gttttaactg tcaatagctt atattgctat agcccatttg
     6601 ctcagcaaca gtttgtgctt ttgttgcgta tgctgcgctt attttttcat tccggatctg
     6661 ctctatttga ctgagcaccg tcaaatattg atcgtaagta gcggtggcag tcacttgacc
     6721 atcctttaac atcgtatcga ataactgatc tagttgcgtg acttgcgcga tctgaatatc
     6781 agcgaatcct agatattgca taacaaccgt tttccaagga ctatctttaa aaaagctcat
     6841 attttcacgt acacgagcga catctgcact tgtcaccttt tctttgatga tgacatcgtc
     6901 tacagcttgc tgccagtttg atacattttt agtaaataaa tcgttgacag ctgtctggat
     6961 cttcagtttt gattctgcac tatctaactg tgtcacgatt gctttcttct catcttggat
     7021 tgcaacagct tctttaggca attctgattc tttgatgtcg taatcatctg ctgaaagttt
     7081 gacagaggat agcttatttg acaaacttgt gattgtttct tctgttattt cagggtttag
     7141 aaaaattttt tcttttgtac tatacagctt gtctacttct tttgataaag aagtcaaggt
     7201 gtctttttga ttggtgatgg cagattctgc ataattgatc cgttgctgat gttgccgctg
     7261 attattgata tggtatccaa acaaacagat cgcaatagca cctgcaccta ccgccacagc
     7321 agtcaatgac ttgacgaatg gcttttccat agattcactc ctaagtattg atgatagata
     7381 aattaagata taattcactt acataaaaat gacagctttt tatttaaaaa aacataatta
     7441 aactaatata ttcacagaaa tatttgtaaa caagttttcc ctatataatc ataccacaaa
     7501 ctgttttctt gactagggca atttacgatt taaaagtaaa taaaacatta ataagataaa
     7561 aattaatcga tttcattttt atttccataa ttttcatagg tgtttttaaa agttacacaa
     7621 aaaatagaat aacaaaaaaa ttaacattga attaataata gtta
//
LOCUS       DARDHU010000113         7358 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000113 DARDHU010000000
VERSION     DARDHU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.114"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..691
                     /locus_tag="SDD03_002450"
     CDS             <1..691
                     /locus_tag="SDD03_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304873.1"
                     /translation="CIFVLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYK
                     GDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSK
                     SNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERD
                     SYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKI
                     YKEIIEQYFPK"
     gene            874..3549
                     /locus_tag="SDD03_002451"
     CDS             874..3549
                     /locus_tag="SDD03_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG4304874.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3731..3970
                     /locus_tag="SDD03_002452"
     CDS             3731..3970
                     /locus_tag="SDD03_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304875.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3986..5359
                     /locus_tag="SDD03_002453"
     CDS             3986..5359
                     /locus_tag="SDD03_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4304876.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5363..6997
                     /locus_tag="SDD03_002454"
     CDS             5363..6997
                     /locus_tag="SDD03_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4304877.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7160..>7358
                     /locus_tag="SDD03_002455"
     CDS             7160..>7358
                     /locus_tag="SDD03_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304878.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2625 a         1142 c         1458 g         2133 t
ORIGIN      
        1 atgtattttt gtcttattat tacatggaag agttattaac ttagttaatc gaaaaagtac
       61 gactaaattt gcaaactcat tctggttttt aaaaaatatt aataaaacag tggaaaagaa
      121 tggagtttat aaaggtgatt ttcattttca aaatgctgag actattgaac gagaacatca
      181 ttatttcgat ttattagtga atacagtggt agaattagcg aaatgggcaa agtcaaacga
      241 aacttatagt caagtactta aaagaattat gagtcaagaa acttcattta tctatgagtt
      301 aagcaagtca aatttgaaat atacttataa tgaagatgaa cttgaaattt tagataatcc
      361 agaaaattca caatggatta aagagttgaa gagagaagac tttattgaga cactaagaat
      421 tctaaaatcg ttatttgatt tgaaattagc caaagctacc atcatgggaa ttgaaagaga
      481 ttcatattat tgggaggaaa ttggactata tgctccccag attgaaggtg aagataaaga
      541 gaaaaggata gacaacatta ttaactatat taatagttta agtgaacctt taaaatgtag
      601 aggacttgat tttataggaa cattaggatg gacagaaaca tactatgaga aaatatataa
      661 agaaataatt gaacaatatt ttcctaaata atttaataag gattgattta tcgtaaaaga
      721 aaaaagttgt gatttccaaa atcacaactt ttttcttttg tcaagatttt gagtgggata
      781 tatttttgta gaactgaaga tcatgaaatt atgagaggtt ggaggtaatg tagtcataca
      841 aataaaaatt ttttagaaag tgagtggtaa ttaatgaaaa tgaatcacac ttcaattcag
      901 aagcatttac aactgttaaa tacactgggg atttcttatg ttaatttgag aactttttct
      961 acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca agcaattatt
     1021 aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta taatcctatt
     1081 aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat tgaaaaaatt
     1141 cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac agatagcgaa
     1201 aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga aaagagaata
     1261 gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc cataaatgaa
     1321 caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt acttcaccga
     1381 agatttggag tagaagatga agttgatatt gatacttctg tttataatcc ttcaagatta
     1441 tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg tccgcatcgt
     1501 ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt taaaattctt
     1561 gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag ccatccatct
     1621 tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc tcactatcct
     1681 gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact tatatttgac
     1741 gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc tgttacggct
     1801 gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga tattcaagac
     1861 tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc attagaatcc
     1921 aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt tttggtgaaa
     1981 gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg caaaaatatc
     2041 gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg ggatgaaata
     2101 gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa acatggctta
     2161 atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca acgggagaat
     2221 gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga agcaatgatt
     2281 tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa aaactcatgc
     2341 taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca taagtatgtt
     2401 ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt gttaggctat
     2461 ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca gggcaagagc
     2521 acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa tccaactacg
     2581 gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc aaacaatcag
     2641 ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa ctttagctcg
     2701 ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa ttgtcagctt
     2761 atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg tttggaaaat
     2821 tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc tgatatcaag
     2881 tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa tacttttggt
     2941 tggggtataa agttatttct tttgaaactc tttgaacaag ggattacaaa aattgaagca
     3001 acttttgtcg aagctcaaaa aaaactatgg gctgttttac ctgaacattc tttgaagcaa
     3061 cgcattggta ataatctagc tttgatttat agtggagccc tcctaattca agatatactg
     3121 ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta cgaacagtta
     3181 attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa aacattgttg
     3241 gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc cagtacgatt
     3301 tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt tcctatcccg
     3361 tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct ttttaggcaa
     3421 ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg tattcacatg
     3481 aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa atcgtatttt
     3541 cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat ggaaatattt
     3601 cttattgatt tagcaaaaca actgattact tatctgtatg atagcgagca aacccaaacg
     3661 gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca tgaaagaaag
     3721 gaaaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc tgtatattca
     3781 agaacatttt tcaaatcaag ccttttttac aaaaccaatt tacttggatt tcgaaataag
     3841 aggggtatct gctggatcta tcgggggtac tcttcaagcg ttgaagaacg aaggatattt
     3901 agaaaatcac tttgtgcaac gttcgttcaa tagaagagtc gtgaaaaaat ggtatttagt
     3961 tcattcatag agaggggaat caagcatgcg tgtggcttgt tatgatcgaa cgtctgttta
     4021 tgaagaagcg aaaattagta atgacgctca atatgatgca gtgaaagagt atgttttaga
     4081 gaaatatgaa caaattcccc aacgattttc agacaaagga gaatcagggg aaaaagcgga
     4141 gcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag agggggtgat
     4201 ctatgtttgg cgttatgatc gattggcacg tcaagccaaa atagccttag aatttattga
     4261 gttgtgccaa caacatcagg ttcaagtgta ttcgatttct gaacctcttc ctgcttcttt
     4321 ctcacacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg cagaacaaca
     4381 acgaaacgtc attattgaga atgttcgtgc cggactttcc tataaaaagc aacaacggca
     4441 atatttgtct tctgcggtac catatgggta tcgtttagtg aatgggaaaa tccaacaaga
     4501 agtacacgaa gcaaggattg ttcgctatat ttttcaactt tatttgacca aaaaatatgg
     4561 ctacaaaaag ctttgtcaac gattgacgca acaaaagctt ttcttccgcg aacgtccttt
     4621 tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg aaatcaaagg
     4681 aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaag caaccttttt
     4741 acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt atctgtactt
     4801 attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga agtatcaatg
     4861 gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa gctgccaagg
     4921 aatttttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac aggctttttt
     4981 tcaatacgaa ggcgtctatc aagccttgat tgcccaatta acgcaacaat tagccgaaaa
     5041 gcgaaaagat gaaaaacgca aagtaatcca acaaaagaaa caagaagcac aatggattaa
     5101 ggcatttgaa gagggcaatt gttcactgga agaactcaag caacaactgc aagctcttca
     5161 aaagccgatg tctacttctt cgtttctttc actccaacag tgtgaagcaa aagtgaaaga
     5221 atggcttcaa ctaaaagacc tctctagtcg acaattatta tgtgactatg tttgtcggat
     5281 tgaggtaaca aaggataaaa cagtttgtgg gatttacttc cgacaactga cacaaaatat
     5341 tttagaaagg aacgaatgat ctatggaaat gatacaaacc aaacgagtcg cattgtatat
     5401 tcgcgtgagt acacaagaac aagctttaga aggacatagt attcaagcac aagaacaaaa
     5461 tggacgacag tttgccgaac ggatgggcta tgaagtggta gaagtttatg ccgatgaagg
     5521 aatcagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga acgatgctcg
     5581 gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac gcaatatgtt
     5641 agatgtctta caaacagtcg aggaattatt gcgatatgac gtaggattac attcgatctc
     5701 ggaacaattt gacgtgacca cttcgacagg taaattgatg ttacaactcc ttgggagttt
     5761 cggtgaattt gaacgcaacc aaattgcgga gaatgttcaa atgtcaatgc aaagcttagt
     5821 gaaagataaa aagcgttatg caggaggccg acgtttaggc tatgtcagtg gtattgacac
     5881 gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc gtctgatcta
     5941 tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga atcgtcaagg
     6001 atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata tcttacgcaa
     6061 taaaatttat gcagggtatt tagaatatgc gcgctacatc aattgggaaa ccaaacgacg
     6121 gaaggggaag aatccgagac cgattttagt gaaaggaacc catgaaccga ttatttcgga
     6181 agaagattat caaaaagtac aagaacgcct tgctttagaa ggaaaacaac ccaaatggaa
     6241 ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg gcgcaccgat
     6301 ggcagcgagt aatgtcacaa ataccctaaa ggatggcaca aagaaacgga ttcgatatta
     6361 ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt cgattcgtgc
     6421 cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc taccagaagt
     6481 tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc aaccattgga
     6541 acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa ttgaaaaatg
     6601 ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc gcttagacga
     6661 attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag ggatctttca
     6721 acaaggagag ccgattcaag tcaaagatgt ccaacggata ttgactaatt tggatcgttt
     6781 tcttgcccag agtgagaaga agcaaatcaa agccttgtat cgtaccttta tagagaaaat
     6841 aacctttgat cctaaccata aggagatgtt ggaaattacg atgaaattca cgcatgccgt
     6901 ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc aaaacaaagg atacggctat
     6961 ttttgtgtta aaaacaccct ttacgttaac gatctagtct aggttatatc aaacaacgtt
     7021 agaggggcgc taacgcttct ctagcgattt gaaatcgtta gagaagtagt tagggaggtc
     7081 cttatcataa atagggatat ttattaatga atatttgaaa tataattgta tataagaatt
     7141 aaaaagagtg gggatagata tgaaaaagat gggtaagtgt attttatgtg aaaaatacac
     7201 tgaacttact aaagaacatg taccacctaa aaaatcaggg aatactggag gtaaaaagac
     7261 cagaactggt aatttagatg atttccttaa gagcgatttt acaaaaggtg attttccgaa
     7321 ggggattaaa aggaaacctc aaggaaatgt ttattata
//
LOCUS       DARDHU010000114         7046 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000114 DARDHU010000000
VERSION     DARDHU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.115"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..1248
                     /locus_tag="SDD03_002456"
     CDS             94..1248
                     /locus_tag="SDD03_002456"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG4304879.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="SDD03_002457"
     CDS             complement(1270..2022)
                     /locus_tag="SDD03_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4304880.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="SDD03_002458"
     CDS             complement(2386..5052)
                     /locus_tag="SDD03_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4304881.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="SDD03_002459"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="SDD03_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG4304882.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2073 a         1455 c         1277 g         2241 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttc
//
LOCUS       DARDHU010000115         6936 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000115 DARDHU010000000
VERSION     DARDHU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.116"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..1151)
                     /locus_tag="SDD03_002460"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="SDD03_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="SDD03_002461"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="SDD03_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG4304883.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="SDD03_002462"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="SDD03_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2435..2665)
                     /locus_tag="SDD03_002463"
     CDS             complement(2435..2665)
                     /locus_tag="SDD03_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEG4304884.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2895..3713)
                     /locus_tag="SDD03_002464"
     CDS             complement(2895..3713)
                     /locus_tag="SDD03_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4304885.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="SDD03_002465"
     CDS             3874..4563
                     /locus_tag="SDD03_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG4304886.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="SDD03_002466"
     CDS             4577..6079
                     /locus_tag="SDD03_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4304887.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="SDD03_002467"
     CDS             6092..6568
                     /locus_tag="SDD03_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4304888.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="SDD03_002468"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="SDD03_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2252 a         1092 c         1265 g         2327 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatgt
//
LOCUS       DARDHU010000116         6888 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000116 DARDHU010000000
VERSION     DARDHU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.117"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..182)
                     /locus_tag="SDD03_002469"
     CDS             complement(<1..182)
                     /locus_tag="SDD03_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4304889.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="SDD03_002470"
     CDS             517..951
                     /locus_tag="SDD03_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4304890.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="SDD03_002471"
     CDS             974..1294
                     /locus_tag="SDD03_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304891.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="SDD03_002472"
     CDS             1435..1626
                     /locus_tag="SDD03_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304892.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="SDD03_002473"
     CDS             complement(1697..1954)
                     /locus_tag="SDD03_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG4304893.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="SDD03_002474"
     CDS             2112..2375
                     /locus_tag="SDD03_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304894.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="SDD03_002475"
     CDS             2399..2587
                     /locus_tag="SDD03_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304895.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="SDD03_002476"
     CDS             complement(2670..2855)
                     /locus_tag="SDD03_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304896.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="SDD03_002477"
     CDS             3070..3441
                     /locus_tag="SDD03_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4304897.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="SDD03_002478"
     tRNA            complement(3591..3663)
                     /locus_tag="SDD03_002478"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="SDD03_002479"
     CDS             complement(3709..4137)
                     /locus_tag="SDD03_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304898.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="SDD03_002480"
     CDS             complement(4197..4955)
                     /locus_tag="SDD03_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG4304899.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="SDD03_002481"
     CDS             5250..5447
                     /locus_tag="SDD03_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304900.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="SDD03_002482"
     CDS             5553..6035
                     /locus_tag="SDD03_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304901.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="SDD03_002483"
     CDS             complement(6093..6689)
                     /locus_tag="SDD03_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4304902.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1060 c         1354 g         2143 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgc
//
LOCUS       DARDHU010000117         6570 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000117 DARDHU010000000
VERSION     DARDHU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.118"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SDD03_002484"
     CDS             127..579
                     /locus_tag="SDD03_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304903.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SDD03_002485"
     CDS             593..1282
                     /locus_tag="SDD03_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304904.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="SDD03_002486"
     CDS             1407..3758
                     /locus_tag="SDD03_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG4304905.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="SDD03_002487"
     CDS             3838..4233
                     /locus_tag="SDD03_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304906.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="SDD03_002488"
     CDS             4558..5175
                     /locus_tag="SDD03_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4304907.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="SDD03_002489"
     CDS             5216..5515
                     /locus_tag="SDD03_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG4304908.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="SDD03_002490"
     CDS             5549..5716
                     /locus_tag="SDD03_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4304909.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="SDD03_002491"
     CDS             complement(5761..6366)
                     /locus_tag="SDD03_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4304910.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DARDHU010000118         6460 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000118 DARDHU010000000
VERSION     DARDHU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.119"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..3055
                     /locus_tag="SDD03_002492"
     CDS             <1..3055
                     /locus_tag="SDD03_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEG4304911.1"
                     /translation="FLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLADNEDLATTV
                     LSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVKNLLGNNIDL
                     LAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNIFPSDKTAVG
                     HDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHPGGAAMVNDQ
                     KGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADGIGNITTVS
                     STLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDSLSEKSDQYL
                     GIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGATWNKNLLNGW
                     NSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGSFIARVNQL
                     GNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVGGIGKGVNG
                     VISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEEYVQMPDGR
                     SFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLLDDEKRMLE
                     FLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGAVNANGVYQ
                     YLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYTAAANYAFE
                     KFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMGSGDIFNGGV
                     GGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGNDGLAEGNAT
                     GLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGKGHGYANGG
                     LITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKSEINSVDNRH
                     ILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            3059..3931
                     /locus_tag="SDD03_002493"
     CDS             3059..3931
                     /locus_tag="SDD03_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG4304912.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            3928..4977
                     /locus_tag="SDD03_002494"
     CDS             3928..4977
                     /locus_tag="SDD03_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304913.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            4981..5805
                     /locus_tag="SDD03_002495"
     CDS             4981..5805
                     /locus_tag="SDD03_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304914.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            5823..>6460
                     /locus_tag="SDD03_002496"
     CDS             5823..>6460
                     /locus_tag="SDD03_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG4304915.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         2304 a          990 c         1295 g         1871 t
ORIGIN      
        1 gtttctttcg gctaataata ctaaatttac agaggttctt ttatcatctg acagaatgtg
       61 gaatcaatgg gtaaaccttc ctgataatga gaaaagatta cttgcagata acgaagattt
      121 agctaccact gttttatctt cagaagaaat atacggaaga tggactcaat taccagataa
      181 tcaaaaatat atgttagctg acaatactga cttagcaaac aaaatattcg cttcagaaga
      241 atcatggaat gcatggaaag caattccaga cacagttaaa aacttgcttg gtaataatat
      301 tgatttactg gctaaagtga aagacgggac tgtaagtgta gaagaatata acaaagttat
      361 gccacttctt aaaactctat atggagataa cgttaattta aaagaggcaa tcagtggggc
      421 taaaagctct atagagaatt acaacaaaaa tatttttcct agtgataaaa ctgcagtagg
      481 acacgatgag gcttcacaag ctgctaaaga tgcatttgaa gcgttcaaca tcttccaaac
      541 aaaaatacca gataaaatta ctaaaactgt atcagctgac tttataggtc ctatgccgaa
      601 aaatgcgaaa ggaacaaact tccatcctgg cggagcagct atggtaaacg atcaaaaagg
      661 gcctacttat gaagagctta taaccttgcc aagcggagaa acttttatac ctaaaggacg
      721 taatgtcgtt ttagacttac caagaggttc gaaagtactg aacgccacta aaactaaacg
      781 tctagtgcct aaatatgctg atggtatagg aaacataacg acggtttcat ctacgctaaa
      841 cttagatgct ttaattttag ctattaacga gctaacgacg gtattgagaa ctcaactgat
      901 ggcaggaacg actgatgcta aagatactaa actatcagat gaaacaaaag ttgccgatcc
      961 gattattccg gacagtcttt ctgaaaaatc agatcagtat ctaggaattg gagctcaatg
     1021 gttaactaat ctaatgaatg gttggaactc agcggttcct caatatatga atagtgaaat
     1081 ggtttttatt acaaactatc tcaatgcgct aaaacttcaa aacaatccta attatacgca
     1141 aggagcaaca tggaataaga acttactgaa tggatggaat agtttaactg ggacctttat
     1201 tgctacaatc aattcatttt gtaatcaagc aatggtcacg cttagaaact acaacactcc
     1261 tatgtacaac aatggccgaa cgtggcagca aaataatctt aatggttgga attcattgta
     1321 tggatcattc atagcccgtg taaatcagct tggtaacgat tcgattaaca accttcgttc
     1381 taaaagtggt ggtttttaca atgcagggac attcttgttg caatctttaa taaacggaat
     1441 gaattcgtta ggtaattctc tttctactac aatgaacaat gtcgctaata caatggttgg
     1501 tggtataggc aaaggagtta atggcgtcat atcgggagtt aactatgtgc ttaaggaagt
     1561 cgaatcaagt aagaatatcg gaaactggcc aattccacaa tacgctaagg gaactgatgg
     1621 acatcctggt ggacttgcta tgattaatga tcaaaaaggc ctagttcacg aagaatatgt
     1681 tcagatgcca gatggacgta gttttattgc caaaggtaga gatcttcttg ttaatttgcc
     1741 taaaggtgct caggtcttaa atgcttcttt aacaaagaaa ttaaaggaac gtttaaatat
     1801 tccacactac gaaaaaggta ttggcaattt agatattgtt gatttgttag atgatgaaaa
     1861 aagaatgtta gaattcttaa ctagcaaagt tgatttttca ggcattaatg aaccatggct
     1921 tgatatgact aaatcaggaa cgtcattaat gtccaaagct gcaaatacaa tgcttcaatc
     1981 aaaattaagt gaattcttca ctcatggcaa ttttgatggt gcagtaaatg ccaatggtgt
     2041 ttatcaatat ttagttgatg tggcacagaa agtaatgggt aagttccccg gacttacagt
     2101 aacttctgga tatcgagcag gagatgccta ctatcacgga aaacggcaag ctatcgactt
     2161 ggcttatcca ggtatttctg gagatccgag atatacagca gcagcaaatt acgcttttga
     2221 aaaattccct tcaaaaattg cgtatgtcat tacgaatgga cgtgtacgtg accgcatggg
     2281 attatctgga acaaggtcaa gcggacaatg gacgaattgg ccagatggcg atcacttcga
     2341 tcatattcat ttaaacggtt cgatggggtc tggcgatata ttcaatggag gagttggagg
     2401 aagtggtgtt gcacgctggc gttcttatgt gtctaaagca ttgaaaatga atggattacc
     2461 agctacagca gcgtatataa atgcctggat gtcccaaatc caaacggaat ctggtggtaa
     2521 tgagaaagca atcggcggca atgatgggct cgccgaaggc aatgcaacag gattacttca
     2581 aacgaaaccc ggaacttttg cagctaacgc ttttcctggt cacggaaata ttatgaacgg
     2641 atttgataac atgttagcag caatcaatta tgctaagaaa cgttacggag ttgcaggtat
     2701 gttgcaagtt atcgggaaag gacatggata tgccaatggc ggcttgatta ccaaagacgg
     2761 actatatcga gcaggagaag gaaataaacc agaaatggtt attccattga caagaaaaac
     2821 aagagctatc gaacttatgg gtcaagcatt agccttcctt tctggaagca ataaaaaaga
     2881 aacgaaaagt gagattaatt ctgtagataa tagacatatc ctacaagcga tagaaaaaca
     2941 gtctcgtatg acaaatgatc tgctatcatt attactctcg ttttttaaaa gtaacaatcg
     3001 atcagataaa gaactagctt tagatattca gaaaatttta atcaggagga catagtattt
     3061 gaggacagta atattaagaa ataaattgaa tgaagaaatt gatttgtcca cagaagatta
     3121 ttttgctact gaaatgtcga atctaggttt tgaagttaca aaagagcata ttggacaatg
     3181 ggggagtttc agagaaatag gagagagtat tgaattgtct gaatttcaat catcagttat
     3241 gatttctgtt cacggattta gagaacaaca attatatggt tcattagttg aatttctggc
     3301 taatggacct tttgaactag aattttcttt tgattcagaa acaatgatca ggaaatgtag
     3361 tttgaaatca ttatctaaaa cagaaattga ttcccaaagc tcattgctaa cagatacttt
     3421 gaatctatat tttacttcaa gctggtattc tattaagcgt gaaaaattaa tacagaattc
     3481 aacttctctc aatacacgtg gaaaagttta ctcatttgtt cgaccatatg tctacaccaa
     3541 aaatgtatgg gaaaagaaag gtgtatttaa gttcagtaat aattctgtat atttgacaaa
     3601 tagtacagag cgaatgtctc cgttaaaaat aaggattatt ggagagtgct caaatccata
     3661 ttgggaagtt atccaaaact ctaaagtagt agcttcagat ggctatttta ttaatatgaa
     3721 agacacacaa atattagaag tgtctagttt gctagaagac caaacggcaa ttttaaaaga
     3781 tgtaggtgga gttgaatctt ctgtttatca acaacaagat tttactaaat caaacttcgt
     3841 ccaagcgcct ctaggagaat tcagcgttgt ttttcatgtt ggacatgcaa atgtagagat
     3901 tgaactttat gaggagcgtg atctattttg attttagcca ttaccctctt tcaccgagat
     3961 atgaatttat ataacgaacg tttaatttcg actgagttcg aatttggaac ggatgaaatt
     4021 ggagaagaag taagtaattt tattacagat aaatatgtcc atgtaaaatc aggagatttt
     4081 ctactagcaa aatttattcc tagcgggaag attgcttatt ttggagtgat tagctcgcaa
     4141 gaagacaaaa aaataaattg caaaggatta ctcagtttgg ccgatagcga gataccgact
     4201 gtaaaaatat caggtgctaa ttatgaagag catattcgac gactgattga atactactta
     4261 ttgaacgatc caacaaaaca actaaaaaat attttggagg taaaaacaga aagcgttact
     4321 tctcattctt atcaagctgt tgacacaaat aagagacgat tgaatgcata tataataaat
     4381 gggttcaaaa aatataatgt taaatggtat gtcaaagaga ttaaaggtaa aaaaatatat
     4441 actggtattc gagcagttga tgattcaatt tatattaaag ataattcatc tgagtttaat
     4501 gactgggatg tttttgtgca atctcctgga ccagggaacg agaacaaatt attgatcgta
     4561 gataaagcaa tgaaagatat agaaaatccc ttggtattgt ctacgtggta tttagatgac
     4621 gaaaataatc ttacgcaaga ttcaacgaat aagaacattg cattgcctac cgttaattta
     4681 gtaaatatat atgatcaaac tcaagaagat agaccttctt atgaagaggt agctaaatcg
     4741 gagcttaaag gcaacgccta ttcacatgaa attaaagtta atgttgtgct aaatgctaga
     4801 aatatcaatg tagaaaatat cgaaacagga atgttagcga ctattgttta caaagagcag
     4861 acttacaaat ctgttctaac tgcctggcga atatccagca gtaaaaagaa tatagaattg
     4921 acatttggta atgtccgaag tcgttttatg gattattttg aagataatgg gggataaaaa
     4981 atggttagca atgtggatgg atatcaattt gaaaacgtga aagtaagcgc agaaaatgat
     5041 gctagacttt atcacgtttt atataatcgg aaaaatcagg ttattgatgg ttacgatcag
     5101 tctatgaatt tatcttcaag cggattaaca gtaaaagttg ctgcaggagc agcgattatt
     5161 caaggtcgta tggtcgttgt tcgacaagaa gaaagtataa cagttccagc aaactcaagt
     5221 ggttatatag cattaacagt ggatttgaca caagaagtta tacctggatc tattcttcca
     5281 gaatcggaag aatatgaatg gactaataat caagtcaagc tagaatttat aacaaaagtt
     5341 ataaaaggta atttaaacaa tggtgataag gtctataatt taccactatg ctcagttaat
     5401 tctactggat caactgtttc aatctcaaag atatcggata gttacgagct gactctctct
     5461 aaaggagaaa ttttgtggag ggggactgcg ttaatgcatg atactcaaac tgtccaacct
     5521 tcaaaaaaaa tttggcagac agtttcaggt tttttgttag tatggctccc atacgaaaac
     5581 gggcaagcaa ttgaggatag atatgtaact acgccttttt ataaggagcg tataactttt
     5641 actaatgttt taggagaaat tgtttcagga tttgatgact atcacaaaaa gtggtttagt
     5701 aaacgaataa actataattc gaatactaat atatttacag gtgcagcaag caatgcaagt
     5761 ggagataacg caaatatggt gcttaggtat atagtttctt tttagttagg aggtatggaa
     5821 gagtggcaaa tttagaaatt aaattatctg caaataagag acagccttat ctacgtcacc
     5881 gtgttgttgg tagagttggt gatggggggc tcacaacaat caatgtacaa cttcttgagg
     5941 aagatgaaat tacacctttt gtaattaatc taaacggtac tttgaaattt gtgggcgaag
     6001 tttcaaacgg taactatacc gagggagaac cagaaataat cgattcgact aatgggttaa
     6061 ttagttacac gttcactaag tcaaatttca gcacgagtca tcaattcaaa caagcatatt
     6121 ttgaatatgt agatcctaac ggcaaaaaag taacttttca gaacttcatc atagacgtgt
     6181 tagaacgagt agatattaat tcggagcaag cgaaatacta tatttcttca ttggaaaaat
     6241 tacagagtga aatgcaaacc actttcaatc agttcattag tgataaacag gtccaatacg
     6301 atcaaatcta ctcgaaatat aacgaattag taagattgat aaatgaatca gataagcaag
     6361 taaatgatcg tattgataag gcaaataatc gtattgatca aaccaatcag caaatcggcg
     6421 atctcggcaa gctgaaaaag atgtacagta acagcattga
//
LOCUS       DARDHU010000119         6285 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000119 DARDHU010000000
VERSION     DARDHU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.120"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SDD03_002497"
     CDS             <1..177
                     /locus_tag="SDD03_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304916.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="SDD03_002498"
     CDS             190..690
                     /locus_tag="SDD03_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4304917.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="SDD03_002499"
     CDS             787..2634
                     /locus_tag="SDD03_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG4304918.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="SDD03_002500"
     CDS             2637..3533
                     /locus_tag="SDD03_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4304919.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="SDD03_002501"
     CDS             3583..3972
                     /locus_tag="SDD03_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4304920.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6285
                     /locus_tag="SDD03_002502"
     CDS             3959..>6285
                     /locus_tag="SDD03_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4304921.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLE"
BASE COUNT         2118 a          932 c         1347 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaa
//
LOCUS       DARDHU010000120         6100 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000120 DARDHU010000000
VERSION     DARDHU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.121"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(312..797)
                     /locus_tag="SDD03_002503"
     CDS             complement(312..797)
                     /locus_tag="SDD03_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304922.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(936..1151)
                     /locus_tag="SDD03_002504"
     CDS             complement(936..1151)
                     /locus_tag="SDD03_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG4304923.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1375..2175
                     /locus_tag="SDD03_002505"
     CDS             1375..2175
                     /locus_tag="SDD03_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4304924.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2194..4062
                     /locus_tag="SDD03_002506"
     CDS             2194..4062
                     /locus_tag="SDD03_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG4304925.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4055..4546
                     /locus_tag="SDD03_002507"
     CDS             4055..4546
                     /locus_tag="SDD03_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG4304926.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4534..5088
                     /gene="srlA"
                     /locus_tag="SDD03_002508"
     CDS             4534..5088
                     /gene="srlA"
                     /locus_tag="SDD03_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG4304927.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5106..>6100
                     /locus_tag="SDD03_002509"
     CDS             5106..>6100
                     /locus_tag="SDD03_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG4304928.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2157 a          968 c         1221 g         1754 t
ORIGIN      
        1 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
       61 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
      121 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
      181 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
      241 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
      301 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
      361 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
      421 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
      481 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
      541 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
      601 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
      661 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
      721 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
      781 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
      841 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
      901 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
      961 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     1021 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     1081 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     1141 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     1201 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     1261 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     1321 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     1381 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     1441 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     1501 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     1561 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     1621 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     1681 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     1741 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     1801 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     1861 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     1921 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     1981 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     2041 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     2101 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     2161 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     2221 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     2281 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     2341 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     2401 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     2461 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     2521 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     2581 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     2641 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     2701 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     2761 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     2821 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     2881 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     2941 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     3001 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     3061 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     3121 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     3181 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     3241 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     3301 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     3361 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     3421 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     3481 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     3541 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     3601 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     3661 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     3721 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     3781 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     3841 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     3901 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     3961 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     4021 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     4081 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     4141 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     4201 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     4261 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     4321 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     4381 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     4441 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     4501 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     4561 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     4621 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     4681 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     4741 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     4801 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     4861 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     4921 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     4981 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     5041 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     5101 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     5161 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     5221 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     5281 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     5341 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     5401 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     5461 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     5521 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     5581 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     5641 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     5701 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     5761 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     5821 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     5881 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     5941 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     6001 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     6061 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DARDHU010000121         6002 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000121 DARDHU010000000
VERSION     DARDHU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.122"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SDD03_002510"
     CDS             <1..395
                     /locus_tag="SDD03_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304929.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SDD03_002511"
     CDS             398..1228
                     /locus_tag="SDD03_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG4304930.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SDD03_002512"
     CDS             1244..2095
                     /locus_tag="SDD03_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4304931.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SDD03_002513"
     CDS             2092..2451
                     /locus_tag="SDD03_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG4304932.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SDD03_002514"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SDD03_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SDD03_002515"
     CDS             2624..2929
                     /locus_tag="SDD03_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304933.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SDD03_002516"
     CDS             2929..3240
                     /locus_tag="SDD03_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG4304934.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SDD03_002517"
     CDS             3237..3410
                     /locus_tag="SDD03_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304935.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SDD03_002518"
     CDS             3407..3823
                     /locus_tag="SDD03_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG4304936.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SDD03_002519"
     CDS             3823..4491
                     /locus_tag="SDD03_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG4304937.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SDD03_002520"
     CDS             4488..4865
                     /locus_tag="SDD03_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304938.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SDD03_002521"
     CDS             4862..5161
                     /locus_tag="SDD03_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304939.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SDD03_002522"
     CDS             5151..5309
                     /locus_tag="SDD03_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304940.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SDD03_002523"
     CDS             5403..5819
                     /locus_tag="SDD03_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG4304941.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DARDHU010000122         5940 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000122 DARDHU010000000
VERSION     DARDHU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.123"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(166..624)
                     /locus_tag="SDD03_002524"
     CDS             complement(166..624)
                     /locus_tag="SDD03_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4304942.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(637..1968)
                     /locus_tag="SDD03_002525"
     CDS             complement(637..1968)
                     /locus_tag="SDD03_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG4304943.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1979..3421)
                     /locus_tag="SDD03_002526"
     CDS             complement(1979..3421)
                     /locus_tag="SDD03_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4304944.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3408..4172)
                     /locus_tag="SDD03_002527"
     CDS             complement(3408..4172)
                     /locus_tag="SDD03_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG4304945.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4276..5151)
                     /locus_tag="SDD03_002528"
     CDS             complement(4276..5151)
                     /locus_tag="SDD03_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4304946.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5406..5490)
                     /locus_tag="SDD03_002529"
     tRNA            complement(5406..5490)
                     /locus_tag="SDD03_002529"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5454..5456),aa:Leu,seq:caa)
     gene            complement(5508..5579)
                     /locus_tag="SDD03_002530"
     tRNA            complement(5508..5579)
                     /locus_tag="SDD03_002530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5545..5547),aa:Gln,seq:ttg)
     gene            complement(5589..5661)
                     /locus_tag="SDD03_002531"
     tRNA            complement(5589..5661)
                     /locus_tag="SDD03_002531"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5626..5628),aa:His,seq:gtg)
     gene            complement(5723..5807)
                     /locus_tag="SDD03_002532"
     tRNA            complement(5723..5807)
                     /locus_tag="SDD03_002532"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5771..5773),aa:Leu,seq:caa)
     gene            complement(5828..5898)
                     /locus_tag="SDD03_002533"
     tRNA            complement(5828..5898)
                     /locus_tag="SDD03_002533"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5864..5866),aa:Cys,seq:gca)
BASE COUNT         1824 a         1260 c          903 g         1953 t
ORIGIN      
        1 ttaagaatcc tgaaaataag ttttctaaat cataggagca acaaaaaaat tcgataatta
       61 acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta
      121 aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca
      181 actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg
      241 agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat
      301 agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact
      361 tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact
      421 ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc
      481 atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa
      541 gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat
      601 gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt
      661 tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt
      721 ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac
      781 tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg
      841 ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac
      901 tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg
      961 acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa
     1021 attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt
     1081 atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc
     1141 aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa
     1201 atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt
     1261 aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc
     1321 tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata
     1381 cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga
     1441 aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc
     1501 agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg
     1561 tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc
     1621 gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc
     1681 tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc
     1741 aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat
     1801 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
     1861 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
     1921 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
     1981 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
     2041 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
     2101 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
     2161 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
     2221 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
     2281 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
     2341 cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
     2401 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
     2461 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
     2521 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
     2581 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
     2641 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata
     2701 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
     2761 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
     2821 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc
     2881 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
     2941 agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
     3001 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
     3061 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
     3121 taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
     3181 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
     3241 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg
     3301 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
     3361 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
     3421 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
     3481 aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga
     3541 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
     3601 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
     3661 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
     3721 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt
     3781 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
     3841 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
     3901 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
     3961 ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
     4021 tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat
     4081 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
     4141 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
     4201 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
     4261 agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt
     4321 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
     4381 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
     4441 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
     4501 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
     4561 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
     4621 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
     4681 gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga atctcatttc
     4741 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
     4801 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
     4861 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
     4921 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
     4981 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
     5041 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
     5101 taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt
     5161 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
     5221 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
     5281 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
     5341 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
     5401 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
     5461 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
     5521 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     5581 gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
     5641 taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact
     5701 aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg
     5761 gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt
     5821 agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta
     5881 ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa taaattatag
//
LOCUS       DARDHU010000123         5924 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000123 DARDHU010000000
VERSION     DARDHU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.124"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(75..1169)
                     /locus_tag="SDD03_002534"
     CDS             complement(75..1169)
                     /locus_tag="SDD03_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG4304947.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="SDD03_002535"
     CDS             1358..2119
                     /locus_tag="SDD03_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4304948.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="SDD03_002536"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="SDD03_002536"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG4304949.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="SDD03_002537"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="SDD03_002537"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG4304950.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4772)
                     /locus_tag="SDD03_002538"
     CDS             complement(3759..4772)
                     /locus_tag="SDD03_002538"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG4304951.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4937..5857
                     /locus_tag="SDD03_002539"
     CDS             4937..5857
                     /locus_tag="SDD03_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG4304952.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1180 c         1063 g         1875 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DARDHU010000124         5831 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000124 DARDHU010000000
VERSION     DARDHU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.125"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(130..216)
                     /locus_tag="SDD03_002540"
     CDS             complement(130..216)
                     /locus_tag="SDD03_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG4304953.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            892..1842
                     /locus_tag="SDD03_002541"
     CDS             892..1842
                     /locus_tag="SDD03_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG4304954.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1863..2396
                     /locus_tag="SDD03_002542"
     CDS             1863..2396
                     /locus_tag="SDD03_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4304955.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            2412..2582
                     /locus_tag="SDD03_002543"
     CDS             2412..2582
                     /locus_tag="SDD03_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304956.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            3032..3565
                     /locus_tag="SDD03_002544"
     CDS             3032..3565
                     /locus_tag="SDD03_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304957.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            3577..3780
                     /locus_tag="SDD03_002545"
     CDS             3577..3780
                     /locus_tag="SDD03_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304958.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            4102..4482
                     /locus_tag="SDD03_002546"
     CDS             4102..4482
                     /locus_tag="SDD03_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG4304959.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            4464..5378
                     /locus_tag="SDD03_002547"
     CDS             4464..5378
                     /locus_tag="SDD03_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG4304960.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            5338..5616
                     /locus_tag="SDD03_002548"
     CDS             5338..5616
                     /locus_tag="SDD03_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304961.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            5623..>5831
                     /locus_tag="SDD03_002549"
     CDS             5623..>5831
                     /locus_tag="SDD03_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304962.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSR"
BASE COUNT         2112 a         1027 c         1089 g         1603 t
ORIGIN      
        1 caaaattgaa tttgattagc aatcatgtta aactgaacct gcaaatgatc aaacattaga
       61 tacacgaaag ccccagagag gtcggattct ctggggcttt ttttcaggtt gatgtcgcct
      121 ttattgattt tacttcttgt ttcggttatt caaccaataa ctgaataaag agacacaaac
      181 ttctacaaga agggggcaaa tgaaccgttc aaacattaga tacacctcct tccagtcgca
      241 agactgtcgg ataggcgaca agttattata ccttacaaaa taaaatctgt gtggtgtttt
      301 taagtatccc ttattttcgg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa
      361 gaacaagata aggtttttta cccaagactg tcttaaaacg acttagacga gctatcagag
      421 gcttccccca cctccgttac tgacttgttt ttagcaaagg agcctttagg cgactgcgca
      481 gtacaatgtg agagcatagc gatcacactt ttttagcgac cgtagggagc aaggttttga
      541 attggcagtt ttgaaaaatg cgcgaacctg ttggtagaaa aaagaagtgg tttttaggcg
      601 ggaaattttg tcaccctctt ttataatctc tttttaaacc tttttttaaa cctctttacc
      661 gtcggctgtg tccttactcc ttcaatggat acagcgtttt ataaactaca ttttgtctgc
      721 ttttaaacta cattttgtct gcttttaaac tacattttgt ctgcttttaa actacatttt
      781 gtctgctttt aaactctttt tttgcactca cgtatgttat aatatgcatt atcacagaca
      841 aaacgttgtt tataacgtct gtatccattg aaggagtaag gaggaatatc tttggcaaat
      901 gaaatcgtga aataccatca tgaattaaat accattccgt tgcggaaatt tacaccagtt
      961 gaaatgaatc tatttttctc tattgtgtct agaatgcgcg aaaaaggcga taaaacagtc
     1021 cgtcttactt ttgaccaact aaaagattta agtaattata aagcaactgc taataatcgt
     1081 ttcattaatg atttagaaat gacttatgat aaattaatgg atttacgttt tggtaaaaga
     1141 agtgctaatg ggctcgatat agatagattc gttatgttta gtgagtttaa aatacgtgga
     1201 actgctaaag atccatatgt agacatacaa gtttacgaaa aagctttacc tttactaaac
     1261 aatttagatg agtgggttcg ctattcactc caacaattta acgaattaca aagtagctat
     1321 gcaaagacta tgtttcgatt actaaaacaa tatcgaacta aaggttttgc ttatttttct
     1381 aaagaggatt ttcatgaact attagatatt ccaaaaagtt actggaacaa ccaagcaaac
     1441 gttgataatc gtgttataaa gcctatacga caggaattaa ccgcaatctt caaaggatta
     1501 tcaatcaaaa agaaatacgg aaaagggcga ggaaaacctg tcgttggcta tcaatttacg
     1561 tttaaaccag aaatcaaaaa agcagatgat tttaataaac atgcaccaac caaagaagaa
     1621 caaaataaag cattggcttt agctgataaa caaaaagaat acgaagaacc tgctacaaca
     1681 attaacgaaa aaatcaaaca gaagaaacga gaacttgaac tcctccaaca aatcaaacaa
     1741 ctagaaaaag agaaagaagc tcttgaaaaa caaacagagt ccgcactttc gaaaaaagag
     1801 aaaaaaagtt tattatctga tttagataat ttattccagt agcaacctag gaggcaacca
     1861 gtatgcaacc acaagaaacc tttacaataa aggaattgtc agaacttttt aagatgagta
     1921 ggcaagcaat atcgaaacat atccaaaaat tagattcttc catgatagct aaaaatgagc
     1981 gtggttataa ggttgtattg cgttctggag tattgcaact tgctcgcaac ttaggcaacc
     2041 aaatgttgct agataggttg cacgagtacg aacaagacaa cgataaaaat gttaatatac
     2101 caagcaaaaa cgtggttaca aacaagttgc tagatcagtt gcgtgaagaa aatgaatttt
     2161 tacgagaaca acttgccctc agagagcaaa ataattctca tatacaaaaa ctactcgacc
     2221 agcaacaaca actcacccta caagccaaca aacaaatcga aaaactgcaa gaaaaactac
     2281 agttgactta tacagaagaa gctccagaga atgactcaac gactctttct gaagcaacag
     2341 aaacaataga caaacaaatt gatactgaaa aaaaatggtg gcaactttgg aaataactgg
     2401 agggaacatt catgacaaga aaaaatcttc tattcggttc tattttacta ggaattctag
     2461 gattcagtcc gatgttattg tcagtgatca ataaagggaa taaacatggt gaattgaaat
     2521 tgaatccaaa ttattacaac agtcctaacg aagaattgtt tgtaaaagaa acagataaat
     2581 aataaaaaag actgctcttt cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa
     2641 aaagcaacgc gcttatgaag cgctaccaaa aatttatcat attttattaa aaataacaac
     2701 ccagtaaaaa atgactggta aaaattcaca aagaaacgga aagaagcaac cgcagaaaat
     2761 caagcttaaa aagagctttt gacctagacc aatcgaaaat cacaaagtga gcgcctgtat
     2821 ctattgctac aggcgctact tggcatagtg tacattatgt caacaagaat ttaatataac
     2881 atattttcct aatgcattct aactatacca cattatcaaa aatataaatt atctttacta
     2941 aataaaatat tgacaagcct ataccttttg ttttatgatt ttcacaaaga aaacgttttc
     3001 tatatatttt tctgtatcag gaggaaattc tatgaaaaaa tttactttag gggtagcttt
     3061 gttaactatt ggggcagttg ttttaccgtc aactactata gtttatgctt cagaaaattc
     3121 ccaagtggta tcattaaacg aaaacaatgc tactgaaaat gtagttattg agtacactga
     3181 agatggtatg gtcgtaacta aatatgagga tggttatagt gcggaagatt ttgcaaattc
     3241 ttcagatatc atgacaagag ctgcagttgc aagatggggc aaaaaggaat atacaaatat
     3301 tgctgtaacc actggagtag ctgctaatgc tattaatgct gccttttggg caggagggaa
     3361 agctagcact tctatgtttg gtatcccagg ttgggcaatt ggtgctttgt taacaggtgc
     3421 aagttggact aaaaaaggtg cttctccagg gaatgcagtt gcaaaagaat gggacaaaaa
     3481 tggtaatggc tggattggat tttattacca aaggggatat gatgcagctg gaagagttgt
     3541 agctactaga tattcaacgt tataagttaa ggagtgatgt ttttgatatc taaatcatta
     3601 aaatcattaa aaacatttac taaaaagaat ccgcaaatag ctactattcg tatttttata
     3661 tttgctatag ctgttgctat aattgttaat atgctaagcg gagggcaatt atacaaatct
     3721 ggtttatttt tagtgttaat cttagttgtc ttagacataa taaagtctaa aaaaaaataa
     3781 atcgatacat tttagaagaa tagggttgtt tgaatattac aaagcaacaa tttcaccttc
     3841 ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt ttttgtattg
     3901 tggagcgtgg attttgaggg ggattcaagg gggagcttgc gctcctttga caagcggtta
     3961 tttgcaccaa atacggaggt atttggctag caaatataga gcttgccaaa cccttttagt
     4021 gagctttact cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga
     4081 cgtactgaaa gggtgggact tatgagcgaa caacatcaaa atttggctag cgatcattct
     4141 aaaaaatata cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa
     4201 tttgcaaagc tgaagcagtc agctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa
     4261 gccaaggcac aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca
     4321 atcgcgcgcg atttagccaa agctggaggg aatatcaatc aaattgccaa gtggtgcaat
     4381 acacaccaac acgacgttgc tcctgatgat gctcaacgtt tatccgaaaa cctaaaaatc
     4441 atgcaaaagg agctacaaaa gatatggcaa cagttaaagt aagtcgaacc acttcatgta
     4501 gccgagcaat caattacgca gaaccacgag caaccgtcaa aacaggaata aactgtgaca
     4561 ttgattatgc caaaagtgaa atgaaacaaa tacgcacgct ttatggaaaa gacgatcgtg
     4621 tacaagcaca cttattgatt cagtcctttc gaccaggaga aattaccgct gaaaaagcca
     4681 atcaactagg ggcagagtat gcgaaaaaaa tagcccctaa ccaccaaatc gcgatttaca
     4741 cacatacaga caaagaccat attcataatc acattgtcat taactccgtt aatcttgaaa
     4801 ctgggaaaaa atttcaagcc catgggcaag catttttaaa caaatgttat gacctaaatg
     4861 acgagatttg tcttgaacat gacttaacaa tcacggaacg aggaaagaaa accgaaaaac
     4921 gaaccatgtc tgaaattaaa ttaaaagaaa aaaatgaacc ggtttggaaa gacgaaattc
     4981 ggtttgcgat tgatcaaaca atgaaaaaca aaaaaactcg aacgtatacc caattttgtg
     5041 attcacttaa aacattcggc attcattgct tgaatcgcgg aaaaaatttt acttacgaat
     5101 tggtccacaa aaagaaaaaa gtacgatcca acaaactagg aaaggattac gaaaaggaga
     5161 cgattttaca tgagctggac agacgagaac gaacctacaa tcaacaacga accaacgagc
     5221 tacgaagagc tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa
     5281 cttttacgcc tgaacaatcg gaaattagac cgactcgaag aaaccatgaa caacacgttg
     5341 aacgaggaac agaccaccaa caagaattat cgctctaaaa cgttaaaaga catcgaaaac
     5401 gaaataaaga gacaacgtgc ctttcttgcg ccttataccc aagaaaggat acaaactacg
     5461 atggattcac aagtcaaaaa actccaccaa gcgctattag accagcgaaa ccaagaacaa
     5521 aaaaaggctt taattcaacg atcgatggat attcttgccc ccttatgttg cttagtacta
     5581 acattattag ccttttggtt ggtatgggga gaataaacag acatggcatt ctatttaccc
     5641 tatctactca tttctgccag tattttagga attatttggc ttagctataa ggcttatcaa
     5701 acaagatatt cattaaaagg ctctaaaatt cgatttaagc gatttttatt ggtcggggtt
     5761 atatttgcct tatcaatcgt tttaagtggt ttattcggcg ttttagaggg taataaacgt
     5821 gtttcaagaa g
//
LOCUS       DARDHU010000125         5809 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000125 DARDHU010000000
VERSION     DARDHU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.126"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SDD03_002550"
     CDS             450..1604
                     /locus_tag="SDD03_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG4304963.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SDD03_002551"
     CDS             1598..2020
                     /locus_tag="SDD03_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304964.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SDD03_002552"
     CDS             3143..4075
                     /locus_tag="SDD03_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4304965.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SDD03_002553"
     CDS             4092..4607
                     /locus_tag="SDD03_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4304966.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SDD03_002554"
     CDS             4903..5184
                     /locus_tag="SDD03_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304967.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SDD03_002555"
     CDS             5186..5602
                     /locus_tag="SDD03_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304968.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARDHU010000126         5790 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000126 DARDHU010000000
VERSION     DARDHU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.127"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SDD03_002556"
     CDS             complement(99..1229)
                     /locus_tag="SDD03_002556"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG4304969.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SDD03_002557"
     CDS             complement(1877..3079)
                     /locus_tag="SDD03_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4304970.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SDD03_002558"
     CDS             complement(3139..3342)
                     /locus_tag="SDD03_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG4304971.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SDD03_002559"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SDD03_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SDD03_002560"
     CDS             4879..5691
                     /locus_tag="SDD03_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4304972.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DARDHU010000127         5731 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000127 DARDHU010000000
VERSION     DARDHU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.128"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            362..1873
                     /gene="gadC"
                     /locus_tag="SDD03_002561"
     CDS             362..1873
                     /gene="gadC"
                     /locus_tag="SDD03_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG4304973.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1886..3286
                     /locus_tag="SDD03_002562"
     CDS             1886..3286
                     /locus_tag="SDD03_002562"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG4304974.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3451..4891)
                     /locus_tag="SDD03_002563"
                     /pseudo
     CDS             complement(3451..4891)
                     /locus_tag="SDD03_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5284..5731
                     /locus_tag="SDD03_002564"
                     /pseudo
     CDS             5284..5731
                     /locus_tag="SDD03_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1836 a          919 c         1089 g         1887 t
ORIGIN      
        1 acaaaagtat cttcgattaa tacttgctca agttatgtgc agctacgaaa aagtaggtac
       61 cgaggtactt tctttttcgc agcttttttt ggacttatga gaagtacatt cgctggaaga
      121 atacgatata aaacgagctt tttcactaaa gatattaata gagtgaaaga aacaagcttt
      181 tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa aattctggaa gtggagaaga
      241 attttgcttg ctatactttg tcttgtagaa aagaggtgga caaaataatg caagctcaaa
      301 taagcgattt tccataacct tggtaggaat gacaaattaa ataggaaagg gaagtaataa
      361 tatgaataaa aaaaagttat ctctattcgg tttttttgcc ctgacagctt ccatggtttt
      421 gactgtttat gagtatccga cctttgcaac atcacaatta catttggttt tctttcttct
      481 tttgggaggt attttatggt ttctaccagt tgctttatgt gctgcagaaa tggcaacagt
      541 tgaaggatgg aaagatggtg ggattttcag ttgggttagt caaactttag gagaacgttt
      601 tggttttgca gctatttttt tccaatggtt tcaaattaca gtaggtttta ttacaatgat
      661 ctacttcata ctaggagcgc tgtcgtatgt aattaatttt ccagagctta atacagatcc
      721 atttatcaag tttattggtg tattagtcat tttctgggta ctgacttttt cacagttggg
      781 cgggaccaat cgtaccgcta aaattgctaa agcaggattt gtcattggaa ttgttattcc
      841 ttcgatcatt ctctttggtt tagcagcagc ctactttatc ggcggtaatc cgattcaaat
      901 ggctattaat gcgaaagctt ttattcctga cttttctcag ctttcaactt tagtagtatt
      961 tgtatctttt atattggctt atatgggcgt agaagcttca gcatcacata ttaatgagct
     1021 ggaaaatccc caaagaaact acccattggc aatgttttta ctagtgattt tggccatcgc
     1081 cttagatgca atcggcggtt tctcagtagc agcggttatt ccacaacatg acttgtctct
     1141 cagtgctggt gtaatccaaa catttgaaac gcttatttta cattttaaca gtcatttaca
     1201 ttggttggta aaagtgattg ctctcatgat tgcctgtggc gttatgggtg aagttagttc
     1261 ttgggtagtt ggtccatcac gagggatgtt tgcagctgct cagcgaggtc tgttaccagt
     1321 tttcttccgg aaaactaata aacaagacgt tccagttccg ttgattatga tacaaggaat
     1381 tatcgttact atttgggcag cagtcttaac ttttggtgga ggaggcaaca atttatcatt
     1441 cttagtagca atctccttaa ctgttgtcat ttatctcgta ggatatttac tatttttcct
     1501 aggttacttt gtattagttt ttaagaaaaa agacttaaaa cgaacttaca atgtaccagg
     1561 aaaaactgtt ggcaaaacga ttatcggtgg aattggtttt attttatcag tgttcgcatt
     1621 gcttatctct tttgtaccac cagcttcaat tgcagctagt gagcaccgaa cataccaaat
     1681 catactactt attagctttg tagtgactgt tcttttacct tttatcattt atgaagtaca
     1741 tgataaaagt gctcacacaa ctattgagga acctactcac gtgaaagctg aagacgttaa
     1801 tcccgctgtt tatccagctg ctagaggaga gcaccacgtt acaaaaaatg atgaacatat
     1861 tttaaaacat taggaggaat tcattatgtt atacggaaaa gataatcaag aagaaaaaaa
     1921 ctatttggaa ccaatttttg gctctgcaag tgaggatgtt gacttgccta aatataagtt
     1981 aaacaaagaa tccattgaac cacgaattgc ttatcaatta gtacaagacg agatgttgga
     2041 tgaaggaaat gcgcgattaa acttagctac tttttgtcaa acgtatatgg aacctgaagc
     2101 agtgaaattg atgacccaaa cgttagaaaa aaatgcaatt gataaatcag aatacccacg
     2161 aacaacggaa attgaaaacc gctgtgtaaa tatgattgct gatttatggc atgctccaaa
     2221 taatgaaaaa ttcatgggaa cttcaacgat cggctcttca gaagcctgca tgctgggtgg
     2281 tatggccatg aaatttgctt ggcgtaaacg tgctgaaaaa ttaggtcttg atattcaagc
     2341 aaaaaaacct aacctggtga tctcttctgg ttaccaagtt tgttgggaaa aattctgtgt
     2401 atattgggat gtggaactga gagaagtccc aatggatgaa aaacatatgt caattaatct
     2461 agatactgtc atggattatg tggatgagta cacaattggt attgtaggta ttatgggtat
     2521 tacttacact ggtcgttatg atgatatcaa gggtctgaat gatttagttg aagctcacaa
     2581 taaacaaact gactataaag tatacattca tgttgacgct gcatcgggtg gcttttatgc
     2641 accatttact gaacctgatc tagtttggga ttttcaattg aaaaatgtta tctcaattaa
     2701 ttcttcaggt cacaaatatg gtttggtata tccaggtgtg ggttgggtct tatggcgtga
     2761 ccaacaatac ttaccagaag aattagtatt taaagttagt tacttaggtg gagaaatgcc
     2821 aactatggct atcaacttct ctcatagtgc agcacaacta attgggcaat actacaactt
     2881 tgtacgctat ggctttgatg gttatcgtga tattcaccaa agaactcatg atgttgctgt
     2941 ttatttagcc aaagagatcg aaaaaactgg tatttttgaa atcattaatg atggatcaga
     3001 attgccagtt gtgtgctata agctgaaaga agatcccaat cgcgaatgga cactatatga
     3061 tttatctgat cgtctgttaa tgaagggatg gcaagtccca gcctacccat tacctaaaga
     3121 cttggatcaa ttaattattc aacgcttagt tgttcgagca gactttggaa tgaacatggc
     3181 tggtgattat gtacaagata tgaaccaagc aattgaagag ttgaataaag ctcatattgt
     3241 ttatcataaa aaacaggatg tgaaaacgta cggctttact cactaaaatt cttcagagaa
     3301 atataaaaga agttaattaa ctaataaaag gagttgtgat taccacacag ctccttaaat
     3361 ctatttgaat aaatatatga tattcatttg ttacactctc aatcttagct gttgttctat
     3421 tcatcattac tcttattgta agtagttcag tcaaatttgc ttgttcaatt gatcgataac
     3481 agtatgaatt gtttgaaaag taatcggata tataattgtt ttctcactac ctaagccttc
     3541 ttggtataaa gaactatcgc aaagtataat ccgctgagta caaaagaaag tgtcgtctac
     3601 tatttctcta agttgtttac aaatgatatt actaatagaa aagtgttctc ccaaaatgtc
     3661 ttctaactgt tgtcgatagt ataaatacct aaagtctgac ggagctacta tgaacacttc
     3721 aattggagat ttccgtttat gctcatttaa aagacttaaa ctaatggtta gtttacgaat
     3781 agaattctca ttccaacgca gaaataaatt gttttgtttt cgccattgtt caagaatttc
     3841 atgaacttct aagtacaaag cgcgttgctc ttcatttaac aagtaagtct tatcaggttg
     3901 aaatatctga atatcagccc aaagtgagcg gataaacggg agaagtgctt tatcaaaaag
     3961 tatctgattt tcttcagata tattcagaga taagataagt ttttgactga gctcaatcac
     4021 caaagggtta tctgctacat ggttttgata aacaacgttc aagtcttttt gtaacaattc
     4081 aaagttatcg catagataat ttctagagtt gaatagaaag tagatataaa ggcattcatc
     4141 ttcgtcatga cataaagaga aagaaagttg ctctaatcct ttctgtatta gctgaaacaa
     4201 gggagtatgt cgggcaactc ttttttcttc ctgactaaaa tatattgggt tagtcttaga
     4261 tctctgcaaa ccgatagcga tacctaataa aataaattgt ttttcaactg tagggtaact
     4321 tcttgaaaaa aaatgttttt ctacataatc aatcaattgt tgaccgcttt gaataattga
     4381 tttatttagc tggatcaact ctgtccgatt tgtctcatag attatcgtta gtaacaaact
     4441 tcgataatcc ttttcaggaa tttcaaaatg gccatcagca tttaagtact gcatttgttt
     4501 aaaaaaatta atcaactcat ttcgtaagtt gtaaactttg cttaacgaaa tgaaggtagt
     4561 atcagctata ttactccaat tgtgttcccc tgataaataa tgtcctagta cattgaaaaa
     4621 gtcagagcgc tgatagatga gttgagtgag tgccaacctc gaaaacgaag agtcgaatgc
     4681 gactgtaaag ctacttctag atttctccac tgaaataatt tgctgatgga gtatgtcggt
     4741 aatgcattcc aagtcgttag tgacagacag tgagtgggca aacgttcaat tgatctgcta
     4801 tttcgcgaat ctgaatggtc cctttttcgt gtaattgctc acaaatgtaa acttgacgaa
     4861 aaatactttt ctccagatag gtttccaaca tagtactcct cctagtcatt ttatacataa
     4921 ctattataac tctaccaatc ggaatagtcc tgtaatatat tgaatcaaaa atataaatta
     4981 taaaataatt agatggggta tttggtttaa gtatccctta tatagtaata aatttatagg
     5041 tgtgattctc gaattaatac ccgaaaatgt gagagaattt ttatggattt tttaggatac
     5101 aaattaatta tttttgcttt tatcctgatt accagtccag tactgtcggt atcccatttt
     5161 gcagcaaaat catgttgata aagtgacaag aagactgatg agatatatta tccaaattga
     5221 gaagataatt aatcggattc ttgagtatac cctacttttt aagaaagtag ggtatacaag
     5281 cgtatgaata aagacataac tttccctgtg atggacgttc ggcttgttcc aattaccaaa
     5341 atacaggcga acgattataa tcccaatcga gtagcgtctc cggaaatgaa cttattggag
     5401 ctttctatca ttgaagatgg ctttactcaa ccattagttt gttattacaa ggaaaaagaa
     5461 gagaagtata tcttggttga tgtgcttcat aggtatcgtg ttgctaagga aagactcaac
     5521 ttaaaagttg taccggtaac agttattgat aaacccatat aagaacgtat ggcttcaaca
     5581 attcgacaca atcgtgcaag agggacccat gaaataagag gaatggaaat tgtcatccaa
     5641 agattagtaa aaaatggatg gtcagacaag agaatatcca aagaattagg catggacatt
     5701 gaagaaattt ttcgctttaa gcaagtgtct g
//
LOCUS       DARDHU010000128         5559 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000128 DARDHU010000000
VERSION     DARDHU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.130"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..2009)
                     /locus_tag="SDD03_002565"
     CDS             complement(60..2009)
                     /locus_tag="SDD03_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG4304975.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="SDD03_002566"
     CDS             complement(2051..3109)
                     /locus_tag="SDD03_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG4304976.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="SDD03_002567"
     CDS             complement(3228..3683)
                     /locus_tag="SDD03_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304977.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="SDD03_002568"
     CDS             complement(4267..5070)
                     /locus_tag="SDD03_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4304978.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1631 a         1101 c          768 g         2059 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttg
//
LOCUS       DARDHU010000129         5531 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000129 DARDHU010000000
VERSION     DARDHU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.131"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..985)
                     /locus_tag="SDD03_002569"
     CDS             complement(<1..985)
                     /locus_tag="SDD03_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG4304979.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="SDD03_002570"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="SDD03_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG4304980.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="SDD03_002571"
     CDS             1810..2964
                     /locus_tag="SDD03_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG4304981.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="SDD03_002572"
     CDS             3179..4147
                     /locus_tag="SDD03_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG4304982.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="SDD03_002573"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="SDD03_002573"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG4304983.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5531
                     /locus_tag="SDD03_002574"
     CDS             5177..>5531
                     /locus_tag="SDD03_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG4304984.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1669 a         1078 c         1251 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc g
//
LOCUS       DARDHU010000130         5365 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000130 DARDHU010000000
VERSION     DARDHU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.132"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..324)
                     /locus_tag="SDD03_002575"
     CDS             complement(<1..324)
                     /locus_tag="SDD03_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304985.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQ"
     gene            complement(622..1596)
                     /gene="sgrA"
                     /locus_tag="SDD03_002576"
     CDS             complement(622..1596)
                     /gene="sgrA"
                     /locus_tag="SDD03_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG4304986.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1792..2631)
                     /locus_tag="SDD03_002577"
     CDS             complement(1792..2631)
                     /locus_tag="SDD03_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4304987.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2816..3703
                     /locus_tag="SDD03_002578"
     CDS             2816..3703
                     /locus_tag="SDD03_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG4304988.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4287..4982)
                     /locus_tag="SDD03_002579"
     CDS             complement(4287..4982)
                     /locus_tag="SDD03_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG4304989.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4966..5364)
                     /locus_tag="SDD03_002580"
     CDS             complement(4966..5364)
                     /locus_tag="SDD03_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG4304990.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1713 a         1083 c          787 g         1782 t
ORIGIN      
        1 ttgataagta cctttgtaaa gtgaatccag taaaatataa ccaatcgttc cctctaaaag
       61 tatagaataa actttactca gtaaaaactg agaagaaaac tcgactttta tcatcttgtt
      121 ttctgaaaga aaagataatt tatattctgg atacctttcg caaaacgatt ctgcttgttg
      181 aatcaccgaa ttcatcttag ccagggtgta gtttagatct aaagcgatat ccttcaccac
      241 atataatttc tcgtctcttt cataacaata gttcaaaaat tgatatactt gcctttcatt
      301 gatatcaata aattcttcaa acaaagtaca tactcctttt tatctattat ttagacttat
      361 tccgacagct acatgaataa cgtacaaata gattcgttac tgatttttca gagatactac
      421 catgcgcagt gtgcattgtc aattattctt gctcttattt tccatttgtg ttcctgttat
      481 ttcctgtttc tcaaatatgt tagtaaacaa aaatagatct ggcaaagcta caacttcgcc
      541 aggcaataca gcactctcga atgaaatatt cactcaaaaa tctatcgttt tattcaattt
      601 tagatctgtt gcttttattc tttatttcac tcgatggaag agagaacgaa aatagcttgc
      661 tcctacaatc aaaatgagta acacacccat ccatgtaatt agcttgttca aggttctact
      721 accagtttct ggtaaatcgc tttttctatt atctgtttct ttcgcttttt cttgatgatc
      781 aattccacga gaaacaaaat caggctgttt tagcacactt tttaggttgc tccctgcatt
      841 tctcttttgt tccttagttg gtatgatagg taccaaatca agttcattat ttacttttcc
      901 tgtgctgctt tcgctgctcg tgcttcctgt gctactctca ctactcatgc ttcctgtgct
      961 actttcacta ctcgtactct ccgtactgct ttcgctgctc gtgcttcctg tgctactttc
     1021 actactcgta ctctccgtac tgctttcgct gctcgtgttt tctgtgctac tttcactgct
     1081 cgtactctct gtactgcttt caccgctcgt gttttctgta ctgctttcac tacttgtgct
     1141 atccgtactg cttggcggtc caacaactgt ccaactgctt ggtttcaaat tctccgtgat
     1201 cgtcatctgt ccatcataaa aaacaatcaa atcaacttta tctttactct tgatctcatc
     1261 aatcgatagt tctgcagaaa tcattattgt atactcaata ccagcggtcc attgtacgtc
     1321 ttttaaataa acttccccaa taactttttc tgaacttcca ttaccatccc aaccatatgt
     1381 tagaacagca tgtacatcac tagcattcac gcttttgttt ggtttgactg gaatagtaat
     1441 gacttgttgt gtcatgtcat aaacaatccg atcagttttc acagtaaatg aaagattctc
     1501 atttgcccgt tcatttgcag aagtactgat tccaatcatt gctgcaatta tactcagccc
     1561 aagaataaca aataatacgg tcgctgtttt cttcatttct atcactctcc atataattac
     1621 cttctatttg ataaaggaat tatataaaat gaaaatgatg agaacacgac agtcagttgt
     1681 gaaaatctaa caaaagatgt cgtgtattcg cttgaatttg tttacttttt cgtttaatca
     1741 atgacagact taaaaaagca agaccgatat cagccttgct ttcttcatct tttattgata
     1801 aacagcagtg tatttcttca tccgctttgt tttatatgca tagagtaaca aaccaacaat
     1861 caagactaac aagaataatg tagttgatat agatgcttca gccccaaagt ttcccccagt
     1921 aattattgta ttttgcgatt ggatcagagt ggagaaaaaa ccttgttctt gcgttccgct
     1981 gactggtata cccaaaatag ctccttgtgt ccaattccaa gtaatatgag caccaatagc
     2041 cgtaaataaa cttcctgtac tgtagataag tgcgctgaaa aagcaacctg ctaaaaagat
     2101 attgaaaact gccagaaatg tcatgttagg attcaatccg tgcaaaagag caaataatgc
     2161 agcagatcca atcattgcta ttaacggacc aaatctaagc atctgatgct gaaaaaagcc
     2221 acggaataaa agctcttctg atgcccccac tgcaataaat aaaataaaac tcgttatcag
     2281 cggcacaatg tttgcgtttc ctgaatctaa ccgatacgcc cccgtcaaat ataatggact
     2341 agtaataagc agaaacactc caattccaac aacgagacct cccgctaaat acccccagtc
     2401 ttttcctcga acagaaacac cataatcttc tattcttttt ccttcgacct tactagcaag
     2461 aaataaagct agtaaaaaaa cagctaaatg tccgaccgta ccggcaattg ctccaatcca
     2521 ttcactattt ccaataaaca tatccgcaag tgcattattg acaagaattc ctaaaacaaa
     2581 accaactaag actattaaaa cagatccaat caaccgaata atcctgatca tctttcatcc
     2641 ctcctttata ttaagtttaa caaccctttt ctttcttgtc tcgtgaaaac tatgtaaatg
     2701 ttctccatca tagagagaat agatttttac gaactaagaa tatctacagc aaccttacga
     2761 aaatgaaaga ctgtctccct aattcttctg ttagactgtt tttaataagg aaaggatggg
     2821 aaattttttg aaaaaacaac tactaattat gtgcctttca gcatgtgcaa ttttactatt
     2881 ctcaggttgt agcaagtcca atgaaacgat tgcaacgttt aaaggtggaa cacttacagt
     2941 tcaggatttt tatgataacc caaaagttaa atcaaataat caaacactat taaaaaaaat
     3001 gattgtcttc aaagcatttg atgatgtata cggaaaagag atttcttcaa aggaagtaac
     3061 aaaagaatat aatgaacaaa tcaaaaaact cggtccaaac tacaaggaac aattaaaagc
     3121 agttggccaa acagaagaga catataagaa actctttaaa cagatgctgg ctttccaata
     3181 cggtttgaaa gccaatgtaa aattaacaga taaagatcta gacactgctt ggaaagaatt
     3241 ctatccagaa gttagtacac agatcattct attttcgact aaagaagaag cagataaagc
     3301 aaaaaaagaa gcaaatgaag gggaaaattt ctccaagctt gttcaagctt atggaaaaaa
     3361 taaaacatta aaggaaactg atggaaaaat gaattttgat tcaaccaatc cagagattcc
     3421 tactgaagta aaaaaagcag catttaaact aaaaaacggg gaagtttctg atatcattcc
     3481 tgtaacagat ccaacgactt atcagcaaag ctattatcta gtaaaaatgg taaaaaaaca
     3541 ggataaaggt tctaataaag acaaatacaa atctgagcta gaaaaaattg ctaccgaagc
     3601 aaaaactctt gatacggaat tcatggataa aacaatccgt aaagtgatga aaaaagacaa
     3661 tgtaactatt aaagatcctt atgtaaaaaa tatattcaaa taaacaaatt tgacccaatt
     3721 ggggatgtgg caaaatttga gaagtgattt ttggaaattc cttcttattt ttgaggcaaa
     3781 ccacttttgc cacaacttca tcggggcctt tttttatttg taccaaaaca aatcactgga
     3841 tttgttacaa tcatgaggat ataatttaaa aaagtttttt atttctatca catgaatcaa
     3901 tgcgccatta agtagattct ttcatgcttc cctatttttt cacttgccgt atcgcttcaa
     3961 aaacaactat cataaactca aactcgactg aaaagttatt ttaaaaaaga ctaaaaatat
     4021 cccttctgga cttgttcctc ttattctgtt atactagaga aacacgaaag atatttttat
     4081 gcgacacttt tcatttgttt ttatgaaatt tattcaaaac tggcgtaact tcagagttac
     4141 aaggatgtat tagaggttag ggaatacatc gtttaaatga gaaataagta atagtcaccc
     4201 aaactttaaa ttaatgaata ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct
     4261 atcgtttatt ttatggagaa attgattcag aagtaatcag cgaccttttt tagttcacca
     4321 atcgtttctg cttggaaagt ttgcacccaa tcttcttgga agcccctcac tgctttatca
     4381 atcgagggca tctctaatac ttgatctagt agctctttac ctaacgttat cgcatgagct
     4441 ccagatcgaa tggctgtagt gacttgttca caatttttga aactagctgc taatattttt
     4501 gttggtcttt gcgtacgttc gatctcagtc cgcaacgaat caatcacttt catagcatca
     4561 atgccttgat tttgcatcct gttataataa ggtgcaatat aatctgctcc attattaata
     4621 gctaaatacc cttgtaattc tgtgtaaata gctgtaccgg taatcaaata accttctttt
     4681 ttcaactttt tgatcgcttg aaggcctgct tgattcaccg gcaccttgat aaacgtatct
     4741 tttcccagtt cttctatgat aactcgagca tcctctatca tttcttcttc cgttcttcct
     4801 attgtctgaa tatggatagg aatctgtccg attatttttt tgatttcttt catatgcgag
     4861 aagaagtttg acgctccttc ttttttcaga attgtaggat ttgttgttac gcctgttaaa
     4921 ttcaaaaact gttgacaaga ataaatactt tttgtatttg ctgtatcaag catgatttcc
     4981 atatagcttt cccccattct gatcctcttg atcgatttta atgattgtcc cctttcttaa
     5041 atgcggcatt tccttatctt ctacatacat tgttcctgga agttccggct catgagatcc
     5101 atcaaatttt atcgtgatat gtcctaattc attcaaatta gtttggacaa catctcctac
     5161 tgctgtgatt acatacattt gtttatcaaa acagagcttc atatcacaag ttatttcttt
     5221 tcttacctga ttcaattcaa tcttataaca ataatctgtt aatgtttccg gtgcttcatc
     5281 tccaaataaa atcattattt tttctttcct gaatacttct gcttctatac caacatctgt
     5341 tacaaccgtt tcaaagatcg tcatc
//
LOCUS       DARDHU010000131         5341 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000131 DARDHU010000000
VERSION     DARDHU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.133"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..280)
                     /locus_tag="SDD03_002581"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="SDD03_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="SDD03_002582"
     CDS             complement(546..740)
                     /locus_tag="SDD03_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4304991.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="SDD03_002583"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="SDD03_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="SDD03_002584"
     CDS             2092..3132
                     /locus_tag="SDD03_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG4304992.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="SDD03_002585"
     CDS             3956..4288
                     /locus_tag="SDD03_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG4304993.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="SDD03_002586"
     CDS             4907..5248
                     /locus_tag="SDD03_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG4304994.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1771 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DARDHU010000132         5228 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000132 DARDHU010000000
VERSION     DARDHU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.134"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..794
                     /locus_tag="SDD03_002587"
     CDS             <1..794
                     /locus_tag="SDD03_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG4304995.1"
                     /translation="LKKLELSQSDFIRIKDYCIQNKIDFLSTPFDEESADFLKQIGVQ
                     GFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNY
                     PASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPG
                     PDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGD
                     ILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE"
     gene            791..1939
                     /gene="neuC"
                     /locus_tag="SDD03_002588"
     CDS             791..1939
                     /gene="neuC"
                     /locus_tag="SDD03_002588"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG4304996.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1912..2589
                     /locus_tag="SDD03_002589"
     CDS             1912..2589
                     /locus_tag="SDD03_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG4304997.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2579..3721
                     /locus_tag="SDD03_002590"
     CDS             2579..3721
                     /locus_tag="SDD03_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG4304998.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            3734..4711
                     /locus_tag="SDD03_002591"
     CDS             3734..4711
                     /locus_tag="SDD03_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4304999.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            4711..>5228
                     /locus_tag="SDD03_002592"
     CDS             4711..>5228
                     /locus_tag="SDD03_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG4305000.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCI"
BASE COUNT         1919 a          653 c         1004 g         1652 t
ORIGIN      
        1 tgctaaaaaa attagaatta tctcaatctg attttattag aataaaagat tattgtatac
       61 aaaataaaat tgattttttg tctactcctt ttgatgaaga aagtgcagac tttctaaaac
      121 aaattggtgt acaaggattt aaaattggct caggagattt aacaaattta ccttttctta
      181 gaaaaataga tatgtatggg ttgccagttt tattatctac aggtatggct gatattcatg
      241 aggtcagtga ggctgtagat tgtttcatag atagtccagt tactatttta cattgtactt
      301 caaattatcc ggctagttct gaagatatta atttattggc gattaacaca ttaaaagaaa
      361 tgttcgggat acccgttggg tactccgatc attcattggg atatgatgtt ggtatttgtg
      421 ctgttgcgat gggtgctaag gtaattgaga aacattttac cttggataag gaactacctg
      481 gtccagatca taaagcttct ttagatcctc aggaatttgc agattttgta aagcatatta
      541 gaaacactga aatattttta ggtgatggtg ttaagcgagc aatgccttct gaacttagta
      601 caaaagaagt atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg ggagatattc
      661 ttacagagaa atctcttaca ataaaacgtc ctggaaatgg aatagctcct aagtttttat
      721 atcaatatat tggcaaaaaa gttaaaagac caataagtgc agatagtgtt ctgcttgagg
      781 aagatgttga atgaaaaaaa tacttatagt aagtggaaca cgagctgatt atggaatata
      841 taaacctgtt gctgagaatt tactacaaga taattgggat gtatcatttg ttgtttctgg
      901 aatgcattta tcccataaat atggatttac taaagaattg atcttagctg acggatttag
      961 aattagtgga gaagttcaaa gtttgttttt agagaacaat aagggaaaca tggctcggtc
     1021 tgtggcatta gagataaatg gtttcacaaa tatatttgaa caaactagcc ctgattttgt
     1081 attattacta ggggatagag gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg
     1141 aatcaaaaca gcacatattc acggaggcga agtgtctggt actgtagatg aatcaataag
     1201 acatgccata acgaaaatag catctgttca ttttgctgct acggaaaaaa gcaaagagcg
     1261 tttaataaaa atgggggaag attcttggag agtttttccc gtcggagctc ctagaataga
     1321 tactatttta aataatagat tacctagttt tagagatata aaagaaaaat atcatctaaa
     1381 atttgaaaag aaaaaatacg ttttgcaagt atttcatcca gttgtaaccg aactagaaaa
     1441 tatagaaaaa caaattttag ctttgatcaa ttcaatggaa caacaatctc aaccaattat
     1501 ttgcatttta cctaatagtg atgctggtag cgaaattatc agaaaaatgt accagaaata
     1561 tttaaaaacg gatattattt atattacgaa tttagagccg gaggaatacc tgactatatt
     1621 aaaagagtgc aaatttatga tcggaaattc ttcttctggg ataattgaag ctgcatcatt
     1681 caaaatacca gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat
     1741 tataaatgca aatctaaatg aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc
     1801 tcagtttata aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag
     1861 aagaataaca aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca
     1921 aaaacaaata gcttattaaa atatactagt gatccataca ttattattgg ggctggcgga
     1981 cacgcaaaag tcattattga aaccttaaga ttagaaaata agttaatata tggatttttt
     2041 gatgataagc aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat
     2101 agctgggcgg atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta
     2161 gtaagaaaaa agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt
     2221 catccaagtg ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg
     2281 aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc
     2341 attgttgagc atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta
     2401 gcgggtggag tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag
     2461 atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt
     2521 ccctcacatt ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt
     2581 gaattctaga attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc
     2641 taaggctttt aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga
     2701 aagtattaca aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc
     2761 tgccatacat cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc
     2821 ttcatttact tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt
     2881 cattgactca gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga
     2941 agaagctgaa agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca
     3001 aatggctgat tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga
     3061 agatgcagcg gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac
     3121 aattgggatc tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct
     3181 agtgtctagt gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga
     3241 tcaagcaccg tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc
     3301 tgccggtatc ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga
     3361 aattttcaat cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga
     3421 agtggatggc atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc
     3481 tattgatcaa gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc
     3541 tttgtggaaa ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga
     3601 taataatctt agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag
     3661 taatatgaca gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta
     3721 ggtgtgatat tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact
     3781 tagactcatt caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc
     3841 gagacgaaag gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc
     3901 aaaaaatttg tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt
     3961 tattataaaa gaggctttaa atttagattg taatatcttt acggaaatca atttattaaa
     4021 tgaatattat gatgaaataa tcgaagtagc taaaaagaaa gatagattac tttatctatc
     4081 ttctacattt ttgaagagaa aagaaattca atatattaag tctaaagtga cgcataataa
     4141 aaaaatttct tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga
     4201 ttatcgcaat ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat
     4261 tgaattacct tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca
     4321 gaaattatcc aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga
     4381 aactggtata gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt
     4441 aactataatt ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc
     4501 atggaatgaa gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga
     4561 taataattat tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt
     4621 cacgcttttg gaaaagaaaa tggaacccca atatactttt gaaaaagata agtatattat
     4681 taacttaatt aacgacattg aggtaaatta atgatgaaag taattttttt tgggttaggg
     4741 tcaattggaa agagacatct aaaaaatcta ataagctatg gaaaaaagaa ttctattaat
     4801 tttgatataa cagcttatag tagcagtgga aaaaaagagt ttgaagaaat aacgtatatt
     4861 caaaatagag acgatttgga tacacattat gatatagctt ttattacaaa tccgactttt
     4921 aagcatctgg aaacactaaa tgtaattgtt gaaaaagttg attacatatt tttagaaaaa
     4981 cctgcttttt ctgagagtat tgatcttact caatataatt atgattacaa aaatatttat
     5041 gttgctgctc cactgaggta taaaaatatc atggaagttg ttcgtaatag tattcaaagt
     5101 aaaaaagtat attctgtaag agctatttgt tctacttatc ttcctgattg gagaaaaggt
     5161 actgattata gaaataatta ttctgccttt aaatctatgg ggggaggagt agaattagac
     5221 tgtataca
//
LOCUS       DARDHU010000133         5154 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000133 DARDHU010000000
VERSION     DARDHU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.135"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SDD03_002593"
     CDS             8..649
                     /locus_tag="SDD03_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305001.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SDD03_002594"
     CDS             708..1148
                     /locus_tag="SDD03_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305002.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SDD03_002595"
     CDS             1160..1828
                     /locus_tag="SDD03_002595"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG4305003.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SDD03_002596"
     CDS             1892..2515
                     /locus_tag="SDD03_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG4305004.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SDD03_002597"
     CDS             2571..2957
                     /locus_tag="SDD03_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG4305005.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SDD03_002598"
     CDS             3170..4561
                     /locus_tag="SDD03_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG4305006.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1708 a          861 c         1084 g         1501 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agat
//
LOCUS       DARDHU010000134         5061 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000134 DARDHU010000000
VERSION     DARDHU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.137"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..660)
                     /locus_tag="SDD03_002599"
     CDS             complement(205..660)
                     /locus_tag="SDD03_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305007.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="SDD03_002600"
     CDS             complement(1063..1236)
                     /locus_tag="SDD03_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305008.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="SDD03_002601"
     CDS             complement(1917..2456)
                     /locus_tag="SDD03_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4305009.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="SDD03_002602"
     CDS             complement(2472..3395)
                     /locus_tag="SDD03_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4305010.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="SDD03_002603"
     CDS             complement(4523..4678)
                     /locus_tag="SDD03_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305011.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="SDD03_002604"
     CDS             complement(4697..>5061)
                     /locus_tag="SDD03_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4305012.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DARDHU010000135         4859 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000135 DARDHU010000000
VERSION     DARDHU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.138"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="SDD03_002605"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="SDD03_002605"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG4305013.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="SDD03_002606"
     CDS             1085..2005
                     /locus_tag="SDD03_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4305014.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="SDD03_002607"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="SDD03_002607"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG4305015.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="SDD03_002608"
     CDS             3311..4789
                     /locus_tag="SDD03_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4305016.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1706 a          780 c          938 g         1435 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaac
//
LOCUS       DARDHU010000136         4842 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000136 DARDHU010000000
VERSION     DARDHU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.139"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(182..1144)
                     /locus_tag="SDD03_002609"
     CDS             complement(182..1144)
                     /locus_tag="SDD03_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4305017.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="SDD03_002610"
     CDS             complement(1146..2585)
                     /locus_tag="SDD03_002610"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG4305018.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="SDD03_002611"
     CDS             2770..4716
                     /locus_tag="SDD03_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG4305019.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1551 a          819 c          791 g         1681 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga at
//
LOCUS       DARDHU010000137         4785 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000137 DARDHU010000000
VERSION     DARDHU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.140"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..522
                     /locus_tag="SDD03_002612"
     CDS             <1..522
                     /locus_tag="SDD03_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4305020.1"
                     /translation="QYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTA
                     IFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIY
                     SEKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPELK
                     KGLLDNLQNLFKE"
     gene            532..1029
                     /locus_tag="SDD03_002613"
     CDS             532..1029
                     /locus_tag="SDD03_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4305021.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            1405..1686
                     /locus_tag="SDD03_002614"
     CDS             1405..1686
                     /locus_tag="SDD03_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305022.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            1688..2104
                     /locus_tag="SDD03_002615"
     CDS             1688..2104
                     /locus_tag="SDD03_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305023.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            2756..3181
                     /locus_tag="SDD03_002616"
     CDS             2756..3181
                     /locus_tag="SDD03_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305024.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            3211..3375
                     /locus_tag="SDD03_002617"
     CDS             3211..3375
                     /locus_tag="SDD03_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305025.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            4333..>4785
                     /locus_tag="SDD03_002618"
     CDS             4333..>4785
                     /locus_tag="SDD03_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4305026.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLL"
BASE COUNT         1755 a          817 c          785 g         1428 t
ORIGIN      
        1 caatacagaa ccaaaggaat tgcctatttt tcaaaagaag actttcatga attacttgat
       61 atccctaaaa gttatagaga aagtgatata aataaaaaaa ttataaagcc tattcgccaa
      121 gaactaaccg caatcttcaa aggtctgaca atcaaaaaga aatacggaaa aggaagagga
      181 aaacctgtca ttggctatca attcacgttt aaacctgaaa taaaaaaagc agacgacttt
      241 aataaacacg caccaacaaa agaagaacaa aataaagctt tagaactagt taatcaacaa
      301 atttactctg aaaaagaccc cacttcatta caagaaaaaa tcaaagaaca gaaacgaaaa
      361 ttagaacttt tacaacaatt agaagagcta gaaagaaaac aaaataatct gaataaagaa
      421 aataaggaga ttaacgaaca aataccagat aacaattccg atattccccc agaattaaaa
      481 aaaggcttac tagataatct tcaaaactta tttaaagaat aaggggtttt tatgacagat
      541 aaaactatca aagaattagc agatattcta ggtgtttcaa aagataaagt gaagtatcaa
      601 ctgaggaaat tacctagtaa ttttacctac aaaaaaggaa atattatcta cctgaaaaaa
      661 gaagcagtaa caacaattac aaaggtttta tcaggtaatg agccgggtaa cttacctagt
      721 gaattacctg attcattacc taccgattac ctactcaagg agattgaaac taagaataat
      781 catattgaag aaaaagatag gcaaattgaa aaacttcaac tagctttaga aacacaacaa
      841 aaattactcg accagcaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
      901 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
      961 tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg agaaaaaatg gtggcaattc
     1021 tggcaataaa accagcaatt taaattaata aaaattgctg gttttattct tctttttgct
     1081 tttgcgagca gagcaacatg ggcccatgtt gcgatatttt ggtggcgccc caatccccgt
     1141 tttttcgttg aatcttactc ggactgcact cgttttacag ctgatttttc tgaattttga
     1201 aagagcataa ggaaacgtgg tatcactttc ttacgaaagg tgctagataa gctgtgccta
     1261 tcacgtttct cagaaaaaaa tttgctatca cttttttgcc attgtggcta ggctatgtat
     1321 agtgttttgg cgaaaaatag ctatacaaat actatatttg tatgatatac ataaattata
     1381 tactgatacg aaaggaaaat ttccatggac attttagttc gaaatatcaa tcccaaaatt
     1441 attacaagaa tagataaacg ttgtcaggaa ctaacaacca aaacgggcaa aaaatggagt
     1501 cgaaatcaat acctaaaaat tttaatcgaa aacgattttg accacgaact tatgaattac
     1561 aaaaaagatc aattcgattt attattcgaa aaatttattg ccattcaagc agaaaatacg
     1621 aacatactaa atgactatgt tcacacaaac aaccaactca ttgaaacact tatcggagga
     1681 atataacatg agaaaaactt ctgttcaaaa acacgtaacc ctttatccca gtcacgttga
     1741 cgttttagat tccatcatcg aaaaaaatcc agacgtcaac aattattcag aagccattcg
     1801 ctttttatgt atgagttatg aacctgctac caatacaacc acaaccagta accaagcaaa
     1861 actcaatgcc atgggaaaag aaatttctat catgacagaa atcatggcag aatttgctga
     1921 tttatatcta aaagatactg gtgttcttat cggtacaaat tctaaagtat atcaagaagt
     1981 gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga aatcaaccca aaaatcgtca
     2041 aaaagaaacc aatcaaacct ttacttctgg aaattttgac ccaacaaact ttctaaaaag
     2101 ataaaaaaag aaaccgaaaa ataaaaaaat tcctcgattt ctttttaggg atcggtcttc
     2161 cagcaagatt tgcaagttgg taccaacttg cgaaaaaaaa aagaaaaatc cgcttctgcg
     2221 aattcttctt ttttcatttt ggggcgctgc cctaatccct aagtcccggt tattcaatag
     2281 catggaaact caaagaataa gacgttccct ggaataaaaa aaaggctgct cgttcgagca
     2341 gtccatacat aggaaaatga aaaaacgacg tcccttactg agaaacgcta ttaaactttt
     2401 aacaaatcct ctaaaaaata acaagtcttt aaaaagtgac cggtaaaaat gcacaaagaa
     2461 ctaaaaaaga gaaattgcat caaatcaagc attagaagct agtaactagc tagaccaatt
     2521 gaaaatcaca aagtgagcgc ctgtatctat tgctacaggc gctacttggc atagtacaca
     2581 ttatgtcaac aagaatttaa tataacatat ttttctagtt tgttataaca atgccactct
     2641 aacaaaaatg taagcattat ccttattaaa agctccttga caaggactat accttttatt
     2701 ttatgatttt tacatagaaa acgttttctc tattttttgt ttagaggtga tttttatgat
     2761 ttgcaagaac aagaaaaaaa ttattgctat tattgctagt ttctctttga tttttagtac
     2821 tgcatgtact gtcttattaa caaataatgc taatgttgaa gctgcaagct cttcttacaa
     2881 acataatcat gctggttata gatgttacaa aatgagttat tatgtaagtc cttctaaatg
     2941 caaacaattg gctcgccaaa ctaataaact taataccgtc tcagctattg caaactttat
     3001 tggactatct ggattaacac cggggattat atcattagtt ttcggatcag cagttagtgc
     3061 taataatgta tttgttacag ccgctagaag aggaaaaggc gttcaattaa attatattgc
     3121 ccattttagt aataccacta cctataatta tagttctaac caaaattatg ttataaaata
     3181 attaaaactt taaactagga ggaatgcttt atgaaattaa caaaaattgc tgtaatttta
     3241 gctgttattg ctggcattat tttatatttt gtattgcctg ctaatcaatt cttcctttac
     3301 tttggaatac ctgcgatttt aatagtaaat ggcgtaagaa tctttgaaca acgaaaaaaa
     3361 tgtagctcta cctaatcgtt acaaaacaac aatttcacct tcggtgaaat aagcgaaact
     3421 ttacaaaaag tggctagcaa atgctagcct tttttgtatt gtagagcgtg aattttgagg
     3481 gggattcaag ggggagcttg cgcccctttg acaagcggtt atttgcacca aatacctccg
     3541 tatttggcta gcaatccttc taaaaaatac acctatcgat cagagtcaaa acaaatcaat
     3601 tttcgagtga gcgaatccga atttgcaaag ctgaagcttt gcaaatgagt gtgccggctt
     3661 ccgtaaaaat acatatggct ataatttgcg ttttaaggcg ttttaaacgt tctggtatat
     3721 aattatacca aaacaacttt aaaacgcctt agatctaatt ttagaggtct tatagaacat
     3781 tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttgagaac aagataaggg
     3841 ttttatacta agactgtctt aaaacggctt aaacaaacgc tcagaacttt tcaaaggagt
     3901 tctttatgaa ctgtttttaa gcaaaggagc ctttaggcga ctgcgcagta taatgtgaga
     3961 gcgtagcgat cacacatttt tagtgaccgt agggagcgag tttcccagtt tagaaaaatt
     4021 cgcgaacttg tttgaggaaa aaagcaggag ttttttaggc gggaaatttt gcctcctctt
     4081 ttataatctc tttttaaacc tctttttaaa cctctttacc gtcggctgta tgcttactct
     4141 cccaaggtgt gcagaatctt ttgggggtct ttttgtctgt ttttaggggt ctttttgtct
     4201 gtttttaggg gtctttttgt ctgtttttgg gggtcttttt gtctgttttt aggggtcttt
     4261 aattaggggc ttgaccccta ttagaaagct ctttataatt ttgttaagta gttaatcttg
     4321 aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa tacgattcct
     4381 ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctatagtatc tagaatgcgt
     4441 gacgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt aagcaattat
     4501 aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga caaattaatg
     4561 gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt tgttttattt
     4621 aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca agtccatgag
     4681 aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct ccaacaattc
     4741 aatgaattag aaagcagtta ttccaaaacc atgtttagat tatta
//
LOCUS       DARDHU010000138         4775 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000138 DARDHU010000000
VERSION     DARDHU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.141"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="SDD03_002619"
     CDS             complement(<1..1128)
                     /locus_tag="SDD03_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG4305027.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="SDD03_002620"
     CDS             complement(1164..1874)
                     /locus_tag="SDD03_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4305028.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="SDD03_002621"
     CDS             complement(1942..2757)
                     /locus_tag="SDD03_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG4305029.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="SDD03_002622"
     CDS             complement(2768..3736)
                     /locus_tag="SDD03_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4305030.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>4775
                     /locus_tag="SDD03_002623"
     CDS             4499..>4775
                     /locus_tag="SDD03_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG4305031.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT         1473 a          983 c          726 g         1593 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagt
//
LOCUS       DARDHU010000139         4754 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000139 DARDHU010000000
VERSION     DARDHU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.142"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1131)
                     /locus_tag="SDD03_002624"
     CDS             complement(<1..1131)
                     /locus_tag="SDD03_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4305032.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDD"
     gene            complement(1261..2637)
                     /locus_tag="SDD03_002625"
     CDS             complement(1261..2637)
                     /locus_tag="SDD03_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4305033.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2637..3293)
                     /locus_tag="SDD03_002626"
     CDS             complement(2637..3293)
                     /locus_tag="SDD03_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4305034.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3303..4580)
                     /locus_tag="SDD03_002627"
     CDS             complement(3303..4580)
                     /locus_tag="SDD03_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4305035.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4599..>4754)
                     /locus_tag="SDD03_002628"
     CDS             complement(4599..>4754)
                     /locus_tag="SDD03_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG4305036.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1487 a          866 c          571 g         1830 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt
       61 ttttgtgact tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat
      121 ttttctaatg gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca
      181 tacatctact gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg
      241 tgcagcgttt aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata
      301 atccttactt gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac
      361 aatgacagaa ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga
      421 aatgaaaaaa acatcttcta caccacctac tttcagttca tcaaatactt gcatccagcg
      481 atttttagat tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc
      541 atagccaaga atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac
      601 gtacatacaa tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc
      661 ttccaattca ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa
      721 gccgtaaata tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat
      781 ggaaagaacc ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg
      841 ttcaaaagaa gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt
      901 aagcgtctta ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg
      961 ggcgtcatac cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc
     1021 aaacacatct tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa
     1081 aatggattca gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc
     1141 acctcttgat cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga
     1201 tggttactta cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt
     1261 ttaattattg tcaattaata attcttttgg attccttctt aatatattga atgaagaaat
     1321 aatcaatgat acaagaagta ctaaactcat aaatataact acataaatca tgaatttagg
     1381 taatacattg atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc
     1441 acctaattgg ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt
     1501 attcaatata ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac
     1561 gaatgcgggg attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt
     1621 tgatatacct aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa
     1681 taataataat gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga
     1741 aattgagtaa atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat
     1801 caaattatat tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag
     1861 attcttatca ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt
     1921 accataaact ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg
     1981 tgctgcactt actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac
     2041 tgtttcatct gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt
     2101 atctccaact ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt
     2161 atcttcattt tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt
     2221 ttcttttgtt gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct
     2281 ttcaggtgat tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc
     2341 tgcaacactg tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt
     2401 aatatcttca ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt
     2461 tatctccata gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt
     2521 agcatcttta atagataaac taatgagact taaagttgac atagataaaa taactaatat
     2581 gataattaat gattttaaac tttttctagt tacataagca aatgcatttt ttatcattat
     2641 tcaacctcca aattcatttt atttactttt ttcaactttt taccacttag ttctaaaatg
     2701 atatccgcag aatctgctac ttccttacta tgtgttacaa ctattacaca tttatttcta
     2761 tctttagcta atgtctttaa tatattaatt atttctccag cagtaacact gtctagatta
     2821 ccagtaggct catcagctag tattattgga gcatctgata ccaatgctct agcaatagct
     2881 actctttgtt gctgtccacc agataatttc ataacatttc tttttatttg ttttttatct
     2941 aaacctagtt caaacaagat actttcatct gctgatttat ttactaatct aatattttca
     3001 attggtgata aataatctat taaattatag ttttgaaata ctaaagatat attatttttt
     3061 ctatgattac tatatccttt attttgtata tcttcattct taaacaatat ttttcctgtt
     3121 tgaggtttat caagtccagc aagtaaggaa agaagtgtag attttcctgt tcctgacttt
     3181 cctactatcg cataaaactt cccaagttca aatttttgat ttactcctga caaaactttt
     3241 tctttagaat ttgcataact atatgctaca ttctttattt ctaatatatc cattattttc
     3301 tcctaactta tttttgataa gatttcttta ggcttcttga ttaacattaa tgaagaagca
     3361 aatacaactg ataaaacaat aatacttatt aatattaaat aactttgtcc aagtgttgtt
     3421 atatttgaca taaaactact attatttatt aaacttccac cggaaatcat tgaatcctct
     3481 gagttaatga atccatctac aattactttt agtaatacat ttcctaaaaa taaggaagat
     3541 attatacttg gtatcgatat gaatattaac tcgaatataa attgcattat aatttgtatc
     3601 ttagatgttc caatagataa gaatatacct atttcataaa ttctttccct taaccataga
     3661 atcaagatta atgaaagaac aaccatccca cctaacataa tagaataagt cattactttt
     3721 attatatatt ttattccact cactgactct aaagactctt caaacgcatt agaatctttt
     3781 tcaacaaaat actttgactc atcaatttta agctctttca atttgtttaa ggctaagtct
     3841 gtagattctg cactaccaga atacattaaa attttatttg caatttcatt attttctgaa
     3901 ttatttaata tctcttggct tgttgaataa tctacaaaca ccatattttc actaaaatca
     3961 gaagataatc ctgtatatgt ttcctgtttt ttaccagaaa agatccctat aattttaaat
     4021 ttatgacttt ttatttttcc acttttttca atatctagta attcaagatc aacttcatca
     4081 cctaatttta gattgttttg tttagcaaat tcttcatgaa caataattga attcttatca
     4141 ttttcttcta tatttttacc ttctttaatt gtaaatactc cactactaaa taaaatattt
     4201 cttttagtat tatttgtagc ttcgagtgaa acaacattct taaattcgtc agataaatct
     4261 tctctattta ttctttgttc tccgctaact actttagcat cttttagttt tgctaatcca
     4321 tcatattgaa ttattttttc ttcaatttct tttaattttt caatatcttt aaattgatta
     4381 acattaaaat atttaccatc tttttttgtt attgatattg aagaattaga actttcatat
     4441 aaagcctttt ctatttcatt gcttgatttc attattgtta aacaagaata caagcaagaa
     4501 agaactatag ttaatatgat aaaaattatt aaagttctat ttttctttct tgtaatatat
     4561 gctatagcat tttttatcac tttcctaact actcctctct attttcttac tccataacta
     4621 attgcatcca ctggacaagc atttttacat cgtccacatc tgatacactc cagatgattg
     4681 caattctcaa ctgaatcttt attcatttgg caaacttttt tacattttcc acaattaata
     4741 cacttatcct tatc
//
LOCUS       DARDHU010000140         4729 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000140 DARDHU010000000
VERSION     DARDHU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.143"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            111..920
                     /locus_tag="SDD03_002629"
     CDS             111..920
                     /locus_tag="SDD03_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4305037.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            960..1457
                     /locus_tag="SDD03_002630"
     CDS             960..1457
                     /locus_tag="SDD03_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4305038.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1842..1915)
                     /locus_tag="SDD03_002631"
     tRNA            complement(1842..1915)
                     /locus_tag="SDD03_002631"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1879..1881),aa:Ile,seq:cat)
     gene            2102..2425
                     /locus_tag="SDD03_002632"
     CDS             2102..2425
                     /locus_tag="SDD03_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305039.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2589..2843
                     /locus_tag="SDD03_002633"
     CDS             2589..2843
                     /locus_tag="SDD03_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305040.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            2913..3158
                     /locus_tag="SDD03_002634"
     CDS             2913..3158
                     /locus_tag="SDD03_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4305041.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            3234..3599
                     /locus_tag="SDD03_002635"
     CDS             3234..3599
                     /locus_tag="SDD03_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305042.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(3660..4223)
                     /locus_tag="SDD03_002636"
     CDS             complement(3660..4223)
                     /locus_tag="SDD03_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305043.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         1501 a          832 c          792 g         1604 t
ORIGIN      
        1 ttttgaaaaa attcataatg acaaagcaag ttagtttcat ttttttaaat tctcatttaa
       61 tcttaaggta acaggaaaat agatatgtaa aaagaaagta ggaagataaa atgaaactag
      121 cagcgattga tttagacggt accttgcttg atcgaaatgg aaagatacca gaagaaaata
      181 tccaagcact tcggaaattt gatgaaaatg gcggaatcgt aaccgtcgct accggaagga
      241 acagcatttc tgctaaagat attttttccg aattaggaat cggtggctat ttgatttctt
      301 ccaatggttc actgattgct gaaatgaagg atggaaaaat ggcgaaagtt ctcagacgtt
      361 caaaaataga tagcgataca ctgagaaaag ccttcttcta tgctcaagaa gcgaaaatat
      421 ccatcattgc tagccgagaa acccaagatg atcagctgac attcgatgaa tcaaatctag
      481 tcaaagatga cccttattat gaacatttca atttacagaa ccattctttt gatcaaattt
      541 taaatatgtt ggaagatcct tctcttagct atcttaaatt agcattaaca gataagagtg
      601 aagaaaaact acagaagcta caaagagatc tgcaagcaga tggtatccaa tcagtattct
      661 ctgatccgca tttcttagaa atcacgccta caggtatcac taaagctcat tcacttgttt
      721 ttctggcgga ttaccttgga atcgaatcga gtgacatcat ggcttttggc gatcaagaaa
      781 acgacattgc tatgttgaat tacagcgggt tgagtattgc gatggggaat gcacagcgac
      841 aagtaaaaga aattgcaaac caagtaacac tgacgaatga agaagctggt gttgcttata
      901 ttttaaaaaa cttctattaa aaagaataaa atgacgatat gaatgtagga ggaaagaaaa
      961 tgttagttat tattgatatg caaaatcata ttcttgatcc aaacagcgaa ttttatattc
     1021 cagagtcaga tcaattggca gaacgtattg ctcaaagatt agctaaagca cgccaaaata
     1081 atgaatatat tctcttcaca cgggatatac cagttgaact gaaggacaaa gaagaagaac
     1141 gagaagattt gcaattgatc cctcttcttt ctccacaaga aaatgaacga gtgattaaaa
     1201 agtattactt tacgattcca cctgaaactt tagctgaaat ccaggaaact ttatttgaaa
     1261 gcaaagaaga acaaaaagag atcgaagtag tcgggattga aacaaacttg tgtgtactct
     1321 ctaatctgat aggcctgcag agtgcttttc ctgaagcaga cttttttgtt gatccaatgt
     1381 tagtctccag tagaaaacac ggagaatccg ctttagagct gttgaaagat ttcaacgtct
     1441 ctatcgtcga atcataaaaa tatctatctt catttacgat atttaacaca caataaaaaa
     1501 agcacgcaga actgttttag tcctacgtgc tttttctatc aaagtagatt ggctttttta
     1561 tgacacagag tgaaacggca ggctcacgac ctctttctta aatggtgttc aatcagctga
     1621 ccctcggtat tagcttaaca aacggcaaaa tatgaggaac tatttttccg tttgttaagc
     1681 taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtat tcaaaagctt
     1741 gcatctgcag taataagaac agccaagtaa aaacataaga agctgttttt acttggctgt
     1801 tcttattact tgatgcaggg cagctttttc acaacctctt atgtaccttg taggactcga
     1861 acctacgacc ggacggttat gagccgtctg ctctaaccaa ctgagctaaa ggtacatcat
     1921 aaataaaatt tatttttgtt acttttcaac tatagcacga atgacaaact ctcacaagtt
     1981 tcaaaatata agtaaaataa tattgttatt cttatttctt ttacttgtgt cataactatt
     2041 ttgttacttt aatttttatt tgcttatttt agaaaaaatg ttattctttt atttattctc
     2101 aatgttagga gtgattattt tgccatttat tgcgatttta ttcgatttag ttgcggcagc
     2161 agcttatttt ctccaattaa attatcaaac ctcaggaatt ctgtttattg gaatgatttt
     2221 tcaaggtgtg attacccttt tgcttcttgt catgatgctc agctataaag gaaaaagata
     2281 tgcccgtatt caaacaaaag tatttgttaa atatgtttcc attcgctatg ggatcatcat
     2341 tctttccttt ttagtgaatg cgatcgtcct ctttttatat gtattgaatt acttagatat
     2401 caatccattg attttttctc gatagataag acacattcag cggaacagtt cctgcttatc
     2461 agcagaaaac tgttctgctt tttcattttt cattgatcta ttcttctttt attttcctag
     2521 catttcattt gtgatgtatg caataacatg ttatctttta ttatatgaat cgttgaggag
     2581 tccgttacat gattacgttg gtgagagttt tgttttgggt gcctgcggtc gcactggtcg
     2641 caagtattgt ttatcttatg aattggaaca aagaacggtt ttatttagct attttgactc
     2701 ttccggctat ctactttatg tggaaagttt ttaattacaa ctactttgaa cccgatagtg
     2761 tttttataga agaattatca ggactggttt tgtctttatt gatcgtcatt ctttatttga
     2821 tacgtttaaa taaaaaacat tgagtactat tgattgcatg tgtagctttc tatagtcatt
     2881 ataactattc tactataatg aaaggtgatt aaatgagtat cgaagaagct attttactgg
     2941 atactttacg ttgtcgtctg aatttcttaa gaactggccg atctttatct caagtagctg
     3001 tttctagcgc gttaagtatt cctttattca aatatcgtaa gtacgaagaa ggagaagaaa
     3061 ttccaagcat cattgaatta atctatatcg ctgactttta cgatatttcc gttgattacc
     3121 taattggtcg ctcagaaaca gcaagcaaac tgaagtgatt ttttccagcc ttggtgctgg
     3181 ttttttcatt taacgtctaa ggttaacaat ctatatataa aggagacatt tctatgcgat
     3241 tatggcatga aaaattgatt tcaaagcttc ctcgtcaaca gcttttagga caacatcggg
     3301 aatgctgtgc attaagagga aatggctggg gaaagaaaca tgctactgtg gattatgttt
     3361 ttcactattc tccttataga ttattccaat atcatcagct tgtcatggat gaaatggaaa
     3421 aacgtggtta cagcccagca ccagagtgga aaaatcctat gtatcgtggc aaatcatgcg
     3481 aagcattttt tgatttacct actgtagagc gcaacgtgcc gatttatccg gaacacgatg
     3541 aagtttatct agcagaatgt ttagcaaatt tggagcaaaa aggaatttat cttacctaat
     3601 ataactagaa aagccttcta tctcctctta cttcattaaa aagctttaac catcttagat
     3661 caagctaaaa tgtcgtaagt tttagggtta tcttcatcct cacgtgcaaa actaaaagcg
     3721 tactctagtt ttggttcatc gccaacttcc ctagacataa atgaacatct cctgacgtgg
     3781 tttaaaagta ccgcatcttc gacgtctgta tacgatgcac agcacagatt gcttcttttg
     3841 atctttcttt tgatttttct gttgcttctt ctacaccttc ttcgattact acatcgatgc
     3901 ttacaattgc ttctgcctaa tattcccatg catcgtcgat ttaagcctct gtagtcactg
     3961 tgatcgtttc cacattttaa ctcctctgtc gtggcttcag ttgattttgt gatcagttca
     4021 gttgctaagc cagagtcaga acaacctgcc attgctagcg ttaatattga tagtaaaaat
     4081 agttttttta tcataaggtt ccccccctga aaatagacta aaataataac gctttctttt
     4141 tttgttttca gacgaaaatt aataatttct aaatattctt taaaaaagct tataactatt
     4201 atgccttttt taatatataa cacagttatt tttaaaaata agaatgctaa atcactctac
     4261 aataacggcg aaagcacaaa aaataaaaaa attattacgt tattaaaaaa acagatgaaa
     4321 gatcaggttt tattagatct cattctttca tcacttgaag ggatttaatg aactactttt
     4381 tcctctttgt cgtttttata tattcgtatt ccttcaaaca gtaggatagc ctctttcaat
     4441 gcttgatatt tgaactgtcc gttacttaaa aatttagatg tgccgccctc ttcctagaac
     4501 atattctttt ttactttttc attaattgac agaccaatcg aacgaaactg taaatttcga
     4561 taggataatg attggtcaaa ctcattcatt ttcaagacat ctcgattcat attcaagata
     4621 ggaatctttt tatcaacatc actttattcg tattttcctc taccataaaa gcttcacgct
     4681 ccacaaaatt gttttataca ctactattca gcccattcaa cgaccaatg
//
LOCUS       DARDHU010000141         4711 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000141 DARDHU010000000
VERSION     DARDHU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.144"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            106..357
                     /locus_tag="SDD03_002637"
                     /pseudo
     CDS             106..357
                     /locus_tag="SDD03_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="SDD03_002638"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="SDD03_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG4305044.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="SDD03_002639"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="SDD03_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="SDD03_002640"
     CDS             1786..1869
                     /locus_tag="SDD03_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG4305045.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="SDD03_002641"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="SDD03_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG4305046.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="SDD03_002642"
     CDS             complement(2994..3857)
                     /locus_tag="SDD03_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEG4305047.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="SDD03_002643"
     CDS             complement(3859..4131)
                     /locus_tag="SDD03_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4305048.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="SDD03_002644"
     CDS             complement(4148..4357)
                     /locus_tag="SDD03_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG4305049.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="SDD03_002645"
     CDS             complement(4455..>4711)
                     /locus_tag="SDD03_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEG4305050.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DARDHU010000142         4646 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000142 DARDHU010000000
VERSION     DARDHU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.146"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..717)
                     /locus_tag="SDD03_002646"
     CDS             complement(133..717)
                     /locus_tag="SDD03_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4305051.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="SDD03_002647"
     CDS             complement(928..1131)
                     /locus_tag="SDD03_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305052.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="SDD03_002648"
     CDS             complement(1141..1563)
                     /locus_tag="SDD03_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305053.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="SDD03_002649"
     CDS             1801..2415
                     /locus_tag="SDD03_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4305054.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="SDD03_002650"
     CDS             complement(2580..3404)
                     /locus_tag="SDD03_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305055.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="SDD03_002651"
     CDS             complement(3428..3583)
                     /locus_tag="SDD03_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305056.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="SDD03_002652"
     CDS             complement(4042..4299)
                     /locus_tag="SDD03_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4305057.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="SDD03_002653"
     CDS             complement(4292..4561)
                     /locus_tag="SDD03_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4305058.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DARDHU010000143         4634 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000143 DARDHU010000000
VERSION     DARDHU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.147"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            174..518
                     /locus_tag="SDD03_002654"
     CDS             174..518
                     /locus_tag="SDD03_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG4305059.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="SDD03_002655"
     CDS             519..938
                     /locus_tag="SDD03_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG4305060.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="SDD03_002656"
     CDS             complement(1030..1380)
                     /locus_tag="SDD03_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305061.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="SDD03_002657"
     CDS             complement(1346..2566)
                     /locus_tag="SDD03_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG4305062.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="SDD03_002658"
     CDS             2932..3498
                     /locus_tag="SDD03_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4305063.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="SDD03_002659"
     tRNA            3643..3730
                     /locus_tag="SDD03_002659"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="SDD03_002660"
     CDS             4064..>4634
                     /locus_tag="SDD03_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG4305064.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DARDHU010000144         4608 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000144 DARDHU010000000
VERSION     DARDHU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.148"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(83..337)
                     /locus_tag="SDD03_002661"
     CDS             complement(83..337)
                     /locus_tag="SDD03_002661"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4305065.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(448..702)
                     /locus_tag="SDD03_002662"
     CDS             complement(448..702)
                     /locus_tag="SDD03_002662"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305066.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(895..1170)
                     /locus_tag="SDD03_002663"
     CDS             complement(895..1170)
                     /locus_tag="SDD03_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG4305067.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1142..2095)
                     /locus_tag="SDD03_002664"
     CDS             complement(1142..2095)
                     /locus_tag="SDD03_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4305068.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2707..4200
                     /locus_tag="SDD03_002665"
     CDS             2707..4200
                     /locus_tag="SDD03_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG4305069.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4335..>4608
                     /locus_tag="SDD03_002666"
     CDS             4335..>4608
                     /locus_tag="SDD03_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG4305070.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          801 g         1541 t
ORIGIN      
        1 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
       61 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      841 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      901 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      961 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
     1021 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
     1081 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1141 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1201 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1261 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1321 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1381 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1441 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1501 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1561 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1621 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1681 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1741 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1801 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1861 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1921 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1981 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     2041 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     2101 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2161 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2221 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2281 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2341 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2401 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2461 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2521 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2581 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2641 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2701 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2761 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2821 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2881 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2941 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     3001 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     3061 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3121 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3181 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3241 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3301 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3361 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3421 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3481 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3541 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3601 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3661 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3721 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3781 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3841 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3901 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3961 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     4021 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     4081 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4141 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4201 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4261 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4321 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4381 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4441 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4501 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4561 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaa
//
LOCUS       DARDHU010000145         4475 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000145 DARDHU010000000
VERSION     DARDHU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.149"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            323..1054
                     /locus_tag="SDD03_002667"
     CDS             323..1054
                     /locus_tag="SDD03_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4305071.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="SDD03_002668"
     CDS             1199..3226
                     /locus_tag="SDD03_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG4305072.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="SDD03_002669"
     CDS             3334..4431
                     /locus_tag="SDD03_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG4305073.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DARDHU010000146         4326 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000146 DARDHU010000000
VERSION     DARDHU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.150"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..283)
                     /locus_tag="SDD03_002670"
     CDS             complement(<1..283)
                     /locus_tag="SDD03_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4305074.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="SDD03_002671"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="SDD03_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="SDD03_002672"
     CDS             complement(2361..2723)
                     /locus_tag="SDD03_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305075.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3065)
                     /locus_tag="SDD03_002673"
     CDS             complement(2727..3065)
                     /locus_tag="SDD03_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305076.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3044..3463)
                     /locus_tag="SDD03_002674"
     CDS             complement(3044..3463)
                     /locus_tag="SDD03_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305077.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="SDD03_002675"
     CDS             complement(3456..3689)
                     /locus_tag="SDD03_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305078.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4326)
                     /locus_tag="SDD03_002676"
     CDS             complement(3686..>4326)
                     /locus_tag="SDD03_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4305079.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1224 a          962 c          620 g         1520 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggat
//
LOCUS       DARDHU010000147         4282 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000147 DARDHU010000000
VERSION     DARDHU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.151"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..1257)
                     /locus_tag="SDD03_002677"
     CDS             complement(37..1257)
                     /locus_tag="SDD03_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG4305080.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="SDD03_002678"
     CDS             complement(1277..1888)
                     /locus_tag="SDD03_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4305081.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="SDD03_002679"
     CDS             complement(1931..2863)
                     /locus_tag="SDD03_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG4305082.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="SDD03_002680"
     CDS             complement(3284..4144)
                     /locus_tag="SDD03_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4305083.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DARDHU010000148         4253 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000148 DARDHU010000000
VERSION     DARDHU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.152"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            56..1357
                     /locus_tag="SDD03_002681"
     CDS             56..1357
                     /locus_tag="SDD03_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4305084.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1384..1644
                     /locus_tag="SDD03_002682"
     CDS             1384..1644
                     /locus_tag="SDD03_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG4305085.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2643..3878)
                     /locus_tag="SDD03_002683"
     CDS             complement(2643..3878)
                     /locus_tag="SDD03_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG4305086.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1229 a          683 c          880 g         1461 t
ORIGIN      
        1 ctttgcgttg tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga
       61 aaagctgatg aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca
      121 gcgtcattta ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg
      181 gacatttttt atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc
      241 ttattcggca acgattgttt taccctttag gatgacgatg ggattgatgg ctatttatgc
      301 aacttttgtt atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg
      361 tatattatct ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga
      421 agaggcacct cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc
      481 ggggatctta tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat
      541 tactatcaaa atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat
      601 tccaggtgca gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt
      661 aaacgctgcc ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct
      721 tggtgtttta ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat
      781 gagtattgta ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa
      841 ggcattggca gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta
      901 tcaatggacg gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat
      961 attcttctgt aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat
     1021 cttcaatatc aatgagccca taatttttgg agctcctatg atgttgaatc cttacatgtt
     1081 catcccgttc aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt
     1141 agaaatggtc aatagattta ctgttttgcc aacatggaca ttaccagctc caatcggcgg
     1201 ttatctttca gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat
     1261 agcatttatt atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga
     1321 acaaagtcgt ggagaaaacg aagtccagac tgtctaagtt tggtttttag aaggtgggaa
     1381 acaatgtatt ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt
     1441 atattgaata tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca
     1501 tcatttttga tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca
     1561 tgggggacat atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga
     1621 tggtttgtca catcgaagct ttaaatgttc taaaagtggt tgtaacagaa gtggttagct
     1681 tcaagaaata agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt
     1741 atttttcgtt tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg
     1801 aatatgggac tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt
     1861 tttaactgtt ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc aatggattta
     1921 gaattagtag atctactacc aaagtgatca agtcaaatat agatttggga gcttacaacc
     1981 atatagtagg ggctgcagta tggtgtatga atggtaatat gtatgatgtg agtgtttatt
     2041 caaatcatga aacgtgtata gaaataattg tgctgggaat agcaatcagt catggtgaac
     2101 gtgactttta gtgtatcgtt gctgacgaca tagaacattc tgacatgatc tactcaatat
     2161 gtggggattg ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa
     2221 cagaagaggg aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat
     2281 tgttgagggg actagcggca caaatctcta caaagcgatg cttctagatc tatatgacac
     2341 attttaagaa aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat
     2401 gatccaacca gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta
     2461 tgctcatttg gatagcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac
     2521 cgtaatttaa gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt
     2581 ctgggtctcg aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga
     2641 aactatattg ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca
     2701 tttattggaa aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact
     2761 tcgcgcagag caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt
     2821 ccgtatttta cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata
     2881 aacggtttgc cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa
     2941 aaatcaatga attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct
     3001 gagtcagatt gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt
     3061 tgtttttgta agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt
     3121 gcgaaataga taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct
     3181 tcaggcaagg aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc
     3241 agctgttctt cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc
     3301 atttgtaaac ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca
     3361 ttggggatcc atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt
     3421 aaagggccga agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt
     3481 tcactcaaag catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa
     3541 aaaattgtca cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc
     3601 tgttcactag tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc
     3661 aactggtcaa attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa
     3721 tgatactcaa agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat
     3781 acacagtaat aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct
     3841 aaattgaata ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga
     3901 gtctatccct tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta
     3961 tattttgaat attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa
     4021 tcaagttgaa actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatgt
     4081 tgtgccagat aaatacgaga agttgtgtca ttaaaaataa acatgagaac ataaaaaaat
     4141 aaaataatta tattaattga taggtgtgct gtcgtcatta tacacacgta agaaagaaat
     4201 agttatgttt aaaaatattc ttagactttc tcgccatgac aattttcact atg
//
LOCUS       DARDHU010000149         4198 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000149 DARDHU010000000
VERSION     DARDHU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.153"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            66..302
                     /locus_tag="SDD03_002684"
     CDS             66..302
                     /locus_tag="SDD03_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG4305087.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            321..536
                     /locus_tag="SDD03_002685"
     CDS             321..536
                     /locus_tag="SDD03_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG4305088.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1239..1991)
                     /locus_tag="SDD03_002686"
     CDS             complement(1239..1991)
                     /locus_tag="SDD03_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4305089.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2006..3253)
                     /locus_tag="SDD03_002687"
     CDS             complement(2006..3253)
                     /locus_tag="SDD03_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4305090.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(3329..3475)
                     /locus_tag="SDD03_002688"
     CDS             complement(3329..3475)
                     /locus_tag="SDD03_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEG4305091.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(3579..3890)
                     /locus_tag="SDD03_002689"
     CDS             complement(3579..3890)
                     /locus_tag="SDD03_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4305092.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(3892..4089)
                     /locus_tag="SDD03_002690"
     CDS             complement(3892..4089)
                     /locus_tag="SDD03_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG4305093.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         1429 a          676 c          587 g         1506 t
ORIGIN      
        1 gatttttact gttaatcaaa tacgtatgtt acagtagcac cgtaaaaaat attatttaaa
       61 gaagcgtgtt taaacatgca aaatgtaaag gaactaagta ttgtggaaat gcacaaactg
      121 ttggtggagg caataatgct tggggaaaat tggggcaagt tgtaggtgga cttaccacag
      181 gggcagtagg tggtgcaggt ttaggaacag caatttgcgg accagcttgc ggtgttgttg
      241 gagggttata tggagctgtc gctggaggtg ctgcagctgg ctgggatgct cgaaaaaaat
      301 aaaaaaggag aataagtagt atgcatatta aaaacacaaa aacaacattt atattatctt
      361 cagaagagtt gaaaaatatt caaggtggga gtgccgtggg agttttaggc actacttttt
      421 ctggggcaac agcaggagta aaactttgtt cagccggagg accttatgca atagctgctt
      481 gtggtgtagg tggagcttta ttaggtgctg gattcagcat gtggaccggt gcgtaaacaa
      541 ttaaaaaata tcaaacctca agattggttt gtgtcgatta cgaacatagg tatgttcttt
      601 atcttttcaa aagtttggaa tggacagaac tttttactaa agcttcttgt gctagttttt
      661 ataattatat taaattttta tcttttatta aagtcagata aaaattcttt ggaaatgtaa
      721 tggtagagat atttttacaa aaggacttgc agcgctgtag ttggtgctgc aggaggacct
      781 atttcggccg ttacaggagg tattggtaat gtacttgtcg gatgtgcatt aaagcctgtt
      841 cctgcaagtt aaatttataa aaacaggaaa gtaattatct caaatcctat caagtacaat
      901 cataggattg cacttgacag gacaaatttt ttaactaaaa aatgaaaaac actttctaaa
      961 aattagtaga atttgccaaa tctttcgcat cttccaccat acatttaatt ttaattcccc
     1021 tttatggtac ttaataaaaa caagaaaaga actattcatt tggccctttt ttggttccag
     1081 accatagaaa tattcctcca gtaactaata ttgctgttat gcacgtaact aataacagct
     1141 ataaaaattg gtacaacttt aataggattc agaaaaattt gcactagtga aggaatctat
     1201 tttattagaa aaatgcaatc tccctaaaga aaaaaccact aaacagtgct ttctttcaac
     1261 ttctccttaa ttttttgtgc tttccctaaa gtaaagtttc tggccaactc tgaatcaaaa
     1321 tatagtgaac gttttttaaa atctatttct ttaacgttaa agatatttac taaacacgct
     1381 cgactaattc ttgttaacat aggatatttc ttttcaagct cactaattct tccataaaat
     1441 tcgtactgcc catctttagt acacaatgat aatctatgtg gtaattttga agcttctaca
     1501 aaataaatgt catttatatc aaaagtaaat gtttgagaac ctatggaaaa aacaaatgct
     1561 tgattcgtaa cctgttttgt tgcatctatc ctttcttgcg ctaataacaa taactcaaca
     1621 atctcgtttc gatactcatc tagtgtttga tcttttgtta caaatcctaa cgtttcaact
     1681 cttcttttta ttgttacggg taacatctca tcatgagtag ttgtaaaaat gatttttgct
     1741 tgaacatcat attttctgat tacttctgct aattctatac cgttaacttc atgatttaaa
     1801 tcaatatcta agaaatatat tccttgcttt ggtcggaatt gtttgaggta ttttttaact
     1861 tctaacggac tttgtgtttt caatactatc ttaaatactt tatcgtgaaa aagaatgaaa
     1921 ttatcaataa ttcgttctaa ctgatttaat tgtataattt gatcttcaca taatattatt
     1981 ggataactca taaatcctcc ttccctcaaa acaacaaaat aacttgagta gtaaattgat
     2041 acggatcgtt ttgatagttc acaaacatat tgggattctt tttattgatt tcctctatac
     2101 tagataaacc taacccttga tgtccctttt ttgttgttat tccttttttc attagagtat
     2161 ctatagagag tagtatctct ctacaactat tcgatatcga aatttctatc tgtttatcat
     2221 ctcggtaaat agctagagac agttctctat cttgactctc tatagatgct tcaattgcat
     2281 tatcgagaat aattccaagc accctaatac agtcaaatat tggaacagga acttcaataa
     2341 tagtacttgg acattcgaat ttacaacaaa tcttattgtt atttgcttgg tatagcttcg
     2401 ctatcaacaa acttttcaaa tattcatttt ttatattttt taaataacga tattcaaact
     2461 tattggttag ataagaatta gaatagatct ctaattcttt tatttctttt tccaatatcg
     2521 aatcatggtt ttgattagcg atttctttta aactcagcaa taaattttta tagtcatgtc
     2581 taaatctggc aaatttttct tgatcttttt ccagtgaatc agtatatttt tttagataaa
     2641 ccaattcttt tcttttcaat tgctgttcat attgttctcg ttgcctaata gttattctaa
     2701 taaataaaaa tagaacaaca aacatttgaa tgattaaaaa tattaagaca ccaaacacaa
     2761 aatgatcaaa tacttgatat tgatgagcaa cataagaaac aaataaacca attgtaagta
     2821 aataaatcat gaatatagca gtaagtttag aactggactg ttcaacaatc ctatttatag
     2881 ctaattttct atataaccaa ataaacatca gcaaaaacaa agaatcaagc attatctgga
     2941 atactacata accataccct tttatccctt caaatgaaaa catatacgtc ataaccatgg
     3001 aagtaataat agatctgaaa tcatcaataa gcatacataa tagtaattta tttaaaagca
     3061 ctatattagt tttcttcttt tcctttaata taaaataact tccgattata ggtatagctg
     3121 cggaaacatc agtaaataat tcaacaaaaa aagtgagtgg tatcaataaa catacaaaaa
     3181 gatattttct tttgttaaga tcagtattag tcatataaaa taagagaaaa aagaaaaagt
     3241 ttcccacgaa cattcttaat agataagctt ttgtatcgat agtaatcaag agatcccctc
     3301 ctgattttag gatatcataa aaaaaatttt agttacactt tttaaataat gaaaaaacac
     3361 attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct cctttaattt
     3421 ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct tccattacga
     3481 ttccccctac aaactataaa tacttttatg tcactgattc aatataccta tttttctaat
     3541 caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga tttatctcca
     3601 taatctgctc gatataaacc atttattcca gcagttcttc caatttcact caaatttctt
     3661 attaattcct cttgatattc agtgaatctt attttattag tataagcagt aaaatataga
     3721 taattcacca atctatttat taaaggaccg ggatctaatt catatttttg tttttctaat
     3781 tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc tttacttata
     3841 gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat tttagcactt
     3901 ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac aagtagttgc
     3961 cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc cataatattt
     4021 tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca aaatttttaa
     4081 atgtttcata atctaatcac ccctaaatta atgtttttag actattattc taacattcat
     4141 taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga ataagaat
//
LOCUS       DARDHU010000150         4128 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000150 DARDHU010000000
VERSION     DARDHU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.154"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..734
                     /locus_tag="SDD03_002691"
     CDS             <1..734
                     /locus_tag="SDD03_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG4305094.1"
                     /translation="KTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENM
                     VFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENEL
                     AVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYF
                     SVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSR
                     VFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            751..1185
                     /locus_tag="SDD03_002692"
     CDS             751..1185
                     /locus_tag="SDD03_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305095.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1802..>4128
                     /gene="esp"
                     /locus_tag="SDD03_002693"
     CDS             1802..>4128
                     /gene="esp"
                     /locus_tag="SDD03_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG4305096.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1518 a          575 c          781 g         1254 t
ORIGIN      
        1 gtaaaactat cgagcaaatc aaaatagata atctgttatc acaaaattac gatccagaaa
       61 tatatctgtt tctttatgaa aataaaatac tggaatatgt taaaaatgga gatattcgaa
      121 atttagaaaa catggttttt aatctgagta atggaataat tccaagtgtt agtggagata
      181 cgattcggtc tgaaaaaaat tattctatca ttgtttttga aaagttagca caaacgtcta
      241 taacattagg aatggatata atagaagcgt atcaaagtag agatgctttg atacaagaaa
      301 acgaattagc tgtatcgtta cctgaggtct taaaagtgag ggattctggg attgtttatt
      361 atactaagga aatcggaaaa actaagatag agcatctttc tcccttaatt tcttctgtcg
      421 ttcaattcat cggcttaaat atctataaaa gaattactgt aaaggaaata gctaattatt
      481 tttctgtaag tgaaactaaa ttaagagaaa gctttaagaa tgaaatgcat attacgattt
      541 ataattatat ctctaagcga aaaatttcta cggctaaaat aatgttgaaa tcaaaccata
      601 ctatcagtga agtttcttta ggtttaggat tttcagattc ttcacacttt tcgagagtat
      661 tcaaaaagta cgcaggtgta tctcctaagc aatatcaatt aggtttagtg gacaatttca
      721 acaacattag ctgagaggtt ttattttatt atgagactat atcatttacg tagtttggag
      781 gagttgagga aattggattt taacaaaaaa tttttttggg aaatttctga tttgagttgt
      841 ttcgtagaat ctgagcgtct aagtaatggt aataatagtg tgttaaatga atatgacaaa
      901 gtagtaatta ataaagtaaa aagtttatgt gaagaagcta gagaatctat cagtcatatt
      961 aatggtagca attttaaaca aacattattt aatctgatta agctagatgc taaaatatca
     1021 agttatttat tctttttgat acatgatgaa tttatggatt atcaaaaatt ggatcagtta
     1081 attgaaagag aaagctggga agattattac gtgggtctaa ctttttcaga aaagttaaac
     1141 aactacaagt taatagatta taagtatttg agtgaaagca attaattatt catgtagagt
     1201 actagacact ttaaatatgg acaattcaat tataaagaaa aagaatcaat aatttaatga
     1261 ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg ttgataactc
     1321 caatcattcg tttaagattc taggaatcat tcagaaatgc tgtgaaaccg atactttata
     1381 attataaaga aggtgatttc aaattccgaa gaaggaattt gaagtcacct ttttacttat
     1441 gtcacagtct cattcagtaa tgcgggataa ggctccttat tccgttattt gaatactctg
     1501 aagtttaatc taagaaaggg taatattttg aaaacatgct tcatcgcata gaaatgaact
     1561 taatttcaga aagaatacat acatatcttt ttatatgttt tcagctgctg aatggttaaa
     1621 aagcacaaat atccgaaaaa aacatgaaga aatgtacttt ttttgttgta atgttcatga
     1681 aattctctaa gtaaattagt taacataagg ttagtaagat tcctattctg tgaataatac
     1741 gtatcagatt tatcttttat tcttactagg aaagtaatta ttaagggagt tgatttgaat
     1801 aatggttagc aagaataata agagagtatt tctagaaaaa acaaagaaac gagtattgaa
     1861 gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt ttgattggtg ttggtcttgt
     1921 cttgggaaca actgaactag ttaaagcaca agatgaaatt tctccaagca gcccacttga
     1981 aacagcgata agctccgtac aagtaggtga caaagtagct tcggggaata cttttcagga
     2041 gaatcctggt tatacgaaaa attataactt ttcggattta caatttagtc ctcaagaact
     2101 aactggagac acactaaaag gcaatacaat tggatttgaa gtttacggaa aacataatat
     2161 tgcagcatca actaaaaact gggaaatccg tcttcaatta gatgaacgat tagctaaata
     2221 tgtagaaaaa attcaggtcg atccaaagaa aggaattgga tcttctagac ggacctttgt
     2281 aagaattaat gattcgctgg gtagacctac aaatatttgg aaggtcaatt atattcgtgc
     2341 aagtgatgga ttatttgctg gggcagaaac aaccgatacg caaaccgctc ctaatggggt
     2401 aattacgttt gaaaagagcc tagatgaaat ttttaaagag ataggcatag acaatctcaa
     2461 gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat caagacgatg ataaaattgt
     2521 accgggtatt gatagtacgg gctacttttt aacagattca gatgatttct ataatagctt
     2581 agatgtatct gaaaataatc ctgaccagtt taaacatgga tcagtaagtg caaaatatga
     2641 gaaacctaat actcaaacaa aagatggttc aggatccaca ggtgctaatg gagctataat
     2701 cttagatcat aagttaacga aaaattataa tttctcatat tcatcttccg caaagggcac
     2761 gccttggtat gctaattaca aaattgatga acgattagtt ccatatgtag cgggtataca
     2821 gatgcacatg gttcaggctg acaaagtaac atatgatgtt tcgtttgaat ccggaacaaa
     2881 agtagctgat ttagcaatcg agcgacggga aggtcatgaa aattatggca taggttcaat
     2941 aactaataat gatttaacta aacttatcga ttttgctaat gcaagtccac gtccagtcgt
     3001 tattcgatat gttttacaat tgactaagcc attagatgaa atcttagaag atatgaaagc
     3061 aacagcacaa gttgaagaaa ataaaccatt tggtgaagat ttcatctttg attcttggtt
     3121 gtcggatacg aataaaaaat taatccagaa cacttatgga acaggttatt attatttgca
     3181 agatattgat ggtgatggaa accctgacga taaagaagag agcggagaca cgaatccata
     3241 tatcgggaaa cctgaattag aagaagtata tgatgttgac acaacagtta aggggaaagt
     3301 attcatccac gagttagcgg gaacaggtca caaagcccaa cttgttgata aagaaggtac
     3361 tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa tttcagatac
     3421 tgtagaattt gaatttacgg gtgtagattc aagtaaatta atcgcgaaag atgaattaaa
     3481 aatccaaatc gtttctccag gttttgataa accagaagaa ggttcaaccg ttattaagga
     3541 atcaccaaaa gcggttgata aacaaaccgt ggtagttgga tttaaaccag atgctaaaga
     3601 atcaattcgg aataataaaa acttacctga agatgcagag tattcatgga aaacagagcc
     3661 tgatacttct aacgttactg atagtacgaa aggtattgta actgttaaga tcggaaatcg
     3721 aactttcgac gtggatgtag agtttgctgt aaaagcttct caagctatgg aaaatgatgc
     3781 aacatacgta cctataacaa caactccaga aacgacagtt caaagtggaa aacctacatt
     3841 tgataaacca gatgttcctt tagctaaaga tgccttttca atttcagatg tctataataa
     3901 gtacttcggt aatgcaagtg ttgatgcaaa cactggtatt gttacattca ctccagctaa
     3961 aggtgtagga gaatcggagc cgattactgg aacaattcct attaaaattg tttaccaaga
     4021 tggttctgta ggcacgaccg atttagcagt aactgtaagt aaagatattt atgaaaatcc
     4081 aggagaaaac attcctgcag gctaccacaa agtaaccttc accgcagg
//
LOCUS       DARDHU010000151         4043 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000151 DARDHU010000000
VERSION     DARDHU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.155"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(153..1658)
                     /locus_tag="SDD03_002694"
     CDS             complement(153..1658)
                     /locus_tag="SDD03_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4305097.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="SDD03_002695"
     CDS             complement(1655..2341)
                     /locus_tag="SDD03_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4305098.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="SDD03_002696"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="SDD03_002696"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG4305099.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1115 a          856 c          691 g         1381 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat att
//
LOCUS       DARDHU010000152         4034 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000152 DARDHU010000000
VERSION     DARDHU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.156"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..391)
                     /locus_tag="SDD03_002697"
     CDS             complement(242..391)
                     /locus_tag="SDD03_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305100.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="SDD03_002698"
     CDS             853..1065
                     /locus_tag="SDD03_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305101.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="SDD03_002699"
     CDS             complement(1343..3142)
                     /locus_tag="SDD03_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG4305102.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="SDD03_002700"
     CDS             complement(3266..3862)
                     /locus_tag="SDD03_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4305103.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1185 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttt
//
LOCUS       DARDHU010000153         3957 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000153 DARDHU010000000
VERSION     DARDHU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.157"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1150
                     /locus_tag="SDD03_002701"
     CDS             <1..1150
                     /locus_tag="SDD03_002701"
                     /inference="COORDINATES: protein motif:HMM:NF016901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4305104.1"
                     /translation="IQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQ
                     SFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYV
                     PEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEED
                     LLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKK
                     VDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRD
                     IFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDK
                     KAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1219..2559)
                     /locus_tag="SDD03_002702"
     CDS             complement(1219..2559)
                     /locus_tag="SDD03_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4305105.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(2718..3158)
                     /locus_tag="SDD03_002703"
     CDS             complement(2718..3158)
                     /locus_tag="SDD03_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG4305106.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            3318..3824
                     /locus_tag="SDD03_002704"
     CDS             3318..3824
                     /locus_tag="SDD03_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4305107.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
BASE COUNT         1297 a          654 c          795 g         1211 t
ORIGIN      
        1 gattcaaaga aaattaagag aacttaatac tattttgaaa aactatcatt tatcttgcag
       61 cttgaaaaaa ggtaatccga tagaaggaga agaataccgt atccgatact tttttcactt
      121 gatgtattgg cagtcattcg acgaaaatca ttctgatata ttatcaagta taaaaaataa
      181 acgtcaacta ttagaaaaat ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa
      241 gaaatggatc agcctactaa gaatttcgtt gcaacgaatg aaacaaggcc actcgattac
      301 gtatgtccca gaagagatga acaaatttgt gcatccattg ataacaaaag aagagtttac
      361 gcattacata ctagagcctt tttttgaagc aaacttctgg tcgatgaatc acatcactcc
      421 aacagagcaa gcttaccttt attttatgtt tggtgtaatg aacacctatt tggaagaaga
      481 tttgttggat actccagata aagtacaaga aagttggggc tggattgaat cgtctattgt
      541 agaaaatttt gaaaaaaact tcaagatttg ttttacaact tcagaaaaaa attatctata
      601 tgtcaatttg gcaatgatcc atttgtattc aagggttttt gggacaaaaa agaaggtgga
      661 tgcttttgga aaatcagcag aagacagaga ttttcagcat atatttccag tgatgtatcc
      721 aatcatgcaa cagtttttta gtggggtagc tcgcagaatt ccagagctta atacttatta
      781 tgaaaagaac agacggttga tttttcaata ctgtatgtta gtcagggata tttttataaa
      841 acacgaacaa cctgtcatat tctatattca atcaaagtat ggtaaaacac aggaattatg
      901 ggcaaagaaa aggatattat ctatgactga acgcccaata aaatatagtg catccgctga
      961 tcagttacct gatattgtca ttagcgatta tcctatcgac aaaaaggcat atgatcaaac
     1021 gaatacacag atattttact ggaatggaca acctacaaaa aatgattggg cgcgtctgaa
     1081 agctgaaatc ataaaaatca ggaatgagaa aaatagatcc atattggaaa aattggattt
     1141 cgttaattaa gaaaagcggc tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata
     1201 atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg ttctggcttt
     1261 agtcagttgg gtagtgttct tagttttctt gaaatatttt cctcgatata aaaagattcg
     1321 ttctcggtag ttttgaaagt ttctaaagcc ataggcaatt cgttttagta ctttgatatg
     1381 gttgttcaaa cattctatag ggccgttgga atagggaact cgcaacgctc gtttgatttc
     1441 tgttcgatat ttcttgaagg tcgtgaacac tggttgatag tgctctggca actccttgta
     1501 gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgatag cggaaagaaa
     1561 aacctggtag acttcgtagc ctcgctttaa ggagtcatca taatataata gccgatcgac
     1621 tatctccgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat gctgaccatt
     1681 caattcccac gcattctttt gaagtagtct ccagtatttc tttaagtgct tcgaccgacg
     1741 atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaagg cccgaccgat
     1801 gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac actctttcac
     1861 caatgtgcga tagggtgtat agaaatctga aaccactaga cgtacttgtt cacggacttt
     1921 tctagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt ccacaatatc
     1981 aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg agacgttttt
     2041 caccgatttg aattcatcga aacacaccac ttctggtaac ggctggttca aagaaacggt
     2101 tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt gcttcatacg
     2161 tgcaatcaaa gacatagaaa taggctcact taatagctca agaatggctt gtttgactct
     2221 gcgggcaatg gagcagcgac gatcgctaac tgaatcttct gctaaaaaag tctttccaca
     2281 gtgtttacat ttgaagcgct gtttgttaat ccgcacaatg gtcttgtagt tactgacatc
     2341 attcaataag atggatactt ttttccagcc ccatttaatg atcgtttgct tattttgata
     2401 gtggcaatgg aagcattcct ctggttgata ggtcaatgtg ccatcaaaaa caaaacatgt
     2461 ttcgtcatta atgcgctctt tgcgaaaggc atcttcatga aaggtaagat ttaggtctag
     2521 aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact cctttgaatg
     2581 gttttcgtcg actttattct aaaggatttc aaggtgtctg tttgcgttta ttagaaaaaa
     2641 gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc cagaaaagca
     2701 gattgtttat gccaaaatta agacattgca aaaagattat tttctttaaa atcgacaaga
     2761 tgaccagaaa gcaagagttc gttgagctct tgctttcttt tagttttaga aaggaacatc
     2821 ctgattttat cttcgctaaa tccagtcaaa agttttgcag attggtcgta gataatcggt
     2881 tttttcaata aagaagggga tgtacgaatc tcaatatata gttcactcaa actgagttca
     2941 tctattctgt gaacaagatt tttataacta atagaatgag tagagagcat ttcggtcacg
     3001 ccgttgtttg tcaattttag taagtggata aattcatttt tggttagtgg ttgacgaatt
     3061 aagttctgtt cgataaacgg tatatcatgg acgataagcc aacgttttgc gcgtcttgaa
     3121 gacgagcagc cagaacctgt atacaattta atcatcatac attcctccaa tagctaagta
     3181 tttatgaaat aataacatga agaaatagtc gaaaagtgcc atttagaggt gtaaaataag
     3241 aaaatgttgt cacgtataat ggaactagct agataagaaa tactattaaa tcaagaaaaa
     3301 agaaagaagg cacaagtatg tcaaacacag ccgacgcaat agtagtgatc gatttacaaa
     3361 aaggggtatg ttttgattct tcgccgatac acgaattaga aacagtgaca gcattagtga
     3421 ataaaagaat caaaacatac gaaacgattg ggaaaccagt tattttcgtt caacacgaag
     3481 atgctcaatt agtgccacaa acaaaaccat gggagctatt gtcggaatta tctattcctg
     3541 agcacagtta ttttgtgaga aaaacccatg cgaattcatt ttataagact aatttgagtg
     3601 ttttattaac tgaattggac tgtcattcga tcgaattatg tggggcacaa gcagagtatt
     3661 gtatagatac gactgtgaag tttgctcatg gattaggata ccagcttatg atgtgtagag
     3721 gagcaacgac aacttatgat aatcctttta tgaaagcaaa ggaaacaatc gctttttacg
     3781 agaaaatttg ggataaacga tttctgacat ttttggagca ataagttgtt tcttcgttta
     3841 aaaaaagcag aatggatact ggtttatcca ttctgcttta ttacgtaaaa tgagaaaata
     3901 atcctgttct atttttatgg gataaaccat ttattttttg tataaaaaat gtttgcg
//
LOCUS       DARDHU010000154         3923 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000154 DARDHU010000000
VERSION     DARDHU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.158"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(573..1130)
                     /locus_tag="SDD03_002705"
     CDS             complement(573..1130)
                     /locus_tag="SDD03_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305108.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="SDD03_002706"
     CDS             complement(1309..1920)
                     /locus_tag="SDD03_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305109.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="SDD03_002707"
     CDS             complement(2033..2290)
                     /locus_tag="SDD03_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305110.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="SDD03_002708"
     CDS             complement(2887..3165)
                     /locus_tag="SDD03_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305111.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="SDD03_002709"
     CDS             complement(3199..3450)
                     /locus_tag="SDD03_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305112.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DARDHU010000155         3876 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000155 DARDHU010000000
VERSION     DARDHU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.159"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..2257
                     /locus_tag="SDD03_002710"
     CDS             158..2257
                     /locus_tag="SDD03_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG4305113.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="SDD03_002711"
     CDS             2453..3316
                     /locus_tag="SDD03_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG4305114.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="SDD03_002712"
     CDS             3421..>3876
                     /locus_tag="SDD03_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4305115.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DARDHU010000156         3777 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000156 DARDHU010000000
VERSION     DARDHU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.160"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(256..543)
                     /locus_tag="SDD03_002713"
     CDS             complement(256..543)
                     /locus_tag="SDD03_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4305116.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(563..787)
                     /locus_tag="SDD03_002714"
     CDS             complement(563..787)
                     /locus_tag="SDD03_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG4305117.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1242..1445)
                     /locus_tag="SDD03_002715"
     CDS             complement(1242..1445)
                     /locus_tag="SDD03_002715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305118.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(1427..1960)
                     /locus_tag="SDD03_002716"
     CDS             complement(1427..1960)
                     /locus_tag="SDD03_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4305119.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1981..2925)
                     /locus_tag="SDD03_002717"
     CDS             complement(1981..2925)
                     /locus_tag="SDD03_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG4305120.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3562..3648
                     /locus_tag="SDD03_002718"
     CDS             3562..3648
                     /locus_tag="SDD03_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG4305121.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
BASE COUNT         1074 a          680 c          582 g         1441 t
ORIGIN      
        1 cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt
       61 tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga gctacatttt
      121 tttcgttgtt caaagattct tatgccattt actattaata aatctataaa tatgataatt
      181 tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt cccattttca
      241 ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat atacttgaga
      301 ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa atttatgttt
      361 taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa cactttttcc
      421 ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat agtcatctat
      481 agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt tagtaaaatc
      541 cattataata acctccctac cactaccttc taggtgccca tggaccatga ttaacccaac
      601 cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc caacattttt
      661 ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt attcctgtac
      721 cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt aatactttct
      781 ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt aaataatctt
      841 agtaatttta ttatataaca ttttattata taacaatgat taacgtcata aaaagtagtt
      901 ttgaattcat aatcgtttaa ataaaattat aagatcagtt atttaaacag ctagtagaca
      961 taatgtgtat actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat
     1021 ttttattggt ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc
     1081 taattctttg tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt
     1141 gttatgcttt ttacatatct catattcatt tattattctt ttctaaggtt aataatagga
     1201 taatctcttt cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct
     1261 acaaatactt cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt
     1321 ttatttcctt tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg
     1381 atagaaccga atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct
     1441 gccaccattt tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag
     1501 tcgttgaact attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt
     1561 tttcgatttg ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta
     1621 tatgagaatt attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac
     1681 gcaactgatc tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat
     1741 cgttgttttg ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag
     1801 caagttgcaa tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca
     1861 tggaagaatc taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat
     1921 ctgacaattc ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg
     1981 ctactggaat aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc
     2041 ggactctgtt tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag
     2101 gagttcaagt tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc
     2161 gtattctttt tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc
     2221 atgtttgtta aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat
     2281 aacaggtttt ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc
     2341 agttagttct tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact
     2401 tttaggaata tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt
     2461 tctgtattgc tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa
     2521 ttgttgtaaa gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc
     2581 gtaaattcgt atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa
     2641 cattacaaac atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa
     2701 gattttttca taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta
     2761 gttacttaaa tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg
     2821 cattctagac acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg
     2881 aattgtattt aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc
     2941 caattatgaa tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa
     3001 aatgtccttc ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac
     3061 agacaaaatg tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg
     3121 taaagaggtt taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg
     3181 cctaaaaacc acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc
     3241 aaaaccttgc ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg
     3301 cgcagtcgcc taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt
     3361 ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta
     3421 aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa
     3481 acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc
     3541 gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt
     3601 tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg
     3661 cgacatcaac ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct
     3721 aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc aattttg
//
LOCUS       DARDHU010000157         3755 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000157 DARDHU010000000
VERSION     DARDHU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.161"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            415..621
                     /locus_tag="SDD03_002719"
     CDS             415..621
                     /locus_tag="SDD03_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG4305122.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(823..1824)
                     /locus_tag="SDD03_002720"
     CDS             complement(823..1824)
                     /locus_tag="SDD03_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4305123.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1829..3742)
                     /locus_tag="SDD03_002721"
     CDS             complement(1829..3742)
                     /locus_tag="SDD03_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG4305124.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
BASE COUNT          989 a          762 c          583 g         1421 t
ORIGIN      
        1 attatttttg tttcttattt tctaaaaaat taaaaaaaat tttggtgaat taagtatttc
       61 cttgataaca ctggcttttg gcctcatttt aggttatcat ttgtaatttt atttcacagt
      121 tgatatgatt gttatgtaaa ataaaaaagt ttaggataca tcgaattttt ggggaaattc
      181 ttttgggggt atcctaaatc ttttctatta tattcccata ttttctatta ttggtctaga
      241 cgtatgatgg taaactttgt acctatctga ttgctgaatc attaattgct tatcattagc
      301 catctttgtt tcaagttcgc tagatgatca ttttattcac atcctaacaa acttgtcaca
      361 tttatagatt cttaatctat ttatcataca attataaaaa aggaggtgct gcttatgaaa
      421 gctatccata tttctttgct ttttttcgct agtgcattga ttttatttgc tattcatctt
      481 tgttcccagc cttatatcaa taacaacgga atattagtgg aaccagcatt cttttgctta
      541 ccactcggct ttttgtccct tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa
      601 cgatcaaaac aacaaatctg acttataaaa tttacgtaaa agagatcgtg acaataatcg
      661 tgtgatattc ccaaatttcg aataaaaggt gccccttgcc atctctaact cctcagtcac
      721 aagttacaat attcataaac catgaaaagc ttgtttattg aaattgctcc ttgtgacttg
      781 gagctaagtg acagattaca acctcttttg catcataaga ctttactcct tgaaaaacat
      841 tactaacttt tgatatgttt cttgcgcttg tttattgctg aaatcaaact gatattcatg
      901 gttcaaaaca gcatttttct ccgcataatc tacttttgtg actttcaccc ccgctttctc
      961 aaatacagaa gttacttctt ccatttgttt cgtaaacgta tttgtatttc catccgttaa
     1021 gaatgtgttt tgcgtccaac gatccgcatg tctgaccaag gctaaacttt tgaccttctc
     1081 tgattgttca aagtccttat tatcaaagta agcccatcca catttttcat aaagaaagct
     1141 agagatttta tcacttactt gagtaagtgc agaaggatcc aacagaccag acaaagaaat
     1201 aaacttcgta atctgcttgt tctccaaagt cggatcaaga tgatcagctt ttttaatttc
     1261 ttcattttga agtgctaaca aatattctcc actaatttgt gcacctgccg aatttccacc
     1321 aatcgaaata ttcgaccaat ctgcttgatc ttttatctct tttttagctg cttgaatggc
     1381 ttcgttcaat tggataactg gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat
     1441 atttacgatg atttgctgcg tgtctgaaca tagatattcc aaataatcac gacaatcatt
     1501 tttatcccct cctacatatg cgcctccatg catccagaaa agaatcggta attttttctt
     1561 ttccactttc ggtgtgtaga tatccatatt attatttttg tactttgaag gataggatat
     1621 ttcttttttt atatcgattt gttcgatttt ttgctggaaa tcatctggtc gggcatatac
     1681 ttgttctttt tcacttgtgt caaattgttt tctaacaagc aagctaatcg gctttggtgt
     1741 gatttgatta atcaaaaata cagtgactaa aataactacg atgataactg gtactgcaat
     1801 cactattcgc ttgaaccatt tcattcattt accctctctt tcttcgccaa atccatgtga
     1861 taatcgtgct aacagataaa aatccacctg aataaatgag aagcgaatgt ttcttttctc
     1921 cagttttcgg caacattctt ttttttgcat ctgtcttttt ttgtactttc actgtcatct
     1981 gaatcggttg cgtatcatca cttgattgat cctctcgagg ttcattcttt cccttattgt
     2041 ctgcattctc tgtcggatct tgcttttttg gtggagctac tgggattttt ttgtaataga
     2101 aaataatagt tggtatcgtc tcattaaaaa tcccttcagt cggtccttca acatttgcta
     2161 attcgtatcc ttcgaatgtc ttttgctctg tcatataatt ttctccataa gctccgtaaa
     2221 gctggatttc gtcctggtct ttgagtagac ttttattgtt ttcatccaca tactttacgt
     2281 ttatctgatc atggaacact actttcaaaa agtccgttac atgtttcgtg taggcagcat
     2341 ggtcaacttc ctgtgccttt ccatgccctg ctccttctac taataaaaga tctttatacc
     2401 ctcctgataa ctcatataat ttttgcgcgt tagacacagg tactacatca tctgctgttc
     2461 catggataat caatttaggc aatgaatctg acattccaat cgtaggtaga gcagcttttg
     2521 tatccaaatg catctcctga tcaaaatagt attgtttcaa aaggcccata aataaatctt
     2581 catgctcttg atccccaatg atgtctaact gattttttat ggcatccggt agtgcggggg
     2641 ctactgtctg gctgtacgta aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt
     2701 caataattcc tttgacattg acaggaatat ctttcgctaa tacagacatc atcgtagctg
     2761 ctcccatcga accaccataa agtaaaatct tttgagatgg ccgctctcga acttcttgat
     2821 ttatccagta aaggacgtca tctgactcat attgtccata ggtcacataa tcgcctccgc
     2881 tattaccagt cgctctggaa tcaacaaata ataaattata cccggcctta taaaaatcat
     2941 tgtattcttc tgtaacgatc ccgttattcc aattccctcg aaatccgcca tggatcatga
     3001 ctgttttatc tgaaccttga tcgacataat atgcatatac atccatcatt ttgccatctt
     3061 gtttgtttag tacagtttga tgaagttgtt ttgtatttgg atagaagcct gtgttttctg
     3121 taggcgttgt cccatcatct gtacctccaa gcatcgacat caacacactg ttttttgcaa
     3181 aagcgatatc gtacaataga taagtagcaa tcagatgttt gataatctct ttatctacac
     3241 cataatcatt aacagcaagc tcagtgacta ataactctat taattttgac ttgatgtctt
     3301 ctgttggtcc attaagtatt tctgtcatct gtttaccgaa gtaattcaaa tattctttac
     3361 cattgaaatt ttcaccttta ccgatatcat cattttctgt cccattctct ttgtcactta
     3421 ctttagcaga agaggattcg tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt
     3481 ggttatctgt ggtagcggtc gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg
     3541 tttcagtatt tgtttctgtt tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag
     3601 tttctgtggt ttctacctcc cgagattccg attcggaaga aatgctctct ccatatactg
     3661 tccattgcgg gacgcttgta cttacagcaa ataagatgac caacgctccc tttaatactt
     3721 gttttgttct caagctattc actccttttt atgtt
//
LOCUS       DARDHU010000158         3717 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000158 DARDHU010000000
VERSION     DARDHU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.162"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..2542
                     /locus_tag="SDD03_002722"
     CDS             101..2542
                     /locus_tag="SDD03_002722"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG4305125.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="SDD03_002723"
     CDS             complement(2872..3576)
                     /locus_tag="SDD03_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305126.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1193 a          654 c          813 g         1057 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaa
//
LOCUS       DARDHU010000159         3712 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000159 DARDHU010000000
VERSION     DARDHU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.163"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SDD03_002724"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SDD03_002724"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG4305127.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="SDD03_002725"
     CDS             complement(2260..2511)
                     /locus_tag="SDD03_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG4305128.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="SDD03_002726"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="SDD03_002726"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG4305129.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="SDD03_002727"
     CDS             3461..3679
                     /locus_tag="SDD03_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305130.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DARDHU010000160         3681 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000160 DARDHU010000000
VERSION     DARDHU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.164"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(141..314)
                     /locus_tag="SDD03_002728"
     CDS             complement(141..314)
                     /locus_tag="SDD03_002728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305131.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            600..1928
                     /locus_tag="SDD03_002729"
     CDS             600..1928
                     /locus_tag="SDD03_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG4305132.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2130..2963
                     /locus_tag="SDD03_002730"
     CDS             2130..2963
                     /locus_tag="SDD03_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG4305133.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2979..>3681
                     /locus_tag="SDD03_002731"
     CDS             2979..>3681
                     /locus_tag="SDD03_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG4305134.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQL"
BASE COUNT         1224 a          582 c          766 g         1109 t
ORIGIN      
        1 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
       61 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
      121 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
      181 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
      241 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
      301 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
      361 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
      421 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
      481 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
      541 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
      601 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
      661 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
      721 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
      781 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
      841 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
      901 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
      961 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     1021 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     1081 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     1141 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     1201 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     1261 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     1321 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     1381 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     1441 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     1501 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     1561 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     1621 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     1681 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     1741 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     1801 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     1861 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     1921 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     1981 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     2041 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     2101 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     2161 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     2221 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     2281 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     2341 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     2401 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     2461 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     2521 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     2581 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     2641 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     2701 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     2761 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     2821 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     2881 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     2941 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     3001 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     3061 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     3121 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     3181 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     3241 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     3301 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     3361 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     3421 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     3481 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     3541 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     3601 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     3661 tccgaatgca agaacagctg t
//
LOCUS       DARDHU010000161         3659 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000161 DARDHU010000000
VERSION     DARDHU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.165"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1166
                     /locus_tag="SDD03_002732"
     CDS             <1..1166
                     /locus_tag="SDD03_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305135.1"
                     /translation="TSLSMRYFIMAIWDLYFMLSYFSYREKTYKKYVIKNNFWLTIMP
                     FVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLILAYGYACTAFYLFKAEGIDFIFWS
                     GVISYMSGSVFYLIVSYGLNGLISYIKSLITGETNTANFAMEVHDLTFAMGVFFLYYV
                     FFENKNTKKHKIKIIVSLILIFMGLKRIELLALSGAILVYYVLLKWGKKIQIRALVCT
                     ICATVISLVFIYIIDTGILALLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTW
                     FSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTKK
                     FGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMIFLLVPMIVSWVDNQQRISE"
     gene            1250..2338
                     /locus_tag="SDD03_002733"
     CDS             1250..2338
                     /locus_tag="SDD03_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305136.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            2335..>3659
                     /locus_tag="SDD03_002734"
     CDS             2335..>3659
                     /locus_tag="SDD03_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG4305137.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIM"
BASE COUNT         1275 a          401 c          641 g         1342 t
ORIGIN      
        1 atactagttt atctatgcga tattttatta tggcaatatg ggatttatat tttatgttaa
       61 gctatttttc ttatcgtgag aaaacatata aaaaatacgt tatcaagaat aacttttggt
      121 tgactataat gccgtttgtg atattttcta tctatactct aataatatgg ggaattggtt
      181 caaatgcaga atttgaaaat tttataaagt tatgttccac gttgctttat ttaattcttg
      241 catatggtta cgcatgtaca gcattttact tatttaaggc agaaggaatt gattttattt
      301 tttggtcagg tgttatttca tatatgagtg gttcagtttt ttatttaatt gtatcttatg
      361 gtttaaatgg tttaatttct tatataaaaa gtcttataac aggtgaaaca aatactgcaa
      421 attttgctat ggaggtacat gatttaactt ttgcgatggg ggtttttttc ctttattacg
      481 tattttttga aaataaaaat acaaaaaaac ataagataaa aattatcgta tctttaattt
      541 taattttcat gggattgaag agaattgaac tgttagcttt aagtggtgca attctagtgt
      601 attatgtttt attaaagtgg ggaaaaaaga ttcaaatccg tgcattagtg tgtactattt
      661 gtgcaactgt tatttcgtta gtgtttattt acattataga tacaggaatt ttggcattgc
      721 ttatgtcaaa tttgggcatt acagatgacg gtgcgaggct ttcattttat ctatattcat
      781 ctaaatttta tgaacttggt ttaggatatg taggaacagg atggacatgg ttttcaaaat
      841 acttttttaa tctttattta tcgcacttta ggttaaatgg tggaagaatt gcagcaagcc
      901 tacacagtga tatattaact atttatatag aagttggatg tatagttttt attatatgga
      961 tgttttggtt attttttgtg aaaccgataa gactaactaa aaaatttgga ttgaaaacag
     1021 gggagtgtgt attattatta actatatata tgtttatatt atatttgacg gataatacgc
     1081 tcacttatcc agacactcaa atgatttttc tcttagtacc tatgatagtt tcgtgggttg
     1141 ataatcaaca acgaatatcg gaatagatag ctaaagtata ttttaattat gaatttataa
     1201 ttagttgtaa aagttaatat ttaacggaca ggaagtaggg tgaaaaaatt tgaaaaaaat
     1261 tttagtgtta gcaaatacat attatcaaat aatagtagca atacaaatga aaaatacaat
     1321 tttttccgat gagtatgttg aaattattat ttctgatcat tctaaaaaca ctaaagaaat
     1381 tgcgaaaaaa atagaagaaa aaaaagtgtt tgatggctgt cattatttag aaagcaaatc
     1441 ttttgatgat aatattgggt attttcaaaa gattaaggac tgtcttcaga tttcttgtgg
     1501 taaatcaaat cgatatgcct attatttgaa aaactgtaaa agtgaatatt ttgatgaatt
     1561 tatttgtttt tcaatgggag aggtttggtt ggaaggaata tatgcttgct tatatcgaaa
     1621 taataaaaat gtacaggtat caattttaga agaaggtatt ttaagctatg gagttgacat
     1681 tattccaaca gcaagaacaa aagctatcgc agtactcaat tacttaaaaa aaaggactcc
     1741 actatataac aaaagctata atttttattg tttttttcct aatatatata caggaagaga
     1801 gaatacaata caaattccat tagttacagc tgggtcaaga accgcattgc aactttcgga
     1861 tatttttaac cctatgatag atatttataa agaaaaaaaa tatatatttt ttacgtctgt
     1921 ttatgatttt gagggcggag aacctgtcgg agaatatgaa ttagtatgca aaatagcaga
     1981 cttagtagga aaagataatc taattgttaa gacgcatcct agagatgtaa gaacaattta
     2041 taaagataat ggatttatcg aggataaaaa ttcatcaata ccttgggagg cgattcaatt
     2101 gtcaggcgat tttaacgata aaatatttat gacaattaat tcaggaagtg ttatttcagg
     2161 tagtacaatg tctgcaaaac ctgtacgcac gtattatatg tataaattgt gtaacataga
     2221 aggaaatccg tcttgcaaaa agaatgcgta tgatattgag cgattgctta agcaaaaaac
     2281 gatgaaaaac attttaaaga atgtgaaaat agcagaaagc ttagatgata tactatgagc
     2341 aaaactaaat atttaattaa aaatatggga atattaacaa taagtaattt ttcttcaaaa
     2401 attttagttt ttttgttagt ccctttatat acaagtgtat tatccacaac agaatatggc
     2461 acatatgatt tgacaatttc aacagccaca ttattgtttc caattttaac cttaaacatt
     2521 gtagatgctg tgatgcgctt tttaatggat aaagataaaa ataaaaaaaa tatagcatct
     2581 atagcgatta aagttatatt tttaagcgtg atagtctttg gatccgtaat gtatttgtta
     2641 aatattacaa aattatggca agatgtttat ggattagaaa aatatatttt tttgtattat
     2701 atatcctatg tatggaatca atttctaata caatttgcta aagggttaga aaaagttaaa
     2761 gctatgggca tagcaggtat tatcagcaca attgtaacaa taattgccaa tattttcttc
     2821 ttgcttattc tgaaatgggg attaattggt tttttcattg caaatatttt atcacagatt
     2881 atttcagcta cttatctgtc agtattagtg agaatatggg aatatatcga ttttgaggct
     2941 aaagatcctc agctaaagag agaaatgcta aattactcga ttccattaat tgcaactgtt
     3001 gttggatggt ggattaatag tacagctgat aggtatgtgg tttcctttat agtaggtgtc
     3061 tcggcgaatg gcttattgtc tgtggcatat aaaatacctc aaataattaa tactttgcag
     3121 ggaatattta tacaggcctg gcaaatttct gcaattaaag aatatggaga taatgagact
     3181 gcaaaatttt atggaaatac atttgttatg attaatttgt taatgtgcat aacctgttca
     3241 agtcttatca tattaactag accgttttca catattcttt atgcgaaaga cttttatgaa
     3301 gcgtggaaat atgttccatt tttattaata gctaatgttt taaattgtgc atcaggtctg
     3361 ttaggaccta tcttatcagc aaaaaaagat tctaaatcca tgatgtggtc tgcaatcata
     3421 ggggcattag ttaatgtgat tatgagtgta gtccttgttt ataagatagg ggttcaaggt
     3481 gccacaatag ccactgttat atgttcatat gtaatatatg ttgttcgtaa acgagctgtg
     3541 ggaaaagata tttttataaa gcattatcat attgttgtgg aatcttgggg attattgttt
     3601 gttcaggcaa tcatagaaat atattttaaa aattatgtaa tagaaattat aattatgat
//
LOCUS       DARDHU010000162         3601 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000162 DARDHU010000000
VERSION     DARDHU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.166"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SDD03_002735"
     CDS             complement(<1..1256)
                     /locus_tag="SDD03_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4305138.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SDD03_002736"
     CDS             complement(1270..2487)
                     /locus_tag="SDD03_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4305139.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>3601)
                     /locus_tag="SDD03_002737"
     CDS             complement(2477..>3601)
                     /locus_tag="SDD03_002737"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG4305140.1"
                     /translation="IKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          991 a          784 c          605 g         1221 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 t
//
LOCUS       DARDHU010000163         3600 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000163 DARDHU010000000
VERSION     DARDHU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.167"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1125
                     /locus_tag="SDD03_002738"
     CDS             <1..1125
                     /locus_tag="SDD03_002738"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG4305141.1"
                     /translation="IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1115..2332
                     /locus_tag="SDD03_002739"
     CDS             1115..2332
                     /locus_tag="SDD03_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4305142.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2345..>3600
                     /locus_tag="SDD03_002740"
     CDS             2345..>3600
                     /locus_tag="SDD03_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4305143.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1239 a          614 c          756 g          991 t
ORIGIN      
        1 atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg
       61 tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac
      121 acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga
      181 cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt
      241 aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca
      301 tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc
      361 cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca
      421 gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat
      481 tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt
      541 cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga
      601 gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt
      661 atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg
      721 gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt
      781 gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca aaaaatcctc
      841 gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc atttggcaac ctttgatgaa
      901 atcaaagaaa atgactataa cttaaatatc ccacgctatg tagatacttt cgaagaagaa
      961 gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca agaacagcaa
     1021 gtgctagaaa aaaaactttt agaagctatc tcttccctgc aaacaacacc tgaaaatgaa
     1081 gcgtggttac aaggtgcgct ggaggtgttc aaacatgagc aatgatacac aaccagaaat
     1141 ccgtttccca ggatttacag atgattggga acagaggaag ttgggggact tcgttgtaga
     1201 ttatgttgaa aagacaagtg tacaaaatca atttcctatg ctaacttcat ctcaacaaaa
     1261 agggatagtt ttgcaagaag actattttgc aaatcggcaa gtaactacag aaaataatat
     1321 tggttacttt gtactaccta gaggatattt cacgttccgt agtcgaagtg ataatgatgt
     1381 gtttgtgttt aaccgtaatg acattattga tagagggatt ataagctatt tttatcctgt
     1441 atttacttta aaatctgctg attccgactt cttcttaaga cgtattaaca atggaatcca
     1501 acgacaatta tctattcaag cagagggaac aggtcaacat gttctgtctc taaaaaagtt
     1561 taaaaatata gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg gcaccttctt
     1621 caaacaactt gacgacacga tcgctcttca tcagcgtaag ttagatttgc tgaaggaaac
     1681 gaaaaaagga ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc cagaaatccg
     1741 gtttcctggt tttacggaag attgggaaga gcgtaagttg ggagaattag gtaaaactca
     1801 atcgggaatt ggatttcctg atgccgagca aggtggttct gaaggtactc cattttttaa
     1861 agtttccgac atgaataaca ttggtaatga gtatgaaatg agaaatgcaa atcattatgt
     1921 atctaatgag caaattgagc gtaaaaaatg gaagccaata aaagatgtgc cagcgattat
     1981 atttgctaaa gttggcgcag caataatgct taatagaaaa cgtttggtca caagcccttt
     2041 cttaatagat aacaatacca tggcttattt atttaataat acttgggata tctattttgg
     2101 taaaatattg tttgaaacta taaatttgcc aagatactcc caagtagggg cattgccttc
     2161 ttataacagt tctgatattg agaatatcag tgttaaggtt cctgtaaagg atgaacaaca
     2221 aaaaatcggt actttcttca aacagctaga tgatacgatc gctcttcatc aacgtaagtt
     2281 agatttacta aaagaaacga aaaaaagctt cttacaaaag atgtttgtct aggagggaag
     2341 aagaatgaaa attagtcacc gtgaggaagc agaagtcgaa gaacagttga ttcgtgtact
     2401 tggagaaggg cataatcagt ggacgtatcg tccggatcta aaatcagaag aagacctttg
     2461 ggtcaattta cgccaaaaaa ttatctcaaa taatcaagca gaactgaatg attttccttt
     2521 gaccgataaa gaatttgaaa cgatcaaaac ggaattactg ctgcgaacga aaaccccatt
     2581 tgatgctgcg aaatggttaa aaggtgaaaa tgggatggct cggatcacga ttgaacggga
     2641 agattcgcaa ttgggttctg tctcacttat cctttattcc aatcaagaca ttggtggagg
     2701 aatctctact tatgaagtgg tccatcaaat tgcaaaacgt ggaagtaaca tagaagcacg
     2761 ggatcgacgt tttgacgtca ccttattaat caatggatta ccgattgtcc aaatcgagct
     2821 gaaacaagta actgcaaaag atggtgttta tcaggcattt aaccaaatca aaaaatatgc
     2881 agaagaaggt atgtttcgga acaatatctt ttcgacctta cagttgtttg tggtttccaa
     2941 tgaacaaacc actcggtatt ttgcaaatgc cttaccgaaa gacttgcacc ctaagttttt
     3001 atttagttgg cgaacgaaag acaatgaaaa agtagaaaac ttatatgagt tttgtaaaca
     3061 agtgttaaat atcccggatg ctcatcgttt gattgcggat tatacaatcg tcagtgaaga
     3121 tcaagacaat aaaaccctca tggtgttaca tccttatcaa gtacatgcga ttcaagcctt
     3181 gtttattgcc gcaaataaac atcaatcagg gtatgtgtgg catgcgactg gttcaggaaa
     3241 gacgttgact agttttgtat ctactaagct tttagcacga aaatcgggga tcgatcgaac
     3301 gatcatgtta gtggaccgca aagatttaga taatcaaacc accacagagt ttacaaaatt
     3361 tgcttcggaa tttaatactg ggatttcttc cgggaacgcc aaagcgaata gcttgatcgt
     3421 tggaacggga agtgccaaag aattaagtga aaccctgttg gcagatgcca atgcaaatgt
     3481 tgtaatcatt acaacccgtc aaaaactaga tgcggcgttg aaatatgcaa agaaacaaga
     3541 agagaaaaaa ggaaccaatc gcttccaaaa attaatgggc caacatatcg tgtttgtagt
//
LOCUS       DARDHU010000164         3565 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000164 DARDHU010000000
VERSION     DARDHU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.168"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            155..2344
                     /locus_tag="SDD03_002741"
     CDS             155..2344
                     /locus_tag="SDD03_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4305144.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..>3565
                     /locus_tag="SDD03_002742"
     CDS             2341..>3565
                     /locus_tag="SDD03_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG4305145.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDI"
BASE COUNT         1349 a          481 c          593 g         1142 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     2701 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     2761 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     2821 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     2881 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2941 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     3001 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     3061 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     3121 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     3181 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     3241 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     3301 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     3361 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     3421 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     3481 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     3541 agaaagctaa ttgcaaacga tattt
//
LOCUS       DARDHU010000165         3287 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000165 DARDHU010000000
VERSION     DARDHU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.169"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..817
                     /locus_tag="SDD03_002743"
     CDS             101..817
                     /locus_tag="SDD03_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG4305146.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            836..2419
                     /locus_tag="SDD03_002744"
     CDS             836..2419
                     /locus_tag="SDD03_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG4305147.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2554..3132
                     /locus_tag="SDD03_002745"
     CDS             2554..3132
                     /locus_tag="SDD03_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305148.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
BASE COUNT         1241 a          363 c          614 g         1069 t
ORIGIN      
        1 agcagttgtg ttatagagga agaaacatta tttcaatgat taagagtcga gagcacaaac
       61 ggtagacgat gttattttta taaaaagata taatttatta atggtcgata attatgaaag
      121 gaaaaatatt cttaaaaaaa agagtatagg atgtcgtatg aaaatagtcg ctatagtacc
      181 aatgaagtta aataacaaaa gattgccaca aaaaaacacg aaagctttta ccaatggaaa
      241 accattgtgt tattatatac tttcaacact tttaaaagta aaacagattg ataaagtgta
      301 tgtctattgt agtaacccag atattaaaga atttatacca gaaggtgtaa catttttaaa
      361 gagatctagt tccttagatc aggatacaac gagtattaca caggtattag ccggtttcac
      421 aaaagaagta gaggctgatg tatatgtaat gacacacaca acagcaccat ttattagcga
      481 aaaatctatt gaaaaaggac tagatgccgt gctgacagga gaatatgatt ctgcatttgc
      541 tgcaaaaaaa ttgcaagatt tcttatggaa agacggtgaa ccatttaatt atgaattgga
      601 caatattcct agaacacagg atttgccgcc tttatacgaa gaaactagtg gattttatat
      661 ttataatcgc aatgtgatga ccgagttgca tagaagaatt gggaaaaaac cttgcatagt
      721 tgaagtaggt gagattgagt cagttgatat tgatgaagcc gaggatttta tgattgctga
      781 tgcgatttat aatcatctta ttttacaagg tagttaagac agggggaagg ttaggatgaa
      841 caatgtacag attttagatt gcacacttag agatgggggc tattgtaaca attgggaatt
      901 tggatttgat aatacaaaaa aaattatttc tggacttata gaatcaggaa ttgagattat
      961 agaatgtggg tttctcacaa ataaaacagt atacaataaa aatgttacaa aatatacgac
     1021 cttagaagaa ttagcacaga taattcctga aaaaagagaa ggaaaattat ttgtagcaat
     1081 gatgaactat ggcgaatata atatagagga tcttccagat tatgatggtt catctattga
     1141 tggaattagg attgcgtttc ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga
     1201 gataaagaaa aaaggctact tagtttttgt tcaagctatg gtatctctca attatacaga
     1261 tgaagagttt ttagcagtaa taaaaaatgt gaatcagttt aaaccatacg ctttttatat
     1321 tgttgatagt tttggaatga tgaaaggaaa agatttaact cgtctttttt acatgattga
     1381 gcataatcta gaaaaagata tacagatagg ttttcactca cataataaca tgcagcttgc
     1441 atattctaat gcacaaaaac ttataaccgt acaaacaaat cgaagcctga ttattgattc
     1501 ttcggtgtac ggaatgggaa gaggagcagg taatcttaac acagaactat ttgtagaata
     1561 tttgaatgat aatgcaggaa aaaaatatca attaaaacca ttattatata taattgatga
     1621 aatactaaat agtttctatc agaagagtta ttggggatat tcattaccaa actatatttc
     1681 agcagttcat aatgctcatc ctaattatgc aagctatctg tcatataaga atactttaac
     1741 aattgaagca atgaatgaaa tattcgaaat gatggatgaa gaaaaaaagg tatcttatga
     1801 taaacaatat atagaagatt tatactacaa atatatggaa actgggaaaa tacaggagga
     1861 atatatggaa gaactgagaa aatttctctc agacaaaaaa attctactga ttgcaccagg
     1921 aaaaagttct gtagatgagg tagaaagaat aaaagagttt gttaccaaag aagaagtcgt
     1981 aataattagt gtaaattttg aatattctca tatagatata gattacgtat ttttaagtaa
     2041 tttaagacgt tttcatgaat taccgaaaga aagcagaact aaatgtattg ttacatcaaa
     2101 tatacaagca tcggatgtat atttacggac gagttataaa aagttgttat ctgaaaggga
     2161 agttgtaaga gacaatgcag gattaatggc aattagattt ttagccgata tgaatgtgga
     2221 tgaaatattc ttagctggat ttgatggata ttcacataat gaagatgaga attatggtga
     2281 gcaatccatg gaaattataa caaaatttgc attattagat gcaatgaacg tgcaaatgag
     2341 agatgaattg tcagaattag ctaaaaaagt aaaggtgaca tttttaacgg atccacgttg
     2401 ggttcgaatt gataagtagt agtaataatg tttttaaaat tttgataaag agatagtgag
     2461 agaataaaaa tgggtaaagt cttaaataaa aaagaagaaa aatatttaag ggacgatggg
     2521 gcgagatgaa actctaattt tgaactttta agaatcattt ctatattgtg tattgttgct
     2581 taccatgcta atttgcgttt aaattataat gcagacggaa ttatagtaca tggttcatta
     2641 actgctaacc agattttctt gaattatatt ggtatttggg gtcagctagg agttaattgt
     2701 tttatattta tttcggcata ttttttaagt gagaaaagaa acattcactt tgaaaatgtt
     2761 ttgggaattg tattagaatg tattttttat agtgtattag tttatggaat tctttgtgca
     2821 tgtggaatag aaacattttc attgaacagc tttgtaaaag aaatattatc atttgctcta
     2881 ggtagatatt ggtttattac atcctattta gcattgtata ttctaagtcc gtatttaaat
     2941 atcatgctta acaatctgga aaaacttcag ttgcaaaaaa taagtatgtt attgtttata
     3001 ggtgtatgct gttatcgtat gttatttaaa acatctttgt ttggggattg tgctgtttta
     3061 attgcactat atgtcatatg ttatacactg aaaaaaacga taataatatt tttgagagac
     3121 acagaatcat aggaacaatt gtatgtataa cagttatacc acttctcata tccataggaa
     3181 tacttttcaa atttatacca aatgcaatgg ttaaccagat ggttcatatt ggttctccat
     3241 tgatggttat agcaagtata tttttatttt atgtatttaa aaatttt
//
LOCUS       DARDHU010000166         3220 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000166 DARDHU010000000
VERSION     DARDHU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.170"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..170
                     /locus_tag="SDD03_002746"
     CDS             <1..170
                     /locus_tag="SDD03_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305149.1"
                     /translation="TGVLIGTNSKVYQEVKEKIEAKLNPSNRNRPKNHQKETSQTVTS
                     RNFDPTNFLKR"
     gene            complement(307..1026)
                     /locus_tag="SDD03_002747"
     CDS             complement(307..1026)
                     /locus_tag="SDD03_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4305150.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            1701..1796
                     /locus_tag="SDD03_002748"
     CDS             1701..1796
                     /locus_tag="SDD03_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG4305151.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2098..2343)
                     /locus_tag="SDD03_002749"
     CDS             complement(2098..2343)
                     /locus_tag="SDD03_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305152.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            2688..2972
                     /locus_tag="SDD03_002750"
     CDS             2688..2972
                     /locus_tag="SDD03_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305153.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2975..>3220
                     /locus_tag="SDD03_002751"
     CDS             2975..>3220
                     /locus_tag="SDD03_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305154.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLK"
BASE COUNT         1074 a          599 c          502 g         1045 t
ORIGIN      
        1 atacaggcgt tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg
       61 aagctaaact aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc
      121 aaacagtcac ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata
      181 ggagttattc ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag
      241 caatcttagt cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt
      301 ttttatttat tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct
      361 tgaaaaagta aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac
      421 tttaagatca ttgaagtatt tttttagttc tcgtaatgca ggatttaata ctttttgatc
      481 aatgttaccc atttgatagt attctgggat atccattaag ctcctaaact cttcaatacg
      541 aactacataa taaccagtag aacggtactg cataagcagt cggaatagag tttttgcata
      601 cgtagaacgc aacgacgtga aagcagataa ttcaaatcta ctaaattcag ctgttaaacc
      661 attaataata tattctaggt ccggattaac actgacttca acaaactttt ttgttttatt
      721 gatttcaaaa ccagtaaaca aaacaaagta gcgaatagaa tcctcatctt cagtacggta
      781 agtaagatta agcatatctt tatagagctt ttctaaatca ccgacaaagg cttttgtagc
      841 agtcatctta taatcactta actcttttaa ttcctcgaaa gaaaatctaa cttttttcaa
      901 accacgatct ttcatacgag cgcataatgc aaaaaataaa tccatctgct tagcattgaa
      961 atttttcagc ggtactaaat tcaattcatt ttgatatttg actgttaatt ccgttttttc
     1021 atccattcct ttcttccccc cttacaaata aataataacc tttaacaacc aactcgtaaa
     1081 taggttggtt gttattatac aaaaggatat tagtactcag ataaaaagtt gttagtactc
     1141 agataaaaag ttgttagtac tcagataaaa agttgttagt actcagataa aaagttgtta
     1201 gtactcagat aaaaagttgt tagtaccctt ctgtatccgt tgggagagta aggctcaact
     1261 gccccctaaa aagggttcta aaaaatcttt aaaaaataaa taaaataaac gcgcgcgcgc
     1321 gagcaaaatt tcccgccaaa aaatcaccca ctttttaaaa taaacaggtt cgcgcatgat
     1381 attaaactgc caatctagct ccctatggtc gctgtaagaa ggagtgatcg ctacgctctc
     1441 acattttact tgcagcagtc gcctttaggc tccttcgcca aaaataattc attagggact
     1501 cctttgtggc gtcctgagag ctcgtctaag ccgttctaag acaagattcg ataaaaacta
     1561 tgtccttcgt tctaaagcgc cttacaggcg gtcagggcaa gccctaacaa ccccaaggca
     1621 ataaaaaact taggcttatt tttgcttttt tattgtataa ttgattgtcg cctatccgac
     1681 agtcttgcga ctggaaggag gtgtgtttcg tgttcactaa ctttatttgc tcccttcttg
     1741 ttgggctttt aagcgctttg ttcagttact ggttgaataa ccgaaacaag aagtaaaact
     1801 taattaaggc gactcagccc acaaaaaaag ccccagagaa tccgacctct ctggggcttt
     1861 cgtgtgtttc gtgttcactt atttgcaatc aaattataac agaaataaat atttatttca
     1921 aaaatatcct acaaaagtct aaaatccgta attatttctt atatgcaaca tttacaaata
     1981 aagtcgctct gagagctcgt atttttcgat ttaagacgtt ttatttagtt tttggtgtat
     2041 ttcattggtc caagctaaaa aactcttaga agcgatttta gagccattta aacccactta
     2101 attatattct ccatgaaatt cgctccatgc gtcaaaagct gcgtctgaaa ttccgccgtc
     2161 ttcgttcact agttgcatta atttttccaa cgatcgtttt ctttcgggtg tcatattttt
     2221 atattccaac aatttttcgt ctgttaaatc gaacttgatt gcttgaattg cttgctctaa
     2281 tttttctaag ttttgaaaat attcgtaagg aaatttttcc attttttctt tctccagtct
     2341 cacaattttt ctagaaataa aatcacttct ttgccgtcta tactctcaaa aaaaatgtcg
     2401 tttgaaaaac taaataaatt tttgatcgtg catttttaaa tttggttatc gattacacgt
     2461 gcagcactcc cccccgaatt ttaaagagca taaggaaacg tgtacccact ttctaaaaaa
     2521 agtgggtaga taggctgtgc ctatcacgtt tctcagaaaa aaatttgata tcactttttc
     2581 gccatcgtgg gtagcttatg tatagcattt tggcaaaaaa cagctataca aatatagtat
     2641 ttgtatggta tacataaatt atacataatt gcaaaaggag attttttatg gacattttag
     2701 ttcggaatat tgatccaaag tttgttgcaa caatagacaa acgttgccaa gaactcacga
     2761 ataaaacagg agaaaaatgg agtcgaaata aatatctaaa agttttaatc gaaagtgatt
     2821 ttgaccgtca acttacgact tacaaaaaag accaattcga tttattgctc gataaattta
     2881 ttgctatcca aacagaaaat actcaaatat taagcgagta tgtctacaca aataatcaaa
     2941 tcattgaaat gcttatcgga ggaatgatat aaccatgaga aaaacttctg tccaaaaaca
     3001 tgttactttg taccctagtc atgttgacgt tttagattcc atcatcgaaa aaaatccaga
     3061 tatcaataac tactcggaag ccattcgctt tttatgtatg agttatgaac ccgataccaa
     3121 cacgaccact accagcaatc aggcaaaact aaatgctatg ggaaaagaaa tttccatcat
     3181 gactgaaatc atcgcagaat ttgctgattt atatctaaaa
//
LOCUS       DARDHU010000167         3215 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000167 DARDHU010000000
VERSION     DARDHU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.172"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            588..1004
                     /locus_tag="SDD03_002752"
     CDS             588..1004
                     /locus_tag="SDD03_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG4305155.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1018..1584
                     /locus_tag="SDD03_002753"
     CDS             1018..1584
                     /locus_tag="SDD03_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG4305156.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1607..2593
                     /locus_tag="SDD03_002754"
     CDS             1607..2593
                     /locus_tag="SDD03_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG4305157.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2748..>3215)
                     /gene="scm"
                     /locus_tag="SDD03_002755"
     CDS             complement(2748..>3215)
                     /gene="scm"
                     /locus_tag="SDD03_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG4305158.1"
                     /translation="TSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          969 a          602 c          682 g          962 t
ORIGIN      
        1 ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa
       61 ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg
      121 gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg
      181 agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa
      241 gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt
      301 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat
      361 tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata
      421 aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag
      481 ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt
      541 ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag
      601 aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg
      661 ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa
      721 aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag
      781 aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag
      841 atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag
      901 gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag
      961 tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg
     1021 agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt
     1081 ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta
     1141 gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag cttcttacta
     1201 ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac attcctttat
     1261 agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca
     1321 aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag
     1381 cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt
     1441 tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga
     1501 catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg
     1561 agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc
     1621 aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga
     1681 agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa cgatccatgc
     1741 cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca
     1801 agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact
     1861 gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag
     1921 tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa
     1981 actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga
     2041 aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt
     2101 aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc
     2161 tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg
     2221 actttctatc gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag
     2281 ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt
     2341 gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc
     2401 aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga
     2461 agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt
     2521 acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga
     2581 tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta
     2641 cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag
     2701 agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat
     2761 ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt
     2821 gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca
     2881 ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt
     2941 gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg
     3001 cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt
     3061 gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg
     3121 gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg
     3181 gtagatgtat ccgtactgtc gcttgaagct gacgt
//
LOCUS       DARDHU010000168         3163 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000168 DARDHU010000000
VERSION     DARDHU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.173"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..406
                     /locus_tag="SDD03_002756"
     CDS             <1..406
                     /locus_tag="SDD03_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4305159.1"
                     /translation="EEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEG
                     VPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSE
                     RSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            412..1434
                     /locus_tag="SDD03_002757"
     CDS             412..1434
                     /locus_tag="SDD03_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG4305160.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1456..2511
                     /locus_tag="SDD03_002758"
     CDS             1456..2511
                     /locus_tag="SDD03_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4305161.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         1273 a          322 c          579 g          989 t
ORIGIN      
        1 ggaagaagaa ataaaaaatt atgcaaagaa aagtggcata gatgatgata ttaaattttt
       61 aggaaataga aatgatatag aaaaatttta tcaagcattt gacgtattaa tgttgccatc
      121 attatttgaa ggggttccgt tagtgggtat tgaagctcaa tttgcagatt taccgtgttt
      181 cttttcggag aaagttccta cagaagttgc tttcagtgat aagaccaatt tcataagttt
      241 aaatcaatcg gcaaaagaat gggcgagaga gattgcaaat gtagatatct ctcatcgaga
      301 ttcagaacgt tcgattttaa taaaggcaga ttataatatt caaacagcat acaaaatctt
      361 agaatcaaaa tattatgagt tatttgaatt aatacaaaga ggttaaagaa tatgacacag
      421 aaacattcaa gtataaaaag aattatacat cgcatatccg aagaattgaa tgcaacaaga
      481 cgaattgcaa agataacatc ttggaaggaa gcatttgcca cgttacaagc taaaattgat
      541 attcaagtca tgaatcgtaa tggatataat gaaagtaaaa aacagaagga acatttatta
      601 aaaaaacatg aggtaatgct gaagtattat gaaaagactt tttcagattt tttgaaaaca
      661 tatgattata gtcaaaatca aataattaat caatcaaaat caccatattc taactgcatt
      721 tgggtttgtt ggtggcaggg attggatcaa tcgccggaaa ttgtaaaagc ttgtgtaaaa
      781 tcaataaaac gaaatgcagg gaaacatact gttataattt taacagaaga taattataga
      841 caatatgttg atataccaaa atgggtggaa gaaaagaaaa acaaaggaat cattacgaga
      901 acaaattatt ctgatttatt aagattatct ttgctagcaa aacatggagg tttgtggtta
      961 gacgccacat tttattgtac aaaaccaata ccagatgagt attttgatat cgatatttgg
     1021 tctattagga ggcctgatta tgctcacgca tccgtagcat caggatattt tgcgggttat
     1081 tcattagcat gtaatcaaga gtctagatgg atgtttgcta taattagaga ctttttttta
     1141 aattattgga aaaataatga tatgatggtg gattatttga tgatagatta tatgattgtt
     1201 ttagcacaga gaataaatca tgaaatagca cagacatttg ataaaatacc tagtaacaat
     1261 ccattatgtg acgaattgat taaaaattta aataatatat atgatgaaaa agtatggaac
     1321 ttattaaaga aagatacttt actgtttaag ttgacatgga aacaatcttt taaaaagcaa
     1381 aaagatgata aagttacttt ttatgggatg ttggtagaaa acaaattatt ataaatatta
     1441 gaggtataaa aagttatgaa acacaaggta agtgtgatag taccaattta caatgttgaa
     1501 aaatatttaa gaaaatgtgt ggatagttta ttgagtcaga cattaaaaga gatagaaatc
     1561 gtattagttg atgatggttc gccagatgct tcgggggaaa tagcagatga gtatcaaaaa
     1621 aaatattcta atgtaaaaac aatacataga gaaaatgggg ggttaggacc ggcaagaaat
     1681 acaggaattg agaatgcgac aggagaatat gtggcttttc ttgattcaga tgattgggta
     1741 gaaagtgata tgtatgaaaa attatactta gttgcgtcca aagaaaatgc tgatatagta
     1801 gttagtggac attgtgattt tgcgaatgac aaagctatgg tgaaaaagca acatcctttg
     1861 agtgggaatg tatatgatac gcctaatagt attaaaaatg tacgaaaaaa tttatttgga
     1921 catagtccaa aagatcaaga agtagaggcc ttcccgatgt cagtatgtat gtctttgtat
     1981 aataaaaatc tgctagataa atatagacta agatttgaaa aaattttatc tgaagataca
     2041 atttttaact tgaatgcata tgatgtcgca aataaaattg cttttacaga atatacagat
     2101 tattgttata gaaaagaaga acaagagtct attacaaaaa gtttatctcc aaataagcag
     2161 acggaattta aagaattttt aataactcta aaagctatag catcgaaaga agatttagaa
     2221 tgtcaaatca gagcaaaacg tatggcaatt gattattgta ggctttatgt gggcttagtt
     2281 gatgatgcaa atttgggaat taaagagaaa agaaagtata taaaaaaatt tattgaagat
     2341 gagggattaa ttcaatgttg gaagggatat cctacaaata cgctaccatt tcaacaaaaa
     2401 ttttttcata aaatgcttga atgtaagtgt tatacaatat tgttgttgtt aagtagaatt
     2461 agaaagataa ttaaaaatat gaccagaaaa aaattgttta taatattata aactaaaagt
     2521 atatcaaata atagaaatta acaagttaca aaaaaatatt ataaagaata atattttcta
     2581 aaaagtaaac tatagtaatg tggattatca ccttccaaca gtatataaaa tgagcgtaaa
     2641 aacggctatt tttgccttaa caacgatttt tttgtataca aatacgatat gctcattaat
     2701 tattggaatt tgtagtgctg tttaaattaa tgaaggtgta attatatgag tgatcatttt
     2761 gaaaaagttt gttgagaaga tgtgcttcta gtcatgtgat gggagaaaat aatttaactg
     2821 ttagagtgaa tagttaaatt attctggtta tatgaaaaaa gtagtttcta aaataatctt
     2881 gcagggatta acttaaagaa attgagagaa tagtttcttt gaaatttcta tatacaatga
     2941 gaagctgaaa tttactttta tttgatgact ggaaatagaa attaaatata ttgtattgtt
     3001 acattaaaga aaacttgtat ggaaaaatca gtacaatacc attatttaat gttcttcact
     3061 taaagcgcaa aaaatatcag tttattaaac tcgtatatag aacaaatata tgtagacggg
     3121 gcgaaacgat gagtgtaaaa aaaatgctat ttatggtaag tac
//
LOCUS       DARDHU010000169         3099 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000169 DARDHU010000000
VERSION     DARDHU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.174"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(14..706)
                     /locus_tag="SDD03_002759"
     CDS             complement(14..706)
                     /locus_tag="SDD03_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4305162.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="SDD03_002760"
     CDS             complement(1143..1712)
                     /locus_tag="SDD03_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4305163.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="SDD03_002761"
     CDS             complement(1786..2109)
                     /locus_tag="SDD03_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305164.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="SDD03_002762"
     CDS             complement(2263..3075)
                     /locus_tag="SDD03_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305165.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          853 a          626 c          545 g         1075 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttcta
//
LOCUS       DARDHU010000170         3049 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000170 DARDHU010000000
VERSION     DARDHU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.175"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..349)
                     /locus_tag="SDD03_002763"
                     /pseudo
     CDS             complement(1..349)
                     /locus_tag="SDD03_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(609..1157)
                     /locus_tag="SDD03_002764"
     CDS             complement(609..1157)
                     /locus_tag="SDD03_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305166.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1158..2015)
                     /locus_tag="SDD03_002765"
     CDS             complement(1158..2015)
                     /locus_tag="SDD03_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4305167.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2301..2587)
                     /locus_tag="SDD03_002766"
                     /pseudo
     CDS             complement(2301..2587)
                     /locus_tag="SDD03_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2577..2906)
                     /locus_tag="SDD03_002767"
     CDS             complement(2577..2906)
                     /locus_tag="SDD03_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4305168.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          890 a          620 c          384 g         1155 t
ORIGIN      
        1 gttcttactg cctcttccac actaatttct tcgttcaatt tcactaataa tacttgatct
       61 cgcttattat aatcgctttc atttatccta agagtcaaat ctttttccac cataactttt
      121 acacccaata aattttgcgg tttatttttc tctgttgaaa ttctttgtgt agcctttaaa
      181 ctgacaatta gctcattggg tacatactta tttgagagtc atctaattgg ctcataatcc
      241 tttctttttg gttagtatct gcagttactt gtttagtatg agccatgaac ccaaacatac
      301 tcaccccaac aaccaatcct aaatatattt tctttttaaa ctttttcatc tacatctcct
      361 cctaatagtt ttattttaga gttagctcta ttacttattt actaactcaa caacagatta
      421 tcatacactt gttaatgaaa tcacaatatc tcattaaaac acaccctaaa atcataaccg
      481 ttcctatgct cattttttgg attttttaat cacgtaacca gaaaaataaa tcccctctat
      541 ttcacgcaaa tcaaagaaga actaagtaac taccgtttaa aggtagttac ttagttcttc
      601 tttgattatt atctaacttt attattcatt ccccaccctt tgcgcagtga gtcttcggta
      661 tcttgtttta tctgactggt agttcttatt atttctccac gatcatctaa ttctggaaca
      721 attggatatt tttctctgaa caagtctagt tctttcacca cgtcattaaa caaatcttgc
      781 ttatcttctt gcgtaagatt ctgtttaata tactcatcta aagcttttct aagcaattca
      841 tctcttgtca tattagtctc atacttttct actaaaactc tggaagtcgc atttaacaaa
      901 gtcgacagtt ttaaaggatg cttggtggaa taaaccttat caaactttct gattggtgcg
      961 ccaacttttt ttgttcttcc tctactctgt ctttcaacat tggaggaagt ttttatttct
     1021 tgaacttggt gcacttctaa ttgcttattt tcagatggtg taattgcttg ctgctttttc
     1081 tttgcttctg cttctttttt tattttggcg agtcctttgt tgttcccaaa aatatcaagt
     1141 cctttgatgt cagccaatta ctccacctcc gcataccaat tctcgtgttc ttccaattct
     1201 ttcaaaatac tgataaataa ttcgtggctt ttgcgatccc attgttttaa gagacctttc
     1261 tttgtcttct ccatcgtaat acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt
     1321 acaacatttt taatcaaatg ttccccataa atctcttggg cttgtttata catttcctga
     1381 tctactgcgt caccgctttc caacatgaaa ggaataacac cagcaacata taaatttgta
     1441 tcaaattctt tcacaaaaaa gtccatatac tcaagatatt gcatcgcatt tctcatcgat
     1501 tttacttggg tctgtaagac gacgattgcc caatccgcaa tgtacatcgc ggatttacta
     1561 tatacagaaa tagaaggtgg gacatctaaa tagatgacgt caaactcatc aaataatggt
     1621 tctagctttt ctttcatata tttgaattgg gataatgaat cattatattg gtctttgatc
     1681 caatcatcat acatttcaaa ggacatgtct gctggaagta tatagaggtt attgattact
     1741 ttatagacag cctctttagg tggaagattg tttatcaaca cgtcagtcac agtattttcg
     1801 taaacaatcg gttcaccttc tccgcctcct tcttcgttag tgaagttcat atgcgttaag
     1861 ttcatcacat cagaagtatt tccttgcata tccaagtcaa ttatcaaggt tctttttcct
     1921 tcattaactg ctgattcaaa ccctaacatt tgcgcgcacg tacttttacc tacgccacct
     1981 ttaaaattac cgattacata tttcttcgct accacgttcc tacctctttt cttttagcaa
     2041 tattcatttg atactattat aacatattta tttttagtta ctaccaaaaa aaagatatat
     2101 atgtactacc ttataattaa taagtaaaag gtagtacata aataaggaaa agctgaaata
     2161 tattgattta tagctttcct caaagtaatg acgttattta atttatcgag aagcagagaa
     2221 tactcgcgac tttagtcatg tagacgaatc agattttcga taaaaaagaa gccgtccttc
     2281 ttaagaacga caacaaatca ctagattaat tttttcaact gttcataaaa gttttcatgc
     2341 gttccacata acaatactgg aataaccttg ttttctataa tctcgtaagc tactctatat
     2401 gttgtgcccg catatttgaa cccactagac caaatacctg ataaatcccc agttttttgt
     2461 tcacccttat atggatcaat cgcaatttca tcataaatta aattcaaaaa tttttctttc
     2521 aatggcttct cttttagctt tttaaatact taatgacatg ttttgaagga acgatttcat
     2581 aagccaattt ctgttgccaa ctcctcttta gtcattactt tagaattagt aagtgtatct
     2641 tcagcaatat ctctaaagga agaatgcatt ttgttttttc tattcttaaa ttctacaaga
     2701 atctcatctt tatcgtagcc ttctgcaatt atatcagaca aaatatcttc gctaaaatca
     2761 taaaattctt tttgtggttc aacaaattca taaagaatac cattctctac caactttgct
     2821 tcaacttcga ctccattagg aacattaaaa tctttaggta ctgtcaacat gattgaattg
     2881 ccttgttttc ttgtttttgt aatcatttaa atccctcatt tctaacagta tgtaattact
     2941 tagtaaatac attataccac gagataatgt atttacaagt aattactgtg actcgtaatg
     3001 cgtccttgct gaataaattt caaccacgtg ttcatcttca tctacctta
//
LOCUS       DARDHU010000171         3039 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000171 DARDHU010000000
VERSION     DARDHU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.176"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..431
                     /locus_tag="SDD03_002768"
     CDS             <1..431
                     /locus_tag="SDD03_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305169.1"
                     /translation="DGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILN
                     SSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDK
                     KETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            418..1839
                     /locus_tag="SDD03_002769"
     CDS             418..1839
                     /locus_tag="SDD03_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG4305170.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1852..2859
                     /locus_tag="SDD03_002770"
     CDS             1852..2859
                     /locus_tag="SDD03_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4305171.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2871..>3039
                     /locus_tag="SDD03_002771"
     CDS             2871..>3039
                     /locus_tag="SDD03_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG4305172.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAK"
BASE COUNT         1077 a          389 c          510 g         1063 t
ORIGIN      
        1 ctgatggtta tggcatagat gaaacagata ttttcgctaa agtaaatagt atattttctg
       61 gaaaaaaaga aactttaatt aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa
      121 aaaacatatt gaattccagt ttaccgtttg aagtcttgct acttgaaaat gacttctctt
      181 caacactttt gattagtcct atatctacaa tttctttttc accatctatg atgtttgaaa
      241 agacgatcaa ctctattatg ttggctaaat taattttaaa tgaatataca gcattatctg
      301 aagataaaaa agaaacattg acaaatattg ttcttttctg tgaacattat aaccgtctat
      361 cagaagaaaa aattttactc ccagaaaact gggcagaatt aagaaagttg gtagagtatg
      421 ggattattta acacactttc caagtcaaaa ttagttaagt ccagtttctg gtatactata
      481 gggaattttt ttattaaggg aatttcattc ataactattc ctatttttgt aaatattact
      541 actcaagcag aatatggttt gattaataat ttcacttcat ttgtatcaat tttttcgatt
      601 cttgttgggc taagcctaaa ttcggcaatt aataatgcaa gttttgacta taaagatgac
      661 ataaatgaat ttatgtcatc ggtactttct ctagctacac tttcgttaat tgtgttctta
      721 ttaataggga acatatattt tttgttttca aatgcttttt ttgatatgaa ccaacttgta
      781 tttaatttga tgttattaca aagttttgct agttttgtaa ttagttatat ccagtcttat
      841 tttacgatta actttaacca ttttaaatat ttgattttat cttttatatc tacttttttg
      901 aatattgcaa tctctgtagc tttgatgcag acattatatt ttgatgataa atatgttggc
      961 agagtagtcg gcggaacact gtcatttgtg ttgataggtt tagtaattta tttaagaata
     1021 atgataaagg gtgctcattt ttttgataaa aaacagtgga gatacgcttt aaaaatctct
     1081 ttgccgctaa tcccacattc cttagcaaat atagttttag cacaattcga tagaattatg
     1141 attaattctt atttaggtcc tgctgcgggg ggaatatatt cctacattag taacataggt
     1201 attattctaa gcgtaatatg gatgtctact aataacgcct gggttccatg gttctatggt
     1261 gagatgagta aaaataatga aaaaaaagtg aagaaagtta gtaactatta tttagtagtc
     1321 tttactttga ttacgatgat tgtaatggta gtttcgattg atttagcaaa atttatggct
     1381 ccgaaagact attattctgg gttatcttta gtggttccga taaccttagg ctatttcttt
     1441 cagtttttat atagtttacc agttaatgct gaattttatg agaaaaaaac atcgtttatt
     1501 gcgattggta cagtagcaag tgctatagtt aatatactat taaacatgat tttcatacct
     1561 aggttaggaa taattgctgc tggatacaca accgtattta cgtatttttt actttttatt
     1621 tttcattata atatcgctaa aaaaattacg aataaacaat tatttgacac aaaaaaaata
     1681 tgtatcataa taatttttgt atcaattttt tctttgatac ttcatatttt aatcaatcaa
     1741 atgttgattc gttacgtttt gacatcatta ttgataatta gtttagttat atatttttta
     1801 aagattaaga atcgaattat agatgactat aattcatagg aggagatata agtgtttaaa
     1861 ggaaaaacaa tattagtaac tggcggaact ggttccattg gaagtgaaat tgttcgtcag
     1921 cttttaaaat atgaaccaaa agctatcaga atttatagta gaggtgaaga aaaacaattt
     1981 tatatgcaac aagagctctc aaatcacact aatttgagat ttttaattgg tgatatcaga
     2041 gactctcaaa ggattgatta tgcatgtcgt gatgtggata ttatttttca tgctgccgca
     2101 atgaaacacg ttccagcaag tgaatataat cctatggaag ctgtaaaaac aaatgtgatg
     2161 ggaactcaaa atgtaataga tgcagcgatc aaaaataatg taaaacattt tgtaggtatc
     2221 agtacagata aagtagtgag tcctactaac accatgggtg cgacaaagtt attgagtgaa
     2281 aaattaataa tagctgccaa tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta
     2341 aggtttggaa atgtcatggg gtcaagtgga tctgttattc ctctttttag taaacaaata
     2401 aaaaacaata aagatattac tttgacagat gttgatatga cacgatttat gatgtcaata
     2461 ccggatgctg caagattagt tctgtctgct gctatgaatt caaaatttgg agaaacatat
     2521 gtcttaaaaa tgccggcaat caatatttta actttagctc aatcattaat aaaaatatat
     2581 aatgatacac atgatgaaaa atatagaggt gaaataaaag ttgtaggtat caggccagga
     2641 gaaaaattat atgaagagct aatgactaca gacgaaattg aacgctcata tgaaaatgat
     2701 aaaatgtata tcattccacc acactatcat gctactgacg aactgtacaa agattatgaa
     2761 aaaattaaaa tcactgcata ctcttcagat gcaacagaat taatgagtgg acaggaattt
     2821 attagaatcg caaaagagaa taatgtcgtg acttggtaag gagttttatg atgaaatcag
     2881 aagtatatgt aattgcagaa attggtgtaa accataatgg aagtctagat ttagctttag
     2941 aaagtattga taaagctaaa gaatgtggag ctaacgcggt taaatttcaa acatttaaaa
     3001 cgaataagct agtttccaaa aaggctccaa tggcaaaat
//
LOCUS       DARDHU010000172         3024 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000172 DARDHU010000000
VERSION     DARDHU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.177"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1243
                     /locus_tag="SDD03_002772"
     CDS             <1..1243
                     /locus_tag="SDD03_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4305173.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="SDD03_002773"
     CDS             1256..1888
                     /locus_tag="SDD03_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4305174.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="SDD03_002774"
     CDS             2093..2320
                     /locus_tag="SDD03_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG4305175.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="SDD03_002775"
     CDS             2389..2655
                     /locus_tag="SDD03_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4305176.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="SDD03_002776"
     CDS             2645..2914
                     /locus_tag="SDD03_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4305177.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DARDHU010000173         2916 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000173 DARDHU010000000
VERSION     DARDHU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.179"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..1371)
                     /locus_tag="SDD03_002777"
     CDS             complement(61..1371)
                     /locus_tag="SDD03_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG4305178.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="SDD03_002778"
     CDS             complement(1383..2801)
                     /locus_tag="SDD03_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4305179.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          914 a          606 c          453 g          943 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaa
//
LOCUS       DARDHU010000174         2914 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000174 DARDHU010000000
VERSION     DARDHU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.180"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1364)
                     /locus_tag="SDD03_002779"
     CDS             complement(45..1364)
                     /locus_tag="SDD03_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4305180.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="SDD03_002780"
     CDS             complement(1361..2014)
                     /locus_tag="SDD03_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4305181.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2914
                     /locus_tag="SDD03_002781"
     CDS             2302..>2914
                     /locus_tag="SDD03_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305182.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          955 a          474 c          321 g         1164 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgc
//
LOCUS       DARDHU010000175         2826 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000175 DARDHU010000000
VERSION     DARDHU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.181"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            798..1052
                     /locus_tag="SDD03_002782"
                     /pseudo
     CDS             798..1052
                     /locus_tag="SDD03_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="SDD03_002783"
     CDS             1122..1634
                     /locus_tag="SDD03_002783"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305183.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="SDD03_002784"
     CDS             1631..2668
                     /locus_tag="SDD03_002784"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4305184.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DARDHU010000176         2758 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000176 DARDHU010000000
VERSION     DARDHU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.182"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(261..545)
                     /locus_tag="SDD03_002785"
     CDS             complement(261..545)
                     /locus_tag="SDD03_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305185.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(566..1936)
                     /locus_tag="SDD03_002786"
     CDS             complement(566..1936)
                     /locus_tag="SDD03_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG4305186.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            2155..>2758
                     /locus_tag="SDD03_002787"
     CDS             2155..>2758
                     /locus_tag="SDD03_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG4305187.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILG"
BASE COUNT          853 a          562 c          418 g          925 t
ORIGIN      
        1 tatgtgaaaa tcgaatcttg agatagctat tccccttgag atattcactg atacttctac
       61 tcaaatcatt taaattttta taattatttg tacggaacgt atatatatca acatctattt
      121 agacgttgtc ggtgatacaa tgtcattttt cccgtgtact atgtcattta tctctatact
      181 ttgtcatttt tctttttggg caatcaaaaa aggcaacaaa ccaattatga tttgctacct
      241 tctccgttta aaaacttatc ttagccccaa aatggaatcc aatttttaac ttcttttgcc
      301 aatgcatacg ccttagcaag acctgagttt tcaactaaat atttttcccc ttccagagaa
      361 acactccccc agatatgaac agtgttatcc gcccaaacca tggattctag taagccattg
      421 cgcgaaagaa atttagtttg ttctaaaata tcttcttctg gaacttgccc tttaagctca
      481 ataaaaggat tctttttttc atcacgcaat ttaagaattg catatcttag tttgttctta
      541 tccaacctaa caccacctct atgctctatt tcttatttct tagcgaatca ttaatactta
      601 ttccgttatc atatttccag tagtcccaac cattccggct accccctaat agcagagctg
      661 ctgcattcga aacagatgag gcagtgtaat cttgaacaaa ttgtagtccc ccaattgttt
      721 cttcaatgat tccattatcc aacagctctt ggcgagtttc atctagcgaa tcagatgcaa
      781 tatctttaat cttagaataa atttctgaac ctgaatgtat aatcacggtc tcgtcttcgt
      841 tcaaataccc agttgctttt attccttgcg cctctaagaa aatttcccta ttcccatcgt
      901 gttcatagtc gcccgacgat ctcaaatagg gatacatagt aatcagttca ttgattagag
      961 cttcacttct ggcagaaata tccgatgatg tccactcagt caattcgtac aaatctttat
     1021 tcatacgaat atgaagggta tcttcaaaga ttttcttttt ggtctcaaag tctttatttc
     1081 ccgcggccga attatcagga tttgtaacga gtgttaaatt accaatggta tttactaatc
     1141 ctgtgtattc ttcccccgat gtaccagcct tctcttccca atatgaagta ctagtctgtg
     1201 gcatgatgtg ttcaatggac aagttactca tatcaagtgt tgctgaattt tctcgattct
     1261 ctattttttc aagtaaccaa cgggccaatc tcatcgcata agcattggca ttcaatagat
     1321 tagatttcaa tgccttgtca gatggaagtg ccatattatt tgattgattt ctagtaacaa
     1381 gaacataaat tacaatatca acgatatttt catatccatt agctttagcg tacttcctaa
     1441 cacttttcaa atatgaagga aatgcttttg acactctgct tgtatcatcc ccattaaaat
     1501 atctcctaag ctgataatca ttcactacct taatcgcatc aatatattgg tcttcagtga
     1561 taaattggtc atgatagtac cattcagata atcccaaaac aaaaggtgca ggcatcatac
     1621 tttccatact ttggaaatcg ctcaatactt ctgagtattt tccttcaggt ttgttatagt
     1681 aaagtctcgc aaaataacca gcatatcgca ctaatttttg gagcaattcg gcatcacttg
     1741 atacatcgcg ctctttatgc caatagtctt tgaatgcttg ataaagaata tgctttgctg
     1801 aatactcccc agttattgca gcaaggtagt atctgaaaaa gtcttccatt cctttagaat
     1861 tatcaaagac ttcttccaac ctacgccaat atttagtata aagcgtagtc tgctcttcat
     1921 tagatttatc catcatgatg aagtttcgaa tcaaatccga ggcagtcaat ctttcaccgg
     1981 tagaattgat actttcaaaa atttgctgtg cattatcacg gtcatctaat ttaatataaa
     2041 caatacgcgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa
     2101 tcaacaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact acatatgcgt
     2161 tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga aattatgaga
     2221 actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg aagaaatact
     2281 gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg cataattaat
     2341 ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt tcttaatggt
     2401 gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta cactatcaag
     2461 attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg aattatagat
     2521 agttttccta gtcaattatg taaaccagtt agaaatagac aagcaaaact attgaatcaa
     2581 atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct taaaattcat
     2641 atgatcgtta ctaagactgg ctttccgatt acttactcaa tcacgaatcc aggtgtccat
     2701 gacgtgaaag tgttagagac tttatcggaa gaagcgaatc ttcccaatat cctagggg
//
LOCUS       DARDHU010000177         2739 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000177 DARDHU010000000
VERSION     DARDHU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.183"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="SDD03_002788"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="SDD03_002788"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG4305188.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DARDHU010000178         2673 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000178 DARDHU010000000
VERSION     DARDHU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.184"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..323
                     /locus_tag="SDD03_002789"
     CDS             <1..323
                     /locus_tag="SDD03_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4305189.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            737..1555
                     /locus_tag="SDD03_002790"
     CDS             737..1555
                     /locus_tag="SDD03_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4305190.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1628..1987)
                     /locus_tag="SDD03_002791"
     CDS             complement(1628..1987)
                     /locus_tag="SDD03_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4305191.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          372 c          471 g          905 t
ORIGIN      
        1 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
       61 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      121 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      181 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      241 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      301 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      361 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      421 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
      481 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
      541 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
      601 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
      661 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
      721 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
      781 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
      841 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
      901 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
      961 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1021 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1081 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1141 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1201 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1261 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1321 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1381 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1441 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     1501 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     1561 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     1621 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     1681 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     1741 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     1801 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     1861 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     1921 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     1981 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2041 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2101 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2161 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2221 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2281 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga
     2341 aaattcaaag gattactggt ttttctaaaa tagtttagtt taaatacata agtatcctcc
     2401 gacactgtat tagttcataa tcatctaaat acaaaatttt caaataaaaa tatttcaagc
     2461 tctatcgttg acgaatgtaa acgatactta taaagtaata gaaattgatt acatttgtaa
     2521 acttaaaatg gttaatttat ctcacataaa actaagaata aaataatgat taaaagtggt
     2581 tagtttttat acgatttagc ctatcggtaa aaatgaatta gagagtgtaa aatattttgt
     2641 gtaaatgaaa aaatccatac aaaaaaggaa gtc
//
LOCUS       DARDHU010000179         2488 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000179 DARDHU010000000
VERSION     DARDHU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.185"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1023
                     /locus_tag="SDD03_002792"
     CDS             <1..1023
                     /locus_tag="SDD03_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4305192.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..>2488
                     /locus_tag="SDD03_002793"
     CDS             1157..>2488
                     /locus_tag="SDD03_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4305193.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLN"
BASE COUNT          982 a          317 c          474 g          715 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaac
//
LOCUS       DARDHU010000180         2412 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000180 DARDHU010000000
VERSION     DARDHU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.186"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..515
                     /locus_tag="SDD03_002794"
     CDS             123..515
                     /locus_tag="SDD03_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305194.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="SDD03_002795"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="SDD03_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            845..1054
                     /locus_tag="SDD03_002796"
     CDS             845..1054
                     /locus_tag="SDD03_002796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305195.1"
                     /translation="MICLLKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDV
                     LLRFNGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="SDD03_002797"
     CDS             complement(1097..1672)
                     /locus_tag="SDD03_002797"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG4305196.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="SDD03_002798"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="SDD03_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          765 a          413 c          377 g          857 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tc
//
LOCUS       DARDHU010000181         2408 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000181 DARDHU010000000
VERSION     DARDHU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.187"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..464)
                     /locus_tag="SDD03_002799"
     CDS             complement(243..464)
                     /locus_tag="SDD03_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305197.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="SDD03_002800"
     CDS             complement(562..807)
                     /locus_tag="SDD03_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305198.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="SDD03_002801"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="SDD03_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="SDD03_002802"
     CDS             complement(2031..>2408)
                     /locus_tag="SDD03_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG4305199.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DARDHU010000182         2407 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000182 DARDHU010000000
VERSION     DARDHU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.188"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SDD03_002803"
     CDS             5..1150
                     /locus_tag="SDD03_002803"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG4305200.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SDD03_002804"
     CDS             1321..1779
                     /locus_tag="SDD03_002804"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG4305201.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SDD03_002805"
     CDS             1938..>2407
                     /locus_tag="SDD03_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG4305202.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARDHU010000183         2400 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000183 DARDHU010000000
VERSION     DARDHU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.189"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..2069
                     /locus_tag="SDD03_002806"
     CDS             207..2069
                     /locus_tag="SDD03_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4305203.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="SDD03_002807"
     CDS             2069..>2400
                     /locus_tag="SDD03_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4305204.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DARDHU010000184         2390 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000184 DARDHU010000000
VERSION     DARDHU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.190"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(4..249)
                     /locus_tag="SDD03_002808"
     CDS             complement(4..249)
                     /locus_tag="SDD03_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305205.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="SDD03_002809"
     CDS             complement(266..1117)
                     /locus_tag="SDD03_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4305206.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="SDD03_002810"
     CDS             1283..2287
                     /locus_tag="SDD03_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG4305207.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          764 a          403 c          483 g          740 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga
//
LOCUS       DARDHU010000185         2268 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000185 DARDHU010000000
VERSION     DARDHU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.191"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..1104)
                     /locus_tag="SDD03_002811"
     CDS             complement(205..1104)
                     /locus_tag="SDD03_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG4305208.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="SDD03_002812"
     CDS             complement(1114..1470)
                     /locus_tag="SDD03_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4305209.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="SDD03_002813"
     CDS             1825..2121
                     /locus_tag="SDD03_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG4305210.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          707 a          430 c          390 g          741 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgg
//
LOCUS       DARDHU010000186         2255 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000186 DARDHU010000000
VERSION     DARDHU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.192"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(284..1624)
                     /gene="glnA"
                     /locus_tag="SDD03_002814"
     CDS             complement(284..1624)
                     /gene="glnA"
                     /locus_tag="SDD03_002814"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG4305211.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1652..2044)
                     /locus_tag="SDD03_002815"
     CDS             complement(1652..2044)
                     /locus_tag="SDD03_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4305212.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          642 a          499 c          381 g          733 t
ORIGIN      
        1 cgtctttttc aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt
       61 tcagccaatt caaaaacaag aaaagctgtt tttgcttggc tattcttatt acttgttgca
      121 gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
      181 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt agtttctcta atactcaggt
      241 caaaacgctg atttcacagc ctctaattca cggtattcct gttttaataa agttctagat
      301 attgttctct ttcccactcg gaaacagttt gtctgaatgc cgcccattcg agacgttttg
      361 cttcaacaaa atttgagaag atatgttgtc ctaatgcgtc aatcattaca ttatccttac
      421 gcaattcttt gatagcatta tggatagttg aaggtaagtc ctggatatgt gcagcttctc
      481 tttcttcctc atccattaca taaatgttac ggtcaacagc tgctggcggt gtgatctgat
      541 tacggatacc gtctagtcct gcttgcaaca atactgccat tgccaagtat gggtttgcag
      601 atggatcaac tgaccgcaat tccaaacgtg tggataaacc gcgagattct ggtacacgta
      661 ctaatggcga acgattacgg ccactccatg cgacataaac aggcgcttca taacctggaa
      721 cgagacgttt ataagaattg actgttggat tgcaaacggc tgtgtaggca cgagcatgtt
      781 tcagcaaacc acctaagaaa tgataagctg tttcacttag ttccaattca ccgtctttat
      841 catagaaaac atttccttca tcattgaaca aagacatatt gcaatgcata ccagaaccat
      901 tgatcccaaa taacggtttc ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg
      961 ttttgacaac gagcttgaag gtttggatat tatcacaagc atctaccaca tctgcatatt
     1021 taaagtcaat ttcatgctgt cctggcgcta cttcatgatg tgaagcttcc acttcaaagc
     1081 ctagactttc taattccaac acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg
     1141 caaaatcaaa ataaccacct ttatcattca aggtcgttgt gatcccaccg tcttcgtcta
     1201 atttaaataa gaagaattct ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc
     1261 gcatatcttt caatgctcgt ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat
     1321 ccggattata gatatcacaa attaaacgag caacttttcc atgagcactt tcccacggga
     1381 agatcatcca agtggagaga tctggataca aatacatatc gctttcttca attcgaacaa
     1441 atccttcaat cgaagaaccg tcaaacatca ttttattttc taatactttg tcgatctggc
     1501 tcactggaac ttctacattt ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa
     1561 atcgtacgtt ttcttcctca atgattcgtt tgatttcttc acctgttatc tgtttcttta
     1621 ccatggtatc cctcttcctt ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt
     1681 gcgactgata atgacctgtt ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa
     1741 tttgtcgaac gtcatgatct gtcaacggct tcttgttttt tgcttcttgt tttttcgcct
     1801 gtgccatttc gtatacacgt ttgatcccag ccatattcaa tccctctgaa agatagtctt
     1861 taatctcaag caacatatca atatcattca gcgaatacat acgtcggttt ccttcgcttc
     1921 gttccggatg aactaaatct tgttcttcat aatagcgaat ttgccgagca gacaaatcag
     1981 tcagcttcat aactgtacca attggaaata cggacataga tcggcgcaat tccttttctc
     2041 ccatcagacg tcccctttcg ttttataagt tggctaaagt gtaccaatta aaaaaaatat
     2101 tgtcaactgc atatgttagg ttttctaaca tgagatctct attttttata aaataacaaa
     2161 aaaataagaa cacaaaaaag gtatctagtt gattttcgta ccagatacct tttatttttt
     2221 attatactgt actatccttt attagtgttt ttatt
//
LOCUS       DARDHU010000187         2235 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000187 DARDHU010000000
VERSION     DARDHU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.193"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(34..2118)
                     /gene="cadA"
                     /locus_tag="SDD03_002816"
     CDS             complement(34..2118)
                     /gene="cadA"
                     /locus_tag="SDD03_002816"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG4305213.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          646 a          509 c          355 g          725 t
ORIGIN      
        1 cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt ttacatgcaa
       61 gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa atactgcatc
      121 tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta tcccaatagc
      181 aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga ttgcttctgc
      241 aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg cagcatcgct
      301 tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg catcgttgat
      361 tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct ctaaccgctg
      421 cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt gcttcccgat
      481 atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca caccagcctg
      541 ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa taacaagata
      601 accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt gacttaaagg
      661 aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt gtttgccatc
      721 aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg gtaaaagcgg
      781 acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact gttctgcact
      841 cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct ggatcgaaaa
      901 gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt gcgcaagcaa
      961 ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc cgataccgcc
     1021 aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca ctaaaaatgt
     1081 caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac
     1141 agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat
     1201 gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta ataatttgct
     1261 gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat ttttattgat
     1321 aaatccgctt aatactgttt caccaacata tacagattta gggactgatt ctcctgtaag
     1381 tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg gtacacgttc
     1441 accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca cggtcatcaa
     1501 tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt cgcgaatcga
     1561 ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat aaaatagcat
     1621 cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag caacagacat
     1681 aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct tcttcactac
     1741 atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg gttcattaaa
     1801 atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga acagcgtatt
     1861 tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg ttactttgac
     1921 atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag ccaaatgatc
     1981 ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt ccttgactcc
     2041 attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat ctaaaccttc
     2101 taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca tctcttcata
     2161 actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac
     2221 atctgcatcg gaaaa
//
LOCUS       DARDHU010000188         2160 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000188 DARDHU010000000
VERSION     DARDHU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.194"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..368
                     /locus_tag="SDD03_002817"
     CDS             <1..368
                     /locus_tag="SDD03_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG4305214.1"
                     /translation="FGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFG
                     RVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGK
                     ENWNDLYHANEVLKIAEEK"
     gene            369..1121
                     /locus_tag="SDD03_002818"
     CDS             369..1121
                     /locus_tag="SDD03_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG4305215.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1126..2073
                     /locus_tag="SDD03_002819"
     CDS             1126..2073
                     /locus_tag="SDD03_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG4305216.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          765 a          282 c          418 g          695 t
ORIGIN      
        1 tatttggttt ccctaaatca gtaaaaaaac agtttggtaa atattcgaat cttgaaatta
       61 acagtaatga tttagcaaca tatatattga gatacgatga taaaattata gaaattcatt
      121 tagattattt tggaagagtt cctacaagaa aatttcaatt gtttacttta gatgaatgta
      181 tcaatgttga cttattaaat aataaaataa gttttgaaga aaacattgtt aaagaaatgc
      241 ctgaatctgc taatgatatg tatgagaagg agttgaaata tttctttgaa aacgtagtaa
      301 caggcaaaga aaactggaat gatttatatc atgcaaacga agtattgaaa atagctgagg
      361 agaaataaat ggaaatttta tttactattt gtggtagagc tgggtctaag gggtttaaaa
      421 ataaaaattt gaaatatttg gcaggaaagc ccctagtcca tttcgcttta gcaatgatag
      481 atatctatca acaaaaacat cctgaacata attgcacgac ttgtttgaat acggacagta
      541 atgagttagt agaattgacg actaagttaa atccgtctgt gaaaattgta gaacgtaaac
      601 ctgaattagc tacagatact gtagggaaaa ttgaagtgat acgtgattca ttcttacaac
      661 tagaaagaga agataatata tttgatttaa ttatagattt agatttgact tcacctttac
      721 gtgaagtttc taatcttgaa gaattaatag atgaacatct aaaaaattta gataaagacg
      781 tctacttttc agttgtttct gcaagacgta atccctattt taatatggta aaaagaattg
      841 aaaatgaagt tactatagtt aataaatcaa gatacacatc taggcaacag acacctgaag
      901 tttttgaaat gaatgcctca atgtattctt ttaaacctga atttctaaaa aaaagtaatt
      961 atatttttga tgggcaatgt gggataataa aaatgagaga ttatctaatt ttagatattg
     1021 atagtgaaga agattttaaa tggttagaat ttttgtttcc taaattttta gaagattctt
     1081 cggagcttca agatatctat aattatttat atcggaggta aaaggatgag ctgttatctt
     1141 atcaccggtg gggctggctt cattggttct accttagcca atcatttagg agaagaacat
     1201 gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag aaaatcttga tatgacgaaa
     1261 aatatcactt ttattgaggg agatgtcgct gagaaatcct tgatggaaga gatcatgaaa
     1321 gctaataaat ttgattatat ctttcattta gcagcagttg ctagtgtggc agattcagtt
     1381 gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg tattaatgat tttagaattg
     1441 atccgcaagt atcaacctga tttaaaacgg atcgtgttta gttcttcagc ggctgtttat
     1501 ggggatgaac cgactttacc aaaaaaagaa gagtccgtga ttcgcccatt gacgccttat
     1561 gcggttgata aattcgctgc agaacaatat gtgctagatt attgtcattt atatgatgta
     1621 cccggtagcg cagttcgttt tttcaatgtc tatggtccaa atcaaaatcc gaattctcca
     1681 tacagtggtg tgatttctat tctcgtagat cgctataaga aacaaatcgt aggagaagca
     1741 acttcattta cattatttgg tgatggaagc cagtctagag actttgttta tatagatgat
     1801 gtaatccaag cattactttt agttgcgaat gaggaagctg cactggggaa acaattcaat
     1861 gttggaacgg gtaaagcaac cacacttttg gaattgattc atgcaatcaa tgaaattctc
     1921 ggtgtagaat tgacacttga atacaaagaa gagcgttcgg gtgatatcca tgactctcta
     1981 gcagatatta ctaagatcca gtcaattggc tatcaaccga aatatggtat tttaagtgga
     2041 atgactaatt atttgaaaac tgaaatcaat taaaacatac taagataaga ttagagggta
     2101 aattcaggct ctttgtcaat aaggactgat gagttttgta aaatcaaatc tgggatagaa
//
LOCUS       DARDHU010000189         2105 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000189 DARDHU010000000
VERSION     DARDHU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.195"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SDD03_002820"
     CDS             complement(<1..914)
                     /locus_tag="SDD03_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG4305217.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SDD03_002821"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SDD03_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG4305218.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          709 a          373 c          410 g          613 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaa
//
LOCUS       DARDHU010000190         2065 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000190 DARDHU010000000
VERSION     DARDHU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.196"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SDD03_002822"
     CDS             <1..1008
                     /locus_tag="SDD03_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305219.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SDD03_002823"
     CDS             1025..1174
                     /locus_tag="SDD03_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305220.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SDD03_002824"
     CDS             1171..1617
                     /locus_tag="SDD03_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305221.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SDD03_002825"
     CDS             1619..1756
                     /locus_tag="SDD03_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG4305222.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="SDD03_002826"
     CDS             1794..>2065
                     /locus_tag="SDD03_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305223.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DARDHU010000191         2058 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000191 DARDHU010000000
VERSION     DARDHU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.197"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..337)
                     /locus_tag="SDD03_002827"
     CDS             complement(<1..337)
                     /locus_tag="SDD03_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4305224.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="SDD03_002828"
     CDS             complement(404..559)
                     /locus_tag="SDD03_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4305225.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="SDD03_002829"
     CDS             1176..1397
                     /locus_tag="SDD03_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305226.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="SDD03_002830"
     CDS             1398..1655
                     /locus_tag="SDD03_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305227.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="SDD03_002831"
     CDS             complement(1791..>2058)
                     /locus_tag="SDD03_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4305228.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DARDHU010000192         2057 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000192 DARDHU010000000
VERSION     DARDHU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.198"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..1853)
                     /locus_tag="SDD03_002832"
     CDS             complement(387..1853)
                     /locus_tag="SDD03_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4305229.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          644 a          412 c          341 g          660 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgc
//
LOCUS       DARDHU010000193         2055 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000193 DARDHU010000000
VERSION     DARDHU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.199"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..365
                     /locus_tag="SDD03_002833"
     CDS             <1..365
                     /locus_tag="SDD03_002833"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4305230.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="SDD03_002834"
     CDS             complement(721..1293)
                     /locus_tag="SDD03_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4305231.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /locus_tag="SDD03_002835"
     CDS             complement(1309..1914)
                     /locus_tag="SDD03_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG4305232.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DARDHU010000194         2003 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000194 DARDHU010000000
VERSION     DARDHU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.200"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SDD03_002836"
     CDS             <1..378
                     /locus_tag="SDD03_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG4305233.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SDD03_002837"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SDD03_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG4305234.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SDD03_002838"
     CDS             1859..>2003
                     /locus_tag="SDD03_002838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305235.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARDHU010000195         1991 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000195 DARDHU010000000
VERSION     DARDHU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.201"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="SDD03_002839"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="SDD03_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG4305236.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="SDD03_002840"
     CDS             852..1040
                     /locus_tag="SDD03_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG4305237.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="SDD03_002841"
     CDS             1053..1610
                     /locus_tag="SDD03_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4305238.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="SDD03_002842"
     CDS             1642..1884
                     /locus_tag="SDD03_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4305239.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc c
//
LOCUS       DARDHU010000196         1962 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000196 DARDHU010000000
VERSION     DARDHU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.202"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..272)
                     /locus_tag="SDD03_002843"
     CDS             complement(<1..272)
                     /locus_tag="SDD03_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305240.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="SDD03_002844"
     CDS             complement(309..446)
                     /locus_tag="SDD03_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG4305241.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="SDD03_002845"
     CDS             complement(448..894)
                     /locus_tag="SDD03_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305242.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="SDD03_002846"
     CDS             complement(891..1040)
                     /locus_tag="SDD03_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305243.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..1386)
                     /locus_tag="SDD03_002847"
                     /pseudo
     CDS             complement(1054..1386)
                     /locus_tag="SDD03_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DARDHU010000197         1796 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000197 DARDHU010000000
VERSION     DARDHU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.203"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="SDD03_002848"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="SDD03_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG4305244.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DARDHU010000198         1746 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000198 DARDHU010000000
VERSION     DARDHU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.204"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            17..397
                     /locus_tag="SDD03_002849"
     CDS             17..397
                     /locus_tag="SDD03_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG4305245.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            379..1293
                     /locus_tag="SDD03_002850"
     CDS             379..1293
                     /locus_tag="SDD03_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG4305246.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            1253..1531
                     /locus_tag="SDD03_002851"
     CDS             1253..1531
                     /locus_tag="SDD03_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305247.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            1538..>1746
                     /locus_tag="SDD03_002852"
     CDS             1538..>1746
                     /locus_tag="SDD03_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305248.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISR"
BASE COUNT          656 a          351 c          338 g          401 t
ORIGIN      
        1 tgaaagggtg ggacttatga gcgaacaaaa tcaatatttg gctagcaatc attctaaaaa
       61 atatacctat cgatccgagc caaaacaaat cagttttcga gtgagcgaat ccgaatttgc
      121 aaagctgaag cagtcagctg aagctttgca aatgagtgtg ccggctttcg tgaaagccaa
      181 ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca gacattgctc aggcaatcgc
      241 gcgcgattta gccaaagctg gcgggaatat caatcaaatt gccaagtggt gcaacacaca
      301 ccaacacgac gttgatcctg gcgacgccca acgcttatcc gaaaacctaa aaatcatgca
      361 aaaggagcta caaaagatat ggcaacagtt aaagtaagcc gaaccacttc ttgtagccga
      421 gcgatcaatt acgccgaacc acgagcaacc gtcaaaacag ggataaactg tgacattgat
      481 tatgccaaaa gcgaaatgaa acaaattcgc atgctgtatg gaaaagacga tcgtgtacaa
      541 gcacacttat tgatccagtc ttttcgacca ggagaaatta ccgctgaaaa agccaatcaa
      601 ctaggggcag agtatgcgaa aaaaatagcc cctgaccacc aaatcgcgat ttacacacat
      661 acagacaaag accatattca taaccacatt gtcattaact ccgtcaatct ggaaactggg
      721 aacaaatttc aagcccatgg tcaagcattt ttaaacaaat gttatgacct aaatgacgag
      781 atttgtcttg aacatgactt aacaatcact gaacgaggga agaaacccga aaaacgaacc
      841 atgtctgaaa tcaaattaaa agaaaaaaat gaacctgttt ggaaagacga aattcggttt
      901 gcgattgatc aaacaatgaa aaacaaaaaa actcgaacgt ataaccaatt ttgtgattct
      961 cttaaaacat tcggcattca ttgcttgaat cgtgggaaaa attttactta cgaattgatc
     1021 cacaaaaaga aaaaagtacg atccaacaaa ctaggaaagg attacgaaaa ggagacgatt
     1081 ttacatgagc tggacagacg agaacgagcc tacaatcaac aacgaaccaa cgagctacga
     1141 agagctcgag ctagcattcg cgaaactgca aaagaagtac gaagagaaca acgaactctt
     1201 acgactgaac aaccggaaat tagaccgact cgaagaaacc atgaccaaca cgttgaaaga
     1261 ggagcagact accaacaaga attatcgttc taaaacgtta caagacatag aaaatgaaat
     1321 aaagaaacaa cgtgcttttc ttgcacctta taccaaagaa agaatacaaa ctacaattga
     1381 ttcacaagtc aaaaaattcc accatgcgct attaaaccag cgaaataaag aacagaaaaa
     1441 agtaattatg cagcgatcaa tggatattct tgccccctta tgttgcttaa tcatgacact
     1501 gttagccttt tggctggtat ggggagaata aatagaaatg gcattctatt taccctatct
     1561 actcattttt gccagtattt taggaattat ttggcttagc tataaggctt atcaaacaag
     1621 atattcatta aaaggctcta aaattcgatt taagaggttt ttgttgccag gggttatatt
     1681 tacattatca atcgttttaa gtggtttatt cggcgtttta gagggaaata aacgtatttc
     1741 aagaag
//
LOCUS       DARDHU010000199         1728 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000199 DARDHU010000000
VERSION     DARDHU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.205"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            279..1319
                     /locus_tag="SDD03_002853"
     CDS             279..1319
                     /locus_tag="SDD03_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG4305249.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1568..>1728
                     /locus_tag="SDD03_002854"
     CDS             1568..>1728
                     /locus_tag="SDD03_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4305250.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          611 a          208 c          371 g          538 t
ORIGIN      
        1 cagtttaggg gtatatattt tacatgaaaa tcattatttc attgatggta tgaagtctag
       61 aggcttaata tgggatggga tatttggatt aggcaaaagg tttttagata gggatttttg
      121 gatttggttt ataattgcag ttgtagttgt gtgcataatt tgcatggcta tcgatgttgt
      181 aagagaaaaa tttgaaagac agatttttaa aattaagagt gtaaagaacc tgtgtaaact
      241 tatgaatcat gttataaata tatacccata attaataaat gaggagaaaa gaagtgaaag
      301 ttatatcatt cggagaatta ttattgagac ttgcagcacc cggttattca aagttatttc
      361 aaagagatag tttggagact acattctgcg gaggggaagc taatgtagcg gtatccttag
      421 caatatttgg atatcaatca gaatttataa caaagcttcc ggattcagat gttggatatg
      481 cggcagctaa tgctttgcgt tattttggag taaatgttga taatattata tttggagagg
      541 gacgcatggg actgtattat ttagaaaaag gagcagctca gagaccatct aaagtaattt
      601 atgacagaaa attttctgca atttcgttag ctgatagtga tgaatttaag tggaaggaga
      661 tcctaaacga tgcaaattgg tttcattgga cagggataaa tccagcattg tctgagaatc
      721 tctttgagac gtccttagat gcatgtcagg ctgccaaaaa aagaggtata acaatttctt
      781 gtgatttaaa ttatcgatcc aagctttgga catcagagga agcaaaagaa aagatggaac
      841 agatactacc attggttgat atttgtattt gtaatgaaga agatgcagaa aaagtgctag
      901 gaattaaagc tgagcataca aatattaata gtggagaatt aaataaagat ggctatgtgc
      961 aggtggctag ggagattcac aagaagtttg gatgtaggta tgtagctaca actctgcgta
     1021 gaagttattc ggcaagtaga aatggttgga gtgcaatgtt atacgaagca gaaaaagata
     1081 aagcgtattt ttccacagaa tacaacatac aaattgttga tcgcgttggt ggtggagaca
     1141 gcttttctgc tggtataata tatgcgttat gtgaaaatat gtccggacaa aaggctattg
     1201 aatttgcgac tgcatctagt tgtttaaaac atactataga aggtgatttt aatcgaacta
     1261 caattgagga tgtatataat ttaatgaata gcggtggaaa tggtcgcgtg ttgcgttagt
     1321 caacatataa agataaatca cagtaatttt tatggtgatt atctgtctta gtagtgatta
     1381 gaataacgtg aaaataaatt gttgagcgtt tatactgttt tgaaaaataa atttaacata
     1441 ttgaaaaata caaatatata cgtatattat aaagcgccga ttgtaaaatt aagctagaca
     1501 actaaaacag tcatttgtgc tacactcaaa atagttccga ccaaagaatt ataaggagtg
     1561 agtgcaaata acctatacct atcttacaac ggatgaactt gtaataatag agtcttattt
     1621 gaaaattaac caatctgttg ctaaaactgc gcattgcttg aatcgttcaa gtcaaacgat
     1681 ccataaagta tatctgttct tcaagcaaag aaaatcagcc ttagaata
//
LOCUS       DARDHU010000200         1676 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000200 DARDHU010000000
VERSION     DARDHU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.206"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            316..570
                     /locus_tag="SDD03_002855"
     CDS             316..570
                     /locus_tag="SDD03_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4305251.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="SDD03_002856"
     CDS             571..888
                     /locus_tag="SDD03_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4305252.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1522..>1676)
                     /locus_tag="SDD03_002857"
     CDS             complement(1522..>1676)
                     /locus_tag="SDD03_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG4305253.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT          588 a          263 c          317 g          508 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc
     1621 tagcccttta acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaag
//
LOCUS       DARDHU010000201         1676 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000201 DARDHU010000000
VERSION     DARDHU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.207"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            75..1622
                     /locus_tag="SDD03_002858"
     CDS             75..1622
                     /locus_tag="SDD03_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG4305254.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          561 a          306 c          345 g          464 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaagcag
      361 acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg gaagaacaat
      421 tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta cgagaagaaa
      481 ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc tattgttgta
      541 aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct aaacctttga
      601 tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg aaatttggtc
      661 agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg ttggatattt
      721 cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta gaccctttag
      781 gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat gaaacaagtt
      841 atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt acaggaaaag
      901 acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga gatgttccga
      961 aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc gggtataatg
     1021 ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag
     1081 caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc aaacaattgg
     1141 ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag cgattaagct
     1201 gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg gcaacgatag
     1261 atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg
     1321 gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt gcggagcgaa
     1381 atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt cttgaaggag
     1441 cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac ggattgaatc
     1501 ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta ctttcagcag
     1561 aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt tctcgtaact
     1621 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg tacagg
//
LOCUS       DARDHU010000202         1664 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000202 DARDHU010000000
VERSION     DARDHU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.208"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SDD03_002859"
     CDS             131..505
                     /locus_tag="SDD03_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4305255.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SDD03_002860"
     CDS             complement(619..1248)
                     /locus_tag="SDD03_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4305256.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="SDD03_002861"
     tRNA            complement(1382..1454)
                     /locus_tag="SDD03_002861"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DARDHU010000203         1651 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000203 DARDHU010000000
VERSION     DARDHU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.209"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            141..1607
                     /locus_tag="SDD03_002862"
     CDS             141..1607
                     /locus_tag="SDD03_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG4305257.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          337 g          556 t
ORIGIN      
        1 ttttttttat gatattgcca ttaaaaatta cttgctttta tagaaaatta gtttatattt
       61 cttaaataag ttttttagtt aaatttttcg gtaaagatgt ataatatttt ctgaaaattt
      121 aaagaattgg ggtatagatt atggaaaaac agcaagacaa gggcttcttc gggcagccga
      181 agggattgtc aactttgttc ttcactgaga tgtgggaacg atttagttat tacggtatgc
      241 gtgcattact tatctattat atgtacgata cagttgccaa cgggggactt ggattaccac
      301 gagcaacagc attagctatc atgtcgatct acggatcttt gatctacatg tctagtatcg
      361 tcggcggatg ggtatctgac cgagtgttag gagcaaaacg cactgtattc ttcggtggag
      421 ttttcattat gcttggccac atcgtcttgg ctttaccatt tggtgtaagt acattgtttg
      481 tttcgatggc attgattatt atcggaaccg cctttttgaa accaaatgtt tctggcatgg
      541 tcggacatct ttattcaaag actgacttgc gaagagatgc aggattttcg attttttaca
      601 tgggtgtcaa cttaggggca ttattagcac cactaatcgt tggtacaatc ggtcaagaat
      661 ataactatca tttagggttc tctttagctg ctattgggat gttctttgga ttattacaat
      721 acatcatcca aggacgcaag acattagacg gagtgggtac aacaccgccg aatcctttga
      781 caactgaaga acgtaaagga ttcatccgta acttgatcat cgcacttgtt gttatcgctg
      841 cagtattcgg cggggcacaa ctaactggtc atctgacaat tgactttttt gtcaatacaa
      901 tcagtgtatt ggggattatc ttaccgttgt attactttat caaaatgctg acttcaaaag
      961 atgtaactgc tgaagaaaaa ccaagagtat gggcttatat cccattattc cttgcagcaa
     1021 tcattttctg gtcattacaa gaacaaggtt catctatctt agctttattt gctagtgaac
     1081 gtacacaatc tacgctattt ggtgtaacga tccaagcaag ctggtatcaa tcattgaatc
     1141 caattttcat ctttattctg acaccagtat tcgtaacgat gtggacgaaa cttggtgaac
     1201 gtcaaccatc tacagtagtg aaattctctc ttggactttt atttgcagga ttatcgtact
     1261 tgctattgat gttgccgggt atgctttatg gtacaagtgg aaaagtaagt ccattgtggt
     1321 tgttgggaag tttcttcatc gtggttattg ctgaattgtg tctttcacca gttggtttgt
     1381 ctgtgacaac aaaattagca ccacgagcat ttgaatcaca aacaatcgct atttggttct
     1441 tagctgatgc ttcttcacaa gcaatcaatg cacaaattgc acgcttctac acaccgggaa
     1501 cagaatctgc ttacttcggt attatcggtg cagtagcaat cgtcggtggt atcttgctat
     1561 ttactgtgaa agaaccaatc aaaaaactaa tgggctctat tcattaatca atgaacataa
     1621 aaacaggaat tgcttatttg cagcagttcc t
//
LOCUS       DARDHU010000204         1563 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000204 DARDHU010000000
VERSION     DARDHU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.210"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          590 a          179 c          316 g          478 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caa
//
LOCUS       DARDHU010000205         1547 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000205 DARDHU010000000
VERSION     DARDHU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.211"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..340
                     /locus_tag="SDD03_002863"
     CDS             128..340
                     /locus_tag="SDD03_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305258.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            502..1020
                     /locus_tag="SDD03_002864"
     CDS             502..1020
                     /locus_tag="SDD03_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG4305259.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1027..1539
                     /locus_tag="SDD03_002865"
     CDS             1027..1539
                     /locus_tag="SDD03_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305260.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          607 a          181 c          241 g          518 t
ORIGIN      
        1 cactatatat tccgaaaagt gagtatctcc attcataaaa taatcaacat atttaaaaaa
       61 tctaattagc ttttttagtc aatctaaagt tctttattct atgatataat gttactgtag
      121 aaagtttgtg gcggtttggc aaatccaatt gaaggagcgt ggtgcctatg ggcttattag
      181 cttatcggac gtactctgta gaaaggaaat gcctgatttg ttctcattgg atcaaatcat
      241 tgctattatc ggagcagcag cagtagttat tactgccgta gcgaaagcta gcgattcgat
      301 tttagcttgg ttaaagttca aaaaagaaca aaaaaaatag agccgtctca acttttggcg
      361 aggtagacga gctctaaagc tttatttaca tgccatccgc tttgaacgga ctacactggg
      421 catgtgacag catgcccttt catttatatt atatctcatt cttttatata atgaaagtaa
      481 ataaatcatg gaggataaaa tatggataaa tttaatagat taacattaat aaatcagttt
      541 gaaattttaa aagctttaaa tcctaacgaa gaaaaatact atgctgaaaa aatagaaata
      601 ctaacagaag gttacgaata tcactatagt gaaatatttg aaaatatttc tgatccattg
      661 ccagaagaag attcgcggtt tgttctagac atattaaata tgtatagaga tattactttt
      721 agtaaaaata aattaaaaga tataaattca attgataatt attatataca atttaaaggt
      781 tttgacttta atagctctac cgaatttaag ttagcacttt atgcacaatt ttttattgaa
      841 aaattaaata ggtttcaaga aatagttgaa gatagtgatt ttaacacttt taatagccat
      901 aaaccgatga gaggtactta tataaaattt ctcgaaaatt ataatgattt aaaatctacc
      961 aataattatc aatttggaaa cttgtcttat gagatgataa gtaaagttct aagtttgtga
     1021 tgataaatga aattaaatag tataaatgat ttatttgcta ttataggtgt tattgctaac
     1081 atactttttt cgtgtttgaa ttttagattt actcaaaaga acaaaaagaa caaactcagt
     1141 atagaacaaa tgaaaaaaga cattgaaact gatttaaaaa atgaccataa agaaagaaga
     1201 aaaattgaag aatatacttc tttaatggaa aagtgtaaag caactgatgc aatttttagc
     1261 tttttaagaa aaagtgatga gataatcaca cgtgaaaata tagatttaga aaatgagatt
     1321 agtaaacttg aatctatctt ttccgatata aattctctta agtatttaat cgtcgaagaa
     1381 aattcggata gctttttaga atttgaaaaa tatctaacag atcttataga taatagaaat
     1441 aatgaattaa tgagacaaaa cattgcaaga ttccttacta aattagtttc tgaatggaaa
     1501 agtattattt ctcaaaatat ggataaatta agatattaag gatgtta
//
LOCUS       DARDHU010000206         1502 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000206 DARDHU010000000
VERSION     DARDHU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.213"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..476
                     /locus_tag="SDD03_002866"
     CDS             138..476
                     /locus_tag="SDD03_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305261.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(681..1256)
                     /locus_tag="SDD03_002867"
     CDS             complement(681..1256)
                     /locus_tag="SDD03_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG4305262.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          464 a          312 c          256 g          470 t
ORIGIN      
        1 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
       61 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
      121 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
      181 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
      241 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
      301 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
      361 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
      421 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
      481 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
      541 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt
      601 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
      661 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
      721 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
      781 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
      841 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
      901 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcata aaagccactc
      961 attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact
     1021 gtctctgcac gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc
     1081 atgatttccg gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac
     1141 ggaaaaactt ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct
     1201 cgataataat tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta
     1261 atccctccca atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg
     1321 ctttcaattt ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt
     1381 attctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt
     1441 tttgaaattg ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg
     1501 gt
//
LOCUS       DARDHU010000207         1474 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000207 DARDHU010000000
VERSION     DARDHU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.214"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            286..693
                     /locus_tag="SDD03_002868"
     CDS             286..693
                     /locus_tag="SDD03_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305263.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="SDD03_002869"
     CDS             707..1108
                     /locus_tag="SDD03_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305264.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..>1474
                     /locus_tag="SDD03_002870"
     CDS             1110..>1474
                     /locus_tag="SDD03_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305265.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKL"
BASE COUNT          566 a          180 c          258 g          470 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aact
//
LOCUS       DARDHU010000208         1468 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000208 DARDHU010000000
VERSION     DARDHU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.215"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(943..1356)
                     /locus_tag="SDD03_002871"
     CDS             complement(943..1356)
                     /locus_tag="SDD03_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG4305266.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          538 a          241 c          216 g          473 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatac
//
LOCUS       DARDHU010000209         1453 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000209 DARDHU010000000
VERSION     DARDHU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.216"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SDD03_002872"
     CDS             complement(<1..625)
                     /locus_tag="SDD03_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG4305267.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SDD03_002873"
     CDS             complement(784..1050)
                     /locus_tag="SDD03_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4305268.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SDD03_002874"
     CDS             complement(1040..1396)
                     /locus_tag="SDD03_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4305269.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DARDHU010000210         1440 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000210 DARDHU010000000
VERSION     DARDHU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.217"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SDD03_002875"
     CDS             105..1406
                     /locus_tag="SDD03_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4305270.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARDHU010000211         1424 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000211 DARDHU010000000
VERSION     DARDHU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.218"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..276)
                     /locus_tag="SDD03_002876"
     CDS             complement(<1..276)
                     /locus_tag="SDD03_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG4305271.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSI"
     gene            401..1186
                     /locus_tag="SDD03_002877"
     CDS             401..1186
                     /locus_tag="SDD03_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG4305272.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1179..1421
                     /locus_tag="SDD03_002878"
     CDS             1179..1421
                     /locus_tag="SDD03_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305273.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          533 a          232 c          218 g          441 t
ORIGIN      
        1 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
      361 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
      421 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
      481 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
      541 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
      601 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
      661 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
      721 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
      781 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
      841 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
      901 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
      961 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     1021 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     1081 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     1141 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     1201 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     1261 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     1321 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     1381 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DARDHU010000212         1418 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000212 DARDHU010000000
VERSION     DARDHU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.219"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SDD03_002879"
     CDS             complement(150..1337)
                     /locus_tag="SDD03_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG4305274.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DARDHU010000213         1355 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000213 DARDHU010000000
VERSION     DARDHU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.221"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SDD03_002880"
     rRNA            <1..1355
                     /locus_tag="SDD03_002880"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARDHU010000214         1348 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000214 DARDHU010000000
VERSION     DARDHU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.223"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            82..>1348
                     /locus_tag="SDD03_002881"
     CDS             82..>1348
                     /locus_tag="SDD03_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG4305275.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIF"
BASE COUNT          458 a          260 c          256 g          374 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttt
//
LOCUS       DARDHU010000215         1344 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000215 DARDHU010000000
VERSION     DARDHU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.224"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            14..>1344
                     /locus_tag="SDD03_002882"
     CDS             14..>1344
                     /locus_tag="SDD03_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305276.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVI"
BASE COUNT          453 a          173 c          183 g          535 t
ORIGIN      
        1 aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt
       61 tatagtttat gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat
      121 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
      181 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      241 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      301 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      361 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      421 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      481 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      541 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      601 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      661 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      721 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      781 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      841 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      901 taactttaaa aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat
      961 tctactttgt acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat
     1021 tcaagctcct tggcgtttta tgatattttc aacattgttc atatcttttt cagtatctct
     1081 atttatttct ccacagttgg aaaaattttc ttatacaagg agaagaaatt ttatactatt
     1141 attaatatta gctatttcaa ttttaaatta ttcatcagta gttaacatgg tgaaagaaat
     1201 gaattttcaa ggaacttatt ctaatgaaca aatggaaaat aaacttgaag aatttgagaa
     1261 ttttgattat gtacctatag gctataaaaa agatcaattg cttttgaaaa accatcaagt
     1321 tacagaagat ggtcaagtta ttta
//
LOCUS       DARDHU010000216         1339 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000216 DARDHU010000000
VERSION     DARDHU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.225"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1309
                     /locus_tag="SDD03_002883"
     CDS             <1..1309
                     /locus_tag="SDD03_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEG4305277.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          569 a          102 c          226 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaa
//
LOCUS       DARDHU010000217         1336 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000217 DARDHU010000000
VERSION     DARDHU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.226"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..221
                     /locus_tag="SDD03_002884"
     CDS             <1..221
                     /locus_tag="SDD03_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG4305278.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="SDD03_002885"
     CDS             218..1237
                     /locus_tag="SDD03_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG4305279.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT          449 a          244 c          275 g          368 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat caccca
//
LOCUS       DARDHU010000218         1276 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000218 DARDHU010000000
VERSION     DARDHU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.227"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            43..453
                     /gene="spx"
                     /locus_tag="SDD03_002886"
     CDS             43..453
                     /gene="spx"
                     /locus_tag="SDD03_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG4305280.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            467..823
                     /locus_tag="SDD03_002887"
     CDS             467..823
                     /locus_tag="SDD03_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG4305281.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          468 a          182 c          238 g          388 t
ORIGIN      
        1 aattactgta gaattggatt ataattaaaa atgagtgaga aaatgataaa agtatatact
       61 tcgcctagct gtacttcctg tagaaaagca agagcatggt tgcaagcgaa tcaacttgaa
      121 tttgaagaga agaatatttt tgccgaaccg ttaacagaaa gtgagatcaa aaagatcttg
      181 gcagcgactg aaggaggagt tcaagatatt atttccactc ggtcgaaaat ttacgaaaag
      241 ctgggcatag attttaacga attaacttta aagcaaatga tcgtcttatt caaagaatat
      301 ccttcattat tgagacgacc tattttgatt gatgaaaaaa gaatactaat cggatttaat
      361 gaagatgaga tccggtcatt tgtccctcgt gaaattcgat gtgttgccag aaatctgatc
      421 tatttacaaa acaataacat cttaaattca tagggaggga gccttcgtgg aagtgaaagt
      481 ctatggatca gcgcactgtg taacaagtct gaaggcgaaa caatggttag aacaccatca
      541 attgccttat caatttgttg acttagaaga acgcgatcta agtacagaag aagcagaaga
      601 attatgcagt tttgaagaag tgcaagcaga acagctattt gcgacttggt ctgaggcttt
      661 tcacaacatt gcgattgacc tttcctgtgt aggaaaaaaa caactgattt ttttatgcac
      721 acaacatacc aatctcctca gacgtccttt gattattatc gatgaagttc tatttattgg
      781 atacaatgag cagttgatgg aacagcttat cttaaataaa tagactttaa ctgttttaat
      841 caagtcactg agcatttcat acagttgtaa cagtcgaaca cttagcatca agcgtgttat
      901 gtgttctttt tttgacttta tttttctact atagagacat gctcaattta tgtggtcaaa
      961 attcaaatct aggtggggat atagagttaa aaatatattg acatgtgtaa atttacatga
     1021 tatattcatt tctgttgttt taagtagcaa agcaataaaa tagtatttta aaaaacatat
     1081 aaaatagttg ttgacatgtt agaaatgaca tgataagata actgagttgt taaaaaagaa
     1141 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
     1201 acgataagtt atgataaatg agttgctgaa agatttcgaa aaacgaaatc gaaaaaaact
     1261 tttaaaaaag agttga
//
LOCUS       DARDHU010000219         1272 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000219 DARDHU010000000
VERSION     DARDHU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.228"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            265..957
                     /locus_tag="SDD03_002888"
     CDS             265..957
                     /locus_tag="SDD03_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4305282.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          554 a          144 c          183 g          391 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc tt
//
LOCUS       DARDHU010000220         1267 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000220 DARDHU010000000
VERSION     DARDHU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.229"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            139..1011
                     /locus_tag="SDD03_002889"
     CDS             139..1011
                     /locus_tag="SDD03_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4305283.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          394 a          230 c          286 g          357 t
ORIGIN      
        1 ttttaaaaaa tgataaaaat tcaaaaagaa aaaatagtag ttatttactt gtcatgttac
       61 taaataaaaa aataatttaa atatgatata tagtcattta tttagtgatt tcattctaaa
      121 ataaagggag aaatgtctat gctatacggt gcaattgaag caggcggcac aaaatttgtg
      181 tgtgccattg gcgatgaaga aatgacgatc aaggaaagag tgagctttcc gacaacgaca
      241 cctgaggaaa caatgccttt agtcattgac ttcttcaaac aataccaagc agatttagca
      301 gggattggga ttggctcatt tgggcctatc gatatccatc gcgattccgc cacttatggg
      361 tacattacct cgaccccgaa attggcatgg caaaattttg atttcatcgg taccatgaaa
      421 aaagaatttc cgatcccgat ttcttggaca acagatgtca atgcggctgc ctatggtgag
      481 tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt actatacgat cggtacgggt
      541 gttggcggcg gtgccttaca agaagggcgc tttatcgaag ggtttagtca tccagaaatg
      601 gggcatatgc tcgtcgtccc tcatccaaaa gatgactttg ctggcagctg tccatttcat
      661 ggcaactgtt tagaagggat ggccgctgga cccgccatcg aaaaacgctt agggaaaaaa
      721 ggacaagagg ttgcagaaga tgatccttat tgggaaatcg aagcctccta tattgctcaa
      781 tgtgcctaca atacaacctt gatgttttct ccagatgtga tcatttttgg cggtggtgtg
      841 atgaaacaac gccatatgct gcaaaacgtc cacgacgcat ttgcaaaatt ggtcaatggg
      901 tatgtcgaaa cacctgagtt aagcaaatat atcgtgaccc cagcattaga agacaacgct
      961 ggaacgctcg gttgtttggc attggctcga gaagcggtct tacattcata aacgaaaaaa
     1021 ggctcttcga catttagtgt tggtagagaa agatacaact gaaaatttag tcataaccag
     1081 ttaggcagag tgcttaactg gtttttgttt tacttggaag atcaagcctt gaatgagata
     1141 aattcttgct cgaaagttat ggaaatttcg atagccgtag gccacttgta ttgtgcaata
     1201 gttttttaca cgccgtttgt ataattaagc gagacaaata aaaaagccat ttatgttaca
     1261 ctcaaat
//
LOCUS       DARDHU010000221         1263 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000221 DARDHU010000000
VERSION     DARDHU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.230"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..1097
                     /locus_tag="SDD03_002890"
     CDS             18..1097
                     /locus_tag="SDD03_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4305284.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          375 a          301 c          310 g          277 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaccc atgataacac agtttcacgc aagggaaaac
       61 atctttccta ctcagaacgt tgccaaatcg ctatcctgaa aaaggaaggc tattccaacc
      121 gtcaggttgc caaagtgctg ggacgtgttc cgcaaaccat aaacaatgag attgatcgcg
      181 gaaccatcac ccagttaaaa cgccaaacgc aaaatggaaa agtgtatgac acctactatt
      241 ctgtttatga cgctgatgca ggacaggcct tctatgaaaa gcaacgactg aactgtggca
      301 gacgccccaa atgggcgaac acaaatgcct tcattgattg ggcagatgac aaaatgttaa
      361 atgaaaaatg gtcgccggac gtcgtggtcg gatttgccag aaaacacgag ctgttcgatt
      421 gtgccatcat tcctggcaca accgctcttt atcaatggat tgaccgcgga atcatgcgaa
      481 caaagaacat ggacttgctt gaaaaactgt cccgtaaaat caaagcggtc cgcccaggac
      541 accgtcccaa caagcgtgtt ctcgggacgt caattgaaga acgccctaaa gaagttgaaa
      601 cacgggaaac gttcggtcat tgggaaatcg acacggtagt gggaaacaag accaagtcgg
      661 atgccgtgct gctgacgctg gctgaacggc agacacgctt tgaagtcatc ctaaagttga
      721 acgggaagga caccgaatcg gtggaccaag cggttcaaca gctaagagaa cgggctggcg
      781 ctaacttcga tcaattattt aagaccgtta catccgacaa tggctcggag ttttctgggc
      841 tacacgatgc tctgcaagat gtagctgacg tctatttcag ccacccctat gcttcttggg
      901 agcgtgggac gagcgaaaac cagcacaaat taattcgccg tttcgtcccc aagggaagcc
      961 cgattagtga ggtgagcgag aaccagattt tgcggatcca gcgttggatg aacgattacc
     1021 cgcggaaaat tttgggttat gcgacaccac atgatacatt cgtccaggca ttcaagcaag
     1081 aacggttggt ggcttgaccc attattcctt gataacgatc ctcactcggg acatggcttc
     1141 atgtctggaa agctggcctg gggccagaat cgtctgaaga ctatcatacc tcagttcatt
     1201 atcaaggcct cactgattta gaataacgag gcagttacgg tatcaaacaa atgagttacc
     1261 tgt
//
LOCUS       DARDHU010000222         1259 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000222 DARDHU010000000
VERSION     DARDHU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.231"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..1097
                     /locus_tag="SDD03_002891"
     CDS             18..1097
                     /locus_tag="SDD03_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4305285.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          332 a          342 c          356 g          229 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaacc atgataacac agtttcacgc aagggaaaac
       61 acctaaccta ctcagaacgt tgccaaatcg ccatcctgaa aaaggaaaac tattccaacc
      121 gtcaggttgc ggatgtcctg ggctgcgtgc cgcagaccat aaacaacgaa atcaaacgcg
      181 ggaccgttac tcagcttaac cgccagaatc agaacgggaa ggtctacgac tattatcatg
      241 acgtctacga cgccgatgcc gggcaggcgg cctacgagaa gaaccgcttg aactgcggca
      301 gacggccctt atgggccgac acggacgcct tcattgaatg ggcggacgac atgatgctgt
      361 tggaaaaatg gtccccggac gtggtggtgg gctttgccag aaaacacgaa ttgttcgacc
      421 cggccattat ccctgggacc accaccctct atcagtggat cgaccgtggc atcatgaaaa
      481 cgaagaatat ggacctgcta gaaaaactgt cccgcaaacc gaaatcgtcc cgtcatggca
      541 accgcccgaa taaacgggtg ctagggaaat ccattgaaga gcgcccggag gcggttggta
      601 cccggaaaac cttcggccat tgggaagtcg acacggtcgt aggaaacaag accaagtcgg
      661 atgccgtgct gctgacgttg gtggaacggc agacacgctt cgaggtcatt ctgaagctgg
      721 ccgggaagga tgccgaatcg gtcaaccaag cggtccgaca gctgagagaa cgggctggtg
      781 cgtacttcgg ccagctgttc aagacggtta cctcggacaa cggctcggag ttcgcgggac
      841 tgcacgatgc cttgcaggat gtggcaaaca tctacttcag ccacccctat gcttcctggg
      901 agcgcgggac gagcgaaaac caacacaagc tgattcgccg tttcatcccc aagggcagcc
      961 caatcggcaa cgtgagcgaa aaccaaatct tgcggattca gcgttggatg aacgattacc
     1021 cgcgaaagat tttgggctat gcaacgccgc atgacacgtt cgtccaggca tttaggcagg
     1081 aacggttgac ggcttgacac gtgggccctt gacaacgagc ctccctcggc atggcttgtg
     1141 tctggaaagc tggccggggc cagccagtcg ggagactacc atgcctcagc tcattgtcaa
     1201 gggcccactc gattagaaaa attagacggt tggtggtaac gagcaaccgg gtcaccatg
//
LOCUS       DARDHU010000223         1246 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000223 DARDHU010000000
VERSION     DARDHU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.232"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            200..742
                     /locus_tag="SDD03_002892"
     CDS             200..742
                     /locus_tag="SDD03_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305286.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="SDD03_002893"
     CDS             898..>1246
                     /locus_tag="SDD03_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG4305287.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DARDHU010000224         1215 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000224 DARDHU010000000
VERSION     DARDHU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.233"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            414..947
                     /locus_tag="SDD03_002894"
     CDS             414..947
                     /locus_tag="SDD03_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG4305288.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DARDHU010000225         1193 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000225 DARDHU010000000
VERSION     DARDHU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.234"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1076
                     /locus_tag="SDD03_002895"
     CDS             <1..1076
                     /locus_tag="SDD03_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG4305289.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DARDHU010000226         1143 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000226 DARDHU010000000
VERSION     DARDHU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.235"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="SDD03_002896"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="SDD03_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG4305290.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DARDHU010000227         1105 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000227 DARDHU010000000
VERSION     DARDHU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.236"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SDD03_002897"
     CDS             <1..1087
                     /locus_tag="SDD03_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4305291.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DARDHU010000228         1103 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000228 DARDHU010000000
VERSION     DARDHU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.237"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..634)
                     /locus_tag="SDD03_002898"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="SDD03_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          341 a          190 c          189 g          383 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tat
//
LOCUS       DARDHU010000229         1069 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000229 DARDHU010000000
VERSION     DARDHU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.239"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..969
                     /locus_tag="SDD03_002899"
     CDS             <1..969
                     /locus_tag="SDD03_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4305292.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DARDHU010000230         1061 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000230 DARDHU010000000
VERSION     DARDHU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.240"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(36..992)
                     /locus_tag="SDD03_002900"
     CDS             complement(36..992)
                     /locus_tag="SDD03_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305293.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DARDHU010000231         1051 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000231 DARDHU010000000
VERSION     DARDHU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.241"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="SDD03_002901"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="SDD03_002901"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG4305294.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DARDHU010000232          996 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000232 DARDHU010000000
VERSION     DARDHU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.242"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..912)
                     /locus_tag="SDD03_002902"
     CDS             complement(25..912)
                     /locus_tag="SDD03_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG4305295.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          322 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaatt
//
LOCUS       DARDHU010000233          994 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000233 DARDHU010000000
VERSION     DARDHU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.243"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="SDD03_002903"
     rRNA            complement(<1..>994)
                     /locus_tag="SDD03_002903"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DARDHU010000234          994 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000234 DARDHU010000000
VERSION     DARDHU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.244"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..555
                     /locus_tag="SDD03_002904"
     CDS             <1..555
                     /locus_tag="SDD03_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305296.1"
                     /translation="DLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRN
                     ISHKVRVNNRKEVCDDFKMVYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLL
                     TFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFL
                     GRSHKGFQQAEGELEQMLSQPMEN"
BASE COUNT          324 a          176 c          188 g          306 t
ORIGIN      
        1 gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg
       61 atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac
      121 gtttttcgta atatcagtca caaagtgcgt gtcaataatc gtaaggaagt ctgtgatgat
      181 tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tagaagcacg tggtgctttt
      241 gcgaaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac
      301 ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg
      361 attgaatcct ttaataagca aatcaagaaa tacagccacc gcaaggaaca gttccaaaac
      421 gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta
      481 ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctgagtcaa
      541 ccgatggaga attagaaaca atctacagta gggaagaacc atttacacaa aattattgac
      601 gctcccaagc taaaactcct attaatcgat ctactaaaaa taaataaaaa aattcttttc
      661 aatttttatt tatggtatga cataaataaa gtaaagttat tcttctaggt taatcttatt
      721 aatactcttt ctcactagtg ttgcatgcaa cactagtgag aaagagtatt aaatatactg
      781 caaggcaaga catttggtaa ttgaagaata acaagcaaaa aaatagcagt caggtctctt
      841 gacaaagtat agttgctatt ctttttttag gccctacttt aatgaaattt ttgtttggag
      901 ggaacgactt cattcctttg attatttcag ttgaatttaa tagagaagcc tggcgtctat
      961 taagaaatac ttttcttgat gagcgtcaat tttt
//
LOCUS       DARDHU010000235          986 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000235 DARDHU010000000
VERSION     DARDHU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.245"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SDD03_002905"
     CDS             <1..704
                     /locus_tag="SDD03_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4305297.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DARDHU010000236          931 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000236 DARDHU010000000
VERSION     DARDHU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.246"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>931
                     /locus_tag="SDD03_002906"
     CDS             <1..>931
                     /locus_tag="SDD03_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4305298.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DARDHU010000237          905 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000237 DARDHU010000000
VERSION     DARDHU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.247"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..>905
                     /locus_tag="SDD03_002907"
     CDS             33..>905
                     /locus_tag="SDD03_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4305299.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          313 a          157 c          170 g          265 t
ORIGIN      
        1 tccgaccaaa gaattataag gagtgagtac aaatgaccta tacccatctt acaacggatg
       61 aacttgtaat aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt
      121 gcttgaatcg ttcaagacaa acgatccata aagtatatct gttcttcaag caaggaaaat
      181 cagccttaga atattatcaa cagtataaga aaaacaaatc aaactgtggt agacgtccgc
      241 ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa ggatggacac
      301 ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt accatttatc
      361 gtatgttcaa aaaggggcta tttgattctt ctgacttacc gatgaaaggc aagcgtaaac
      421 cgaatggaca tcaagaaaga cgtggaaaac aaactttccg ccgctctatt catgaacgtg
      481 aaaaggatta tagccaattc tcaaatgagt ttggtcacct tgaaggtgac actatcgtag
      541 gtctgaaaca taaaagtgct gtaattacct tagttgaacg attatcaaaa gttatcatca
      601 cattgaaacc gtgtggtaga caagcgattg atattgaaaa aaaattaaat caatggtttg
      661 aatctgtacc gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag gaattttcaa
      721 attggaaaca gatcagtaat gtcaatgata ttgccattta tttcgctgat ccaggaacgc
      781 cgtctcaaag aggcctaaac gagaattcta acggattgtt acgtagagat ggtttattga
      841 aatctatgga tttcaattca gtagatgaat tttttattca atctgtcgca tctaaacgaa
      901 ataat
//
LOCUS       DARDHU010000238          905 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000238 DARDHU010000000
VERSION     DARDHU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.248"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            266..889
                     /locus_tag="SDD03_002908"
     CDS             266..889
                     /locus_tag="SDD03_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4305300.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          276 t
ORIGIN      
        1 ttagttttaa aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa
       61 tacttaaagg aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt
      121 atttgttgtt tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg
      181 atttttcagc ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa
      241 atagattgga taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga
      301 tcaaagttta gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc
      361 tgaagacgat ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc
      421 agagctgcaa gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct
      481 tgatcgatta gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa
      541 aggtgcttct attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt
      601 aagagacttg ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg
      661 gaaaaaaatc aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta
      721 tactggtaaa aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga
      781 tgaaattgtt gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg
      841 agttacaaga aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt
      901 tagaa
//
LOCUS       DARDHU010000239          890 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000239 DARDHU010000000
VERSION     DARDHU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.250"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(77..149)
                     /locus_tag="SDD03_002909"
     tRNA            complement(77..149)
                     /locus_tag="SDD03_002909"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:His,seq:gtg)
     gene            complement(152..225)
                     /locus_tag="SDD03_002910"
     tRNA            complement(152..225)
                     /locus_tag="SDD03_002910"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Trp,seq:cca)
     gene            complement(232..312)
                     /locus_tag="SDD03_002911"
     tRNA            complement(232..312)
                     /locus_tag="SDD03_002911"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(276..278),aa:Tyr,seq:gta)
     gene            complement(314..389)
                     /locus_tag="SDD03_002912"
     tRNA            complement(314..389)
                     /locus_tag="SDD03_002912"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Phe,seq:gaa)
     gene            complement(425..497)
                     /locus_tag="SDD03_002913"
     tRNA            complement(425..497)
                     /locus_tag="SDD03_002913"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(462..464),aa:Asp,seq:gtc)
     gene            complement(507..579)
                     /locus_tag="SDD03_002914"
     tRNA            complement(507..579)
                     /locus_tag="SDD03_002914"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(544..546),aa:Val,seq:tac)
     gene            complement(595..666)
                     /locus_tag="SDD03_002915"
     tRNA            complement(595..666)
                     /locus_tag="SDD03_002915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(631..633),aa:Glu,seq:ttc)
     gene            complement(681..770)
                     /locus_tag="SDD03_002916"
     tRNA            complement(681..770)
                     /locus_tag="SDD03_002916"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(734..736),aa:Ser,seq:gga)
     gene            complement(810..883)
                     /locus_tag="SDD03_002917"
     tRNA            complement(810..883)
                     /locus_tag="SDD03_002917"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Asn,seq:gtt)
BASE COUNT          249 a          244 c          204 g          193 t
ORIGIN      
        1 agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata
       61 ccccaagaat aaaaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc
      121 cggtgcgtta accacttcgc cacattcgcc atggcagggg cagcaggaat tgaacccaca
      181 ctaacggttt tggagaccgc tgttctacct ttaaactatg cccctataaa atggaggaga
      241 gtggattcga accactgaac cctaaggaac ggatttacag tccgtcgcgt ttagccactt
      301 cgctactcct ccatggtggc gcgggacaga atcgaactgc cgacacacgg agcttcaatc
      361 cgttgctcta ccaactgagc taccgcgcca tattttaaaa ttattaattg aataacaaga
      421 acgacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcatgttaac
      481 ccctacacca cggaaccagt tcattatgga ggttaacggg atcgaaccgc tgaccctctg
      541 cttgtaaggc agatgctctc ccagctgagc taaacctcca acattcaatt aaaatgaccc
      601 gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa
      661 cgggccaggt aaatttgcta cggagagtaa gggattcgaa cccttgagac agtttggcac
      721 catctacatg atttccaatc atgctccttc ggcctctcgg acaactctcc agagtgaaag
      781 aagctcaata ctctttctca aaatgagaac tccggcagta ggactcgaac ctacgacatc
      841 atgattaaca gtcatgcgct actaccaact gagctatgcc ggaataacat
//
LOCUS       DARDHU010000240          877 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000240 DARDHU010000000
VERSION     DARDHU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.251"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..877
                     /locus_tag="SDD03_002918"
                     /pseudo
     CDS             1..877
                     /locus_tag="SDD03_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          335 a          147 c          162 g          233 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
      661 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
      721 accaagctat tgatgaatta ttaacaactt ttgacatcaa tcgatcattg agtcataaag
      781 gctgtccttt tgataatgcc gtagctgaat caacttataa gtcgttgaaa gtagaatttg
      841 tctatcaata cacatttgaa accttacaac aattgga
//
LOCUS       DARDHU010000241          836 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000241 DARDHU010000000
VERSION     DARDHU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.252"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            17..>836
                     /locus_tag="SDD03_002919"
     CDS             17..>836
                     /locus_tag="SDD03_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG4305301.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          288 a          123 c          154 g          271 t
ORIGIN      
        1 aaaagaggac ttccatatga acgattttac tacagaaatt atggaaatac taatcaataa
       61 aggtgatttg gatgatttgt ttcgtcgtca cctagaattg gctattaacg ctttattaca
      121 agctgaatta acggtatttc ttgactatga aaagtatgat agagctggat ttaattcagg
      181 taattcccgt aatgggaatt attcgcgctc ttttaaaaca gagtacggtg aattgaattt
      241 agtaatacct agagatagga atggagactt ttctcaacaa actttacctg cttataaaag
      301 aaccaatgac tctttagaaa ccactattat tcagctagtt caaaaaggaa ttactatgtc
      361 cgaaatctct gatctaatcg aaaaaatgta tggtcattat tacaaaccac aaactatttc
      421 aaatatgagt aaaattgtgt ctgaagatgt ttcggcattt aaagaaagaa ctttagaagc
      481 taaatactca gtcattttta tggatgctac tcatattccc ttaaaaagac aaaccgtatc
      541 aaaagaagct gtctatatag taattggtat caggttggat ggaactaaag aggttctagg
      601 atttactata gctccaactg aatctgctta tgtttggaaa gagattcttc aagatctaaa
      661 agaccgtggt ttagaagagg ttttgttagt cgtaacagat ggtttaagtg gtattgacga
      721 tagtattcat agtgtttatc caaatgctca atttcaacaa tgttgtgtcc atatatctag
      781 aaatatcgct cataaggttc gtgttagtga tcgacaagaa atctgtagcg atttca
//
LOCUS       DARDHU010000242          818 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000242 DARDHU010000000
VERSION     DARDHU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.253"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            136..309
                     /locus_tag="SDD03_002920"
     CDS             136..309
                     /locus_tag="SDD03_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305302.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          275 a          131 c          103 g          309 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaat
//
LOCUS       DARDHU010000243          811 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000243 DARDHU010000000
VERSION     DARDHU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.254"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..672
                     /locus_tag="SDD03_002921"
     CDS             <1..672
                     /locus_tag="SDD03_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305303.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DARDHU010000244          768 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000244 DARDHU010000000
VERSION     DARDHU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.256"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..>768)
                     /locus_tag="SDD03_002922"
     CDS             complement(109..>768)
                     /locus_tag="SDD03_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG4305304.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          213 a          176 c          147 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcg
//
LOCUS       DARDHU010000245          753 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000245 DARDHU010000000
VERSION     DARDHU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.257"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..734
                     /locus_tag="SDD03_002923"
     CDS             <1..734
                     /locus_tag="SDD03_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG4305305.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          321 a           97 c          127 g          208 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagttcaac ttttggggtt cag
//
LOCUS       DARDHU010000246          745 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000246 DARDHU010000000
VERSION     DARDHU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.258"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>745
                     /locus_tag="SDD03_002924"
     CDS             <1..>745
                     /locus_tag="SDD03_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEG4305306.1"
                     /translation="YHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYT
                     DAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVTFTAGEGTSIESGT
                     TVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDA
                     DKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTY
                     PDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          300 a          121 c          169 g          155 t
ORIGIN      
        1 gctatcataa agtaaccttt actgcaggag aaggaacaag tattgaaagt ggaacaacag
       61 tctttgcagt gaaagacggc gtaagcttac cagaagataa acttccagtg ttgaaagcaa
      121 aagatggtta tacagatgcg aaatggccag aagaagcaac tcaaccaatt acagcagatg
      181 atacagaatt tgtatcaagt gcaacaaaac tggatgatat cattgaaaat ccaggagaaa
      241 acattcctgc aggctaccac aaagtaacct tcaccgcagg agaaggaaca agtattgaaa
      301 gtggaacaac agtctttgca gtgaaagacg gcgtaagctt accagaagat aaacttccag
      361 tgttgaaagc aaaagatggt tatacagatg cgaaatggcc agaggaagca actcaaccaa
      421 ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat aaatctgacg
      481 ctgacaaata taatcctgaa ggtcaaaaag tgactacaga attgaataag gaaccagacg
      541 catttgaggg aattaagaac aaagaagatt taccaaaaga tactaagtat acttggaaag
      601 aaaaagtaga tgttagcgca gctggaaata aaaaagggac tgttgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagatg ttacagtgac agacaatcgt tctgacgccg
      721 ataaatatga gccaacagta gaagg
//
LOCUS       DARDHU010000247          734 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000247 DARDHU010000000
VERSION     DARDHU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.259"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            53..733
                     /locus_tag="SDD03_002925"
                     /pseudo
     CDS             53..733
                     /locus_tag="SDD03_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          259 a          126 c          148 g          201 t
ORIGIN      
        1 aaaaaatcta ttttagtgta aaattgagaa aaaagacaga gaggacagag taatgaatca
       61 ttttaaaggc aaacaattca aaaaagacgt cattattgtc gctgttggtt actacctgcg
      121 ttacaatcta agctatcgtg aagttcagga attgttatat gatcgtggaa taaatgtttg
      181 tcatactacg atttatcgtt gggtataaga gtacagcaaa gtcctctatt atctttggaa
      241 gaagaaaaat agacaatcct tctattcatg gaaaatggac gaaacctata tcaaaattaa
      301 gggacgttgg cattatcttt atcgtgcaat tgatgcggac ggcttaacct tagatatctg
      361 gttacgaaag aaacgggata cgcaagcagc ctatgctttc ttaaaacgac tccataaaca
      421 gtttggtgag ccgaaagcaa ttgtgaccga taaagcacct tctcttggct ccgcctttag
      481 aaagttacag agtgtgggtt tatatactaa gacagagcac cgaactgtga agtatcttaa
      541 caatttaata gaacaagacc atcgacctat taaacgacga aataaatttt atcaaagtct
      601 ccgtacagcc tcttccacga ttaagggcat ggagaccatt cgaggaatat ataaaaagaa
      661 ccgaagaaat ggaacgctct tcggcttttc ggtgtctact gaaatcaagg tattaatggg
      721 aataacagcc taag
//
LOCUS       DARDHU010000248          733 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000248 DARDHU010000000
VERSION     DARDHU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.260"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SDD03_002926"
     rRNA            <1..>733
                     /locus_tag="SDD03_002926"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARDHU010000249          724 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000249 DARDHU010000000
VERSION     DARDHU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.261"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            478..>724
                     /locus_tag="SDD03_002927"
     CDS             478..>724
                     /locus_tag="SDD03_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305307.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPN"
BASE COUNT          286 a           85 c          129 g          224 t
ORIGIN      
        1 atcctaatac tgattttgtg tttaattgtt catgaagtga ataaacaatt gtgaattaaa
       61 aaaataaaaa cctagtataa tcactttaat aaattgttgg tagaaggtga tcaaaatggg
      121 agtaaacgaa atgatgaaag tcagattgag tttttaaatg ataccttcct cagaacttca
      181 aaacgaattc aaagattcgt tgacaaatct tgagcaagat catttaaaaa aatattttct
      241 cttcatttga cgaaaaatgt ttagaaatct tatataagtg aagataaagt aattggtgta
      301 attaattgta gagaatagcc tttgagcaat tgaagcgata caattattta gaaaatgaac
      361 ttcctccagc atactacatg attccttacg ggacgatgtt aaagaagaaa gcaagaaatt
      421 ttagaagtat tagtagataa actaatgggc ttttctcaat ttaagggagt agcccaaatg
      481 tttatcaaat atggtgcaga tacagtagca accatgttta cagaagaata tcaagtagac
      541 tattatgaag taatagataa atgtgtacat tttgtttatg aaaaagtgtg gaattttatg
      601 aattttgcga taagtgaaga aatcttacga gtacaagaaa acagaacagg aatatttacc
      661 agagacagaa agcttaattt gattgcgcat tatatacgga atcgctggtc atcaatacca
      721 aatt
//
LOCUS       DARDHU010000250          712 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000250 DARDHU010000000
VERSION     DARDHU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.262"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SDD03_002928"
     CDS             complement(<1..446)
                     /locus_tag="SDD03_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4305308.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARDHU010000251          694 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000251 DARDHU010000000
VERSION     DARDHU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.263"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..227
                     /locus_tag="SDD03_002929"
     CDS             <1..227
                     /locus_tag="SDD03_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEG4305309.1"
                     /translation="GKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVS
                     FIEMWLPETAIRMEIDQLNRQQRLFGDENE"
     gene            401..>694
                     /locus_tag="SDD03_002930"
     CDS             401..>694
                     /locus_tag="SDD03_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305310.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
BASE COUNT          265 a          116 c          137 g          176 t
ORIGIN      
        1 ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca aaattttggt
       61 ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt aagagattcg
      121 atgatgctgt ttcatttatt gaaatgtggt taccagaaac tgcgatccgc atggaaatcg
      181 atcaactgaa cagacaacaa agacttttcg gtgatgaaaa tgaatagagc tgaagcgcta
      241 agaataggga cggtaattgc taatcgctgg tggagacaca ataaaccaaa catcctaagc
      301 caacaacata tcgaaaagca aaaagcatgg caacaaataa aaaagtgact cagccggcaa
      361 gcatagagtc acaaaacata tctaaggaga attttagcat atgaataaag aactttccac
      421 tttagatcaa tatttgactg attctgaatg gggcaagtcg aatatcaagg aaacaaataa
      481 tcgaaaaatc agacgaaatc ttttgacgaa cgaagaacta tcatgtgatc aagatgactt
      541 gggaaatttt gtgagtattt gggatcatgt ttaccttatc catctatcaa aacattcaaa
      601 taaacctgaa tacatctatg tcatcgaaga tggcttgatt gatgcgctag aagagtacaa
      661 cagagataac ttgattgata tctcttatta cgga
//
LOCUS       DARDHU010000252          665 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000252 DARDHU010000000
VERSION     DARDHU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.264"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..124
                     /locus_tag="SDD03_002931"
     CDS             <1..124
                     /locus_tag="SDD03_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4305311.1"
                     /translation="TFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            447..563
                     /locus_tag="SDD03_002932"
     CDS             447..563
                     /locus_tag="SDD03_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEG4305312.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          276 a           73 c           86 g          230 t
ORIGIN      
        1 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
       61 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
      121 ttgacaaatc ataaataata aagaattgaa cttctgagaa aacaaagaat cttttttctt
      181 tttttgaatt gactcatgga ttggcgttaa aattttattt tgaaaaagtt atttctggtt
      241 taactcaaaa aaagagatat gaatctaata gtgatagaaa ggatgttatt tttcatgaaa
      301 tggttgtttt agatttaata catataacta atgaatgcta ttgaatgtat ttaaactaat
      361 taacaaaaga atttgaattg ttaataaaat ccaataaaat atattaataa agtagataga
      421 aataaaaaaa caaagactaa acatatataa ttggattaaa cgcaatatct gcaaaatatt
      481 gtaaatttaa agcctttgta gaaaaattta ctgaaagaca aactgataag attattcagt
      541 atcatattac ctatactgat taaaattttt tgaaacggga tactaaataa aaaatttatt
      601 tataacagaa ctaattattt aatgcggatg cgccaatact tggtcctgca agagctattt
      661 atttt
//
LOCUS       DARDHU010000253          645 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000253 DARDHU010000000
VERSION     DARDHU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.265"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            175..438
                     /locus_tag="SDD03_002933"
     CDS             175..438
                     /locus_tag="SDD03_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4305313.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            438..>645
                     /locus_tag="SDD03_002934"
     CDS             438..>645
                     /locus_tag="SDD03_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4305314.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVY"
BASE COUNT          248 a           80 c          129 g          188 t
ORIGIN      
        1 gatgaagtta taatcaaata tatgaagcct gtgataaaac ttgttaataa aaataaagga
       61 aacggataac actttacaat gctgttttgg tactccaatt taaacaaaac aatcgaattt
      121 acgtacttaa ttaagtacgt tagaatggag atataaatta gagggggagt agatatggca
      181 aaaacagtct taaatcctag ctcagcacgg aaaaattttt ataagttgtt gaaagatgta
      241 aatgagaatc atacggaaat tgaaatcatt agtgagcgca gtgaaaataa tgcggtgctt
      301 attggtttgg atgattggaa agcaatcaaa gaaacgctat tacttgagca aataggcact
      361 ttgggtgttg ttcgtaagcg agaaaaagat gatagtggat ttactaatgt tgatgaaatt
      421 gattgggata acttataatg aataactatt ctgtgatgat taaaaactcg gctaaagctg
      481 atttaaagaa aatcaaacag tctaatttga aacctcaatt tgagaaggtt attcagactt
      541 taaaggaaga tccctatctc cctacccaat catttgagaa gttaaaacct acacatgaag
      601 gtaggtattc tagaagattg aatagacaac accgggttgt ttata
//
LOCUS       DARDHU010000254          635 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000254 DARDHU010000000
VERSION     DARDHU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.266"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..270)
                     /locus_tag="SDD03_002935"
     CDS             complement(<1..270)
                     /locus_tag="SDD03_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305315.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="SDD03_002936"
     CDS             complement(276..>635)
                     /locus_tag="SDD03_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG4305316.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DARDHU010000255          602 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000255 DARDHU010000000
VERSION     DARDHU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.267"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            178..576
                     /gene="spxA"
                     /locus_tag="SDD03_002937"
     CDS             178..576
                     /gene="spxA"
                     /locus_tag="SDD03_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG4305317.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          210 a           93 c          118 g          181 t
ORIGIN      
        1 agcgagaatg ctggttttac tgtatattaa ccttagataa ctttcatttt tttaaaaaaa
       61 ttatgaaaaa aaggtagcta tttaaaagtt gattatgtat aatagaaaca gatgggagat
      121 tattatgatc ttctgttaca aaaaagtgat tcttatcacg aaaaggagtg gtgcacgttg
      181 ttaacattat atacttcccc aagttgtact tcctgcagaa aggctcgtgc gtggttgcaa
      241 gaacatcaga ttccatttgt tgaacgtaat attttttcag aaccattgaa cagttctgaa
      301 ttaaaagcga ttttacaaat gacagaagac ggaacagaag aaatcatctc gacacgttcc
      361 aaagtattcc aaaaactgaa catggattta gatgatctgc cgttacaaga attattggaa
      421 cttgtgcaaa acaatcctgg attgcttcgc cgtccaatca tgattgatga taaacgtctg
      481 caagttggtt ttaatgaaga tgaaattcgt cggttcctac cgcgcgatgt acgacaatta
      541 gaattacgtc aagcacaatt gatggctgga ttatagaagg ataatacgaa acttggaaaa
      601 ga
//
LOCUS       DARDHU010000256          588 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000256 DARDHU010000000
VERSION     DARDHU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.268"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..150)
                     /locus_tag="SDD03_002938"
     tRNA            complement(65..150)
                     /locus_tag="SDD03_002938"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="SDD03_002939"
     tRNA            complement(167..238)
                     /locus_tag="SDD03_002939"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="SDD03_002940"
     tRNA            complement(250..322)
                     /locus_tag="SDD03_002940"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="SDD03_002941"
     tRNA            complement(340..421)
                     /locus_tag="SDD03_002941"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="SDD03_002942"
     tRNA            complement(429..501)
                     /locus_tag="SDD03_002942"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="SDD03_002943"
     tRNA            complement(505..577)
                     /locus_tag="SDD03_002943"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DARDHU010000257          580 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000257 DARDHU010000000
VERSION     DARDHU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.269"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          190 a           89 c           90 g          211 t
ORIGIN      
        1 ttctgtgtta gaattttttt gaagagtatt atacaagcta aagcttttct tcattgccac
       61 tcaaatgagt ggctttttta tgtatccttt tatggattaa tgaaaggatg tttcacatag
      121 ttatacttct gtatatttga aaagttttac tttgattttt aaatagaaag acatttgggt
      181 taaattgtga gataataata aagaagagtt taaagcgcac cccaaaccac ttccccataa
      241 gtgtgttacg ctttaaactc ttttatattt gaagccatta aaaagcatac catatttttg
      301 aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt taattagtga cttatttttg
      361 attttatagc actgatacta taaaatatag atatcatcat attacacaat cttaatacta
      421 acttaaaaaa tatctccttt cacaagtatg gtgataaaat tcgttccggg ctaccttttt
      481 aggtagccta ctttaatctt tatacctttc tggatcaacg aaagtatact ttatatagtc
      541 ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc
//
LOCUS       DARDHU010000258          566 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000258 DARDHU010000000
VERSION     DARDHU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.271"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(146..445)
                     /locus_tag="SDD03_002944"
     CDS             complement(146..445)
                     /locus_tag="SDD03_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4305318.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          183 a          106 c           67 g          210 t
ORIGIN      
        1 taattaaagg ataaaataca atataaagaa acatctgttt ctttatttag aataaaaaaa
       61 tacagactct gttcaaataa atgaacctag cctgcattat gagattgact atacttttta
      121 attcattgag atgaataact cgtttttata aatgcccaaa agtaatcggt gccgaagcca
      181 ttcctcgacc taattctttt tcaaattttc ctattgttga tatctccata taaaacggtg
      241 atactggttt agacaattct cccctgattg ctaacggacg taaactacgt tccaaattat
      301 ttatgacttt aggataatac tgaccttttt ctaagcctgt ctttgcgtct atcaaaagta
      361 tacgttcctt ttctttgatc tctggattta aaacgagatc atataatttt agtaccatgt
      421 tttctatttc ttcactttta gacatcacga tttcctccca aaaatatatt tcttcccact
      481 acattctata aacaaagtat acgctacatc tagatttttt taaatcatta ctggattgtt
      541 aaaacctttc aatacactgt tatggc
//
LOCUS       DARDHU010000259          546 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000259 DARDHU010000000
VERSION     DARDHU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.272"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..516
                     /locus_tag="SDD03_002945"
     CDS             <1..516
                     /locus_tag="SDD03_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEG4305319.1"
                     /translation="KLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLT
                     LDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHR
                     TIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVC
                     TEIKVLLGIPA"
BASE COUNT          197 a           97 c          104 g          148 t
ORIGIN      
        1 aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg
       61 gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca
      121 gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct
      181 tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc
      241 ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa
      301 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      361 cgcaataaat tctatcgaag tttacgcact gcctctacca cgattaaagg catggaagcc
      421 attcgaggat tatataagaa aacccgaaaa gaaggcactc tcttcgggtt ttcggtctgt
      481 actgaaatca aggtattatt gggaatccca gcttaaatca tagataccgt aagggatttt
      541 attctt
//
LOCUS       DARDHU010000260          526 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000260 DARDHU010000000
VERSION     DARDHU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.273"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SDD03_002946"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SDD03_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG4305320.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SDD03_002947"
     CDS             complement(349..>526)
                     /locus_tag="SDD03_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305321.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARDHU010000261          514 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000261 DARDHU010000000
VERSION     DARDHU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.275"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..>514)
                     /locus_tag="SDD03_002948"
     CDS             complement(107..>514)
                     /locus_tag="SDD03_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG4305322.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DARDHU010000262          514 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000262 DARDHU010000000
VERSION     DARDHU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.276"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..132)
                     /locus_tag="SDD03_002949"
     CDS             complement(<1..132)
                     /locus_tag="SDD03_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG4305323.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DARDHU010000263          513 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000263 DARDHU010000000
VERSION     DARDHU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.277"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          156 a          108 c           74 g          175 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aat
//
LOCUS       DARDHU010000264          507 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000264 DARDHU010000000
VERSION     DARDHU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.278"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          227 a           70 c           81 g          129 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactc
//
LOCUS       DARDHU010000265          485 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000265 DARDHU010000000
VERSION     DARDHU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.279"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SDD03_002950"
     CDS             complement(62..259)
                     /locus_tag="SDD03_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4305324.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARDHU010000266          462 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000266 DARDHU010000000
VERSION     DARDHU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.280"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..430)
                     /locus_tag="SDD03_002951"
     CDS             complement(<1..430)
                     /locus_tag="SDD03_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4305325.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DARDHU010000267          460 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000267 DARDHU010000000
VERSION     DARDHU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.281"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(20..91)
                     /locus_tag="SDD03_002952"
     tRNA            complement(20..91)
                     /locus_tag="SDD03_002952"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="SDD03_002953"
     tRNA            complement(113..200)
                     /locus_tag="SDD03_002953"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="SDD03_002954"
     tRNA            complement(225..298)
                     /locus_tag="SDD03_002954"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="SDD03_002955"
     tRNA            complement(312..382)
                     /locus_tag="SDD03_002955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="SDD03_002956"
     tRNA            complement(384..459)
                     /locus_tag="SDD03_002956"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DARDHU010000268          453 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000268 DARDHU010000000
VERSION     DARDHU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.283"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>453)
                     /locus_tag="SDD03_002957"
     CDS             complement(<1..>453)
                     /locus_tag="SDD03_002957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305326.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQ"
BASE COUNT          151 a           76 c           58 g          168 t
ORIGIN      
        1 actgaatttc tgaaaatcga atttttctcc cagttgatag atcaaggggc agaaaacctt
       61 ttattacatt gtgagtgtca ccatcattta caatgtcatt aggaattttg attttagaat
      121 tctgtggagc acctattgga agataataat tcattataat tttgtagtct tcatgaggaa
      181 ataaagtact tttaggattg ctaagatatt ctcgtaccga atctaagaat ggcttatcca
      241 ttgaatcttc atccccattt aaactaaaaa acattcctac aattgctttt gcaactctaa
      301 gtgaattcat tttctttatt gaatgtgtta aatcagactt accattcttt aaacttgtat
      361 tgttataaag gaaattttta ttgtcttctg caaatctaat atattcctca acatactcac
      421 taccaaaaaa actattacat ttactacata acg
//
LOCUS       DARDHU010000269          452 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000269 DARDHU010000000
VERSION     DARDHU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.284"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>452
                     /locus_tag="SDD03_002958"
     CDS             <1..>452
                     /locus_tag="SDD03_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305327.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DARDHU010000270          444 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000270 DARDHU010000000
VERSION     DARDHU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.285"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          139 a           95 c           98 g          112 t
ORIGIN      
        1 tatttttttt acgtttcagt attagtaagg attatttgag ggttatttag agattattta
       61 aaggttatat gtaataagtg gggggagggc tgtcggcgct tggtatgaaa ggcattctcc
      121 gacttccagg tttttgacca ttttctctca acgtggaaaa accgccactt atgagagaaa
      181 atggtcaaaa acctaagcgg gaagtctccg aataacatac caagcgccga cagccaacag
      241 ataaagaata cactactaaa aatagtagta gcataaaatt taaaagggaa aaaatcaacc
      301 cctaccccag tcttggggtc tcaaaactaa ctccccccta accccctctt tcagcctctt
      361 ttgaaagagg gggagaatca accccacggc caacgcccca gtgttcttgg ggctgtccag
      421 aacagaaact tgcgagagag tggg
//
LOCUS       DARDHU010000271          437 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000271 DARDHU010000000
VERSION     DARDHU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.286"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>437)
                     /locus_tag="SDD03_002959"
     CDS             complement(<1..>437)
                     /locus_tag="SDD03_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305328.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQ
                     LTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQK"
BASE COUNT          129 a           86 c           83 g          139 t
ORIGIN      
        1 cttttctgtc ctactttacg aagttcattc atttcacgga tacgcaactc attaaaggct
       61 tgattcatgt gtttgataat atgaaaccga tcaatcacta ctttcgcatt tggcagaata
      121 cgtttggtaa gctggaagta ggcggcgttc atgtctgtga ctaaaaattt tacttcttca
      181 ggattagcgc agcctaagaa atagcttgtt aatcgaggca atttacgcgt aggcaagaca
      241 tcaattaatt gtcctgtttc gccatctgcg caaataaagc tcattttatc ttctatggaa
      301 gcgtgcgaac gaaattcatc aaccatcaat actctgggga gaatcctctt agattgcttt
      361 ggtaagtagc ttttaaactc tttcaacgta cgaataacgg tggtcaaaga tacctgacaa
      421 tttttcgcaa taaaaga
//
LOCUS       DARDHU010000272          428 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000272 DARDHU010000000
VERSION     DARDHU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.287"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>428
                     /locus_tag="SDD03_002960"
     CDS             <1..>428
                     /locus_tag="SDD03_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305329.1"
                     /translation="QKYEAACDKKKKEQALAQKMEEFTACYEDMNNIFVDQEKRLTDK
                     NMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEACCNLFSDTIPFSTSEQ
                     ERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESV"
BASE COUNT          197 a           69 c           74 g           88 t
ORIGIN      
        1 caaaagtatg aagcagcatg tgataaaaag aaaaaagagc aagcattagc acaaaaaatg
       61 gaggaattta ccgcttgtta tgaggacatg aacaatattt ttgtggatca agagaaacgt
      121 ttaacagata aaaacatgaa aaaactcact cgtttttatc aaaacttacc agaaaaacag
      181 caaaaagagg tacaagaaaa atatgagcaa gtaaaaaaag acgtgcaata ttttaaagat
      241 acgcagacag aagaagcttg ttgtaactta tttagcgata caatcccttt ttcaaccagt
      301 gaacaagaac gaaaagaacg gcaacaatcg gtcacatacg aacgttatga aacactgctt
      361 cagcaagcaa caaacataca aaatccaacg aaaaaagaaa cggcattaaa ttatctagaa
      421 agtgtcaa
//
LOCUS       DARDHU010000273          415 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000273 DARDHU010000000
VERSION     DARDHU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.288"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..339)
                     /locus_tag="SDD03_002961"
     CDS             complement(<1..339)
                     /locus_tag="SDD03_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4305330.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DARDHU010000274          399 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000274 DARDHU010000000
VERSION     DARDHU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.289"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DARDHU010000275          394 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000275 DARDHU010000000
VERSION     DARDHU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.290"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..390
                     /locus_tag="SDD03_002962"
     CDS             <1..390
                     /locus_tag="SDD03_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305331.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          155 a           72 c           60 g          107 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaa
//
LOCUS       DARDHU010000276          381 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000276 DARDHU010000000
VERSION     DARDHU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.291"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..94)
                     /locus_tag="SDD03_002963"
     tRNA            complement(21..94)
                     /locus_tag="SDD03_002963"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="SDD03_002964"
     tRNA            complement(108..181)
                     /locus_tag="SDD03_002964"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="SDD03_002965"
     tRNA            complement(209..281)
                     /locus_tag="SDD03_002965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="SDD03_002966"
     tRNA            complement(301..374)
                     /locus_tag="SDD03_002966"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DARDHU010000277          354 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000277 DARDHU010000000
VERSION     DARDHU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.292"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SDD03_002967"
     CDS             6..305
                     /locus_tag="SDD03_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4305332.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
BASE COUNT          123 a           47 c           81 g          103 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatctact atat
//
LOCUS       DARDHU010000278          349 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000278 DARDHU010000000
VERSION     DARDHU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.293"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>349)
                     /locus_tag="SDD03_002968"
     CDS             complement(<1..>349)
                     /locus_tag="SDD03_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305333.1"
                     /translation="RAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPD
                     LFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVS
                     YGFKSFENMRIRIF"
BASE COUNT           95 a           66 c           75 g          113 t
ORIGIN      
        1 aaaagattct aattctcatg ttctcaaatg atttaaatcc gtaggatact cgtttcattg
       61 tctttatatg ggtattcttc gcttctattt ttccattggt gtaaggatag atcatcgcgt
      121 tggtgatgcc ttcttcatag gtcagaaggt tttggagctt ttcccgaaag ccgtcatcta
      181 acgtttcggg aagttctgcc aataaggaga aaaataagtc agggtctttg tctcgaaagg
      241 cttcaactaa ttcatgaaaa tagggatacg cctccttcag ggacgtagaa aagccaagca
      301 atcgatcaat catcatcgct tccgtaagaa atgggtattt tggtgctcg
//
LOCUS       DARDHU010000279          347 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000279 DARDHU010000000
VERSION     DARDHU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.294"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..321)
                     /locus_tag="SDD03_002969"
     CDS             complement(<1..321)
                     /locus_tag="SDD03_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4305334.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           64 g          131 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaattg
//
LOCUS       DARDHU010000280          343 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000280 DARDHU010000000
VERSION     DARDHU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.295"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>343)
                     /locus_tag="SDD03_002970"
     CDS             complement(<1..>343)
                     /locus_tag="SDD03_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305335.1"
                     /translation="PVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKI
                     PHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKF
                     TKEMTHDEAKAY"
BASE COUNT          103 a           67 c           67 g          106 t
ORIGIN      
        1 gataggcttt cgcttcatca tgggtcattt ctttcgtaaa cttatgttta ttggcgttag
       61 ctttgatgtg ggtagaatcc agatacaaat tatcctctgt tacaaaacct gctttaactg
      121 cttgttctaa aatataggca aaaatatcct caaacaccgt cgtttctcta aatcggcgaa
      181 cataattctt tccaaaagtt gagaaatggg ggattttctc ctcgaaggag tagcctaaga
      241 accagcggta agcgacattg gtatcgactt ccttaatggt ttgtcgcatg gaacggatac
      301 cgaataaata ctgaataaac accaatttga tcagaatcac tgg
//
LOCUS       DARDHU010000281          323 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000281 DARDHU010000000
VERSION     DARDHU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.296"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SDD03_002971"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SDD03_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG4305336.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DARDHU010000282          321 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000282 DARDHU010000000
VERSION     DARDHU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.297"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(69..321)
                     /locus_tag="SDD03_002972"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="SDD03_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DARDHU010000283          310 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000283 DARDHU010000000
VERSION     DARDHU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.299"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          126 a           32 c           54 g           98 t
ORIGIN      
        1 taaagagatt gctatattat tatttgttct aaatgattcc tttaaggtgt cactaaaaat
       61 tataaaaaat agtgttgacg cacttttgat gtcatgttat aataacgaag tcgtttggat
      121 gagatagcta ataaagcttg attttatcga aaaaaacttc gaaaaagtta ttgacaaata
      181 acaataaaga tggtatgata atcaagtcgc tgctaatgag tggcaaagac gtttagaaaa
      241 aataaacaaa gaagttgttg acattctctt ctgaatctga taagattaaa gagttgttaa
      301 aacaaaaaga
//
LOCUS       DARDHU010000284          309 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000284 DARDHU010000000
VERSION     DARDHU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.300"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..309
                     /locus_tag="SDD03_002973"
                     /pseudo
     CDS             1..309
                     /locus_tag="SDD03_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DARDHU010000285          308 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000285 DARDHU010000000
VERSION     DARDHU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.301"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(76..>308)
                     /locus_tag="SDD03_002974"
     CDS             complement(76..>308)
                     /locus_tag="SDD03_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305337.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DARDHU010000286          282 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000286 DARDHU010000000
VERSION     DARDHU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.302"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>282
                     /locus_tag="SDD03_002975"
     CDS             <1..>282
                     /locus_tag="SDD03_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG4305338.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DARDHU010000287          261 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000287 DARDHU010000000
VERSION     DARDHU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.303"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            22..>261
                     /locus_tag="SDD03_002976"
     CDS             22..>261
                     /locus_tag="SDD03_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4305339.1"
                     /translation="MIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGKTALDYWH
                     QYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT           98 a           45 c           49 g           69 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt c
//
LOCUS       DARDHU010000288          261 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000288 DARDHU010000000
VERSION     DARDHU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.304"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..118
                     /locus_tag="SDD03_002977"
     CDS             <1..118
                     /locus_tag="SDD03_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG4305340.1"
                     /translation="GNGLYCNKEKCWVNWGQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          103 a           28 c           52 g           78 t
ORIGIN      
        1 tggaaatggt ctttattgta acaaagaaaa atgttgggta aattggggac aatcatggtc
       61 agaagggtta aaacgttggg gagataatct ttttggtagt tttagtggag gacgataatg
      121 acttcaataa acattgatca cacaaaaaaa gtcatacatg aattatacaa tagtttaatg
      181 acacgaccta atcagacgaa agcattatta gatattactg atgttttatt tcaggtatat
      241 aaaaaaattg atacagcaaa a
//
LOCUS       DARDHU010000289          239 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000289 DARDHU010000000
VERSION     DARDHU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.305"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>239
                     /locus_tag="SDD03_002978"
     CDS             <1..>239
                     /locus_tag="SDD03_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4305341.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DARDHU010000290          227 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000290 DARDHU010000000
VERSION     DARDHU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.306"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="SDD03_002979"
     rRNA            complement(<1..>227)
                     /locus_tag="SDD03_002979"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DARDHU010000291          225 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000291 DARDHU010000000
VERSION     DARDHU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.307"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT           91 a           36 c           29 g           69 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattg
//
LOCUS       DARDHU010000292          224 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000292 DARDHU010000000
VERSION     DARDHU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.308"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>224
                     /locus_tag="SDD03_002980"
     CDS             <1..>224
                     /locus_tag="SDD03_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG4305342.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DARDHU010000293          214 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000293 DARDHU010000000
VERSION     DARDHU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.309"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..149
                     /locus_tag="SDD03_002981"
     CDS             <1..149
                     /locus_tag="SDD03_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305343.1"
                     /translation="AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
BASE COUNT           65 a           46 c           43 g           60 t
ORIGIN      
        1 atgcctttaa ggtgtcccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc
       61 tgattaaacg aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt
      121 tacaacaagg tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat
      181 atcggactaa gttttattca aatttgtctt cacc
//
LOCUS       DARDHU010000294          206 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000294 DARDHU010000000
VERSION     DARDHU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.310"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>206)
                     /locus_tag="SDD03_002982"
     CDS             complement(<1..>206)
                     /locus_tag="SDD03_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305344.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DARDHU010000295          205 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000295 DARDHU010000000
VERSION     DARDHU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.denovo.311"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>205
                     /locus_tag="SDD03_002983"
     CDS             <1..>205
                     /locus_tag="SDD03_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305345.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           62 a           49 c           38 g           56 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actct
//
LOCUS       DARDHU010000296        12827 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.076, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000296 DARDHU010000000
VERSION     DARDHU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.076"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(134..577)
                     /locus_tag="SDD03_002984"
     CDS             complement(134..577)
                     /locus_tag="SDD03_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG4305346.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="SDD03_002985"
     CDS             complement(795..2135)
                     /locus_tag="SDD03_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4305347.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="SDD03_002986"
     CDS             2404..3300
                     /locus_tag="SDD03_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG4305348.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="SDD03_002987"
     CDS             complement(3450..4391)
                     /locus_tag="SDD03_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4305349.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="SDD03_002988"
     CDS             4517..5638
                     /locus_tag="SDD03_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4305350.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="SDD03_002989"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="SDD03_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG4305351.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="SDD03_002990"
     CDS             7426..7992
                     /locus_tag="SDD03_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4305352.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="SDD03_002991"
     CDS             8203..9264
                     /locus_tag="SDD03_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG4305353.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="SDD03_002992"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="SDD03_002992"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG4305354.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="SDD03_002993"
     CDS             10177..12159
                     /locus_tag="SDD03_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4305355.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="SDD03_002994"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="SDD03_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG4305356.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         4228 a         2336 c         2656 g         3607 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaa
//
LOCUS       DARDHU010000297         5613 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.129, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000297 DARDHU010000000
VERSION     DARDHU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.129"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(40..1026)
                     /locus_tag="SDD03_002995"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="SDD03_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="SDD03_002996"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="SDD03_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG4305357.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="SDD03_002997"
     CDS             1851..3005
                     /locus_tag="SDD03_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG4305358.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="SDD03_002998"
     CDS             3220..4188
                     /locus_tag="SDD03_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG4305359.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="SDD03_002999"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="SDD03_002999"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG4305360.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="SDD03_003000"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="SDD03_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DARDHU010000298         5122 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.136, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000298 DARDHU010000000
VERSION     DARDHU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.136"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            61..558
                     /gene="dfrG"
                     /locus_tag="SDD03_003001"
     CDS             61..558
                     /gene="dfrG"
                     /locus_tag="SDD03_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG4305361.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(630..2582)
                     /locus_tag="SDD03_003002"
     CDS             complement(630..2582)
                     /locus_tag="SDD03_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG4305362.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2589..2825)
                     /locus_tag="SDD03_003003"
     CDS             complement(2589..2825)
                     /locus_tag="SDD03_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305363.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3060..3863)
                     /locus_tag="SDD03_003004"
     CDS             complement(3060..3863)
                     /locus_tag="SDD03_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG4305364.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(3879..3995)
                     /locus_tag="SDD03_003005"
     CDS             complement(3879..3995)
                     /locus_tag="SDD03_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG4305365.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(4240..5088)
                     /locus_tag="SDD03_003006"
     CDS             complement(4240..5088)
                     /locus_tag="SDD03_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4305366.1"
                     /translation="MIKVGTHKKLTFVLWVLLIGSVGFGIYKNFTAIDTHTVRETEII
                     KQQIVDTNQVESFVKSFAKDYFSWQQSQEAIDKRNEKLTHYLTEELQVLNEEMIRKDI
                     PTSSSVNDIQVWQVSQVNENTFEVLFSVEQVITEDKDKETISSSFHVVVHIDESDNMV
                     IIKNPTMSKKPQKSDYQPKQLESDHTVDTETMDEITSFLETFFQLYPTATEKELTYYV
                     SNHVLPMINKEYVFEELVNPIFTRKDNQVIVNVAVKYLDQETKATQISQFELILEKQD
                     NWKIVK"
BASE COUNT         1539 a          936 c          820 g         1827 t
ORIGIN      
        1 aggttttttg atagaaataa gaaattagat ggtaaattag ttaaaaaggc tgtgttataa
       61 atgaaagttt ctttgattgc tgcgatggat aagaatagag tgattggcaa agagaatgac
      121 attccttgga ggattcccaa ggactgggaa tatgttaaaa atactacaaa gggacatccg
      181 ataatattag gtaggaagaa ccttgaatca atcggaagag ccttacctga cagaagaaat
      241 attattctga cgagagataa ggggtttacc tttaatggtt gtgaaattgt tcattcaata
      301 gaagatgttt ttgagttatg taaaaacgaa gaagaaattt ttattttcgg aggagaacag
      361 atttataatt tgtttttccc ttatgttgag aaaatgtaca tcacaaaaat acatcatgaa
      421 ttcgaaggag atactttttt tccagaagtg aattatgagg aatggaatga ggtatttgcc
      481 caaaaaggga taaagaatga taaaaatccg tataactact attttcatgt atatgaaaga
      541 aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga acagtaaaga taaagcacaa
      601 gagcttggga ttactccaag ctctttttgt catttgaaat aaattatatc tttccaatca
      661 aattgtttat ctgtaatccc taatctttga gcaggtgtca aaaccttttt atccgctgat
      721 ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg taatcgcaaa ttgagcatac
      781 ttcggattga agttagcgta aatatagctc ttcttatcac cacgagctgt tgttaatggt
      841 ctttcaagag atgatatata acgtcttatc tgttgaataa agctattggt cgaatggtca
      901 ttaacattta atagcatctt tgcgatttcg tttggttcca gagcagataa atccgttcta
      961 caatcaactg aataaaatcc tttatcctta gtggctaatg gatgtttgat agggttatcg
     1021 gcatatttgc gatatctttc tccatcttta ctaatagcct cttcgtggaa ttgatgactt
     1081 gttagtaatt cagttagata atgagatgcc aaagtccgta gaaactttgt tttgaatcct
     1141 ctgatatctc cccaatcgag cagttctgct tttgcttgtc cgaattcttt taagcattgt
     1201 tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat gtgcatcgct caaccttaat
     1261 tctttcgtga atactctata aaaggcgttt ctaatagaga agtcatcatc actaatcatt
     1321 cgccattcgt cggcattaac catttgttta attaaccaat agtgagcagt tgtagtataa
     1381 ggagcatcaa catttaaacc atcgacaaat ctactcctat tagtaatttt ccctaattcg
     1441 tgaagataag cattcctatt ttcgctatcg ttagcggatg gttcttgtgg ataataagac
     1501 caatctaacc tatcattttt acgactaaaa gtattgagat ggtcttcttt gaattttctt
     1561 gtatcttcat ttaattcatc cattgaaata ttccaatcat aggcaacatc agaacggaac
     1621 acatatcttg ataagacctc agcagataca actatatacg tttgcatatt aaggtcttca
     1681 aaccccgtat atttctttga accttgtcct ttcttacgga cgttatttag gtagtagtgc
     1741 atcttgtccg tattaagcca catctcgtta aatttctttg tttgaagggg ttgtgtctca
     1801 tatcgttcta aaaactccaa acaacgccta tacagccact ccagcttatg atagtaggtt
     1861 ccaactccaa ttcctaaaat atcacaagta cgactgactg aaacccttcc aacaaccatc
     1921 ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat aagtagtggt ttcttctctt
     1981 tttggtagaa cattagtgaa cttcttacac gccttacatt gatagcgttg agatttgccc
     2041 ctacttttcc cccttttgta aaattgcttc ggctcattga atggggttga ttcttcttcc
     2101 gagcagcctt ctttatgaaa ttgataatct ggctcaacat cttggatgct attgatttct
     2161 ataaggcgtt taatttcttc aacaacagac caattagata aaggggttac tgtacagttt
     2221 tgcgatacac ctctgccaat aggattgtca ttgcagtaca tacctttatg acctttatct
     2281 ttggaactac cagtcatttt atatcgactt ggtttccctt ttacatcttt gaatttgtgc
     2341 tgttctttcc caaaattaca acaaaacgga ttgatgcagt aattatattg aatgggatat
     2401 tcagaaccgt tccatttaaa agacgttggc aagaacaaca ttttgttata ccttgttgca
     2461 aatcgttttg gagttaataa atcatattgt ttagccctat cttttaattc ttcgtttgaa
     2521 atcggaattt taacatctac aatttcatct tccttcgtgg ctaatctttt aagcttggtc
     2581 attgtctatc aacccactct atctttgata cccatttttt caacaatccg cttattactt
     2641 cgcttgttgg caatccattc aataaacttc ttatcgtcta aaatgttcaa taagaattca
     2701 tccactgctt cgctttcagt acgctctgca tattcagcgt attgcttaac tatcaccctt
     2761 acttgctctg atatttccca atccacagac ttagcgttct tattttttgg ctcaagaaat
     2821 tgcataggaa aatgcacccc tttttgctta gtaaattgta accattttaa cacaccaatt
     2881 tgcattccta caataattat ttcacgttcg catttttata tacattttaa cttacatttt
     2941 gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa aacgagtatc aaattcagtt
     3001 gcaatttgat actcatttag gtttacattt tcactttttg tactccaaaa ctgaactact
     3061 taatatgcaa gacgttgtct tttttttgta tattcaattt tgaaaacatt tccgcaaagt
     3121 tcagacggac ctcgatgtgt tgatgaataa aatttattag taataacttc tataaggtta
     3181 tcaatcccta tattactttt tgcacttcca tgataaagag ggaacagaga acaattctga
     3241 aatcttatgc tttcctcttg ttcgagttcc aatgcttcta atgatttacc ggacatatat
     3301 ttctctaaaa ggtcatcgtt tccctctatt accgtatccc attgttcaga ttcggtaaag
     3361 ttcgtcacac acatattagg atacagttct accttctgtt tgattacaat ttcggcagaa
     3421 agtttctctt taatatcctg ataaaccgtt gataaatcaa ttccattttg gtcaatctta
     3481 ttgataaaaa agattgtggg aatccccatt ttcctaagtg catgaaataa tatacgagtt
     3541 tgtgcttgta cgccatcttt tgcagaaatc agtagaattg ccccatctaa aactgataat
     3601 gaacgatata cttctgctaa gaaatccata tgtcctggcg tgtctatgat gttcaccttc
     3661 gtattttccc actgaaaaga ggttattcct gtctgaattg taattcctct ctgacgttct
     3721 aaaagcgtat tatccgtcct cgttgtacct ttgtccacgc ttcctaattc tgtaatcgct
     3781 ccactgttat ataataagct ttctgttaag gtagtttttc ctgcatcaac atgagctaaa
     3841 actccaatat taataatttt catgtgattt tcctccattc aaaaacccaa aagggcataa
     3901 aaatcccagt gataaatact tttatcactg ggatttttat gcataaccat aggtatacaa
     3961 agcatacaga tattctctgg atactttaga atcacatgat aaaggtattc ttaaactggg
     4021 tacaaaaaac taagccctcc taaaaaagga catctaatta tttgttcccg ctatcaaatt
     4081 gacagtttat ttaagaatac cttgccgcat atttattaac tccttttaaa tagtcactta
     4141 aataatagca cgtaagagca tatttgtaaa ggaatctcca attttttatc aaaaagagta
     4201 catgattaca aagtatctgt aatcatgtac caatatttgt tattttacaa tcttccaatt
     4261 atcttgtttt tctaatatca actcaaattg cgagatttgg gttgcctttg tttcttgatc
     4321 taaatatttc acagcgacat tcacgattac ttggttatct tttctggtaa agataggatt
     4381 caccaattcc tcgaaaacat attccttgtt aatcataggt aacacatgat tacttacata
     4441 gtaagtcaat tctttttctg tggcggtagg atagagctgg aagaacgttt ctaaaaagct
     4501 agtgatttcg tccatcgttt ctgtatctac tgtatggtca ctttctagct gtttgggttg
     4561 gtagtccgat ttttgaggtt tcttactcat tgtcggattt ttgataataa ccatattatc
     4621 tgattcatct atatggacaa ccacatgaaa gctagacgat atagtttcct tgtctttgtc
     4681 ttcggtaata acttgttcaa cagaaaacag tacctcaaag gtgttctcat tcacttgaga
     4741 aacctgccac acctgtatat cgttcacact agaactagta ggaatatcct tacgaatcat
     4801 ttcttcattt aatacctgca attcttccgt caaataatgg gtcaattttt catttcgttt
     4861 atcaatggct tcttgggatt gttgccacga aaagtagtct ttcgcaaagg atttcacaaa
     4921 actttctact tggttggtat cgacaatctg ctgtttaatg atttctgttt cacggacagt
     4981 atgagtgtca atggcagtaa aatttttata gattccaaat ccgacactac caatcaataa
     5041 aacccataaa acaaaggtta atttcttatg cgtgccaact ttaatcatcg gaacttttct
     5101 ttgcttgctc ggttttttag at
//
LOCUS       DARDHU010000299         4647 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.145, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000299 DARDHU010000000
VERSION     DARDHU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.145"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            96..1211
                     /locus_tag="SDD03_003007"
     CDS             96..1211
                     /locus_tag="SDD03_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG4305367.1"
                     /translation="MQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEI
                     TNQLTDGQTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDL
                     YASVMIAQAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAA
                     FRKYPSYKESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNY
                     GSTLNNVIETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEG
                     IAWQAPNSGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRL
                     FNPNEKTGTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1698..2162
                     /locus_tag="SDD03_003008"
     CDS             1698..2162
                     /locus_tag="SDD03_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4305368.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2146..2775
                     /locus_tag="SDD03_003009"
     CDS             2146..2775
                     /locus_tag="SDD03_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4305369.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            3129..4607
                     /gene="msr(C)"
                     /locus_tag="SDD03_003010"
     CDS             3129..4607
                     /gene="msr(C)"
                     /locus_tag="SDD03_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG4305370.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         1595 a          814 c          940 g         1298 t
ORIGIN      
        1 acacaacagt gctagctgaa gaatcgacag aaactgtcgc aactcagaca tcgagttctt
       61 cagaaacagg aattgaaaac gatacagaaa gtactttgca gtcagctact gtctcagaaa
      121 atacaatttc aaccgaagaa ccaacaactg aaacaacaga acaaacacaa accgaagaca
      181 gtacatcagc agaagctcag aacaatccag agaatgcaga agaaatcacg aaccaattga
      241 cagatgggca aacattgaca catacccatc aagcagattt gtctgaattc gaaaactata
      301 ctggctatgc tttttatgct cgctcatctg ctaattctca acaagcgttc attgatagta
      361 ttgcctcaac tgcacaaagt ttggcaagtg ccaatgactt atatgcatca gtcatgattg
      421 ctcaagccat cgttgaaagt ggctggggga acagtacttt ggcttctgcc cctaattaca
      481 atctatttgg gattaaagga agctataatg gccaatctgt tacgatgcct acttcggaat
      541 atgtgaatgg gcaatggatt acggtgaatg ccgcttttag aaaatatcct tcgtacaaag
      601 aatcattgca agataacgta acggtattga agacgacttc ttttcagcca ggcgtttact
      661 actattccgg tgcatggaaa agcaatacga actcttacaa agatgctacg gcttggctaa
      721 caggtcgtta tgccactgcg cctaattatg gttctacatt gaacaatgta atcgaaacct
      781 ataatctgac acagtatgat acagcaccaa cttcaacttc tccgatgtat cgtctgtata
      841 accgtcatac aggagaacat ttatatacat tgaatgccgg agaaaaaaat tatctgccaa
      901 ctgtaggctg ggaatatgaa gggattgctt ggcaagcacc aaacagcgga caacctgtct
      961 accgtttata caatccttac agtggagacc atcattacac aatggcacaa tctgagatca
     1021 actctttaac aaaaatcggt tggcgttatg aaggtctaag tttttattct ggtggttcca
     1081 agccaattta tcgactattt aatccaaatg aaaaaactgg tacccatcac tataccttga
     1141 gtgctaagga aagagattgg ctaacaccga tgggctggag atatgaagga ataggatttt
     1201 atggttatta aaaagaggta gtgaaaaagc tgccctgcat caagtagtaa gaacagccaa
     1261 acaaaaacag attttcatgt tttttgtttg gttgaactta ttaccgcaga tgcaagtttt
     1321 tgaacaccgt ttactcaaaa aaggttgtga caacatcttg tcataacttt tttattactg
     1381 tttaagcata ctgatccaat ttctaatatt cgacgggatt cttgcaaaag gcgatttcgg
     1441 aaatttttgg cggtgtaaaa ttatctactg cagaagagct atcttctata gtttgataag
     1501 aatcaataat cttggtttta gcgttgtgct ttctttatca ttttttccgt tcattctaca
     1561 aaaaatttct tttctctata cttttctatt gagagcgttt tttttattac acagctgatt
     1621 ctttgacttt tttctttttt tattctatta ttgacaagtc aatcgttgac gagtcaatta
     1681 ttagaaagga ggaaattatg tctttagaag agattagtca ccttttatat caaatcaaat
     1741 tagccgatca aaaaatcgtc cagatctttg aagaaaatat cggtataagt cttactagat
     1801 acgagatcat gatgagactg aaagaagaaa atccattgct acaaagcagt ttgcaagaaa
     1861 agttgcagat tgatcaagga gcagttactc gtcatctgaa ggtattggaa gcaaatggct
     1921 atgtttcgcg tcagagaaat cctcagaaca accgggaagt gtatgtagcg ctaacagaaa
     1981 aagcccagaa gaaaatcaag cattgtgaaa tggaccataa agatacaggt tccgtgctat
     2041 ccccggcttt cagcgatgta gaaatgaaac aatttctaac acttttagat aaattgaata
     2101 cccaactttg caatataaca acagaaacga aagaggttaa taataatgga acaaaaaaat
     2161 gatttttcaa caattatgac tggtagaaga tccgttcgcg tatatgatga aacatacaaa
     2221 atcccacacg aagaaatgct ggaaatgatc aaagaagcga cgactgcacc ttcttctgtc
     2281 aatatgaaac catggcgctt tgtggtcgct gaaagcgagg aagcaaaaga aatcttgcgt
     2341 cctttgatcc gtttcaatac gagacaaaat gatacttctt ctgcaatggt catgattttt
     2401 ggcgatatgc aatgttacga atatggcgaa gaaatctaca accaagctta cgaatcaggg
     2461 aagatgccaa aagaagtcag agatcaacaa ttagctgcga tcattcctta ttacaagagt
     2521 ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt ctagcttagc ggctatgcaa
     2581 ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc caattggtgg attcgaagca
     2641 gatcagttgg cagaggcatt tggtttggat aaagaccgtt atgttccagt tattatttta
     2701 tcaataggaa aagcggtaga agaaggttac gagtctgttc gtttagcacc agaaaagatc
     2761 actatgttcg aataaaaggt tttcagttac tattcagtag ctattgcttt ttttgaatcg
     2821 tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg aaattacgtt
     2881 tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa caatttcatg
     2941 gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat gacagatttg
     3001 gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag tttttttgca
     3061 aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga aataggagtg
     3121 taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc tttggcaacc
     3181 agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata gggatcatag
     3241 gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa ctttttcccg
     3301 atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta acctatctcg
     3361 ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga ctggaagaaa
     3421 cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata cgtcttcttt
     3481 cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat agaaaaagta
     3541 ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt gttagtcatg
     3601 atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac ggagaaatcc
     3661 gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag aaaaagactc
     3721 agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa tcgatccaag
     3781 aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa
     3841 tcagactgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa
     3901 agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc aagccgcagc
     3961 agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc cgctatccca
     4021 tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta aatggtgatt
     4081 ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa
     4141 ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg aaagtaagct
     4201 ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc attcgttttg
     4261 tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat cacttagggt
     4321 ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa gcgacccgtc
     4381 tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat gaaccgacta
     4441 attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata tatccgggaa
     4501 cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat gaagtttatg
     4561 aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg
     4621 attttcgtga caaatttaaa ggagtgg
//
LOCUS       DARDHU010000300         3218 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.171, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000300 DARDHU010000000
VERSION     DARDHU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.171"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1135)
                     /locus_tag="SDD03_003011"
     CDS             complement(<1..1135)
                     /locus_tag="SDD03_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305371.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIF"
     gene            complement(1849..2139)
                     /locus_tag="SDD03_003012"
     CDS             complement(1849..2139)
                     /locus_tag="SDD03_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4305372.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(2347..3039)
                     /locus_tag="SDD03_003013"
     CDS             complement(2347..3039)
                     /locus_tag="SDD03_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4305373.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1018 a          577 c          449 g         1174 t
ORIGIN      
        1 aaaaaataga agttttctta ttgtcaaaac ttaaataaat tccaacaatt aaacatagta
       61 ataatgccaa gactactaaa gtagctatcc aagtacctgg ataaaaaagt gacagtttcg
      121 ttcctaactc tgtaaagaga ttattcaggt tactactact ccaaatcatt gcccttttat
      181 ttccattgac gtaagtaaag ctttgaccca ttgtattacc aaaataaata atcaggttta
      241 agaaaaacgg taatgcagtc aatatacgca cataatagtt ttgacatttc ctatatgtta
      301 gaaggaaaat taagaaaaat agcaataaaa ataagatatt catgtctaaa aaaaacggct
      361 ttccaaatga tgaaatacct aaatctaact tattaagaaa gttgaaattt ttatattcag
      421 gaaaccattt attaatttca tgtgcagcac gtaatgcatt ccctggagtt gtcaaagaga
      481 aaattaattc tgctaagctt attattgaaa atactgataa cttataatta tatcgtccat
      541 tgacaatcaa atataaacta actatacttg ttagaacaaa aaaacaggcg ttgacttgtt
      601 cttgattagt taaaaatatc aataaaggaa tacttaccca atatattttt ctatttattt
      661 cttctcctct taagagttga aaaaacggat aaaaagttag taaactaaat gctactggcc
      721 actgatagtt caaggtggtt gctctccaac cggtttcccc cattactgtc aatggtatta
      781 aacagtatat agagcaaacc aataataaat ttttaacatt tattttctca aaaacatact
      841 tgcaaagtaa tactacaatt cctaacataa tcgtagaatt taataatttc catagcataa
      901 agtgtttaga aaagaacatt aagaatagtt ctataataat tcttgaactc catgtcttat
      961 aatgttctat cgaagcaccc aaatagccaa gttcatctaa tctacttgca taaactacgt
     1021 catccgctct catagtcggc atatacatat taaagagtaa aaagataccc caaatcccta
     1081 ttagtaacaa gatcagttct ttgttattcg ttatttttct attatgtttg ttcattttta
     1141 tactccttct tatatttcct aatagagtat aacatagctt taagcattac ttaaagataa
     1201 cttaaaagtt atcttttaaa tacttaattg ttaaatacct tatttgatgc aaaagaaatc
     1261 tacgatagtc aacaaaaaaa ttttcattat atccagaaaa atttttctta gttaaggacc
     1321 tatggattgc agtaatgcag aattcgtcga ataaactgcc aaagacatac aatcatattg
     1381 cttttaaaat tgacgaacaa gatatagatt cgtttgtctc aaaaatccaa atgttaggtt
     1441 taactgtcga accaggacgt tctcgtgtga aaggcgaggc ttcttcaatt tacttttacg
     1501 actatgataa tcacctcttc gagttacata ccggtactct ccaggaacgt ttaaaatcct
     1561 acaattccac tacctgagac tagtaatgac tagtctcttt ttatggcctc tgatcccccc
     1621 attgagcgaa tactaatagg gcttcgaatt gatctgttca atctttaagc aatatccctc
     1681 aaatgagttt ggaaagttta gtgattcatc acaaatactt aaatacatgt agttatatat
     1741 agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa atcccatcgt ctgcatccat
     1801 ctttccaaaa caagcggcta ggacattttt gtctcagccg cttgttttct agtttattct
     1861 ttcatcttgt cggacctttt ttcttgggta tagacttttc tcatattcta ccacaatttt
     1921 gggtttccct aacacatgat aggatctcac aacaaattgc tttctattat tataataacc
     1981 tgtaacgact aacttcatcc cttcgtctac ttcagctagg aagttcaaac tatgggaagc
     2041 aatcaaacaa ttgaccccat ttaaactgaa acggatcaac ggtctggcac taacttgcaa
     2101 agtacggatc ttatctactg ttccatattt tttctccata accttcacct caataccatt
     2161 atacgaacat acattccctt tgtaaagaag aaactctgtt ttatcttttt gactttttgt
     2221 ctattacctt tcttaaaatc atattggcac ttcctgcgaa gatgaaggac atgtactaaa
     2281 aaaaagaggc agcagtatag attactgttg cttctttact aatttaggtt tacttgttaa
     2341 gaaagattat ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc
     2401 cacttcatct tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc
     2461 agttgcttcg tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc
     2521 ttgtgtcagt gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag
     2581 ttttgcttgt gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc
     2641 atttttttgg ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg
     2701 tgcatccttg ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt
     2761 ttggttttct aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg
     2821 caatgtctgt tctttttgtg ctagtcgagc ggtaagatct gtaagcgctt gctctttatc
     2881 tgtcaatgta tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc
     2941 accccacaaa ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa
     3001 tgcaacgaca atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa
     3061 tacttttaca tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca
     3121 tgtgtaattt taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt
     3181 aaaaaatctt ttttatagag aaaaatgcag gttaatac
//
LOCUS       DARDHU010000301         2938 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.178, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000301 DARDHU010000000
VERSION     DARDHU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.178"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..249
                     /locus_tag="SDD03_003014"
     CDS             <1..249
                     /locus_tag="SDD03_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4305374.1"
                     /translation="SIAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSR
                     SEEILIKTLKRRVMELEKENKKLQNQIQKLYGDLYNKE"
     gene            403..1185
                     /locus_tag="SDD03_003015"
     CDS             403..1185
                     /locus_tag="SDD03_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG4305375.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1311..2042)
                     /gene="erm(A)"
                     /locus_tag="SDD03_003016"
     CDS             complement(1311..2042)
                     /gene="erm(A)"
                     /locus_tag="SDD03_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG4305376.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2100..2159)
                     /locus_tag="SDD03_003017"
     CDS             complement(2100..2159)
                     /locus_tag="SDD03_003017"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG4305377.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2551..>2938
                     /locus_tag="SDD03_003018"
     CDS             2551..>2938
                     /locus_tag="SDD03_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4305378.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGR"
BASE COUNT         1002 a          428 c          539 g          969 t
ORIGIN      
        1 tcaatagcaa aggaagctaa tgtttctaaa tcatggcttt ataaggaaca cgatattagg
       61 caaagaatcg aatcccttcg tgagcgtcaa ataacagcaa atgtagtctc aaaacccaag
      121 aaaagttctc gttcggagga aatccttatt aaaaccttaa aaagaagagt aatggaatta
      181 gaaaaagaaa ataaaaaatt acagaaccaa attcaaaaat tatatggaga tctgtataat
      241 aaagaataat tattaatctg tagacaaatt gtgaaaggat gtacttaaac gctaacggtc
      301 agctttattg aacagtcatt taggtatatg tccaatctag ggtaagtaaa ttgagtatca
      361 atataaactt tatatgaaca taatcagtta cgaggtgaaa tcatgagcaa tttgattaac
      421 ggaaaaatac caaatcaagc gattcaaaca ttaaaaatcg taaaagattt atttggaagt
      481 tcaatagttg gagtatatct atttggttca gcagtaaatg gtggtttacg cattaacagc
      541 gatgtagatg ttctagtcgt cgtgaatcat agtttacctc aattaactcg aaaaaaacta
      601 acagaaagac taatgactat atcaggaaag attggaaata cggattctgt tagaccactt
      661 gaagttacgg ttataaatag gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa
      721 tttatatacg gtgagtggct caggggtgaa tttgagaatg gacaaattca ggaaccaagc
      781 tatgatcctg atttggctat tgttttagca caagcaagaa agaatagtat ttctctattt
      841 ggtcctgatt cttcaagtat acttgtctcc gtacctttga cagatattcg aagagcaatt
      901 aaggattctt tgccagaact aattgagggg ataaaaggtg atgagcgtaa tgtaatttta
      961 accctagctc gaatgtggca aacagtgact actggtgaaa ttacctcgaa agatgtcgct
     1021 gcagaatggg ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga
     1081 aaaggctatc ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca
     1141 ctcgttaagt atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca
     1201 ataacaggtg cttactttta aaactactga tttattgata aatattgaac aatttttggg
     1261 aagaataaag cgtcctcttg tgaaattaga gaacgcttta ttactttaat ttagtgaaac
     1321 aatttgtaac tattgaaaat agaaagaaat tgttccttcg atagtttatt aatattagtg
     1381 acatttgcat gcttcaaagc ctgtcggaat tggtttttag tgaaaagaac acgatattca
     1441 cggtttaccc acttataaac aaaagatcga tactttttgt agtccttctt tgaaatcaat
     1501 ggttgatgtc gttcaagaac aatcaataca gagtctacac ttggcttagg atgaaaatat
     1561 agtggtggta cttttttgag catttttata tccatctcca ccattaatag taaacccaaa
     1621 gctcgttgca gattttgcaa tcttttcgca aatcccttct caacgataag atagctatat
     1681 ttagcctgac tttcaaaggt aattcttttg acaatatccg tactgatgtt ataaggaata
     1741 ttaccatata tcttatagtt tatatgtttt gggaaggaaa attttagaat atccgtttga
     1801 atcactttta tattctcaga ggggtttacc gcttctttag tcacttgaca taagcctcca
     1861 tcaatttcta tagcagtaac tgatcgactc attttgacta gctctttggt aaaatgtcct
     1921 tttcctgatc cgatttctat tacgttgtct tgtttactga tattcgtgtg attcaatatt
     1981 tcttttacat gctttttaga agtaataaaa ttttgcgtgt ctttagggtt tttctggttc
     2041 attataacct tctccttact ggttataatg aactgacatt aagcgttcat tataaccaat
     2101 taattttgat ttggttgata acgaacttta ttaataacaa atatagaaaa agtacccata
     2161 cttttatctc ctttccattt ttttcattat gaatgagata aagtaatttc tactactgcg
     2221 atactggtgc acataattga aacctcctta tgtaactgaa tatgattgta tcatcttttc
     2281 attttagaat aaacctttat atgtaatttt gcacttgaca cttcagatgg acgttttctt
     2341 caaaataata tgttttgaaa cttgataaga gaagccactg tctttactaa aaagtaaagg
     2401 ctgcgataaa ggacagtgac tttaataaag ttgcactgtt gtattgtctt tggataaaat
     2461 tgtctaaagg gaagttttat ccccaacgaa aaaacatcta aagtggtaag taatgaaaga
     2521 atgtcgataa agtaggaatg ggggatcttt ttgaatacaa tagaatcaca gtttgataag
     2581 gttgcagaag attacgattt cgtgaatgag cttttgaatg attattcatt ctttgtgtct
     2641 aatatgtctc caaagaaagg cagagcatta gatatcggat gtggctcggg tttgttagtg
     2701 gagaaattag caagttatta tgatgaagtg gtagggattg atatttctaa tcaaatgctc
     2761 gatcttgcca aatctaaacg tcaactaaca aatacggtct atctgaatat gaatgcagaa
     2821 caacttaatt ttaatgagaa gtttgatttt attgtaagcc gaacaacctt tcatcatttg
     2881 gatgatatag ccagcgtaat acagcaaatg aaggaactgt tgaatgagga aggaagaa
//
LOCUS       DARDHU010000302         1504 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.212, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000302 DARDHU010000000
VERSION     DARDHU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.212"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            262..345
                     /locus_tag="SDD03_003019"
                     /pseudo
     CDS             262..345
                     /locus_tag="SDD03_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="SDD03_003020"
     CDS             394..477
                     /locus_tag="SDD03_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG4305379.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="SDD03_003021"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="SDD03_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG4305380.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DARDHU010000303         1399 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.220, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000303 DARDHU010000000
VERSION     DARDHU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.220"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            270..1310
                     /locus_tag="SDD03_003022"
     CDS             270..1310
                     /locus_tag="SDD03_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4305381.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT          473 a          206 c          261 g          459 t
ORIGIN      
        1 caattttttc tataagttat attatcataa taccacaaaa tcacaaattt tcattcaatt
       61 atcattttca attgcatttt ataaatcatt ttgtatgtaa tttggtatgt aagttaaaat
      121 gaaaaaagta gtccattttg aagtacattt tgtcattttg tactccaaaa ctgaactact
      181 tacttgcata tatacgcctt tatagtggag tactacattt acgagattcg gttagagtat
      241 cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt gaattatgta
      301 agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt ttgaagttaa
      361 atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa aatccgcacc
      421 ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg ttgcttgatg
      481 cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac
      541 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
      601 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
      661 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
      721 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
      781 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
      841 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtacg
      901 gtttatacta tagccctgtt agtactccag cagattttcg gatgcttact cctattgtac
      961 tggagcaagc ctttagaaaa gctggaacag aattgttaga gccatatctt agttttaaag
     1021 tttatgcacc acaggaatat ctttcacggg catataacga tgctcccaaa tattgtgcaa
     1081 atatcgtaaa tactcaactg aaaaataatg aggtcattat tattggagaa attcctgctc
     1141 gatgtattca agattatcgc aatgatttaa ctttttttac aaatgggctt agtgtttgtt
     1201 tagcagagct aaaaggatat caggttacca ctggcgaacc tgtttgccag acccgtcgtc
     1261 taaatagtcg gatagataaa gtaagatata tgttcaataa aataacttag tgcgttttat
     1321 gttgttatat aaatatggtt tcttattaaa taagatgaaa tattctttaa tatagatttg
     1381 aattaaagtg gaaaggagg
//
LOCUS       DARDHU010000304         1350 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.222, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000304 DARDHU010000000
VERSION     DARDHU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.222"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..298
                     /locus_tag="SDD03_003023"
                     /pseudo
     CDS             47..298
                     /locus_tag="SDD03_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="SDD03_003024"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="SDD03_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG4305382.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DARDHU010000305         1081 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.238, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000305 DARDHU010000000
VERSION     DARDHU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.238"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            366..914
                     /gene="aac(6')"
                     /locus_tag="SDD03_003025"
     CDS             366..914
                     /gene="aac(6')"
                     /locus_tag="SDD03_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG4305383.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          375 a          157 c          215 g          334 t
ORIGIN      
        1 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
       61 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
      121 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
      181 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt
      241 attttataag taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt
      301 tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa
      361 gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg
      421 atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag
      481 aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat
      541 ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag
      601 aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag
      661 tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa
      721 cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc
      781 ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac
      841 caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac
      901 caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag
      961 gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct
     1021 acttttggaa caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga
     1081 a
//
LOCUS       DARDHU010000306          892 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.249, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000306 DARDHU010000000
VERSION     DARDHU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.249"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..821
                     /locus_tag="SDD03_003026"
                     /note="aph(2'')-Ia"
     CDS             <1..821
                     /locus_tag="SDD03_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEG4305384.1"
                     /translation="IGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNL
                     ETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQM
                     HGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGK
                     KCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDI
                     LRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          381 a           63 c          146 g          302 t
ORIGIN      
        1 taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac atttttaaaa
       61 caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata tataattttt
      121 taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg tatattagtg
      181 atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca ccagaaattt
      241 attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc agttttttaa
      301 gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat aataaacaaa
      361 atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta actgatatag
      421 aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt tttgagggta
      481 aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat ggcaataata
      541 gattaactgg aataattgat tttggagatt ctggaattat agatgaatat tgtgatttta
      601 tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa gatatattaa
      661 gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata gttgaagaat
      721 attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa
      781 atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat atatgaaaag
      841 ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata ag
//
LOCUS       DARDHU010000307          569 bp    DNA     linear   BCT 08-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4435
            SAMEA114569963-rid20124543.guided.270, whole genome shotgun
            sequence.
ACCESSION   DARDHU010000307 DARDHU010000000
VERSION     DARDHU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569963
            Sequence Read Archive: ERR12203788
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001974099.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001974099.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 11:53:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,027
            CDSs (total)                      :: 2,969
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 24 of 68
            Pseudo Genes (incomplete)         :: 30 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569963-rid20124543.guided.270"
                     /isolate="VRE4435"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>569
                     /locus_tag="SDD03_003027"
     CDS             <1..>569
                     /locus_tag="SDD03_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4305385.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLV"
BASE COUNT          227 a           53 c          104 g          185 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaa
//
